JP2009079030A - Oily gel composition and method of preparation of emulsified composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an oily gel composition containing a glycolipid-type biosurfactant and a method of preparation of the emulsified composition. <P>SOLUTION: The oily gel composition contains a biosurfactant having a glycolipid structure as a surfactant, at least either of a polyhydric alcohol or water and an oily component. The method of preparation of the emulsified composition comprises mixing water with the oily gel composition. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an oily gel composition containing a biosurfactant having a glycolipid structure as a surfactant and a method for preparing an emulsified composition using the oily gel composition.

  Oily gel compositions are widely used as cosmetic additives. Methods for obtaining an oily gel composition include a method of blending silicic acid anhydride with a liquid oily component, a method of blending a metal soap, a method of blending a dextrin fatty acid ester, a polyhydric alcohol and a nonionic surfactant A method of blending is known. Furthermore, Patent Document 1 discloses a gel emulsion containing a glycolipid type nonionic surfactant such as sorbitan fatty acid ester and glycerin fatty acid ester.

  Cosmetics using anionic surfactant as a raw material are also widely used. For example, Patent Document 2 discloses an oily ointment base using an anionic surfactant. The oily ointment base described in Patent Document 2 is a stable oily ointment base comprising a hydrophilic polyglycerin higher fatty acid ester of HLB (Hydrophilic Lipophilic Balance) 9 or more, an anionic surfactant, water, and an oil phase component. And is excellent in the release of the formulated drug.

  On the other hand, biosurfactant is one of promising materials that can be used as a novel cosmetic additive, and its research is progressing. A biosurfactant is a functional substance produced by a microorganism, and is an amphiphilic substance having a hydrophobic part and a hydrophilic part. As biosurfactants, for example, surfactin having a lipopeptide structure and succinoyl trehalose lipid (STL) which is a glycolipid type are known.

As a cosmetic using a biosurfactant, for example, Patent Document 3 discloses an oil-based thickening gel-like composition containing surfactin as a surfactant.
JP 59-46123 A (published March 15, 1984) JP 61-257916 A (published November 15, 1986) JP 2003-176221 A (published on June 24, 2003)

  Among biosurfactants, glycolipid type biosurfactants are attracting attention as natural surfactants exhibiting excellent biodegradability. However, a glycolipid type biosurfactant capable of forming a gel composition and an emulsion composition is not yet known, and development of a gel composition using a glycolipid type biosurfactant is desired.

  The present invention has been made in view of the above problems, and an object thereof is to provide an oily gel-like composition containing a glycolipid type biosurfactant as a surfactant. Another object of the present invention is to provide a method for preparing an emulsified composition using the oily gel composition.

  As a result of intensive studies to solve the above-mentioned problems, the inventors of the present invention include a biosurfactant having a glycolipid structure as a surfactant, and includes an oily gel containing one of water and a polyhydric alcohol, and an oily component. It has been found that a composition can be prepared. Furthermore, it discovered that an emulsion composition was formed using the oil-based gel-like composition concerning this invention, and came to complete this invention.

  That is, the oily gel composition according to the present invention contains a biosurfactant having a glycolipid structure as a surfactant, and is characterized by containing at least one of water and a polyhydric alcohol, and an oily component.

  The oily gel composition according to the present invention preferably contains 0.01% by mass to 50% by mass of a biosurfactant having a glycolipid structure. The concentration of the biosurfactant is a concentration relative to the oily gel composition.

  Furthermore, the oily gel composition according to the present invention preferably contains 1% by mass to 89.9% by mass of water and polyhydric alcohol. The polyhydric alcohol preferably contains at least one of glycerin and ethylene glycol. In addition, the density | concentration of what combined said water and a polyhydric alcohol is a density | concentration with respect to an oil-based gel-like composition.

  Further, the oily gel composition according to the present invention preferably contains 10% by mass to 98.9% by mass of an oily component. The oil component preferably contains at least one of squalane, liquid paraffin, and silicone oil. In addition, the density | concentration of said oily component is a density | concentration with respect to an oily gel-like composition.

  Furthermore, in the oily gel composition according to the present invention, the biosurfactant having a glycolipid structure is preferably succinoyl trehalose lipid. Succinoyl trehalose lipid can be extracted in large quantities from a culture solution in which microorganisms are cultured.

