JP7284697B2 - 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法 - Google Patents

液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法 Download PDF

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Publication number
JP7284697B2
JP7284697B2 JP2019224176A JP2019224176A JP7284697B2 JP 7284697 B2 JP7284697 B2 JP 7284697B2 JP 2019224176 A JP2019224176 A JP 2019224176A JP 2019224176 A JP2019224176 A JP 2019224176A JP 7284697 B2 JP7284697 B2 JP 7284697B2
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electrospray ionization
ionization source
protein
exemplary embodiments
sheath gas
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JP2020101538A (ja
JP2020101538A5 (https=
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シュンハイ ワン
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H01J49/167—Capillaries and nozzles specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Plasma & Fusion (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
JP2019224176A 2018-12-12 2019-12-12 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法 Active JP7284697B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2023082905A JP7554312B2 (ja) 2018-12-12 2023-05-19 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法
JP2024153804A JP2024166268A (ja) 2018-12-12 2024-09-06 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法

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US201862778521P 2018-12-12 2018-12-12
US62/778,521 2018-12-12

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JP2023082905A Division JP7554312B2 (ja) 2018-12-12 2023-05-19 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法

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JP2020101538A JP2020101538A (ja) 2020-07-02
JP2020101538A5 JP2020101538A5 (https=) 2022-12-16
JP7284697B2 true JP7284697B2 (ja) 2023-05-31

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JP2019224176A Active JP7284697B2 (ja) 2018-12-12 2019-12-12 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法
JP2023082905A Active JP7554312B2 (ja) 2018-12-12 2023-05-19 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法
JP2024153804A Withdrawn JP2024166268A (ja) 2018-12-12 2024-09-06 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法

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JP2024153804A Withdrawn JP2024166268A (ja) 2018-12-12 2024-09-06 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法

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US (3) US11249089B2 (https=)
EP (1) EP3667314A1 (https=)
JP (3) JP7284697B2 (https=)
CA (1) CA3064599A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11249089B2 (en) * 2018-12-12 2022-02-15 Regeneron Pharmaceuticals, Inc. System and method of analysis of a protein using liquid chromatography-mass spectrometry
BR112022015097A2 (pt) * 2020-01-30 2022-09-20 Regeneron Pharma Plataforma para espectrometria de massa de cromatografia líquida nativa
CN112305132B (zh) * 2020-11-30 2023-03-24 浙江理工大学 一种氯化石蜡氯含量的测定方法及系统
JP2024512509A (ja) * 2021-03-31 2024-03-19 レメゲン シーオー．，エルティーディー． Ｌｃ－ｍｓ／ｍｓ法によるａｄｃ中のｔｃｅｐ含有量の検出方法

Citations (2)

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US20070102634A1 (en) 2005-11-10 2007-05-10 Frey Brian L Electrospray ionization ion source with tunable charge reduction
US20160329198A1 (en) 2015-05-05 2016-11-10 Ohio State Innovation Foundation Method and apparatus for contained-electrospray for use in mass spectrometry and droplet reactions

