JP6952706B2 - エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 - Google Patents

エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 Download PDF

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JP6952706B2
JP6952706B2 JP2018546669A JP2018546669A JP6952706B2 JP 6952706 B2 JP6952706 B2 JP 6952706B2 JP 2018546669 A JP2018546669 A JP 2018546669A JP 2018546669 A JP2018546669 A JP 2018546669A JP 6952706 B2 JP6952706 B2 JP 6952706B2
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antibody
epitope
epitope tag
seq
tag
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JP2019512224A (ja
JP2019512224A5 (cg-RX-API-DMAC7.html
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マイケル ファレル，
マイケル ファレル，
エリック メイ，
エリック メイ，
レイ タン，
レイ タン，
ウェンチュン チャン，
ウェンチュン チャン，
アントニー ハバード，
アントニー ハバード，
ツー−シュエン ツァオ，
ツー−シュエン ツァオ，
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ヴェンタナ メディカル システムズ， インク．
ヴェンタナ メディカル システムズ， インク．
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018546669A 2016-03-08 2017-03-07 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学 Active JP6952706B2 (ja)

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Application Number Priority Date Filing Date Title
JP2021156935A JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2022177332A JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662305440P 2016-03-08 2016-03-08
US62/305,440 2016-03-08
US201662418667P 2016-11-07 2016-11-07
US62/418,667 2016-11-07
US201762461651P 2017-02-21 2017-02-21
US62/461,651 2017-02-21
PCT/US2017/021157 WO2017155996A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

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JP2021156935A Division JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

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JP2019512224A JP2019512224A (ja) 2019-05-16
JP2019512224A5 JP2019512224A5 (cg-RX-API-DMAC7.html) 2020-04-16
JP6952706B2 true JP6952706B2 (ja) 2021-10-20

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JP2021156935A Active JP7174126B2 (ja) 2016-03-08 2021-09-27 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学
JP2022177332A Pending JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

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JP2022177332A Pending JP2023024990A (ja) 2016-03-08 2022-11-04 エピトープタグを有する組換え抗体を使用する多重化免疫組織化学

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US (3) US11112413B2 (cg-RX-API-DMAC7.html)
EP (1) EP3426684B1 (cg-RX-API-DMAC7.html)
JP (3) JP6952706B2 (cg-RX-API-DMAC7.html)
AU (1) AU2017229370B2 (cg-RX-API-DMAC7.html)
CA (1) CA3016555A1 (cg-RX-API-DMAC7.html)
WO (1) WO2017155996A1 (cg-RX-API-DMAC7.html)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA43135A (fr) 2015-10-30 2018-09-05 Aleta Biotherapeutics Inc Compositions et méthodes pour le du traitement du cancer
BR112018008390A2 (pt) 2015-10-30 2018-11-27 Aleta Biotherapeutics Inc composições e métodos para transdução tumoral
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
US20210188953A1 (en) * 2017-11-07 2021-06-24 Trustees Of Tufts College Heteromultimeric binding molecules for toxin neutralization, compositions and methods thereof
WO2020206175A1 (en) * 2019-04-03 2020-10-08 Access Medical Systems, Ltd. Method for re-using hapten-coated probe in an immunoassay
CN110850094A (zh) * 2019-11-22 2020-02-28 西安交通大学 一种免疫组化双标单染试剂盒及其使用方法和应用
CN114113616B (zh) * 2021-12-07 2022-08-23 深圳裕策生物科技有限公司 一种试剂盒及其染色方法
JP7783531B2 (ja) 2022-02-22 2025-12-10 Ｎｔｔ株式会社 センサー付きハイドロゲル流路型デバイス
EP4590709A1 (en) 2022-09-22 2025-07-30 Agilent Technologies, Inc. Anti-human icos antibodies for use in immunohistochemistry (ihc) protocols and for diagnosing cancer

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1564604A (en) 1925-06-12 1925-12-08 Chas S Hodges Water wheel
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
CA2320750C (en) 1998-02-27 2004-08-24 Ventana Medical Systems, Inc. System and method of aspirating and dispensing reagent
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
JP4648902B2 (ja) 2003-06-24 2011-03-09 ベンタナ・メデイカル・システムズ・インコーポレーテツド 免疫組織化学的エピトープおよび核酸配列のインサイチュ検出が向上するように酵素の触媒作用で金属を付着
US20060246523A1 (en) 2005-04-28 2006-11-02 Christopher Bieniarz Antibody conjugates
WO2007062177A2 (en) 2005-11-23 2007-05-31 Ventana Medical Systems, Inc. Molecular conjugate
CA3069091C (en) 2006-11-01 2021-09-14 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
EP2167963B1 (en) 2007-05-23 2019-04-17 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US20080299555A1 (en) * 2007-05-30 2008-12-04 Hiroaki Nitta Multicolor chromogenic detection of biomarkers
WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
AU2009282760B2 (en) 2008-08-22 2013-08-15 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
AU2009311233B2 (en) * 2008-11-06 2015-01-15 University Of Guelph Methods of improving the therapeutic efficacy and utility of antibody fragments
KR101424414B1 (ko) 2009-10-19 2014-08-01 벤타나 메디컬 시스템즈, 인코포레이티드 현미경 스테이지에 대한 디바이스
WO2011139978A1 (en) 2010-05-04 2011-11-10 Ventana Medical Systems, Inc. Moving meniscus rinsing and mixing in cell staining
EP2588443A2 (en) 2010-07-02 2013-05-08 Ventana Medical Systems, Inc. Hapten conjugates for target detection
CN103097412B (zh) * 2010-07-09 2016-08-10 克鲁塞尔荷兰公司 抗人呼吸道合胞病毒(rsv)抗体以及使用方法
JP6644713B2 (ja) 2014-02-24 2020-02-12 ヴェンタナ メディカル システムズ， インク． キノンメチドアナログシグナル増幅
JP7095995B2 (ja) 2015-04-20 2022-07-05 ベンタナ メディカル システムズ， インコーポレイテッド 組織学的試料のための試薬のインクジェット沈着
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

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JP2019512224A (ja) 2019-05-16
US11988673B2 (en) 2024-05-21
WO2017155996A1 (en) 2017-09-14
JP2023024990A (ja) 2023-02-21
JP7174126B2 (ja) 2022-11-17
AU2017229370B2 (en) 2021-03-11
EP3426684A1 (en) 2019-01-16
US11112413B2 (en) 2021-09-07
AU2017229370A1 (en) 2018-08-16
EP3426684B1 (en) 2024-10-09
US20190094242A1 (en) 2019-03-28
US20220057408A1 (en) 2022-02-24
JP2022003064A (ja) 2022-01-11
US20240230667A1 (en) 2024-07-11
CA3016555A1 (en) 2017-09-14

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