JP6906550B2 - 顕微鏡におけるライトシートジオメトリの自動調整 - Google Patents

顕微鏡におけるライトシートジオメトリの自動調整 Download PDF

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JP6906550B2
JP6906550B2 JP2018562259A JP2018562259A JP6906550B2 JP 6906550 B2 JP6906550 B2 JP 6906550B2 JP 2018562259 A JP2018562259 A JP 2018562259A JP 2018562259 A JP2018562259 A JP 2018562259A JP 6906550 B2 JP6906550 B2 JP 6906550B2
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sample
light sheet
imaging
light
image
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JP2019525216A (ja
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ヨハンネス ケラー,フィリップ
ヨハンネス ケラー,フィリップ
クマー チェトリ,ラギヴ
クマー チェトリ,ラギヴ
アラン ロイヤー,ロイック
アラン ロイヤー,ロイック
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ハワード ヒューズ メディカル インスティチュート
ハワード ヒューズ メディカル インスティチュート
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    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/32Micromanipulators structurally combined with microscopes
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/0004Microscopes specially adapted for specific applications
    • G02B21/002Scanning microscopes
    • G02B21/0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052Optical details of the image generation
    • G02B21/0076Optical details of the image generation arrangements using fluorescence or luminescence
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/06Means for illuminating specimens
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/36Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365Control or image processing arrangements for digital or video microscopes
    • G02B21/367Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/0025Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for optical correction, e.g. distorsion, aberration

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2018562259A 2016-06-24 2017-06-23 顕微鏡におけるライトシートジオメトリの自動調整 Active JP6906550B2 (ja)

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US201662354384P 2016-06-24 2016-06-24
US62/354,384 2016-06-24
US201762447154P 2017-01-17 2017-01-17
US62/447,154 2017-01-17
PCT/US2017/038970 WO2017223426A1 (en) 2016-06-24 2017-06-23 Automated adjustment of light sheet geometry in a microscope

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JP6906550B2 true JP6906550B2 (ja) 2021-07-21

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US (2) US11320640B2 (https=)
EP (1) EP3475753B1 (https=)
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Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102017106984B4 (de) * 2017-03-31 2022-02-10 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Verfahren zum Betreiben eines Lichtmikroskops mit optimierter Beleuchtungsgeometrie
WO2019075424A1 (en) * 2017-10-12 2019-04-18 Howard Hughes Medical Institute HIGH-RESOLUTION REAL-TIME IMAGING WITH ADAPTIVE OPTICAL ELEMENTS AND NETWORK LIGHT SHEETS
EP3735606B1 (en) * 2018-01-02 2023-03-22 King's College London Method and system for localisation microscopy
CN108226120B (zh) * 2018-01-23 2020-10-16 哈尔滨工业大学 一种测量片状激光光束尺寸和能量分布的装置及方法
US20210239955A1 (en) * 2018-06-08 2021-08-05 The Board Of Trustees Of The Leland Stanford Junior University Near infra-red light sheet microscopy through scattering tissues
CN108956562B (zh) * 2018-06-11 2020-05-22 浙江大学 一种基于重定位的光切片荧光显微成像方法和装置
KR102767094B1 (ko) * 2018-07-20 2025-02-13 오퍼 옵트로닉스 솔루션스 리미티드 다중-영상 레이저 빔 품질 측정의 초점 보정을 위한 방법 및 장치
EP3633414B1 (en) * 2018-10-02 2022-03-02 Vaisala, OYJ A forward scatter sensor
EP3751326B1 (en) * 2019-06-14 2025-04-09 Leica Microsystems CMS GmbH Method and microscope for imaging a sample
US11322343B2 (en) * 2019-07-09 2022-05-03 IonQ, Inc. Optical alignment using reflective dove prisms
KR102073712B1 (ko) * 2019-10-29 2020-02-05 한국기초과학지원연구원 센서리스 적응광학 시스템, 이를 적용한 이미지 장치 및 통신 장치
EP4105642A4 (en) * 2020-02-12 2023-11-29 MGI Tech Co., Ltd. Optical imaging system and biochemical substance detection system using same
CN111521608A (zh) * 2020-04-27 2020-08-11 中国科学院广州生物医药与健康研究院 一种超分辨率显微成像方法及显微镜
EP4211508B1 (en) * 2020-09-14 2025-11-26 Singular Genomics Systems, Inc. Methods and systems for multidimensional imaging
WO2022061191A1 (en) * 2020-09-20 2022-03-24 The Regents Of The University Of California Transverse sheet illumination microscopy (transim)
DE102020128524A1 (de) * 2020-10-29 2022-05-05 Carl Zeiss Microscopy Gmbh Lichtblattmikroskop und Verfahren der Lichtblattmikroskopie
WO2022157300A1 (en) * 2021-01-22 2022-07-28 Viventis Microscopy Sàrl Microscope for imaging a sample
DE102021114307A1 (de) * 2021-06-02 2022-12-08 Carl Zeiss Microscopy Gmbh Digitale kontrastnachbearbeitung von mikroskopiebildern
US20260099038A1 (en) 2022-09-26 2026-04-09 Consiglio Nazionale Delle Ricerche System and method for adjusting the alignment of a light sheet microscope
CN118414566A (zh) * 2022-11-30 2024-07-30 锘海生物科学仪器(上海)有限公司 一种平铺光片显微镜、其成像方法、显微镜系统和检测相机
EP4664180A1 (en) * 2024-06-12 2025-12-17 Viventis Microscopy Sàrl Method for acquiring images and related microscope

