JP7156684B2 - oral composition - Google Patents

Description

The present invention relates to oral compositions containing kale.

BACKGROUND ART In recent years, against the background of increasing interest in health, various health foods have been developed, and various materials used in health foods have been studied.

For example, kale is one of the cruciferous plants like cabbage, and is rich in vitamins such as vitamin C, minerals, dietary fiber, chlorophyll, and the like. It is attracting attention as a health material suitable for maintaining the health of the human body, such as being effective for the health of the human body (see, for example, Patent Document 1).

In addition, in recent years, as safety consciousness is high as well as health consciousness, foods using safe ingredients such as kale, which is eaten on a daily basis, are preferred. It is desired to make more use of the useful functions that it has.

JP-A-2002-171932

An object of the present invention is to provide a kale-containing oral composition having useful functions.

The present inventors have found that the use of proteoglycan together with kale provides an excellent fibroblast-protecting effect (fibroblast-activating effect), and have completed the present invention. In addition, the inventors have found that dispersibility is improved by using a specific material such as proteoglycan together with kale, and have completed the present invention.

That is, the present invention is as follows.
[1] An oral composition comprising kale and proteoglycan.
[2] The oral composition according to [1] above, which further contains at least one material selected from placenta, astaxanthin, collagen, hyaluronic acid and matcha green tea.

The oral composition of the present invention has excellent fibroblast protective action. Moreover, the oral composition of the present invention has high dispersibility and can suppress the formation of lumps.

FIG. 2 is a diagram showing the results of cytotoxicity suppression rate (fibroblast activation rate) due to oxidative stress when the composition of the present invention was applied to human dermal fibroblasts (NHDF). 1 is a graph showing the results of a dispersion test for compositions of the present invention;

The oral composition of the present invention is characterized by containing kale and proteoglycan. The oral composition of the present invention preferably further contains at least one material selected from placenta, astaxanthin, collagen, hyaluronic acid and green tea (hereinafter sometimes referred to as other materials including proteoglycan).

The oral composition of the present invention has excellent fibroblast protective action. That is, the oral composition of the present invention can protect fibroblasts that contribute to the production of collagen and hyaluronic acid, protect the skin from damage such as ultraviolet rays, and prevent wrinkles and aging (anti-aging). You can get the beauty effect of

Moreover, the oral composition of the present invention containing kale and other ingredients has higher dispersibility than kale. That is, the oral composition of the present invention is a powder (powder) that does not easily aggregate. Therefore, when dissolved in a liquid such as water or hot water and ingested, it dissolves easily and can be easily mixed. Furthermore, in the manufacturing process of the product, it is possible to suppress the adhesion of the powders in the powder mixing process, suppress the formation of lumps, and achieve uniform mixing. That is, the highly dispersible oral composition of the present invention can be prepared simply by mixing, and the preparation is also easy. For example, when mixing raw materials that are highly agglomerated and tend to form clumps, the raw materials are subjected to pretreatment such as pulverization using a pulverizer to ensure the fluidity of the raw materials. It is known that after charging, it is necessary to supply air and start flowing as quickly as possible to prevent reaggregation, but there is no need to perform such complicated operations. It is also known that the adhesiveness can be reduced by pre-mixing a raw material that is highly cohesive and tends to form lumps with an excipient, but such addition is not necessary.

In addition, since the composition of the present invention has high dispersibility, even when materials other than the components of the present invention are mixed to form the oral composition of the present invention, it is not necessary to perform the above-described complicated operations. can be prepared by simply mixing.

[Kale]
Kale is a cruciferous plant. The variety of kale used in the present invention is not particularly limited, and various kinds of kale such as kitchen kale, tree kale, bush kale, mallow kale, collard greens, and green leaf kale can be used. It is preferable that the kale is processed, such as drying and pulverizing, immediately after harvesting. If it takes a long time to process, it is preferable to store the kale by a storage means commonly used by those skilled in the art, such as low-temperature storage, in order to prevent deterioration of the kale. The site used in the present invention is preferably leaves, and among leaves, green leaves are preferred. Moreover, the stem may be included together with the leaves. In addition, kale may be mixed with other green juice materials such as young barley grass, kumazasa, angelica keiskei, sweet potato, and mugwort.

