JP6912887B2 - トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法 - Google Patents

トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法 Download PDF

Info

Publication number
JP6912887B2
JP6912887B2 JP2016538724A JP2016538724A JP6912887B2 JP 6912887 B2 JP6912887 B2 JP 6912887B2 JP 2016538724 A JP2016538724 A JP 2016538724A JP 2016538724 A JP2016538724 A JP 2016538724A JP 6912887 B2 JP6912887 B2 JP 6912887B2
Authority
JP
Japan
Prior art keywords
peptide
transfection
cells
lipid
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2016538724A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017500856A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html
JP2017500856A (ja
Inventor
モレラ デュ ジュ ザビエル ド
モレラ デュ ジュ ザビエル ド
Original Assignee
ライフ テクノロジーズ コーポレーション
ライフ テクノロジーズ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ライフ テクノロジーズ コーポレーション, ライフ テクノロジーズ コーポレーション filed Critical ライフ テクノロジーズ コーポレーション
Publication of JP2017500856A publication Critical patent/JP2017500856A/ja
Publication of JP2017500856A5 publication Critical patent/JP2017500856A5/ja
Priority to JP2019168228A priority Critical patent/JP6985347B2/ja
Application granted granted Critical
Publication of JP6912887B2 publication Critical patent/JP6912887B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
    • A61K47/544—Phospholipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
    • A61K47/6455—Polycationic oligopeptides, polypeptides or polyamino acids, e.g. for complexing nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
JP2016538724A 2013-12-12 2014-12-12 トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法 Active JP6912887B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2019168228A JP6985347B2 (ja) 2013-12-12 2019-09-17 トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361915429P 2013-12-12 2013-12-12
US61/915,429 2013-12-12
PCT/US2014/070176 WO2015089487A1 (en) 2013-12-12 2014-12-12 Membrane-penetrating peptides to enhance transfection and compositions and methods for using same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019168228A Division JP6985347B2 (ja) 2013-12-12 2019-09-17 トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法

Publications (3)

Publication Number Publication Date
JP2017500856A JP2017500856A (ja) 2017-01-12
JP2017500856A5 JP2017500856A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2017-07-27
JP6912887B2 true JP6912887B2 (ja) 2021-08-04

Family

ID=52273587

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2016538724A Active JP6912887B2 (ja) 2013-12-12 2014-12-12 トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法
JP2019168228A Active JP6985347B2 (ja) 2013-12-12 2019-09-17 トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019168228A Active JP6985347B2 (ja) 2013-12-12 2019-09-17 トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法

Country Status (11)

