JP6880001B2 - ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ - Google Patents

ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ Download PDF

Info

Publication number
JP6880001B2
JP6880001B2 JP2018510803A JP2018510803A JP6880001B2 JP 6880001 B2 JP6880001 B2 JP 6880001B2 JP 2018510803 A JP2018510803 A JP 2018510803A JP 2018510803 A JP2018510803 A JP 2018510803A JP 6880001 B2 JP6880001 B2 JP 6880001B2
Authority
JP
Japan
Prior art keywords
group
target
hapten
antibody
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018510803A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018528425A (ja
JP2018528425A5 (https=
Inventor
ユリ ベロスルドツェフ,
ユリ ベロスルドツェフ,
トレイシー ディー. デギア,
トレイシー ディー. デギア,
ウェンディー ジェー. フレンチ,
ウェンディー ジェー. フレンチ,
チュンシャン ハオ,
チュンシャン ハオ,
ブライアン ダニエル ケリー,
ブライアン ダニエル ケリー,
アドリアン ムリーリョ,
アドリアン ムリーリョ,
ネイサン ウォルター ポラスケー,
ネイサン ウォルター ポラスケー,
Original Assignee
ヴェンタナ メディカル システムズ, インク.
ヴェンタナ メディカル システムズ, インク.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ヴェンタナ メディカル システムズ, インク., ヴェンタナ メディカル システムズ, インク. filed Critical ヴェンタナ メディカル システムズ, インク.
Publication of JP2018528425A publication Critical patent/JP2018528425A/ja
Publication of JP2018528425A5 publication Critical patent/JP2018528425A5/ja
Application granted granted Critical
Publication of JP6880001B2 publication Critical patent/JP6880001B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02Phosphorus compounds
    • C07F9/547Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65583Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018510803A 2015-08-28 2016-08-26 ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ Active JP6880001B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562211590P 2015-08-28 2015-08-28
US62/211,590 2015-08-28
US201662301489P 2016-02-29 2016-02-29
US62/301,489 2016-02-29
PCT/US2016/049153 WO2017040349A1 (en) 2015-08-28 2016-08-26 Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens

Publications (3)

Publication Number Publication Date
JP2018528425A JP2018528425A (ja) 2018-09-27
JP2018528425A5 JP2018528425A5 (https=) 2019-10-03
JP6880001B2 true JP6880001B2 (ja) 2021-06-02

Family

ID=58188142

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2018510803A Active JP6880001B2 (ja) 2015-08-28 2016-08-26 ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
JP2019511411A Active JP7033122B2 (ja) 2015-08-28 2017-08-25 ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019511411A Active JP7033122B2 (ja) 2015-08-28 2017-08-25 ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ

Country Status (7)

