JP6586854B2 - Emulsified composition - Google Patents

Description

  The present invention relates to an emulsified composition containing a sugar type biosurfactant compound.

  The skin moisturizing function functions by binding intercellular lipids to moisture, and a skin care material having a moisturizing performance close to the original skin function has been required. In recent years, consumers are increasingly interested in natural products because of concerns about safety, and materials derived from natural products, not synthetic products, are required in the skin care field. For example, materials obtained by extraction from natural products such as lecithin and saponin are used in the skin care field. In addition, in recent years, research and development for using a fermented product produced by microorganisms as a skin care material has been actively conducted.

  Examples of the skin care material produced by the above-described microorganisms include biosurfactants that are amphiphilic substances having both hydrophilic groups and lipophilic groups and have surface-active ability. Biosurfactants, which are microorganism-derived surfactants, are being studied as environmentally advanced surfactants that are highly safe and have a low environmental impact and excellent biodegradability.

At present, biosurfactants are classified into five types: sugar type, acyl peptide type, phospholipid type, fatty acid type, and polymer compound type. Among these, the sugar type biosurfactant is most well studied, and the bacteria And many types of substances produced by yeast have been reported. As such a sugar-type biosurfactant, for example, mannosyl erythritol lipid (hereinafter sometimes referred to as “MEL”) has recently been used in the skin care field.

  MEL has 4-O-β-D-mannopyranosyl-meso-erythritol structure and 1-O-β-D-mannopyranosyl-form as shown in the following general formula (1) as optical isomers of erythritol having a sugar skeleton. A meso-erythritol structure (the following general formula (2)) exists.

MEL having a conventional 4-O-β-D-mannopyranosyl-meso-erythritol structure has been reported to have various physiological activities including antibacterial properties, antitumor properties, glycoprotein binding ability (non- Patent Document 1). In addition, this conventional MEL exhibits extremely unique self-assembly characteristics, and not only a slight difference in molecular structure greatly affects the formation of self-assemblies, but also dilute solutions (6 3 × 10 ⁻² wt% or less) (non-patent document 2). Furthermore, a liquid crystal emulsification technique (Patent Document 1) using a conventional MEL bicontinuous sponge structure is also reported.

However, although MEL is a surfactant derived from microorganisms, it is not considered to have a high ability to emulsify oil by itself, and can only be used as an emulsification aid in the preparation of an emulsion composition. (Patent Document 2). That is, it is difficult to prepare an emulsified composition containing MEL as a main emulsifier, and no such report has been made heretofore.

JP 2007-181789 A WO2011 / 149007

Dai Kitamoto “Oreoscience” (Japan), Japan Oil Chemists' Society, Volume 3, p663-672 (2003). Tee. T. Imura, N. Ota, K. Inoue, N. Goat (N. Yagi), H. H. Negishi, H. H. Yanagishita, Di. D. Kitamoto “Chemistry European Journal” (USA), Wiley, Vol. 12, p 2434-2440 (2006).

It is possible to widely use sugar-type biosurfactant compounds such as MEL, which have high biodegradability, low toxicity, environmental friendliness, and new physiological functions in the food industry, cosmetic industry, pharmaceutical industry, chemical industry, environmental fields, etc. It is desired.

Sugar-type biosurfactants such as MEL are known to have surface-active ability because they have both lipophilic and hydrophilic regions in the molecule, and are expected to be used as emulsifiers derived from new natural products. . However, since MEL and the like have extremely low solubility in water and oil, it has been difficult to prepare a stable emulsion composition using MEL or the like as a main emulsifier.

Therefore, an object of the present invention is to provide a stable emulsified composition and a prescription thereof using a sugar-type biosurfactant such as MEL as a main emulsifier.

