JP7300195B2 - Effervescent topical skin preparation - Google Patents

Description

TECHNICAL FIELD The present invention relates to an effervescent external preparation for skin.

BACKGROUND ART Conventionally, external preparations for skin containing synthetic or natural extracts having a blood circulation-promoting effect on the skin have been used.

A small amount of these extracts is not effective enough, and a large amount of these extracts is too irritating to the skin. is done. Many of these generate carbon dioxide through reactions between acidic substances and carbon dioxide-generating substances. Carbon dioxide gas-effervescent topical preparations for skin have conventionally been formulated with effective plant extracts such as pine bark extracts (see Patent Document 1).
On the other hand, it has been known for a long time that carbon dioxide gas has a blood circulation-promoting effect, and for example, pack cosmetics with a warming sensation containing pyrogens are known (see Patent Document 2).

JP 2014-88345 A JP-A-08-268828

However, in some cases, plant extracts such as pine bark extracts are less effective, and furthermore, whitening the skin color, increasing the moisture content of the skin, reducing the amount of water evaporation, and improving the elasticity of the skin. It has been desired to develop a carbon dioxide effervescent topical skin preparation that is more effective in improving skin conditions.
In addition, in the conventionally known packs containing carbon dioxide gas, although the foamability of carbon dioxide gas, the persistence of foam, physical stimulation, etc. have been studied, the improvement effect of carbon dioxide gas on the skin condition has not been found. The study has not yet been sufficiently conducted, and the usability and stability of the product, as well as the feeling of use after rinsing, have not been sufficiently investigated.

Therefore, in the present invention, by blending a new component in the carbon dioxide foaming external preparation for skin, the skin condition is improved, the usability, safety and stability are excellent, and the feeling after use is good after rinsing. , various studies were conducted on active ingredients that can further enhance the effects of carbon dioxide gas.
As a result, the present inventors have found that the effects of carbon dioxide gas can be enhanced by adding a specific active ingredient to various carbon dioxide gas-effervescent skin preparations for external use, thereby solving the above-described problems, and the present invention has been completed.

The present invention provides a carbon dioxide effervescent external skin preparation containing an acidic substance, a carbon dioxide-generating substance that reacts with the acidic substance to generate carbon dioxide, and a thickener in the same agent or two or more agents, Furthermore, ascorbic acid glucoside, allantoin, estradiol, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhizic acid, stearyl glycyrrhetinate, tranexamic acid, d-camphor, dl-α-tocopherol acetate, dl-α-tocopherol nicotinate, D-pantothenyl alcohol, pantothenyl ethyl ether, ethinylestradiol, salicylic acid, biotin, resorcinol, trichlorocarbanilide, triclosan, benzethonium chloride, sulfur, photosensitizer No. 201, phenol, pyridoxine hydrochloride, pyridoxine, zinc oxide, arbutin, phosphorus Carbon dioxide effervescent topical preparation for skin containing at least one selected from L-ascorbyl magnesium acid, ascorbyl dipalmitate, placenta, retinol and retinol palmitate (however, if the carbon dioxide effervescent topical preparation for skin consists of one agent, , to be mixed with a water-containing substance at the time of use, and when it consists of two or more agents, one of the agents shall contain water) (hereinafter simply referred to as "external preparation for skin"). be.

The present invention also provides a carbon dioxide effervescent external skin preparation (hereinafter simply referred to as "external skin preparation kit" or "kit"), which is a two-agent kit according to any one of the following (1) to (3). It is something to do. In each of the following kits, the first agent and the second agent are mixed at the time of use.
(1) a first agent that is an acid-containing composition containing an acidic substance and water;
a second component that is a base-containing composition containing a carbon dioxide-generating substance that reacts with the acidic substance to generate carbon dioxide, wherein the first component and/or the second component contains a thickener; , Furthermore, the first agent and / or the second agent is ascorbyl glucoside, allantoin, estradiol, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhizinate, stearyl glycyrrhetinate, tranexamic acid, d-camphor, dl-α acetate - tocopherol, dl-α-tocopherol nicotinate, D-pantothenyl alcohol, pantothenyl ethyl ether, ethinyl estradiol, salicylic acid, biotin, resorcinol, trichlorocarbanilide, triclosan, benzethonium chloride, sulfur, photosensitizer No. 201, phenol, It contains at least one selected from pyridoxine hydrochloride, pyridoxine, zinc oxide, arbutin, magnesium L-ascorbyl phosphate, ascorbyl dipalmitate, placenta, retinol and retinol palmitate.
(2) a first agent that is an acid-containing composition containing an acidic substance;
a carbon dioxide-generating substance that generates carbon dioxide by reacting with the acidic substance and a second agent that is a base-containing composition containing water, wherein the first agent and/or the second agent include a thickener. Further, the first agent and / or the second agent contains ascorbic acid glucoside, allantoin, estradiol, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhizinate, stearyl glycyrrhetinate, tranexamic acid, d-camphor, dl acetate - α-tocopherol, dl-α-tocopherol nicotinate, D-pantothenyl alcohol, pantothenyl ethyl ether, ethinyl estradiol, salicylic acid, biotin, resorcinol, trichlorocarbanilide, triclosan, benzethonium chloride, sulfur, photosensitizer No. 201, It contains at least one selected from phenol, pyridoxine hydrochloride, pyridoxine, zinc oxide, arbutin, magnesium-L-ascorbyl phosphate, ascorbyl dipalmitate, placenta, retinol and retinol palmitate.
(3) a first agent that is an acid- and base-containing composition containing an acidic substance and a carbon dioxide generating substance that reacts with the acidic substance to generate carbon dioxide;
and a second agent that is a composition containing water, the first agent and / or the second agent contains a thickening agent, the first agent and / or the second agent is ascorbic acid glucoside, Allantoin, estradiol, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhizic acid, stearyl glycyrrhetinate, tranexamic acid, d-camphor, dl-α-tocopherol acetate, dl-α-tocopherol nicotinate, D-pantothenyl alcohol, Pantothenyl ethyl ether, ethinyl estradiol, salicylic acid, biotin, resorcinol, trichlorocarbanilide, triclosan, benzethonium chloride, sulfur, photosensitizer No. 201, phenol, pyridoxine hydrochloride, pyridoxine, zinc oxide, arbutin, L-ascorbyl magnesium phosphate , ascorbyl dipalmitate, placenta, retinol and retinol palmitate.

The present invention also provides a carbon dioxide foaming external preparation for skin (hereinafter also simply referred to as "composition for external preparation for skin" or "composition") which is a one-part composition of the following composition to be mixed with a water-containing substance at the time of use: It provides.
Composition: Contains acidic substances, carbonates and thickeners, and also ascorbic acid glucoside, allantoin, estradiol, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhizinate, stearyl glycyrrhetinate, tranexamic acid, d-camphor, dl-α-tocopherol acetate, dl-α-tocopherol nicotinate, D-pantothenyl alcohol, pantothenyl ethyl ether, ethinyl estradiol, salicylic acid, biotin, resorcinol, trichlorocarbanilide, triclosan, benzethonium chloride, sulfur, photosensitizer 201 No., phenol, pyridoxine hydrochloride, pyridoxine, zinc oxide, arbutin, L-ascorbyl magnesium phosphate, ascorbyl dipalmitate, placenta, retinol and retinol palmitate.

According to the present invention, by blending a specific active ingredient, the effect of carbon dioxide gas is enhanced, so that it whitens the skin color, increases the moisture content of the skin, reduces the amount of moisture loss, and improves skin elasticity. In addition to improving the skin condition, it has excellent usability, safety, and stability such as ease of mixing and application, and the feeling of use after rinsing is good, and the effect of carbon dioxide gas, etc. can be further increased. In addition to improving the condition of stratum corneum cells and/or promoting skin turnover, the carbon dioxide gas foaming skin has new effects such as excellent skin cell activating action, smaller skin pores, and smoother skin texture. An external preparation, and a kit and composition from which the skin external preparation can be obtained can be provided.

