JP6733149B2 - Emulsified composition - Google Patents

Description

The present invention relates to an emulsified composition containing a sugar type biosurfactant compound.

The moisturizing function of the skin works by binding intercellular lipids to water, and a skin care material having a moisturizing performance close to such an original function of the skin has been required. Further, in recent years, consumers are increasingly interested in natural products due to safety concerns, and materials derived from natural products rather than synthetic products are required in the skin care field. For example, materials obtained by extracting from natural products such as lecithin and saponin are used in the skin care field. In addition, research and development for utilizing a fermented product produced by a microorganism as a skin care material have been actively conducted in recent years.

Examples of the skin care material produced by the above-mentioned microorganisms include biosurfactants, which are amphipathic substances having both a hydrophilic group and a lipophilic group and which have surface-active ability. Biosurfactants, which are surfactants derived from microorganisms, are being researched as environmentally advanced surfactants that are highly safe, have a low environmental load, and have excellent biodegradability.

Currently, biosurfactants are classified into five types: sugar type, acyl peptide type, phospholipid type, fatty acid type, and high molecular compound type. Of these, the glyco type biosurfactant is the most studied and And many types of substances produced by yeast have been reported. As such a sugar-type biosurfactant, for example, mannosylerythritol lipid (hereinafter sometimes referred to as “MEL”) has been used in the skin care field in recent years.

MEL has a 4-O-β-D-mannopyranosyl-meso-erythritol structure and a 1-O-β-D-mannopyranosyl-structure represented by the following general formula (1) as optical isomers of erythritol having a sugar skeleton. There is a meso-erythritol structure (the following general formula (2)).

It has been reported that conventional MELs having a 4-O-β-D-mannopyranosyl-meso-erythritol structure have various physiological activities including antibacterial properties, antitumor properties, and glycoprotein binding ability (non- Patent Document 1). In addition, this conventional MEL exhibits extremely peculiar self-assembly properties, and not only the slight difference in the molecular structure has a great influence on the formation of the self-assembly, but also the vesicle formation utilizing it has a dilute solution (6 3×10 ⁻² wt% or less) (Non-Patent Document 2). Further, a liquid crystal emulsification technique (Patent Document 1) using a conventional MEL bicontinuous sponge structure is also reported.

However, although MEL is a surfactant derived from microorganisms, it is said that MEL by itself does not have high ability to emulsify oil, and in the preparation of an emulsified composition, it has only been found to be used as an emulsification aid. Did not (Patent Document 2). That is, it is difficult to prepare an emulsified composition containing MEL as a main emulsifier, and no such report has been made so far.

JP, 2007-181789, A WO2011/149007

Kitamoto Dai "Oleoscience", (Japan), Japan Oil Chemists' Society, Volume 3, p663-672 (2003). Tee. T. Imura, N.; Ota, K.. K. Inoue, N.; Goat (N. Yagi), h. H. Negishi, h. H. Yanagishita, Di. D. Kitamoto, “Chemistry European Journal (Chem. Eur. J)”, (USA), Wiley, Vol. 12, p 2434-2440 (2006).

It is possible to widely use sugar-type biosurfactant compounds such as MEL, which has high biodegradability, low toxicity, environment-friendly, and new physiological functions, in the food industry, cosmetics industry, pharmaceutical industry, chemical industry, environment field, etc. Is desired.

Glycobiosurfactants such as MEL are known to have surface-active ability also because they have a lipophilic region and a hydrophilic region in the molecule, and are expected to be used as new natural product-derived emulsifiers. .. However, since MEL and the like have extremely low solubility in water and oil, it was difficult to prepare a stable emulsion composition by using MEL and the like as a main emulsifier.

Therefore, an object of the present invention is to provide a stable emulsified composition and its formulation, using a sugar-type biosurfactant such as MEL as a main emulsifier.

