JP7385256B2 - Oral composition - Google Patents

Description

The present invention relates to oral compositions containing kale.

In recent years, against the background of increasing interest in health, various health foods have been developed, and various materials used in health foods are being researched.

For example, kale is a member of the cruciferous family like cabbage, and is rich in vitamins such as vitamin C, minerals, dietary fiber, and chlorophyll, and is also useful for preventing gastritis and stomach ulcers, improving liver function, and constipation. It is attracting attention as a health material suitable for maintaining the health of the human body, such as being effective for the health of the human body (see, for example, Patent Document 1).

In addition, these days, people are as conscious about safety as they are about health, and people prefer foods made from safe ingredients like kale, which is eaten on a daily basis. It is hoped that they will be utilized more.

Japanese Patent Application Publication No. 2002-171932

An object of the present invention is to provide a kale-containing oral composition that has the effect of maintaining and enhancing muscle tissue.

The present inventors have discovered that an excellent myoblast proliferation effect can be obtained by using a specific material (other materials) together with kale, and have completed the present invention.

That is, the present invention is as follows.
[1] An oral composition containing kale and at least one other material selected from protein, gymnema, sucralose, mulberry, and kumama.
[2] The oral composition according to [1] above, which contains at least two other materials.
[3] The oral composition according to [1] or [2] above, which is used for dieting.
[4] The oral composition according to any one of [1] to [3] above, which comprises dry powder of kale.
[5] The oral composition according to [4] above, wherein the dry powder of kale is a dry powder obtained by crushing and powdering dry green leaves of kale.

The oral composition of the present invention has excellent muscle tissue maintenance and strengthening effects.

1 is a graph showing the results of a dispersion test of the composition of the present invention (kale+kumzaza (+other materials other than kumaza)). It is a graph showing the results of a dispersion test of the composition of the present invention (kale + mulberry leaves (+ other materials other than mulberry leaves)). It is a graph showing the results of a dispersion test of the composition of the present invention (kale + protein (+ other materials other than protein)). It is a graph showing the results of a dispersion test of the composition of the present invention (kale+gymnema (+other materials other than gymnema)). It is a graph showing the results of a dispersion test of the composition of the present invention (kale+sucralose (+other materials other than sucralose)).

The oral composition of the present invention is characterized by containing kale and at least one other material selected from protein, gymnema, sucralose, mulberry, and kumamaza.

The oral composition of the present invention has an excellent myoblast proliferation effect and can maintain and strengthen muscle tissue. In addition, since muscle tissue is a tissue that actively metabolizes energy, by maintaining and strengthening this muscle tissue, basal metabolic rate can be maintained and increased, and in turn, a diet effect can be obtained by preventing and/or eliminating obesity. Can be done.

Furthermore, the oral composition containing the kale of the present invention and at least one other material has higher dispersibility than kale alone. That is, the oral composition of the present invention is a powder that does not easily aggregate. This highly dispersible oral composition can be prepared simply by mixing, and is easy to prepare. For example, when mixing raw materials that are highly cohesive and tend to form lumps, the raw materials are pretreated such as crushing using a crusher to ensure fluidity of the raw materials before being mixed. It is known that after charging, it is necessary to supply air and start flow as quickly as possible to prevent reagglomeration, but there is no need to perform such complicated operations. It is also known that adhesion can sometimes be alleviated by pre-mixing excipients with raw materials that are highly cohesive and tend to form lumps, but such addition is not necessary.

Furthermore, since the composition of the present invention containing kale and at least one other material has high dispersibility, even when materials other than kale and other materials are mixed to form the oral composition of the present invention, There is no need to perform complicated operations such as those described above, and the preparation can be performed simply by mixing.

