JP6444104B2 - Liquid sterilizing detergent composition for washing and method for inactivating non-enveloped viruses in textiles using the same - Google Patents

Description

  The present invention relates to a liquid sterilizing detergent composition for laundry having inactivation properties against non-enveloped viruses and a method for inactivating non-enveloped viruses in textiles using the same.

  One of the reasons for difficulties in hygiene management measures at food production and cooking facilities, medical facilities, and nursing homes is food poisoning caused by the caliciviridae norovirus. In FY2006, the incidence of food poisoning due to norovirus is the highest in both the number of cases and the number of patients, and large-scale food poisoning has also occurred due to its strong infectivity. In addition, the cause of food poisoning due to norovirus was said to be eating bivalves such as oysters, but recently, norovirus contained in food, feces and vomit is infected from person to person, Furthermore, it has been found that secondary contamination such as spreading due to contact with a clean surface via contaminated food or water is the main cause. To prevent this secondary contamination, thoroughly wash hands, sterilize cutting tools and utensils such as cutting boards and knives, sterilize cooking tables and work tables, wash toilet door knobs and toilet seats, and medical equipment, and adhere to clothing, etc. The treatment method of the vomit and excrement which became good becomes important.

  In particular, nursing clothes worn by those who need nursing care and clothes such as diapers for newborns are contaminated with a large amount of excrement such as urine and stool, and bacteria and viruses contained in the excrement are By breeding inside, it forms extremely unsanitary dirt. With respect to such stains, normal liquid detergents intended for sebum stains, mud stains, etc. cannot be sufficiently sterilized and washed. Has been developed.

  For example, by blending (a) a surfactant, (b) ethanolamine, (c) a chelating agent, and (d) a water-soluble carbonate at a specific ratio, urine and feces peculiar to baby clothing Has disclosed a liquid detergent composition that is intended to improve the cleaning effect on both soils derived from sewage and soils attached to normal clothes such as food soil, sebum soil, and mud soil (see Patent Document 1). ).

  Further, (A) a surfactant obtained by mixing one or more of an anionic surfactant, an amphoteric surfactant and a nonionic surfactant, and (B) one or two of a specific aminopolycarboxylic acid and a salt thereof. Improve the cleaning effect against dirt peculiar to underwear (bilirubin dirt) by blending chelating agent mixed with more than one species and (C) alkaline agent commonly used in conventional detergents at a specific ratio An intended cleaning agent is disclosed (see Patent Document 2).

  In addition, sterilization and softening agent composition that can be used as sterilization and softening treatment of textile products such as squeezes, towels, diapers, sheets, yukata and pajamas, nightwear, underwear, and paper, etc. A composition containing polyaminomonocarboxylic acid, benzalkonium chloride and a silicone-based texture improver as main components is disclosed (see Patent Document 3).

  By the way, Norovirus is an RNA-type virus that has only RNA, and is a non-enveloped virus that is surrounded by a protein shell called capsid and does not have a membrane made of sugar and lipid called envelope. For this reason, like viruses having an envelope, there is a problem that the envelope is easily destroyed by the drug, cannot be bound to the host cell receptor and is not inactivated, and it is difficult to inactivate by the drug.

  Currently, methods for inactivating norovirus include a method using a sodium hypochlorite solution and a method using hot water disinfection (see Non-Patent Document 1). However, in hot water disinfection, it is desirable to maintain a temperature of 85 ° C. or higher. However, if hot water is applied to the object to be disinfected, the temperature decreases due to contact with the object to be disinfected, and the desired temperature can be maintained. It is difficult and there is a risk of burns, making it unsafe.

  Also, in the disinfection method using sodium hypochlorite solution, depending on the disinfectant, it may be decolorized due to the bleaching action / effect of sodium hypochlorite, and the workplace may be filled with chlorine odor. There is also.

  Furthermore, alcohol is generally used for sterilization of cooking utensils such as cutting boards and kitchen knives, but alcohol has a poor inactivation effect on feline calicivirus F9, which is an alternative virus for norovirus. Is known (see Non-Patent Documents 2 and 3).

  Further, it is known that a disinfectant containing a cationic surfactant exhibits an effect on food poisoning bacteria but has no inactivation effect on viruses (Non-patent Document 3). And 4).

