JP6531514B2 - Emulsified composition - Google Patents

Description

  The present invention relates to an emulsion composition containing a glycobiosurfactant compound.

  The moisturizing function of the skin functions by intercellular lipid binding to water, and there has been a need for a skin care material having moisturizing ability close to the function of such original skin. Also, in recent years, consumers have become more interested in natural products due to safety concerns, and materials derived from natural products rather than synthetic products are required in the skin care field. For example, materials obtained by extraction from natural products, such as lecithin and saponin, are used in the skin care field. In addition, research and development for utilizing fermented substances produced by microorganisms as skin care materials has been actively conducted in recent years.

  Examples of skin care materials produced by the aforementioned microorganisms include amphiphilic substances having both hydrophilic and lipophilic groups, and biosurfactants having surface activity. Biosurfactants, which are surfactants derived from microorganisms, are being studied as environmentally advanced surfactants that are highly safe and have a low impact on the environment and are excellent in biodegradability.

At present, biosurfactants are classified into five types: glycoform, acyl peptide type, phospholipid type, fatty acid type and polymer compound type, among which the glycobiosurfactant, among these, is the best studied and bacteria. And many types of substances produced by yeast have been reported. As such glycotype biosurfactant, for example, mannosyl erythritol lipid (hereinafter sometimes referred to as "MEL") has recently been used in the skin care field.

  In MEL, 4-O-β-D-mannopyranosyl-meso-erythritol structure and 1-O-β-D-mannopyranosyl- as shown in the following general formula (1) as an optical isomer of erythritol with a sugar skeleton The meso-erythritol structure (following general formula (2)) exists.

 The MEL having a conventional 4-O-β-D-mannopyranosyl-meso-erythritol structure has been reported to have various physiological activities including antibacterial activity, antitumor activity, and glycoprotein binding ability (non- Patent Document 1). Moreover, this conventional MEL exhibits extremely unique self-assembly properties, and slight differences in molecular structure not only greatly affect the formation of self-assembly, but also dilute solution (6) for vesicle formation utilizing it. (3.times.10@-2 wt% or less) is reported only (Non-Patent Document 2). Furthermore, a liquid crystal emulsification technique (Patent Document 1) using a conventional MEL bicontinuous sponge structure is also reported.

 However, although MEL is a surfactant derived from a microorganism, it is considered that the ability to emulsify the oil is not high by itself, and in the preparation of an emulsion composition, it finds use only as an emulsification aid. (Patent Document 2). That is, it is difficult to prepare an emulsion composition containing MEL as the main emulsifier, and such a report has not been made at all.

JP 2007-181789 A WO2011 / 149007

Dai Kitamoto "Oleo Science", (Japan), The Japan Oil Chemistry Association, vol. 3, p663-672 (2003). Te. Imura (T. Imura), N. Ohta (K.). Inoue, N. Goat (N. Yagi), H. Negishi (H. Negishi), H. Yanagishita (H. Yanagishita), di. Kitamoto (D. Kitamoto) "Chemistry a European Journal (Chem. Eur. J), (United States), Wiley, Vol. 12, p 2434-2440 (2006).

 To widely use sugar-type biosurfactant compounds such as MEL that have high biodegradability, low toxicity, environmental friendliness, and novel physiological functions in food industry, cosmetics industry, pharmaceutical industry, chemical industry, environmental field, etc. It is desired.

 Glycol-type biosurfactants such as MEL are known to have surface activity because they have both a lipophilic region and a hydrophilic region in the molecule, and are expected to be used as novel natural product-derived emulsifiers. . However, since MEL and the like have extremely low solubility in water and oil, it has been difficult to prepare a stable emulsion composition using MEL and the like as a main emulsifier.

 Therefore, an object of the present invention is to provide a stable emulsion composition and a formulation thereof using a sugar type biosurfactant such as MEL as a main emulsifier.

