JP6934477B2 - 熱安定逆転写酵素 - Google Patents

熱安定逆転写酵素 Download PDF

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JP6934477B2
JP6934477B2 JP2018538718A JP2018538718A JP6934477B2 JP 6934477 B2 JP6934477 B2 JP 6934477B2 JP 2018538718 A JP2018538718 A JP 2018538718A JP 2018538718 A JP2018538718 A JP 2018538718A JP 6934477 B2 JP6934477 B2 JP 6934477B2
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residue
polymerase
amino acid
rna
dna
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JP2019509717A (ja
JP2019509717A5 (https=
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アンドリュー エリントン
アンドリュー エリントン
ジャレッド エレフソン
ジャレッド エレフソン
ジミー ディー ゴリハー
ジミー ディー ゴリハー
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ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム
ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2018538718A 2016-01-19 2017-01-19 熱安定逆転写酵素 Active JP6934477B2 (ja)

Priority Applications (1)

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JP2021135313A JP7256849B2 (ja) 2016-01-19 2021-08-23 熱安定逆転写酵素

Applications Claiming Priority (3)

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US201662280451P 2016-01-19 2016-01-19
US62/280,451 2016-01-19
PCT/US2017/014082 WO2017127510A2 (en) 2016-01-19 2017-01-19 Thermostable reverse transcriptase

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JP2021135313A Division JP7256849B2 (ja) 2016-01-19 2021-08-23 熱安定逆転写酵素

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JP2019509717A JP2019509717A (ja) 2019-04-11
JP2019509717A5 JP2019509717A5 (https=) 2020-02-27
JP6934477B2 true JP6934477B2 (ja) 2021-09-15

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US (3) US10323243B2 (https=)
EP (2) EP3405578B1 (https=)
JP (2) JP6934477B2 (https=)
WO (1) WO2017127510A2 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6934477B2 (ja) 2016-01-19 2021-09-15 ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム 熱安定逆転写酵素
EP3720606A4 (en) * 2017-07-27 2021-05-05 Board Of Regents, The University Of Texas System AMPLIFICATION OF PAIRED PROTEINCODING MRNA SEQUENCES
WO2019084538A1 (en) * 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
JP7593316B2 (ja) * 2019-07-26 2024-12-03 東洋紡株式会社 安定性に優れた変異型逆転写酵素
WO2024238992A1 (en) * 2023-05-18 2024-11-21 10X Genomics, Inc. Engineered non-strand displacing family b polymerases for reverse transcription and gap-fill applications
US12467041B2 (en) 2023-12-12 2025-11-11 Hubei University Pfu DNA polymerase mutants with reverse transcriptase activity and their applications
CN117778348B (zh) * 2023-12-12 2024-09-20 湖北大学 一种具有逆转录酶活性的Pfu DNA聚合酶突变体及其应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US20030228616A1 (en) 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
DE60141097D1 (de) * 2000-05-11 2010-03-11 Toyo Boseki Modifizierte thermostabile dna polymerase von pyrococcus kodakaraensis
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US20030134292A1 (en) * 2001-10-30 2003-07-17 Farchaus Joseph W. Thermostable DNA polymerases and methods of making same
ATE427992T1 (de) * 2001-11-14 2009-04-15 Toyo Boseki Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
US7932070B2 (en) * 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
EP1801213A3 (en) 2003-11-03 2007-10-24 Medical Research Council Polymerase
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
ES2404665T3 (es) 2006-09-06 2013-05-28 Medical Research Council Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante
US20090305345A1 (en) 2006-10-23 2009-12-10 Medical Research Council Polymerase
CA2742593C (en) * 2008-11-03 2020-05-05 Kapa Biosystems, Inc. Chimeric dna polymerases
US20110301041A1 (en) * 2010-02-26 2011-12-08 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
GB201007384D0 (en) * 2010-04-30 2010-06-16 Medical Res Council Enzymes
JP6934477B2 (ja) 2016-01-19 2021-09-15 ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム 熱安定逆転写酵素

Also Published As

Publication number Publication date
US11912991B2 (en) 2024-02-27
JP2019509717A (ja) 2019-04-11
WO2017127510A2 (en) 2017-07-27
US10858652B2 (en) 2020-12-08
US20210130816A1 (en) 2021-05-06
WO2017127510A3 (en) 2017-10-12
EP4067491A1 (en) 2022-10-05
JP7256849B2 (ja) 2023-04-12
US20170327818A1 (en) 2017-11-16
JP2021192618A (ja) 2021-12-23
EP3405578A2 (en) 2018-11-28
EP3405578A4 (en) 2019-09-25
EP3405578B1 (en) 2022-03-16
US20190376059A1 (en) 2019-12-12
US10323243B2 (en) 2019-06-18

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