  On the other hand, there is also a report that virus inactivation is observed when a cationic surfactant is used and an alkali agent is added (see Non-Patent Documents 5 and 6).

  In addition, there is a report that the combination of ethanol, alkaline substance and cationic fungicide is effective in inactivating feline calicivirus and can be expected to be applied to the disinfection of hard surfaces contaminated with virus-containing microorganisms (non- (See Patent Document 7).

  Further, a disinfecting solution containing 0.05 to 0.5% by weight of a polyhexamethylene biguanide compound and having a pH in the range of 9 to 12, comprising benzalkonium chloride, cetylbiridinium chloride, phenoxyethanol In addition, disinfecting solutions and disinfecting articles that can contain other antibacterial components such as chlorohexidine gluconate and lauryl sulfate and have a norovirus-inactivating effect gentle to the skin are disclosed (see Patent Document 4).

Japanese Patent Laid-Open No. 11-5997 Japanese Patent Laid-Open No. 63-6097 Japanese Patent No. 2506125 JP 2007-45732 A

“Norovirus Food Poisoning Countermeasures (Proposal)” Food and Drug Hygiene Council Food Hygiene Division Food Poisoning Department (October 12, 2007) “Inactivation of Caliciviruses”, American Society for Microbiology, Vol. 70, no. 8, 4538-4543 (2004) “Inactivation of feline calicirus, a Norwalk virus surrogate”, J. Am. Hosp. Infect, 41, 51-57 (1999) “Virtual Disinfectants and Feline Viruses”, Am. J. et al. Vet. Res. Vol. 41, no. 3, 410-414 (1980) "Efficacy of common used discovered for the inactivation of calicirus on strawberry, lettuce, and food-contact surface", J. Am. Food. Prot. 64, 1430-1434 (2001) “Effects of quarterly ammonium compounds with 0.1% sodium hydroxide on sine vesicular disease virus”, J. Am. Vet. Med. Sci. 59,323-328 (2007) “Development of norovirus-replacement feline calicivirus and ethanol preparation effective for various microorganisms”, antibacterial and antifungal, Vol. 35, no. 11, 725-732 (2007)

  However, the technical contents and compositions described in these documents only satisfy specific performances, and are limited to evaluation and use in a stock solution or in a high concentration. Even if it is applied to detergents for washing, the actual situation is that a satisfactory effect is not obtained.

  The present invention has been made in view of such circumstances, and has a combination of a specific quaternary ammonium salt, an alkali agent and a chelating agent, has inactivation against non-enveloped viruses even at low concentrations, and excellent sterilization. And providing a method for inactivating non-enveloped viruses in textiles using the same, and also providing a liquid sterilizing detergent composition for washing with less soil reattachment to textiles. Objective.

In order to achieve the above-mentioned object, the present invention provides a fiber product to which a non-enveloped virus is attached in a washing machine for sterilization, washing, and inactivation of non-envelope virus. It is a liquid sterilizing detergent composition for washing used in a state diluted to a concentration range of mass%, and the following (A) to (C) components are contained in the following proportions, A liquid sanitizing detergent composition for laundry having a pH at 25 ° C. of a mass% aqueous solution in the range of 10.5 to 11.5 and having inactivation to non-enveloped viruses is a first gist.
(A) Quaternary ammonium salt 1 to 10 wt% a A Le kill dimethyl benzyl ammonium chloride Rye de.
(B) Alkali agent which is at least one selected from the group consisting of alkali hydroxide, carbonate, silicate and alkanolamine, and contains at least one of alkali hydroxide, carbonate and silicate. mass%.
(C) Citric acid, gluconic acid, succinic acid, tripolyphosphoric acid, hydroxyethylethylenediaminetriacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, nitrilotriacetic acid, triethylenetetraminehexaacetic acid, phosphonic acids, L-glutamic acid diacetic acid, methylglycine diacid 3 to 10 % by mass of a chelating agent which is at least one selected from the group consisting of acetic acid and alkali metal salts thereof .

  In addition, the present invention is a liquid sterilizing detergent composition for laundry, particularly containing 1 to 30% by mass of a nonionic surfactant as the component (D) together with the components (A) to (C). Is the second gist.

In addition, the present invention has, as a third aspect, a liquid sterilizing detergent composition for washing, in which the non-enveloped virus is a caliciviridae virus, among them, in particular, the caliciviridae in particular. A fourth aspect is a liquid sterilizing detergent composition for washing, in which the virus is a Norovirus genus virus.

