BR112020000697A2 - composições reagentes marcadas de alta intensidade e métodos para sequenciamento - Google Patents

composições reagentes marcadas de alta intensidade e métodos para sequenciamento Download PDF

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Publication number
BR112020000697A2
BR112020000697A2 BR112020000697-1A BR112020000697A BR112020000697A2 BR 112020000697 A2 BR112020000697 A2 BR 112020000697A2 BR 112020000697 A BR112020000697 A BR 112020000697A BR 112020000697 A2 BR112020000697 A2 BR 112020000697A2
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BR
Brazil
Prior art keywords
nucleic acid
luminescent
labeled nucleotide
marker
labeled
Prior art date
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BR112020000697-1A
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English (en)
Portuguese (pt)
Inventor
Jonathan M. Rothberg
Jeremy Lackey
Brian Reed
Xinghua Shi
Haidong Huang
David Dodd
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Quantum-Si Incorporated
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Application filed by Quantum-Si Incorporated filed Critical Quantum-Si Incorporated
Publication of BR112020000697A2 publication Critical patent/BR112020000697A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/173—Purine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/185—Modifications characterised by incorporating bases where the precise position of the bases in the nucleic acid string is important
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/313—Branched oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/103—Nucleic acid detection characterized by the use of physical, structural and functional properties luminescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
BR112020000697-1A 2017-07-24 2018-07-24 composições reagentes marcadas de alta intensidade e métodos para sequenciamento BR112020000697A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762536426P 2017-07-24 2017-07-24
US62/536,426 2017-07-24
PCT/US2018/043526 WO2019023257A1 (en) 2017-07-24 2018-07-24 HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS

Publications (1)

Publication Number Publication Date
BR112020000697A2 true BR112020000697A2 (pt) 2020-07-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112020000697-1A BR112020000697A2 (pt) 2017-07-24 2018-07-24 composições reagentes marcadas de alta intensidade e métodos para sequenciamento

Country Status (10)

Country Link
US (2) US11655504B2 (enExample)
EP (1) EP3658682A4 (enExample)
JP (1) JP7287942B2 (enExample)
KR (1) KR102626317B1 (enExample)
CN (1) CN111148850B (enExample)
AU (1) AU2018308098B2 (enExample)
BR (1) BR112020000697A2 (enExample)
CA (1) CA3069985A1 (enExample)
TW (1) TWI829642B (enExample)
WO (1) WO2019023257A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11655504B2 (en) 2017-07-24 2023-05-23 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
AU2019300169A1 (en) 2018-07-13 2021-01-28 Quantum-Si Incorporated Biconjugatable labels and methods of use
AU2019380606A1 (en) 2018-11-15 2021-05-27 Quantum-Si Incorporated Methods and compositions for protein sequencing
CA3127102A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
JP2023527764A (ja) 2020-05-20 2023-06-30 クアンタム－エスアイ インコーポレイテッド タンパク質シーケンスのための方法及び組成物
GB2599729A (en) * 2020-10-12 2022-04-13 Sumitomo Chemical Co Method comprising light emitting marker
US11638761B2 (en) 2021-07-09 2023-05-02 Dyne Therapeutics, Inc. Muscle targeting complexes and uses thereof for treating Facioscapulohumeral muscular dystrophy
US11969475B2 (en) 2021-07-09 2024-04-30 Dyne Therapeutics, Inc. Muscle targeting complexes and uses thereof for treating facioscapulohumeral muscular dystrophy
WO2023283629A1 (en) * 2021-07-09 2023-01-12 Dyne Therapeutics, Inc. Muscle targeting complexes and formulations thereof for treating facioscapulohumeral muscular dystrophy
EP4397673A4 (en) * 2021-08-31 2025-02-19 FUJIFILM Corporation COMPOUND AND MARKED BIOMATERIAL USING SAME
IL316143A (en) 2022-04-15 2024-12-01 Dyne Therapeutics Inc Muscle targeting complexes and formulations for the treatment of myotonic dystrophy
AU2023364709A1 (en) * 2022-10-21 2025-05-08 Quantum-Si Incorporated Luminescently labeled oligonucleotide structures and associated systems and methods

