JP7071725B2 - Antiviral detergent composition - Google Patents

Description

The present invention relates to an anti-virus cleaning agent composition exhibiting an inactivating effect on a virus, an anti-virus cleaning material containing the same, and a method for inactivating a virus using these.

In recent years, the number of cases of food poisoning and the number of patients have the highest number of cases in which norovirus is the causative agent, which has led to the outbreak of large-scale food poisoning due to its strong infectivity, which has become a problem. It has been said that the cause of food poisoning caused by norovirus is to eat oysters and other bivalves, but recently, norovirus contained in food, feces and vomitus is transmitted from person to person, and further. It has become clear that secondary contamination is the main cause, such as spreading by contact with clean surfaces through contaminated food and water.

In order to prevent this secondary contamination, it is necessary to thoroughly wash your hands, as well as food contaminated with norovirus, feces and vomitus, and cooking utensils and utensils to which they adhere, cooking tables and work tables, toilet bowls and toilet seats, and water supply. It is important to effectively and safely clean the surface of toilets, door knobs at doorways, floors and walls.

"Norovirus" belongs to the genus Norovirus of the Caliciviridae family, and is an RNA-type virus that has only RNA. It has a structure in which RNA is covered with a protein shell called capsid, and a membrane called envelope, which consists of sugar and lipid, is do not have.

In general, a virus having an envelope can be inactivated because the envelope is easily destroyed by the drug and cannot bind to the receptor of the host cell. However, norovirus does not have this envelope and is therefore resistant to the drug.

As a method for inactivating norovirus, a method using a sodium hypochlorite solution is known (Non-Patent Document 1). However, in the cleaning method using a sodium hypochlorite solution, if the object to be cleaned is a hard metal surface or a hard plastic surface, rust, deterioration, corrosion and the like may occur. Further, depending on the object to be cleaned, there is a problem that the color is decolorized due to the bleaching action / effect of sodium hypochlorite. In addition, there is a problem that the odor of sodium hypochlorite spreads in the facility to be cleaned, and if it is accidentally used in combination with an acidic detergent, highly toxic chlorine gas will be generated. In the worst case, it may lead to death.

Further, although many existing detergent compositions containing sodium hypochlorite as an active ingredient (sodium hypochlorite-based detergent compositions) have an inactivating effect on norovirus, the object to be cleaned is feces. It has been reported that the effect is reduced or eliminated when it is contaminated with vomit or vomit, that is, in the presence of organic stains (Non-Patent Document 1). In the virus inactivation test described in Non-Patent Document 1, feline calicivirus, which is a substitute virus for norovirus, is used. Feline calicivirus F9 strain is commonly used as an alternative virus in the United States Environmental Protection Agency (EPA) norovirus test method because norovirus has not been successfully transmitted to cultured cells and animal experiments. In the case of tests and experiments, feline calicivirus is currently used as a substitute virus for norovirus in Japan and overseas.

Further, in Japanese Patent No. 4647912 (Patent Document 1), (a) a quaternary ammonium salt represented by a predetermined general formula; and (b) monoethanolamine in a mass ratio of (a): (b). Disinfectant compositions containing 20: 1 to 1:20 have been disclosed for use as surface disinfectants except for human skin surfaces, equipment disinfection or laundry disinfection, or as antiviral agents in chemical patents. There is. However, the virus-killing agent is said to be against parvovirus, picornavirus or poliovirus, and its effectiveness against norovirus and its effectiveness in the presence of organic stains are not clear.

Japanese Patent No. 4647912

"2015 Survey Report on Norovirus Inactivation Conditions" National Institute of Health Sciences Food Hygiene Management Department, November 18, 2016

The present invention has been made in view of such circumstances, and an object of the present invention is to provide a non-sodium hypochlorite-based antiviral detergent composition having an inactivating effect on norovirus.

As a result of diligent studies to solve the above problems, the present inventor has a pH value of 11 or more for a detergent composition containing a quaternary ammonium salt as an active ingredient (quaternary ammonium salt-based detergent composition). It has been found that an inactivating effect on norovirus can be obtained when it is within the range.