As a result of intensive studies, the present inventors have found that the above problems can be solved by the following means, and have reached the present invention.
That is, this invention consists of the following structures.
[1] It contains (A) a sugar-type biosurfactant, (B) an oil component, (C) water, and (D) an anionic surfactant, and the blending amount of (B) oil component is 50.0% by mass or more. Yes, (A) Sugar type biosurfactant and (D) Anionic surfactant are 10: 1 to 1: 5 (mass ratio), and the emulsion composition characterized by the above-mentioned.
[2] The emulsion composition according to [1], wherein the emulsion composition is an O / D type emulsion.
[3] (A) The sugar type biosurfactant is composed of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL). The emulsified composition according to [1] or [2], which is one or more compounds selected from the group.
[4] The (A) sugar-type biosurfactant is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B) and / or mannosyl erythritol lipid C (MEL-C). The emulsion composition according to [3].
[5] One type of anionic surfactant selected from the group consisting of fatty acid soaps, carboxylic acid anionic surfactants, phosphoric acid anionic surfactants, sulfonic acid anionic surfactants and sulfuric acid anionic surfactants Or the emulsion composition in any one of [1]-[4] which is 2 or more types of surfactant.
[6] The emulsion composition according to any one of [1] to [5], further comprising (E) a polyhydric alcohol.
[7] An O / W emulsion composition comprising the emulsion composition according to any one of [1] to [6] diluted with water.
[8] (A) Sugar type biosurfactant, (C) water and (D) anionic surfactant are mixed and dissolved, and then (B) oily component is added to 50.0% by mass or more with stirring. A method for producing an emulsified composition.
[9] (A) Sugar type biosurfactant, (C) water and (D) anionic surfactant are mixed and dissolved, and (E) polyhydric alcohol is dissolved, and then (B) oily component is added to 50 with stirring. The method for producing an emulsified composition according to [8], which is added so as to be not less than 0.0% by mass.
[10] The method for producing an emulsified composition according to [8] or [9], further comprising a step of diluting with water.

  According to the present invention, a stable emulsion composition can be prepared using a sugar-type biosurfactant such as MEL as a main emulsifier. In addition, the emulsified composition is not only stable, but also can emulsify various types of oily components.

  The emulsified composition and the formulation thereof provided by the present invention have effects such as a moisturizing action, a cell activation action, an anti-aging action, a hair growth action, and a rough skin improving action. Moreover, the improvement of the effect is anticipated by adding a various compounding component. This emulsified composition can be provided as cosmetics, quasi drugs, medical supplies, hygiene products, and pharmaceuticals.

Further, since the emulsion composition of the present invention has a cleansing property, a moisturizing effect, a cell activation effect, an anti-aging effect, a hair growth effect, a rough skin improving effect and the like, it is expected to be used as a blending component of a cleaning cosmetic. .

  Since biosurfactant is derived from living organisms, it is excellent in safety, and the composition according to the present invention has an effect that it can sufficiently withstand long-term use. Furthermore, since biosurfactants can be produced by culturing microorganisms, raw material costs are low, and mass production is possible. Therefore, the composition according to the present invention can be provided at a low price.

(1) Emulsion composition One embodiment of the present invention is an emulsion composition containing a sugar-type biosurfactant, an oil component, water, and an anionic surfactant as essential components.

  The form of the “emulsified composition” of the present invention is not particularly limited as long as it is a dispersion system containing a sugar-type biosurfactant, an oily component, water, and an anionic surfactant as essential components, but preferably a surfactant phase ( D phase) is an O / D emulsion having a continuous phase and an oil phase as a dispersed phase. The emulsified composition of the present invention preferably exhibits a translucent to transparent gel or cloudy gel.

(1-1) Sugar type biosurfactant “Biosurfactant” is a general term for substances having surface-active ability and emulsifying ability produced by living organisms, and not only exhibits excellent surface-active ability and high biodegradability. Since it has various physiological actions, it may develop behavior / function different from those of synthetic surfactants.

The component (A) sugar-type biosurfactant used in the present invention is not particularly limited, and examples thereof include MAL (mannosyl alditol lipid). Examples of MAL include mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL), and mannosyl ribitol lipid (MRL). preferable.

(1-1-1) MEL
The structure of MEL is shown in general formula (3). In general formula (3), substituent R1 is a C4-C24 aliphatic acyl group which may be the same or different. MEL is classified into four types, MEL-A, MEL-B, MEL-C, and MEL-D, based on the presence or absence of acetyl groups at the 4th and 6th positions of mannose.