FIG. 1A shows the skin condition measurement results of Comparative Examples 1 to 3 and Examples 1-1 to 1-10, where (a) is the result of color difference and (b) is the result of water loss. FIG. 1B shows the skin condition measurement results of Comparative Examples 1 to 3 and Examples 1-1 to 1-10, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 2A shows the skin condition measurement results of Examples 1-11 to 1-20, where (a) is the result of color difference and (b) is the result of water loss. FIG. 2B shows the skin condition measurement results of Examples 1-11 to 1-20, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 3A shows the skin condition measurement results of Examples 1-21 to 1-30, where (a) is the result of color difference and (b) is the result of water loss. FIG. 3B shows the skin condition measurement results of Examples 1-21 to 1-30, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 4A shows the skin condition measurement results of Examples 1-31 to 1-35, where (a) is the result of color difference and (b) is the result of water loss. FIG. 4B shows the skin condition measurement results of Examples 1-31 to 1-35, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 5A shows the skin condition measurement results of Examples 2-1 to 2-5, where (a) is the result of color difference and (b) is the result of water loss. FIG. 5B shows the skin condition measurement results of Examples 2-1 to 2-5, (c) is the result of the stratum corneum moisture content, and (d) is the result of elasticity. FIG. 6A shows the skin condition measurement results of Examples 3-1 to 3-5, where (a) is the color difference result and (b) is the water loss amount result. FIG. 6B shows the skin condition measurement results of Examples 3-1 to 3-5, (c) is the result of the stratum corneum moisture content, and (d) is the result of elasticity. FIG. 7A shows the skin condition measurement results of Examples 4-1 to 4-5, where (a) is the color difference result and (b) is the water loss amount result. FIG. 7B shows the skin condition measurement results of Examples 4-1 to 4-5, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 8A shows the skin condition measurement results of Examples 5-1 to 5-5, where (a) is the result of color difference and (b) is the result of water loss. FIG. 8B shows the skin condition measurement results of Examples 5-1 to 5-5, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 10A shows the skin condition measurement results of Examples 6-1 to 6-5, where (a) is the result of color difference and (b) is the result of water loss. FIG. 10B shows the skin condition measurement results of Examples 6-1 to 6-5, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 10A shows the skin condition measurement results of Comparative Example 4 and Examples 7-1 to 7-5, where (a) is the result of color difference and (b) is the result of water loss. FIG. 10B shows the skin condition measurement results of Comparative Example 4 and Examples 7-1 to 7-5, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 11A shows the skin condition measurement results of Comparative Example 5 and Examples 8-1 to 8-5, where (a) is the result of color difference and (b) is the result of water loss. FIG. 11B shows the skin condition measurement results of Comparative Example 5 and Examples 8-1 to 8-5, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 12A shows the skin condition measurement results of Comparative Example 6 and Examples 9-1 to 9-5, where (a) is the result of color difference and (b) is the result of water loss. FIG. 12B shows the skin condition measurement results of Comparative Example 6 and Examples 9-1 to 9-5, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 13A shows the skin condition measurement results of Comparative Example 7 and Examples 10-1 to 10-5, where (a) is the result of color difference and (b) is the result of water loss. FIG. 13B shows the skin condition measurement results of Comparative Example 7 and Examples 10-1 to 10-5, where (c) is the result of stratum corneum moisture content and (d) is the result of elasticity. FIG. 14 shows the results of turnover tests of Comparative Examples 1 to 3 and Examples 1-1 to 1-10. FIG. 15 shows the results of turnover tests of Examples 1-11 to 1-20. FIG. 16 shows the results of turnover tests of Examples 1-21 to 1-30. FIG. 17 shows the results of turnover tests of Examples 1-31 to 1-35. FIG. 18 shows the results of turnover tests of Examples 2-1 to 2-5. FIG. 19 shows the results of turnover tests of Examples 3-1 to 3-5. FIG. 20 shows the results of turnover tests of Examples 4-1 to 4-5. FIG. 21 shows the results of turnover tests of Examples 5-1 to 5-5. FIG. 22 shows the results of turnover tests of Examples 6-1 to 6-5. FIG. 23 shows the results of the turnover test of Comparative Example 4 and Examples 7-1 to 7-5. FIG. 24 shows the results of the turnover test of Comparative Example 5 and Examples 8-1 to 8-5. FIG. 25 shows the results of the turnover test of Comparative Example 6 and Examples 9-1 to 9-5. FIG. 26 shows the results of the turnover test of Comparative Example 7 and Examples 10-1 to 10-5. FIG. 27 shows images of stratum corneum cells observed before and after using the effervescent external preparations for skin of Comparative Example 1, Examples 1-19, Examples 2-3 and 4-3. FIG. 28 shows the evaluation results of cell activating activity of Comparative Examples 8-10 and Examples 11-13.

The external preparation for skin, the kit for external preparation for skin, and the composition for external preparation for skin of the present invention will be described below based on preferred embodiments thereof. The kit for external skin preparation and the composition for external skin preparation of the present invention are each one form of the foaming external preparation for skin of the present invention. The effervescent external preparation for skin of the present invention may contain all of the acidic substance, the carbon dioxide generating substance, the thickening agent and the following specific component in the same agent, or may be divided into a plurality of agents, When divided into a plurality of agents, the four components may be divided in any way. However, the acidic substance, the carbon dioxide generating substance, and the amount of water necessary for generating carbon dioxide are not included in the same agent. The moisture content of the same agent containing an acidic substance and a carbon dioxide generating substance is preferably 15% by mass or less, more preferably 10% by mass or less, and particularly preferably 8% by mass or less.

The carbon dioxide effervescent topical preparation for skin according to the present invention can be used in any form of cosmetics, quasi-drugs, or pharmaceuticals. It is preferably used as a medicated cosmetic), and particularly preferably used as a quasi-drug (medicated cosmetic).

In addition, the carbon dioxide gas effervescent topical preparation for skin according to the present invention can be externally applied to the skin including the scalp and hair, and can be used as packs, hair restorers, facial cleansers, cleansers, lotions, milky lotions, beauty gels, shampoos, It can be used in various forms such as a conditioner. When washed away, it is preferably used for packs, facial cleansers, and cleansers.

First, main components used in the external skin preparation, the kit for external skin preparation, and the composition for external skin preparation of the present invention will be described. Hereinafter, when the term "external skin preparation" is used, the carbon dioxide generating substance, the acidic substance, and the water are brought into contact with each other, and the external skin preparation (e.g., kit for external skin preparation, composition for external skin preparation) before these three are mixed. ), or a carbon dioxide-containing external skin preparation after they are mixed (for example, one obtained by mixing two agents of the kit, or a composition for skin external preparation mixed with a water-containing substance It may also refer to what is obtained by Which one is referred to depends on the context. The components and the like contained in the external preparation for skin, kit and composition of the present invention are collectively described below.

<Acidic substances>
The acidic substance used in the present invention may be either an inorganic acid or an organic acid, and one or more of these may be used.

Inorganic acids include phosphoric acid, potassium dihydrogen phosphate, sodium dihydrogen phosphate, sodium sulfite, potassium sulfite, sodium pyrosulfite, potassium pyrosulfite, acidic sodium hexametaphosphate, acidic potassium hexametaphosphate, acidic sodium pyrophosphate, acidic Potassium pyrophosphate, sulfamic acid and the like.

Examples of organic acids include linear fatty acids such as formic acid, acetic acid, propionic acid, butyric acid, and valeric acid, or salts thereof, oxalic acid, malonic acid, succinic acid, glutaric acid, adipic acid, pimelic acid, fumaric acid, maleic acid, and phthalic acid. Acids, dicarboxylic acids such as isophthalic acid and terephthalic acid or their salts, acidic amino acids such as glutamic acid and aspartic acid or their salts, glycolic acid, malic acid, tartaric acid, citric acid, lactic acid, hydroxyacrylic acid, α-oxybutyric acid, glycerin acids, tartronic acid, salicylic acid, gallic acid, tropic acid, ascorbic acid, gluconic acid and the like, and salts thereof; These 1 type(s) or 2 or more types can be used.

Among them, citric acid, ascorbic acid, malic acid and succinic acid are preferred from the viewpoint of safety and solubility in water.

The content of the acidic substance in the external preparation for skin of the present invention is preferably 0.01 parts by mass or more and 30 parts by weight or less with respect to 100 parts by weight of the external preparation for skin, from the viewpoint of efficiently expressing the cosmetic effect. , more preferably 0.05 parts by mass or more and 20 parts by mass or less. Similarly, the content of the acidic substance in the acid-containing composition of the kit (1) and (2) and the content of the acidic substance in the acid- and base-containing composition of the kit (3) It is preferably 0.01 to 30 parts by mass, more preferably 0.05 to 20 parts by mass, based on 100 parts by mass of the total amount of the two agents. In the case where the external skin preparation of the present invention is a kit containing two or more agents, the amount of the external skin preparation as used herein may be the total amount of each agent in the kit at the time of starting mixing the components of each kit. When an external skin preparation is obtained by mixing a one-part skin external preparation composition and a water-containing substance, the total amount of the composition and the water-containing substance at the start of mixing may be used.

Furthermore, the content of the acidic substance in the acid-containing composition (acid-containing water-containing composition) in the kit of (1) of the present invention is 0.01% by mass in the acid-containing composition (acid-containing water-containing composition). It is preferably at least 30% by mass, more preferably at least 0.05% by mass and not more than 20% by mass.
The content of the acidic substance in the acid-containing composition in the kit of (2) of the present invention is preferably 0.1% by mass or more and 90% by mass or less based on the solid content of the acid-containing composition. More preferably, it is 5% by mass or more and 80% by mass or less.
The content of the acidic substance in the acid- and base-containing composition in the kit of (3) of the present invention is 0.01% by mass or more and 50% by mass or less in the solid content of the acid- and base-containing composition. It is preferably 0.05% by mass or more and 40% by mass or less.
The content of the acidic substance in the external skin preparation composition of the present invention is preferably 0.01% by mass or more and 40% by mass or less, and 0.05% by mass, based on the solid content of the skin external preparation composition. % or more and 30 mass % or less.

<Carbon dioxide generating substance>
The carbon dioxide-generating substance that generates carbon dioxide gas by reacting with the acidic substance used in the present invention may be a substance that generates carbon dioxide by reacting with the acidic substance.

Carbon dioxide generating substances include carbonates such as sodium carbonate, calcium carbonate, potassium carbonate, magnesium carbonate, sodium sesquicarbonate, ammonium hydrogen carbonate, potassium hydrogen carbonate, sodium hydrogen carbonate, lithium hydrogen carbonate, cesium hydrogen carbonate, and magnesium hydrogen carbonate. , bicarbonate such as calcium bicarbonate, and the like, and one or more of these are used. Among these, hydrogencarbonate can be preferably used, and sodium hydrogencarbonate is more preferable in that moderate foaming power can be achieved.

The content of the carbon dioxide-generating substance in the external skin preparation of the present invention is preferably 0.01% by mass or more and 30% by mass or less, more preferably 0.05% by mass or more and 20% by mass, relative to 100 parts by mass of the external skin preparation. % or less. Similarly, the content of the carbon dioxide-generating substance in the base-containing composition of the kits of (1) and (2) and the content of the carbon dioxide-generating substance in the acid- and base-containing composition of the kit of (3) are It is preferably 0.01 to 30 parts by mass, more preferably 0.05 to 20 parts by mass, based on 100 parts by mass of the total amount of the two agents of the kit.

The content of the carbon dioxide generating substance in the base-containing composition in the kit of (1) of the present invention is preferably 0.1% by mass or more and 90% by mass or less based on the solid content of the base-containing composition. More preferably, it is 0.5% by mass or more and 80% by mass or less.
The content of the carbon dioxide generating substance in the base-containing composition (water-containing composition) in the kit (2) of the present invention is preferably 0.01% by mass or more and 30% by mass or less in the base-containing composition. , more preferably 0.05% by mass or more and 20% by mass or less.
The content of the carbon dioxide generating substance in the acid- and base-containing composition in the kit of (3) of the present invention is 0.01% by mass or more and 50% by mass or less in the solid content of the acid- and base-containing composition. is preferable, and more preferably 0.05% by mass or more and 40% by mass or less.
The content of the carbon dioxide generating substance in the composition for external skin preparation of the present invention is preferably 0.01% by mass or more and 40% by mass or less based on the solid content of the composition for external skin preparation. More preferably, it is 05% by mass or more and 30% by mass or less.

