JP7098270B2 - 改善された凝乳特性を有するキモシンの変異体 - Google Patents

改善された凝乳特性を有するキモシンの変異体 Download PDF

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JP7098270B2
JP7098270B2 JP2016553838A JP2016553838A JP7098270B2 JP 7098270 B2 JP7098270 B2 JP 7098270B2 JP 2016553838 A JP2016553838 A JP 2016553838A JP 2016553838 A JP2016553838 A JP 2016553838A JP 7098270 B2 JP7098270 B2 JP 7098270B2
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polypeptide
chymosin
seq
amino acid
variant
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JP2017512059A (ja
Inventor
マルデン ファン デン ブレンク ヨハネス
ラングホルム イェンスン イェスパ
ヤコプスン ヨーナス
ロン マーティン
イェベスン イーベン
イェーッケル クレスチャン
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セーホーエル．ハンセン アクティーゼルスカブ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/04—Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/04—Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
    • A23C19/041—Proteolytic or milk clotting enzymes from plants or vegetables, e.g. papain, ficin, bromelain
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1209—Proteolytic or milk coagulating enzymes, e.g. trypsine
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Husbandry (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016553838A 2014-02-26 2015-02-26 改善された凝乳特性を有するキモシンの変異体 Active JP7098270B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14156707 2014-02-26
EP14156707.3 2014-02-26
EP14176664.2 2014-07-11
EP14176664 2014-07-11
PCT/EP2015/054020 WO2015128417A1 (en) 2014-02-26 2015-02-26 Variants of chymosin with improved milk-clotting properties

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020038155A Division JP7144470B2 (ja) 2014-02-26 2020-03-05 改善された凝乳特性を有するキモシンの変異体

Publications (2)

Publication Number Publication Date
JP2017512059A JP2017512059A (ja) 2017-05-18
JP7098270B2 true JP7098270B2 (ja) 2022-07-11

Family

ID=52596486

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2016553838A Active JP7098270B2 (ja) 2014-02-26 2015-02-26 改善された凝乳特性を有するキモシンの変異体
JP2020038155A Active JP7144470B2 (ja) 2014-02-26 2020-03-05 改善された凝乳特性を有するキモシンの変異体
JP2022085229A Pending JP2022122926A (ja) 2014-02-26 2022-05-25 改善された凝乳特性を有するキモシンの変異体

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2020038155A Active JP7144470B2 (ja) 2014-02-26 2020-03-05 改善された凝乳特性を有するキモシンの変異体
JP2022085229A Pending JP2022122926A (ja) 2014-02-26 2022-05-25 改善された凝乳特性を有するキモシンの変異体

Country Status (14)

Country Link
US (4) US10982204B2 (https=)
EP (2) EP3110948B1 (https=)
JP (3) JP7098270B2 (https=)
KR (2) KR102494645B1 (https=)
CN (2) CN106062187B (https=)
AU (2) AU2015222149B2 (https=)
BR (1) BR112016017502B1 (https=)
CA (1) CA2938015A1 (https=)
DK (1) DK3110948T3 (https=)
MX (2) MX2016009937A (https=)
NZ (1) NZ722003A (https=)
RU (2) RU2717770C2 (https=)
UA (1) UA121380C2 (https=)
WO (1) WO2015128417A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ702030A (en) 2012-05-25 2016-11-25 Chr Hansen As Variants of chymosin with improved milk-clotting properties
RU2717770C2 (ru) 2014-02-26 2020-03-25 Кхр. Хансен А/С Варианты химозина с улучшенными молокосвертывающими свойствами
RU2740317C2 (ru) * 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
EP3344049A1 (en) * 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
ES3023033T3 (en) * 2015-09-18 2025-05-29 Dsm Ip Assets Bv Yogurt with reduced ripening
EP3457859A1 (en) * 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10961524B2 (en) 2016-05-19 2021-03-30 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
CN106754840B (zh) * 2016-12-22 2019-06-21 江南大学 一种酶活及热稳定性改善的凝乳酶突变体
US20200345022A1 (en) 2018-01-15 2020-11-05 Chr. Hansen A/S Fermented milk product and preparation thereof using phospholipase
CN113801867B (zh) * 2021-11-18 2022-03-18 中国农业科学院生物技术研究所 骆驼凝乳酶原突变蛋白及其编码基因和应用
US20260000087A1 (en) 2022-04-06 2026-01-01 Chr. Hansen A/S Aspartic Protease, Methods and Uses Thereof
WO2024189187A1 (en) 2023-03-15 2024-09-19 Dsm Ip Assets B.V. Novel process for producing a fermented milk product and new fermented milk product so produced