[Kale]
Kale is a plant in the cruciferous family. The variety of kale used in the present invention is not particularly limited, and various types of kale such as kitchen kale, tree kale, bush kale, mallow kale, collard, and green-leafed kale can be used. It is preferable that the kale be processed immediately after harvesting, such as drying and pulverizing. If it takes time to process, it is preferable to store the kale by storage means commonly used by those skilled in the art, such as low temperature storage, to prevent deterioration of the quality of the kale. The site used in the present invention is preferably a leaf, and among leaves, green leaves are preferred. Moreover, the stem may be included together with the leaves.

Kale can be used as a crushed product, juice, extract, etc. Examples of the pulverized product include a dry powder, a finely divided product, and a dried product thereof (dried finely divided product). The squeezed juice or extract may be in liquid form, but it can also be used in the form of a paste or dry powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent that can be used include water (warm water, hot water), ethanol, and aqueous ethanol. As for kale, since the nutritional components contained in kale can be effectively utilized, dried green leaves of kale are preferable, and fragmented products and dried products thereof (dried fragmented products) are more preferable, and dry powder obtained by pulverizing and pulverizing. is particularly preferred. Moreover, it is preferable that the dry powder of kale has a particle size of 75 μm or less in a proportion of 60% or more, more preferably 75% or more, and particularly preferably 90% or more.

[Other materials]
The other material used together with kale is at least one selected from protein, gymnema, sucralose, mulberry, and kumama, and it is preferable to use a combination of at least two of these other materials. When combining two or more other materials, a combination of protein and mulberry, or a combination of gymnema and sucralose is particularly preferred. That is, oral compositions containing kale, protein, and mulberry, and oral compositions containing kale, gymnema, and sucralose are preferred because they exhibit particularly excellent myoblast proliferation effects due to the synergistic action of the combination. , particularly excellent diet (anti-obesity) effects can be expected.

(protein)
Protein is extracted and purified protein contained in animals and plants, and commercially available products can be used. In the present invention, powdered materials are preferred. Examples of animal-derived proteins include whey protein, casein protein, egg protein, beef protein, etc.; examples of plant-derived proteins include soy protein, pea protein, rice protein, etc. . In the present invention, it is preferable to use protein derived from plants, and it is particularly preferable to use protein derived from soybeans (soy protein) because it exhibits a particularly excellent myoblast proliferation effect when combined with kale. More preferred. In addition, soy protein has a protein content of preferably 50% or more, preferably 70% or more, and 90% or more in terms of dry matter, since it exhibits a particularly excellent dispersibility-improving effect when combined with kale. Particularly preferred.

(gymnema)
Gymnema is a climbing plant that belongs to the Apocynaceae family and is native to India and Sri Lanka, such as Gymnema sylvestre. As the site used in the present invention, leaves are preferred. Moreover, the stem may be included together with the leaves. Gymnema can be used as a ground product, squeezed product, extract, etc. Examples of the pulverized product include a dry powder, a finely divided product, and a dried product thereof (dried finely divided product). The squeezed juice or extract may be in liquid form, but it can also be used in the form of a paste or dry powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent that can be used include water (hot water), ethanol, and aqueous ethanol. In the present invention, dry powder of Gymnema is preferable, and it is more preferable to use a dry powder of hydroalcoholic extract of Gymnema leaves because it exhibits a particularly excellent myoblast proliferation effect when combined with kale. . In addition, since the dried powder of gymnema leaves exhibits a particularly excellent dispersibility improving effect when combined with kale, the gymnematic acid content is preferably 5 to 80%, and preferably 10 to 60%. is more preferable, and particularly preferably 20 to 40%.

(Sucralose)
Sucralose is a highly safe, non-caloric, high-intensity sweetener that is manufactured from sucrose and has a sweetness approximately 600 times that of sucrose. In the present invention, various forms such as liquid, powder, and solid can be used, and commercially available products can be used. In the present invention, a powder is preferable, and when combined with kale, it exhibits a particularly excellent effect of improving dispersibility. Therefore, it is preferable that 80% or more of the particle size is 20 mesh or less, and 90 % or more, and particularly preferably 99% or more.