MEANS TO SOLVE THE PROBLEM As a result of earnestly examining, the present inventors found out that the said subject could be solved by the means shown below, and arrived at this invention.
That is, the present invention is configured as follows.
[1] Contains (A) glycoform biosurfactant, (B) oil component, (C) water, and (D) glycyrrhizinate, and the blending amount of (B) oil component is 50.0 mass% or more An emulsifying composition characterized in that the compounding ratio of (A) glycobiosurfactant to (D) glycyrrhizinate is 10: 1 to 1: 5 (mass ratio).
[2] The emulsion composition according to [1], which is an O / D type emulsion.
[3] (A) Glycotype biosurfactant consists of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL) It is 1 type, or 2 or more types of compounds selected from the group, The emulsion composition as described in [1] or [2] characterized by the above-mentioned.
[4] The emulsion composition according to [3], wherein the sugar type biosurfactant is mannosyl erythritol lipid B (MEL-B) and / or mannosyl erythritol lipid C (MEL-C).
[5] The emulsion composition according to any one of [1] to [4], which further contains (E) a polyhydric alcohol.
[6] An O / W emulsion composition comprising the emulsion composition of any one of [1] to [5] diluted with water.
[7] It is characterized in that (A) glycoform biosurfactant, (C) water and (D) glycyrrhizinate are mixed and dissolved, and then (B) an oily component is added so as to be 50.0 mass% or more while stirring. Method for producing an emulsified composition as claimed in
[8] (A) A sugar-type biosurfactant, (C) water and (D) glycyrrhizinate are mixed and dissolved, and (E) a polyhydric alcohol is dissolved, and then (B) an oily component is mixed with stirring 50. It adds so that it may become 0 mass% or more, The manufacturing method of the emulsion composition as described in [7] characterized by the above-mentioned.
[9] The method for producing an emulsion composition according to [7] or [8], further comprising the step of diluting with water.

  According to the present invention, a stable emulsion composition can be prepared using a sugar-type biosurfactant such as MEL as a main emulsifier. In addition to being stable, this emulsion composition is capable of emulsifying various types of oil components.

  The emulsified composition provided by the present invention and the formulation thereof have effects such as moisturizing action, cell activating action, anti-aging action, hair growth action and rough skin improving action. Moreover, the improvement of the effect is anticipated by adding various compounding components. The emulsified composition can be provided as cosmetics, quasi-drugs, medical supplies, hygiene products, and pharmaceuticals.

In addition, since the emulsified composition of the present invention has detergency, moisturizing action, cell activating action, anti-aging action, hair growth action and rough skin improving action, etc., it is also expected to be used as a component of cleansing cosmetics .

  Since the biosurfactant is derived from an organism, it is excellent in safety, and the composition according to the present invention has the effect of being sufficiently resistant to long-term use. Furthermore, since biosurfactants can be produced by culturing microorganisms, the raw material cost is low and mass production is possible. Therefore, the composition according to the present invention can be provided at low cost.

(1) Emulsified Composition One of the embodiments of the present invention is an emulsified composition containing a sugar type biosurfactant, an oily component, water, and glycyrrhizinate as essential components.

  The form of the "emulsified composition" of the present invention is not particularly limited as long as it is a dispersion system containing sugar type biosurfactant, oily component, water, and glycyrrhizinate as essential components, but preferably surfactant phase (D Phase) is a continuous phase, and an oil phase is a dispersed phase. The emulsion composition of the present invention preferably exhibits a translucent to transparent gel or clouded gel.

(1-1) Saccharide type biosurfactant "Biosurfactant" is a generic term for substances having surface activity ability and emulsifying ability produced by living things, and not only show excellent surface activity ability and high biodegradability. Because they have various physiological actions, they may exhibit different behaviors and functions from synthetic surfactants.

 Although the component (A) glycobiosurfactant used in the present invention is not particularly limited, MAL (mannosyl alditol lipid) can be mentioned. Examples of MAL include mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), mannosyl ribitol lipid (MRL) and the like, among which MEL is particularly preferred. preferable.

(1-1-1) MEL
The structure of MEL is shown in the general formula (3). In the general formula (3), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different. MEL is classified into four types of MEL-A, MEL-B, MEL-C and MELD based on the presence or absence of acetyl groups at the 4- and 6-positions of mannose.

  Specifically, in MEL-A, in the general formula (3), both of the substituents R2 and R3 are acetyl groups. In MEL-B, in the general formula (3), the substituent R2 is an acetyl group, and the substituent R3 is hydrogen. In MEL-C, in the general formula (3), the substituent R2 is hydrogen and the substituent R3 is an acetyl group. In MEL-D, in the general formula (3), both of the substituents R2 and R3 are hydrogen.