<Thickener>
Examples of thickening agents used in the present invention include various substances that can be used in the fields of cosmetics and pharmaceuticals. Examples of thickeners that can be used include synthetic polymers, semi-synthetic polymers, natural polymers, clay minerals, etc. Specifically, synthetic polymers include carboxyvinyl polymer, polyvinyl alcohol, acrylic acid/methacrylic Alkyl acid copolymer, acrylates/alkyl acrylate crosspolymer, polyacrylic acid, polyacrylamide, polyalkylacrylamide/polyacrylamide copolymer, carboxymethylcellulose, cationized cellulose, pluronic, (PEG-240/decyltetradeceth-20/ HDI) copolymers and other hydrophilic synthetic polymers and semisynthetic polymers include, for example, carboxymethylcellulose or salts thereof, methylcellulose, ethylcellulose, propylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose, sulfonated cellulose derivatives, and the like. of cellulose derivatives. Other semi-synthetic polymers include propylene glycol alginate, ethylene glycol alginate, dextrin fatty acid ester, gelatin fatty acid ester, and gelatin fatty acid amide. Natural polymers include polysaccharides and derivatives thereof such as xanthan gum, succinoglycan, carrageenan, guar gum, locust bean gum, celluloses, galactan, gum arabic, tragacanth gum, tamarind gum, agar, agarose, mannan, curdlan, alginic acid or its salts, gum arabic, pectin, quinseed, starch, algecolloid, chondroitin sulfate or its salts, chitosan and its derivatives, nucleic acid or its salts, ribonucleic acid or its salts, casein, collagen, gelatin, albumin, fibroin, Examples thereof include water-soluble proteins such as elastin, keratin and sericin, hyaluronic acid or salts thereof, and mucopolysaccharides such as chondroitin sulfate. Clay minerals include laponite, bentonite, smectite kaolinite, montmorillonite, and the like.

In the present invention, one or more of the above thickeners can be used. It is preferable because it can alleviate the rough feeling of the skin caused by dissolved components and undispersed components. Here, the quality of the solubility in water is defined by the fact that when water at about room temperature is added and stirred, it instantly dissolves in water, or it disperses and dissolves uniformly, and has sufficient viscosity. Alternatively, it can be judged from the fact that lumps do not occur during dispersion.

Preferred thickeners from the viewpoint of solubility and dispersibility in water described above include, for example, carboxyvinyl polymer, carboxymethylcellulose or salts thereof, polyvinyl alcohol, PEG-240/decyltetradeceth-20/ HDI) copolymer, sodium polyacrylate, alkyl acrylate/methacrylate copolymer, xanthan gum, succinoglycan, carrageenan, guar gum, locust bean gum, cellulose derivatives (celluloses), galactan, arabic gum, tragacanth gum, tamarind gum, Agar, mannan, curdlan, alginic acid or its salts, collagen, gelatin, albumin, etc., and particularly preferred are alginic acid or its salts, hydroxyethyl ether, carboxymethylcellulose or its salts, xanthan gum, carrageenan, tamarind gum. , albumin.

The amount of the thickening agent contained in the external skin preparation of the present invention is preferably 0.01 parts by mass or more and 40 parts by mass or less, more preferably 0.1 parts by mass or more and 30 parts by mass, relative to 100 parts by mass of the external skin preparation. Part or less is more preferable. Similarly, the content of the thickener in the acid-containing composition and/or the base-containing composition in the kit of (1) and (2) and the acid- and base-containing composition and/or the water-containing composition in the kit of (3) The content of the thickener in the product is preferably 0.01 parts by mass or more and 40 parts by mass or less, and 0.1 parts by mass or more and 30 parts by mass, based on the total amount of 100 parts by mass of the two agents It is more preferably not more than parts by mass. The amount of thickening agent in the acid-containing composition and/or the base-containing composition referred to herein is the amount when one of them contains the thickening agent, and both contain the thickening agent. The same applies to the amount of thickening agent in the base-containing composition and/or water-containing composition.

The content of the thickening agent in the composition for external skin preparation of the present invention is preferably 0.1% by mass or more and 40% by mass or less based on the solid content of the composition for external skin preparation. More preferably, it is 5% by mass or more and 30% by mass or less.

<Water>
Water that can be used in the present invention can be water that can be used in cosmetics, topical medicines, quasi-drugs, etc., but purified water, distilled water, membrane-filtered water, and ion-exchanged water are preferred.

The amount of water in the topical skin preparation of the present invention (or the amount of water used in the topical skin preparation) is 20 parts by weight per 100 parts by weight of the topical skin preparation from the viewpoint of sufficiently exhibiting the cosmetic effects of the present invention. It is preferably 95 parts by mass or less, more preferably 30 parts by mass or more and 90 parts by mass or less. The amount of water referred to herein is the ratio of water to the total amount of water and the amount of the composition in the case of a composition for external use on skin. Similarly, the content of water in the kits (1) to (3) is preferably 20 parts by mass or more and 95 parts by mass or less with respect to the total amount of 100 parts by mass of the two agents in the kit, and 30 parts by mass. More preferably, the amount is from 90 parts by mass to 90 parts by mass. The amount of water in the kit as used herein is the total amount of water in the two agents, and is the amount of water in the agent when only one of the two agents contains water.

<Polyhydric alcohol>
In the present invention, it is preferable that the external preparation for skin contains a polyhydric alcohol, since a high moisturizing effect can be exhibited by combining it with the specific component described later. Polyhydric alcohols include glycerin, diglycerin, triglycerin, polyglycerin such as tetraglycerin, ethylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, propylene glycol, dipropylene glycol, polyethylene glycol, 1, 3-propanediol, 1,2-pentanediol, sorbitol, polyglycerin derivatives, reducing sugars such as glucose, fructose, glyceraldehyde, lactose, arabinose and maltose, sugar alcohols such as erythritol, maltitol, sorbitol and xylitol These can be used alone or in combination of two or more. Among them, the number of carbon atoms is preferably 2 or more, and more preferably bivalent or more, from the viewpoint of high moisturizing effect.

The amount of the polyhydric alcohol contained in the topical skin preparation of the present invention is 1 part by mass or more and 80 parts by mass with respect to 100 parts by mass of the topical skin preparation from the viewpoint of sufficiently exhibiting the cosmetic effects of the combination with the above-mentioned specific ingredients. parts by mass or less, and more preferably 5 parts by mass or more and 70 parts by mass or less. Similarly, the content of the polyhydric alcohol in the acid-containing composition and/or base-containing composition in the kit of (1) and (2) and the acid- and base-containing composition and/or water-containing composition in the kit of (3) The content of the polyhydric alcohol in the product is preferably 1 part by mass or more and 80 parts by mass or less, and 5 parts by mass or more and 70 parts by mass or less with respect to the total amount of 100 parts by mass of the two agents of the kit. It is even more preferable to have Herein, the amount of polyhydric alcohol in the acid-containing composition and/or base-containing composition is the amount when one of them contains polyhydric alcohol, and both contain polyhydric alcohol. If so, it is the total amount, and the same applies to the amount of polyhydric alcohol in the base-containing composition and/or the water-containing composition.

One of the features of the external preparation for skin of the present invention is that it contains a specific active ingredient (hereinafter also referred to as a specific ingredient). Such ingredients are described in detail below.

<Ascorbic acid glucoside>
Ascorbic acid glucosides include 2-O-α-D-glucopyranosyl-L-ascorbic acid (also called AA-2G, ascorbic acid-2-glucoside, L-ascorbic acid 2-glucoside, etc.). Ascorbic acid glucoside may be isolated from nature or biochemically synthesized. Ascorbic acid glucoside is said to have a melanin production inhibitory effect, and is used in whitening cosmetics and quasi-drugs. The present inventors have found that the effects of ascorbyl glucoside are efficiently exhibited in carbon dioxide effervescent external skin preparations obtained when mixed with an acidic substance, a carbon dioxide generating substance, a thickening agent and water. . Ascorbic acid glucoside may be contained in any of the kits (1) to (3), or may be contained in a single-agent composition for external skin preparation. When ascorbic acid glucoside is contained in a two-agent kit, one of the two agents is gel and the other is solid, one of the two agents is gel and the other is liquid; Both agents may be gel-like agents, one of the two agents may be liquid and the other solid, or both agents may be liquid. Agents containing ascorbic acid glucoside and various specific components described below may be solid, liquid, or gel.

In the present invention, the amount of ascorbyl glucoside in the external preparation for skin is preferably 0.01% by mass or more and 20% by mass or less from the viewpoint of efficiently exhibiting the advantageous effects of ascorbyl glucoside. From this point of view, the amount of ascorbyl glucoside in the external skin preparation is preferably 0.01% by mass or more and 20% by mass or less, more preferably 0.05% by mass or more and 10% by mass or less, and 0.05% by mass or more and 10% by mass or less. It is particularly preferable that the content is 1 part by mass or more and 5 parts by mass or less. Similarly, the content of ascorbic acid glucoside in the acid-containing composition and/or base-containing composition in the kits of (1) and (2) and the acid- and base-containing composition and/or water-containing composition in the kit of (3) The content of ascorbic acid glucoside in the product is preferably 0.01 parts by mass or more and 20 parts by mass or less, and 0.05 parts by mass or more and 10 parts by mass with respect to the total amount of 100 parts by mass of the two agents of the kit. It is more preferably not more than parts by mass, and particularly preferably 0.1% by mass or more and 5% by mass or less. The amount of ascorbic acid glucoside in the acid-containing composition and/or base-containing composition referred to herein is the amount when one of them contains ascorbic acid glucoside, and both contain ascorbic acid glucoside. If so, it is the total amount. The same applies to the amount of ascorbic acid glucoside in the acid- and base-containing composition and/or the water-containing composition.