Specifically, in MEL-A, in the general formula (3), the substituents R2 and R3 are both acetyl groups. In MEL-B, in the general formula (3), the substituent R2 is an acetyl group, and the substituent R3 is hydrogen. In MEL-C, in general formula (3), substituent R2 is hydrogen, and substituent R3 is an acetyl group. In MEL-D, in general formula (3), substituents R2 and R3 are both hydrogen.

The carbon number of the substituent R1 in the above MEL-A to MEL-D is the carbon number of the fatty acid constituting the triglyceride that is an oil or fat to be contained in the MEL production medium, and the degree of utilization of the fatty acid of the MEL-producing bacterium to be used It depends on. In addition, when the triglyceride has an unsaturated fatty acid residue, it is possible to include an unsaturated fatty acid residue as the substituent R1 unless the MEL-producing bacterium assimilates up to the double bond portion of the unsaturated fatty acid. is there. As apparent from the above description, the obtained MEL is usually in the form of a mixture of compounds having different fatty acid residue portions of the substituent R1.

The emulsified composition of the present invention contains MEL having the structure represented by the general formula (4) or the general formula (5). In the general formula (4), the substituent R1 is the same or different aliphatic acyl group having 4 to 24 carbon atoms, and the substituent R2 is the same or different hydrogen or acetyl group. The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms. In the general formula (5), the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different, and the substituent R2 is a hydrogen or acetyl group which may be the same or different. The substituent R3 is hydrogen or an aliphatic acyl group having 2 to 24 carbon atoms.

The substituent R1 in the general formula (4) and the general formula (5) is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different. The number of carbon atoms of the substituent R1 is not particularly limited as long as it is within the above range, but it is more preferably 8 to 14.

The substituent R1 in the general formulas (4) and (5) may be a saturated aliphatic acyl group or an unsaturated aliphatic acyl group, and is not particularly limited. When it has an unsaturated bond, you may have a some double bond, for example. The carbon chain may be linear or branched. In the case of an oxygen atom-containing hydrocarbon group, the number and position of oxygen atoms contained are not particularly limited.

The fatty acid introduced into the erythritol part of MEL may be a long-chain hydrocarbon monovalent carboxylic acid. Further, it may be a saturated fatty acid or an unsaturated fatty acid. In the case of an unsaturated fatty acid, it may have a plurality of double bonds. The carbon chain may be linear or branched. Furthermore, a fatty acid derivative that is a derivative of a fatty acid may be used in the present invention, or a mixture of a fatty acid and a fatty acid derivative may be used in the present invention. The fatty acid or fatty acid derivative introduced into the erythritol part of MEL is preferably derived from oils, higher fatty acids, and synthetic esters.

(1-1-2) MAL other than MEL
The structure of MAL (MML, MARaL, MRL, MSL) other than MEL is shown in the general formula (6) (wherein the substituents R2 are the same or different hydrogen or acetyl groups). In these MALs, mannitol, arabitol, ribitol, and sorbitol are added as sugar alcohol (alditol) instead of erythritol (n = 4: mannitol, sorbitol, n = 2: arabitol, ribitol). According to the general formula (6), MAL is a saturated or unsaturated straight chain having 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, more preferably 6 to 14 carbon atoms at the 2nd and 3rd positions of mannose. It has an alkanoyl group having a chain or a branch (wherein the substituent R2 is a hydrogen atom or an acetyl group which may be the same or different).

(In the formula, the substituents R1 may be the same or different and each has a saturated or unsaturated straight or branched chain having 2 to 20 carbon atoms, preferably 4 to 18 carbon atoms, more preferably 6 to 14 carbon atoms. (In the formula, the substituent R2 is a hydrogen atom or an acetyl group, which may be the same or different, and is preferably a compound in which both of the substituents R2 are acetyl groups.)

  The biosurfactant preferably used in the present invention is MEL-A, MEL-B or MEL-C, more preferably MEL-B having the structure represented by the general formula (7) or the general formula (8). . More preferably, it is MEL-B which has a structure shown by General formula (8).

