CN106062187B - 具有提高的凝乳特性的凝乳酶变体 - Google Patents

具有提高的凝乳特性的凝乳酶变体 Download PDF

Info

Publication number
CN106062187B
CN106062187B CN201580009539.6A CN201580009539A CN106062187B CN 106062187 B CN106062187 B CN 106062187B CN 201580009539 A CN201580009539 A CN 201580009539A CN 106062187 B CN106062187 B CN 106062187B
Authority
CN
China
Prior art keywords
polypeptide
chymosin
seq
variant
rennet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580009539.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN106062187A (zh
Inventor
约翰尼斯·马腾·范丹尼布林克
J·L·詹森
J·雅各布森
马丁·伦德
伊本·杰普森
C·杰克尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Section Hansen Co ltd
Original Assignee
Section Hansen Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Section Hansen Co ltd filed Critical Section Hansen Co ltd
Priority to CN202010904707.0A priority Critical patent/CN111944791A/zh
Publication of CN106062187A publication Critical patent/CN106062187A/zh
Application granted granted Critical
Publication of CN106062187B publication Critical patent/CN106062187B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/04—Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/04—Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
    • A23C19/041—Proteolytic or milk clotting enzymes from plants or vegetables, e.g. papain, ficin, bromelain
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1209—Proteolytic or milk coagulating enzymes, e.g. trypsine
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Husbandry (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580009539.6A 2014-02-26 2015-02-26 具有提高的凝乳特性的凝乳酶变体 Active CN106062187B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010904707.0A CN111944791A (zh) 2014-02-26 2015-02-26 具有提高的凝乳特性的凝乳酶变体

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14156707 2014-02-26
EP14156707.3 2014-02-26
EP14176664.2 2014-07-11
EP14176664 2014-07-11
PCT/EP2015/054020 WO2015128417A1 (en) 2014-02-26 2015-02-26 Variants of chymosin with improved milk-clotting properties

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202010904707.0A Division CN111944791A (zh) 2014-02-26 2015-02-26 具有提高的凝乳特性的凝乳酶变体

Publications (2)

Publication Number Publication Date
CN106062187A CN106062187A (zh) 2016-10-26
CN106062187B true CN106062187B (zh) 2020-09-15

Family

ID=52596486

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201580009539.6A Active CN106062187B (zh) 2014-02-26 2015-02-26 具有提高的凝乳特性的凝乳酶变体
CN202010904707.0A Pending CN111944791A (zh) 2014-02-26 2015-02-26 具有提高的凝乳特性的凝乳酶变体

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202010904707.0A Pending CN111944791A (zh) 2014-02-26 2015-02-26 具有提高的凝乳特性的凝乳酶变体

Country Status (14)

Country Link
US (4) US10982204B2 (https=)
EP (2) EP3110948B1 (https=)
JP (3) JP7098270B2 (https=)
KR (2) KR102494645B1 (https=)
CN (2) CN106062187B (https=)
AU (2) AU2015222149B2 (https=)
BR (1) BR112016017502B1 (https=)
CA (1) CA2938015A1 (https=)
DK (1) DK3110948T3 (https=)
MX (2) MX2016009937A (https=)
NZ (1) NZ722003A (https=)
RU (2) RU2717770C2 (https=)
UA (1) UA121380C2 (https=)
WO (1) WO2015128417A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ702030A (en) 2012-05-25 2016-11-25 Chr Hansen As Variants of chymosin with improved milk-clotting properties
RU2717770C2 (ru) 2014-02-26 2020-03-25 Кхр. Хансен А/С Варианты химозина с улучшенными молокосвертывающими свойствами
RU2740317C2 (ru) * 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
EP3344049A1 (en) * 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
ES3023033T3 (en) * 2015-09-18 2025-05-29 Dsm Ip Assets Bv Yogurt with reduced ripening
EP3457859A1 (en) * 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10961524B2 (en) 2016-05-19 2021-03-30 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
CN106754840B (zh) * 2016-12-22 2019-06-21 江南大学 一种酶活及热稳定性改善的凝乳酶突变体
US20200345022A1 (en) 2018-01-15 2020-11-05 Chr. Hansen A/S Fermented milk product and preparation thereof using phospholipase
CN113801867B (zh) * 2021-11-18 2022-03-18 中国农业科学院生物技术研究所 骆驼凝乳酶原突变蛋白及其编码基因和应用
US20260000087A1 (en) 2022-04-06 2026-01-01 Chr. Hansen A/S Aspartic Protease, Methods and Uses Thereof
WO2024189187A1 (en) 2023-03-15 2024-09-19 Dsm Ip Assets B.V. Novel process for producing a fermented milk product and new fermented milk product so produced

