JP7197717B2 - マイクロフルイディクスチップシステム及び液滴の調製方法 - Google Patents
マイクロフルイディクスチップシステム及び液滴の調製方法 Download PDFInfo
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Description
また、前記マイクロビーズ溶液70は、ポリスチレンマイクロビーズ溶液、樹脂マイクロビーズ溶液、又は磁気ビーズ溶液等のいずれであってもよい。
10、101 調製台
11 ベース
111 第1表面
112 第2表面
113 第1側面
114 第2側面
115 第1部
116 第2部
12 液滴生成装置用スロット
13 収集瓶用スロット
14 動力発生装置用スロット
141 第1収容部
142 第2収容部
15 第1位置決め溝
18 第2位置決め溝
16 固定台
17 ダストカバー
20 液滴生成装置
21、110、120 チップ本体
211 第3表面
212 第4表面
213 油相入口
214 細胞液入口
215 マイクロビーズ溶液入口
216 液滴出口
217 油相導入通路
218 細胞液導入通路
219 マイクロビーズ溶液導入通路
210 合流通路
22 第1液溜めプール
23 第2液溜めプール
24 第3液溜めプール
25 第1振動装置
26 第2振動装置
30 収集瓶
31 カバー
40 接続装置
41 第1接続管
42 第2接続管
50 動力発生装置
51 本体部
511 収容空間
52 操作部
53 気体出入口端
54 第1位置決め部材
55 第2位置決め部材
60 細胞液
70 マイクロビーズ溶液
80 油相
200 液滴
43 第3接続管
42 第4接続管
45 第5接続管
27 凸部
Claims (15)
- 液滴を生成するマイクロフルイディクスチップシステムであって、
液滴を生成する液滴生成装置と、
本体部および前記本体部に露出する第1位置決め部材を備え、液滴を生成する動力を前記液滴生成装置に供給する動力発生装置と、
前記液滴生成装置から流出した液滴を収集する収集瓶と、
前記液滴生成装置、前記動力発生装置および前記収集瓶とを連通するための接続装置と、
ベースと、前記ベースに開設された液滴生成装置用スロット、動力発生装置用スロットおよび第1位置決め溝と、を備える調製台であって、液滴生成装置および動力発生装置が、それぞれ液滴生成装置用スロットおよび動力発生装置用スロットに収容されており、前記第1位置決め溝が、前記第1位置決め部材を収容して所定位置に保持しており、前記液滴生成装置、前記動力発生装置および前記収集瓶を固定する調製台とを、含むことを特徴するマイクロフルイディクスチップシステム。 - 前記液滴生成装置は、連続相入口及び分散相入口が形成されたチップ本体を含み、連続相及び分散相は前記連続相入口及び分散相入口から前記チップ本体に入ることを特徴する請求項1に記載のマイクロフルイディクスチップシステム。
- 前記マイクロフルイディクスチップシステムは、連続相液溜めプールおよび分散相液溜めプールをさらに含み、前記連続相液溜めプールおよび分散相液溜めプールはそれぞれ前記チップ本体上に固定されたり、または前記チップ本体外に形成されることを特徴する請求項2に記載のマイクロフルイディクスチップシステム。
- 前記ベースは、第1部と、前記第1部に接続された第2部と、を含み、前記第1部は、前記第2部よりも低く、前記液滴生成装置用スロットは、前記第1部に形成され、前記動力発生装置用スロットは、前記第2部に形成されていることを特徴する請求項2に記載のマイクロフルイディクスチップシステム。
- 前記収集瓶は前記液滴生成装置外に位置し、前記ベースには、前記収集瓶を収容して固定する収集瓶用スロットがさらに形成されていることを特徴する請求項2に記載のマイクロフルイディクスチップシステム。
- 前記収集瓶は、前記チップ本体内に収容されて固定され、前記チップ本体は、前記液滴生成装置用スロットに収容されて固定されていることを特徴する請求項2に記載のマイクロフルイディクスチップシステム。
- 前記マイクロフルイディクスチップシステムは、少なくとも一つの振動装置を含み、前記振動装置は前記液滴生成装置用スロット内に設けられ、前記分散相入口に対応し、分散相に振動を与えることを特徴する請求項2に記載のマイクロフルイディクスチップシステム。
- 前記マイクロフルイディクスチップシステムは、少なくとも一つの振動装置を含み、前記振動装置は前記チップ本体内に設けられ、前記分散相入口に対応し、分散相に振動を与えることを特徴する請求項2に記載のマイクロフルイディクスチップシステム。
- 前記動力発生装置は、操作部を含み、前記第1位置決め部材は、前記操作部の末端に形成されることを特徴する請求項2に記載のマイクロフルイディクスチップシステム。
- 前記動力発生装置は、注射装置であることを特徴とする請求項9記載のマイクロフルイディクスチップシステム。
- 前記動力発生装置は、前記接続装置を介して前記収集瓶に封止接続された負圧発生装置であることを特徴とする請求項2~10のいずれかに記載のマイクロフルイディクスチップシステム。
- 前記調製台は、固定台と、前記固定台に反転自在に連結され、前記液滴生成装置側に位置するダストカバーと、をさらに含み、前記ダストカバーは、前記液滴生成装置内へのダストの侵入を防止することを特徴とする請求項11に記載のマイクロフルイディクスチップシステム。
- 前記動力発生装置は、前記接続装置を介して前記連続相入口および前記分散相入口にそれぞれ封止接続された正圧発生装置であることを特徴とする請求項2に記載のマイクロフルイディクスチップシステム。
- 請求項2~10のいずれかに記載のマイクロフルイディクスチップシステムを用いて実施される液滴の調製方法であって、
ピペットガンまたはピペット管によって所定量の連続相および分散相をそれぞれ計量し、計量した前記連続相を前記連続相入口内に導入し、計量した前記分散相を前記分散相入口内に導入するステップと、
動力発生装置を起動して、収集瓶、接続装置、チップ本体内に圧力差を発生させて、圧力差は前記分散相および前記連続相を合流させ収集瓶内に液滴として流入させるステップと、を含む。 - 前記動力発生装置を起動させる前に、少なくとも1つの振動装置により前記分散相に振動を加えるステップをさらに含むことを特徴とする請求項14に記載の液滴の調製方法。
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