JP6826647B1 - 特定の植物組織におけるフィトエンシンターゼ遺伝子発現をサイレンシング(silencing)するためのDNAカセット構築体およびその方法 - Google Patents

特定の植物組織におけるフィトエンシンターゼ遺伝子発現をサイレンシング(silencing)するためのDNAカセット構築体およびその方法 Download PDF

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JP6826647B1
JP6826647B1 JP2019184015A JP2019184015A JP6826647B1 JP 6826647 B1 JP6826647 B1 JP 6826647B1 JP 2019184015 A JP2019184015 A JP 2019184015A JP 2019184015 A JP2019184015 A JP 2019184015A JP 6826647 B1 JP6826647 B1 JP 6826647B1
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nucleotide sequence
dna cassette
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flower color
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開▲オン▼ 葉
開▲オン▼ 葉
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開▲オン▼ 葉
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
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    • C12N2310/00Structure or type of the nucleic acid
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin

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  • Plant Pathology (AREA)
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  • Physics & Mathematics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
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  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2019184015A 2019-09-06 2019-10-04 特定の植物組織におけるフィトエンシンターゼ遺伝子発現をサイレンシング(silencing)するためのDNAカセット構築体およびその方法 Active JP6826647B1 (ja)

Applications Claiming Priority (2)

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TW108132149 2019-09-06
TW108132149A TWI738052B (zh) 2019-09-06 2019-09-06 於特定植物組織中沉默八氫蕃茄紅素合成酶基因表現之dna卡匣構築體及其方法

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JP6826647B1 true JP6826647B1 (ja) 2021-02-03
JP2021040609A JP2021040609A (ja) 2021-03-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115747233A (zh) * 2022-11-25 2023-03-07 四川农业大学 一种调控草莓叶色的FaPDS基因及其应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113930434A (zh) * 2021-12-08 2022-01-14 河北农业大学 磷酸二酯酶基因及其蛋白的制备方法

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AU2006237317B8 (en) * 2005-04-19 2011-05-12 Basf Plant Science Gmbh Improved methods controlling gene expression

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115747233A (zh) * 2022-11-25 2023-03-07 四川农业大学 一种调控草莓叶色的FaPDS基因及其应用
CN115747233B (zh) * 2022-11-25 2023-10-13 四川农业大学 一种调控草莓叶色的FaPDS基因及其应用

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CN111154795A (zh) 2020-05-15
TW202111116A (zh) 2021-03-16
TWI738052B (zh) 2021-09-01

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