(In the general formula (7) and the general formula (8), the substituent R1 is the same or different aliphatic acyl group having 4 to 24 carbon atoms)

  In addition, although a biosurfactant may be used independently, 2 or more types of biosurfactants can also be used together.

The present invention provides an emulsified composition containing MEL as a main emulsifier, but the blending amount of MEL may be added within a range that does not impair the action of MEL as an emulsified composition. From the viewpoint of properties, the usually preferred lower limit is 0.1% by mass, more preferably 0.2% by mass, still more preferably 0.5% by mass, and further preferably 1.0% by mass. On the other hand, the preferable upper limit is 20% by mass, more preferably 10% by mass, further preferably 5.0% by mass, and further preferably 3.0% by mass. Here, the usage form of the MEL added to the composition of the present invention is arbitrary. For example, MEL may be used as it is as an extract from the culture solution, or after being purified, purified or suspended in water, or dissolved in a solvent.

(1-3) Oily component Although the component (B) oily component of this invention is not specifically limited, For example, the oily component which is easy to adapt to the skin which can be used for cosmetics is mentioned. The oil component used in the present invention may be not only a compound but also a natural oil component. Suitable oily components for this invention include, for example, macadamia nut oil, avocado oil, olive oil, rapeseed oil, castor oil, safflower oil, cottonseed oil, jojoba oil, coconut oil, palm oil, liquid lanolin, hardened coconut oil, hardened oil, Oils and waxes such as hydrogenated castor oil, beeswax, candelilla wax, carnauba wax, lanolin, reduced lanolin, hard lanolin, jojoba wax, hydrocarbons such as liquid paraffin, squalane, paraffin, ceresin, petrolatum, microcrystalline wax, oleic acid , Higher fatty acids such as isostearic acid, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, undecylenic acid, cetyl alcohol, stearyl alcohol, isostearyl alcohol, behenyl alcohol, octyldodecanol, myristic Alcohol, higher alcohols such as cetostearyl alcohol, cetyl isooctanoate, isopropyl myristate, hexyl decyl isostearate, diisopropyl adipate, di-2-ethylhexyl sebacate, cetyl lactate, diisostearyl malate, di-2-ethyl Ethylene glycol hexanoate, neopentyl glycol dicaprate, glycerin di-2-heptylundecanoate, glycerin tri-2-ethylhexanoate, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, tetra-2 -Synthetic ester oils such as ethylhexanoic acid pentane erythritol, chain polysiloxanes such as dimethylpolysiloxane, methylphenylpolysiloxane, diphenylpolysiloxane, Silicone oils such as cyclic polysiloxanes such as methylcyclotetrasiloxane, decamethylcyclopentasiloxane, dodecamethylcyclohexanesiloxane, amino-modified polysiloxanes, polyether-modified polysiloxanes, alkyl-modified polysiloxanes, modified polysiloxanes such as fluorine-modified polysiloxanes One or more oily components selected from the group consisting of oil agents and the like can be employed.

Although content of the component (B) oily component of this invention is not specifically limited, From a viewpoint of the stability of an emulsion composition, a normally preferable minimum is 50.0 mass%, More preferably, 60. It is 0 mass%, More preferably, it is 70.0 mass%. On the other hand, a preferable upper limit is 90.0% by mass, and more preferably 80.0% by mass.

(1-4) Water The component (C) water used in the present invention is not particularly limited as long as it is clean enough to suit the intended use of the emulsion composition. For example, deionized water, distilled water, purified water, deep ocean water, hot spring water, rose water, lavender water and other plant-derived steam distilled water, etc. can be mentioned, and one or more of these can be combined Can be used.

The content of component (C) of this component is not particularly limited and can be appropriately determined depending on other components. That is, it is the remainder excluding the sum of the mass% of (A), (B), (D) and other additives, which are described separately. From the viewpoint of the stability of the emulsion composition, the usually preferred lower limit is 0.01% by mass, more preferably 0.1% by mass, and further preferably 1.0% by mass. On the other hand, a preferable upper limit is 20% by mass, more preferably 10% by mass, and further preferably 5% by mass.