  Furthermore, in the oily gel composition according to the present invention, the succinoyl trehalose lipid is preferably a salt with at least one of an alkali metal and an alkaline earth metal. Among these, a salt with sodium is preferable. By using a salt with alkali metal or alkaline earth metal as succinoyl trehalose lipid, succinoyl trehalose lipid can be dissolved well in an aqueous medium such as water and alcohol.

  The method for preparing an emulsified composition according to the present invention is characterized in that water is mixed with the oily gel composition according to the present invention. Moreover, in the preparation method of the emulsion composition concerning this invention, it is preferable to mix 50 mass% or more and 100000 mass% or less of water with respect to the total mass of the said oil-based gel-like composition.

  ADVANTAGE OF THE INVENTION According to this invention, the novel oily gel-like composition which contains the biosurfactant which has a glycolipid structure as surfactant can be provided. Moreover, according to the preparation method of the emulsion composition concerning this invention, the state which the aqueous component and the oil-based component were uniformly disperse | distributed can be maintained over a long period of time, and a highly stable emulsion composition can be obtained.

[Oil-based gel composition]
The oily gel-like composition of the present invention contains a biosurfactant having a glycolipid structure, at least one of water and a polyhydric alcohol, and an oily component. Hereinafter, an oily gel-like composition using succinoyl trehalose lipid as a biosurfactant having a glycolipid structure will be described with respect to one embodiment of the present invention.

[Succinoyl trehalose lipid]
First, succinoyl trehalose lipid used as a surfactant in the present invention and a method for producing the same will be described.

  The succinoyl trehalose lipid (hereinafter referred to as STL) used in the oily gel composition of the present invention is obtained by culturing a microorganism in a medium containing a carbon source.

  The succinoyl trehalose lipid obtained by culturing microorganisms in a medium containing a carbon source is a glycolipid in which the sugar moiety is trehalose and succinic acid and fatty acid are each linked by 1 to 2 moles per mole of trehalose. Different fatty acids are bound to the fatty acid portion of the glycolipid by changing the carbon source as a culture substrate.

  In the present specification, “microorganism” is intended to be a microorganism capable of producing STL. Such a microorganism is not particularly limited, but is preferably a microorganism belonging to the genus Rhodococcus, and is preferably Rhodococcus erythropolis SD-74 strain, Rhodococcus sp. TB-42 strain, or Rhodococcus baikonurensis NBRC 100611 strain. preferable.

  In the present specification, the “carbon source” is intended to be a carbon compound that is absorbed and utilized by the microorganism during culture. Such a carbon source is preferably a natural fat, hydrocarbon, fatty acid, fatty acid ester, or higher alcohol. Here, the carbon compound is intended to be a compound of carbon and hydrogen, nitrogen or the like. The carbon source used in the method for producing succinoyl trehalose lipid may be natural fats and oils, and may be animal fats or vegetable oils, but is preferably vegetable fats and oils because it is easier to obtain. The vegetable oil used in the above production method is preferably palm oil, coconut oil, soybean oil, olive oil, safflower oil, rapeseed oil, corn oil, cottonseed oil, tall oil, and the like, but is not limited thereto.

  Moreover, as hydrocarbon used as a carbon source, normal alkanes, such as n-decane, n-undecane, n-tridecane, n-tetradecane, n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane, n-nonadecane, etc. 1-decene, 1-undecene, 1-dodecene, 1-tridecene, 1-tetradecene, 1-pentadecene, 1-hexadecene, 1-heptadecene, normal alkene such as 1-octadecene, and the like are preferably used. Is possible.

  Fatty acids used as carbon sources include decanoic acid, undecanoic acid, dodecanoic acid (lauric acid), tridecanoic acid, tetradecanoic acid (myristic acid), pentadecanoic acid, hexadecanoic acid (palmitic acid), heptadecanoic acid, octadecanoic acid (stearic acid) , And oleic acid can be preferably used. Further, higher alcohols such as fatty acid esters, undecyl alcohol, and dodecyl alcohol (lauryl alcohol) may be used as the carbon source.

  The STL composition comprises a culture step of culturing a microorganism in a medium containing a carbon source, a precipitation step of precipitating a product obtained by the culture step, and an STL composition from the precipitate obtained by the precipitation step. It can be manufactured by a manufacturing method including an extraction step of extracting and a fat-soluble substance removing step of removing the fat-soluble substance from the extract obtained by the extraction step. Note that the STL composition is a composition containing STL as one component. In this embodiment, STL should just be contained 50 mass% or more in an STL composition, it is preferable that 80 mass% or more is contained, and it is more preferable that 90 mass% or more is contained. Hereinafter, a method for manufacturing the STL will be described in detail.