  The carbon number of the substituent R1 in the above MEL-A to MEL-D is the carbon number of the fatty acid constituting the triglyceride that is the oil to be contained in the MEL production medium, and the degree of assimilation of the fatty acid of the MEL producing bacteria used It changes with In addition, when the triglyceride has an unsaturated fatty acid residue as described above, it is also possible to include an unsaturated fatty acid residue as a substituent R1 unless the MEL producing bacteria assimilates to the double bond portion of the unsaturated fatty acid. is there. As apparent from the above description, the obtained MEL is usually in the form of a mixture of compounds different in the fatty acid residue part of the substituent R1.

  The emulsion composition of the present invention contains MEL having the structure shown in the general formula (4) or the general formula (5). In the general formula (4), the substituent R 1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different, and the substituent R 2 is a hydrogen or an acetyl group which may be the same or different The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms. In the general formula (5), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different, and the substituent R2 is a hydrogen or an acetyl group which may be the same or different The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms.

  Substituent R1 in General formula (4) and General formula (5) is a C4-C24 aliphatic acyl group which may be same or different. The number of carbon atoms of the substituent R1 is not particularly limited as long as it is within the above range, but 8 to 14 are more preferable.

  Further, the substituent R1 in the general formula (4) and the general formula (5) may be a saturated aliphatic acyl group or an unsaturated aliphatic acyl group, and is not particularly limited. When having a unsaturated bond, for example, it may have a plurality of double bonds. The carbon chain may be linear or branched. Moreover, in the case of an oxygen atom containing hydrocarbon group, the number and position of the oxygen atom contained are not specifically limited.

  The fatty acid introduced into the erythritol part of MEL may be a long chain hydrocarbon monovalent carboxylic acid. Moreover, it may be saturated fatty acid or unsaturated fatty acid. In the case of unsaturated fatty acids, they may have multiple double bonds. The carbon chain may be linear or branched. Furthermore, fatty acid derivatives that are derivatives of fatty acids may be used in the present invention, or mixtures of fatty acids and fatty acid derivatives may be used in the present invention. The fatty acid or fatty acid derivative to be introduced into the erythritol part of MEL is preferably derived from oils, higher fatty acids and synthetic esters.

(1-1-2) MAL other than MEL
The structure of MAL (MML, MAraL, MRL, MSL) other than MEL is shown in the general formula (6) (wherein the substituent R2 is the same or different hydrogen or acetyl group which may be different). In these MALs, mannitol, arabitol, ribitol and sorbitol are added in place of erythritol as sugar alcohol (alditol) (n = 4: mannitol, sorbitol, n = 2: arabitol, ribitol). Corresponding to the general formula (6), MAL has 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, and more preferably 6 to 14 carbon atoms in the 2 or 3 position of mannose. Having an alkanoyl group having a chain or a branch (in which, the substituent R 2 may be the same or different hydrogen or an acetyl group)

(Wherein the substituent R 1 may be the same or different, and may be saturated or unsaturated linear or branched C 2-20, preferably C 4-18, more preferably C 6-14 carbon atoms. It has an alkanoyl group, and in the formula, the substituent R 2 may be the same or different hydrogen or acetyl group, preferably a compound in which both of the substituents R 2 are acetyl groups)

  The biosurfactant preferably used in the present invention is MEL-B or MEL-C, more preferably MEL-B having a structure represented by the general formula (7) or (8). More preferably, it is MEL-B which has a structure shown by General formula (8).

(In the general formula (7) and the general formula (8), the substituent R 1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different)

  The biosurfactant may be used alone or in combination of two or more.

  The present invention provides an emulsion composition containing MEL as a main emulsifier, but the compounding amount of MEL may be added as long as the effect of MEL is not impaired as the emulsion composition, and the emulsion composition is stable. From the viewpoint of properties, the lower limit is usually preferably 0.1% by mass, more preferably 0.5% by mass, still more preferably 1.0% by mass, and still more preferably 2.0% by mass. The upper limit is preferably 30% by mass, more preferably 20% by mass, still more preferably 10% by mass, still more preferably 5.0% by mass, and still more preferably 4.0% by mass. Here, the use form of the MEL to be added to the composition of the present invention is optional. For example, MEL may be used as an extract from a culture solution as it is, or may be suspended in purified high-purity products or water, or dissolved in a solvent.