(1-5) Anionic Surfactant (D) Anionic surfactant used in the present invention is not particularly limited, but fatty acid soap, alkyl ether carboxylate, fatty acid amide ether carboxylate, acyl lactic acid Carboxylic acid anion surfactants such as salts, N-acyl amino acid salts, phosphate anions such as alkyl phosphates, polyoxyethylene alkyl ether phosphates, alkylaryl ether phosphates, fatty acid amide ether phosphates Surfactant, alkane sulfonate, α-olefin sulfonate, α-sulfo fatty acid methyl ester salt, acyl isethionate, alkyl glycidyl ether sulfonate, alkyl sulfosuccinate, alkyl sulfoacetate, alkyl benzene sulfonate, Alkyl naphthalene sulfonate, N -Sulphonic acid anionic surfactants such as acylmethyl taurate, formalin condensation sulfonate, sulfuric acid such as alkyl sulfate, alkyl ether sulfate, alkyl aryl ether sulfate, fatty acid alkanolamide sulfate, fatty acid monoglyceride sulfate And anionic anionic surfactants. These anionic surfactants can be used alone or in combination of two or more. These may select ingredients generally used in cosmetics. Moreover, the usage form of the anionic surfactant to be added to the composition of the present invention is arbitrary. For example, the solid / flake form, the solid / flake form from room temperature to high temperature with a solvent such as water. You may use the aqueous solution dissolved by this, and the commercial item previously melt | dissolved in solvents, such as water.

The component (D) anionic surfactant of the present invention has a very high solubility of the component (A) and is excellent in the effect as a component for dissolving and dispersing the component (A) in water. In order to obtain an emulsified composition containing a high-concentration oily component according to the present invention, the component (A) needs to be efficiently dissolved in a solvent. The blending of component (D) is very important in terms of assisting efficient dissolution of component (A). Usually, a preferable lower limit is 0.01% by mass, more preferably 0.1% by mass, and further preferably 0.5% by mass. On the other hand, a preferable upper limit is 10.0% by mass, more preferably 5.0% by mass, and further preferably 2.0% by mass.

The emulsified composition in the present invention is not particularly limited in the compounding ratio of the component (A) and the component (D), but can preferably be obtained at 10: 1 to 1: 5 (mass ratio), and is stable. From the viewpoint of usability, it is more preferably 5: 1 to 1: 2 (mass ratio).

As the content of the component (A) decreases, an effect of improving stability and usability is expected by improving the blending ratio of the component (D) to the component (A). Or, as the content of the component (A) decreases, the effect of improving the stability and usability is expected by improving the content of the component (E).

(1-6) Polyhydric alcohol The emulsion composition of this invention may contain a component (E) polyhydric alcohol further. The polyhydric alcohol used in the present invention is a dihydric or higher polyhydric alcohol having two or more hydroxyl groups in the same molecule, and examples thereof include dihydric alcohol (glycol) and sugar alcohol. For example, one or more selected from the group consisting of glycerin, diglycerin, propanediol, propylene glycol, dipropylene glycol, butylene glycol, pentylene glycol, hexylene glycol, polyethylene glycol, sorbitol, maltitol, sucrose, erythritol and xylitol These compounds can be employed.

  Although content of the component (E) polyhydric alcohol of this invention is not specifically limited, From a stability viewpoint of an emulsion composition, a normally preferable minimum is 5.0 mass%, More preferably, it is 10 0.0% by mass. On the other hand, the preferable upper limit is 30.0% by mass, and more preferably 20.0% by mass.

(1-7) Other components In addition to the components as described above, the emulsion composition of the present invention may contain various components used in ordinary cosmetics, quasi drugs, pharmaceuticals, and the like. Of course, for example, humectants, thickeners, medicinal ingredients, preservatives, pigments, powders, pH adjusters, ultraviolet absorbers, antioxidants, fragrances and the like can be appropriately blended.

  As the humectant described above, any component that is generally used in cosmetics may be used, and examples thereof include pyrrolidone carboxylic acid, lactic acid, sodium lactate, amino acid, sodium hyaluronate, and chondroitin sulfate. It is done. By adding these humectants, effects such as improved use feeling and moisturizing properties are expected for the emulsion composition of the present invention.

