JP6858787B2 - 新たなポリリン酸依存性グルコキナーゼ及びこれを用いたグルコース6−リン酸の製造方法 - Google Patents
新たなポリリン酸依存性グルコキナーゼ及びこれを用いたグルコース6−リン酸の製造方法 Download PDFInfo
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- JP6858787B2 JP6858787B2 JP2018544874A JP2018544874A JP6858787B2 JP 6858787 B2 JP6858787 B2 JP 6858787B2 JP 2018544874 A JP2018544874 A JP 2018544874A JP 2018544874 A JP2018544874 A JP 2018544874A JP 6858787 B2 JP6858787 B2 JP 6858787B2
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- glucose
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- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- GSXOAOHZAIYLCY-HSUXUTPPSA-N keto-D-fructose 6-phosphate Chemical compound OCC(=O)[C@@H](O)[C@H](O)[C@H](O)COP(O)(O)=O GSXOAOHZAIYLCY-HSUXUTPPSA-N 0.000 description 1
- BJHIKXHVCXFQLS-PQLUHFTBSA-N keto-D-tagatose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-PQLUHFTBSA-N 0.000 description 1
- GSXOAOHZAIYLCY-PBXRRBTRSA-N keto-D-tagatose 6-phosphate Chemical compound OCC(=O)[C@@H](O)[C@@H](O)[C@H](O)COP(O)(O)=O GSXOAOHZAIYLCY-PBXRRBTRSA-N 0.000 description 1
- CTUVIUYTHWPELF-IYEMJOQQSA-L magnesium gluconate Chemical compound [Mg+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O CTUVIUYTHWPELF-IYEMJOQQSA-L 0.000 description 1
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- 238000010979 pH adjustment Methods 0.000 description 1
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- 239000012466 permeate Substances 0.000 description 1
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- 230000000865 phosphorylative effect Effects 0.000 description 1
- QVLTXCYWHPZMCA-UHFFFAOYSA-N po4-po4 Chemical compound OP(O)(O)=O.OP(O)(O)=O QVLTXCYWHPZMCA-UHFFFAOYSA-N 0.000 description 1
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- 230000003362 replicative effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
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- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000019832 sodium triphosphate Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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- 230000009466 transformation Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
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- 229910052725 zinc Inorganic materials 0.000 description 1
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Description
[反応式1]
本発明における用語「ポリヌクレオチド」とは、ヌクレオチドが共有結合によって長く鎖状に連なったヌクレオチドのポリマーであり、一般的に所定の長さ以上のDNAまたはRNA鎖を意味する。
受託番号:KCCM11814P
受託日:20160216
Claims (18)
- アナエロリネア・サーモフィラ(Anaerolinea thermophila)由来の耐熱性のポリリン酸依存性グルコキナーゼ、グルコースおよびポリリン酸を含み、
前記グルコキナーゼが、65〜70℃で最大活性を示し、60〜80℃の範囲全体で最大活性に比べて95%以上の活性を示すものである、
グルコース6−リン酸を製造するための組成物。 - 前記組成物の全容積を基準に、前記グルコース1重量%〜3重量%、前記ポリリン酸塩1〜10重量%、前記ポリリン酸依存性グルコキナーゼ10〜50U/mlを含むことでグルコース6−リン酸への転換収率が70%以上であることを特徴とする、請求項1に記載のグルコース6−リン酸を製造するための組成物。
- 前記組成物の全容積基準に、前記グルコース5重量%〜20重量%、前記ポリリン酸塩5〜12重量%、前記ポリリン酸依存性グルコキナーゼ10〜50U/mlを含むことでグルコース6−リン酸への転換収率が50%以上であることを特徴とする、請求項1に記載のグルコース6−リン酸を製造するための組成物。
- 前記組成物はマグネシウムイオンをさらに含むことを特徴とする、請求項2又は3に記載のグルコース6−リン酸を製造するための組成物。
- 前記マグネシウムイオンの濃度は0.2mM〜20mMであることを特徴とする、請求項4に記載のグルコース6−リン酸を製造するための組成物。
- 前記グルコース6−リン酸は45℃〜90℃の温度および/またはpH4〜pH10の条件で製造されたことを特徴とする、請求項1に記載のグルコース6−リン酸を製造するための組成物。
- 前記グルコースは澱粉またはセルロースを液化または糖化させたものであることを特徴とする、請求項1に記載のグルコース6−リン酸を製造するための組成物。
- アナエロリネア・サーモフィラ(Anaerolinea thermophila)由来の耐熱性のポリリン酸依存性グルコキナーゼ、グルコース及びポリリン酸を含む組成物からグルコース6−リン酸を製造する方法であって、前記グルコキナーゼが、65〜70℃で最大活性を示し、60〜80℃の範囲全体で最大活性に比べて95%以上の活性を示すものである、グルコース6−リン酸を製造する方法。
- 前記グルコースは澱粉またはセルロースを液化または糖化させたものであることを特徴とする、請求項8に記載のグルコース6−リン酸を製造する方法。
- 前記ポリリン酸塩はヘキサメタリン酸ナトリウムであることを特徴とする、請求項8に記載のグルコース6−リン酸を製造する方法。
- 前記グルコース6−リン酸の製造は45℃〜90℃の温度および/またはpH4〜pH10の条件で行われることを特徴とする、請求項8に記載のグルコース6−リン酸を製造する方法。
- 前記組成物がマグネシウムイオンをさらに含むことを特徴とする、請求項8に記載のグルコース6−リン酸を製造する方法。
- 前記ポリリン酸依存性グルコキナーゼの含量は10U/ml〜50U/mlであることを特徴とする、請求項8に記載のグルコース6−リン酸を製造する方法。
- 前記グルコースは前記組成物の重量を基準に0.1重量%〜40重量%であることを特徴とする、請求項8に記載のグルコース6−リン酸を製造する方法。
- 前記ポリリン酸塩は前記組成物の重量を基準に0.5重量%〜25重量%であることを特徴とする、請求項8に記載のグルコース6−リン酸を製造する方法。
- アナエロリネア・サーモフィラ(Anaerolinea thermophila)由来の耐熱性のポリリン酸依存性グルコキナーゼ、グルコースおよびポリリン酸を含み、
前記グルコキナーゼが、65〜70℃で最大活性を示し、60〜80℃の範囲全体で最大活性に比べて95%以上の活性を示すものである、
グルコース6−リン酸を製造するための組成物であって、
グルコース6−リン酸製造の反応温度が60〜80℃の範囲内に属することを特徴とする該組成物。 - 前記グルコースは澱粉またはセルロースを液化または糖化させたものであることを特徴とする、請求項16に記載のグルコース6−リン酸を製造するための組成物
- アナエロリネア・サーモフィラ(Anaerolinea thermophila)由来の耐熱性のポリリン酸依存性グルコキナーゼ、グルコース及びポリリン酸を含む組成物からグルコース6−リン酸を製造する方法であって、前記グルコキナーゼが、65〜70℃で最大活性を示し、60〜80℃の範囲全体で最大活性に比べて95%以上の活性を示すものであり、且つ、その反応温度が60〜80℃の範囲内に属することを特徴とするグルコース6−リン酸を製造する方法。
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