Country Link
US (5) US9856496B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (2) EP3079723B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (2) JP6912887B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (1) KR102532559B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (2) CN106456793B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (2) AU2014361806B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (1) BR112016013516A8 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA2933561A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES2808866T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
SG (2) SG11201604697TA (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2015089487A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3051089C (en) * 2012-05-02 2022-04-12 Life Technologies Corporation High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers
CN104583956B (zh) 2012-06-15 2019-01-04 英特尔公司 用于实现加载存储重新排序和优化的指令定义
CN104823168B (zh) 2012-06-15 2018-11-09 英特尔公司 用于实现从由加载存储重新排序和优化导致的推测性转发遗漏预测/错误中恢复的方法和系统
KR101996462B1 (ko) 2012-06-15 2019-07-04 인텔 코포레이션 명확화 없는 비순차 load store 큐
CN104583943B (zh) 2012-06-15 2018-06-08 英特尔公司 拥有具有分布式结构的动态分派窗口的虚拟加载存储队列
CN104823154B (zh) 2012-06-15 2017-12-29 英特尔公司 包括虚拟加载存储队列的处理器和系统
WO2013188306A1 (en) 2012-06-15 2013-12-19 Soft Machines, Inc. Reordered speculative instruction sequences with a disambiguation-free out of order load store queue
EP3079723B1 (en) 2013-12-12 2020-05-27 Life Technologies Corporation Membrane-penetrating peptides to enhance transfection and compositions and methods for using same
WO2016131052A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
US20170016043A1 (en) 2015-07-13 2017-01-19 Life Technologies Corporation System and method for improved transient protein expression in cho cells
CN108779074A (zh) * 2016-03-01 2018-11-09 分子传递有限公司 植物病毒移动蛋白和其使用方法
EP3500585A4 (en) 2016-08-17 2020-04-01 Factor Bioscience Inc. NUCLEIC ACID PRODUCTS AND METHODS OF ADMINISTRATION
US11414675B2 (en) * 2016-09-30 2022-08-16 Life Technologies Corporation Serum-free suspension system for lentiviral production
US11782050B2 (en) 2017-09-19 2023-10-10 Cannametrix, Llc Cell-based assay for quantifying the potency and efficacy of cannabinoids and/or terpenoids, and methods of use thereof
WO2019178479A1 (en) * 2018-03-16 2019-09-19 Sarepta Therapeutics, Inc. Chimeric peptides for antisense delivery
GB201821269D0 (en) 2018-12-28 2019-02-13 Nippon Shinyaku Co Ltd Myostatin signal inhibitor
US11608491B2 (en) 2019-02-22 2023-03-21 Life Technologies Corporation Suspension system for adeno associated virus production
EP4005583A4 (en) * 2019-04-01 2023-04-26 Industry - University Cooperation Foundation Hanyang University CP2C TARGETING PEPTIDE ANTICANCER AGENT
KR102261371B1 (ko) * 2019-04-01 2021-06-08 한양대학교 산학협력단 CP2c 표적 펩티드 기반 항암제
EP4010028A1 (en) * 2019-08-05 2022-06-15 PolyPlus Transfection Compositions for transfecting a nucleic acid molecule into a cell comprising triazole compounds grafted to a cationic polymer, and their applications
KR102515464B1 (ko) * 2020-04-29 2023-03-28 연세대학교 원주산학협력단 핵산 또는 유전자 형질감염 효율을 개선하는 세포독성 완화 막 투과 펩타이드 및 이의 용도
CA3207656A1 (en) * 2021-02-08 2022-08-11 University Of British Columbia Anti-synucleinopathy peptide and methods to treat neurodegenerative diseases
TW202317147A (zh) 2021-07-08 2023-05-01 日商日本新藥股份有限公司 析出抑制劑
JPWO2023282345A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2021-07-08 2023-01-12
CN118201606A (zh) 2021-07-08 2024-06-14 日本新药株式会社 肾毒性减轻剂
CN113563429A (zh) * 2021-07-19 2021-10-29 天津大学 一种基于烷基化多肽的核酸递送系统及制备方法与应用
WO2023014909A1 (en) * 2021-08-04 2023-02-09 AJK Biopharmaceutical, LLC Amphiphilic peptides for nucleic acid and protein delivery
CN114106095B (zh) * 2021-11-11 2023-06-09 华南理工大学 基于聚集诱导发光原理的细胞自噬检测分子探针及其制备方法和应用
WO2023171804A1 (ja) 2022-03-10 2023-09-14 日本新薬株式会社 抗ウイルスアンチセンスオリゴマー
KR102717793B1 (ko) * 2022-05-26 2024-10-16 주식회사 바이오셀트란 신규한 미백 펩타이드 및 이를 함유하는 화장료 조성물
EP4547282A1 (en) 2022-06-30 2025-05-07 Life Technologies Corporation Lipid compositions for in vivo delivery
GB202211597D0 (en) 2022-08-09 2022-09-21 Nchain Licensing Ag Computer-implemented method and system
CN120615011A (zh) 2022-12-29 2025-09-09 生命科技股份有限公司 脂质组合物及用于递送到免疫细胞的方法
CN115947817B (zh) * 2023-02-22 2024-07-02 甘肃中肽生物科技有限公司 一种肿瘤相关多肽及其制备方法和应用

