JP6971219B2 - 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 - Google Patents

横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 Download PDF

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JP6971219B2
JP6971219B2 JP2018502120A JP2018502120A JP6971219B2 JP 6971219 B2 JP6971219 B2 JP 6971219B2 JP 2018502120 A JP2018502120 A JP 2018502120A JP 2018502120 A JP2018502120 A JP 2018502120A JP 6971219 B2 JP6971219 B2 JP 6971219B2
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エム.ビーチェム ジョーセフ
ミルズ ゴードン
ウォーレン チャールズ
メリット クリス
チョン チェミョン
エル.ダナウェイ ドウェイン
クラウダー スコット
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University of Texas System
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JP2018502120A 2015-07-17 2016-07-15 横断組織切片のユーザー定義領域における複数のタンパク質の同時定量 Active JP6971219B2 (ja)

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US201562193819P 2015-07-17 2015-07-17
US62/193,819 2015-07-17
US201562261654P 2015-12-01 2015-12-01
US62/261,654 2015-12-01
US201662277283P 2016-01-11 2016-01-11
US62/277,283 2016-01-11
US201662323018P 2016-04-15 2016-04-15
US62/323,018 2016-04-15
PCT/US2016/042455 WO2017015097A1 (en) 2015-07-17 2016-07-15 Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue

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Families Citing this family (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HRP20151294T1 (hr) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
CA2850509C (en) 2011-10-14 2023-08-01 President And Fellows Of Harvard College Sequencing by structure assembly
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP2971184B1 (en) 2013-03-12 2019-04-17 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
WO2014210223A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
ES2955488T3 (es) 2015-04-10 2023-12-01 Spatial Transcriptomics Ab Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
AU2016295158B2 (en) 2015-07-17 2021-02-25 Bruker Spatial Biology, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
SG10202107055SA (en) 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
CA3022290A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US11352667B2 (en) 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
US11597965B2 (en) 2017-10-06 2023-03-07 10X Genomics, Inc. RNA templated ligation
SG11202007501SA (en) 2018-02-12 2020-09-29 Nanostring Technologies Inc Biomolecular probes and methods of detecting gene and protein expression
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
EP3891299A1 (en) * 2018-12-04 2021-10-13 F. Hoffmann-La Roche AG Spatially oriented quantum barcoding of cellular targets
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
JP2022514892A (ja) 2018-12-21 2022-02-16 ナノストリング テクノロジーズ,インコーポレイティド 病理組織標本のモバイルデジタル空間プロファイリングのための方法、装置、システムおよびデバイス。
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN113906147B (zh) 2019-05-31 2024-09-13 10X基因组学有限公司 检测目标核酸分子的方法
US12362040B2 (en) 2019-10-18 2025-07-15 Bruker Spatial Biology, Inc. Systems and methods for spatial mapping of expression profiling
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
US12241890B2 (en) 2019-12-23 2025-03-04 10X Genomics, Inc. Methods for generating barcoded nucleic acid molecules using fixed cells
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
US20230235384A1 (en) * 2020-06-18 2023-07-27 Nanostring Technologies, Inc. Compositions and methods for in situ single cell analysis using enzymatic nucleic acid extension
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4341847A1 (en) * 2021-05-19 2024-03-27 Leica Microsystems CMS GmbH Marker, method and device for analyzing a biological sample
JP2024521682A (ja) * 2021-05-19 2024-06-04 ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング 生体試料または化合物または化学元素を分析するための方法
EP4092414B1 (en) * 2021-05-19 2025-01-29 Leica Microsystems CMS GmbH Connector, marker and method for analysing biological samples
EP4341848A1 (en) * 2021-05-19 2024-03-27 Leica Microsystems CMS GmbH Marker, method and device for analyzing a biological sample
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
WO2023009842A1 (en) 2021-07-30 2023-02-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US12497653B2 (en) 2022-01-21 2025-12-16 10X Genomics, Inc. Multiple readout signals for analyzing a sample
EP4220162A3 (en) * 2022-02-01 2023-10-18 Leica Microsystems CMS GmbH Connector, connector-label conjugate and marker for analysing biological samples
EP4487159A1 (en) * 2022-03-01 2025-01-08 University of Washington Apparatuses systems and methods for three dimensional microdissection of samples
WO2023172915A1 (en) 2022-03-08 2023-09-14 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
EP4499867A2 (en) 2022-03-24 2025-02-05 Digital Biology Inc. Tissue spatial omics
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
JP2025516589A (ja) 2022-05-11 2025-05-30 10エックス ジェノミクス インコーポレイテッド インサイチュー配列決定のための組成物及び方法
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
AU2023308992A1 (en) 2022-07-19 2025-01-30 Digital Biology Inc. Barcode diffusion-based spatial omics
EP4511510B1 (en) 2022-08-16 2025-12-10 10X Genomics, Inc. Ap50 polymerases and uses thereof
WO2024041749A1 (en) * 2022-08-23 2024-02-29 Leica Microsystems Cms Gmbh Sequencing scaffold and method
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification

Family Cites Families (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
US5543838A (en) 1993-08-31 1996-08-06 Xerox Corporation Signal multiplexing system for an image sensor array
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
WO1997007245A1 (en) 1995-08-14 1997-02-27 Ely Michael Rabani Methods and devices for parallel multiplex polynucleotide sequencing
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6387707B1 (en) 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US7144119B2 (en) 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
EP0963443B1 (en) 1996-12-10 2006-03-08 Sequenom, Inc. Releasable nonvolatile mass-label molecules
WO1998030721A1 (en) 1997-01-10 1998-07-16 Pioneer Hi-Bred International, Inc. Hybridization-based genetic amplification and analysis
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
DE69836734D1 (de) 1997-02-20 2007-02-08 Univ California Plasmon-schwingteilchen, methode und vorrichtung
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
JP4357112B2 (ja) 1997-10-14 2009-11-04 ルミネックス コーポレイション 精密蛍光染色された粒子及びその製造及び使用方法
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
JP3662850B2 (ja) 1998-06-24 2005-06-22 イルミナ インコーポレイテッド 微小球を有するアレイセンサーのデコード
GB2342651B (en) 1998-09-18 2001-10-17 Massachusetts Inst Technology Biological applications of semiconductor nanocrystals
US7079241B2 (en) 2000-04-06 2006-07-18 Invitrogen Corp. Spatial positioning of spectrally labeled beads
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
DE60042738D1 (de) 1999-05-07 2009-09-24 Life Technologies Corp Verfahren zum nachweis von analyten mit hilfe von halbleiternanokrystallen
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2001000875A2 (en) 1999-06-25 2001-01-04 Motorola, Inc. Novel methods and products for arrayed microsphere analysis
US7214477B1 (en) 1999-07-26 2007-05-08 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Layered device with capture regions for cellular analysis
AU771499B2 (en) 1999-07-26 2004-03-25 Government of The United States of America, as represented by The Secretary Department of Health & Human Services, The National Institutes of Health, The Layered device with capture regions for cellular analysis
EP1212599A2 (en) 1999-08-30 2002-06-12 Illumina, Inc. Methods for improving signal detection from an array
US7585632B2 (en) 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
WO2001033190A2 (en) 1999-11-04 2001-05-10 Arcturus Engineering, Inc. Automated laser capture microdissection
US20020039728A1 (en) 2000-02-10 2002-04-04 Robert Kain Alternative substrates and formats for bead-based array of arrays
US20020028457A1 (en) 2000-02-16 2002-03-07 Quantum Dot Corporation Single target counting assays using semiconductor nanocrystals
CA2399189A1 (en) 2000-02-22 2001-08-30 Genospectra, Inc. Microarray fabrication techniques and apparatus
US20030186426A1 (en) 2000-03-15 2003-10-02 The Regents Of The University Of California Multichannel flow cell for interacting single optically trapped, DNA molecules with different chemical species
AU2001278133A1 (en) 2000-08-01 2002-02-13 Surromed, Inc. Methods for solid phase nanoextraction and desorption
US6777244B2 (en) 2000-12-06 2004-08-17 Hrl Laboratories, Llc Compact sensor using microcavity structures
EP2465943A3 (en) 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
JP2005505749A (ja) 2001-05-21 2005-02-24 アクララ バイオサイエンシーズ, インコーポレイテッド タンパク質を分析するための方法および組成物
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
CA2492007A1 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Amplification of nucleic acid fragments using nicking agents
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
US20040229294A1 (en) 2002-05-21 2004-11-18 Po-Ying Chan-Hui ErbB surface receptor complexes as biomarkers
US20040000519A1 (en) 2002-06-28 2004-01-01 Postnova Analytics, Inc. Field-flow fractionation method and apparatus
US20100075858A1 (en) 2003-04-29 2010-03-25 Genvault Corporation Biological bar code
US20050048498A1 (en) 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
JPWO2005027747A1 (ja) 2003-09-24 2006-11-24 松下電器産業株式会社 超音波診断装置
WO2005037071A2 (en) 2003-10-14 2005-04-28 Monogram Biosciences, Inc. Receptor tyrosine kinase signaling pathway analysis for diagnosis and therapy
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US7569392B2 (en) 2004-01-08 2009-08-04 Vanderbilt University Multiplex spatial profiling of gene expression
US7295026B2 (en) * 2005-06-03 2007-11-13 Ut-Battelle, Llc Automated position control of a surface array relative to a liquid microjunction surface sampler
EP2322921B1 (en) * 2005-06-07 2014-08-06 Centre National de la Recherche Scientifique (CNRS) DNTP conjugated to peptide via a UV cleavable linker
CN101495650B (zh) 2005-06-20 2015-02-04 领先细胞医疗诊断有限公司 检测单个细胞中的核酸和鉴定异质大细胞群中罕见细胞的方法
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
WO2007076128A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
US8986926B2 (en) 2005-12-23 2015-03-24 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