(mulberry)
Mulberry is a plant of the Moraceae family. The site used in the present invention is preferably a leaf, and among leaves, green leaves are preferred. Moreover, the stem may be included together with the leaves. Mulberry can be used as a crushed product, squeezed juice, extract, etc. Examples of the pulverized product include a dry powder, a finely divided product, and a dried product thereof (dried finely divided product). The squeezed juice or extract may be in liquid form, but it can also be used in the form of a paste or dry powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent that can be used include water (hot water), ethanol, and aqueous ethanol. In the present invention, dry powder of mulberry is preferable, and since it exhibits a particularly excellent proliferative effect on myoblasts when combined with kale, it is more preferable to use dry powder obtained by crushing dry green leaves of mulberry. preferable. In addition, when combined with kale, it exhibits a particularly excellent dispersibility improving effect, so it is preferable that 60% or more of the dry mulberry powder has a particle size of 75 μm or less, and preferably 75% or more. More preferably, it is particularly preferably 90% or more.

(Kumazasa)
Kumazasa is a plant belonging to the Poaceae family. The site used in the present invention is preferably a leaf, and among leaves, green leaves are preferred. Moreover, the stem may be included together with the leaves. Kumazasa can be used as a crushed product, squeezed juice, extract, etc. Examples of the pulverized product include a dry powder, a finely divided product, and a dried product thereof (dried finely divided product). The squeezed juice or extract may be in liquid form, but it can also be used in the form of a paste or dry powder (squeezed juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent that can be used include water (hot water), ethanol, and aqueous ethanol. In the present invention, dry powder of Kumzasa is preferable, and since it exhibits a particularly excellent myoblast proliferation effect when combined with kale, it is more preferable to use a dry powder obtained by crushing and powdering the dried green leaves of Kumzasa. preferable. In addition, when combined with kale, it exhibits a particularly excellent dispersibility-improving effect, so it is preferable that the dry powder of Kumazasa has 50% or more of 350 mesh or less, and more preferably 75% or more. Preferably, it is particularly preferably 90% or more.

In addition to general foods, food additives, feeds, etc., the oral composition of the present invention has a dieting (anti-obesity) effect based on the excellent myoblast proliferation effect, so it can be used, for example, as a food for specified health uses, It can be used as a so-called health food such as a functional food whose efficacy has been approved by a designated organization such as a nutritionally functional food or a food with functional claims, or as a medicine (including quasi-drugs).

That is, the oral composition of the present invention can be used as a dieting oral composition used for dieting so-called health foods, medicines, etc.; The product is not particularly limited as long as it contains the following ingredients and can be distinguished from other products in that it can be used for dieting; for example, the main body, packaging, and instructions of the product according to the present invention. The scope of the present invention includes advertising materials that display the fact that they have a diet function. Note that the oral composition for dieting of the present invention is not limited to one in which the combined ingredients (kale and other ingredients) of the present invention are indicated as active ingredients for dieting on the product packaging or the like. For example, the active ingredient may not be specified, or only kale may be displayed as the active ingredient.

Specifically, so-called health foods are ``for people who are concerned about their weight,'' ``increase metabolism,'' ``helping obese people reduce body fat,'' and ``increasing the ability to metabolize fat.'' , "Supports styles", etc. can be exemplified.

Examples of the form of the composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. , gel form, chewable form, stick form, etc. Among these, tablet, capsule, powder, granule, and liquid forms are particularly preferred. Specifically, these include supplements, packaged beverages filled in plastic bottles, cans, bottles, etc., instant beverages (powdered beverages) that can be dissolved in water (hot water), milk, fruit juice, green juice, etc. , food additives can be exemplified. These are preferable because they are easy to drink during meals and can enhance palatability.

The content of kale and other materials (components of the present invention) in the oral composition of the present invention may be appropriately contained within the range where their effects are achieved.

