JP7287942B2 - 高強度標識反応物組成物および配列決定のための方法 - Google Patents

高強度標識反応物組成物および配列決定のための方法 Download PDF

Info

Publication number
JP7287942B2
JP7287942B2 JP2020503775A JP2020503775A JP7287942B2 JP 7287942 B2 JP7287942 B2 JP 7287942B2 JP 2020503775 A JP2020503775 A JP 2020503775A JP 2020503775 A JP2020503775 A JP 2020503775A JP 7287942 B2 JP7287942 B2 JP 7287942B2
Authority
JP
Japan
Prior art keywords
nucleic acid
luminescent
label
attached
linker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020503775A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020530988A5 (enExample
JP2020530988A (ja
Inventor
エム． ロスバーグ、ジョナサン
ラッキー、ジェレミー
リード、ブライアン
シー、シンホア
ホワン、ハイドン
ドッド、デイビッド
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of JP2020530988A publication Critical patent/JP2020530988A/ja
Publication of JP2020530988A5 publication Critical patent/JP2020530988A5/ja
Application granted granted Critical
Publication of JP7287942B2 publication Critical patent/JP7287942B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/173—Purine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/185—Modifications characterised by incorporating bases where the precise position of the bases in the nucleic acid string is important
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/313—Branched oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/103—Nucleic acid detection characterized by the use of physical, structural and functional properties luminescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
JP2020503775A 2017-07-24 2018-07-24 高強度標識反応物組成物および配列決定のための方法 Active JP7287942B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762536426P 2017-07-24 2017-07-24
US62/536,426 2017-07-24
PCT/US2018/043526 WO2019023257A1 (en) 2017-07-24 2018-07-24 HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS

Publications (3)

Publication Number Publication Date
JP2020530988A JP2020530988A (ja) 2020-11-05
JP2020530988A5 JP2020530988A5 (enExample) 2021-09-16
JP7287942B2 true JP7287942B2 (ja) 2023-06-06

Family

ID=65018431

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020503775A Active JP7287942B2 (ja) 2017-07-24 2018-07-24 高強度標識反応物組成物および配列決定のための方法

Country Status (10)

Country Link
US (2) US11655504B2 (enExample)
EP (1) EP3658682A4 (enExample)
JP (1) JP7287942B2 (enExample)
KR (1) KR102626317B1 (enExample)
CN (1) CN111148850B (enExample)
AU (1) AU2018308098B2 (enExample)
BR (1) BR112020000697A2 (enExample)
CA (1) CA3069985A1 (enExample)
TW (1) TWI829642B (enExample)
WO (1) WO2019023257A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018308098B2 (en) 2017-07-24 2025-04-03 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
EP3820943A1 (en) 2018-07-13 2021-05-19 Quantum-si Incorporated Biconjugatable labels and methods of use
MX2021005757A (es) 2018-11-15 2021-08-11 Quantum Si Inc Metodos y composiciones para la secuenciacion de proteinas.
BR112021013787A2 (pt) 2019-01-23 2021-10-05 Quantum-Si Incorporated Composições reagentes marcadas com alta densidade e métodos para sequenciamento
JP2023527764A (ja) 2020-05-20 2023-06-30 クアンタム－エスアイ インコーポレイテッド タンパク質シーケンスのための方法及び組成物
GB2599729A (en) * 2020-10-12 2022-04-13 Sumitomo Chemical Co Method comprising light emitting marker
US11969475B2 (en) 2021-07-09 2024-04-30 Dyne Therapeutics, Inc. Muscle targeting complexes and uses thereof for treating facioscapulohumeral muscular dystrophy
US11638761B2 (en) 2021-07-09 2023-05-02 Dyne Therapeutics, Inc. Muscle targeting complexes and uses thereof for treating Facioscapulohumeral muscular dystrophy
WO2023283629A1 (en) * 2021-07-09 2023-01-12 Dyne Therapeutics, Inc. Muscle targeting complexes and formulations thereof for treating facioscapulohumeral muscular dystrophy
EP4397673A4 (en) * 2021-08-31 2025-02-19 FUJIFILM Corporation COMPOUND AND MARKED BIOMATERIAL USING SAME
KR20250004770A (ko) 2022-04-15 2025-01-08 다인 세라퓨틱스, 인크. 근긴장성 이영양증을 치료하기 위한 근육 표적화 복합체 및 제제
JP2025536336A (ja) * 2022-10-21 2025-11-05 クアンタム－エスアイ インコーポレイテッド 発光標識オリゴヌクレオチド構造ならびに関連するシステムおよび方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015073523A (ja) 2013-10-11 2015-04-20 国立大学法人 東京大学 核酸の検出方法、検出プローブ、マイクロアレイ、核酸検出キット、核酸‐検出プローブ‐捕捉プローブ複合体、核酸固定化担体、及び流体デバイス