(1-3) Oil component The component (B) of the present invention is not particularly limited, and examples thereof include an oil component compatible with skin which can be used for cosmetics. The oily component used in the present invention may be not only a compound but also a natural oily component. Examples of the oil component suitable for the present invention include macadamia nut oil, avocado oil, olive oil, rapeseed oil, castor oil, safflower oil, cottonseed oil, jojoba oil, coconut oil, palm oil, liquid lanolin, hardened coconut oil, hardened oil, Oils and waxes such as hydrogenated castor oil, beeswax, candelilla wax, carnauba wax, lanolin, reduced lanolin, hard lanolin, jojoba wax, liquid paraffin, squalane, paraffin, ceresin, vaseline, hydrocarbons such as microcrystallin wax, oleic acid Fatty acids such as isostearic acid, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid and undecylenic acid, cetyl alcohol, stearyl alcohol, isostearyl alcohol, behenyl alcohol, octyl dodecanol, myristi Alcohol, higher alcohols such as cetostearyl alcohol, cetyl isooctanoate, isopropyl myristate, hexyl decyl isostearate, diisopropyl adipate, di 2-ethylhexyl sebacate, cetyl lactate, diisostearyl malate, di 2-ethyl hexanoic acid Synthesis of ethylene glycol, neopentyl glycol dicaprate, glyceryl diisostearate, glyceryl tri-2-ethylhexanoate, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra 2-ethylhexanoate, etc. Linear polysiloxanes such as ester oils, dimethylpolysiloxane, methylphenylpolysiloxane, diphenylpolysiloxane, octamethylcyclotetracycle Oil agents such as silicone oils such as cyclic polysiloxanes such as xanthan, decamethylcyclopentasiloxane and dodecamethylcyclohexanesiloxane, amino-modified polysiloxanes, polyether-modified polysiloxanes, alkyl-modified polysiloxanes, fluorine-modified polysiloxanes and other modified polysiloxanes One or more oil-based components selected from the group consisting of classes can be employed.

  The content of the oil component of the component (B) of the present invention is not particularly limited, but from the viewpoint of the stability of the emulsion composition, the lower limit is usually preferably 50.0% by mass, more preferably 60. It is 0% by mass, more preferably 70.0% by mass. On the other hand, the upper limit is preferably 90.0% by mass, more preferably 80.0% by mass.

(1-4) Water The component (C) water used in the present invention is not particularly limited as long as it is clean to a degree suitable for the intended use of the emulsion composition. For example, plant-derived water vapor distilled water such as deionized water, distilled water, purified water, deep ocean water, hot spring water, rose water, lavender water etc. may be mentioned, and one or more of these may be used in combination It can be used.

  The content of the component (C) of the present component is not particularly limited, and may be appropriately determined depending on the other components. That is, it becomes the remainder except the sum total of mass% of (A), (B), (D) and other additives described separately. From the viewpoint of the stability of the emulsion composition, the lower limit is usually preferably 0.01% by mass, more preferably 0.1% by mass, and still more preferably 1.0% by mass. On the other hand, the upper limit is preferably 30% by mass, more preferably 10% by mass, and still more preferably 7% by mass.

(1-5) Glycyrrhizinate Salt (D) Glycyrrhizinate used in the present invention includes dipotassium glycyrrhizinate, sodium glycyrrhizinate, monoammonium glycyrrhizinate and the like, but the type of salt is not particularly limited. These glycyrrhizinate can be used alone or in combination of two or more. Alternatively, glycyrrhizinic acid may be neutralized with a suitable base to form a salt. These may select the component generally used for cosmetics.

  The component (D) glycyrrhizinate of the present invention has a very high dissolving power of the component (A) and is excellent in the effect as a component for dissolving and dispersing the component (A) in water. In order to obtain an emulsion composition containing a high concentration of an oily component according to the present invention, the component (A) needs to be efficiently dissolved in a solvent. The formulation of component (D) is very important in that it helps the efficient dissolution of component (A). Usually, the preferable lower limit is 0.01% by mass, more preferably 0.1% by mass, and still more preferably 0.5% by mass. On the other hand, the upper limit is preferably 10.0% by mass, more preferably 5.0% by mass, and still more preferably 2.0% by mass.

  The emulsion composition in the present invention is not particularly limited in the compounding ratio of the component (A) to the component (D), but can preferably be obtained in 10: 1 to 1: 5 (mass ratio), and the stability And from the viewpoint of usability, more preferably 5: 1 to 1: 1 (mass ratio).

  The effect of improving the stability and usability is expected by improving the blending ratio of the component (D) to the component (A) as the content of the component (A) decreases. Alternatively, as the content of the component (A) decreases, an effect of improving the stability and usability can be expected by improving the content of the component (E).