Further, it has been found that the inactivating effect of the quaternary ammonium salt-based detergent composition on norovirus is reduced or eliminated when the pH value fluctuates to less than 11 due to organic contamination. Therefore, by setting the pH value of the detergent composition to be more alkaline, the detergent composition can maintain a pH of 11 or higher even in the presence of organic stains, and the inactivating effect on norovirus is reduced. Or it was found that it did not disappear.

The present invention is based on these findings and includes the following inventions.
[1] The following components (A) to (C) are contained in the following ratio with respect to the entire composition, water is contained as the component (D), and the pH of the composition (JIS Z-8802: 2011 ". pH measuring method ") is 11 or more at 25 ° C., an anti-virus detergent composition:
(A) Quaternary ammonium salt 0.5-5% by mass
(B) Nonionic surfactant and / or amphoteric surfactant 1 to 20% by mass,
(C) Alkaline agent 0.07 to 10.0% by mass.
[2] The antiviral cleaning agent composition according to [1], wherein the quaternary ammonium salt is benzalkonium chloride, benzethonium chloride or didecyldimethylammonium chloride.
[3] The nonionic surfactant and / or amphoteric surfactant is at least one selected from polyoxyalkylene alkyl ethers, alkyl polyglucosides, amine oxides, and fatty acid alkanolamides [1]. Or the antiviral detergent composition of [2].
[4] The antiviral detergent composition according to any one of [1] to [3], wherein the alkaline agent is at least one selected from inorganic alkaline substances.
[5] The antiviral detergent composition according to any one of [1] to [4], which further contains a chelating agent and / or a water-soluble solvent.

[6] The antiviral cleaning agent composition according to [5], wherein the chelating agent is at least one selected from hydroxycarboxylic acid and a salt thereof, and aminocarboxylic acid and a salt thereof.
[7] The antiviral detergent composition according to [5], wherein the water-soluble solvent is at least one selected from a glycol-based solvent and an alcohol.
[8] The antiviral detergent composition according to any one of [1] to [7], wherein the pH (JIS Z-8802: 2011 "pH measuring method") exceeds 12.1 at 25 ° C.
[9] An antiviral cleaning material, which comprises impregnating a non-woven fabric with the antiviral cleaning agent composition according to any one of [1] to [8].
[10] A method for using an antiviral cleaning material, which comprises using the antiviral cleaning material according to [9] for inactivating a virus.

[11] A method for inactivating a virus, which comprises spraying the antiviral cleaning agent composition according to any one of [1] to [8] onto the surface of an object to be cleaned.

According to the present invention, it is possible to provide a quaternary ammonium salt-based antiviral detergent composition having an inactivating effect on viruses, particularly norovirus.

The anti-virus detergent composition of the present invention (hereinafter, may be simply referred to as "the composition of the present invention") comprises (A) a quaternary ammonium salt, (B) a nonionic surfactant and / or It is obtained by using an amphoteric surfactant, (C) an alkaline agent, and (D) water.

As the quaternary ammonium salt (component (A)) used in the composition of the present invention, those generally used for antibacterial cleaning agents can be used, for example, benzalkonium chloride, benzethonium chloride, chloride. Didecyldimethylammonium, tetramethylammonium chloride, tetrabutylammonium chloride, methylbenzethonium chloride, disstearyldimethylammonium chloride, cetylpyridinium chloride, alkyltrimethylammonium chloride, octyltrimethylammonium chloride, decyltrimethylammonium chloride, dodecyldimethylbenzylammonium chloride, Dodecyltrimethylammonium chloride, tetradecyltrimethylammonium chloride, hexadecyltrimethylammonium chloride, cetyltrimethylammonium chloride, stearyltrimethylammonium chloride, benzyltrimethylammonium chloride, benzyltriethylammonium chloride, dialkyldimethylammonium chloride, tetramethylammonium hydroxide, bromide Examples thereof include, but are not limited to, benzalconium, cetrimonium bromide, domiphen bromide, alkyltrimethylammonium bromide, and hexadecyltrimethylammonium bromide. Preferably, benzalkonium chloride, benzethonium chloride, and didecyldimethylammonium chloride can be utilized. In the composition of the present invention, the quaternary ammonium salt selected from the above may be used alone or in combination of two or more. For example, benzalkonium chloride can be preferably used in the composition of the present invention.