Kale may be a processed product obtained by processing kale, for example, it can be used as a pulverized product, juice, extract, or the like. Examples of the pulverized material include dry powder, shredded material, and dried material thereof (dried shredded material). The squeezed juice or extract may be in liquid form, but may also be used in the form of paste or dry powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of solvents that can be used include water (warm water, hot water), ethanol, and hydrous ethanol. In the present invention, a dry powder obtained by drying and pulverizing kale is preferable because the nutrients contained in kale can be effectively utilized. The dry powder preferably has a particle size of 75 μm or less in 60% or more, more preferably 75% or more, and particularly preferably 90% or more.

[Other materials]
(proteoglycan)
Proteoglycans are complex polysaccharides in which glycosaminoglycans such as chondroitin sulfate and dermatan sulfate are covalently bound to a protein core, and are abundant in animal tissues, particularly cartilage tissues. It is also known that proteoglycan exists in vivo in a structure in which the core protein is further bound to hyaluronic acid, and its molecular weight is as large as tens of thousands to tens of millions. Proteoglycans used in the present invention specifically include animal-derived proteoglycans, and salmon nasal cartilage-derived proteoglycans are particularly preferred. Moreover, a commercial item can be used. In the present invention, a powdery one is preferable, and when combined with kale, a particularly excellent dispersibility improving effect is exhibited, so it is preferable that 80% or more of the particle size passes through 40 mesh. It is more preferably 90% or more, and particularly preferably 100%.

(Placenta)
Placenta extracts derived from mammals such as humans, pigs and horses and fish such as salmon can be used as the placenta, and placenta extracts derived from salmon ovary membranes are preferred. A conventionally known method can be used for the extraction method, and various forms such as liquid, powder, solid, and granules can be used. In the present invention, a powdery one is preferable, and when combined with kale and proteoglycan, it exhibits a particularly excellent dispersibility improving effect, so it is recommended that 80% or more of the particle size pass through 40 mesh. It is preferably 90% or more, more preferably 100%.

(astaxanthin)
Astaxanthin used in the present invention is a kind of carotenoid and is said to have 1000 times more antioxidant action than vitamin E. It may be derived from natural products or obtained synthetically. Examples of those derived from natural products include astaxanthin obtained from algae such as Haematococcus algae, crustaceans such as shrimps and crabs, and fish such as salmon and sea bream, and in particular, astaxanthin obtained from Haematococcus algae. Astaxane is preferred. Extracts derived from natural products and chemically synthesized products are commercially available, and these commercially available products can be used. In the present invention, a powdery one is preferable, and when combined with kale and proteoglycan, it exhibits a particularly excellent dispersibility improving effect, so it is recommended that 80% or more of the particle size pass through 30 mesh. It is preferably 90% or more, more preferably 100%.

(collagen)
Collagen may be animal-derived collagen or synthetic collagen. In addition to collagen protein, collagen peptide obtained by hydrolyzing collagen protein, and telopeptide portion obtained by treating collagen molecule with protease. contains atelocollagen with the As the animal-derived collagen, fish-derived collagen is particularly preferable. Although the average molecular weight (weight average molecular weight) of collagen is not particularly limited, it is preferably from 500 to 100,000, more preferably from 1,000 to 50,000. In the present invention, a powdery one is preferable, and when combined with kale and proteoglycan, it exhibits a particularly excellent dispersibility improving effect, so it is recommended that 80% or more of the particle size pass through 40 mesh. It is preferably 90% or more, more preferably 100%.

(hyaluronic acid)
Examples of hyaluronic acid that can be used include those extracted from animal tissue such as chicken combs and those produced by microorganisms such as lactic acid bacteria. Rooster's comb has been used as food for a long time, and there is no problem in terms of safety. Hyaluronic acid may also be in the form of a salt. Hyaluronic acid salts include sodium hyaluronate and potassium hyaluronate.

(Matcha)
Matcha is a type of green tea derived from plants belonging to the Camellia family and the genus Camellia, and is powdered green tea such as tencha, sencha, and gyokuro. In the present invention, commercially available products may be used, and those produced by methods known in the art may also be used. In the present invention, a powdery one is preferred, and when combined with kale and proteoglycan, a particularly excellent dispersibility improving effect is exhibited. It is more preferably 90% or more, and particularly preferably 100%.

The oral composition of the present invention, in addition to general foods, food additives, feeds, etc., has a skin-beautifying effect based on fibroblast-protecting action, so it can be used, for example, as a food for specified health uses, a food with nutrient function claims, and It can be used as a so-called health food such as a functional food whose indication of efficacy has been approved by a designated organization such as a food, or as a drug (including quasi-drugs).

That is, the oral composition of the present invention can be used as a skin-beautifying oral composition used for skin beauty such as so-called health foods and pharmaceuticals, and such a skin-beautifying oral composition contains kale and proteoglycan. , In terms of being used for skin care, the product is not particularly limited as long as it can be distinguished from other products. The scope of the present invention includes those labeled as having skin beauty functions such as skin protection effect, skin barrier function improvement/enhancement effect, skin turnover promotion effect, and fibroblast activation effect. The skin-beautifying oral composition of the present invention is not limited to one in which the ingredients of the present invention (kale and proteoglycan) are indicated as active ingredients for skin care on the product packaging or the like. For example, an active ingredient may not be specified, or a specified material may be indicated as an active ingredient.

Specifically, in the so-called health food, "barrier the skin", "protect the skin", "enhance the barrier function of the skin", "enhance the moisturizing power of the skin", and "for those who are concerned about spots". , "for wrinkles", "for stress-resistant skin", "for UV-resistant skin", "good for beauty", "good for skin", etc. can. The oral composition of the present invention can be ingested by a person concerned about the above effects regardless of gender or age. It is preferably taken by women.

Forms of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. shape, gel shape, chewable shape, stick shape, and the like. Among these, tablets, capsules, powders, granules and liquids are particularly preferred. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant beverages (powder beverages) for dissolving in water (hot water), milk, fruit juice, green juice, etc. , food additives can be exemplified. These are preferable in that they are easy to drink with meals and can improve palatability.

The content of kale and other materials (ingredients of the present invention) in the oral composition of the present invention may be appropriately contained within the range in which the effects thereof are exhibited.

In general, when the oral composition of the present invention is a supplement or pharmaceutical in the form of a tablet, tablets, etc., the component of the present invention is contained in an amount of 0.01 to 100% by mass in terms of dry mass of the entire composition. It is preferably contained in an amount of 0.1 to 80% by mass, more preferably 1 to 60% by mass.

When the oral composition of the present invention is a packaged beverage, the component of the present invention is preferably contained in an amount of 0.001 to 30% by mass, preferably 0.01 to 20% by mass of the total composition. more preferably 0.1 to 15% by mass.

Further, when the oral composition of the present invention is an instant beverage such as green juice, the component of the present invention preferably contains 0.01 to 100% by mass of the entire composition in terms of dry mass. It is more preferably contained in an amount of 1 to 80% by mass, more preferably 1 to 60% by mass.

In order to exhibit the effect of the present invention more effectively, the component of the present invention preferably accounts for 70% or more of the total oral composition of the present invention in terms of dry mass, and contains 80% or more. is more preferable, more preferably 90% or more, and particularly preferably 100%.

The intake of the oral composition of the present invention is not particularly limited, but from the viewpoint of exhibiting the effects of the present invention more remarkably, the intake of kale per day for adults is 0.01 g/day or more in terms of dry mass. preferably 0.1 g/day or more, and even more preferably 1 g/day or more. The upper limit is, for example, 30 g/day, preferably 20 g/day, more preferably 10 g/day. The oral composition of the present invention can be contained in one container or divided into a plurality of containers, for example 2 to 3, so that the daily intake is equal to the above-mentioned intake for one day.

The blending mass ratio of kale and other materials is preferably 0.0001:1 to 10000:1, more preferably 0.001:1 to 1000:1, more preferably 0.01 in terms of dry mass. :1 to 100:1 is more preferable.

The oral composition of the present invention can be produced by a known method by adding other ingredients other than the ingredients of the present invention, if necessary. Other ingredients other than the ingredients of the present invention include, for example, water-soluble vitamins (vitamin B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P), oil-soluble vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium, phosphorus, and iron; sulfur-containing compounds contained in taurine, garlic, etc.; hesperidin, quercetin, etc. proteins such as collagen; peptides; amino acids; animal oils and fats; vegetable oils and fats;

The oral composition of the present invention exhibits the fibroblast-protecting action shown in the examples described later, thereby providing a composition for protecting fibroblasts, a composition for activating fibroblasts, and a composition for improving/improving skin barrier function. It can be excellent as a product, a composition for promoting skin turnover, and a composition for beautiful skin. In addition, by exhibiting the effect of improving dispersibility shown in Examples described later, a composition that is less likely to aggregate and has high dispersibility can be obtained.

The present invention will be described below based on examples.
[Example 1]
(material)
As the kale, dried kale powder (90% or more with a particle size of 75 μm or less; produced by Toyo Shinyaku Co., Ltd.) was used.
As the proteoglycan, dry powder of proteoglycan extracted from salmon nasal cartilage (90% or more passing through 40 mesh) was used.
As the placenta, dried placenta powder (90% or more passing through 40 mesh) extracted and purified from salmon ovary membrane was used.
As the astaxanthin, dry powder of astaxanthin extracted from Haematococcus algae (30 mesh pass is 90% or more; astaxanthin content is 2.0% or more) was used.
As the collagen, dry powder (90% or more passing through 40 mesh) of collagen peptide (average molecular weight: 2000 to 2500) purified from marine fish scales was used.
Sodium hyaluronate powder was used as hyaluronic acid.
As the matcha, dried matcha powder (90% or more with a particle size of 75 μm or less; manufactured by Toyo Shinyaku Co., Ltd.), which is obtained by drying and pulverizing tencha tea leaves, was used.

[Test Example 1]
[Test for suppressing cytotoxicity due to oxidative stress (confirmation test for cell activation activity)]
(Preparation of sample solution)
<Kale>
Kale dry powder was suspended in DMEM medium containing 0.5% DMSO and 10% FBS (hereinafter referred to as additive medium) to a concentration of 20 mg/mL, and stirred for 10 minutes using a vortex mixer. The centrifugation supernatant was sterilized through a 0.2 μm filter and diluted with an additive medium to a concentration of 1 mg/mL to prepare a sample solution.

<Proteoglycan>
A sample solution was prepared in the same manner as for kale, except that proteoglycan dry powder was used.

<Astaxanthin>
Astaxanthin dry powder was suspended in DMEM medium containing 25% DMSO and 10% FBS to a concentration of 200 mg/mL. A concentration of 4 mg/mL was prepared in DMEM medium containing 10% FBS. The centrifugation supernatant was sterilized through a 0.2 μm filter and diluted with an additive medium to a concentration of 1 mg/mL to prepare a sample solution.

<Kale + Proteoglycan>
It was prepared by mixing equal amounts of each sample solution adjusted to a concentration of 1 mg/mL.
<Kale + Proteoglycan + Astaxanthin>
It was prepared by mixing equal amounts of each sample solution adjusted to a concentration of 1 mg/mL.
<Control>
The additive medium itself was used as a sample solution.

(test)
Human dermal fibroblasts (NHDF) were cultured in DMEM containing 10% FBS using a 75 cm ² flask in a 37° C., 5% CO ₂ incubator.
Cells suspended by trypsinization were seeded from a 75 cm ² flask into each well of a collagen-coated 96-well plate at a cell density of 10,000 cells/well.
After culturing for 24 hours in a 37° C., 5% CO ₂ incubator, the culture supernatant was removed, and 200 μL of each sample solution adjusted to a concentration of 1 mg/mL was added and cultured for 24 hours.
After culturing for 24 hours, hydrogen peroxide was added to the cells to a concentration of 100 μM, and the cells were cultured in a 37° C., 5% CO ₂ incubator for 90 minutes.