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013164481A1 (en) * 2012-05-03 2013-11-07 Dsm Ip Assets B.V. Improved enzyme variants
WO2013174840A1 (en) * 2012-05-25 2013-11-28 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123928A3 (en) 1983-03-31 1986-02-05 Codon Genetic Engineering Laboratories Recombinant dna coding for a polypeptide displaying milk clotting activity
US5217891A (en) * 1987-07-28 1993-06-08 Chiron Corporation DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides
US7390936B1 (en) * 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
DK1334182T4 (da) * 2000-11-06 2021-04-26 Eidgenoessische Technische Hochschule Eth Fremgangsmåde til fremstilling af ikke-bovint chymosin og anvendelse heraf
AU2003282724B2 (en) 2002-10-02 2010-03-04 Catalyst Biosciences, Inc. Methods of generating and screening for proteases with altered specificity
WO2004072225A2 (en) 2003-02-12 2004-08-26 Ramot At Tel Aviv University Ltd. Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi
BRPI0412007A (pt) 2003-06-27 2006-08-15 Bioren Inc mutagênese look-through
EP1745068A4 (en) * 2004-03-30 2009-11-04 Sudershan Biotech Ltd RECOMBINANT CALM CHYMOSIN AND PROCESS FOR PRODUCING THE SAME
EP3763220A1 (en) 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
EP2411512B1 (en) * 2009-03-24 2013-11-20 Meito Sangyo Co., Ltd. Improved type milk-clotting protease derived from a microorganism
DK2602317T3 (da) * 2009-11-20 2017-11-13 Danisco Us Inc Beta-glucosidasevarianter med forbedrede egenskaber
KR20130102534A (ko) * 2010-07-12 2013-09-17 에이티와이알 파마, 인코포레이티드 히스티딜­trna 합성효소의 단백질 단편에 관련된 치료적, 진단적, 및 항체 조성물의 혁신적 발견
CN103484488B (zh) * 2013-09-16 2015-07-15 中国农业科学院生物技术研究所 优化的牛凝乳酶原基因及其分泌表达方法和应用
RU2717770C2 (ru) 2014-02-26 2020-03-25 Кхр. Хансен А/С Варианты химозина с улучшенными молокосвертывающими свойствами
US20180110234A1 (en) 2015-02-10 2018-04-26 Chr. Hansen A/S Blends of chymosins with improved milk-clotting properties
RU2740317C2 (ru) 2015-06-22 2021-01-13 Кхр. Хансен А/С Варианты химозина с улучшенными свойствами
EP3344049A1 (en) 2015-08-31 2018-07-11 Chr. Hansen A/S Variants of chymosin with improved properties
EP3457859A1 (en) 2016-05-19 2019-03-27 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
US10961524B2 (en) 2016-05-19 2021-03-30 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013164481A1 (en) * 2012-05-03 2013-11-07 Dsm Ip Assets B.V. Improved enzyme variants
WO2013174840A1 (en) * 2012-05-25 2013-11-28 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
凝乳酶β-转角突变体的构建、表达和性质分析;黎红晔;《生物工程学报》;19980131;39-45 *

Also Published As

Publication number Publication date
AU2015222149B2 (en) 2021-09-23
US20220154162A1 (en) 2022-05-19
KR20160125459A (ko) 2016-10-31
DK3110948T3 (da) 2025-05-12
KR102344861B1 (ko) 2021-12-29
JP2020141667A (ja) 2020-09-10
AU2021286442A1 (en) 2022-01-20
CA2938015A1 (en) 2015-09-03
BR112016017502A2 (https=) 2017-08-08
BR112016017502B1 (pt) 2023-10-24
US20170067041A1 (en) 2017-03-09
AU2015222149A1 (en) 2016-07-28
WO2015128417A1 (en) 2015-09-03
NZ722003A (en) 2023-06-30
JP7098270B2 (ja) 2022-07-11
JP2017512059A (ja) 2017-05-18
UA121380C2 (uk) 2020-05-25
CN106062187A (zh) 2016-10-26
US11827913B2 (en) 2023-11-28
MX2016009937A (es) 2016-10-31
JP7144470B2 (ja) 2022-09-29
EP4627930A2 (en) 2025-10-08
KR20220002714A (ko) 2022-01-06
JP2022122926A (ja) 2022-08-23
EP3110948B1 (en) 2025-04-09
RU2016132974A (ru) 2018-03-27
CN111944791A (zh) 2020-11-17
KR102494645B1 (ko) 2023-02-06
RU2717770C2 (ru) 2020-03-25
RU2739004C1 (ru) 2020-12-21
US20210230571A1 (en) 2021-07-29
US11629343B2 (en) 2023-04-18
US20240084281A1 (en) 2024-03-14
EP4627930A3 (en) 2026-03-04
AU2021286442B2 (en) 2024-12-12
RU2016132974A3 (https=) 2018-09-18
US10982204B2 (en) 2021-04-20
MX2022006692A (es) 2022-07-11
EP3110948A1 (en) 2017-01-04

Similar Documents

Publication Publication Date Title
CN106062187B (zh) 具有提高的凝乳特性的凝乳酶变体
AU2022200663B2 (en) Variants of chymosin with improved milk-clotting properties

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant