JP6832930B2 - Pd−l1陽性腫瘍組織における免疫細胞を同定する方法 - Google Patents

Pd−l1陽性腫瘍組織における免疫細胞を同定する方法 Download PDF

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JP6832930B2
JP6832930B2 JP2018526566A JP2018526566A JP6832930B2 JP 6832930 B2 JP6832930 B2 JP 6832930B2 JP 2018526566 A JP2018526566 A JP 2018526566A JP 2018526566 A JP2018526566 A JP 2018526566A JP 6832930 B2 JP6832930 B2 JP 6832930B2
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enzyme
detection
tumor
primary antibody
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チャンドラー モーガン バーチ,
チャンドラー モーガン バーチ,
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ヴェンタナ メディカル システムズ, インク.
ヴェンタナ メディカル システムズ, インク.
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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JP2018526566A 2015-11-22 2016-11-21 Pd−l1陽性腫瘍組織における免疫細胞を同定する方法 Active JP6832930B2 (ja)

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US201562258493P 2015-11-22 2015-11-22
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PCT/EP2016/078237 WO2017085307A1 (en) 2015-11-22 2016-11-21 Methods of identifying immune cells in pd-l1 positive tumor tissue

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Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150131269A (ko) 2013-03-15 2015-11-24 제넨테크, 인크. Pd-1 및 pd-l1 관련 상태를 치료하기 위한 바이오마커 및 방법
CN106604933B (zh) 2014-07-11 2021-08-10 文塔纳医疗系统公司 抗pd-l1抗体及其诊断用途
AU2017248830B2 (en) 2016-04-15 2023-03-09 Alpine Immune Sciences, Inc. CD80 variant immunomodulatory proteins and uses thereof
EP3491013A1 (en) 2016-07-28 2019-06-05 Alpine Immune Sciences, Inc. Cd155 variant immunomodulatory proteins and uses thereof
MX2019010887A (es) 2017-03-16 2019-10-15 Alpine Immune Sciences Inc Proteinas inmunomoduladoras de cb80 variante y usos de estas.
WO2019241758A1 (en) 2018-06-15 2019-12-19 Alpine Immune Sciences, Inc. Pd-1 variant immunomodulatory proteins and uses thereof
CN113544144A (zh) 2018-09-19 2021-10-22 高山免疫科学股份有限公司 变体cd80融合蛋白和相关构建体的方法和用途
EP3853610B1 (en) 2018-09-20 2024-05-22 Ventana Medical Systems, Inc. Size-based gating to analyze flow cytometry data
CN112789684B (zh) 2018-10-01 2025-05-09 文塔纳医疗系统公司 用于预测对pd-1轴导向疗法的应答的方法和系统
EP3887547A1 (en) 2018-11-29 2021-10-06 Ventana Medical Systems, Inc. Personalized ctdna disease monitoring via representative dna sequencing
CN115552248A (zh) * 2020-05-07 2022-12-30 文塔纳医疗系统公司 用于评价肿瘤样品中egfr和egfr配体表达的组织化学系统和方法
CN111830256A (zh) * 2020-07-01 2020-10-27 北京基谱生物科技有限公司 一种石蜡切片免疫荧光多重染色试剂盒及使用方法
JPWO2022059601A1 (cg-RX-API-DMAC10.html) * 2020-09-16 2022-03-24
EP4490509A1 (en) * 2022-03-07 2025-01-15 Astrazeneca AB Method for predicting patient response to immunotherapy
US20250243260A1 (en) 2022-03-07 2025-07-31 Alpine Immune Sciences, Inc. Immunomodulatory proteins of variant cd80 polypeptides, cell therapies and related methods and uses
JP2025526652A (ja) * 2022-08-08 2025-08-15 ヴェンタナ メディカル システムズ, インク. デジタル化された病理スライドの定量化のためのシステムおよび方法
JP2026519344A (ja) 2023-04-13 2026-06-16 ベンタナ メディカル システムズ, インコーポレイテッド 固定固形腫瘍に対する増殖アッセイ
WO2025193604A1 (en) 2024-03-11 2025-09-18 Ventana Medical Systems, Inc. Non-enzymatic dissociation of ffpe tissue and generation of single cells with intact cell surface markers

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69936841D1 (de) * 1998-10-26 2007-09-27 Ventana Med Syst Inc Nachweis des humanen papilloma-virus mit hilfe des papanicolau-tests
US6544798B1 (en) 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6195451B1 (en) * 1999-05-13 2001-02-27 Advanced Pathology Ststems, Inc. Transformation of digital images
US8071322B2 (en) * 2002-11-12 2011-12-06 Epitomics, Inc. Method for identifying differentially expressed proteins
US20130109019A1 (en) * 2010-07-02 2013-05-02 Adrian E. Murillo Hapten conjugates for target detection
ES2678211T3 (es) * 2012-03-27 2018-08-09 Ventana Medical Systems, Inc. Conjugados de señalización y procedimientos de uso
US9856320B2 (en) * 2012-05-15 2018-01-02 Bristol-Myers Squibb Company Cancer immunotherapy by disrupting PD-1/PD-L1 signaling
WO2014194293A1 (en) * 2013-05-30 2014-12-04 Amplimmune, Inc. Improved methods for the selection of patients for pd-1 or b7-h4 targeted therapies, and combination therapies thereof
US20160215052A1 (en) * 2013-07-24 2016-07-28 Dana-Farber Cancer Institute, Inc. Anti-galectin-1 (gal1) monoclonal antibodies and fragments thereof for neutralizing gal1
US10241115B2 (en) * 2013-12-10 2019-03-26 Merck Sharp & Dohme Corp. Immunohistochemical proximity assay for PD-1 positive cells and PD-ligand positive cells in tumor tissue
AU2015220750B2 (en) * 2014-02-24 2020-04-16 Ventana Medical Systems, Inc. Quinone methide analog signal amplification

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JP2018536163A (ja) 2018-12-06
US20180372747A1 (en) 2018-12-27
AU2016357478B2 (en) 2023-07-06
US11899016B2 (en) 2024-02-13
CA3005804C (en) 2022-03-29
WO2017085307A1 (en) 2017-05-26
EP3377530B1 (en) 2021-06-30
ES2886600T3 (es) 2021-12-20

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