Ascorbic acid glucoside may be contained in either an acid-containing composition (aqueous composition) or a base-containing composition when it is contained in the kit of (1). When ascorbic acid glucoside is contained in the acid-containing composition of the kit (1), the content is 0.01 in the acid-containing composition from the viewpoint of efficiently exerting the advantageous effects of ascorbic acid glucoside. It is preferably from 0.05 mass % to 10 mass %, and more preferably from 0.05 mass % to 10 mass %. When ascorbic acid glucoside is contained in the base-containing composition of the kit (1), the content is preferably 0.1% by mass or more and 90% by mass or less in the solid content of the base-containing composition. , more preferably 0.5% by mass or more and 80% by mass or less.

Ascorbic acid glucoside, when included in the kit (2), is preferably contained in the acid-containing composition from the viewpoint of efficiently exhibiting the effect of ascorbic acid glucoside in the external preparation for skin. On the other hand, when contained in a base-containing composition, it is difficult to stably exist in the kit. When ascorbic acid glucoside is contained in the acid-containing composition of the kit of (2), its content is preferably 90% by mass or less, and 0.1% by mass or more in the solid content of the acid-containing composition. It is more preferably 80% by mass or less.

Ascorbic acid glucoside, when included in the kit of (3), may be contained in either the acid- and carbonate-containing composition or the water-containing composition. When ascorbic acid glucoside is contained in the acid- and carbonate-containing composition of the kit of (3), its content is 0.1% by mass or more and 50% by mass or less in the solid content of the acid- and carbonate-containing composition. and more preferably 0.5% by mass or more and 40% by mass or less. In addition, when ascorbic acid glucoside is contained in the water-containing composition of the kit of (3), the content is preferably 0.01% by mass or more and 20% by mass or less in the water-containing composition, and 0.05% by mass % or more and 10% by mass or less.

The content of ascorbic acid glucoside in the composition for external skin preparation of the present invention is preferably 0.1% by mass or more and 30% by mass or less, based on the solid content of the composition for external skin preparation, and 0.5%. More preferably, it is at least 20% by mass and no more than 20% by mass.

<Another specific ingredient>
Even when the external skin preparation, the kit for external skin preparation, and the composition for external skin preparation of the present invention contain a specific active ingredient other than ascorbic acid glucoside, they exhibit effects such as cosmetic effects due to the inclusion of the ingredient. can do. Such ingredients are listed below. The preferred ratio of the specific component contained in the kit for external skin preparation of the present invention to the total amount of the two agents is the same as the preferred quantity of the specific component in the external skin preparation shown below. In addition, the preferred content of the specific component in the external skin preparation composition is the same as the preferred content of the specific component in the solid content of the external skin preparation shown below.

Allantoin is _a compound with _the composition _C4H6N4O3 and is the diureide of glyoxylic _acid . Also known as 5-ureidohydantoin and glyoxydiureide, it is known as an anti-inflammatory agent. When the external skin preparation contains allantoin, the amount thereof is preferably 0.001% by mass or more and 20% by mass or less, more preferably 0.01% by mass or more and 10% by mass or less, in the external skin preparation. The amount of allantoin is preferably 0.01% by mass or more and 30% by mass or less, more preferably 0.05% by mass or more and 20% by mass or less, based on the solid content of the external preparation for skin.

When allantoin is contained in the acid-containing composition of the kit (1), the content is preferably 0.01% by mass or more and 20% by mass or less in the acid-containing composition, and 0.05 More preferably, the content is not less than 10% by mass and not more than 10% by mass. When allantoin is contained in the base-containing composition of the kit (1), the content is preferably 0.01% by mass or more and 30% by mass or less in the solid content of the base-containing composition. More preferably, it is 05% by mass or more and 20% by mass or less.

When allantoin is contained in the acid-containing composition of the kit (2), the content is preferably 0.01% by mass or more and 30% by mass or less in the solid content of the acid-containing composition. It is more preferably 05% by mass or more and 20% by mass or less. When allantoin is contained in the base-containing composition of the kit (2), the content thereof is preferably 0.01% by mass or more and 20% by mass or less in the base-containing composition, and 0.05% by mass. % or more and 10% by mass or less.

When allantoin is contained in the acid- and carbonate-containing composition of the kit of (3), its content is 0.01% by mass or more and 30% by mass or less in the solid content of the acid- and carbonate-containing composition. 0.05% by mass or more and 20% by mass or less is more preferable. When allantoin is contained in the water-containing composition of the kit (3), the content is preferably 0.01% by mass or more and 20% by mass or less in the water-containing composition, and 0.05% by mass or more. It is more preferably 10% by mass or less.

Tranexamic acid, also called trans-4-(aminomethyl)cyclohexanecarboxylic acid, is known as an anti-inflammatory agent and whitening agent. When the external skin preparation contains tranexamic acid, the amount thereof is preferably 0.01% by mass or more and 20% by mass or less, more preferably 0.1% by mass or more and 10% by mass or less, in the external skin preparation. The amount of tranexamic acid is preferably 0.1% by mass or more and 80% by mass or less, more preferably 1% by mass or more and 70% by mass or less, of the solid content of the external preparation for skin.

When tranexamic acid is contained in the acid-containing composition of the kit (1), the content is preferably 0.1% by mass or more and 60% by mass or less in the acid-containing composition, and 1 mass % or more and 50 mass % or less. When tranexamic acid is contained in the base-containing composition of the kit (1), the content is preferably 1% by mass or more and 90% by mass or less in the solid content of the base-containing composition, and 5% by mass. % or more and 80 mass % or less.

When tranexamic acid is contained in the acid-containing composition of the kit (2), its content is preferably 1% by mass or more and 90% by mass or less, and 5% by mass of the solid content of the acid-containing composition. It is more preferable that the content is not less than 80% by mass and not more than 80% by mass. When tranexamic acid is contained in the base-containing composition of the kit (2), the content is preferably 0.1% by mass or more and 60% by mass or less in the base-containing composition, and 1% by mass. It is more preferable that the content is at least 50% by mass or less.

When tranexamic acid is contained in the acid- and carbonate-containing composition of the kit (3), its content is 0.1% by mass or more and 80% by mass or less in the solid content of the acid- and carbonate-containing composition. It is preferably 1% by mass or more and 75% by mass or less. When tranexamic acid is contained in the water-containing composition of the kit (3), the content is preferably 0.01% by mass or more and 30% by mass or less in the water-containing composition, and 0.1% by mass. It is more preferable that the content is at least 20% by mass or less.

d-Camphor has a chemical name of (1R,4R)-1,7,7-Trimethylbicyclo[2.2.1]heptan-2-one, and is one of the bicyclic monoterpene ketones. It is obtained by steam distillation of the wood pieces contained in. When the topical skin preparation contains d-camphor, its amount is preferably 0.001% by mass or more and 10% by mass or less in the topical skin preparation. The amount of d-camphor is preferably 0.01% by mass or more and 10% by mass or less based on the solid content of the external preparation for skin.

Salicylic acid is _a compound with the chemical _{formula C7H6O3} . When the external skin preparation contains salicylic acid, the amount thereof is preferably 0.0001% by mass or more and 10% by mass or less in the external skin preparation. Moreover, the amount of salicylic acid is preferably 0.001% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Biotin is called D-[(+)-cis-hexahydro-2-oxo-1H-thieno-(3,4)-imidazole-4-valeric acid] and is a kind of water-soluble vitamin classified as vitamin B group. is. When the external skin preparation contains biotin, the amount thereof is preferably 0.0001% by mass or more and 1% by mass or less in the external skin preparation. Moreover, the amount of biotin is preferably 0.0001% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Resorcinol is a compound represented by the chemical formula C ₆ H ₄ (OH) ₂ and corresponds to 1,3-dihydroxybenzene. When the external skin preparation contains resorcinol, the amount thereof is preferably 0.001% by mass or more and 10% by mass or less in the external skin preparation. Moreover, the amount of resorcin is preferably 0.01% by mass or more and 20% by mass or less in the solid content of the external preparation for skin.

Trichlorocarbanilide is also called triclocarban or 3,4,4'-trichlorocarbanilide. When the external skin preparation contains trichlorocarbanilide, the amount thereof is preferably 0.001% by mass or more and 10% by mass or less in the external skin preparation. Further, trichlorocarbanilide is preferably 0.01% by mass or more and 20% by mass or less in the solid content of the external preparation for skin.

Triclosan is also called 5-chloro-2-(2',4'-dichlorophenoxy)phenol. When the topical skin preparation contains triclosan, its amount is preferably 0.0001% by mass or more and 10% by mass or less in the topical skin preparation. Moreover, the amount of triclosan is preferably 0.001% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Benzethonium chloride is represented by the chemical formula C ₂₇ H ₄₂ ClNO ₂ and is a kind of cationic surfactant. When the external skin preparation contains benzethonium chloride, the amount thereof is preferably 0.0001% by mass or more and 10% by mass or less in the external skin preparation. The amount of benzethonium chloride is preferably 0.001% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Sulfur is an element represented by the chemical formula S. When the external preparation for skin contains sulfur, the amount thereof is preferably 0.001% by mass or more and 20% by mass or less in the external preparation for skin. Moreover, the amount of sulfur is preferably 0.01% by mass or more and 20% by mass or less in the solid content of the external preparation for skin.

Photosensitizer No. 201 is also called pionin. When the external preparation for skin contains Photosensitizer No. 201, the amount thereof is preferably 0.0001% by mass or more and 5% by weight or less in the external preparation for skin. Moreover, the amount of pionin is preferably 0.0005% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Pyridoxine is a water-soluble compound called 4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol, or vitamin B6. When the external skin preparation contains pyridoxine, the amount thereof is preferably 0.0001% by mass or more and 5% by mass or less in the external skin preparation. Moreover, the amount of pyridoxine is preferably 0.0005% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Pyridoxine hydrochloride is a hydrochloride of pyridoxine, and is also called 5-Hydroxy-6-methylpyridine-3,4-dimethylol monohydrochloride. When the external preparation for skin contains pyridoxine hydrochloride, the amount thereof is preferably 0.001% by mass or more and 10% by mass or less in the external preparation for skin. Moreover, the amount of pyridoxine hydrochloride is preferably 0.01% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Zinc oxide is represented by the chemical formula ZnO. When the external skin preparation contains zinc oxide, the amount thereof is preferably 0.01% by mass or more and 20% by mass or less in the external skin preparation. Moreover, 0.05 mass % or more and 20 mass % or less is preferable in the solid content of the external preparation for skin.