Country Link
US (6) US11053266B2 (https=)
EP (1) EP3341734B1 (https=)
JP (2) JP6880001B2 (https=)
CN (3) CN107923915B (https=)
AU (3) AU2016316846B2 (https=)
CA (1) CA2996798A1 (https=)
WO (2) WO2017040349A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019532275A (ja) * 2015-08-28 2019-11-07 ヴェンタナ メディカル システムズ, インク. ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020121304A1 (en) * 2018-12-10 2020-06-18 Ariel Scientific Innovations Ltd. Therapeutic conjugates
CN110632040B (zh) * 2019-05-10 2022-03-29 长沙理工大学 一种血清中前列腺特异性抗原的分析方法
CN114303203A (zh) * 2019-08-28 2022-04-08 文塔纳医疗系统公司 使用振动光谱法评估抗原修复和靶标修复进展定量
WO2021224293A1 (en) * 2020-05-07 2021-11-11 Ventana Medical Systems, Inc. Histochemical systems and methods for evaluating egfr and egfr ligand expression in tumor samples
JP2024504944A (ja) * 2021-01-15 2024-02-02 ヴェンタナ メディカル システムズ, インク. 保存安定なケージドハプテン
CN114994329A (zh) * 2022-04-28 2022-09-02 杭州广科安德生物科技有限公司 一种检测肿瘤组织中p185蛋白与p95蛋白表达水平的方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2730523A (en) * 1952-08-16 1956-01-10 Eastman Kodak Co 5-nitrothiazoleazo-n-fluoroalkyl-aniline compounds
JP3169216B2 (ja) * 1988-07-08 2001-05-21 プロメガ・バイオサイエンシーズ・インコーポレイテッド 蛍光性ベンゾチアゾール誘導体の製法及び用途
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5182203A (en) * 1989-03-29 1993-01-26 E. I. Du Pont De Nemours And Company Bifunctional compounds useful in catalyzed reporter deposition
US5180828A (en) * 1990-02-09 1993-01-19 Molecular Devices Corporation Chromophoric reagents for incorporation of biotin or other haptens into macromolecules
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US5371241A (en) * 1991-07-19 1994-12-06 Pharmacia P-L Biochemicals Inc. Fluorescein labelled phosphoramidites
GB9517060D0 (en) * 1995-08-17 1995-10-25 Ciba Geigy Ag Acylated oligopeptide derivatives
US20030235877A1 (en) * 1996-04-30 2003-12-25 La Region Wallone Conjugates of haptens to beta-lactam derivatives and their use for detecting and/or quantifying haptens in solution and device for implementation thereof
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
ES2354598T3 (es) 1998-02-27 2011-03-16 Ventana Medical Systems, Inc. Aparato de patología molecular automatizado que tiene calefactores de portaobjetos independientes.
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US20060040319A1 (en) * 2000-01-14 2006-02-23 Tom Muir Multiple sensor-containing active modified polypeptides, preparation and uses thereof
WO2003014698A2 (en) * 2001-08-07 2003-02-20 Merck & Co., Inc. Biological assay detection method
MXPA06010782A (es) * 2004-03-23 2007-03-28 Complex Biosystems Gmbh Ligador de profarmaco.
US20060246523A1 (en) 2005-04-28 2006-11-02 Christopher Bieniarz Antibody conjugates
DK2963011T3 (en) 2005-11-23 2018-08-06 Ventana Med Syst Inc MOLECULAR CONJUGATE
WO2008063378A2 (en) 2006-11-01 2008-05-29 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
WO2008098100A2 (en) * 2007-02-07 2008-08-14 Perscitus Biosciences, Llc Detection of molecule proximity
JP2010528285A (ja) 2007-05-23 2010-08-19 ベンタナ・メデイカル・システムズ・インコーポレーテツド 免疫組織化学およびinsituハイブリダーゼーションのためのポリマー担体
CA2730565A1 (en) * 2007-07-27 2009-02-05 Ensemble Discovery Corporation Detection assays and use thereof
WO2010022332A1 (en) 2008-08-22 2010-02-25 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
CA2842661C (en) 2009-10-19 2016-02-23 Ventana Medical Systems, Inc. Imaging system and techniques
BR112012012787B1 (pt) * 2009-11-27 2019-12-17 Ecole Polytechnique Fed Lausanne Epfl biossensor para a detecção de difusão de biomoléculas fluorescentes, arranjo, conjunto e método para a detecção e medição de difusão de biomoléculas fluorescentes
ES2689593T3 (es) 2010-12-30 2018-11-14 Ventana Medical Systems, Inc. Depósito potenciado de cromógenos que utilizan análogos de piridina
US8852599B2 (en) * 2011-05-26 2014-10-07 Bristol-Myers Squibb Company Immunoconjugates, compositions for making them, and methods of making and use
JP5898318B2 (ja) 2011-09-09 2016-04-06 ベンタナ メディカル システムズ, インコーポレイテッド イメージングシステム、カセッテ、およびこれを使用する方法
EP2831587B1 (en) 2012-03-27 2018-05-16 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
DK2971064T3 (da) * 2013-03-12 2019-12-16 Ventana Med Syst Inc Nærhedsassay til in situ-påvisning af mål
CN103513039B (zh) * 2013-07-10 2015-12-23 广州美格生物科技有限公司 一种利用定向肽库检测蛋白质与其他分子相互作用的方法
EP3079772B1 (en) * 2013-12-10 2020-02-05 Merck Sharp & Dohme Corp. Immunohistochemical proximity assay for pd-1 positive cells and pd-ligand positive cells in tumor tissue
WO2015124703A1 (en) * 2014-02-24 2015-08-27 Ventana Medical Systems, Inc. Quinone methide analog signal amplification
EP3125943B1 (en) * 2014-04-04 2026-04-22 Merck Sharp & Dohme LLC Phosphate based linkers for intracellular delivery of drug conjugates
CN107003323B (zh) * 2014-11-25 2019-06-04 文塔纳医疗系统公司 使用化学连接和半抗原转移的邻近测定法
EP3037820A1 (en) 2014-12-27 2016-06-29 Miltenyi Biotec GmbH Cell detection methods and reagents having a releasable labelling moiety
CN107923915B (zh) * 2015-08-28 2021-05-04 文塔纳医疗系统公司 使用经掩蔽的半抗原在福尔马林固定石蜡包埋的组织中的蛋白质邻近测定

