JP7171603B2 - 緩衝液交換用の試料プレート及び製造方法 - Google Patents
緩衝液交換用の試料プレート及び製造方法 Download PDFInfo
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/027—Nanofiltration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0851—Bottom walls
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0858—Side walls
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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Description
本特許出願は、2017年3月22日に出願の米国仮特許出願第62/474,757号の優先権を主張し、その開示の全体が参照により本明細書内に組み込まれている。
本明細書には、生体試料の調製のために緩衝液を交換する試料プレートおよびその方法が開示されている。この方法は、高い圧力を使用してプレート中の貯留部のフィルターを通して緩衝液を押込む緩衝液の自動交換を含むことができる。フィルターのこの高圧下での脱離を回避するように、フィルターの貯留部への封止性を補強するフィルター取付け領域が配置されてもよい。さらにこの試料プレートの製造方法についても開示する。
試料プレート
緩衝液交換方法
製造
例1:二重封止の試料プレートと市販のプレートとの比較試験
例2:緩衝液交換された高濃度試料中のタンパク質の回収
Claims (14)
- 生体試料から緩衝液を自動交換する試料プレートであって、
複数の貯留部を含み、
前記複数の貯留部のうちの各貯留部は、側壁、内側隆起部、熱封止および接着剤封止を含むフィルター取付け領域、ならびに前記熱封止および前記接着剤封止に対応するように前記貯留部の前記側壁および内側隆起部によって規定されたリング形の溝部を備え、
前記フィルター取付け領域に各貯留部に固定された周縁部を有するフィルターを含み、
前記フィルター取付け領域は、前記フィルターを挟んで30psig以上の圧力差では各貯留部への前記フィルター周縁部の固着を維持している、試料プレート。 - 前記フィルターを挟む前記圧力差は、45psig以上である、請求項1に記載の試料プレート。
- 前記フィルターを挟む前記圧力差は、60psig以上である、請求項1に記載の試料プレート。
- 前記フィルター周縁部は折畳み形状を有し、前記熱封止および前記接着剤封止は、前記フィルター周縁部をその折畳み形状で各貯留部に固定する、請求項1に記載の試料プレート。
- 前記接着剤封止は、前記貯留部の前記側壁および内側隆起部によって規定された溝部内に配置された接着剤を含む、請求項4に記載の試料プレート。
- 前記貯留部の前記内側隆起部の高さは0.38mmである、請求項5に記載の試料プレート。
- 生体試料から緩衝液を自動交換する方法であって、
複数の貯留部を含む試料プレートを圧力室内に配置することを含み、
前記複数の貯留部のうちの各貯留部は、フィルター、ならびに熱封止および接着剤封止を含むフィルター取付け領域、ならびに各貯留部の側壁および内側隆起部によって規定されたリング形の溝部を備え、
前記試料プレート上部の前記圧力室内の空間を加圧し、前記フィルターを挟んで30psig以上の圧力差を発生させて、前記生体試料を第1の緩衝液から分離することを含む、方法。 - 前記フィルターを挟む圧力差は、45psig以上である、請求項7に記載の方法。
- 前記フィルターを挟む圧力差は、60psig以上である、請求項7に記載の方法。
- さらに第2の緩衝液を前記複数の貯留部に加えることを含む、請求項7に記載の方法。
- 緩衝液を自動交換する試料プレートを製造する方法であって、
周縁部を有するフィルターを複数の貯留部内に装填することを含み、
前記複数の貯留部のうちの各貯留部は、側壁、内側隆起部、ならびに熱封止および接着剤封止に対応するように前記側壁および前記内側隆起部により規定されたリング形の溝部を含むフィルター取付け領域を含み、
前記フィルター周縁部を前記フィルター取付け領域に熱的に封止すること、および
前記フィルター周縁部を前記フィルター取付け領域に接着剤により封止することを含む、方法。 - 熱封止は、前記フィルター周縁部が各貯留部の前記内側隆起部に固定されるように、前記フィルター周縁部に熱および圧力を加えることを含む、請求項11に記載の方法。
- 前記フィルター周縁部は、前記内側隆起部に対して折畳まれる、請求項12に記載の方法。
- 前記接着剤による封止は、前記溝部を接着剤で充填し前記接着剤を硬化させて、フィルター周縁部を各貯留部の前記側壁にさらに固定することを含む、請求項11に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US201762474757P | 2017-03-22 | 2017-03-22 | |
US62/474,757 | 2017-03-22 | ||
PCT/US2018/023568 WO2018175584A1 (en) | 2017-03-22 | 2018-03-21 | Sample plates for buffer exchange and methods of manufacture |
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JP2020517234A JP2020517234A (ja) | 2020-06-18 |
JP7171603B2 true JP7171603B2 (ja) | 2022-11-15 |
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US (1) | US11891414B2 (ja) |
EP (1) | EP3600670A4 (ja) |
JP (1) | JP7171603B2 (ja) |
KR (1) | KR102599895B1 (ja) |
CN (1) | CN111093829B (ja) |
AU (1) | AU2018239436B2 (ja) |
CA (1) | CA3056434C (ja) |
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US11891414B2 (en) | 2017-03-22 | 2024-02-06 | Unchained Labs | Sample plates for buffer exchange and methods of manufacture |
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JP2010036081A (ja) | 2008-08-04 | 2010-02-18 | Toray Ind Inc | 膜エレメントおよびその製造方法 |
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WO2015179598A2 (en) | 2014-05-21 | 2015-11-26 | Freeslate, Inc. | Systems and methods for exchange of buffer solutions |
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US11891414B2 (en) | 2017-03-22 | 2024-02-06 | Unchained Labs | Sample plates for buffer exchange and methods of manufacture |
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JP2003506456A (ja) | 1999-08-06 | 2003-02-18 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | マルチウェルにおける減圧濾過による自動タンパク質精製 |
JP2006181567A (ja) | 2004-10-18 | 2006-07-13 | Becton Dickinson & Co | 透析膜を備えたマイクロプレート |
JP2010036081A (ja) | 2008-08-04 | 2010-02-18 | Toray Ind Inc | 膜エレメントおよびその製造方法 |
US20140298924A1 (en) | 2010-12-23 | 2014-10-09 | Emd Millipore Corporation | Chromatography Apparatus And Method |
WO2015179598A2 (en) | 2014-05-21 | 2015-11-26 | Freeslate, Inc. | Systems and methods for exchange of buffer solutions |
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AU2018239436B2 (en) | 2022-12-15 |
US20180273583A1 (en) | 2018-09-27 |
CN111093829B (zh) | 2022-09-27 |
CA3056434C (en) | 2023-09-05 |
WO2018175584A1 (en) | 2018-09-27 |
CN111093829A (zh) | 2020-05-01 |
JP2020517234A (ja) | 2020-06-18 |
CA3056434A1 (en) | 2018-09-27 |
EP3600670A4 (en) | 2020-10-28 |
KR20200019599A (ko) | 2020-02-24 |
KR102599895B1 (ko) | 2023-11-07 |
AU2018239436A1 (en) | 2019-10-03 |
EP3600670A1 (en) | 2020-02-05 |
US11891414B2 (en) | 2024-02-06 |
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