Arbutins include β-arbutin and α-arbutin, and β-arbutin called 4-(β-D-glucopyranosyloxy)phenol is common. When the external skin preparation contains arbutin, the amount thereof is preferably 0.01% by mass or more and 20% by mass or less, more preferably 0.1% by mass or more and 10% by mass or less, in the external skin preparation. In addition, the amount of arbutin

It is preferably 0.1% by mass or more and 50% by mass or less, more preferably 1% by mass or more and 40% by mass or less in the solid content of the external preparation for skin.

When arbutin is contained in the acid-containing composition of the kit (1), the content is preferably 0.1% by mass or more and 30% by mass or less in the acid-containing composition, and 1% by mass. It is more preferable that the content is at least 20% by mass or less. When arbutin is contained in the base-containing composition of the kit (1), the content is preferably 1% by mass or more and 90% by mass or less in the solid content of the base-containing composition, and 5% by mass. It is more preferable that the content is at least 85% by mass or less.

When arbutin is contained in the acid-containing composition of the kit (2), the content is preferably 1% by mass or more and 90% by mass or less, and 5% by mass or more in the solid content of the acid-containing composition. It is more preferably 85% by mass or less. When arbutin is contained in the base-containing composition of the kit (2), the content is preferably 0.1% by mass or more and 30% by mass or less in the base-containing composition, and 1% by mass or more. It is more preferably 20% by mass or less.

When arbutin is contained in the acid- and carbonate-containing composition of the kit (3), its content is 1% by mass or more and 80% by mass or less in the solid content of the acid- and carbonate-containing composition. It is preferably 5% by mass or more and 70% by mass or less. When arbutin is contained in the water-containing composition of the kit (3), the content is preferably 0.1% by mass or more and 30% by mass or less in the water-containing composition, and 1% by mass or more and 20% by mass. % or less.

Magnesium L-ascorbyl phosphate is also called magnesium L-ascorbate-2-phosphate. When the external skin preparation contains magnesium L-ascorbate-2-phosphate, the amount thereof is preferably 0.01% by mass or more and 20% by mass or less in the external skin preparation. Moreover, 0.05 mass % or more and 20 mass % or less is preferable in the solid content of the external preparation for skin.

Ascorbyl dipalmitate is also called L-ascorbic acid 2,6-dipalmitate. When the external skin preparation contains ascorbyl palmitate, the amount thereof is preferably 0.01% by mass or more and 20% by mass or less in the external skin preparation. Moreover, 0.05 mass % or more and 20 mass % or less is preferable in the solid content of the external preparation for skin.

Glycyrrhizic acid includes β-glycyrrhizic acid. Examples of glycyrrhizic acid derivatives include dipotassium glycyrrhizinate (sometimes referred to as "dipotassium glycyrrhizinate"), monoammonium glycyrrhizinate, and stearyl glycyrrhizinate. Monoammonium glycyrrhizinate may be either an anhydride or a hydrate. When the topical skin preparation contains these glycyrrhizic acid and derivatives thereof, the amount of one kind and the total amount thereof in the topical skin preparation is preferably 0.0001% by mass or more and 10% by mass or less, and 0.001% by mass or more. 1 mass % or less is more preferable. The content of glycyrrhizic acid and its derivatives is preferably 0.001% by mass or more and 20% by mass or less, more preferably 0.005% by mass or more and 10% by mass or less, in the solid content of the external skin preparation.

When glycyrrhizic acid and its derivatives are contained in the acid-containing composition of the kit (1), the content thereof is preferably 0.001% by mass or more and 10% by mass or less in the acid-containing composition. , 0.005% by mass or more and 5% by mass or less. In addition, when glycyrrhizic acid and its derivatives are contained in the base-containing composition of the kit (1), the content thereof is 0.01% by mass or more and 20% by mass or less in the solid content of the base-containing composition. is preferable, and more preferably 0.05% by mass or more and 10% by mass or less.

When glycyrrhizic acid and its derivatives are contained in the acid-containing composition of the kit of (2), the content is 0.01% by mass or more and 20% by mass or less in the solid content of the acid-containing composition. It is preferably 0.05% by mass or more and 10% by mass or less. When glycyrrhizic acid and its derivatives are contained in the base-containing composition of the kit (2), the content is preferably 0.001% by mass or more and 10% by mass or less in the base-containing composition, It is more preferably 0.005% by mass or more and 5% by mass or less.

When glycyrrhizic acid and its derivatives are contained in the acid- and carbonate-containing composition of the kit of (3), the content thereof is 0.01% by mass or more and 20% by mass of the solid content of the acid- and carbonate-containing composition. % or less, more preferably 0.05 mass % or more and 10 mass % or less. When glycyrrhizic acid and its derivatives are contained in the water-containing composition of the kit (3), the content thereof is preferably 0.001% by mass or more and 10% by mass or less in the water-containing composition. 050% by mass or more and 5% by mass or less is more preferable.

Each of the above preferred amounts of glycyrrhizic acid and its derivatives may be the amount of one kind of glycyrrhizic acid and its derivatives, or the total amount of glycyrrhizic acid and its derivatives.

Retinol is also called vitamin A. When the external skin preparation contains retinol, the amount thereof is preferably 0.00001% by mass or more and 20% by mass or less in the external skin preparation. Moreover, it is preferably 0.00005% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Retinol palmitate is also called vitamin A palmitate. When the external skin preparation contains retinol palmitate, the amount thereof is preferably 0.00001% by mass or more and 20% by mass or less in the external skin preparation. Moreover, it is preferably 0.00005% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Estradiol has the common name estra-1.3.5(10)triene-3.17β-diol. Ethynylestradiol has the common name 17-ethynylestra-1,3,5(10)-triene-3β,17β-diol. When the external skin preparation contains these estradiols, the amount of one kind and the total amount are preferably 0.00001% by mass or more and 5% by mass or less in the external skin preparation. Moreover, it is preferably 0.00005% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Both dl-α tocopherol acetate and dl-α tocopherol nicotinate are known as vitamin E derivatives. When the external skin preparation contains dl-α tocopherol acetate and/or dl-α tocopherol nicotinate, the amount of one kind and the total amount are preferably 0.0001% by mass or more and 10% by mass or less in the external skin preparation. . Moreover, it is preferably 0.001% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

D-pantothenyl alcohol is also called (R)-2,4-dihydroxy-N-(3-hydroxypropyl)-3,3-dimethylbutanamide. When the external skin preparation contains D-pantothenyl alcohol, the amount thereof is preferably 0.01% by mass or more and 20% by mass or less in the external skin preparation. Moreover, 0.05 mass % or more and 20 mass % or less is preferable in the solid content of the external preparation for skin.

Pantothenyl ethyl ether is also called (2R)-N-(3-ethoxypropyl)-2,4-dihydroxy-3,3-dimethylbutanamide. When the external skin preparation contains pantothenyl ethyl ether, the amount thereof is preferably 0.001% by mass or more and 10% by mass or less in the external skin preparation. Moreover, it is preferably 0.005% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Phenol has a rational formula of C ₆ H ₅ OH and has a structure in which one hydrogen atom of benzene is substituted with a hydroxyl group. When the external preparation for skin contains phenol, the amount thereof is preferably 0.001% by mass or more and 10% by mass or less in the external preparation for skin. Moreover, it is preferably 0.005% by mass or more and 10% by mass or less in the solid content of the external preparation for skin.

Placenta is a collective term for growth factors and other nutrients that promote cell division in the placenta of animals, preferably pigs, horses, cows, sheep, and humans, nutrients in the ovarian membrane of fish, and nutrients in plant germ. The placenta to be used in the present invention is not particularly limited as long as it can be used as cosmetics or quasi-drugs. In the present invention, commercially available water-soluble extracts and powders can be used. When the external preparation for skin contains placenta, its amount is preferably 0.01% by mass or more and 20% by mass or less in the external preparation for skin. Moreover, 0.05 mass % or more and 20 mass % or less is preferable in the solid content of the external preparation for skin.

Among these specific ingredients, allantoin, dipotassium glycyrrhizinate, stearyl glycyrrhizinate, d-camphor, salicylic acid, arbutin, and placenta are preferably contained in a two-agent skin external preparation kit in the present invention, particularly It is preferably contained in the kit of (1) or (2). Needless to say, the specific components including these may be contained in a single-agent composition for external skin preparation, or may be contained in a two-agent skin external preparation kit. In particular, it is preferable that the specific component is used in a two-agent kit from the viewpoint of increasing the moisture content of the horny layer after use and exhibiting an excellent turnover effect. In addition, the effect of improving the skin condition and the effect of improving the feeling of use of the present invention are not limited by the type of thickener, but the desired effect of these can be further enhanced by the type of thickener combined with a specific component. Even higher is possible.

For each specific component, one of the two agents is gel-like and the other is solid, one of the two agents is gel-like and the other is liquid, both of the two agents are gel-like, One of the two agents may be liquid and the other solid, or both agents may be liquid. For dl-α tocopherol acetate, one of the two agents is gel and the other is liquid, both agents are gel, and one of the two agents is liquid and the other is solid. It is preferably used for an agent, or an agent in which both agents are liquid. Furthermore, with respect to the placenta, one of the two agents is gel-like and the other is solid, one of the two agents is gel-like and the other is liquid, both of the two agents are gel-like, or It is preferable that both agents are used in a liquid agent.