As a result of intensive studies, the present inventors have found that the above-mentioned problems can be solved by the means described below, and arrived at the present invention.
That is, the present invention has the following configurations.
[1] Containing (A) a sugar-type biosurfactant, (B) an oil component, (C) water, and (D) an amphoteric surfactant, and the amount of the (B) oil component being 50.0% by mass or more. The emulsified composition, wherein the mixing ratio of the (A) sugar-type biosurfactant to the (D) amphoteric surfactant is 10:1 to 1:5 (mass ratio).
[2] The emulsion composition according to [1], which is an O/D type emulsion.
[3] (A) Glycobiosurfactant consists of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), and mannosyl ribitol lipid (MRL). The emulsified composition according to [1] or [2], which is one or more compounds selected from the group.
[4] The glycosylated biosurfactant (A) is characterized by being mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B) and/or mannosyl erythritol lipid C (MEL-C). The emulsified composition according to [3].
[5] The amphoteric surfactant is a sulfate ester-type amphoteric surfactant, a sulfonate-type amphoteric surfactant, a phosphate ester salt-type amphoteric surfactant, an imidazoline-type amphoteric surfactant, a betaine-type amphoteric surfactant. And the emulsified composition according to any one of [1] to [4], which is one or more surfactants selected from the group consisting of an amidoamine oxide type amphoteric surfactant.
[6] The emulsion composition according to any one of [1] to [5], which further contains (E) a polyhydric alcohol.
[7] An O/W emulsion composition comprising the emulsion composition according to any one of [1] to [6] diluted with water.
[8] (A) Sugar-type biosurfactant, (C) water, and (D) amphoteric surfactant are mixed and dissolved, and then (B) an oil component is added to 50.0 mass% or more while stirring. A method for producing an emulsified composition, which is characterized.
[9] (A) Sugar-type biosurfactant, (C) water and (D) amphoteric surfactant are mixed and dissolved, and further (E) polyhydric alcohol is dissolved, and then (B) oily component is added to 50 while stirring. The method for producing an emulsified composition according to [8], which is added so as to be 0.0% by mass or more.
[10] The method for producing the emulsified composition according to [8] or [9], further including a step of diluting with water.

According to the present invention, a stable emulsified composition can be prepared using a sugar-type biosurfactant such as MEL as a main emulsifier. Further, this emulsified composition is not only stable, but also capable of emulsifying various types of oily components.

The emulsion composition and the formulation thereof provided by the present invention have effects such as a moisturizing action, a cell activating action, an anti-aging action, a hair-growing action and a rough skin improving action. Further, it is expected that the effect is improved by adding various compounding ingredients. This emulsified composition can be provided as cosmetics, quasi drugs, medical supplies, hygiene products, and pharmaceuticals.

Further, since the emulsion composition of the present invention has detergency, moisturizing action, cell activating action, anti-aging action, hair growth action, skin roughening improving action and the like, it is expected to be used as a blending component of cosmetics for washing. ..

Since the biosurfactant is of biological origin, it is excellent in safety, and the composition of the present invention has an effect of being sufficiently durable for long-term use. Further, since biosurfactants can be produced by culturing microorganisms, raw material costs are low and mass production is possible. Therefore, the composition according to the present invention has an effect that it can be provided at a low price.

(1) Emulsified composition One of the embodiments of the present invention is an emulsified composition containing a sugar-type biosurfactant, an oily component, water, and an amphoteric surfactant as essential components.

The form of the "emulsified composition" of the present invention is not particularly limited as long as it is a dispersion system containing a sugar type biosurfactant, an oil component, water, and an amphoteric surfactant as essential components, but preferably a surfactant phase ( It is an O/D type emulsion in which the D phase) is the continuous phase and the oil phase is the dispersed phase. The emulsified composition of the present invention preferably exhibits a translucent to transparent gel form or a cloudy gel form.

(1-1) Glycotype biosurfactant "Biosurfactant" is a generic term for substances produced by living organisms that have surface-active ability and emulsifying ability, and not only exhibit excellent surface-active ability and high biodegradability. Since it has various physiological actions, it may exhibit behavior and functions different from those of synthetic surfactants.

The component (A) sugar type biosurfactant used in the present invention is not particularly limited, and examples thereof include MAL (mannosyl alditol lipid). Examples of MAL include mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), and mannosyl ribitol lipid (MRL). Among them, MEL is particularly preferable. preferable.