In the composition of the present invention, the quaternary ammonium salt is set in the range of 0.5 to 5% by mass, preferably 0.5 to 2.5% by mass, and more preferably 1 to 2% by mass. Can be included in quantity. If the amount of the quaternary ammonium salt is small, the inactivating effect of the virus may not be obtained or the effect may be poor, while if the amount of the quaternary ammonium salt is too large, the inactivating effect of the virus may not be obtained. May be saturated and rather economically disadvantageous.

As the nonionic surfactant and / or amphoteric surfactant (component (B)) used in the composition of the present invention, those generally used for antibacterial detergents can be used, for example, polyoxy. Examples thereof include nonionic surfactants such as alkylene alkyl ethers, alkyl polyglucosides and fatty acid alkanolamides, and amphoteric surfactants such as amine oxides.

Examples of the polyoxyalkylene alkyl ether include polyoxyethylene alkyl ether, polyoxypropylene alkyl ether, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene alkyl phenyl ether, polyoxypropylene alkyl phenyl ether, and polyoxyethylene polyoxy. Examples thereof include propylene alkyl phenyl ether, but the present invention is not limited thereto. A primary or secondary alcohol preferably having an average carbon number of 8 to 18, preferably 9 to 16, more preferably 9 to 15, with an average of 2 to 20 mol, preferably 2 to 15 mol. Ethylene oxide or propylene oxide added to the above can be used.

As an alkyl polyglucoside, the following formula:
R ^1- (OR ² ) _m (R ³ ) _n
[In the formula, R ¹ represents a linear or branched alkyl group, alkenyl group or alkylphenyl group having an average carbon number of 8 to 18 carbon atoms.
R ² represents an alkylene group having 2 to 4 carbon atoms.
R ³ indicates a residue derived from a reducing sugar having 5 to 6 carbon atoms.
m indicates an integer from 0 to 5 and represents
n indicates an integer such as 1 to 2. ]
1, but preferably, R ¹ is an alkyl group having an average carbon number of 8 to 16, R ² is an alkylene group having 2 to 4 carbon atoms, and Z is glucose, fructose, and the like. Those that are residues derived from maltose or sucrose can be used.

Examples of the fatty acid alkanolamide include those produced by reacting an alkanolamine with a fatty acid, a fatty acid alkyl ester, a fatty acid chloride and the like, and are not particularly limited.

As an amine oxide, the following formula:
R ₃ N ^{+ -O-}
[In the formula, R represents hydrogen, alkyl, aryl, arylalkyl, heteroalkyl, heteroaryl, heteroarylalkyl, cycloalkyl, cycloheteroalkyl and the like. ] Can be mentioned.

In the composition of the present invention, the nonionic surfactant and / or the amphoteric surfactant may be selected from the above alone or in combination of two or more. For example, in the composition of the present invention, the polyoxyethylene alkyl ether has an alkyl group having 12 to 15 carbon atoms and 7 to 12 mol of added ethylene oxide, and has 9 to 11 carbon atoms. Alkyl groups, those having an added 7-12 mol of ethylene oxide, and alkyl groups having 12-14 carbon atoms, those having an added 7-12 mol of ethylene oxide, and alkylpoly. As the glucoside, a combination with caprylyl / capryl glucoside can be preferably used.

In the composition of the present invention, the nonionic surfactant and / or the amphoteric surfactant is contained in an amount of 1 to 20% by mass, preferably 5 to 15% by mass, more preferably 7 to 12% by mass, still more preferably 9. It can be included in an amount set in the range of ~ 12% by mass. If the amount of the nonionic surfactant and / or the amphoteric surfactant is small, the cleaning effect may not be obtained or the effect may be poor. On the other hand, if the amount of the nonionic surfactant and / or the amphoteric surfactant is too large, the object may become slimy and sticky after use, which is not preferable.

As the alkaline agent (component (C)) used in the composition of the present invention, those generally used for antibacterial cleaning agents can be used, and inorganic alkaline substances (for example, alkali metal hydroxides and alkali metals) can be used. Examples thereof include silicates, alkali metal carbonates, alkali metal hydrogen carbonates, etc.), but the present invention is not limited thereto. Preferably, sodium hydroxide, potassium hydroxide, sodium silicate, potassium silicate, sodium carbonate, potassium carbonate, sodium hydrogencarbonate, potassium hydrogencarbonate can be utilized. In the composition of the present invention, the alkaline agent selected from the above may be used alone or in combination of two or more. For example, in the composition of the present invention, an alkali metal hydroxide can be preferably used, and in particular, a combination of sodium hydroxide and potassium hydroxide can be preferably used.

In the composition of the present invention, the alkaline agent can be contained in an amount such that the pH of the composition is 11 or more, preferably 12 or more (at 25 ° C.). More preferably, the pH of the composition of the present invention exceeds 12.1 (for example, 12.2 or more, 12.3 or more, 12.4 or more, 12.5 or more), and more preferably 13 or more. Can be included in quantity. The upper limit of pH is not particularly limited, but can be less than 14. In order to make the pH of the composition of the present invention 11 or more, the amount of the alkaline agent blended in the composition of the present invention is, for example, 0.07 to 10% by mass, 1 to 10.0% by mass, 5 to 10.0% by mass, 7 to 10.0% by mass, 8 to 10.0% by mass, or 8.5 to 10.0% by mass can be exemplified, and can be appropriately selected from these ranges (10.0% by mass). These ranges are not limited). If the amount of the alkaline agent is small, the cleaning effect may be poor, and the pH of the composition may not be maintained at 11 or higher in the presence or absence of organic stains.

Examples of the water (component (D)) used in the composition of the present invention include tap water, soft water, ion-exchanged water, pure water, purified water and the like, but from the viewpoint of storage stability of the composition, soft water. , Ion-exchanged water, pure water, purified water and the like are preferably used.

In the composition of the present invention, water is blended in the remaining mass% as an amount necessary for the sum of water and other components to reach 100% by mass.

The composition of the present invention can be further appropriately blended with a chelating agent and / or a water-soluble solvent as long as the virus inactivating effect of the present invention is not impaired.

As the chelating agent, those generally used for antibacterial cleaning agents can be used, and examples thereof include at least one selected from hydroxycarboxylic acid and its salt, and aminocarboxylic acid and its salt. Examples of the hydroxycarboxylic acid include citric acid, malic acid, gluconic acid, succinic acid, tartrate acid, lactic acid and the like, and examples of the aminocarboxylic acid include methylglycine diacetic acid, ethylenediaminetetraacetic acid, nitrilotriacetic acid, diethylenetriaminepentaacetic acid and hydroxyethyl. Examples thereof include ethylenediaminetetraacetic acid. Examples of these salts include alkali metal salts, alkaline earth metal salts, ammonium salts, alkanolamine salts and the like. Preferably, citric acid, sodium citrate, potassium citrate, sodium gluconate, sodium malate, sodium tartrate, and sodium lactate can be used from the viewpoint of easy availability, detergency, and storage stability. .. In the composition of the present invention, the chelating agent selected from the above may be used alone or in combination of two or more. For example, sodium gluconate can be preferably used in the composition of the present invention.

In the composition of the present invention, the chelating agent can be set in the range of 0.1 to 5% by mass, preferably 0.5 to 3% by mass. If the amount of the chelating agent is small, the detergency and the storage stability of the composition may be poor, while if the amount of the chelating agent is too large, the detergency improving effect derived from the chelating agent becomes saturated. Rather, it may be economically disadvantageous.

As the water-soluble solvent, those generally used for antibacterial detergents can be used, and examples thereof include at least one selected from glycol ether solvents and alcohols.

Examples of the glycol ether-based solvent include ethylene glycol ethers (for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, ethylene glycol monobutyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, and ethylene glycol dipropyl ether). , Ethylene glycol dibutyl ether, etc.), Diethylene glycol ethers (eg, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, diethylene glycol dibutyl ether, etc.), propylene glycol ethers (eg, propylene glycol monomethyl). Ether, propylene glycol monobutyl ether, propylene glycol monophenyl ether, propylene glycol dimethyl ether, propylene glycol dibutyl ether, etc.), dipropylene glycol ethers (for example, dipropylene glycol monomethyl ether, dipropylene glycol monobutyl ether, dipropylene glycol dimethyl ether, di Examples thereof include propylene glycol dibutyl ether, etc.), alkylene glycols (for example, propylene glycol, polyethylene glycol, etc.) and the like.

Examples of the alcohol include methyl alcohol, ethyl alcohol, propyl alcohol, isopropyl alcohol, butyl alcohol, methoxybutyl alcohol, benzyl alcohol, 3-methyl-3-methoxybutanol and the like.

Preferably, diethylene glycol ethers and dipropylene glycol ethers can be used from the viewpoints of easy availability, rinsability, permeability to oil stains, and solubility of stains.

In the composition of the present invention, the water-soluble solvent selected from the above may be used alone or in combination of two or more. For example, in the composition of the present invention, a combination of diethylene glycol monobutyl ether and dipropylene glycol monomethyl ether can be preferably used.

The composition of the present invention may contain a water-soluble solvent in an amount set in the range of 5 to 20% by mass, preferably 7 to 15% by mass, and more preferably 7 to 10% by mass. If the amount of the water-soluble solvent is small, the cleaning effect, particularly the permeability to oil stains and the solubility of the stains, may be poor, while if the amount of the water-soluble solvent is too large, the composition is stored. It may be less stable.

Further, the composition of the present invention includes further other additives as required, such as skin irritation relievers, dyes, fragrances, preservatives, metal corrosion inhibitors, hop extracts, bactericides such as polylysine, and the like. The surfactant of the above can be appropriately blended as long as the virus inactivating effect of the present invention is not impaired. Examples of the skin irritation relieving agent include aloe extract, hechima extract, perilla extract, sage extract, glycerin, and sorbitol, which have a moisturizing effect.

In the composition of the present invention, when each component is a liquid, it is generally mixed and stirred, and when it contains a solid component, it is generally dissolved in water first, and then other liquid components are added and mixed and stirred. However, depending on the composition, the production procedure such as addition order, dissolution order, and heating / cooling performed as necessary is not particularly limited.

The pH of the composition of the present invention (JIS Z-8802: 2011 "pH measuring method") is in the range of 11 or more, preferably 12 or more at 25 ° C. By setting the pH of the composition of the present invention to 11 or higher, it is possible to exert an inactivating effect on the virus. More preferably, the pH of the composition of the invention (JIS Z-8802: 2011 "pH measuring method") exceeds 12.1 at 25 ° C. (eg, 12.2 or higher, 12.3 or higher, 12.4). (12.5 or more), more preferably 13 or more. By setting the pH of the composition of the present invention to a range exceeding 12.1, the pH of the composition is inactivated by norovirus even in the presence of organic stains, that is, in contact with or mixed conditions with organic stains. It can be maintained at pH 11 or higher, which is effective for the invention. Thereby, the composition of the present invention can retain the inactivating effect on norovirus even in the presence of organic stains. The pH of the composition of the present invention can be adjusted in the above range by using the above alkaline agent, preferably an alkali metal hydroxide, more preferably a combination of sodium hydroxide and potassium hydroxide.

As used herein, the term "organic stain" means deposits or contaminants of a composition containing a large amount of protein components (for example, feces, vomit, food, etc.).

The virus can be inactivated by cleaning the object to be cleaned to which the virus has adhered using the composition of the present invention. Examples of the virus that can be inactivated using the composition of the present invention include calicivirus, preferably norovirus.

As long as the composition of the present invention has the above pH value and has an inactivating effect on a virus, it is appropriately diluted, for example, 2-fold to 200-fold, 3-fold to 150-fold, 10-fold to 100-fold, and the like. May be used. Dilution can be done with water.

The object to be washed using the composition of the present invention can be washed by the following method.

(1) The composition of the present invention can be impregnated into a non-woven fabric to form an anti-virus cleaning material (hereinafter, may be referred to as "anti-virus cleaning material of the present invention"), which can be used to inactivate viruses. By wiping the object to be cleaned, the virus can be inactivated and the object to be cleaned can be cleaned. Items to be cleaned include medical sites, nursing sites, food factories, coffee shops, restaurants, hotels, taverns, schools (school lunch), central kitchens, kitchens such as supermarket backyards, refrigerators, storages, and tables. Examples include desks, doorknobs at doorways, toilet bowls and seats, water faucets, and those with hard surfaces such as floors and walls. In addition, the antiviral cleaning material of the present invention can also be used as a wet towel, a wet tissue, or the like. In particular, according to the anti-virus cleaning material of the present invention, even if the object to be cleaned is contaminated with organic stains, the virus is inactivated and the object to be cleaned by wiping without reducing or losing the inactivating effect of the virus. Can be cleaned.