  As the above-mentioned thickener, any of ingredients generally used in cosmetics may be used, such as guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, Curdlan, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, methyl hydroxypropyl cellulose, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, locust bean gum, succinoglucan, caronic acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, Examples include polyvinyl pyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, sodium polyacrylate, bentonite and the like. These thickeners are expected to improve the stability of the emulsion composition of the present invention.

  As the above-mentioned medicinal ingredients, any of ingredients generally used in cosmetics may be used. For example, vitamin A or a derivative thereof, vitamin B6 or a derivative thereof, vitamin B2 or a derivative thereof, vitamin B12, Vitamin B such as vitamin B15 or derivatives thereof, vitamin E such as α-tocopherol, β-tocopherol, γ-tocopherol, vitamin E acetate, vitamin D, vitamin H, pantothenic acid, panthetin, pyrroloquinoline quinone And various animal and plant extracts. Addition of these medicinal ingredients is expected to impart an antioxidant action and an anti-aging effect to the emulsion composition of the present invention.

  As the above-mentioned preservative, any component that is generally used in cosmetics may be used, for example, parabens such as methylparaben, ethylparaben, propylparaben, butylparaben, phenoxyethanol, ethylhexylglycerin and the like. Can be mentioned. By adding these preservatives, an effect of improving the antiseptic property is expected for the emulsion composition of the present invention.

  As the pH adjuster, any component that is generally used in cosmetics may be used. For example, citric acid, Na citrate, lactic acid, Na lactate, Na hydroxide, K hydroxide, etc. Is mentioned. By adding these pH adjusters, effects such as improved stability are expected for the emulsion composition of the present invention.

  By adding various components as described above to the emulsified composition of the present invention, effects such as a moisturizing effect, a cell activation effect, an anti-aging effect, a hair growth effect, and a rough skin improving effect are imparted according to these components. Can be expected to do.

  The method for preparing an emulsified composition of the present invention is a method in which a sugar-type biosurfactant compound, water and an anionic surfactant are mixed and dissolved, and an oily component is gradually added while stirring vigorously to obtain an emulsified composition. It is preferable for stabilizing the product. When polyhydric alcohol is used, the sugar type biosurfactant compound, water and anionic surfactant are mixed and dissolved, then the polyhydric alcohol is added and mixed and dissolved again, and then the oily component is gradually mixed with strong stirring. A method for obtaining an emulsified composition by charging is preferred. These methods can be combined with mechanical emulsification using a propeller mixer, paddle mixer, disper mixer, homogenizer, or the like to stir, mix, and emulsify.

The appearance of the emulsion composition obtained in the present invention may be transparent, slightly turbid or cloudy, and the physical properties may be liquid, gel, cream or solid.

  The emulsion composition obtained in the present invention may be any of the W / O type, O / W type, and O / D type emulsion compositions, but the emulsion composition prepared by the above method is preferably O / D type emulsion is shown.

The emulsion composition obtained as described above can obtain an emulsion composition different from the above by diluting it with water. An emulsion composition that is an O / W emulsion can be obtained by diluting the emulsion composition that is an O / D emulsion with water. The dilution solvent is not particularly limited to water, and a water-soluble component generally used in cosmetics may be mixed. That is, there is no problem even if one or two or more mixed water-soluble components are used as the dilution solvent.

The appearance of the emulsion composition obtained by dilution may be slightly turbid or cloudy, and the physical properties may be liquid, gel, cream, or solid.

From the viewpoint of stability and usability of the emulsion composition after dilution, it is desirable that the appearance of the emulsion composition before dilution is a transparent gel, and by diluting the emulsion composition in this state, a fine emulsion It is expected to obtain a milky white emulsion composition consisting of

The emulsified composition obtained in the present invention can be applied to general cosmetics. Specifically, hair cosmetics such as shampoos, conditioners and treatments, face wash, cleansing cosmetics, sunscreen cosmetics, packs, massage cosmetics, skin lotions such as lotions, emulsions and creams, eyeliner, mascara Makeup cosmetics such as lipsticks and foundations, and bath cosmetics.