(1-1-1) MEL
The structure of MEL is shown in general formula (3). In the general formula (3), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms, which may be the same or different. MEL is classified into four types of MEL-A, MEL-B, MEL-C, and MEL-D based on the presence or absence of acetyl groups at the 4- and 6-positions of mannose.

Specifically, in MEL-A, in the general formula (3), the substituents R2 and R3 are both acetyl groups. In MEL-B, in the general formula (3), the substituent R2 is an acetyl group and the substituent R3 is hydrogen. In MEL-C, in the general formula (3), the substituent R2 is hydrogen and the substituent R3 is an acetyl group. In MEL-D, in the general formula (3), the substituents R2 and R3 are both hydrogen.

The carbon number of the substituent R1 in the above MEL-A to MEL-D is the carbon number of the fatty acid constituting the triglyceride which is an oil and fat to be contained in the MEL production medium, and the degree of assimilation of the fatty acid of the MEL producing bacterium to be used. It depends on Further, in the case where the triglyceride has an unsaturated fatty acid residue, if the MEL-producing bacterium does not assimilate the double bond portion of the unsaturated fatty acid, it is possible to include an unsaturated fatty acid residue as the substituent R1. is there. As is clear from the above description, the obtained MEL is usually in the form of a mixture of compounds having different fatty acid residue moieties of the substituent R1.

The emulsion composition of the present invention contains MEL having a structure represented by the general formula (4) or the general formula (5). In the general formula (4), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different, and the substituent R2 is hydrogen or an acetyl group which may be the same or different. The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms. Further, in the general formula (5), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different, and the substituent R2 is hydrogen or an acetyl group which may be the same or different. The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms.

The substituents R1 in the general formulas (4) and (5) are the same or different aliphatic acyl groups having 4 to 24 carbon atoms. The carbon number of the substituent R1 is not particularly limited as long as it is within the above range, but it is more preferably 8 to 14 carbon atoms.

In addition, the substituent R1 in the general formulas (4) and (5) may be a saturated aliphatic acyl group or an unsaturated aliphatic acyl group, and is not particularly limited. When it has an unsaturated bond, it may have a plurality of double bonds, for example. The carbon chain may be linear or branched. Further, in the case of an oxygen atom-containing hydrocarbon group, the number and position of oxygen atoms contained are not particularly limited.

The fatty acid introduced into the erythritol part of MEL may be a long-chain hydrocarbon monovalent carboxylic acid. Further, it may be a saturated fatty acid or an unsaturated fatty acid. In the case of unsaturated fatty acid, it may have a plurality of double bonds. The carbon chain may be linear or branched. Furthermore, a fatty acid derivative which is a derivative of a fatty acid may be used in the present invention, or a mixture of a fatty acid and a fatty acid derivative may be used in the present invention. The fatty acid or fatty acid derivative introduced into the erythritol part of MEL is preferably derived from oils, higher fatty acids and synthetic esters.

(1-1-2) MAL other than MEL
The structure of MAL (MML, MAraL, MRL, MSL) other than MEL is shown in the general formula (6) (in the formula, the substituents R2 are hydrogen or acetyl groups which may be the same or different). In these MALs, mannitol, arabitol, ribitol, and sorbitol are added as sugar alcohols (alditols) instead of erythritol (n=4: mannitol, sorbitol, n=2: arabitol, ribitol). Corresponding to the general formula (6), MAL is a saturated or unsaturated straight chain having 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, more preferably 6 to 14 carbon atoms in the 2-position and 3-position of mannose. It has an alkanoyl group having a chain or a branch (in the formula, the substituents R2 are the same or different hydrogen or acetyl groups).

(In the formula, the substituents R1 may be the same or different and have a saturated or unsaturated straight chain or branched chain having 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, and more preferably 6 to 14 carbon atoms. (A compound having an alkanoyl group, in which the substituents R2 may be the same or different, hydrogen or an acetyl group. Preferably, in the formula, both of the substituents R2 are acetyl groups.)