In addition, in the kits (1) to (3), estradiol, ethinyl estradiol, dl-α-tocopherol nicotinate, dl-α-tocopherol nicotinate, D-pantothenyl alcohol, pantothenyl ethyl ether, phenol, and placenta contain water. Incorporation in the composition ((1) the acid-containing composition of the kit, (2) the base-containing composition of the kit, (3) the water-containing composition of the kit) is preferable in order to exhibit its effect. These specific ingredients (estradiol, ethinyl estradiol, dl-α-tocopherol nicotinate, dl-α-tocopherol nicotinate, D-pantothenyl alcohol, pantothenyl alcohol) contained in these water-containing compositions in the kits of (1) to (3) Tenyl ethyl ether, phenol, placenta) are preferably in the same preferred proportions as the above-described preferred proportions of these specific ingredients in the external preparation for skin.

Allantoin, glycyrrhizic acid and its derivatives, tranexamic acid, d-camphor, salicylic acid, biotin, resorcinol, trichlorocarbanilide, triclosan, benzonium chloride, sulfur, photosensitizer No. 201, pyridoxines, zinc oxide, arbutin, retinol, palmitic acid For retinol, the effect is obtained when it is included in (1) the base-containing composition or acid-containing composition of the kit, (2) the acid-containing composition of the kit, and (3) the acid- and base-containing composition of the kit. It is preferable for performance. These specific ingredients (allantoin, glycyrrhizic acid and its derivatives, tranexamic acid, d-camphor, salicylic acid, biotin, resorcinol, trichlorocarbanilide, triclosan, benzonium chloride, sulfur, photosensitizer No. 201, pyridoxines, zinc oxide, arbutin, retinol, and retinol palmitate), the preferred ratio to the total amount of the two agents is based on 100 parts by mass of the external skin preparation described above. It is preferred that the proportions be similar to the preferred proportions of these specific components.

<Other ingredients>
The external preparation for skin of the present invention contains other active ingredients, preservatives, pH adjusters, oils and fats, perfumes, coloring agents, antioxidants, antibacterial and antifungal agents, alcohols, nonionic surfactants, anionic surfactants, and cationic surfactants. It is possible to use one or more of the components usually used in external preparations for skin, such as active agents, surfactants such as amphoteric surfactants, inorganic salts, lubricants, and solvents. These are contained in the composition for external skin preparation of the present invention. In addition, in the kit of the present invention, the component to be added can be used for either the first agent or the second agent, or may be used for either agent.

In the external skin preparation, kit, and composition for external skin preparation of the present invention, other ingredients (water, acidic substances, carbon dioxide generating substances, thickeners, polyhydric alcohols, and ingredients other than the above-mentioned specific ingredients) are contained. The total amount is preferably 30% by mass or less, more preferably 25% by mass or less.

<Dosage form and amount of ingredients of the kit>
The kits (1) to (3) are further described in detail. In the kits (1) to (3), the water-containing composition of each kit (acid-containing composition of the kit of (1), base-containing composition of the kit of (2), water-containing composition of the kit of (3)) As a dosage form of , a gel form or a liquid form can be mentioned. In addition, as the dosage form of the other agent of each kit ((1) kit base-containing composition, (2) kit acid-containing composition, (3) kit acid- and base-containing composition)) , solid, liquid, gel, etc. may be used. In the case of a solid form, it is preferably in the form of granules, fine granules, or powder from the viewpoints of ease of mixing with the other agent and production costs.

<Water-containing composition of kit (1) to (3)>
The preferred amounts of the carbon dioxide generating substance, the acidic substance and the specific component in the water-containing composition of the kits (1) to (3) are as described above. In addition, the proportion of water in the water-containing composition of the kits (1) to (3) is preferably 20% by mass or more, particularly 30% by mass or more, from the viewpoint of miscibility with the other agent of each kit, etc. It is preferable that the content is 95% by mass or less, particularly 90% by mass or less, from the viewpoint of containing other components and enhancing the cosmetic effects of the external preparation for skin.

In the case where the water-containing composition of the kit of (1) to (3) contains a polyhydric alcohol, the proportion of the polyhydric alcohol in the water-containing composition is 1% by mass or more, particularly 10% by mass or more. It is preferable from the viewpoint of enhancing the moisturizing effect by combination with a specific ingredient, etc., and it is 90% by mass or less, particularly 80% by mass or less, particularly 70% by mass or less containing other ingredients for cosmetic use as a skin external preparation. It is preferable from the point of enhancing the effect.

In addition, each water-containing composition (1) to (2) preferably contains the above-described acidic substance, carbon dioxide-generating substance, and specific component in the above-described amounts. In addition, to each water-containing composition of (1) to (3), moisturizing agents other than polyhydric alcohols, surfactants, pH adjusters, volatile alcohols, oils and fats, fragrances, extracts of animals and plants, etc. may be added. can be done. For example, moisturizing agents other than polyhydric alcohols include polyoxyalkylene alkyl glucosides, sodium lactate, sodium pyrrolidonecarboxylate, almonds, avocados, althea, aloe, marshmallow, ononis, oat, licorice, quince seed, clara (cucumber), Gardenia, Grapefruit, Watercress, Gentian, Gennoshoko, Burdock, Wheat, Rhinoceros, Cactus, Soapwort, Hawthorn, Rhododendron, Spiraea, Ginger, Mallow, Mulberry, Thymus, Cordyceps, Houttuynia cordata, Mentha, Hatomugi, Hamamelis, Rose, Cypress, Coltsfoot , grapes, prunes, luffa, bodaiju, hops, pine, quince, horse chestnut, melissa, cornflowers, lilies, limes, lavender, apples, rice and rice bran, black currant, Ibukitoranoo, rose rose, Eleuthero, seaweed, etc. and one or more of these can be used.

The liquid agent preferably has a viscosity at 25° C. of less than 5000 mPa·s, more preferably less than 4000 mPa·s. The gel-like agent preferably has a viscosity of 5000 Pa·s or more at 25°C. This viscosity is measured, for example, with a viscometer (RVT model manufactured by Brookfield). Also, the amount of the thickening agent in the liquid agent is preferably less than 0.05% by mass, more preferably less than 0.01% by mass, and particularly preferably less than 0.005% by mass. On the other hand, the amount of the thickening agent in the gel-like agent is preferably 0.01% by mass or more, more preferably 0.05% by mass or more.

<(1) kit base-containing composition, (2) kit acid-containing composition, (3) kit acid- and base-containing composition>
Preferred amounts of the acid, carbon dioxide generating substance, and specific component in these compositions are as described above. When these compositions are in solid form, the amount and content of the thickening agent is preferably 0.01% by mass or more and 20% by mass or less, particularly 0% by mass or less, in these compositions when the other agent is in gel form. .05% by mass or more and 15% by mass or less is preferable from the viewpoint of good foaming, carbon dioxide retention, and exertion of the effect of the specific component. .01% by mass or more and 30% by mass or less, particularly 0.05% by mass or more and 25% by mass or less, from the viewpoint of ease of mixing with the liquid agent, foamability, carbon dioxide retention, and exertion of the effect of the specific component. preferable.

In addition, as described above, the base-containing composition of the kit (1), the acid-containing composition of the kit (2), and the acid- and base-containing composition of the kit (3) may be gel or liquid. good. In that case, the preferred amount of the thickening agent in these compositions is the same as the preferred amount of the liquid or gel thickening agent in the water-containing composition of the kit (1) to (3) described above. Also, when the base-containing composition of the kit (1), the acid-containing composition of the kit (2), and the acid- and base-containing composition of the kit (3) are gel or liquid, in these compositions, The water content is preferably 7% by mass or more, particularly 10% by mass or more, particularly 20% by mass or more, from the viewpoint of foamability and bubble sustainability, and is 95% by mass or less, particularly 90% by mass or less. is preferable from the viewpoint of containing other ingredients to enhance the cosmetic effect of the external preparation for skin.

When the base-containing composition of the kit of (1), the acid-containing composition of the kit of (2), and the acid- and base-containing composition of the kit of (3) contain a polyhydric alcohol, these compositions When is gel or liquid, the content of polyhydric alcohol in these compositions is preferably 1% by mass or more, particularly 10% by mass or more from the viewpoint of ease of use, etc., and 90% by mass %, particularly 80% by mass or less, particularly 70% by mass or less, from the viewpoint of enhancing the cosmetic effects of the external preparation for skin by containing other ingredients. When the base-containing composition of the kit of (1), the acid-containing composition of the kit of (2), and the acid- and base-containing composition of the kit of (3) are in gel or liquid form, others contained in these compositions Ingredients include surfactants, preservatives, preservatives, pH adjusters, fragrances, colorants, antioxidants, antibacterial and antifungal agents, and the like.

When the base-containing composition of the kit of (1), the acid-containing composition of the kit of (2), and the acid- and base-containing composition of the kit of (3) are in solid form, when granulated into granules, the following method.

When granules are obtained by granulating a mixture of an acidic substance and/or a carbon dioxide-generating substance with other ingredients, the content of the other ingredients is not particularly limited, but should be less than 95% by mass in the granules. is preferred. If the content of other components exceeds 95% by mass, it is not preferable because the foamability is lowered.
The method for producing the above powder or granules is not limited to this embodiment, and includes dry crushing and granulation methods, wet crushing and granulation methods, fluid bed granulation methods, high-speed stirring granulation methods, extrusion granulation methods, and the like. It can be manufactured according to a conventional method.

For example, in the production of granules, a matrix base material having functions such as granule molding may be used. When a low melting point compound is used as the matrix base, the carbon dioxide generating substance is added to the low melting point compound melted by heating in a container such as a beaker, and the mixture is thoroughly stirred and mixed. Appropriate additives may be added to this as required. This is further stirred while being gradually cooled to room temperature, and left to stand until it solidifies. If it solidifies to some extent, it may be rapidly cooled in a refrigerator or the like.

Alternatively, for example, the above materials may be charged into a fluidized bed granulator, mixed for several minutes with an air flow, and then granulated by spraying water.

When a low melting point compound is not used as the matrix base, the granulating agent is dissolved or dispersed in a suitable solvent such as water or ethanol in a container such as a beaker, and the carbon dioxide generating substance is dissolved or dispersed therein. After the mixture is sufficiently mixed in the oven, the solvent is removed by heating in an oven or the like, and the mixture is dried. When completely solidified, the mixture is pulverized and sieved to make the grains of uniform size, and then granulated.

