CA3064599A1 - System and method of analysis of a protein using liquid chromatography-mass spectrometry - Google Patents

System and method of analysis of a protein using liquid chromatography-mass spectrometry Download PDF

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Publication number
CA3064599A1
CA3064599A1 CA3064599A CA3064599A CA3064599A1 CA 3064599 A1 CA3064599 A1 CA 3064599A1 CA 3064599 A CA3064599 A CA 3064599A CA 3064599 A CA3064599 A CA 3064599A CA 3064599 A1 CA3064599 A1 CA 3064599A1
Authority
CA
Canada
Prior art keywords
electrospray ionization
protein
ionization source
electrospray
container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3064599A
Other languages
English (en)
French (fr)
Inventor
Shunhai Wang
Original Assignee
Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of CA3064599A1 publication Critical patent/CA3064599A1/en
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H01J49/167—Capillaries and nozzles specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Plasma & Fusion (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
CA3064599A 2018-12-12 2019-12-11 System and method of analysis of a protein using liquid chromatography-mass spectrometry Pending CA3064599A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862778521P 2018-12-12 2018-12-12
US62/778,521 2018-12-12

Publications (1)

Publication Number Publication Date
CA3064599A1 true CA3064599A1 (en) 2020-06-12

Family

ID=68916238

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3064599A Pending CA3064599A1 (en) 2018-12-12 2019-12-11 System and method of analysis of a protein using liquid chromatography-mass spectrometry

Country Status (4)

Country Link
US (3) US11249089B2 (https=)
EP (1) EP3667314A1 (https=)
JP (3) JP7284697B2 (https=)
CA (1) CA3064599A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11249089B2 (en) * 2018-12-12 2022-02-15 Regeneron Pharmaceuticals, Inc. System and method of analysis of a protein using liquid chromatography-mass spectrometry
BR112022015097A2 (pt) * 2020-01-30 2022-09-20 Regeneron Pharma Plataforma para espectrometria de massa de cromatografia líquida nativa
CN112305132B (zh) * 2020-11-30 2023-03-24 浙江理工大学 一种氯化石蜡氯含量的测定方法及系统
JP2024512509A (ja) * 2021-03-31 2024-03-19 レメゲン シーオー．，エルティーディー． Ｌｃ－ｍｓ／ｍｓ法によるａｄｃ中のｔｃｅｐ含有量の検出方法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000078447A1 (en) * 1999-06-18 2000-12-28 Tsi Incorporated Aerosol charge adjusting apparatus employing a corona discharge
US7518108B2 (en) * 2005-11-10 2009-04-14 Wisconsin Alumni Research Foundation Electrospray ionization ion source with tunable charge reduction
FR2896585B1 (fr) * 2006-01-20 2008-12-05 Commissariat Energie Atomique Introduction d'additifs pour une interface d'ionisation a pression atmospherique en entree d'un spectrometre
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
US8242441B2 (en) * 2009-12-18 2012-08-14 Thermo Finnigan Llc Apparatus and methods for pneumatically-assisted electrospray emitter array
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
US9074236B2 (en) * 2012-05-01 2015-07-07 Oxoid Limited Apparatus and methods for microbial identification by mass spectrometry
JOP20200236A1 (ar) 2012-09-21 2017-06-16 Regeneron Pharma الأجسام المضادة لمضاد cd3 وجزيئات ربط الأنتيجين ثنائية التحديد التي تربط cd3 وcd20 واستخداماتها
US8759753B1 (en) * 2013-03-14 2014-06-24 Thermo Finnigan Llc Methods and apparatus for improved immunosuppressant drug monitoring
GB2527803B (en) * 2014-07-02 2018-02-07 Microsaic Systems Plc A method and system for monitoring biomolecule separations by mass spectrometry
AR101262A1 (es) 2014-07-26 2016-12-07 Regeneron Pharma Plataforma de purificación para anticuerpos biespecíficos
US9852896B2 (en) * 2015-05-05 2017-12-26 Ohio State Innovation Foundation Method and apparatus for contained-electrospray for use in mass spectrometry and droplet reactions
JOP20160154B1 (ar) 2015-07-31 2021-08-17 Regeneron Pharma أجسام ضادة مضاد لل psma، وجزيئات رابطة لمستضد ثنائي النوعية الذي يربط psma و cd3، واستخداماتها
LT3394103T (lt) 2015-12-22 2023-09-11 Regeneron Pharmaceuticals, Inc. Antikūnų prieš pd-1 ir bispecifinių antikūnų prieš cd20/cd3 derinys, skirtas vėžiui gydyti
AU2016378573A1 (en) 2015-12-22 2018-07-19 Regeneron Pharmaceuticals, Inc. Bispecific anti-CD20/anti-CD3 antibodies to treat acute lymphoblastic leukemia
JP6734061B2 (ja) * 2016-01-29 2020-08-05 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． プラズマ分光分析装置
MY199468A (en) 2016-09-23 2023-10-31 Regeneron Pharma Anti-steap2 antibodies, antibody-drug conjugates, and bispecific antigen-binding molecules that bind steap2 and cd3, and uses thereof
JP7066690B2 (ja) 2016-09-23 2022-05-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 抗ｍｕｃ１６（ムチン１６）抗体
TWI782930B (zh) 2016-11-16 2022-11-11 美商再生元醫藥公司 抗ｍｅｔ抗體，結合ｍｅｔ之雙特異性抗原結合分子及其使用方法
JP6988158B2 (ja) * 2017-05-11 2022-01-05 株式会社島津製作所 液体クロマトグラフ質量分析方法及び液体クロマトグラフ質量分析装置
JP6775826B2 (ja) * 2017-06-05 2020-10-28 有限会社ラブディポット 高気密保存容器用キャップ
US11249089B2 (en) * 2018-12-12 2022-02-15 Regeneron Pharmaceuticals, Inc. System and method of analysis of a protein using liquid chromatography-mass spectrometry

Also Published As

Publication number Publication date
US12174199B2 (en) 2024-12-24
US20240219397A1 (en) 2024-07-04
US11835528B2 (en) 2023-12-05
JP7284697B2 (ja) 2023-05-31
JP2020101538A (ja) 2020-07-02
JP7554312B2 (ja) 2024-09-19
JP2024166268A (ja) 2024-11-28
US20220113317A1 (en) 2022-04-14
EP3667314A1 (en) 2020-06-17
US11249089B2 (en) 2022-02-15
US20200191795A1 (en) 2020-06-18
JP2023116475A (ja) 2023-08-22

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