Family Cites Families (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE69231853T2 (de) * 1991-11-07 2001-09-13 Nanotronics, Inc. Hybridisierung von mit chromophoren und fluorophoren konjugierten polynukleotiden zur erzeugung eines donor-zu-donor energietransfersystems
DE4435728A1 (de) 1994-01-19 1995-07-20 Boehringer Mannheim Gmbh Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5597909A (en) * 1994-08-25 1997-01-28 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use
US7468243B2 (en) * 2001-03-12 2008-12-23 Affymetrix, Inc. 2-aminopyrimidin-4-one nucleic acid labeling compounds
US6248518B1 (en) 1996-10-29 2001-06-19 Board Of Regents Of University Of Nebraska Method for detecting point mutations in DNA utilizing fluorescence energy transfer
GB9716231D0 (en) * 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
US6133445A (en) 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
CA2330673C (en) 1998-05-01 2009-05-26 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6153442A (en) 1998-05-20 2000-11-28 Dade Behring Inc. Reagents and methods for specific binding assays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AU772281B2 (en) 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
EP1681357A3 (en) 1999-05-19 2006-07-26 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423536B1 (en) 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
AU2001268291A1 (en) 2000-06-07 2001-12-17 Li-Cor, Inc. Charge-switch nucleotides
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
CN100462433C (zh) 2000-07-07 2009-02-18 维西根生物技术公司 实时序列测定
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US7556797B2 (en) 2000-10-16 2009-07-07 Mallinckrodt Inc. Minimally invasive physiological function monitoring agents
AU2002324827B2 (en) 2001-08-29 2007-11-29 Global Life Sciences Solutions Usa Llc Labeled nucleoside polyphosphates
US9389227B2 (en) 2001-09-05 2016-07-12 Posco Solid substrate comprising array of dendrons and methods for using the same
AU2002366046A1 (en) * 2001-10-19 2003-06-10 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
DE10154291B4 (de) 2001-11-05 2005-05-19 Hain Lifescience Gmbh Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP1497303A2 (en) 2002-04-22 2005-01-19 Joseph F. Lawler Jr. Reagents for monitoring nuclei acid amplification and methods of using same
US20070059690A1 (en) * 2002-05-31 2007-03-15 Amirul Islam "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
EP1725572B1 (de) 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
GB0422551D0 (en) * 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
US8741566B2 (en) 2005-07-01 2014-06-03 Dako Denmark A/S Immunohistochemistry and in situ hybridization detection method
GB0514889D0 (en) * 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
WO2007070572A2 (en) 2005-12-12 2007-06-21 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Probe for nucleic acid sequencing and methods of use
EP1974057B1 (en) 2005-12-21 2012-06-13 Roche Diagnostics GmbH Sequencing and genotyping using reversibly 2'-modified nucleotides
CN104524596B (zh) 2006-01-20 2018-10-09 星药股份有限公司 经修饰的大分子
WO2007106900A2 (en) * 2006-03-15 2007-09-20 The Trustees Of The University Of Pennsylvania Quantitative molecular probes
US8084734B2 (en) 2006-05-26 2011-12-27 The George Washington University Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
GB0625678D0 (en) 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
CN101434988B (zh) 2007-11-16 2013-05-01 深圳华因康基因科技有限公司 一种高通量寡核苷酸测序方法
WO2009091847A2 (en) * 2008-01-14 2009-07-23 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009114182A1 (en) 2008-03-13 2009-09-17 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
CA2719747C (en) 2008-03-28 2018-02-20 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
CA2720247C (en) 2008-03-31 2020-07-14 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
GB2461026B (en) 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
US8252910B2 (en) 2008-11-19 2012-08-28 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
CN102292457B (zh) * 2008-11-25 2014-04-09 简·探针公司 检测小rna的组合物和方法及其用途
WO2010065322A1 (en) 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
KR101063981B1 (ko) 2009-01-12 2011-09-14 서강대학교산학협력단 자유 라디칼 개시제 및 이를 이용한 펩타이드 서열의 동정방법
DE102009013653B4 (de) 2009-03-18 2014-09-18 Bruker Daltonik Gmbh Protein-Sequenzierung mit MALDI-Massenspektrometrie
WO2010111686A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Labeled enzyme compositions, methods & systems
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US8771948B2 (en) 2009-04-03 2014-07-08 Sequenom, Inc. Nucleic acid preparation compositions and methods
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
EP3981819B1 (en) 2010-01-19 2024-02-28 Sirigen II Limited Novel reagents for directed biomarker signal amplification
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
SG10201503540QA (en) 2010-05-06 2015-06-29 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8993737B2 (en) 2010-08-25 2015-03-31 Pacific Biosciences, Inc. Phospholinked dye analogs with an amino acid linker
WO2012129242A2 (en) 2011-03-23 2012-09-27 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
CN102329884B (zh) 2011-10-20 2013-05-08 东南大学 两核苷酸同时合成dna测序方法及其应用
US9062091B2 (en) 2012-02-15 2015-06-23 Pacific Biosciences Of California, Inc. Polymerase enzyme substrates with protein shield
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9435810B2 (en) 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
EP3008094B1 (en) 2013-06-14 2018-12-26 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
WO2015021079A1 (en) 2013-08-05 2015-02-12 Pacific Biosciences Of California, Inc. Protected fluorescent reagent compounds
JP2015073523A (ja) * 2013-10-11 2015-04-20 国立大学法人 東京大学 核酸の検出方法、検出プローブ、マイクロアレイ、核酸検出キット、核酸‐検出プローブ‐捕捉プローブ複合体、核酸固定化担体、及び流体デバイス
CN103866010B (zh) 2014-02-28 2016-04-20 郭诚 一种基因测序的方法
US9970049B2 (en) 2014-04-15 2018-05-15 The United States Of America, As Represented By The Secretary Of The Navy Use of modular nucleic acid scaffolds to create nanoscale energy harvesting and focusing arrays
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
US10545153B2 (en) 2014-09-15 2020-01-28 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
US10150872B2 (en) 2015-02-04 2018-12-11 Pacific Biosciences Of California, Inc. Multimeric protected fluorescent reagents
CN108027325A (zh) * 2015-05-20 2018-05-11 宽腾矽公司 采用时间分辨发光确定核酸序列的方法
CN105001292A (zh) 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
US11268963B2 (en) 2015-10-16 2022-03-08 The Governing Council Of The University Of Toronto Protein sequencing methods and reagents
WO2017087702A1 (en) 2015-11-18 2017-05-26 Pacific Biosciences Of California, Inc. Loading nucleic acids onto substrates
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
EP4428528A3 (en) * 2016-05-20 2025-04-23 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
US11655504B2 (en) 2017-07-24 2023-05-23 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2019040825A1 (en) 2017-08-24 2019-02-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services CONFORMATIONAL RESTRICTION OF CYANINE FLUOROPHORES IN A FAR RED RANGE AND CLOSE INFRARED
AU2019300169A1 (en) 2018-07-13 2021-01-28 Quantum-Si Incorporated Biconjugatable labels and methods of use
US10836798B2 (en) 2018-11-08 2020-11-17 Government Of The United States Of America, As Represented By The Secretary Of Commerce Amino acid-specific binder and selectively identifying an amino acid
CA3127102A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing

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