The shape of the acidic substance and/or the carbon dioxide gas-generating substance may be irregular, planar, polyhedral, spherical, drop-shaped, fibrous, cylindrical, fine powder, or the like, without particular limitation. Moreover, as the particle size, a wide range of particles can be used without any particular limitation. In particular, from the viewpoint of ease of handling and ease of mixing with the viscous composition, particles having a particle size distribution of about 1,000 μm or less are more preferable. The particle size distribution in the present invention can be determined by a conventional laser diffraction/scattering method.

The acidic substance and/or carbon dioxide generating substance may be blended as they are with the components contained in the same agent, but are blended so as not to physically contact with other components in the same agent and coexist. Also good. In this case, for example, one or both of the acidic substance and/or the carbon dioxide-generating substance and other components may be provided with a coating layer that wraps them, or the carbon dioxide-generating substance and the other components may be coated. Either or both may be encapsulated. In addition, the acidic substance and/or the carbon dioxide generating substance are compressed by sandwiching a layer not containing the carbon dioxide generating substance, the acidic substance and other components between each component or another component so that they do not come into direct contact with each other. It can be molded. Known methods can be used as means for coexisting the acidic substance and/or the carbon dioxide generating substance and other components in the same agent.

In addition, when the kits of (1) to (3) are water-containing compositions, the mixing ratio (mass ratio) of the two is preferably 1:0.1 or more and 20 or less, and 1:0.2 or more and 10 The following are more preferable. When the kit of (1) to (3) consists of a water-containing composition and a composition that does not substantially contain water (for example, a composition in which the proportion of water is 5% by mass or less), the mixing ratio (mass ratio ), the ratio of the former to the latter is preferably 1:0.01 to 30, more preferably 1:0.05 to 20.

The amount of each of the acidic substance, the carbon dioxide-generating substance, the thickening agent, the water and the specific component used in the external preparation for skin of the present invention (or used for mixing to obtain the carbon dioxide-containing external preparation for skin) is , 0.1 to 15% by mass of acidic substances, 0.1 to 20% by mass of carbon dioxide generating substances, 0.1 to 20% by mass of thickeners, 40 to 99.6% by mass of water, and specific components 0.01 to 30% by mass is preferred. When the external preparation for skin contains a polyhydric alcohol, an acidic substance, a carbon dioxide generating substance, a thickener, water, a specific component and The amount of each polyhydric alcohol used is 0.1 to 10% by mass for the acidic substance, 0.1 to 10% by mass for the carbon dioxide generating substance, and 0.1 to 15% by mass for the thickener, based on the total amount. , 30 to 97% by mass of water, 0.01 to 20% by mass of specific components, and 0.05 to 50% by mass of polyhydric alcohol. The amount of these components can be obtained as the amount in the kit for obtaining the external skin preparation or the composition for external skin preparation, or the amount of water mixed with the composition.

<Usage form of composition for external preparation for skin>
The composition for external use on the skin of the present invention may be in any form such as solid, liquid or gel. The shape is preferred, and among solid forms, granules, fine granules and powder are more preferred. A composition for external use on the skin is foamed by mixing it with a water-containing substance on the palm of the hand or in a container. Examples of water-containing substances that can be used include liquids containing water that are commonly used in cosmetics, pharmaceuticals, and the like, and that are used in general households. The liquid containing water may be water itself. Specifically, in addition to tap water, distilled water, membrane filtered water, ion-exchanged water, deep sea water, sake, wine and other alcoholic beverages, soy milk, drinking yogurt, acerola juice, sports drinks, carbonated water and other beverages, rice Notogijiru and the like. These may be used alone or in combination of two or more.

The amount of water-containing substance to be used is not particularly limited and can be used in a wide range. It is more preferable to add an amount. By adding more than one volume of the liquid, the external preparation for skin can be rapidly dissolved, and a sufficient amount of carbon dioxide gas can be generated. On the other hand, by adding the liquid within 10 times the amount, it is possible to prevent dripping due to a decrease in the viscosity of the external preparation for skin.

The temperature of the water-containing substance to be used is not particularly limited, and a wide range can be used. is particularly preferred. As for the usable temperature range, it is preferable to use water at about room temperature or tap water from the viewpoint of feeling during application and convenience for the user.

When the composition for external use on the skin or the component of the kit is a solid substance, the method for preserving the kit is not particularly limited as long as it is preserved in a state where it is protected from moisture and does not come into contact with it. The shape of the storage container to be used can be appropriately selected according to the purpose, and examples thereof include cup-like, tube-like, bag-like, bottle-like, stick-like, pump-like, jar-like, and canned-like shapes. Materials constituting the storage container may be selected from, for example, plastic, glass, aluminum, paper, various polymers, and the like, but not limited to these.

Specific examples of containers include storage containers such as aluminum sticks and aluminum bags laminated with polyethylene terephthalate on the inner surface, standing pouches with zippers, and polyethylene terephthalate on the inner surface in terms of airtightness, storage stability of contents, and manufacturing cost. A storage container or the like made of polyethylene terephthalate, the lid of which is heat-sealed with an aluminum film or the like laminated with , is preferred.

<Uses of external preparations for skin>
The skin external preparation of the present invention promotes an increase in skin blood flow due to the action of carbon dioxide gas. The external preparation for skin of the present invention has excellent skin-improving effects such as whitening the skin color, increasing the water content of the skin, reducing the amount of water evaporation, and improving the elasticity of the skin. In addition, the skin external preparation of the present invention has a feeling of use (ease of mixing, smoothness of foam, fineness of foam, uniformity of foam, ease of application, difficulty of dripping, elasticity of foam, carbonic acid feeling, and The degree of persistence, etc.) and the feeling after use (skin moistness, elasticity, brightness, redness, smoothness, smoothness, tightness, etc.). In addition, the external preparation for skin of the present invention promotes skin metabolism (turnover). Furthermore, the topical preparation for skin of the present invention has an activating effect on skin cells by containing a specific active ingredient in carbon dioxide foaming topical skin preparations, and can be used as a skin cell activator. In addition, the external preparation for skin of the present invention has activation of skin metabolism and promotion of ATP production due to its cell activation action. Taking advantage of these effects, the external preparation for skin of the present invention can be used for whitening, improving skin quality, improving freckles, rejuvenating skin, tightening skin, thinning parts, cleaning skin, conditioning skin, and improving skin texture. , to keep the skin healthy, to prevent rough skin, to tighten the skin, to give moisture to the skin, to replenish and retain moisture and oil in the skin, to keep the skin flexible, to protect the skin, to prevent the skin from drying out, the skin Not only cosmetics aimed at softening the skin, giving the skin firmness, giving the skin luster, smoothing the skin, preventing spots and freckles caused by sunburn, and making fine wrinkles caused by dryness inconspicuous. Prevents rough skin, rough skin, heat rash, frostbite, cracks, chapped skin, acne, oily skin, razor burn, spots and freckles caused by sunburn, prevents hot flashes after sunburn and snow burn, tightens skin Any pharmaceuticals such as quasi-drugs, drugs, etc. for the purpose of cleaning the skin, conditioning the skin, keeping the skin healthy, moisturizing the skin, protecting the skin, preventing the skin from drying out, etc. can also be suitably used. The external preparation for skin of the present invention is preferably used as cosmetics and quasi-drugs, and is preferably used as cosmetics such as emulsions, creams, packs and peeling agents, and medicinal cosmetics. Among them, it is particularly preferable to use it as a pack agent because it gives a good feeling when used and easily obtains a high skin condition improving effect.

The present invention also provides a carbon dioxide effervescent external preparation for skin obtained by mixing an acidic substance, a carbon dioxide generating substance that generates carbon dioxide gas by reacting with the acidic substance, a thickening agent and water, and further comprising: A carbon dioxide effervescent external preparation for skin (carbon dioxide-containing composition) containing the specific component is provided.

EXAMPLES The present invention will be described in more detail below with reference to Examples, but the scope of the present invention is not limited to these Examples. In each of the following examples and comparative examples, commercially available raw materials were used unless otherwise specified. The arbutin used below is β-arbutin.

<Production of Effervescent External Skin Preparation>
Each component shown in Tables 1 to 6 was mixed so as to have the composition shown in the same table, and an external preparation kit for skin of each Example and Comparative Example was produced. In each of the examples and comparative examples described in Tables 1 to 6, the agent containing no water is in the form of powder, the agent containing water and a thickening agent is in the form of gel, and the agent containing water and no thickening agent is used. The agent was liquid.
In addition, each component of the composition shown in Table 7 below was mixed to produce a powdery composition for skin external preparation.
The skin external preparation obtained by mixing the A agent and B agent of each example described in Tables 1 to 6 in the mass described in Tables 1 to 6, respectively, is easy to apply to the skin at the time of use. It was easy. Moreover, it brought about a cosmetic effect on the skin after use. In addition, the composition for external skin preparation described in Table 7 was mixed with 9 g of the composition and 27 g of water as described in Table 7, and the same was true for the external preparation for skin. In Tables 1 to 7, the acidic substance is also simply referred to as "acid", and the carbon dioxide generating substance is simply referred to as "carbonate". In addition, glucose described in "Others" also includes use as a polyhydric alcohol.

The following [Evaluation 1] was performed for each of the above Examples and Comparative Examples.
[Evaluation 1]
Two healthy women in their twenties and thirties were randomly selected as subjects. Each subject was allowed to wash the inside of the forearm with facial cleanser and rest quietly in the measurement room for 15 minutes. Next, color difference, water loss amount, stratum corneum water content, and elasticity were measured by the following methods for a 4 cm×4 cm portion of the inside of the forearm where the external foaming skin preparation was to be applied. Next, the above foaming external preparation for skin was applied to the above measurement site and allowed to stand for 10 minutes. After that, the effervescent skin preparation for external use was removed and washed with water. 15 minutes after washing, the same measurement was performed after using the effervescent external preparation for skin. During the measurement, the subject sat quietly in the measurement room.
The skin condition measurement results after use are shown in FIGS. 1A to 13B. The values in FIGS. 1A to 13B are relative values (rates of change) when the measured value before using the foaming external preparation for skin is set to 1. The larger the color difference value, the whiter the color. , indicates that the higher the elasticity, the better.