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WO2000078447A1 (en) * 1999-06-18 2000-12-28 Tsi Incorporated Aerosol charge adjusting apparatus employing a corona discharge
FR2896585B1 (fr) * 2006-01-20 2008-12-05 Commissariat Energie Atomique Introduction d'additifs pour une interface d'ionisation a pression atmospherique en entree d'un spectrometre
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
US8242441B2 (en) * 2009-12-18 2012-08-14 Thermo Finnigan Llc Apparatus and methods for pneumatically-assisted electrospray emitter array
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
US9074236B2 (en) * 2012-05-01 2015-07-07 Oxoid Limited Apparatus and methods for microbial identification by mass spectrometry
JOP20200236A1 (ar) 2012-09-21 2017-06-16 Regeneron Pharma الأجسام المضادة لمضاد cd3 وجزيئات ربط الأنتيجين ثنائية التحديد التي تربط cd3 وcd20 واستخداماتها
US8759753B1 (en) * 2013-03-14 2014-06-24 Thermo Finnigan Llc Methods and apparatus for improved immunosuppressant drug monitoring
GB2527803B (en) * 2014-07-02 2018-02-07 Microsaic Systems Plc A method and system for monitoring biomolecule separations by mass spectrometry
AR101262A1 (es) 2014-07-26 2016-12-07 Regeneron Pharma Plataforma de purificación para anticuerpos biespecíficos
JOP20160154B1 (ar) 2015-07-31 2021-08-17 Regeneron Pharma أجسام ضادة مضاد لل psma، وجزيئات رابطة لمستضد ثنائي النوعية الذي يربط psma و cd3، واستخداماتها
LT3394103T (lt) 2015-12-22 2023-09-11 Regeneron Pharmaceuticals, Inc. Antikūnų prieš pd-1 ir bispecifinių antikūnų prieš cd20/cd3 derinys, skirtas vėžiui gydyti
AU2016378573A1 (en) 2015-12-22 2018-07-19 Regeneron Pharmaceuticals, Inc. Bispecific anti-CD20/anti-CD3 antibodies to treat acute lymphoblastic leukemia
JP6734061B2 (ja) * 2016-01-29 2020-08-05 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． プラズマ分光分析装置
MY199468A (en) 2016-09-23 2023-10-31 Regeneron Pharma Anti-steap2 antibodies, antibody-drug conjugates, and bispecific antigen-binding molecules that bind steap2 and cd3, and uses thereof
JP7066690B2 (ja) 2016-09-23 2022-05-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 抗ｍｕｃ１６（ムチン１６）抗体
TWI782930B (zh) 2016-11-16 2022-11-11 美商再生元醫藥公司 抗ｍｅｔ抗體，結合ｍｅｔ之雙特異性抗原結合分子及其使用方法
JP6988158B2 (ja) * 2017-05-11 2022-01-05 株式会社島津製作所 液体クロマトグラフ質量分析方法及び液体クロマトグラフ質量分析装置
JP6775826B2 (ja) * 2017-06-05 2020-10-28 有限会社ラブディポット 高気密保存容器用キャップ
US11249089B2 (en) * 2018-12-12 2022-02-15 Regeneron Pharmaceuticals, Inc. System and method of analysis of a protein using liquid chromatography-mass spectrometry

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Publication number Priority date Publication date Assignee Title
US20070102634A1 (en) 2005-11-10 2007-05-10 Frey Brian L Electrospray ionization ion source with tunable charge reduction
US20160329198A1 (en) 2015-05-05 2016-11-10 Ohio State Innovation Foundation Method and apparatus for contained-electrospray for use in mass spectrometry and droplet reactions

Non-Patent Citations (2)

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KHARLAMOVA A, et al.，Negative Electrospray Droplet Exposure to Gaseous Bases for the Manipulation of Protein Charge State Distributions，Analytical Chemistry，2010年12月09日，Vol. 83, No. 1，pp.431-437，10.1021/ac1027319
LI Z , et al.，Chemical-Vapor-Assisted Electrospray Ionization for Increasing Analyte Signals in Electrospray Ionization Mass Spectrometry，Analytical Chemistry，Vol.86，2013年12月23日，pp.331-335，dx.doi.org/10.1021/ac4036263

Also Published As

Publication number Publication date
US12174199B2 (en) 2024-12-24
US20240219397A1 (en) 2024-07-04
US11835528B2 (en) 2023-12-05
JP2020101538A (ja) 2020-07-02
JP7554312B2 (ja) 2024-09-19
JP2024166268A (ja) 2024-11-28
US20220113317A1 (en) 2022-04-14
EP3667314A1 (en) 2020-06-17
US11249089B2 (en) 2022-02-15
US20200191795A1 (en) 2020-06-18
JP2023116475A (ja) 2023-08-22
CA3064599A1 (en) 2020-06-12

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