Generally, when the oral composition of the present invention is a supplement or medicine in tablet form, the ingredient of the present invention is contained in an amount of 0.01 to 100% by mass of the entire composition in terms of dry mass. The content is preferably from 0.1 to 80% by mass, and even more preferably from 1 to 60% by mass.

When the oral composition of the present invention is a packaged beverage, the component of the present invention is preferably contained in an amount of 0.001 to 30% by mass, and preferably 0.01 to 20% by mass of the entire composition. It is more preferable that the content is 0.1 to 15% by mass.

Furthermore, when the oral composition of the present invention is an instant beverage such as green juice, it is preferable that the component of the present invention is contained in an amount of 0.01 to 100% by mass of the entire composition in terms of dry mass; The content is more preferably 1 to 80% by mass, and even more preferably 1 to 60% by mass.

In order to more effectively exhibit the effects of the present invention, it is preferable that the ingredients of the present invention are contained in an amount of 70% or more, and preferably 80% or more, of the entire oral composition of the present invention in terms of dry mass. It is more preferable that the content is 90% or more, and it is particularly preferable that the content is 100%.

Although there is no particular restriction on the amount of oral composition of the present invention to be ingested, from the viewpoint of more significantly exerting the effects of the present invention, the daily intake of kale for adults is 0.01 g/day or more in terms of dry mass. It is preferable to take in an amount of 0.1 g/day or more, more preferably 0.1 g/day or more, and even more preferably 1 g/day or more. The upper limit is, for example, 30 g/day, preferably 20 g/day, and more preferably 10 g/day. The oral composition of the present invention can be stored in one container or divided into a plurality of containers, for example, 2 to 3, so that the daily intake amount is the above-mentioned intake amount.

The blending mass ratio of kale and other materials is preferably 0.0001:1 to 10000:1, more preferably 0.001:1 to 1000:1, and 0.01 in terms of dry mass. More preferably, the ratio is from 1 to 100:1.

The oral composition of the present invention can be manufactured by a known method by adding other components other than the components of the present invention, if necessary. Other ingredients other than the ingredients of the present invention include, for example, water-soluble vitamins (vitamins B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, Vitamins such as vitamin P) and oil-soluble vitamins (vitamins A, D, E, K); Minerals such as calcium, magnesium, phosphorus, and iron; Sulfur-containing compounds contained in taurine, garlic, etc.; Hesperidin, quercetin, etc. Examples include flavanoids or flavonoids; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils; ground products or extracts of animals and plants (other than kale and other materials of the present invention).

The oral composition of the present invention has the effect of promoting the proliferation of myoblasts as shown in the Examples described below, and thus can be used as a composition for myoblast proliferation, a composition for muscle strengthening, a composition for increasing muscle mass, and a composition for increasing muscle mass. It can be excellent as a metabolic activator, a composition for dieting, a composition for reducing adipose tissue, and a composition for reducing body fat. Furthermore, by exhibiting the effect of improving dispersibility as shown in the Examples described later, it can be made into an excellent oral composition that is resistant to agglomeration and has high dispersibility.

The present invention will be explained below based on Examples.
[Example 1]
(material)
As the kale, dried kale powder (90% or more having a particle size of 75 μm or less; manufactured by Toyo Shinyaku Co., Ltd.) obtained by drying and pulverizing kale leaves was used.
As the Kumzaza, Kumzaza dry powder (90% or more of Kumzaza of 350 mesh or less; commercially available), which was obtained by drying and powdering Kumzaza leaves, was used.
As the mulberry leaves, dry mulberry leaf powder (90% or more of particles having a particle size of 75 μm or less; manufactured by Toyo Shinyaku Co., Ltd.) was used.
As protein, we use powdered isolated soy protein (protein content of 90% or more on dry matter basis; commercially available product) obtained by neutralizing the curd obtained by acid precipitation of soy milk extracted from defatted soybeans, then drying and passing through a 20-mesh sieve. ) was used.
As gymnema, Gymnema sylvestre leaves are extracted with aqueous ethanol, and the extract is dried and powdered. Gymnemic acid content 25-30%; commercially available product) was used. As the sucralose, sucralose powder (commercially available) obtained by passing powdered sucralose through a 20 mesh sieve was used.