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001289914A1 (en) * 2000-09-25 2002-04-02 Sensovation Ag Image sensor device, apparatus and method for optical measurements
JP2003185927A (ja) 2001-12-13 2003-07-03 Olympus Optical Co Ltd 走査型レーザー顕微鏡
US20040109586A1 (en) * 2002-12-04 2004-06-10 Scott Samson Shadowed image particle profiling and evaluation recorder
DE10257423A1 (de) 2002-12-09 2004-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL) Mikroskop
US7282716B2 (en) * 2003-11-10 2007-10-16 Technology Innovations, Llc Digital imaging assembly and methods thereof
WO2009002225A2 (ru) 2007-06-25 2008-12-31 Closed Company 'molecular-Medicine Technologies' Многофункциональное устройство для диагностики и способ тестирования биологических объектов
DE102007045897A1 (de) 2007-09-26 2009-04-09 Carl Zeiss Microimaging Gmbh Verfahren zur mikroskopischen dreidimensionalen Abbildung einer Probe
US8537461B2 (en) 2007-11-26 2013-09-17 Carl Zeiss Microimaging Gmbh Method and configuration for the optical detection of an illuminated specimen
DE102007063274B8 (de) 2007-12-20 2022-12-15 Albert-Ludwigs-Universität Freiburg Mikroskop
DE102008009216A1 (de) 2008-02-13 2009-08-20 Carl Zeiss Microimaging Gmbh Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe
US9134521B2 (en) * 2008-07-30 2015-09-15 The Regents Of The University Of California Multidirectional selective plane illumination microscopy
GB0814039D0 (en) * 2008-07-31 2008-09-10 Imp Innovations Ltd Optical arrangement for oblique plane microscopy
WO2010107933A1 (en) * 2009-03-17 2010-09-23 The Uwm Research Foundation, Inc. Systems and methods for photoacoustic opthalmoscopy
US8063385B2 (en) * 2009-05-29 2011-11-22 General Electric Company Method and apparatus for ultraviolet scan planning
DE102009044984A1 (de) * 2009-09-24 2011-03-31 Carl Zeiss Microimaging Gmbh Mikroskop
DE102009044987A1 (de) 2009-09-24 2011-03-31 Carl Zeiss Microimaging Gmbh Mikroskop
DE102009044983A1 (de) 2009-09-24 2011-03-31 Carl Zeiss Microimaging Gmbh Mikroskop
WO2011059833A2 (en) 2009-10-29 2011-05-19 California Institute Of Technology Dual-mode raster point scanning/light sheet illumination microscope
US8582208B2 (en) * 2009-12-18 2013-11-12 Sagem Identification Bv Method and apparatus for manufacturing a security document comprising a lenticular array and blurred pixel tracks
US8711211B2 (en) 2010-06-14 2014-04-29 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US10051240B2 (en) 2010-06-14 2018-08-14 Howard Hughes Medical Institute Structured plane illumination microscopy
WO2012027542A2 (en) 2010-08-25 2012-03-01 California Institute Of Technology Simultaneous orthogonal light sheet microscopy and computed optical tomography
