JP7069492B2 - 類似間葉系幹細胞の製造方法及びこれにより製造された類似間葉系幹細胞 - Google Patents
類似間葉系幹細胞の製造方法及びこれにより製造された類似間葉系幹細胞 Download PDFInfo
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Description
Claims (9)
- 類似間葉系幹細胞の製造方法であって、
(a)細胞株の確立後、70回以下の継代培養されたヒト多能性幹細胞を準備するステップと、
(b)前記ヒト多能性幹細胞を分化誘導した胚様体のうち、包嚢胚様体を選別するステップと、
(c)前記包嚢胚様体を細胞透過性3次元培養挿入体上にロードして類似間葉系幹細胞を分離するステップで、EGM(商標)-2MVである培養用培地を利用するステップと、
(d)前記細胞透過性3次元培養挿入体を透過した細胞のうち、単層(monolayer)形態の細胞群集のみを分離するステップと、
(e)前記単層形態の細胞群集を縦と横それぞれ100μm~500μmの大きさに均一化し、類似間葉系幹細胞を培養するステップと、
を含み、
前記類似間葉系幹細胞は、抗炎症効能及び免疫抑制能を有し、CD90及びSOX2を95%以上発現する、類似間葉系幹細胞の製造方法。 - 前記細胞透過性3次元培養挿入体は、ナイロン、繊維、ポリエチレン、ポリプロピレン、グラフェン、チタン、銅、ニッケル、銀、金、白金のいずれか1つ以上からなる、請求項1に記載の類似間葉系幹細胞の製造方法。
- 前記類似間葉系幹細胞は、骨髄由来間葉系幹細胞に比べてMMP-1タンパク質及びHGFタンパク質を高発現し、骨髄由来間葉系幹細胞に比べてCD95を低発現し、CD90及びSOX2を95%以上発現することを特徴とする、請求項1に記載の類似間葉系幹細胞の製造方法。
- 前記類似間葉系幹細胞は、骨髄由来間葉系幹細胞に比べてMMP-1タンパク質を15倍以上高発現し、HGFタンパク質を2倍以上高発現する、請求項1に記載の類似間葉系幹細胞の製造方法。
- 前記類似間葉系幹細胞は、骨髄由来間葉系幹細胞に比べてCD95を15倍以上低発現する、請求項1に記載の類似間葉系幹細胞の製造方法。
- 前記類似間葉系幹細胞は、骨髄由来間葉系幹細胞に比べて炎症調節遺伝子を2倍~100倍以上高発現し、
前記炎症調節遺伝子は、Gata3、Adora2a、Gps2、Psma1、Pbk、Lrfn5、Cdh5、Apoe、Foxf1、Tek、Cx3cl1、Ptger4、Acp5、Bcr、Socs5、及びMdkからなる群のいずれか1つ以上である、請求項1に記載の類似間葉系幹細胞の製造方法。 - 前記類似間葉系幹細胞は、Gata3、Adora2a、及びGps2遺伝子を同時に発現する、請求項1に記載の類似間葉系幹細胞の製造方法。
- 前記類似間葉系幹細胞は、骨髄由来間葉系幹細胞に比べて免疫抑制能遺伝子を2倍~100倍以上高発現し、
前記免疫抑制能遺伝子は、Gata3、Gps2、Psma1、Apoe、Foxf1、Tek、Cx3cl1、Ptger4、Bcr、Socs5及びMdkのいずれか1つ以上である、請求項1に記載の類似間葉系幹細胞の製造方法。 - 前記類似間葉系幹細胞は、Gata3、Gps2及びPsma1遺伝子を同時に発現する、請求項1に記載の類似間葉系幹細胞の製造方法。
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WO2011111787A1 (ja) | 2010-03-10 | 2011-09-15 | 株式会社ツーセル | 間葉系幹細胞を含む細胞製剤及びその製造方法 |
JP2015532596A (ja) | 2012-09-03 | 2015-11-12 | メディポスト・カンパニー・リミテッドMedipost Co., Ltd. | 間葉系幹細胞を培養するための方法 |
WO2019199234A1 (en) | 2018-04-12 | 2019-10-17 | Cellresearch Corporation Pte. Ltd. | A method of inducing or improving wound healing properties of mesenchymal stem cells |
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