JP7123168B2 - 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法 - Google Patents

動物細胞、動物細胞の製造方法および目的タンパク質の製造方法 Download PDF

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JP7123168B2
JP7123168B2 JP2020559242A JP2020559242A JP7123168B2 JP 7123168 B2 JP7123168 B2 JP 7123168B2 JP 2020559242 A JP2020559242 A JP 2020559242A JP 2020559242 A JP2020559242 A JP 2020559242A JP 7123168 B2 JP7123168 B2 JP 7123168B2
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cells
culture
snat2
gene encoding
cell
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JPWO2020122049A1 (ja
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裕也 渡辺
達也 松浦
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Fujifilm Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
  • Molecular Biology (AREA)
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  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020559242A 2018-12-11 2019-12-10 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法 Active JP7123168B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018231592 2018-12-11
JP2018231592 2018-12-11
PCT/JP2019/048216 WO2020122049A1 (ja) 2018-12-11 2019-12-10 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法

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JPWO2020122049A1 JPWO2020122049A1 (ja) 2021-10-28
JP7123168B2 true JP7123168B2 (ja) 2022-08-22

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US (1) US20210301262A1 (de)
EP (1) EP3896168A4 (de)
JP (1) JP7123168B2 (de)
CN (1) CN113242907A (de)
WO (1) WO2020122049A1 (de)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006230252A (ja) 2005-02-23 2006-09-07 Nara Institute Of Science & Technology ポリペプチド発現システム
WO2009054433A1 (ja) 2007-10-24 2009-04-30 Chugai Seiyaku Kabushiki Kaisha 異種タンパク質製造のための細胞及びそれを用いた製造方法
JP2018509904A (ja) 2015-04-03 2018-04-12 セレクシス エス．エー． 哺乳動物細胞における組換えタンパク質生産のためのビタミンならびにビタミン代謝関連の遺伝子およびタンパク質の使用
JP2018527009A (ja) 2015-09-23 2018-09-20 ファイザー・インク 細胞および細胞培養の方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5337033B2 (ja) 2007-08-07 2013-11-06 中外製薬株式会社 異種タンパク質の製造方法
CN102321656A (zh) * 2011-07-06 2012-01-18 上海师范大学 谷氨酰胺转运蛋白2-加强型绿色荧光蛋白融合蛋白表达载体及其构建方法和应用
BR112015027373A2 (pt) 2013-05-03 2017-08-29 Fujifilm Diosynth Biotechnologies Uk Ltd Processo de expressão

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006230252A (ja) 2005-02-23 2006-09-07 Nara Institute Of Science & Technology ポリペプチド発現システム
WO2009054433A1 (ja) 2007-10-24 2009-04-30 Chugai Seiyaku Kabushiki Kaisha 異種タンパク質製造のための細胞及びそれを用いた製造方法
JP2018509904A (ja) 2015-04-03 2018-04-12 セレクシス エス．エー． 哺乳動物細胞における組換えタンパク質生産のためのビタミンならびにビタミン代謝関連の遺伝子およびタンパク質の使用
JP2018527009A (ja) 2015-09-23 2018-09-20 ファイザー・インク 細胞および細胞培養の方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CLONTECH Laboratories Inc.，pEGFP-C2 Vector Information，1997年，http://www.yrgene.com/documents/vector/pegfp-c2mapmcs.pdf
DatabaseDDBJ/EMBL/GenBank [online]，[retrieved on 2022-05-06]，2001年04月23日，Accession No.AF259799，https://www.ncbi.nlm.nih.gov/nuccore/AF259799
HATANAKA, T., et al.，Amino acid transporter ATA2 is stored at the trans-Golgi network and released by insulin stimulus in，The Journal of Biological Chemistry，2006年，Vol.281, No.51，pp.39273-39284

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Publication number Publication date
EP3896168A1 (de) 2021-10-20
US20210301262A1 (en) 2021-09-30
JPWO2020122049A1 (ja) 2021-10-28
CN113242907A (zh) 2021-08-10
WO2020122049A1 (ja) 2020-06-18
EP3896168A4 (de) 2022-03-02

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