Family Cites Families (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5565322A (en) 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
DE4435728A1 (de) 1994-01-19 1995-07-20 Boehringer Mannheim Gmbh Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5597909A (en) * 1994-08-25 1997-01-28 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use
US6248518B1 (en) 1996-10-29 2001-06-19 Board Of Regents Of University Of Nebraska Method for detecting point mutations in DNA utilizing fluorescence energy transfer
GB9716231D0 (en) * 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
US6133445A (en) 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
WO1999057321A1 (en) 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6153442A (en) 1998-05-20 2000-11-28 Dade Behring Inc. Reagents and methods for specific binding assays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6232075B1 (en) 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1681356B1 (en) 1999-05-19 2011-10-19 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423536B1 (en) 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
JP2004516810A (ja) 2000-06-07 2004-06-10 リ−コール インコーポレーティッド 電荷スイッチヌクレオチド
JP2004513619A (ja) 2000-07-07 2004-05-13 ヴィジゲン　バイオテクノロジーズ　インコーポレイテッド リアルタイム配列決定
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US7556797B2 (en) 2000-10-16 2009-07-07 Mallinckrodt Inc. Minimally invasive physiological function monitoring agents
AU2002258502A1 (en) * 2001-03-12 2002-09-24 Affymetrix, Inc. Nucleic acid labeling compounds
US7041812B2 (en) 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US9389227B2 (en) 2001-09-05 2016-07-12 Posco Solid substrate comprising array of dendrons and methods for using the same
WO2003043402A2 (en) * 2001-10-19 2003-05-30 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
DE10154291B4 (de) 2001-11-05 2005-05-19 Hain Lifescience Gmbh Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
CA2482767A1 (en) 2002-04-22 2003-10-30 Joseph F. Lawler, Jr. Reagents for monitoring nuclei acid amplification and methods of using same
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
GB0422551D0 (en) * 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
WO2007045998A2 (en) 2005-07-01 2007-04-26 Dako Denmark A/S New nucleic acid base pairs
GB0514889D0 (en) * 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
DE602006016984D1 (de) 2005-12-12 2010-10-28 Us Gov Health & Human Serv Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren
US9206476B2 (en) 2005-12-21 2015-12-08 Roche Molecular Systems, Inc Sequencing and genotyping using reversibly terminating nucleotides
CA2636599C (en) 2006-01-20 2014-07-15 Starpharma Pty Limited Modified macromolecule
WO2007106900A2 (en) * 2006-03-15 2007-09-20 The Trustees Of The University Of Pennsylvania Quantitative molecular probes
US8084734B2 (en) 2006-05-26 2011-12-27 The George Washington University Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
GB0625678D0 (en) 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
CN101434988B (zh) 2007-11-16 2013-05-01 深圳华因康基因科技有限公司 一种高通量寡核苷酸测序方法
WO2009091847A2 (en) * 2008-01-14 2009-07-23 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US7968702B2 (en) 2008-03-13 2011-06-28 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
CN102084001B (zh) 2008-03-28 2015-03-18 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
WO2009145818A1 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc Single molecule loading methods and compositions
GB2461026B (en) 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
WO2010059206A2 (en) 2008-11-19 2010-05-27 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
EP2367958B1 (en) * 2008-11-25 2017-08-23 Gen-Probe Incorporated Compositions and methods for detecting small rnas, and uses thereof
WO2010065322A1 (en) 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
KR101063981B1 (ko) 2009-01-12 2011-09-14 서강대학교산학협력단 자유 라디칼 개시제 및 이를 이용한 펩타이드 서열의 동정방법
DE102009013653B4 (de) 2009-03-18 2014-09-18 Bruker Daltonik Gmbh Protein-Sequenzierung mit MALDI-Massenspektrometrie
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
EP3211095B1 (en) 2009-04-03 2019-01-02 Sequenom, Inc. Nucleic acid preparation compositions and methods
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
ES2974909T3 (es) 2010-01-19 2024-07-02 Sirigen Ii Ltd Nuevos reactivos para la amplificación dirigida de la señal de un biomarcador
US8603741B2 (en) * 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
CA2798635A1 (en) 2010-05-06 2011-11-10 Ibis Biosciences, Inc. Integrated sample preparation systems and stabilized enzyme mixtures
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8993737B2 (en) 2010-08-25 2015-03-31 Pacific Biosciences, Inc. Phospholinked dye analogs with an amino acid linker
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
CN102329884B (zh) 2011-10-20 2013-05-08 东南大学 两核苷酸同时合成dna测序方法及其应用
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9435810B2 (en) 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
US9678080B2 (en) 2013-06-14 2017-06-13 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
WO2015021079A1 (en) 2013-08-05 2015-02-12 Pacific Biosciences Of California, Inc. Protected fluorescent reagent compounds
CN103866010B (zh) 2014-02-28 2016-04-20 郭诚 一种基因测序的方法
US9970049B2 (en) 2014-04-15 2018-05-15 The United States Of America, As Represented By The Secretary Of The Navy Use of modular nucleic acid scaffolds to create nanoscale energy harvesting and focusing arrays
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
EP3543703A1 (en) 2014-09-15 2019-09-25 Board of Regents, The University of Texas System Improved single molecule peptide sequencing
EP3258952A4 (en) 2015-02-04 2018-12-05 Pacific Biosciences Of California, Inc. Multimeric protected fluorescent reagents
EP3298389B1 (en) * 2015-05-20 2021-09-29 Quantum-Si Incorporated Method of determining the sequence of a nucleic acid using time resolved luminescence
CN105001292A (zh) 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
EP3371194B1 (en) 2015-10-16 2020-12-30 Andrew Emili Protein sequencing method
CN108350491A (zh) 2015-11-18 2018-07-31 加利福尼亚太平洋生物科学股份有限公司 将核酸加载到基材上
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
AU2017268466C1 (en) 2016-05-20 2022-04-07 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
AU2018308098B2 (en) 2017-07-24 2025-04-03 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
JP7418323B2 (ja) 2017-08-24 2024-01-19 ザ ユナイテッド ステイツ オブ アメリカ， アズ リプレゼンテッド バイ ザ セクレタリー， デパートメント オブ ヘルス アンド ヒューマン サービシーズ 遠赤および近ｉｒ範囲におけるシアニンフルオロフォアの配座の束縛
EP3820943A1 (en) 2018-07-13 2021-05-19 Quantum-si Incorporated Biconjugatable labels and methods of use
US10836798B2 (en) 2018-11-08 2020-11-17 Government Of The United States Of America, As Represented By The Secretary Of Commerce Amino acid-specific binder and selectively identifying an amino acid
BR112021013787A2 (pt) 2019-01-23 2021-10-05 Quantum-Si Incorporated Composições reagentes marcadas com alta densidade e métodos para sequenciamento