  The culture step of culturing the microorganism in a medium containing a carbon source is performed according to a conventional method, and a nutrient such as a nitrogen source and an inorganic salt may be added to the medium to which the carbon source is added, if necessary. As the carbon source added to the medium, each of the carbon sources described above is preferably used, and the concentration of the carbon source in the medium is preferably 5 to 20%, more preferably 10%. The nitrogen source added to the medium is a nitrogen-containing organic or inorganic substance commonly used in culturing microorganisms, such as sodium nitrate, potassium nitrate, potassium hydrogen phosphate, and potassium dihydrogen phosphate. It is. In addition to the above, if necessary for the growth of microorganisms, nutrients such as yeast extract and peptone may be added to the medium.

  The culture is carried out under aerobic conditions with shaking and stirring, and the culture temperature is preferably 20 to 35 ° C, more preferably 30 ° C. The culture pH is preferably 5.5 to 9.5. Moreover, it is preferable to culture until an STL mixture having a concentration of 15 to 50 g / l is generated during the culture period. In the examples described later, the STL concentration reaches 15 g / l after 5 days of culture. The culture is preferably performed for 5 to 12 days.

  In the step of culturing a microorganism in a medium containing a carbon source, seed culture may be performed before the microorganism is main-cultured. By seed-culturing microorganisms, it is possible to adjust microorganisms to optimum conditions, and as a result, it is possible to efficiently produce STL.

  Next, in the precipitation step, the product (STL) produced by the microorganism in the culture step is precipitated. That is, precipitation is performed on a medium or culture solution containing STL produced by microorganisms in the medium. At this time, the culture medium or the culture solution to be deposited may be obtained by centrifuging the culture solution in which the microorganism is cultured and removing the cells and the remaining substrate from the culture solution. Here, “precipitating” is intended to take out a substance dissolved in a medium as a solid. That is, in the precipitation step, the glycolipid produced in the medium by the microorganism in the culture step can be taken out as a solid from the medium.

  The precipitation step is not particularly limited as long as it is a method capable of separating STL as a solid from the culture solution, and a conventional method can be used. For example, STL, which is an acidic glycolipid, can be precipitated by acidifying the medium in which the microorganisms are cultured in the culturing step and precipitating an acidic substance in the medium (acid precipitation). Specifically, precipitation is performed by lowering the pH in the culture solution. In order to lower the pH of the culture solution, an acidic substance such as HCl may be added. Thereafter, for example, a centrifugal treatment is performed and the precipitate is taken out. Hereinafter, the product obtained through the precipitation step is referred to as “deposition product”.

  Next, in the extraction step, the STL composition is extracted from the precipitated product. In the extraction step, the STL contained in the precipitation product is dissolved in the solvent layer by adding a solvent that is incompatible with water and soluble in glycolipid to the precipitation product. Subsequently, the STL composition from which the water-soluble substance has been removed can be obtained by separating the solvent layer in which the STL is dissolved. The “water-soluble substance” is a substance that is soluble in water, and can be said to be a water-soluble impurity such as a salt contained in the solid precipitate precipitated from the medium together with water. . Here, the “salt” is a compound in which a hydrogen atom of an acid is replaced with a metal or other metallic group.

  The above-described precipitation product precipitated from the culture medium in which the microorganism is cultured contains water and contains a large amount of water-soluble impurities. Such a precipitation product further contains fat-soluble impurities and is in the form of a solid, so even if it is washed with water, it is difficult to sufficiently remove the water-soluble substance contained in the precipitate. However, an STL composition from which water-soluble substances have been removed can be obtained by an extraction process using a solvent that is incompatible with water and in which the glycolipid is soluble.

  In the extraction process, it is not necessary to completely remove the water-soluble substance from the precipitate, which is an object to be extracted by adding a solvent, and the amount of the water-soluble substance in the precipitate is reduced before and after the extraction process. That's fine. Thereby, it is possible to obtain an STL composition having a reduced impurity content.

  Here, as the solvent to be added to the precipitate, a solvent that is incompatible with another solvent (for example, water) that can dissolve the water-soluble substance and is soluble in glycolipid can be used. Examples of such solvents include ester solvents, alcohol solvents, and hydrocarbon solvents, and specific examples include ethyl acetate, 1-butanol, and xylene. The amount of the solvent added to the precipitate is preferably 0.1 to 10 times the weight of the precipitate, and more preferably the same as the amount of the precipitate.