The biosurfactant preferably used in the present invention is MEL-A, MEL-B or MEL-C, more preferably MEL-B having a structure represented by the general formula (7) or (8). .. More preferred is MEL-B having a structure represented by the general formula (8).

(In the general formulas (7) and (8), the substituents R1 are the same or different aliphatic acyl groups having 4 to 24 carbon atoms)

The biosurfactant may be used alone, or two or more kinds of biosurfactants may be used in combination.

The present invention provides an emulsified composition containing MEL as a main emulsifying agent. However, the amount of MEL added may be such that the action of MEL as an emulsified composition is not impaired, and the emulsion composition is stable. From the viewpoint of properties, the lower limit is usually 0.1% by mass, more preferably 0.2% by mass, further preferably 0.5% by mass, and further preferably 1.0% by mass. On the other hand, the preferable upper limit is 20% by mass, more preferably 10% by mass, further preferably 5.0% by mass, and further preferably 3.0% by mass. Here, the form of use of the MEL added to the composition of the present invention is arbitrary. For example, MEL may be used as it is as an extract from a culture solution, or a purified high-purity product, or it may be suspended in water or dissolved in a solvent before use.

(1-3) Oily Component The oily component (B) of the present invention is not particularly limited, and examples thereof include an oily component which can be used in cosmetics and which easily blends into the skin. The oily component used in the present invention may be not only a compound but also a natural oily component. As the oily component suitable for the present invention, for example, macadamia nut oil, avocado oil, olive oil, rapeseed oil, castor oil, safflower oil, cottonseed oil, jojoba oil, coconut oil, palm oil, liquid lanolin, hydrogenated coconut oil, hydrogenated oil, Oils and waxes such as hydrogenated castor oil, beeswax, candelilla wax, carnauba wax, lanolin, reduced lanolin, hard lanolin, jojoba wax, liquid paraffin, squalane, paraffin, ceresin, petrolatum, hydrocarbons such as microcrystalline wax, oleic acid Higher fatty acids such as isostearic acid, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, and undecylenic acid, cetyl alcohol, stearyl alcohol, isostearyl alcohol, behenyl alcohol, octyldodecanol, myristyl alcohol, cetostearyl alcohol, etc. Higher alcohols, cetyl isooctanoate, isopropyl myristate, hexyldecyl isostearate, diisopropyl adipate, di-2-ethylhexyl sebacate, cetyl lactate, diisostearyl malate, ethylene glycol di-2-ethylhexanoate, dicaprin Acid neopentyl glycol, di-2-heptylundecanoate glycerin, tri-2-ethylhexanoate glycerin, tri-2-ethylhexanoate trimethylolpropane, triisostearate trimethylolpropane, tetra-2-ethylhexanoate pentane Synthetic ester oils such as trit, chain polysiloxanes such as dimethylpolysiloxane, methylphenylpolysiloxane, diphenylpolysiloxane, cyclic polysiloxanes such as octamethylcyclotetrasiloxane, decamethylcyclopentasiloxane, dodecamethylcyclohexanesiloxane, amino One or more oily components selected from the group consisting of oils such as silicone oils such as modified polysiloxanes, polyether modified polysiloxanes, alkyl modified polysiloxanes, modified polysiloxanes such as fluorine modified polysiloxanes can be employed. ..

The content of the oil component of the component (B) of the present invention is not particularly limited, but from the viewpoint of stability of the emulsion composition, a generally preferred lower limit is 50.0% by mass, more preferably 60. It is 0% by mass, and more preferably 70.0% by mass. On the other hand, the preferable upper limit is 90.0% by mass, and more preferably 80.0% by mass.

(1-4) Water The component (C) water used in the present invention is not particularly limited as long as it is clean enough to be suitable for the intended use of the emulsion composition. Examples include plant-derived steam-distilled water such as deionized water, distilled water, purified water, deep sea water, hot spring water, rose water, and lavender water. Among these, one kind or a combination of two or more kinds is used. Can be used.

