JP7245191B2 - マイクロ流体デバイス - Google Patents
マイクロ流体デバイス Download PDFInfo
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Description
dPCR反応ウェル/チャンバーの数だけでなく、それぞれの体積もまた、マイクロ流体デバイス上の所定の領域に関して、最大でなければならないことが見出されてきている。しかし、こうしたマイクロ流体デバイスの製造プロセスは、通常、射出成形により、射出成形しようとするマイクロ流体デバイスにおいて、ウェルのありうる最大数ならびにそれぞれの最大体積に関しては、特定の制約を含む。
2 支持体
3 フローチャネル
31 第一のフローチャネル表面
31’ 第一のフローチャネル表面
32 伸長した六角形のマイクロウェル
32’ 正六角形のマイクロウェル
32” さらにまたは非常に伸長した六角形のマイクロウェル
321 マイクロウェル頂点
322 マイクロウェルエッジ
33 マイクロウェルの間のリム
4 カバー
41 第二のフローチャネル表面
41’ 第二のフローチャネル表面
42 入口開口部
43 出口開口部
5 dPCR反応混合物
51 dPCR反応混合物の前線/正面
6 気泡/空気泡
7 分離/密封流体
Claims (20)
- 反応混合物(5)を熱サイクリングするためのマイクロ流体デバイス(1)であって:
入口開口部(42);
出口開口部(43);
該入口開口部(42)および該出口開口部(43)を連結し、そして該入口開口部(42)からフローチャネル(3)を通り該出口開口部(43)へ向かう流動方向を定義するフローチャネル(3)であって、該フローチャネル(3)は、第一のフローチャネル表面(31)および該第一のフローチャネル表面(31)とは反対側の第二のフローチャネル表面(41)を含む、ならびに
該入口開口部(42)および該出口開口部(43)と流体連絡するため、該第一のフローチャネル表面(31)に提供されるウェル(32)のアレイ
を含み、
該フローチャネル(3)の高さおよび各ウェル(32)の長さの間のアスペクト比が、0.3~0.7の間の範囲であり、
該第一のフローチャネル表面(31)が第一の親水性を提供し、そして該第二のフローチャネル表面(41)の少なくとも部分が第二の親水性を提供し、そして
該第一の親水性が該第二の親水性よりも大きい
前記マイクロ流体デバイス(1)。 - 前記第一の親水性および/または第二の親水性が、材料特性によって、表面処理によって、または親水性コーティングによって提供される、請求項1のマイクロ流体デバイス(1)。
- 前記表面処理がプラズマ親水性化処理であり、そして前記親水性コーティングがSiO2コーティングである、請求項2記載のマイクロ流体デバイス(1)。
- 前記ウェル(32)のアレイの少なくとも部分が、前記第一のフローチャネル表面(31)において、六角形型のウェル形状を示し、または、すべてのウェル(32)が、第一のフローチャネル表面(31)において、六角形型のウェル形状を示す、請求項1~3のいずれか一項のマイクロ流体デバイス(1)。
- 各六角形ウェル(32)の頂点(321)が、流動方向中で、入口開口部(42)の側を向いて配向されている、請求項4のマイクロ流体デバイス(1)。
- 互いに反対側に配置された各六角形ウェル(32)の2つの頂点が、流動方向に平行に配向されている、請求項5のマイクロ流体デバイス(1)。
- 各六角形ウェル(32)が、流動方向に伸長した、伸長した六角形の形状を含む、請求項4~6のいずれか一項のマイクロ流体デバイス(1)。
- 各ウェル(32)が、50μm~300μmの範囲の流動方向のウェルの長さ、および/または25μm~150μmの範囲の、ウェルの長さに垂直なウェルの幅、および/または25μm~200μmの範囲のウェルの深さを含む、請求項1~7のいずれか一項のマイクロ流体デバイス(1)。
- 入口開口部(42)の側を向いた第一のフローチャネル表面(31)における各ウェル(32)のエッジ(322)が丸みを帯びたエッジである、請求項1~8のいずれか一項のマイクロ流体デバイス(1)。
- 前記丸みを帯びたウェルエッジ(322)が<10μmの半径で丸みを帯びている、請求項9のマイクロ流体デバイス(1)。
- 隣接するウェル(32)の流体分離のために、隣接するウェル(32)間にリム(33)が提供され、各リム(33)が>10μmの幅を含む、請求項1~10のいずれか一項のマイクロ流体デバイス(1)。
- 前記フローチャネル(3)の高さおよび各ウェル(32)の長さの間のアスペクト比が0.5である、請求項1~11のいずれか一項のマイクロ流体デバイス(1)。
- 前記フローチャネル(3)の高さが25μm~200μmの範囲である、請求項1~12のいずれか一項のマイクロ流体デバイス(1)。
- マイクロ流体デバイス(1)が、互いに付着可能である2つの部分(2、4)からなる、請求項1~13のいずれか一項のマイクロ流体デバイス(1)。
- マイクロ流体デバイス(1)が、その長軸方向に沿って、2つの部分(2、4)に分けられる、請求項14のマイクロ流体デバイス(1)。
- ウェル(32)のアレイ、ならびに入口開口部(42)および出口開口部(43)を含むフローチャネル(3)が、第一のフローチャネル表面(31)を提供するマイクロ流体デバイス(1)の一方の部分(2)中に提供され、そしてマイクロ流体デバイス(1)の他方の部分(4)が、第二のフローチャネル表面(41)を提供するカバー部分を構成する、請求項14または15記載のマイクロ流体デバイス(1)。
- 前記第二のフローチャネル表面(41)を提供するカバー部分は、カバープレートまたはカバーホイルの形で提供される、請求項16記載のマイクロ流体デバイス(1)。
- 前記フローチャネル(3)によって、ウェル(32)の各々に反応混合物(5)の形で提供される試料のデジタルPCRまたは生化学アッセイのために、マイクロ流体デバイス(1)を用いる、請求項1~17のいずれか一項のマイクロ流体デバイス(1)。
- マイクロ流体デバイス(1)が消耗品である、請求項1~18いずれか一項のマイクロ流体デバイス(1)。
- マイクロ流体デバイス(1)が、透明材料、環状オレフィンコポリマーCOC、または環状オレフィンポリマーCOPからなる、請求項19のマイクロ流体デバイス(1)。
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EP19166293 | 2019-03-29 |
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EP (1) | EP3714977A1 (ja) |
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CN (1) | CN111748444A (ja) |
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CN111044739A (zh) * | 2019-12-02 | 2020-04-21 | 江苏圣极基因科技有限公司 | 用于微流体芯片的样品试剂的进样方法及进样系统 |
DE102019220017A1 (de) * | 2019-12-18 | 2021-06-24 | Robert Bosch Gesellschaft mit beschränkter Haftung | Aufnahmeeinheit zum Aufnehmen eines Fluids, Verfahren und Vorrichtung zum Herstellen einer Aufnahmeeinheit, Verfahren und Vorrichtung zum Betreiben einer Aufnahmeeinheit und Aufnahmeeinrichtung |
WO2022140599A1 (en) * | 2020-12-23 | 2022-06-30 | Cornell University | Multi-volume microchamber-based microfluidic platform and use thereof |
EP4325206A1 (en) | 2022-08-18 | 2024-02-21 | F. Hoffmann-La Roche AG | Method for compensating defective partitions of a microfluidic chip |
EP4325444A1 (en) | 2022-08-18 | 2024-02-21 | Roche Diagnostics GmbH | Digital pcr leakage detection and correction methods and systems |
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