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
FR2645866B1 (fr) 1989-04-17 1991-07-05 Centre Nat Rech Scient Nouvelles lipopolyamines, leur preparation et leur emploi
US5279833A (en) 1990-04-04 1994-01-18 Yale University Liposomal transfection of nucleic acids into animal cells
US5334761A (en) 1992-08-28 1994-08-02 Life Technologies, Inc. Cationic lipids
US5316948A (en) 1992-09-04 1994-05-31 Life Technologies, Inc. N4 -methyl-2'-deoxycytidine 5'-triphosphate and its use in polymerase-catalyzed nucleic acid syntheses
WO1995002698A1 (en) 1993-07-12 1995-01-26 Life Technologies, Inc. Composition and methods for transfecting eukaryotic cells
US5674908A (en) 1993-12-20 1997-10-07 Life Technologies, Inc. Highly packed polycationic ammonium, sulfonium and phosphonium lipids
US6075012A (en) 1994-02-11 2000-06-13 Life Technologies, Inc. Reagents for intracellular delivery of macromolecules
US5627159A (en) 1994-10-27 1997-05-06 Life Technologies, Inc. Enhancement of lipid cationic transfections in the presence of serum
US6051429A (en) * 1995-06-07 2000-04-18 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections
EP0874910A4 (en) 1995-06-07 1999-04-21 Life Technologies Inc ENHANCED CATIONIC LIPID TRANSFECTION BY PEPTIDES
US5744335A (en) 1995-09-19 1998-04-28 Mirus Corporation Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein
SE9804614A0 (en) 1998-07-06 2000-01-07 A+ Science Invest Ab New peptides and use thereof
DE69937964T2 (de) 1998-11-12 2009-01-02 Invitrogen Corp., Carlsbad Transportreagentien
US6664040B2 (en) 2000-05-23 2003-12-16 The Regents Of The University Of California Compositions and methods for delivery of a molecule into a cell
PT1301213T (pt) * 2000-07-21 2017-04-19 Revance Therapeutics Inc Sistemas de transporte biológico de múltiplos componentes
GB0103110D0 (en) 2000-08-25 2001-03-28 Aventis Pharma Inc A membrane penetrating peptide encoded by a nuclear localization sequence from human period 1
GB0106315D0 (en) * 2001-03-14 2001-05-02 Ich Productions Ltd Transfection complexes
AU2004204456A1 (en) 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
JP4846588B2 (ja) 2003-05-22 2011-12-28 モレキュラー、トランスファー、インコーポレイテッド 核酸のトランスフェクションのための新規脂質
DK1653989T3 (da) * 2003-08-14 2009-11-23 Cellectis Antibakteriel sammensætning især mod gramnegative bakterier og som omfatter et peptid og et fortrinsvist hydrofobt antibakterielt middel
US20050288246A1 (en) * 2004-05-24 2005-12-29 Iversen Patrick L Peptide conjugated, inosine-substituted antisense oligomer compound and method
CA2633063A1 (en) 2005-12-16 2007-06-21 Diatos Cell penetrating peptide conjugates for delivering of nucleic acids intocells
US20100061932A1 (en) 2005-12-30 2010-03-11 Evonik Roehm Gmbh Peptides useful as cell-penetrating peptides
JP5313867B2 (ja) 2006-03-30 2013-10-09 ドライス ファーマシューティカルズ，インコーポレイティド カンプトテシン−細胞透過性ペプチド複合体及びそれを含む医薬組成物
CA2960570C (en) 2006-05-05 2018-05-08 Molecular Transfer, Inc. Lipids for transfection of eukaryotic cells
JP5403681B2 (ja) 2007-03-07 2014-01-29 独立行政法人国立国際医療研究センター 新規核内移行ペプチド
AU2009329893A1 (en) * 2008-12-23 2011-06-30 Vivoscript, Inc. Compositions and methods for re-programming cells without genetic modification
US20110053829A1 (en) 2009-09-03 2011-03-03 Curevac Gmbh Disulfide-linked polyethyleneglycol/peptide conjugates for the transfection of nucleic acids
US9006415B2 (en) * 2010-04-06 2015-04-14 Massachusetts Institute Of Technology Targeted delivery of nucleic acids
CA2800741C (en) * 2010-05-30 2018-07-24 The Governing Council Of The University Of Toronto Mitochondrial penetrating peptides as carriers for anticancer compounds
NZ603732A (en) 2010-06-14 2015-02-27 Hoffmann La Roche Cell-penetrating peptides and uses therof
WO2012000104A1 (en) 2010-06-30 2012-01-05 Protiva Biotherapeutics, Inc. Non-liposomal systems for nucleic acid delivery
EP3470395A1 (en) 2010-11-15 2019-04-17 Life Technologies Corporation Amine-containing transfection reagents and methods for making and using same
CN102153629B (zh) * 2011-01-20 2013-07-24 华中科技大学 一种短肽及其应用
US8853145B2 (en) * 2011-11-14 2014-10-07 Carlos Witte-Hoffmann BLID; a novel protein domain for interaction with the Bcl-2 family of proteins
US20150266939A1 (en) 2012-03-15 2015-09-24 Permeon Biologics, Inc. Cell penetrating compositions for delivery of intracellular antibodies and antibody-like moieties and methods of use
EP3079723B1 (en) 2013-12-12 2020-05-27 Life Technologies Corporation Membrane-penetrating peptides to enhance transfection and compositions and methods for using same
KR102476764B1 (ko) * 2015-12-23 2022-12-14 에스케이하이닉스 주식회사 소자분리구조 및 그 제조 방법
US9926825B2 (en) 2016-04-19 2018-03-27 GM Global Technology Operations LLC Method and apparatus for exhaust purification for an internal combustion engine