Furthermore, this invention dilutes the liquid sterilization detergent composition for washing | cleaning which is the summary of any one of the said 1st- 4th so that it may become the density | concentration range of 0.1-0.5 mass%, and is used as a washing liquid. Used to inactivate non-enveloped viruses in textiles by washing the textiles with non-enveloped viruses on a washing machine to inactivate the non-enveloped viruses as well as sterilize and wash the textiles. The method is the fifth gist.

That is, the present inventors have found, after intensive studies in order to solve the above problems, combined with A Le kill dimethyl benzyl ammonium chloride Lai de, and certain alkaline agent, and a specific chelating agent, 0 at 25 ° C. By adjusting the pH of a 1% by weight aqueous solution to the range of 10.5 to 11.5, it has an inactivating effect against non-enveloped viruses, exhibits sufficient sterilizing power and detergency, and has fibers The present inventors have found that a liquid sterilization detergent composition for washing is obtained that is less likely to cause redeposition of dirt to the article and that causes little damage to the textile article.

  The liquid sterilizing detergent composition for washing having inactivation properties against non-enveloped viruses of the present invention, as described above, has inactivation properties against non-enveloped viruses and exhibits excellent disinfection and cleaning effects. There is an advantage that there is little recontamination of dirt on textiles such as clothing. Therefore, vomit, urine, stool, and other clothing, squeezes, towels, sheets, and nursing clothes worn by those in need of nursing care, clothing for newborns, etc. should be hygienically washed once. And it can be finished cleanly.

  And especially in this invention, the liquid sterilization detergent composition for washing | cleaning which contains 1-30 mass% of nonionic surfactant as (D) component with said (A)-(C) component, It is preferable because it has a much better cleaning effect.

  Among the present invention, in particular, those in which the non-enveloped virus is a caliciviridae virus, in particular, those in which the caliciviridae virus is a norovirus virus are particularly suitable because of their particularly high inactivation performance against viruses. is there.

  Further, according to the method for inactivating non-enveloped viruses in textiles according to the present invention, the washing liquid sterilization washing is performed in the same manner as in the case of general washing using a household or business washing machine. The non-enveloped virus can be inactivated simply and effectively by the agent composition.

  Next, a mode for carrying out the present invention will be described in detail.

Washing liquid germicidal detergent composition of the present invention (hereinafter, referred to as "the composition") is, A Le Kill dimethyl benzyl ammonium chloride of at least one in which quaternary ammonium salts and (A component), a particular alkaline agent ( B component) and a specific chelating agent (C component), and the pH at 25 ° C. of a 0.1 mass% aqueous solution (JIS Z-8802: 1984 “pH measurement method”) is 10.5 to 11.5. Is set in the range of.

(A) above is used as component luer Le kills dimethyl benzyl ammonium chloride, the textiles to be wash target material (cotton, polyester, nylon, etc.) without depending on, for any fiber at the time of washing Because it is hardly adsorbed, it contributes to the inactivation of non-enveloped viruses adhering to the surface to be cleaned of fibers and the removal of bacteria, and also contributes to the effect of finishing with less re-contamination of fibers. To do. It is also excellent in that it is easily available.

The component (A) may be used alone or in combination of two or more . Their to, among the above alkyl dimethyl benzyl ammonium chloride, especially if the number of carbon atoms in the alkyl group with not more than 10, it is particularly excellent in anti-redeposition performance relative to the textile, preferably It is. Therefore, it is particularly preferable to use decyldimethylbenzylammonium chloride (alkyl group having 10 carbon atoms) or octyldimethylbenzylammonium chloride (alkyl group having 8 carbon atoms).

  And the compounding quantity of the said (A) component must be set in the range of 1-10 mass% with respect to this composition whole. That is, when the blending amount is less than 1% by mass, the effect of inactivating non-enveloped viruses and removing bacteria is poor. This is because the amount of recontamination increases. Among the above ranges, it is particularly preferable to set a range of 3 to 8% by mass from the viewpoints of inactivation of non-enveloped viruses, removal of bacteria, and prevention of soil recontamination.

Moreover, the alkaline agent of the component (B) used in the present invention contributes to the inactivation of non-enveloped viruses adhering to the surface to be cleaned of the fiber, the improvement of the removal action against bacteria, and the cleaning effect on the textile. Examples of the component (B) include alkali hydroxides, carbonates, silicates, and alkanolamines. These may be used alone or in combination of two or more , but must contain at least one of an alkali hydroxide, a carbonate and a silicate .

  The compounding quantity of the said (B) component must be set in the range of 1-20 mass% with respect to this composition whole. That is, when the blending amount is less than 1% by mass, the non-enveloped virus inactivation effect, sterilization effect and cleaning effect are poor, and when it exceeds 20% by mass, the non-enveloped virus inactivation effect, sterilization effect and cleaning effect are poor. This is because it becomes saturated, which is economically disadvantageous, and damage to the textile is increased. Among the above ranges, it is particularly preferable to set the content in the range of 5 to 15% by mass from the viewpoint of improving the inactivation effect of the non-enveloped virus and the sterilization effect, and from the point of damage to the textiles.

Furthermore, the chelating agent of component (C) used in the present invention contributes to an improvement in the inactivation effect of non-enveloped viruses, an improvement in the sterilization effect against bacteria, and a washing effect on textiles. Examples of the component (C) include citric acid, gluconic acid, succinic acid, tripolyphosphoric acid, hydroxyethylethylenediaminetriacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, nitrilotriacetic acid, triethylenetetraminehexaacetic acid, phosphonic acids, and L-glutamic acid diacid. Acetic acid, methyl glycine diacetic acid, and alkali metal salts thereof are used . You may use these individually or in combination of 2 or more types.

The amount of the component (C), the overall composition, shall set within a range of 3 to 10 wt%. That is, inactivating effect of non-enveloped viruses in amount of less than 1% by weight, poor effect of improving the sterilizing effect and cleaning effect, more than 20 wt% state, and are from is economically disadvantageous, in particular, 3 can be set within a range of 10 wt%, improvement in sterilizing effect, it is preferable So in terms of cleaning efficacy and economy.

Further, the composition pH at 25 ° C. of 0.1 wt% aqueous solution (JIS Z-8802: 1984 "pH Measurement Methods") must be set in the range of 10.5 to 11.5. In less than pH 10.0, inactivating effect of non-enveloped viruses, poor sterilization effect, exceeding 12.0, and than ing large damage to the textile, especially Among them, 10.5 Setting in the range of 11.5 is necessary from the viewpoint of improving the inactivation effect of the non-enveloped virus and reducing the damage to the textile.

A laundry liquid remover having inactivation of non-enveloped viruses containing the above components (A) to (C) and having a 0.1 mass% aqueous solution having a pH at 25 ° C. set in the range of 10.5 to 11.5 The fungus cleaning composition exhibits excellent non-envelope virus inactivation, sterilization and cleaning effects against unhygienic soil formed by vomit, urine and feces, and textiles such as clothing This has the advantage that there is little recontamination of dirt on the fabric and has the advantage that there is little damage to the textile. Therefore, clothes that have vomit, etc., squeezes, towels, sheets, as well as nursing clothes worn by those who need nursing care, and textiles such as newborn clothes, are washed once. It can be sanitary and clean.

  In addition, when a nonionic surfactant is used as the component (D) together with the components (A) to (C) in the present composition, it is preferable that a further excellent effect can be obtained. The nonionic surfactant mainly contributes to the improvement of the cleaning effect. As the nonionic surfactant, for example, a linear or branched chain having 8 to 22 carbon atoms, preferably 12 to 18 carbon atoms is used. Polyoxyethylene alkyl ether, polyoxyethylene alkenyl ether, polyoxyethylene alkylene phenyl ether having an average addition mole number of ethylene oxide of 5 to 20, preferably 6 to 15 with respect to primary or secondary alcohol , Polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene polyoxypropylene alkenyl ether, polyoxyalkylene glycol fatty acid ester, alkylamine ethylene oxide adduct and the like. You may use these individually or in combination of 2 or more types.

  When using the said (D) component, it is preferable to set the compounding quantity in the range of 1-30 mass% with respect to this composition whole. That is, when the blending amount is less than 1% by mass, the effect of improving the cleaning effect on the fiber product is poor, and when it exceeds 30% by mass, the cleaning effect is saturated and may be economically disadvantageous. In addition, among the said range, setting to the range of 3-20 mass% especially is suitable from the point of the improvement of disinfection effect, a cleaning effect, and economical efficiency especially.

  Examples of the virus targeted for the liquid sterilizing detergent for washing having the inactivating effect of the non-enveloped virus of the present invention include single-stranded (+) RNA virus having no envelope, single-stranded (-) RNA virus, and two Examples include double-stranded RNA viruses, single-stranded DNA viruses, and double-stranded DNA viruses. Examples of these viruses include viruses belonging to the Caliciviridae, Picornaviridae, Parvoviridae, Papillomaviridae, Polyomaviridae, and Adenoviridae families.

  Among these, the virus inactivating effect of the present invention is excellent in the inactivating effect on Caliciviridae virus (single-stranded (+) virus), and belongs to the genera Norovirus and Sapovirus among the Caliciviridae viruses. Excellent inactivation effect against viruses, particularly excellent inactivation effect against viruses belonging to the genus Norovirus.

  In the present invention, “inactivation of a virus” means to remove or significantly reduce the infectivity and proliferation ability of a virus. Therefore, it is not always necessary to denature or disrupt virus particles or nucleic acids (RNA). Adsorption on the surface of viruses or host cells, binding of viruses and enzymes, and so on result in virus infectivity and proliferation ability. It suffices if it can be reduced or inhibited.

  Inactivation of the non-enveloped virus of the present invention can be achieved by using the composition in the same manner of use as a general laundry detergent, using household and commercial washing machines, in particular, It is desirable to use the present composition diluted to a concentration range of 0.1 to 0.5% by mass. That is, when a diluted solution in this concentration range is used for washing, in particular, inactivation performance and bacteria removal performance, and cleaning performance and recontamination prevention performance against non-enveloped viruses are exhibited in a well-balanced manner.

  In addition, the composition includes, as an optional component, a solvent such as propylene glycol and ethyl alcohol, a recontamination inhibitor, a pH adjuster, a viscosity adjuster, an enzyme, and an enzyme stabilizer as long as the effects of the present invention are not hindered. , Fluorescent brighteners, dyes, fragrances, deodorants, metal corrosion inhibitors and the like can be blended. In addition, even if the said solvent is added and contained in (A)-(D) component as a stabilizer, solubilizer, etc. of said (A)-(D) component, it is arbitrarily added separately. It may be done.

  In addition, this composition is normally prepared as a diluted liquid composition by adding an arbitrary component and water with respect to said (A)-(C) component or (A)-(D) component. Is done.

  Examples of the water include tap water, soft water, ion exchange water, pure water, purified water, and the like. Preferably, soft water, ion exchange water, and pure water are used. These may be used alone or in combination of two or more. And the remaining mass% is mix | blended with the said water as a balance so that the whole this composition may be 100 mass%.

  Hereinafter, examples of the present invention will be described in detail together with comparative examples. The present invention is not limited to these examples.

[Examples 1 to 29 , Reference Examples 1 to 11 , Comparative Examples 1 to 13]
A liquid sterilizing detergent composition for washing (hereinafter referred to as a test composition) having the inactivation properties of non-enveloped viruses having the compositions described in Tables 1 to 10 below is prepared, and its inactivation properties against feline calicivirus Each test item for sterilizing power against general bacteria, detergency, and soil recontamination prevention was tested and evaluated by the following methods. The result is combined with the said Table 1-Table 10, and is shown. In addition, the detail of each component in a table | surface is as follows, and the numerical value which shows the composition in a table | surface is shown by the effective content (mass%, pure part conversion) of each component.

[Component A]
* Quaternary ammonium salt 1
: Alkyl (C12-16) dimethylbenzylammonium chloride (manufactured by Clariant Japan / Dodigen 226)
* Quaternary ammonium salt 2
: Dioctyldimethylammonium chloride (Lonza Japan / Bardac LF 80)
* Quaternary ammonium salt 3
: Decyldimethylbenzylammonium chloride (Prototype 1)
* Quaternary ammonium salt 4
: Octyldimethylbenzylammonium chloride (Prototype 2)
* Quaternary ammonium salt 5
: Didecyldimethylammonium chloride (Lonza Japan / Bardac 2280)

[B component]
* Alkaline agent 1
: Sodium hydroxide (48% by mass caustic soda / Asahi Glass Co., Ltd.)
* Alkaline agent 2
: Sodium metasilicate pentahydrate (Sodium metasilicate pentahydrate crystal / Gourei Chemical Co., Ltd.)
* Alkaline agent 3
: Monoethanolamine (Nippon Shokubai Co., Ltd./monoethanolamine 90)

[C component]
* Chelating agent 1
: Tetrasodium ethylenediaminetetraacetate (BASF / Trilon B)
* Chelating agent 2
: Trisodium nitrilotriacetate (BASF / Trilon A92R)

[D component]
* Nonionic surfactant 1
: Polyoxyethylene alkyl ether, ethylene oxide 10 mol 8 mol adduct of C9-11 primary alcohol (manufactured by Gel Chemical Co., Ltd./Neodol 91-8)
* Nonionic surfactant 2
: Polyoxyethylene alkyl ether, 12-mole ethylene oxide adduct of C12-14 secondary alcohol (Nippon Shokubai Co., Ltd./Softanol 120)

[Inactivation test against feline calicivirus]
(Test method)
Each test composition was diluted to 0.15% by mass with tap water. After contacting 270 μl of the 0.15% by mass aqueous solution of this reagent and 30 μl of feline calicivirus F9 strain (FCV) for 10 minutes, α-Minimum Essential Medium (α-MEM) (manufactured by Wako Pure Chemical Industries, Ltd.) 10 times Was further diluted 10-fold in a stepwise manner. Then, 50 μl of α-MEM is added to feline kidney cells (CRFK) previously cultured in a 96-well microplate, and the previously diluted solution is added thereto, followed by the presence of 37 ° C., 5% CO _2. Under the condition, the cells were cultured for 4 days to confirm the presence or absence of cell degeneration. Moreover, the thing which used the ultrapure water instead of the reagent agent was made into the blank, and the presence or absence of cell degeneration was confirmed like the above. Then, the Log Reduction (logarithmic decrease value) of FCV by the reagent agent was calculated from these differences and evaluated according to the following criteria.

(Criteria)
○: Log Reduction is 4 or more.
Δ: Log Reduction is 2 or more and less than 4.
X: Log Reduction is less than 2.
Note that Δ and ○ are practical.

[Bacteria elimination test]
(Test method)
Escherichia coli (E. coli ATCC11229) was used as a bacterial model in the excrement, and the sterilizing power was evaluated by the following method. That is, first, each test composition was diluted to 0.15% by mass with 50 ppm (as CaCO ₃ ) hard water. Next, 100 μl of the bacterial solution adjusted so that the number of bacteria was 10 ⁸ was adhered to 10 g of fibers, and then the diluted solution and the bacteria-adhering fibers were mixed to bring the test composition into contact with the bacteria. . After 10 minutes, 1 ml of the contact solution was taken out and added to 9 ml drug-inactivated phosphate buffer, and the same operation was performed to dilute stepwise, followed by pour-culture on a standard agar medium (manufactured by Eiken Chemical Co., Ltd.) The number of viable bacteria was measured. And the difference of the common logarithm of the number of contact bacteria and the number of living bacteria calculated | required by the following formula (1) was made into the disinfection activity value, and it evaluated by the following four evaluation criteria.

[Equation 1]
Bactericidal activity value = Log (number of contacted bacteria-number of surviving bacteria) (1)

(Evaluation criteria)
(Double-circle): It is excellent in sterilization power (The sterilization activity value is 2 or more).
○: Excellent sterilizing power (bactericidal activity value is 1 or more and less than 2).
Δ: Slightly inferior in sterilizing power (bactericidal activity value of 0.5 or more and less than 1)
X: Inferior in sterilizing power (disinfecting activity value is less than 0.5).
Note that ◎ and ○ are practical.

[Detergency test]
(Test method)
Detergency was evaluated by the following method using a test protein-containing contaminated cloth (EMPA111, manufactured by Testfabrics) as a model of excrement dirt. That is, first, 0.15 g of each test composition was diluted in tap water (detergent usage concentration 0.15% by mass). And after putting EMPA111 in the said dilution liquid and performing washing | cleaning for 10 minutes at 25 degreeC using the washing | cleaning apparatus (Terg-O-Tomete, Daiei Kagaku Seiki Seisakusho Co., Ltd.), it calculates | requires by following formula (2). Based on the cleaning rate obtained, the evaluation was made according to the following four evaluation criteria.

[Equation 2]
Cleaning rate (%) = [(A−B) / (C−B)] × 100 (2)
A: Reflectance after washing the contaminated cloth, B: Reflectance before washing the contaminated cloth, C: Reflectance of the white cloth

(Evaluation criteria)
A: Excellent cleaning power (cleaning rate of 90% or more).
○: Excellent detergency (cleaning rate is 70% or more and less than 90%).
Δ: Slightly inferior in cleaning power (cleaning rate is 60% or more and less than 70%).
X: Inferior cleaning power (cleaning rate is less than 60%).
Note that ◎ and ○ are practical.

[Recontamination prevention test]
(Test method)
As a model of excrement stains, a protein-containing contaminated cloth for testing (EMPA111, manufactured by Testfabrics) was used and washed with a white cloth made of 100% cotton broad as in the above test method. The degree of recontamination was evaluated. That is, first, as a pretreatment, a detergent that does not contain a fluorescent agent is dissolved in hot water (40 to 50 ° C.) by a standard amount, and after washing the white cloth three times with this cleaning solution, the foam is sufficiently removed. Rinse. Then, after diluting 0.15 g of each test composition in tap water (detergent use concentration 0.15% by mass), EMPA111 and white cloth are put into each diluted solution, and a cleaning device (Terg-O-Tometer, Daiei Kagaku Seiki). Was washed at 25 ° C. for 10 minutes, and the degree of re-contamination on the white cloth was visually evaluated and evaluated according to the following four-stage evaluation criteria.

(Evaluation criteria)
A: There is no recontamination of dirt.
○: There is almost no recontamination of dirt.
Δ: There is recontamination of dirt.
X: Recontamination of dirt is remarkable.
Note that ◎, ○, and Δ are practical.

  From the above results, it can be seen that all of the products according to the examples have generally good evaluations and are highly practical. On the other hand, all of the comparative example products include evaluations with no practicality, and it is understood that there is a problem in practicality.

  INDUSTRIAL APPLICABILITY The present invention can be used to provide a liquid sterilizing detergent composition for washing that can inactivate non-enveloped viruses and remove bacteria, and that is also excellent in cleaning properties and re-contamination prevention properties.

Claims (5)

When the fiber product to which the non-enveloped virus is attached is washed in a washing machine in order to sterilize, wash and inactivate the non-enveloped virus, it is diluted to a concentration range of 0.1 to 0.5% by mass. A liquid sterilizing detergent composition for washing used in
The following components (A) to (C) are contained in the following proportions, the pH at 25 ° C. of a 0.1% by mass aqueous solution is in the range of 10.5 to 11.5, and inactivation against non-enveloped viruses: A liquid sterilizing detergent composition for washing, characterized by having properties.
(A) Quaternary ammonium salt 1 to 10 wt% a A Le kill dimethyl benzyl ammonium chloride Rye de.
(B) Alkali agent which is at least one selected from the group consisting of alkali hydroxide, carbonate, silicate and alkanolamine, and contains at least one of alkali hydroxide, carbonate and silicate. mass%.
(C) Citric acid, gluconic acid, succinic acid, tripolyphosphoric acid, hydroxyethylethylenediaminetriacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, nitrilotriacetic acid, triethylenetetraminehexaacetic acid, phosphonic acids, L-glutamic acid diacetic acid, methylglycine diacid 3 to 10 % by mass of a chelating agent which is at least one selected from the group consisting of acetic acid and alkali metal salts thereof .   The liquid sterilizing detergent composition for washing according to claim 1, comprising 1 to 30% by mass of a nonionic surfactant as the component (D) together with the components (A) to (C). The liquid sterilizing detergent composition for washing according to claim 1 or 2 , wherein the non-enveloped virus is a caliciviridae virus. The liquid sterilizing detergent composition for washing according to claim 3 , wherein the caliciviridae virus is a Norovirus virus. A non-enveloped virus, wherein the liquid sterilizing detergent composition for washing according to any one of claims 1 to 4 is diluted to a concentration range of 0.1 to 0.5 mass% and used as a washing liquid. A method for inactivating a non-enveloped virus in a fiber product, wherein the fabric product is washed with a washing machine to sterilize and wash the fiber product and inactivate a non-enveloped virus.