These were mixed with raw materials at the test substance concentrations (μg/mL) shown in Table 1 and the mixing ratios shown in Table 2, and each test was conducted.

[Test Example 1]
(Cell proliferation test)
Mouse myoblasts (C2C12) were cultured with 10% FBS-DMEM in a 75 cm 2 flask at 37° C. in a 5% CO 2 incubator. Cells suspended by trypsin treatment were seeded from a 75 cm 2 flask into each well of a collagen-coated 96-well plate at a cell density of 8,000 cells per well.

After preincubation for 24 hours at 37°C in a 5% CO2 incubator, 100 μL of the test substance prepared in 10% FBS-DMEM was added to the wells.
Specifically, the test substance was prepared and added as follows.
First, the test substance was suspended in 10% FBS-DMEM to a concentration of 5 mg/ml, and filtered through a 0.2 μm filter. Next, this test substance suspension containing 5 mg/ml was further diluted with 10% FBS-DMEM and added to the wells to give the concentrations shown in Table 1.

After culturing for 24 hours, 100 μL of MTT solution (MTT 500 μg/ml in DMEM), which had been washed twice with PBS and filter sterilized, was added and cultured for an additional 3 hours. After washing twice with PBS, 100 μL of 0.04 HCl in isopropanol was added.

After confirming that the dye had eluted out of the cells, the absorbance (570 nm, 630 nm) was measured to determine the cell proliferation rate (% of control).

% of control = [SampleDate] / [ControlDate] x 100

Data = [(Sample570nm－Sample630nm)－(blank570nm－blank630nm)]

Sample570nm and blank570nm are absorbance values at 570 nm, and Sample630nm and blank630nm are absorbance values at 630 nm (turbidity).

The results are shown in Table 1.

As shown in Table 1, the composition of the present invention, which contains at least one other material together with kale, has a theoretical value (based on the blending ratio using the value of the comparative example) that would be expected if kale was blended alone. An increase in the cell proliferation rate was observed compared to the calculated value). In other words, a synergistic increase was observed due to the combination of kale and other ingredients. In particular, when two types of other materials are blended together with kale, the increase is remarkable compared to when one type of other material is blended, and among them, Example 7 containing kale, mulberry and protein, kale In Example 8 containing , gymnema, and sucralose, a particularly remarkable increase was observed, and an excellent dieting effect can be expected.

[Test Example 2]
(Dispersibility test)
Each raw material was passed through a sieve (nominal size: 710). When two or more raw materials were combined, the amount required for measuring the degree of dispersion was measured according to the proportions shown in Table 2, and mixed by hand in a plastic bag until uniform.

10 g of the test substance was weighed out, and the degree of dispersion was measured using a powder specific evaluation device "Powder Tester PT-X" manufactured by Hosokawa Micron. The degree of dispersion was measured according to the manual of the device.

Note that the degree of dispersion is measured by the following method.
A sample with a specified weight (W) is dropped from a specified height onto a receiving tray, and the weight (W1) of the sample on the receiving tray is measured. Furthermore, the degree of dispersion is calculated using the following formula.

Dispersity (%) = (W-W1)/W x 100

The composition and results of each test substance are shown in Table 2 and Figures 1 to 5.

As shown in Table 2, the compositions of the present invention containing kale and other materials had a higher degree of dispersion and improved dispersibility than kale alone. In addition, the dispersion was higher than the theoretical value of the dispersion (value calculated based on the blending ratio using the value of the comparative example) that would be expected if each material was blended alone, and the dispersibility was improved.