  EXAMPLES The present invention will be specifically described below with reference to examples, but the present invention is not limited to the examples.

(Production example)
Production Example 1 Production of MEL-A Pseudozyma antarctica (NBRC 10736) was cultured with aeration and stirring in a YM medium at a culture temperature of 26 ° C. for 48 hours using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The obtained seed culture solution was cultured with aeration and agitation in a YM medium (containing 5% olive oil) at a culture temperature of 26 ° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture and stirred for distribution. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer, and the mixture was allowed to stand for 30 minutes, and then concentrated by heating to obtain crude MEL-A. The obtained crude MEL-A was purified using a silica gel column with chloroform: acetone = 1: 0, chloroform: acetone = 9: 1, chloroform: acetone 1: 1, chloroform: acetone = 3: 7, chloroform: acetone = Elute with 0: 1. The MEL-A fraction was collected and concentrated to obtain purified MEL-A.

Production Example 2 Production of MEL-B Pseudozyma tsukubaensis (NBRC 1940) was cultured with aeration and agitation in a YM medium at a culture temperature of 26 ° C. for 48 hours using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The obtained seed culture solution was cultured with aeration and agitation in a YM medium (containing 5% olive oil) at a culture temperature of 26 ° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture and stirred for distribution. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer, and the mixture was allowed to stand for 30 minutes, and then concentrated by heating to obtain crude MEL-B. The obtained crude MEL-B was purified using a silica gel column with chloroform: acetone = 1: 0, chloroform: acetone = 9: 1, chloroform: acetone 1: 1, chloroform: acetone = 3: 7, chloroform: acetone = Elute with 0: 1. The MEL-B fraction was collected and concentrated to obtain purified MEL-B.

Production Example 3 Production of MEL-C Pseudozyma hubiensis KM-59 strain (FERM-20987) was aerated and agitated for 48 hours in a YM medium at a culture temperature of 26 ° C. using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The obtained seed culture solution was cultured with aeration and agitation in a YM medium (containing 5% olive oil) at a culture temperature of 26 ° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture and stirred for distribution. An appropriate amount of anhydrous sodium sulfate was added to the ethyl acetate layer, and the mixture was allowed to stand for 30 minutes, and then concentrated by heating to obtain crude MEL-C. The obtained crude MEL-C was purified using a silica gel column with chloroform: acetone = 1: 0, chloroform: acetone = 9: 1, chloroform: acetone 1: 1, chloroform: acetone = 3: 7, chloroform: acetone = Elute with 0: 1. The MEL-C fraction was collected and concentrated to obtain purified MEL-C.

(Example)
Using the sugar-type biosurfactant compounds obtained in the above Production Examples 1 to 3, emulsion compositions of Examples 1 to 18 and Comparative Examples 1 to 5 were prepared with the components and blending ratios shown in Tables 1 to 4.

(Conditions when preparing an emulsified composition)
MEL, purified water, and various anionic surfactants are mixed by heating at 50 to 60 ° C. with mixing ratios shown in Tables 1 to 4 below, and a polyhydric alcohol (glycerin) is added as necessary. The mixture is mixed uniformly again, and then mixed with various oil components (squalane, dimethylpolysiloxane, olive oil or tri (capryl / capric acid) glyceryl) gradually while stirring with a propeller mixer (1,000 rpm) to emulsify the composition. A product was prepared. MEL-A, MEL-B, and MEL-C were used as MEL. Moreover, as various anionic surfactants, cocoyl glutamate Na, laureth sulfate Na, cocoyl alanine TEA, lauroyl glutamate Na, stearoyl lactate Na, and a fatty acid potassium mixture were used.