(1-6) Polyhydric alcohol The emulsion composition of the present invention may further contain component (E) a polyhydric alcohol. The polyhydric alcohol used in the present invention is a divalent or higher polyhydric alcohol having two or more hydroxyl groups in the same molecule, and examples thereof include dihydric alcohol (glycol) and sugar alcohol. For example, one or more selected from the group consisting of glycerin, diglycerin, propanediol, propylene glycol, dipropylene glycol, butylene glycol, pentylene glycol, hexylene glycol, polyethylene glycol, sorbitol, maltitol, sucrose, erythritol and xylitol etc. The following compounds can be adopted.

  The content in the case of containing the component (E) polyhydric alcohol in the emulsion composition is not particularly limited, but from the viewpoint of the stability of the emulsion composition, the preferred lower limit is usually 5.0% by mass. More preferably, it is 10.0 mass%. On the other hand, the upper limit is preferably 30.0% by mass, more preferably 20.0% by mass.

(1-7) Other Components The emulsion composition of the present invention may contain, in addition to the components as described above, various components used for ordinary cosmetics, quasi-drugs, medicines, etc. Of course, for example, moisturizers, thickeners, medicinal ingredients, preservatives, pigments, powders, pH adjusters, ultraviolet absorbers, antioxidants, perfumes and the like can be appropriately blended.

  Any moisturizing agent may be used as long as it is a component generally used in cosmetics, and examples thereof include pyrrolidone carboxylic acid, lactic acid, sodium lactate, amino acid, sodium hyaluronate, sodium chondroitin sulfate and the like. Be The addition of these moisturizing agents is expected to improve the feeling of use and impart moisture to the emulsified composition of the present invention.

  As the above-mentioned thickener, any component generally used in cosmetics may be used, such as guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, Curdlan, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, methyl hydroxypropyl cellulose, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, locust bean gum, succino glucan, caroninic acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, Polyvinyl pyrrolidone, carboxy vinyl polymer, alkyl-modified carboxy vinyl polymer, sodium polyacrylate, bentonite and the like can be mentioned. These thickeners are expected to improve the stability of the emulsion composition of the present invention.

  Any medicinal component may be used as long as it is a component generally used in cosmetics. For example, vitamin A or a derivative thereof, vitamin B6 or a derivative thereof, vitamin B2 or a derivative thereof, vitamin B12, vitamin B15 Or vitamin Bs such as α-tocopherol, β-tocopherol, γ-tocopherol, vitamin E acetate, vitamin E, vitamin D, vitamin H, pantothenic acid, panthetin, pyrroloquinoline quinone etc. Various animal and plant extracts and the like can be mentioned. The addition of these pharmaceutically active ingredients is expected to impart an antioxidative effect and an antiaging effect to the emulsified composition of the present invention.

  As the above-mentioned preservative, any component generally used in cosmetics may be used, for example, parabens such as methyl paraben, ethyl paraben, propyl paraben, butyl paraben, phenoxyethanol, ethylhexyl glycerin etc. It can be mentioned. The addition of these preservatives is expected to improve the preservative properties of the emulsified composition of the present invention.

  As the above-mentioned pH adjusting agent, any component may be used as long as it is a component generally used in cosmetics. For example, citric acid, sodium citrate, lactic acid, sodium lactate, sodium hydroxide, sodium hydroxide K, etc. Can be mentioned. The addition of these pH adjusting agents is expected to improve the stability and the like of the emulsified composition of the present invention.

  By adding various components as described above to the emulsion composition of the present invention, depending on the components, effects such as moisturizing action, cell activating action, anti-aging action, hair growth action and rough skin improving action are imparted. It can be expected to do.

  The method of preparing the emulsion composition of the present invention is a method of mixing and dissolving a sugar type biosurfactant compound, water and glycyrrhizinate, and gradually adding an oil component while vigorously stirring to obtain an emulsion composition. It is preferable for the stabilization of When a polyhydric alcohol is used, the glycobiosurfactant compound, water and glycyrrhizinate are mixed and dissolved, and then the polyhydric alcohol is added and mixed again, and then the oily component is gradually added while vigorously stirring. The method of obtaining an emulsified composition is preferable. These methods may be combined with mechanical emulsification in which stirring, mixing, and emulsification are carried out using a propeller mixer, a paddle mixer, a disperser mixer, a homogenizer or the like.