Examples of the raw material of the non-woven fabric constituting the anti-virus cleaning material of the present invention include cellulosic materials such as cotton, protein-based materials such as wool or silk, and chemically polymerized materials such as rayon, polyester and acrylic. Of these, cellulosic materials and chemically polymerized materials are preferable.

(2) The composition of the present invention can be used by an appropriate method in addition to the above. For example, the surface of the object to be cleaned is sprayed with a spray or the like, left for about 1 to 5 minutes after a predetermined time, rinsed with water as appropriate, and then dried. Specifically, by using a dedicated dispenser containing the composition of the present invention and spraying it on the surface of the object to be cleaned at a rate of about 6 to 12 ml / m ² each time it is used, virus-free. Along with activation, the object to be cleaned can be washed. Examples of the object to be cleaned include the above-mentioned items. In particular, according to the composition of the present invention, even if the object to be cleaned is contaminated with organic stains, the virus is inactivated and the substance to be washed is inactivated by spraying without reducing or losing the inactivating effect of the virus. Can be washed.

The composition of the present invention is provided by being filled in a plastic container, a container with a pump, a pouch, a tube, or the like. In addition, it can be individually packaged in a considerable amount used each time and provided with portability.

Hereinafter, the present invention will be specifically described with reference to Examples, but the present invention is not limited thereto.

I. Preparation of Antiviral Detergent Composition According to the composition shown in Table 1 below, each component was mixed to prepare an antiviral detergent composition of Composition 1-6. The obtained antiviral detergent composition was a yellow transparent liquid.

The polyoxyethylene alkyl ether is used in combination of three types, and the polyoxyethylene alkyl (C12-15) ether is added with an alkyl group having 12 to 15 carbon atoms and 7 to 12 mol of ethylene oxide. The polyoxyethylene alkyl (C9-11) ether is composed of an alkyl group having 9 to 11 carbon atoms and 7 to 12 mol of ethylene oxide added to the polyoxyethylene alkyl (C9-11) ether. C12-14) Ether indicates that an alkyl group having 12 to 14 carbon atoms and 7 to 12 mol of ethylene oxide are added.

In Table 1, the amount of each component is shown by mass%.

The storage stability of each antiviral detergent composition was evaluated. Each antiviral detergent composition was placed in a 250 mL plastic container and stored at room temperature for 1 month, and then the appearance of each composition was visually observed and evaluated.
As a result, the compositions 1, 2, 5 and 6 showed good storage stability, and no precipitation or cloudiness was observed in the composition. On the other hand, in the compositions 3 and 4, precipitation, cloudiness, separation and the like were partially confirmed.

Each antiviral detergent composition was diluted 100-fold with water (100-fold diluted solution) and used in the following inactivation test. The pH was measured at 25 ° C. according to JIS Z-8802: 2011 “pH measuring method” (Table 1).

II. Feline calicivirus (FCV) inactivation test 1. Test method Using FCV, which is a substitute virus for norovirus, a test for determining the norovirus inactivating effect of the antivirus detergent composition prepared in the above "I. Preparation of antivirus detergent composition" was conducted. The test method is a known method (Food Hygiene Inspection Guideline Microorganisms Edition (2015) "Virus Inactivation Test";"2015 Survey Report on Norovirus Inactivation Conditions" National Institute of Health Sciences Food Hygiene Management Department, etc.) The method was the same.

That is, the virus solution (FCV F9 strain) and each anti-virus detergent composition (100-fold diluted solution) were mixed at a ratio of 1: 9, left at room temperature for 30 seconds, and then DMEM medium supplemented with 2% FBS. The reaction was stopped after diluting 7-fold with. Then, 7-fold serial dilutions were performed for each, and each diluted solution was inoculated into cat kidney-derived cells (CRFK cells), and the amount of viable virus was quantified by the 50% infection terminal point method (TCID ₅₀ / ml).

In order to carry out an organic load test assuming organic stains, an equal amount of MEM medium containing 1% meat extract (Nakalitesk) and a virus solution were mixed to prepare a virus solution containing 0.5% meat extract. The same operation as above was performed on this virus solution to quantify the amount of viable virus.