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019532275A (ja) * 2015-08-28 2019-11-07 ヴェンタナ メディカル システムズ, インク. ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
JP7033122B2 (ja) 2015-08-28 2022-03-09 ヴェンタナ メディカル システムズ, インク. ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
US11345719B2 (en) 2015-08-28 2022-05-31 Vetana Medical Systems, Inc. Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens
US12173019B2 (en) 2015-08-28 2024-12-24 Ventana Medical Systems, Inc. Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens
US12559511B2 (en) 2015-08-28 2026-02-24 Ventana Medical Systems, Inc. Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens
US12577266B2 (en) 2015-08-28 2026-03-17 Ventana Medical Systems, Inc. Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens

Also Published As

Publication number Publication date
US20180186821A1 (en) 2018-07-05
JP2018528425A (ja) 2018-09-27
CN116338167A (zh) 2023-06-27
US20220089622A1 (en) 2022-03-24
US12173019B2 (en) 2024-12-24
EP3341734A4 (en) 2019-02-06
AU2016316846A1 (en) 2018-02-22
WO2018039605A1 (en) 2018-03-01
CN107923915A (zh) 2018-04-17
AU2016316846B2 (en) 2021-07-08
WO2017040349A1 (en) 2017-03-09
CN107923915B (zh) 2021-05-04
US20250066397A1 (en) 2025-02-27
US11345719B2 (en) 2022-05-31
EP3341734B1 (en) 2024-08-07
US20210221833A1 (en) 2021-07-22
AU2023201682A1 (en) 2023-04-13
JP7033122B2 (ja) 2022-03-09
AU2021218128B2 (en) 2022-12-22
AU2021218128A1 (en) 2021-09-09
CA2996798A1 (en) 2017-03-09
AU2023201682B2 (en) 2023-12-07
EP3341734A1 (en) 2018-07-04
US20190233447A1 (en) 2019-08-01
US20210309680A1 (en) 2021-10-07
US12577266B2 (en) 2026-03-17
CN109791144B (zh) 2023-04-18
US11053266B2 (en) 2021-07-06
CN109791144A (zh) 2019-05-21
US12559511B2 (en) 2026-02-24
JP2019532275A (ja) 2019-11-07

Similar Documents

Publication Publication Date Title
JP6880001B2 (ja) ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
JP7021126B2 (ja) マルチ色素キノンメチド及びチラミドコンジュゲートによる発色性ihc及びish染色のための新規の色
JP2019532012A (ja) Ihc及びishアッセイにおけるシグナル増幅のためのクリックケミストリーの応用
US12173031B2 (en) Peptide nucleic acid conjugates
JP2023113659A (ja) ペプチド核酸コンジュゲート
EP3504549B1 (en) Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens
US20240002428A1 (en) Storage stable caged haptens

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190821

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190821

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20200520

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200623

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200909

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201110

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210209

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20210406

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210430

R150 Certificate of patent or registration of utility model

Ref document number: 6880001

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250