JP7142840B2 - 細胞含有ハイドロゲル体及びその製造方法 - Google Patents

細胞含有ハイドロゲル体及びその製造方法 Download PDF

Info

Publication number
JP7142840B2
JP7142840B2 JP2018094499A JP2018094499A JP7142840B2 JP 7142840 B2 JP7142840 B2 JP 7142840B2 JP 2018094499 A JP2018094499 A JP 2018094499A JP 2018094499 A JP2018094499 A JP 2018094499A JP 7142840 B2 JP7142840 B2 JP 7142840B2
Authority
JP
Japan
Prior art keywords
cell
hydrogel
cells
aggregate
droplet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018094499A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019198267A5 (enExample
JP2019198267A (ja
Inventor
淳二 福田
達斗 景山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yokohama National University NUC
Kanagawa Institute of Industrial Science and Technology
Original Assignee
Yokohama National University NUC
Kanagawa Institute of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yokohama National University NUC, Kanagawa Institute of Industrial Science and Technology filed Critical Yokohama National University NUC
Priority to JP2018094499A priority Critical patent/JP7142840B2/ja
Priority to US17/047,761 priority patent/US12312581B2/en
Priority to PCT/JP2019/016332 priority patent/WO2019220843A1/ja
Priority to EP19803757.4A priority patent/EP3795683A4/en
Publication of JP2019198267A publication Critical patent/JP2019198267A/ja
Publication of JP2019198267A5 publication Critical patent/JP2019198267A5/ja
Application granted granted Critical
Publication of JP7142840B2 publication Critical patent/JP7142840B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/098—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer formed in the presence of the enzymes or microbial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0666—Mesenchymal stem cells from hair follicles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/18—Materials or treatment for tissue regeneration for hair reconstruction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Dermatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2018094499A 2018-05-16 2018-05-16 細胞含有ハイドロゲル体及びその製造方法 Active JP7142840B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2018094499A JP7142840B2 (ja) 2018-05-16 2018-05-16 細胞含有ハイドロゲル体及びその製造方法
US17/047,761 US12312581B2 (en) 2018-05-16 2019-04-16 Cell-containing hydrogel body and method for producing same
PCT/JP2019/016332 WO2019220843A1 (ja) 2018-05-16 2019-04-16 細胞含有ハイドロゲル体及びその製造方法
EP19803757.4A EP3795683A4 (en) 2018-05-16 2019-04-16 CELLULATED HYDROGEL BODY AND PROCESS FOR THE PRODUCTION THEREOF

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2018094499A JP7142840B2 (ja) 2018-05-16 2018-05-16 細胞含有ハイドロゲル体及びその製造方法

Publications (3)

Publication Number Publication Date
JP2019198267A JP2019198267A (ja) 2019-11-21
JP2019198267A5 JP2019198267A5 (enExample) 2021-04-30
JP7142840B2 true JP7142840B2 (ja) 2022-09-28

Family

ID=68540166

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018094499A Active JP7142840B2 (ja) 2018-05-16 2018-05-16 細胞含有ハイドロゲル体及びその製造方法

Country Status (4)

Country Link
US (1) US12312581B2 (enExample)
EP (1) EP3795683A4 (enExample)
JP (1) JP7142840B2 (enExample)
WO (1) WO2019220843A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4130236A4 (en) * 2020-04-03 2025-07-16 Jsr Corp METHOD FOR PRODUCING CELL-CONTAINING EXTRACELLULAR MATRIX, CELL CULTURE METHOD, DEVICE FOR PRODUCING CELL-CONTAINING EXTRACELLULAR MATRIX, AND CONTROL PROGRAM
JP7710755B2 (ja) * 2021-08-18 2025-07-22 国立大学法人東北大学 子宮内膜モデル、子宮内膜モデルの作製方法、及び子宮内膜着床モデル
EP4446408A4 (en) * 2021-12-06 2025-04-02 Ricoh Company, Ltd. Hydrogel, inkjet ink, method for producing cell culture body, and cell-containing gel particles and method for producing same

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003070466A (ja) 2001-09-05 2003-03-11 Shiseido Co Ltd 人工毛球部及びその製造方法、並びに、人工毛球部を用いた薬剤の評価方法
WO2006129672A1 (ja) 2005-05-30 2006-12-07 Tokyo University Of Science Educational Foundation Administrative Organization 歯の製造方法、歯の集合体及び組織の製造方法
JP2008029331A (ja) 2006-06-27 2008-02-14 Shiseido Co Ltd 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊
WO2012108069A1 (ja) 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ ガイドを有する移植用再生器官原基の製造方法、当該方法によって製造される、ガイドを有する移植用再生器官原基を含む組成物、およびガイドを有する移植用再生器官原基の移植方法
JP2016522682A (ja) 2013-05-03 2016-08-04 ザ ヘンリー エム． ジャクソン ファウンデーション フォー ザ アドヴァンスメント オブ ミリタリー メディシン インコーポレイテッド 代用皮膚および毛包新生の方法
JP2017051160A (ja) 2015-09-11 2017-03-16 国立大学法人横浜国立大学 細胞包埋ビーズ及びその製造方法
WO2017073625A1 (ja) 2015-10-30 2017-05-04 国立大学法人横浜国立大学 再生毛包原基の集合体の製造方法、毛包組織含有シート、及び毛包組織含有シートの製造方法
WO2017217393A1 (ja) 2016-06-17 2017-12-21 国立大学法人横浜国立大学 毛髪再生用細胞包埋ビーズ及びその製造方法、並びに毛髪再生用キット