As a method for adding hydrogen peroxide, 50 μL / well of hydrogen peroxide adjusted to a concentration of 500 μM in the medium for addition is added to the medium containing the test substance cultured at 200 μL / well. The final concentration of hydrogen was 100 μM.
After adding hydrogen peroxide and culturing the cells for 90 minutes, the cells were washed once with 300 μL/well of PBS, and 150 μL/well of Cell Counting Kit-8 solution (manufactured by Dojindo Laboratories) diluted 30-fold with serum-free DMEM medium. Added in wells.

After standing in a 37° C., 5% CO ₂ incubator for proper color development, the absorbance of each well at 450 nm was measured.
Based on the obtained data, the rate of inhibition of oxidative stress-induced damage to fibroblasts (cell activation activity) (% of control) was calculated according to the following formula.

% of control = (Data sample - Data blank) / (Data control - Data blank) x 100

In the formula, "Data sample" represents the absorbance of each test substance-added well, "Data control" represents the absorbance of control wells, and "Data blank" represents the absorbance of wells without cells.

The results are shown in FIG. As shown in FIG. 1, the composition of the present invention (kale + proteoglycan) synergistically improves the rate of inhibition of fibroblast damage (cell activation activity) due to oxidative stress. The composition of the invention (kale + proteoglycan + astaxanthin) showed significant improvement. Therefore, according to the oral composition of the present invention, fibroblasts are protected and a high skin-beautifying effect can be obtained.

[Test Example 2]
(Dispersibility test)
Each raw material was sieved (nominal size: 710). When two or more raw materials were combined, the amount required for measuring the degree of dispersion was weighed according to the proportions shown in Table 1, and mixed in a plastic bag by shaking by hand until uniform.

10 g of the test substance was weighed and the degree of dispersion was measured using a powder specific evaluation device "Powder Tester PT-X" manufactured by Hosokawa Micron Corporation. The measurement of dispersity was performed according to the manual of the instrument.

In addition, the degree of dispersion is measured by the following method.
A sample having a specified weight (W) is dropped from a specified height onto a tray, and the weight (W1) of the sample placed on the tray is measured. Furthermore, the degree of dispersion is calculated by the following formula.

Dispersion degree (%) = (W - W1) / W x 100

Table 1 and FIG. 2 show the composition of each test substance and the results thereof.

As shown in Table 1 and FIG. 2, the composition of the present invention containing other materials together with kale and proteoglycan has a high degree of dispersion and improved dispersibility compared to kale alone (Comparative Example 1). did. Also, the effect of dispersibility was synergistic.

[Formulation example]
Powdered compositions for food and drink (oral compositions of the present invention) shown in Table 2 were prepared. When 3 g of the obtained composition of the present invention was mixed with 150 mL of water and drunk, the smell and taste of kale were improved, and a beverage with excellent overall palatability was obtained.

The oral composition of the present invention is industrially useful because it can be used as a so-called health food or the like.

Claims (4)

An oral composition comprising kale, proteoglycan and astaxanthin . 2. The oral composition according to claim 1, further comprising at least one material selected from placenta , collagen , hyaluronic acid and green tea. An oral composition comprising kale, proteoglycan and placenta (with the exception of apple, pear, grapefruit, passion fruit, lemon, red grape, strawberry, pineapple, mango, lychee, orange, kiwifruit, peach , blueberries, cranberries and raspberries, and green juices containing barley grass, mulberry leaves, kumazasa, Angelica keiskei, kale and matcha green tea) . An oral composition comprising kale, proteoglycans and matcha green tea (except green juice containing young barley grass, mulberry leaves, kumazasa, angelica keiskei, kale and green tea) .

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