  The appearance of the emulsified composition obtained in the present invention may be transparent, slightly turbid or cloudy, and the physical properties may be liquid, gel, cream or solid.

  The emulsion composition obtained by the present invention may be any of W / O type, O / W type or O / D type emulsion composition, but the emulsion composition prepared by the above method is preferably O /. 1 shows a D-type emulsion.

  The emulsion composition obtained as described above can be diluted with water to obtain an emulsion composition different from the above. An emulsion composition which is an O / W emulsion can be obtained by diluting the emulsion composition which is an O / D emulsion with water. The dilution solvent is not particularly limited to water alone, and water-soluble components generally used in cosmetics may be mixed. That is, there is no problem even if one or two or more mixed water-soluble components are used as the dilution solvent.

  The appearance of the emulsified composition obtained by dilution may be slightly turbid or cloudy, and the physical properties may be liquid, gel, cream or solid.

  From the viewpoint of stability and usability of the emulsion composition after dilution, it is desirable that the appearance of the emulsion composition before dilution is in the form of a clear gel, and by diluting the emulsion composition in this state, a fine emulsion is obtained. It is expected to obtain a milky white emulsion composition consisting of

  The emulsion composition obtained in the present invention can be applied to general cosmetic preparations. Specifically, shampoos, conditioners, hair cosmetics such as treatments, facial cleansers, cleansing cosmetics, sunscreen cosmetics, packs, massage cosmetics, skin cosmetics such as lotions, emulsions, creams, eyeliners, mascaras , Lipstick, foundation and other makeup cosmetics, bath cosmetics and the like.

  EXAMPLES The present invention will be specifically described by way of examples, but the present invention is not limited to the examples.

(Production example)
Production Example 1 Production of MEL-B Pseudozyma tsukubaensis (NBRC 1940) was aerated and stirred for 48 hours at a culture temperature of 26 ° C. in a YM medium using a 500 ml volume Sakaguchi flask. The obtained solution is used as a seed culture solution. The obtained seed culture solution was aerated and cultured in YM medium (containing 5% olive oil) at a culture temperature of 26 ° C. for 7 days using a 10 L jar fermenter. An equal volume of ethyl acetate was added to the culture solution, and the mixture was stirred and distributed. An appropriate amount of anhydrous Na 2 SO 4 was added to the ethyl acetate layer, and the mixture was allowed to stand for 30 minutes, and the mixture was heated and concentrated to obtain crude MEL-B. The obtained crude MEL-B was subjected to chloroform: acetone = 1: 0, chloroform: acetone = 9: 1, chloroform: acetone 1: 1, chloroform: acetone = 3: 7, chloroform: acetone using a silica gel column. Elution occurred at 0: 1. The MEL-B fraction was fractionated and concentrated to obtain purified MEL-B.

Production Example 2 Production of MEL-C Pseudozyma hubeiensis KM-59 strain (FERM-20987) was aerated and stirred for 48 hours at a culture temperature of 26 ° C. in a YM medium using a 500 ml volume Sakaguchi flask. The obtained solution is used as a seed culture solution. The obtained seed culture solution was aerated and cultured in YM medium (containing 5% olive oil) at a culture temperature of 26 ° C. for 7 days using a 10 L jar fermenter. An equal volume of ethyl acetate was added to the culture solution, and the mixture was stirred and distributed. An appropriate amount of anhydrous Na 2 SO 4 was added to the ethyl acetate layer, and the mixture was allowed to stand for 30 minutes, and the mixture was heated and concentrated to obtain crude MEL-C. The obtained crude MEL-C was subjected to chloroform: acetone = 1: 0, chloroform: acetone = 9: 1, chloroform: acetone 1: 1, chloroform: acetone = 3: 7, chloroform: acetone using a silica gel column. Elution occurred at 0: 1. The MEL-C fraction was fractionated and concentrated to obtain purified MEL-C.

(Example)
Using the glycotype biosurfactant compounds obtained in the above-mentioned Production Examples 1 and 2, the emulsion compositions of Examples 1 to 15 and Comparative Examples 1 to 5 with the components and blending ratios shown in Tables 1, 2 and 3 Prepared.