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015073523A (ja) 2013-10-11 2015-04-20 国立大学法人 東京大学 核酸の検出方法、検出プローブ、マイクロアレイ、核酸検出キット、核酸‐検出プローブ‐捕捉プローブ複合体、核酸固定化担体、及び流体デバイス

Also Published As

Publication number Publication date
WO2019023257A1 (en) 2019-01-31
KR20200029581A (ko) 2020-03-18
TW201908484A (zh) 2019-03-01
AU2018308098A1 (en) 2020-01-30
AU2018308098B2 (en) 2025-04-03
CA3069985A1 (en) 2019-01-31
EP3658682A1 (en) 2020-06-03
CN111148850A (zh) 2020-05-12
US20240068027A1 (en) 2024-02-29
US11655504B2 (en) 2023-05-23
US20190024168A1 (en) 2019-01-24
KR102626317B1 (ko) 2024-01-18
BR112020000697A2 (pt) 2020-07-14
TWI829642B (zh) 2024-01-21
JP2020530988A (ja) 2020-11-05
CN111148850B (zh) 2024-11-08
EP3658682A4 (en) 2021-06-02

Similar Documents

Publication Publication Date Title
JP7287942B2 (ja) 高強度標識反応物組成物および配列決定のための方法
JP7618563B2 (ja) 高強度標識されたシーケンシング用の反応組成物および方法
EP2768972B2 (en) Methods and compositions for nucleic acid sequencing
JP6085564B2 (ja) 標的核酸の検出法
US10851407B2 (en) Left-handed gamma-peptide nucleic acids, methods of synthesis and uses therefor
JP2019523756A (ja) 標識ヌクレオチド組成物および核酸の配列を決定するための方法
CN101285094A (zh) 引物、成套引物、使用其的核酸扩增方法和变异检测方法
KR101880502B1 (ko) 메로시아닌계 화합물, 이를 포함하는 생체분자 표지용 염료, 키트 및 조영제 조성물
US20230272470A1 (en) Ultrabright dna nanostructures for biosensing
HK40073095A (en) Compositions for nucleic acid sequencing
HK40014484A (en) Compositions for nucleic acid sequencing
HK40014484B (en) Compositions for nucleic acid sequencing
HK1201079B (en) Methods and compositions for nucleic acid sequencing
PT763133E (pt) Metodo para a deteccao de acidos nucleicos alvo

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210721

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210721

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210804

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20220520

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220705

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220921

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230105

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230425

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230525

R150 Certificate of patent or registration of utility model

Ref document number: 7287942

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150