The content of the component (C) of this component is not particularly limited and may be appropriately determined depending on other components. That is, the remaining amount is obtained by removing the total of the mass% of (A), (B), (D) and other additives described separately. From the viewpoint of the stability of the emulsified composition, the generally preferred lower limit is 0.01% by mass, more preferably 0.1% by mass, and further preferably 1.0% by mass. On the other hand, the preferable upper limit is 20% by mass, more preferably 10% by mass, and further preferably 5% by mass.

(1-5) Amphoteric Surfactant The (D) amphoteric surfactant used in the present invention is not particularly limited, but it is a sulfate ester type amphoteric surfactant, a sulfonate type amphoteric surfactant, Examples thereof include phosphate ester salt type amphoteric surfactants such as lecithin, imidazoline type surfactants, betaine type surfactants, and amidoamine oxide type surfactants. These amphoteric surfactants can be used alone or in combination of two or more. These may be selected from components commonly used in cosmetics. Further, the use form of the amphoteric surfactant added to the composition of the present invention is arbitrary, for example, solid/flake form, solid/flake form in a solvent such as water at room temperature to high temperature. Alternatively, an aqueous solution dissolved by the above method or a commercially available product previously dissolved in a solvent such as water may be used.

The amphoteric surfactant of the component (D) of the present invention has a very high solubility for the component (A), and has an excellent effect as a component for dissolving and dispersing the component (A) in water. In order to obtain an emulsified composition containing a high concentration of oily component according to the present invention, the component (A) needs to be efficiently dissolved in a solvent. The formulation of the component (D) is very important because it helps the efficient dissolution of the component (A). Usually, the preferred lower limit is 0.01% by mass, more preferably 0.1% by mass, and further preferably 0.5% by mass. On the other hand, the preferable upper limit is 10.0% by mass, more preferably 5.0% by mass, and further preferably 2.0% by mass.

The emulsion composition in the present invention is not particularly limited in the compounding ratio of the component (A) and the component (D), but it can be obtained preferably at 10:1 to 1:5 (mass ratio) and stability And, from the viewpoint of usability, it is more preferably 5:1 to 1:2 (mass ratio).

As the content of the component (A) decreases, the effect of improving stability and usability is expected by increasing the blending ratio of the component (D) to the component (A). Alternatively, as the content of the component (A) decreases, the effect of improving the stability and usability is expected by increasing the content of the component (E).

(1-6) Polyhydric Alcohol The emulsion composition of the present invention may further contain the component (E) polyhydric alcohol. The polyhydric alcohol used in the present invention is a dihydric or higher polyhydric alcohol having two or more hydroxyl groups in the same molecule, and examples thereof include a dihydric alcohol (glycol) and a sugar alcohol. For example, one or more selected from the group consisting of glycerin, diglycerin, propanediol, propylene glycol, dipropylene glycol, butylene glycol, pentylene glycol, hexylene glycol, polyethylene glycol, sorbitol, maltitol, sucrose, erythritol and xylitol. Can be used.

The content of the component (E) polyhydric alcohol of the present invention is not particularly limited, but from the viewpoint of the stability of the emulsified composition, the preferred lower limit is usually 5.0% by mass, and more preferably 10%. It is 0.0% by mass. On the other hand, the preferable upper limit is 30.0% by mass, and more preferably 20.0% by mass.

(1-7) Other components In addition to the components described above, the emulsion composition of the present invention may contain various components used in ordinary cosmetics, quasi drugs, pharmaceuticals and the like. Of course, for example, a moisturizer, a thickener, a medicinal component, an antiseptic, a pigment, a powder, a pH adjuster, an ultraviolet absorber, an antioxidant, a fragrance and the like can be appropriately added.

As the above-mentioned moisturizer, any component may be used as long as it is a component generally used in cosmetics, and examples thereof include pyrrolidonecarboxylic acid, lactic acid, sodium lactate, amino acids, sodium hyaluronate, and sodium chondroitin sulfate. To be Addition of these moisturizers is expected to have effects such as improving the feeling of use and imparting moisturizing properties to the emulsion composition of the present invention.