EP2030011B1 (en) 2006-05-22 2017-02-01 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
US8797644B2 (en) * 2006-08-11 2014-08-05 The Regents Of The University Of California Capillary-based cell and tissue acquisition system (CTAS)
US7741045B2 (en) 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
US7728287B2 (en) 2007-03-01 2010-06-01 Lawrence Livermore National Security, Llc Imaging mass spectrometer with mass tags
AU2008237018B2 (en) * 2007-04-10 2014-04-03 Bruker Spatial Biology, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
GB0811574D0 (en) 2008-06-24 2008-07-30 Trillion Genomics Ltd Characterising planar samples by mass spectrometry
AU2009274115A1 (en) 2008-07-21 2010-01-28 Neodiagnostix, Inc. Methods for the cytological analysis of cervical cells
WO2010019826A1 (en) 2008-08-14 2010-02-18 Nanostring Technologies, Inc Stable nanoreporters
EP2163900A1 (en) 2008-09-04 2010-03-17 Commissariat A L'energie Atomique New method of imaging by mass spectrometry and new mass tag associated trityl derivatives
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
DE102008062372B3 (de) 2008-12-17 2010-06-17 Medizinische Hochschule Hannover Nachweiskonjugat und Verfahren zur Analyse
US20110275077A1 (en) 2009-01-12 2011-11-10 William James Oligonucleotide-Coated Affinity Membranes and Uses Thereof
SG10201408392PA (en) 2009-01-15 2015-01-29 Lab Corp America Holdings Methods of determining patient response by measurement of her-3
US8753824B2 (en) 2009-04-03 2014-06-17 University Of Virginia Patent Foundation Serial multiple antigen colocalization in paraffin-embedded tissue
WO2011038403A1 (en) * 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
HRP20151294T1 (hr) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US10144950B2 (en) 2011-01-31 2018-12-04 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CA2836577C (en) 2011-05-17 2019-06-25 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
JP2015507928A (ja) 2012-02-14 2015-03-16 ザ・ジョンズ・ホプキンス・ユニバーシティ Mirna解析方法
US10174310B2 (en) 2012-08-08 2019-01-08 Roche Sequencing Solutions, Inc. Increasing dynamic range for identifying multiple epitopes in cells
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
EP2909337B1 (en) 2012-10-17 2019-01-09 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
HK1214650A1 (zh) 2012-10-26 2016-07-29 Fluidigm Canada Inc. 通过质谱流式细胞术的样品分析
JP6419703B2 (ja) 2012-10-26 2018-11-07 フリューダイム カナダ インコーポレイテッド マスサイトメトリーによる細胞分析
KR101969847B1 (ko) 2012-10-30 2019-04-17 삼성전자주식회사 동일 검체에서 여러 항원을 검출하기 위한 순차적 다중 면역염색법
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
WO2014182528A2 (en) 2013-04-30 2014-11-13 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding
EP3008201B1 (en) 2013-06-12 2019-08-07 The General Hospital Corporation Methods for multiplexed detection of target molecules and uses thereof
HK1223659A1 (zh) 2013-06-14 2017-08-04 Nanostring Technologies, Inc. 基於可多重标签的报告系统
AU2014318698B2 (en) 2013-09-13 2019-10-24 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
US9322051B2 (en) 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
KR102538555B1 (ko) * 2014-01-29 2023-05-30 케이엠 바이올로직스 가부시키가이샤 항-트랜스티레틴 인간화 항체
EP3126512B1 (en) 2014-02-26 2019-04-17 Ventana Medical Systems, Inc. Photo-selective method for biological sample analysis field
US9312111B2 (en) 2014-04-02 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for sub-micrometer elemental image analysis by mass spectrometry
MX2016013156A (es) 2014-04-10 2017-02-14 10X Genomics Inc Dispositivos, sistemas y metodos fluidicos, para encapsular y dividir reactivos, y aplicaciones de los mismos.
EP3137100B1 (en) * 2014-04-15 2023-12-20 University Of Virginia Patent Foundation Isolated t cell receptors and methods of use therefor
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
EP3223865A4 (en) * 2014-10-31 2018-10-03 Jounce Therapeutics, Inc. Methods of treating conditions with antibodies that bind b7-h4
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN113403373B (zh) 2014-11-21 2025-04-11 布鲁克空间生物学公司 无酶且无扩增的测序
ES2955488T3 (es) 2015-04-10 2023-12-01 Spatial Transcriptomics Ab Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
SG10202107055SA (en) 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
AU2016295158B2 (en) 2015-07-17 2021-02-25 Bruker Spatial Biology, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
CA3242290A1 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
US10357770B2 (en) * 2015-10-09 2019-07-23 International Business Machines Corporation Microfluidic probe for modulating insertion of liquid spacers
CA3032649A1 (en) 2016-08-01 2018-02-08 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
EP3541957B1 (en) 2016-11-21 2025-06-25 Bruker Spatial Biology, Inc. A method for sequencing nucleic acids
SG11202007501SA (en) 2018-02-12 2020-09-29 Nanostring Technologies Inc Biomolecular probes and methods of detecting gene and protein expression
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN

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