(Evaluation method)
About the obtained emulsion composition, the property of the emulsion composition immediately after manufacture and the stability when stored in a thermostatic bath at 25 ° C. for 6 months are also shown in Tables 1 to 4. The properties were evaluated by visually observing the state of emulsification or separation.
◎: High transparency and maintain gel shape ○: Translucent to slightly turbid, but maintain gel shape ×: Separated, does not form gel

Moreover, the property evaluation also performed about the emulsion composition obtained by diluting this emulsion composition 10 times. When the emulsion composition was added to an aqueous solution containing 0.2% by mass (final concentration) of carboxyvinyl polymer so that the emulsified composition was 10% by mass, mixed by a conventional method, and then stored in a thermostatic bath at 25 ° C. for 6 months. The stability is also shown in Tables 1 to 4 as “Properties of the diluted solution (25 ° C., 6 months)”. The properties were evaluated by visually observing the state of emulsification or separation.
◎: Maintaining a good emulsified state of milky white ○: Maintaining an emulsified state of milky white to white ×: Separation

In Comparative Examples 1 to 5, when any component of MEL-B, anionic surfactant (cocoyl glutamate Na), oil component (squalane) or water was not blended, or 40 mass of oil component (squalane) When the content was relatively low, the mixture did not form a gel and separated immediately after preparation.

On the other hand, in Examples 1-16, the stable gel-like emulsion composition was able to be obtained by mix | blending MEL-B, an anionic surfactant, an oil-based component, and water as an essential component. Moreover, in Example 17, the stable gel-like emulsion composition was able to be obtained by mix | blending MEL-A, an anionic surfactant, an oil-based component, and water as an essential component. Furthermore, in Example 18, the stable gel-like emulsion composition was able to be obtained by mix | blending MEL-C, an anionic surfactant, an oil-based component, and water as an essential component.

In Examples, as an anionic surfactant, laureth sulfate Na classified as polyoxyethylene alkyl ether sulfate, cocoyl glutamate Na classified as N-acyl amino acid salt, cocoyl alanine TEA, lauroyl glutamate Na, acyl lactate The emulsion composition of the present invention was obtained using a mixture of stearoyl lactate Na and fatty acid potassium classified as a fatty acid soap, and the emulsified composition of the present invention was also related to these anionic surfactants and similar components. It is thought that it is possible to obtain things.

In Examples, the emulsion composition of the present invention was obtained using squalane, dimethylpolysiloxane, olive oil, and tri (capryl / capric acid) glyceryl as the oil component, but it contains components similar to these oil components. It is considered that the emulsion composition of the present invention can be obtained also for the oil component.

  According to the present invention, an emulsified composition using MEL, which is a sugar type biosurfactant compound, as a main emulsifier can be prepared. The emulsified composition of the present invention can emulsify various types of oily components. With this emulsified composition and its formulation, a very useful cosmetic preparation can be prepared, and it is expected to greatly contribute to the industry.

Claims (4)

(A) A sugar type biosurfactant, (B) an oil component, (C) water, (D) an anionic surfactant, and (E) a polyhydric alcohol, and (B) the amount of the oil component is 50. The blending ratio of (A) sugar type biosurfactant and (D) anionic surfactant is 10: 1 to 1: 2.5 (mass ratio),
(A) The sugar type biosurfactant is selected from the group consisting of mannosyl erythritol lipid (MEL), mannosyl mannitol lipid (MML), mannosyl sorbitol lipid (MSL), mannosyl arabitol lipid (MAraL) and mannosyl ribitol lipid (MRL). One or more compounds selected from the above, and
(D) The anionic surfactant is one or more surfactants selected from the group consisting of sodium cocoyl glutamate, sodium laureth sulfate, cocoyl alanine TEA, sodium lauroyl glutamate, sodium stearoyl lactate and fatty acid potassium mixture. ,
An emulsified composition which is an O / D type emulsion. The emulsification according to claim 1, wherein the sugar-type biosurfactant is mannosyl erythritol lipid A (MEL-A), mannosyl erythritol lipid B (MEL-B) and / or mannosyl erythritol lipid C (MEL-C). Composition. (A) Sugar type biosurfactant, (C) water and (D) anionic surfactant are mixed and dissolved, and further (E) polyhydric alcohol is dissolved, and then (B) 50.0 mass of oil component is stirred. The method for producing an emulsified composition , which is an O / D type emulsion, added so as to be at least%. The manufacturing method of the emulsion composition which is an O / W type emulsion including the process of further diluting the O / D type emulsion of Claim 3 with water.