In determining the efficacy, the virus was diluted 7-fold stepwise after the reaction, so the measured value (log ₇ ) was converted to log ₁₀ , and the determination was made based on the converted value. The inactivating effect is determined based on the amount of decrease in infectious titer, and according to the difference in infectious titer (value converted to log ₁₀ ) from the inoculated virus, A: Sufficient effect (amount of decrease in infectious titer of 4 log ₁₀ or more), B. : Effective (2log ₁₀ or more and less than 4log ₁₀ infectious titer reduction amount), and C: No effect (2log ₁₀ or less infectious titer reduction amount).

As a control, a judgment test was conducted using a commercially available alkaline disinfectant cleaning agent (Products A, B and C) in the same manner. Products A and B contain benzalkonium chloride as an active ingredient, and products C contain a dialkyldimethylammonium salt as an active ingredient.

2. 2. Results The results of the FCV inactivation test are shown in Table 2 below.
It was confirmed that all of the antiviral detergent compositions of Composition 1-5 exhibited an inactivating effect on the virus under the condition of no load of organic substances. On the other hand, it was confirmed that the antiviral detergent composition of Composition 6 does not show a virus inactivating effect.

Further, it was confirmed that the antiviral detergent composition of Composition 1-4 exhibits an inactivating effect on the virus under the condition of loading with an organic substance. On the other hand, it was confirmed that the antiviral detergent compositions of Compositions 5 and 6 did not show a virus inactivating effect.

Regarding the commercially available alkaline disinfectant detergents (Products A, B and C), only Product A showed an inactivating effect on the virus under the condition without organic matter load, but under the condition with organic matter load. It was confirmed that none of the products showed a virus inactivating effect.

The above results show that any of the anti-virus cleaning agent compositions of Composition 1-5 can inactivate norovirus, and in particular, the anti-virus cleaning agent compositions of Compositions 1, 2, 3 and 4. Indicates that the effect is not lost even in the presence of organic stains. On the other hand, according to Product A, commercially available disinfectant cleaners can inactivate norovirus in the absence of organic stains, but in the presence of organic stains, all products use norovirus. Indicates that it cannot be inactivated. When the pH value of the reaction solution is less than 11, the pH value of the reaction solution is less than 11 due to the relationship between the pH values of the compositions 6 and products B and C having no virus inactivating effect and the presence or absence of these virus inactivating effects. It was shown that the virus inactivating effect could not be obtained.

III. Detergency test (1) Burn-in oil stain 1. Test method Assuming oil stains stuck around the range fryer in the kitchen, apply about 1.0 mL of soybean oil (Kanto Chemical Co., Inc., 37308-02) to the stainless steel (SUS-304, 26 mm x 76 mm) test piece. Then, it was baked in a constant temperature device (manufactured by Yamato Scientific Co., Ltd., precision constant temperature device DH412) set at 180 ° C. for 1 hour or 3 hours.

200 mL of each anti-virus cleaning agent composition of Composition 1-6 prepared in the above "I. Preparation of anti-virus cleaning agent composition" and commercially available alkaline disinfectant cleaning agents (products A, B and C). , 200 mL beakers were placed in each, and three test pieces prepared by the above method were immersed in the beakers for 10 minutes.

After 10 minutes, the test piece was taken out, rinsed lightly with tap water, the antiviral detergent composition was removed and dried, and then the cleaning rate was calculated from the following formula.
Cleaning rate (%) = {(W1-W2) / (W1-W0)} × 100
W0: Weight of stainless steel test piece before baking soybean oil (g)
W1: Weight after baking soybean oil on a stainless steel test piece (g)
W2: Weight (g) of a stainless steel test piece baked with soybean oil after being immersed in a test solution and dried.

2. 2. Results The measurement results of the detergency of the antiviral cleaning agent composition against seized oil stains are shown in Table 3 below. The results in Table 3 show the average value (n = 3) in each test. In Table 3, "A" indicates a cleaning rate of 85% or more, "B" indicates a cleaning rate of 60% or more and less than 85%, and "C" indicates a cleaning rate of less than 60%.

From this result, it was confirmed that each anti-virus cleaning agent composition of Composition 1-5 has a cleaning power comparable to that of a commercially available alkaline disinfectant cleaning agent against baking oil stains for 1 hour. Was done. Further, each of the anti-virus cleaning agent compositions of Compositions 1 and 2 has higher detergency than other compositions 3-6 and commercially available alkaline disinfectant cleaning agents against baking oil stains for 3 hours. It was confirmed to have.