As the above-mentioned thickener, any of the components commonly used in cosmetics may be used, for example, guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, Curdlan, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, methyl hydroxypropyl cellulose, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, locust bean gum, succinoglucan, caronic acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, Examples thereof include polyvinylpyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, sodium polyacrylate and bentonite. These thickeners are expected to improve the stability of the emulsion composition of the present invention.

As the above-mentioned medicinal component, any component commonly used in cosmetics may be used. For example, vitamin A or its derivative, vitamin B6 or its derivative, vitamin B2 or its derivative, vitamin B12, Vitamin Bs such as vitamin B15 or its derivatives, α-tocopherol, β-tocopherol, γ-tocopherol, vitamin E such as vitamin E acetate, vitamin Ds, vitamin H, pantothenic acid, pantethine, pyrroloquinoline quinone and other vitamins. And various animal and plant extracts. Addition of these medicinal components is expected to impart an antioxidant effect or an anti-aging effect to the emulsion composition of the present invention.

As the above-mentioned preservative, any of components commonly used in cosmetics may be used, and examples thereof include parabens such as methylparaben, ethylparaben, propylparaben, and butylparaben, phenoxyethanol, and ethylhexylglycerin. Can be mentioned. Addition of these antiseptics is expected to have the effect of improving antiseptic properties of the emulsion composition of the present invention.

As the above-mentioned pH adjusting agent, any component generally used in cosmetics may be used, and examples thereof include citric acid, Na citrate, lactic acid, Na lactate, Na hydroxide, K hydroxide and the like. Are listed. Addition of these pH adjusting agents is expected to have effects such as stability improvement on the emulsion composition of the present invention.

By adding various components as described above to the emulsified composition of the present invention, depending on those components, moisturizing action, cell activating action, anti-aging action, hair-growth action and rough skin improving action are imparted. Can be expected to do.

The method for preparing the emulsified composition of the present invention is a method in which a sugar-type biosurfactant compound, water and an amphoteric surfactant are mixed and dissolved, and an oily component is gradually added while vigorously stirring to obtain an emulsified composition. It is preferable for stabilizing the product. When a polyhydric alcohol is used, the sugar-type biosurfactant compound, water and an amphoteric surfactant are mixed and dissolved, then the polyhydric alcohol is added and mixed and dissolved again, and then the oily component is gradually added while vigorously stirring. A method of adding to obtain an emulsified composition is preferable. These methods can be combined with mechanical emulsification in which a propeller mixer, a paddle mixer, a disper mixer, a homogenizer or the like is used for stirring, mixing and emulsifying.

The appearance of the emulsion composition obtained in the present invention may be transparent, slightly cloudy or cloudy, and the physical properties may be any of liquid, gel, cream and solid.

The emulsion composition obtained by the present invention may be any type of W/O type, O/W type or O/D type emulsion composition, but the emulsion composition produced by the above method is preferably O/O type. A D-type emulsion is shown.

The emulsion composition obtained as described above can be diluted with water to obtain an emulsion composition different from the above. An emulsion composition that is an O/W emulsion can be obtained by diluting an emulsion composition that is an O/D emulsion with water. The diluting solvent is not particularly limited to water, and water-soluble components generally used in cosmetics may be mixed. That is, as the diluting solvent, there is no problem even if one or a mixture of two or more water-soluble components is used.

The appearance of the emulsion composition obtained by dilution may be slightly cloudy or cloudy, and the physical properties may be any of liquid, gel, cream, and solid.

From the viewpoint of stability and usability of the emulsified composition after dilution, the appearance of the emulsified composition before dilution is preferably a transparent gel, and by diluting the emulsified composition in this state, a fine emulsion is obtained. It is expected to obtain a milky white emulsion composition consisting of

The emulsion composition obtained by the present invention can be applied to general cosmetics. Specifically, hair cosmetics such as shampoos, conditioners, treatments, facial cleansers, cleansing cosmetics, sunscreen cosmetics, packs, massage cosmetics, skin cosmetics such as lotion, emulsion, cream, eyeliner, mascara. , Makeup cosmetics such as lipsticks and foundations, and bath cosmetics.

Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is not limited to the examples.