The skin condition before and after application of the foaming external preparation for skin was measured using the following equipment. Moreover, these measurements were carried out according to standard methods described in the manual attached to the instrument.
Color difference: Measured with a color difference meter (Konica Minolta CR-400).
- Amount of water loss: Measured using a Tevameter (registered trademark) TM300 (CK Corporation).
- Stratum corneum moisture content: Measured with a skin surface stratum corneum moisture content measuring device SKICON-200EX (manufactured by IBS Co., Ltd.). This skin surface stratum corneum water content measuring device evaluates skin conductance (electrical conductivity, unit: μS) as the water content of the stratum corneum.
- Elasticity: Measured with a skin viscoelasticity measuring device Cutometer MPA580 (manufactured by Courage + Khazaka).

As shown in FIGS. 1A to 13B, the foaming topical skin preparations of each example containing the specific component showed a higher color difference and sharper corners before use than the foaming topical skin preparations of each comparative example that did not contain the specific component. The increase in layer moisture content and elasticity was large, and the decrease in moisture loss was small. Therefore, it is clear that the skin condition improving effect of the foaming external preparation for skin of the present invention containing the specific component is high.

The following [Evaluation 2] was performed for each of the above Examples and Comparative Examples.
[Evaluation 2]
Three healthy women in their twenties and thirties were randomly selected as subjects. Each subject was allowed to wash the inside of the forearm with facial cleanser and rest quietly in the measurement room for 15 minutes. Next, the foaming skin preparation for external use was applied to the measurement site on the inner side of the forearm and allowed to stand for 10 minutes. Next, the effervescent topical skin preparation was removed and washed with water. Evaluation items (16 items) during use and after use were evaluated based on a seven-grade evaluation standard. The evaluation criteria are shown in Table A below, and the results are shown in Tables B to G below. In addition, the following results are average values of evaluation points of three persons.

As shown in Tables B to G, the effervescent topical skin preparations of each example containing the specific component had a better feeling during use (easy to mix) than the effervescent topical skin preparations of each comparative example that did not contain the specific ingredient. smoothness of foam, fineness of foam, uniformity of foam, ease of application, resistance to sagging, elasticity of foam, carbonic acid feeling and its persistence), along with a feeling after use (moisturizing of the skin, elasticity, brightness, redness, smoothness, smoothness, and firmness). Therefore, the effervescent topical preparation for skin of the present invention containing specific ingredients has a high skin condition-improving effect, whitening, improving skin quality, tightening skin, conditioning skin, conditioning skin texture, tightening skin, and moisturizing skin. , keeping the skin supple, softening the skin, giving the skin firmness, smoothing the skin, and reducing the appearance of fine wrinkles caused by dryness.

The following [Evaluation 3] was performed for each of the above Examples and Comparative Examples.
[Evaluation 3] (turnover test)
A test subject (one healthy woman in her twenties to thirties selected at random) was allowed to wash the inside of her forearm with facial cleanser and rest quietly in the measurement room for 15 minutes. Next, cellophane tape (4 cm×1.8 cm) was attached to the measurement site on the inner side of the forearm, adhered with a constant pressure, and then the tape was peeled off, which was repeated twice. The mixed effervescent skin preparation for external use was applied to the measurement site in an area of 4 cm x 4 cm and allowed to stand for 10 minutes. Next, the effervescent topical skin preparation was removed and washed with water. After 5 minutes, the operation of attaching the tape to peeling off was repeated twice at the measurement site that was not attached before the application.
The first tape was discarded, and a vinyl chloride resin-based adhesive was spread thinly on the stratum corneum-collecting surface of the second tape, which was attached to a slide glass. After the application, the slide glass was sufficiently dried, immersed in ethanol for 10 minutes, and dried at room temperature (22° C. to 27° C.) for 10 minutes or longer. After that, it was immersed in xylene until the tape was peeled off. After tape removal, the slides were further immersed in xylene for 30 minutes to 1 hour. The slide glass was taken out, dried, and immersed in a staining solution (0.1 w/w% gentian violet and 0.5 w/w% brilliant green dissolved in purified water) for 15 minutes. After washing with purified water 4 to 5 times, it was dried and observed with an optical microscope at a magnification of 200. The observed images obtained were evaluated according to the evaluation criteria shown in Table H below, and evaluated on a 5-point scale for each of the three items shown in Table H below. Calculate the total value of the three evaluation points (score: 5 to 1 point) for each observation image before and after use of the foaming external preparation for skin, and calculate the total score value before use from the total score value after use. A subtracted value was obtained. All three items in Table H below indicate the degree of skin metabolism (turnover), and the higher the score, the greater the degree of turnover. 14 to 26 show the differences in the score total values obtained above before and after use. Fig. 27 shows the observed images of stratum corneum cells before and after the use of the effervescent topical skin preparations of Comparative Example 1, Example 1-19, Example 2-3, and Example 4-3 obtained by the microscopic observation. shown in

As shown in FIGS. 14 to 26, in each example containing the specific component, the total score after using the effervescent external preparation for skin increased by 2 or more from before use. On the other hand, in Comparative Examples 1 to 7, the total score value after use of the effervescent external preparation for skin decreased from that before use, or even if it increased, the difference from before use remained at 1 or less. Also from FIG. 27, it can be seen that the morphology of the stratum corneum and the arrangement order, which are indicators of turnover, are significantly improved after use of the effervescent external preparation for skin compared to before use in the example, whereas they are not obtained in the comparative example. Clearly not. Therefore, it is clear that the effervescent topical preparation for skin of each example is superior in the effect of promoting turnover as compared with each comparative example.

The specific component used in the present invention was subjected to the cell activation test of the procedures (1) to (5) below.
[Cell activation test 1]
(1) Normal human dermal fibroblasts (NHDF) were cultured in Dulbecco's Modified Eagle's Medium (10% by weight FBS-DMEM) containing 10% by weight fetal bovine serum using a 75 cm ² flask at 37°C in a 5% by volume CO ₂ incubator. was cultured by
(2) The cells cultured in (1) were suspended by trypsin treatment, and then seeded from a 75 cm ² flask to each well of a 96-well plate at a cell density of 1×10 ⁴ cells/well.
(3) The cells seeded in (2) were pre-cultured in a 10 wt% FBS-DMEM medium at 37°C in a 5% by volume CO ₂ incubator for 24 hours.
(4) After the pre-culture in (3), the medium was removed from each well, washed once with 200 μL/well of serum-free DMEM, and then 200 μL of a test substance-containing medium adjusted to a predetermined concentration was added to each well. After addition of the test substance-containing medium, the cells were cultured for 24 hours in a 5% by volume CO ₂ incubator at 37°C.
A test substance-containing medium was prepared according to Preparation Method 1 below.
(Method 1 for preparing test substance-containing medium)
Sodium bicarbonate, malic acid, a thickener listed in Table 8 below, and arbutin as a specific component, sodium bicarbonate with a final concentration of 62.5 mM, malic acid with a final concentration of 20.8 mM, a thickener and a specific The final concentrations of the components were mixed in serum-free DMEM to the values shown in Table 8 below.

(5) The cultured cells were washed once with 200 μL/well of PBS.
150 μL/well of Cell Counting Kit-8 solution diluted 30 times by volume with serum-free DMEM was added. After that, it was allowed to stand still in a 37° C., 5 vol % CO ₂ incubator to develop an appropriate color, and then the absorbance at 450 nm was measured.
Based on the obtained absorbance, the cell activation activity (%) was calculated by the following formula. The data of Reference Example 1 was used as the data control in the following formula. Cell activation activity (%) = (Data sample-Data blank) / (Data control-Data blank) x 100

FIG. 28 shows the results of cell activation activity. As shown in FIG. 28, it can be seen that the cell activating activity can be enhanced by combining the specific component with the thickening agent, the acidic component and the carbon dioxide generating component.

In addition, the specific components shown in Table 9 below were subjected to the following [cell activation test 2].
[Cell activation test 2]
The preparation method of the test substance-containing medium was changed to preparation method 2 below. In addition, serum-free DMEM to which no test substance was added was used as a data control when calculating the cell activation activity (%). Except for these points, it was the same as the above [Cell Activation Test 1]. The results are shown in Table 9 below.
(Method 2 for preparing test substance-containing medium)
Specific components shown in Table 9 below were mixed in serum-free DMEM so that the final concentrations were the values shown in Table 9 below. Further, in Table 9 below, when the item "carbonic acid" is described as "present", the final concentration of sodium hydrogen carbonate is 31.2 mM and the final concentration of malic acid is 6.25 mM. Both components were mixed in serum-free DMEM. In Table 9 below, when the item of "carbonic acid" is described as "-", only specific components were mixed in serum-free DMEM, and sodium bicarbonate and malic acid were not used.

As described above, it was confirmed that the cell activating activity was enhanced by containing the specific component in the effervescent external preparation for skin.

Claims (2)

a first agent that is an acid- and base-containing composition containing an acidic substance and a carbon dioxide-generating substance that reacts with the acidic substance to generate carbon dioxide gas;
and a second agent, which is a composition containing water, and a carbon dioxide effervescent external preparation for skin, which is a kit for mixing the first agent and the second agent at the time of use,
The second agent has a thickening agent, and
One or both of the first agent and the second agent contain at least one selected from dipotassium glycyrrhizinate, arbutin and allantoin. a first agent that is an acid- and base-containing composition containing an acidic substance and a carbon dioxide-generating substance that reacts with the acidic substance to generate carbon dioxide gas;
and a second agent that is a composition containing water,
A carbon dioxide effervescent external preparation for skin that is a kit for mixing a first agent and a second agent at the time of use, wherein one or both of the first agent and the second agent have a thickening agent, and
A carbon dioxide effervescent external preparation for skin, wherein the first agent contains at least one selected from dipotassium glycyrrhizinate, arbutin and allantoin, and the second agent contains more than 10% by mass and 80% by mass or less of a polyhydric alcohol.

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