(2) Oil stains 1. Test method Assuming oil stains on the floor, edible oil (about 3 mL) colored with carbon black in an amount of × 10, × 50 or × 100 is applied to the surface of a white vinyl tile (30 cm × 30 cm). And dried at room temperature for 24 hours. This tile was cut into 7.5 cm x 4 cm and used as a test piece.

For each test piece, each anti-virus cleaning agent composition of Composition 1-6 prepared in the above "I. Preparation of anti-virus cleaning agent composition", and a commercially available alkaline disinfectant cleaning agent (Product A, 4.5 mL of B and C) was added dropwise.

Next, it was installed in a washability tester (manufactured by Tester Sangyo), the sponge was reciprocated 10 times, washed, and then lightly rinsed with tap water.

The brightness of the tile surface at each stage was measured using a color difference meter (manufactured by Konica Minolta), and the cleaning rate was calculated from the following formula.
Cleaning rate (%) = {(L2-L1) / (L0-L1)} × 100
L0: Brightness of tiles before applying carbon black stains L1: Brightness of tiles after applying carbon black stains L2: Brightness of tiles after cleaning test

2. 2. Results The measurement results of the detergency of the antiviral detergent composition against oil stains are shown in Table 4 below. The results in Table 4 show the average value (n = 2) in each test. In Table 4, "A" indicates a cleaning rate of 85% or more, "B" indicates a cleaning rate of 60% or more and less than 85%, and "C" indicates a cleaning rate of less than 60%.

From this result, it was confirmed that each anti-virus cleaning agent composition of Composition 1-5 has a detergency comparable to that of a commercially available alkaline disinfectant cleaning agent against oil stains.

From the above results, it was confirmed that the antiviral detergent compositions of Compositions 1, 2, 3, 4, and 5 have an excellent inactivating effect on norovirus. In particular, the antiviral detergent compositions of Compositions 1, 2, 3 and 4 retained an excellent inactivating effect on norovirus even in the presence of organic stains. Furthermore, it was confirmed that the antiviral detergent compositions of Compositions 1 and 2 have particularly good properties in terms of storage stability and detergency.

Claims (9)

The following components (A) to (C), a chelating agent and a water-soluble solvent are contained in the following ratio with respect to the entire composition, water is contained as the component (D), and the pH of the composition (JIS Z-). 8802: 2011 "pH measuring method") is characterized by exceeding 12.1 at 25 ° C.:
(A) Quaternary ammonium salt 0.5-5% by mass
(B) Nonionic surfactant and / or amphoteric surfactant 1 to 20% by mass,
(C) Alkaline agent 0.07 to 10.0% by mass,
Chelating agent 0.1-5% by mass,
Water-soluble solvent 5 to 20% by mass,
The nonionic surfactant and / or amphoteric surfactant is a polyoxyalkylene alkyl ether and an alkyl polyglucoside.
The chelating agent is a hydroxycarboxylic acid and a salt thereof.
The water-soluble solvent contains dipropylene glycol ethers . The antiviral cleaning agent composition according to claim 1, wherein the quaternary ammonium salt is benzalkonium chloride, benzethonium chloride or didecyldimethylammonium chloride. The antiviral detergent composition according to claim 1 or 2, wherein the polyoxyalkylene alkyl ether is a polyoxyethylene alkyl ether . The antiviral detergent composition according to any one of claims 1 to 3, wherein the alkaline agent is at least one selected from inorganic alkaline substances. The antiviral detergent composition according to any one of claims 1 to 4, wherein the chelating agent is sodium gluconate . The antiviral detergent composition according to any one of claims 1 to 4, wherein the water-soluble solvent contains dipropylene glycol monomethyl ether . An antiviral cleaning material, which comprises impregnating a non-woven fabric with the antiviral cleaning agent composition according to any one of claims 1 to 6. A method for using an anti-virus cleaning material according to claim 7, wherein the anti-virus cleaning material is used for inactivating a virus on the surface of an object to be cleaned except for human skin. A method for inactivating a virus, which comprises spraying the antiviral detergent composition according to any one of claims 1 to 6 onto the surface of an object to be cleaned except human skin.