DE102010060121C5 (de) 2010-10-22 2023-09-28 Leica Microsystems Cms Gmbh SPIM-Mikroskop mit sequenziellem Lightsheet
EP2661603A4 (en) * 2011-01-06 2014-07-23 Univ California Lensless Tomographic Imaging Devices and Methods
US10908403B2 (en) 2011-02-14 2021-02-02 European Molecular Biology Laboratory (Embl) Light-pad microscope for high-resolution 3D fluorescence imaging and 2D fluctuation spectroscopy
US9316824B2 (en) 2011-03-04 2016-04-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Optomechanical module for converting a microscope to provide selective plane illumination microscopy
JP6037623B2 (ja) 2012-02-24 2016-12-07 オリンパス株式会社 レーザ走査型共焦点顕微鏡、及び、レーザ走査型共焦点顕微鏡の光学系のアライメント調整方法
ES2898616T3 (es) 2012-04-03 2022-03-08 Univ Court Univ St Andrews Imágenes de alta resolución de volúmenes extendidos
RU2014150343A (ru) * 2012-05-14 2016-07-10 Оптофлуидикс, Инк. Способы использования оптических сил ближнего поля
DE102012013163B4 (de) 2012-07-02 2022-08-25 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur Lichtscheibenmikroskopie
GB201217171D0 (en) * 2012-08-23 2012-11-07 Isis Innovation Stimulated emission depletion microscopy
US9404869B2 (en) * 2012-10-09 2016-08-02 Howard Hughes Medical Institute Multiview light-sheet microscopy
DE102012020240A1 (de) * 2012-10-12 2014-04-17 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur SPIM Mikroskopie
WO2014150536A1 (en) 2013-03-15 2014-09-25 Wayne Leonard Rodenhausen Lightsheet microscopy with rotational-shear interferometry
US9581798B2 (en) * 2013-07-22 2017-02-28 Fundacio Institut De Ciencies Fotoniques Light sheet-based imaging device with extended depth of field
US10539772B2 (en) 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
DE102013021542A1 (de) 2013-12-18 2015-06-18 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur SPIM Mikroskopie
US9500846B2 (en) 2014-03-17 2016-11-22 Howard Hughes Medical Institute Rapid adaptive optical microscopy over large multicellular volumes
CN106164736A (zh) * 2014-05-30 2016-11-23 斯坦福大学托管董事会 用于对大的完整组织样品进行成像的方法及装置
US10007100B2 (en) * 2014-11-04 2018-06-26 Olympus Corporation Light sheet illumination microscope and light sheet illumination method
US20160187633A1 (en) * 2014-12-24 2016-06-30 Applied Scientific Instrumentation Inc. Dual oblique view single plane illumination microscope

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Publication number Publication date
AU2017281533B2 (en) 2019-06-27
US20190219811A1 (en) 2019-07-18
WO2017223426A1 (en) 2017-12-28
US11320640B2 (en) 2022-05-03
AU2017281533A1 (en) 2018-12-20
US12360353B2 (en) 2025-07-15
US20220236550A1 (en) 2022-07-28
EP3475753B1 (en) 2025-04-09
EP3475753A1 (en) 2019-05-01
JP2021092575A (ja) 2021-06-17
EP3475753A4 (en) 2020-04-15
JP2019525216A (ja) 2019-09-05

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