(Production example)
Production Example 1 Production of MEL-A Pseudozyma antarctica (NBRC 10736) was cultivated in a YM medium at a culturing temperature of 26° C. for 48 hours with aeration and stirring using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The resulting seed culture was subjected to aeration and agitation culture in a YM medium (containing 5% olive oil) at a culture temperature of 26° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture solution, and the mixture was stirred and distributed. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer, and the mixture was left standing for 30 minutes and then concentrated by heating to obtain crude MEL-A. Using a silica gel column, the obtained crude MEL-A was subjected to chloroform:acetone=1:0, chloroform:acetone=9:1, chloroform:acetone 1:1, chloroform:acetone=3:7, chloroform:acetone=. Elute at 0:1. The MEL-A fraction was collected and concentrated to obtain purified MEL-A.

Production Example 2 Production of MEL-B Pseudozyma tsukubaensis (NBRC 1940) was subjected to aeration stirring culture in a YM medium at a culture temperature of 26° C. for 48 hours using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The resulting seed culture was subjected to aeration and agitation culture in a YM medium (containing 5% olive oil) at a culture temperature of 26° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture solution, and the mixture was stirred and distributed. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to stand for 30 minutes and then concentrated by heating to obtain crude MEL-B. Using a silica gel column, the obtained crude MEL-B was subjected to chloroform:acetone=1:0, chloroform:acetone=9:1, chloroform:acetone=1:1, chloroform:acetone=3:7, chloroform:acetone=. Elute at 0:1. The MEL-B fraction was collected and concentrated to obtain purified MEL-B.

Production Example 3 Production of MEL-C Pseudozyma hubeiensis strain KM-59 (FERM-20987) was subjected to aeration stirring culture in a YM medium at a culture temperature of 26° C. for 48 hours using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The resulting seed culture was subjected to aeration stirring culture in a YM medium (containing 5% olive oil) at a culture temperature of 26° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture solution, and the mixture was stirred and distributed. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer and the mixture was allowed to stand for 30 minutes and then concentrated by heating to obtain crude MEL-C. Using a silica gel column, the obtained crude MEL-C was subjected to chloroform:acetone=1:0, chloroform:acetone=9:1, chloroform:acetone 1:1, chloroform:acetone=3:7, chloroform:acetone=. Elute at 0:1. The MEL-C fraction was collected and concentrated to obtain purified MEL-C.

(Example)
Using the sugar-type biosurfactant compounds obtained in the above Production Examples 1 to 3, emulsion compositions of Examples 1 to 16 and Comparative Examples 1 to 5 were prepared with the components and blending ratios shown in Tables 1 to 4.

(Conditions when preparing an emulsified composition)
MEL, purified water, and various amphoteric surfactants were heated and dissolved at 50 to 60° C. and mixed at a mixing ratio shown in Tables 1 to 4 below, and a polyhydric alcohol (glycerin) was added as necessary. , And then uniformly mix again, and then gradually add various oil components (squalane, dimethylpolysiloxane, olive oil or tri(capryl/capric acid)glyceryl) while stirring with a propeller mixer (1,000 rpm) to obtain an emulsion composition. Was prepared. MEL-A, MEL-B, and MEL-C were used as MEL. As various amphoteric surfactants, cocamidopropyl betaine, lauramidopropyl hydroxysultaine, sodium cocoamphoacetate, and lauramidopropylamine oxide were used.

(Evaluation method)
Regarding the obtained emulsified composition, the properties of the emulsified composition immediately after production and the stability when the emulsified composition was stored in a thermostat at 25° C. for 6 months were also shown in Tables 1 to 4. The properties were evaluated by visually observing the emulsified or separated state.
⊚: High transparency and maintains gel state ○: Semi-transparent to slightly turbid, but maintains gel state ×: Separates and does not form gel state

Further, the emulsion composition obtained by diluting the emulsion composition 10 times was also evaluated by its properties. When the emulsified composition was added to an aqueous solution containing 0.2% by mass of carboxyvinyl polymer (final concentration) so as to be 10% by mass, mixed by a standard method, and then stored in a thermostat at 25° C. for 6 months. The stability is also shown in Tables 1 to 4 as "diluent properties (25°C, 6 months)". The properties were evaluated by visually observing the emulsified or separated state.
⊚: Maintains a good milky white emulsified condition ○: Maintains a milky white to white emulsified condition ×: Separation

In Comparative Examples 1 to 5, when MEL-B, an amphoteric surfactant (cocamidopropyl betaine), an oil component (squalane) or water was not added, or the oil component (squalane) was 40%. When the content was relatively low (% by mass), the mixture did not form a gel and separated immediately after preparation.