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995018216A1 (en) * 1993-12-30 1995-07-06 Nitta Gelatin Inc. Process for embedding culture of animal cells
CN101479376A (zh) 2006-06-27 2009-07-08 株式会社资生堂 含有源自身体的多个细胞种类的、能形成原始的器官样的细胞块
WO2008112170A1 (en) * 2007-03-09 2008-09-18 Corning Incorporated Three dimensional gum matrices for cell culture, manufacturing methods and methods of use
JP6000021B2 (ja) 2012-08-23 2016-09-28 国立大学法人東北大学 Ｉｇｆを含む成長因子による歯、再生歯および再生歯胚における歯冠および咬頭ならびに歯根の大きさおよび形の制御の方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003070466A (ja) 2001-09-05 2003-03-11 Shiseido Co Ltd 人工毛球部及びその製造方法、並びに、人工毛球部を用いた薬剤の評価方法
WO2006129672A1 (ja) 2005-05-30 2006-12-07 Tokyo University Of Science Educational Foundation Administrative Organization 歯の製造方法、歯の集合体及び組織の製造方法
JP2008029331A (ja) 2006-06-27 2008-02-14 Shiseido Co Ltd 体性に由来する複数の細胞種からなる原始的な器官様をなし得る細胞塊
WO2012108069A1 (ja) 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ ガイドを有する移植用再生器官原基の製造方法、当該方法によって製造される、ガイドを有する移植用再生器官原基を含む組成物、およびガイドを有する移植用再生器官原基の移植方法
JP2016522682A (ja) 2013-05-03 2016-08-04 ザ ヘンリー エム． ジャクソン ファウンデーション フォー ザ アドヴァンスメント オブ ミリタリー メディシン インコーポレイテッド 代用皮膚および毛包新生の方法
JP2017051160A (ja) 2015-09-11 2017-03-16 国立大学法人横浜国立大学 細胞包埋ビーズ及びその製造方法
WO2017073625A1 (ja) 2015-10-30 2017-05-04 国立大学法人横浜国立大学 再生毛包原基の集合体の製造方法、毛包組織含有シート、及び毛包組織含有シートの製造方法
WO2017217393A1 (ja) 2016-06-17 2017-12-21 国立大学法人横浜国立大学 毛髪再生用細胞包埋ビーズ及びその製造方法、並びに毛髪再生用キット

Also Published As

Publication number Publication date
US12312581B2 (en) 2025-05-27
EP3795683A4 (en) 2022-04-06
JP2019198267A (ja) 2019-11-21
US20210163918A1 (en) 2021-06-03
WO2019220843A1 (ja) 2019-11-21
EP3795683A1 (en) 2021-03-24

Similar Documents

Publication Publication Date Title
KR101840078B1 (ko) 가이드를 갖는 이식용 재생 기관 원기의 제조방법, 해당 방법에 의해 제조되는, 가이드를 갖는 이식용 재생 기관 원기를 포함하는 조성물 및 가이드를 갖는 이식용 재생 기관 원기의 이식방법
JP5887333B2 (ja) 毛色が制御された移植用再生毛包原基の製造方法、当該移植用再生毛包原基を含む組成物、およびその移植方法
Ajmal et al. Organ regeneration through stem cells and tissue engineering
JP7142840B2 (ja) 細胞含有ハイドロゲル体及びその製造方法
JP6475322B2 (ja) ３次元細胞培養システム及びこれを用いた細胞培養方法
JP6999132B2 (ja) 再生毛包原基を有する培養皮膚の製造方法及びその使用
JP2018082638A (ja) 毛細血管構造を有する再生毛包原基の集合体の製造方法、毛包組織含有シート、及び毛包組織含有シートの製造方法
WO2023058429A1 (ja) 毛髪再生能を有する細胞凝集塊の製造方法及びこれに関連する方法
JP7427180B2 (ja) 毛包原基及びその製造方法
JP7403734B2 (ja) ファイバー担体－細胞含有ゲル複合体及びその製造方法、並びにファイバー担体－細胞含有ゲル複合体製造用キット
JP7444367B2 (ja) 増幅毛包間葉系細胞の製造方法及びその使用
JP7246595B2 (ja) 毛包原基、毛包原基の製造方法、及び毛包原基に含まれる細胞の活性化方法
Barhouse et al. Home away from home: bioengineering advancements to mimic the developmental and adult stem cell niche
WO2022070787A1 (ja) 組織体の製造方法及び組織体を用いた疾患の治療方法
JP7303962B2 (ja) 培養容器、及び細胞－担体複合体の製造方法
WO2021065665A1 (ja) 三次元細胞構造体の製造方法
JP5249789B2 (ja) 毛髪の形成方法及び生物材料
Buttery et al. A brief introduction to different cell types
JP2023056592A (ja) 移植用毛様組織及びこれに関連する方法
JP2024105540A (ja) 毛包上皮性幹細胞の生体外増殖方法
Semenov Engineering mesenchymal stem cell sheets on functional polyelectrolyte multilayer substrates
Semenov ETH Library

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210312

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210312

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220329

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220512

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220823

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220902

R150 Certificate of patent or registration of utility model

Ref document number: 7142840

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250