(Conditions for preparing an emulsified composition)
MEL-B, purified water, and dipotassium glycyrrhizinate are heated and dissolved at 50 to 60 ° C. and mixed according to the mixing ratio shown in Tables 1 and 2 below, and a polyhydric alcohol (glycerin, 1, 3, or 2 as necessary) -After adding propanediol or 1,3-butylene glycol and mixing uniformly again, while stirring with a propeller mixer (1,000 rpm), various oily components (squalane, dimethylpolysiloxane, olive oil or tri (capryl / An emulsion composition was prepared by gradually charging capric acid) glyceryl).

  Similarly, MEL-C, purified water, and dipotassium glycyrrhizinate were heated and dissolved at 50 to 60 ° C. and mixed according to the mixing ratio shown in Table 3, and glycerin which is a polyhydric alcohol was added and uniformly mixed again. Thereafter, while stirring with a propeller mixer (1,000 rpm), an oily component (squalane) was gradually added to prepare an emulsified composition.

(Evaluation method)
With respect to the obtained emulsified composition, the properties of the emulsified composition immediately after production and the stability when stored for 6 months in a thermostat at 25 ° C. are also described in Tables 1 and 2. The properties were evaluated by visually observing the state of emulsification or separation.
:: high in transparency, maintaining gel-like ○: semitransparent to slightly turbid, but maintaining gel-like ×: separated, no gel-like

Moreover, the evaluation by the property was performed also about the emulsion composition obtained by diluting this emulsion composition 10 times. 10% by mass of the emulsified composition is added to an aqueous solution containing 0.2% by mass (final concentration) of carboxyvinyl polymer, and after mixing by a standard method, it is stored in a thermostat at 25 ° C. for 6 months The stability is also described in Table 1 and Table 2 as "Properties of Dilution Solution (25 ° C 6 months)". The properties were evaluated by visually observing the state of emulsification or separation.
:: A milky white well maintained emulsified state ○: A milky white to white emulsified state maintained ×: separation

  In Comparative Examples 1 to 5, when any component of MEL-B, glycyrrhizinate, oil component or water is not blended, or when the content of oil component (squalane) is relatively low at 40% by mass, The mixture did not form a gel and separated immediately after preparation.

  On the other hand, the stable gel-like emulsion composition was able to be obtained by mix | blending MEL-B, a glycyrrhizinate, an oil-based component, and water as an essential component in Examples 1-13. Moreover, also in Examples 14 and 15, a stable gel-like emulsified composition could be obtained by blending MEL-C, glycyrrhizinate, an oil component and water as essential components.

  In the examples, squaraine, dimethylpolysiloxane, olive oil, and glyceryl tri (capryl / capric acid) were used as an oil component to obtain the emulsion composition of the present invention, but containing components similar to these oil components. With regard to the oily component, it is also considered possible to obtain the emulsion composition of the present invention.

  According to the present invention, it is possible to prepare an emulsion composition containing MEL which is a sugar-type biosurfactant compound as a main emulsifier. The emulsion composition of the present invention is capable of emulsifying various types of oily components. It is expected that this emulsion composition and its formulation make it possible to prepare a very useful cosmetic preparation and to greatly contribute to the industry.

Claims (7)

(A) A sugar type biosurfactant, (B) an oily component, (C) water, (D) glycyrrhizinate , and (E) a polyhydric alcohol, and the blending amount of the (B) oily component is 50.0 The emulsion composition which is mass% or more, and a compounding ratio of (A) sugar type biosurfactant and (D) glycyrrhizinate is 5: 1 to 1: 1 (mass ratio), and is an O / D type emulsion . (A) The sugar type biosurfactant is selected from the group consisting of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL) The emulsion composition according to claim 1, which is one or more selected compounds. The emulsion composition according to claim 2 , wherein (A) glycoform biosurfactant is mannosyl erythritol lipid B (MELB) and / or mannosyl erythritol lipid C (MEL-C). The O / W type emulsion composition which consists of what diluted the emulsion composition as described in any one of Claims 1-3 with water. (A) A sugar type biosurfactant, (C) water and (D) glycyrrhizinate are mixed and dissolved, and then an O / D type emulsion is added so as to be 50.0% by mass or more of an oily component while stirring (B) A method of producing an emulsion composition. (A) A sugar type biosurfactant, (C) water and (D) glycyrrhizinate are mixed and dissolved, and further (E) a polyhydric alcohol is dissolved, and then with stirring, 50.0% by mass of an oily component (B) The manufacturing method of the emulsion composition of Claim 5 added so that it may become above. Further, the production method of the emulsion composition according to claim 5 or 6 comprising the step of diluting with water.