On the other hand, in Examples 1 to 14, by adding MEL-B, the amphoteric surfactant, the oil component and water as essential components, stable gel-like emulsion compositions could be obtained. In Example 15, a stable gel emulsion composition could be obtained by adding MEL-A, an amphoteric surfactant, an oil component and water as essential components. Furthermore, in Example 16, a stable gel emulsion composition could be obtained by adding MEL-C, an amphoteric surfactant, an oil component and water as essential components.

In the examples, as the amphoteric surfactant, lauramidopropyl hydroxysultaine classified as a sulfobetaine type, cocoamphoacetate Na classified as an imidazoline type, lauramidopropylamine oxide classified as an amidoamine oxide type, and amidopropyl are used. Although the emulsified composition of the present invention was obtained using cocamidopropyl betaine classified as a betaine type, it is possible to obtain the emulsified composition of the present invention also with respect to these components having similar structure to those of the amphoteric surfactant. It is believed that there is.

In the examples, squalane, dimethylpolysiloxane, olive oil, and glyceryl tri(capryl/caprate) were used as the oil component to obtain the emulsion composition of the present invention, which contained components similar to these oil components. It is considered possible to obtain the emulsified composition of the present invention with respect to oily components as well.

According to the present invention, an emulsified composition containing MEL, which is a sugar-type biosurfactant compound, as a main emulsifier can be prepared. The emulsified composition of the present invention can emulsify various types of oily ingredients. With this emulsion composition and its formulation, a very useful cosmetic preparation can be prepared, and it is expected to make a great contribution to the industry.

Claims (6)

(A) one or two or more compounds selected from the group consisting of mannosyl erythritol lipid B (MEL-B) and mannosyl erythritol lipid C (MEL-C), a glycosylated biosurfactant, (B) an oil component, (C) Water, (D) Amphoteric surfactant, and (E) Glycerin are contained, the compounding amount of (B) oil component is 50.0 mass% or more, and (A) sugar type biosurfactant and ( Emulsion composition which is an O/D type emulsion whose compounding ratio of D) amphoteric surfactant is 10:1 to 1:2.5 (mass ratio). The emulsion composition according to claim 1, which is an O/D emulsion in which the (A) sugar-type biosurfactant is mannosyl erythritol lipid B (MELB). Amphoteric surfactants are sulfate ester type amphoteric surfactants, sulfonate type amphoteric surfactants,
One or more surfactants selected from the group consisting of phosphate ester salt type amphoteric surfactants, imidazoline type amphoteric surfactants, betaine type amphoteric surfactants and amidoamine oxide type amphoteric surfactants, The emulsified composition according to claim 1 or 2, which is an O/D type emulsion. The amphoteric surfactant is one or more surfactants selected from the group consisting of cocamidopropyl betaine, lauramidopropyl hydroxysultaine, sodium cocoamphoacetate, and lauramidopropylamine oxide, O/D The emulsified composition according to claim 3, which is a type emulsion. (A) Glycobiosurfactant, which is one or more compounds selected from the group consisting of mannosyl erythritol lipid B (MEL-B) and mannosyl erythritol lipid C (MEL-C), (C) water and ( D) An amphoteric surfactant is mixed and dissolved, and (E) glycerin is further dissolved, and then (B) an oil component is added with stirring so as to be 50.0% by mass or more, which is an O/D type emulsion. A method for producing a composition. A method for producing an emulsified composition, which is an O/W emulsion, further comprising a step of diluting the O/D emulsion according to claim 5 with water.