As shown in FIG. 1, the composition of the present invention in which kale and kumazaa are further blended with any other material other than kumazasa has three types of combinations (Example 14) in which kale and kumazaa are further blended with gymnema. A remarkable improvement in dispersibility was observed compared to the two types of combination (Example 9) in which kale and kumazana were blended. The three types of combinations (Examples 17, 18, and 19) in which kale and black lettuce were combined with one other material selected from mulberry leaves, protein, and sucralose had better dispersibility than the two combinations of kale and black lettuce. Although the value was lower, the dispersibility was improved compared to the ideal value.

As shown in Figure 2, the compositions of the present invention (Examples 15, 17, 20, and 21) in which kale and mulberry leaves were further blended with any other material other than mulberry leaves were mixed with kale and mulberry leaves. A remarkable improvement in dispersibility was observed compared to the combination of the two types (Example 10).

As shown in Figure 3, the composition of the present invention in which kale and protein are further blended with any other material other than protein is more effective than the two combinations containing kale and protein (Example 11). A remarkable improvement in dispersibility was observed in three combinations (Examples 15, 18, and 23) in which protein was further blended with one other material selected from mulberry leaf, kumazase, and sucralose. The three combinations of kale, protein, and gymnema (Example 22) had the same level of dispersibility as the two combinations of kale and protein (Example 11), but the dispersion was lower than the ideal value. There was an improvement in sexual performance.

As shown in FIG. 4, the composition of the present invention is one in which kale and gymnema are further blended with any other material other than gymnema; Further improvement in dispersibility was observed compared to the two combinations of kale and gymnema (Example 12). The compositions of the present invention (Examples 16, 20, and 22) in which kale and gymnema are further blended with one other material selected from sucralose, mulberry leaves, and protein are two types of compositions (Example 12) in which kale and gymnema are blended. ), the dispersibility was lower than the ideal value, but the dispersibility was improved compared to the ideal value.

As shown in Figure 5, the compositions of the present invention (Examples 16, 19, 21, 23) in which kale and sucralose are further blended with any other material other than sucralose are different from the two types in which kale and sucralose are blended. A remarkable improvement in dispersibility was observed compared to the combination (Example 13).

From the above, the composition of the present invention can be obtained by simply mixing other materials with kale, and can be obtained without complicated operations such as processing raw materials and supplying air during mixing. Turns out it's not needed.

[Formulation example]
A powdered edible composition (oral composition of the present invention) shown in Table 3 was prepared. When 3 g of the resulting composition of the present invention was mixed with 150 mL of water and drunk, a beverage with improved kale odor and taste and excellent overall palatability was obtained.

The oral composition of the present invention is industrially useful because it can be used as a so-called health food.

Claims (1)

An oral composition for maintaining and enhancing muscle tissue characterized by containing protein, kale, and mulberry leaves (with the exception of whey protein isolate (including milk components), palatinose, defatted soybeans, psyllium seed coat powder, and organic barley). Young leaf powder, calcium HMB, medium chain fatty acid triglyceride, inulin, purified fish oil containing DHA/EPA, matcha, apple juice, cream extract powder (contains milk ingredients), barley grass, kale, banana puree, broccoli, spore-forming lactic acid bacteria, Fermented grain extract (contains wheat), pumpkin, bok choy, parsley, carrot, celery, bitter melon, spinach, mulberry leaves, molokheiya, yeast (contains zinc), mugwort, tomato, yeast (contains copper)/flavoring, citric acid , tripotassium citrate, trisodium citrate, thickener (xanthan gum), vitamin C, sweetener (acesulfame K, sucralose), magnesium oxide, Quillaja extract, vitamin E, nicotinamide, calcium pantothenate, pyrophosphate. (excluding smoothies containing ferric iron, vitamin B1, vitamin B6, vitamin A, vitamin B2, folic acid, vitamin D, vitamin B12).

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