Also Published As

Publication number Publication date
EP3756690A3 (en) 2021-03-17
US20200392537A1 (en) 2020-12-17
SG11201604697TA (en) 2016-07-28
AU2014361806B2 (en) 2019-12-19
AU2020202002A1 (en) 2020-04-09
CN113105559A (zh) 2021-07-13
WO2015089487A1 (en) 2015-06-18
US12152248B2 (en) 2024-11-26
US9856496B2 (en) 2018-01-02
EP3079723A1 (en) 2016-10-19
KR102532559B1 (ko) 2023-05-16
US10760098B2 (en) 2020-09-01
US20150211021A1 (en) 2015-07-30
ES2808866T3 (es) 2021-03-02
AU2014361806A1 (en) 2016-07-28
SG10201811729PA (en) 2019-02-27
JP6985347B2 (ja) 2021-12-22
BR112016013516A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2017-08-08
US20250109412A1 (en) 2025-04-03
EP3756690A2 (en) 2020-12-30
EP3079723B1 (en) 2020-05-27
US20180163231A1 (en) 2018-06-14
CN106456793A (zh) 2017-02-22
JP2020015747A (ja) 2020-01-30
US20250263751A1 (en) 2025-08-21
AU2020202002B2 (en) 2021-08-05
CA2933561A1 (en) 2015-06-18
KR20160103024A (ko) 2016-08-31
BR112016013516A8 (pt) 2020-05-19
CN106456793B (zh) 2021-04-30
JP2017500856A (ja) 2017-01-12

Similar Documents

Publication Publication Date Title
JP6985347B2 (ja) トランスフェクションの強化のための膜透過性ペプチドならびにそれらを使用する組成物及び方法
Gooding et al. siRNA delivery: from lipids to cell‐penetrating peptides and their mimics
JP5683533B2 (ja) 真核細胞のトランスフェクションのための新規試薬
US8759499B2 (en) Lipids for transfection of Eukaryotic cells
KR100536983B1 (ko) 유전자물질을전달용양이온비로좀
US20190352316A1 (en) Polycationic methyl phospholipids for improved delivery of nucleic acids to eukaryotic cells
AU2012268866B2 (en) Novel reagents for transfection of eukaryotic cells
Nore A synthetic cell-penetrating peptide (CPP) with protamine conjugate utilized for gene delivery

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170113

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170614

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170627

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180711

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20181002

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190110

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20190522

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190917

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20190917

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20190926

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20190930

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20191108

C211 Notice of termination of reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C211

Effective date: 20191114

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20200603

C13 Notice of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: C13

Effective date: 20210106

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20210324

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210331

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20210412

C23 Notice of termination of proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C23

Effective date: 20210422

C03 Trial/appeal decision taken

Free format text: JAPANESE INTERMEDIATE CODE: C03

Effective date: 20210527

C30A Notification sent

Free format text: JAPANESE INTERMEDIATE CODE: C3012

Effective date: 20210527

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210625

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210709

R150 Certificate of patent or registration of utility model

Ref document number: 6912887

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250