Citations (2)

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WO2013164481A1 (en) 2012-05-03 2013-11-07 Dsm Ip Assets B.V. Improved enzyme variants
WO2013174840A1 (en) 2012-05-25 2013-11-28 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

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EP0123928A3 (en) 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US5217891A (en) * 1987-07-28 1993-06-08 Chiron Corporation DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides
US7390936B1 (en) * 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
DK1334182T4 (da) * 2000-11-06 2021-04-26 Eidgenoessische Technische Hochschule Eth Fremgangsmåde til fremstilling af ikke-bovint chymosin og anvendelse heraf
AU2003282724B2 (en) 2002-10-02 2010-03-04 Catalyst Biosciences, Inc. Methods of generating and screening for proteases with altered specificity
WO2004072225A2 (en) 2003-02-12 2004-08-26 Ramot At Tel Aviv University Ltd. Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi
BRPI0412007A (pt) 2003-06-27 2006-08-15 Bioren Inc mutagênese look-through
EP1745068A4 (en) * 2004-03-30 2009-11-04 Sudershan Biotech Ltd RECOMBINANT CALM CHYMOSIN AND PROCESS FOR PRODUCING THE SAME
EP3763220A1 (en) 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
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DK2602317T3 (da) * 2009-11-20 2017-11-13 Danisco Us Inc Beta-glucosidasevarianter med forbedrede egenskaber
KR20130102534A (ko) * 2010-07-12 2013-09-17 에이티와이알 파마, 인코포레이티드 히스티딜­trna 합성효소의 단백질 단편에 관련된 치료적, 진단적, 및 항체 조성물의 혁신적 발견
CN103484488B (zh) * 2013-09-16 2015-07-15 中国农业科学院生物技术研究所 优化的牛凝乳酶原基因及其分泌表达方法和应用
RU2717770C2 (ru) 2014-02-26 2020-03-25 Кхр. Хансен А/С Варианты химозина с улучшенными молокосвертывающими свойствами
US20180110234A1 (en) 2015-02-10 2018-04-26 Chr. Hansen A/S Blends of chymosins with improved milk-clotting properties
RU2740317C2 (ru) 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
EP3344049A1 (en) 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
EP3457859A1 (en) 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10961524B2 (en) 2016-05-19 2021-03-30 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013164481A1 (en) 2012-05-03 2013-11-07 Dsm Ip Assets B.V. Improved enzyme variants
WO2013164479A2 (en) 2012-05-03 2013-11-07 Dsm Ip Assets B.V. Improved enzyme variants
WO2013174840A1 (en) 2012-05-25 2013-11-28 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

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Publication number Publication date
AU2015222149B2 (en) 2021-09-23
US20220154162A1 (en) 2022-05-19
KR20160125459A (ko) 2016-10-31
DK3110948T3 (da) 2025-05-12
KR102344861B1 (ko) 2021-12-29
JP2020141667A (ja) 2020-09-10
AU2021286442A1 (en) 2022-01-20
CA2938015A1 (en) 2015-09-03
BR112016017502A2 (https=) 2017-08-08
BR112016017502B1 (pt) 2023-10-24
US20170067041A1 (en) 2017-03-09
AU2015222149A1 (en) 2016-07-28
WO2015128417A1 (en) 2015-09-03
NZ722003A (en) 2023-06-30
JP2017512059A (ja) 2017-05-18
UA121380C2 (uk) 2020-05-25
CN106062187A (zh) 2016-10-26
US11827913B2 (en) 2023-11-28
MX2016009937A (es) 2016-10-31
JP7144470B2 (ja) 2022-09-29
EP4627930A2 (en) 2025-10-08
KR20220002714A (ko) 2022-01-06
JP2022122926A (ja) 2022-08-23
EP3110948B1 (en) 2025-04-09
RU2016132974A (ru) 2018-03-27
CN106062187B (zh) 2020-09-15
CN111944791A (zh) 2020-11-17
KR102494645B1 (ko) 2023-02-06
RU2717770C2 (ru) 2020-03-25
RU2739004C1 (ru) 2020-12-21
US20210230571A1 (en) 2021-07-29
US11629343B2 (en) 2023-04-18
US20240084281A1 (en) 2024-03-14
EP4627930A3 (en) 2026-03-04
AU2021286442B2 (en) 2024-12-12
RU2016132974A3 (https=) 2018-09-18
US10982204B2 (en) 2021-04-20
MX2022006692A (es) 2022-07-11
EP3110948A1 (en) 2017-01-04

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