  The extraction process will be described with reference to the case where ethyl acetate is used as a solvent. First, a culture product is precipitated from the culture solution, ethyl acetate is added to the deposited precipitate, and the mixture is sufficiently stirred and separated into two layers, an ethyl acetate layer and an aqueous layer. Next, the ethyl acetate layer formed in the upper layer is separated by a separatory funnel or the like. Since the water-soluble substance is not dissolved in the ethyl acetate layer and the STL is dissolved, it is possible to remove the water-soluble substance from the glycolipid by separating the ethyl acetate layer. Next, by removing ethyl acetate from the ethyl acetate layer using, for example, an evaporator or the like, a solid STL composition from which the water-soluble substance has been removed can be obtained.

  In the above description, the case where the precipitation process and the extraction process are sequentially performed has been described, but the present invention is not necessarily limited thereto. For example, an STL composition from which a water-soluble substance has been removed is obtained by adding a solvent to a medium or culture solution in which the product is dissolved without precipitating the culture product from the medium or culture medium and separating the solvent layer. It is also possible. At this time, what remove | excluded the microbial cell and the residual substrate from the culture solution which culture | cultivated microorganisms by centrifugation etc. is good also as a target which removes a water-soluble substance using a solvent. That is, in the extraction step, a substance that is a target for removing a water-soluble substance using a solvent is a medium or culture solution containing STL produced by a microorganism in culture in the medium, or a solid state separated from a reaction system. STL mixtures of

  The product obtained through this extraction step is referred to as “extraction product”.

  Next, in the fat-soluble substance removing step, the fat-soluble substance is removed from the extraction product obtained in the extraction step. Here, the “fat-soluble substance” is intended to be a substance soluble in fat.

  The method for removing the fat-soluble substance from the solid STL composition from which the water-soluble substance has been removed is not particularly limited as long as it is a method capable of separating the STL and the fat-soluble substance, and a conventional method is used. Can be used. For example, the solvent is first distilled off from the extracted product, and then a solvent capable of separating the glycolipid and the fat-soluble substance is added to the solid obtained by distilling off the solvent, and the solvent layer is added. The glycolipid from which the fat-soluble substance has been removed can be obtained by removing. Thereby, fat-soluble impurities can be efficiently removed from the glycolipid.

  Here, the solvent capable of separating the glycolipid and the fat-soluble substance is a solvent in which the glycolipid is hardly soluble or insoluble and the fat-soluble substance is soluble. For example, when hexane is used as such a solvent, a water-soluble substance is removed from the product precipitated from the culture solution using the solvent, and then the solid obtained by distilling off the solvent is converted into hexane. Suspend and filter or centrifuge to remove hexane. Thereby, fat-soluble impurities can be efficiently removed from the STL composition, and STL used as a surfactant in the present invention can be obtained.

  STL obtained by the above-described production method is a white solid. Specifically, it is preferable that the absorbance of the solution when dissolved in methanol by 5 mass% is 0.05 or less over a wavelength range of 400 nm to 700 nm. Further, this STL is an STL that can be dissolved at least 1% by mass in water, and more particularly 5% by mass in water.

  Further, an STL salt can be obtained from the STL obtained by the above-described method by the following method. It mixes with water so that it may become 1 mass% aqueous solution, While stirring using a stirrer, arbitrary alkali is added and aqueous solution is neutralized and STL is dissolved. By lyophilizing the aqueous solution having a pH of 7, an STL salt can be obtained.

  The STL used in the oily gel composition according to the present invention is preferably used as a salt of an alkali metal such as sodium, potassium and lithium or an alkaline earth metal such as calcium, magnesium and beryllium. When STL is used as a salt with an alkali metal or a salt with an alkaline earth metal, STL can be well dissolved in a solvent such as water or a polyhydric alcohol.

  Among these, sodium and potassium are preferable, and sodium is particularly preferable. The sodium salt of STL is highly water-soluble and has the advantage that the solubility in a solvent is further improved.

  The STL content in the oily gel composition of the present invention is preferably 0.01% by mass to 50% by mass, and preferably 0.1% by mass to 20% by mass of the total amount of the oily gelled composition. More preferably, it is more preferably 0.5% by mass to 10% by mass. If the amount of STL is less than 0.01% by mass, the gel does not sufficiently gel, and if it exceeds 50% by mass, the viscosity becomes too high, which is not preferable. The total amount of the oily gel composition refers to the total amount of STL, water, alcohol, and oily components.

[Water, polyhydric alcohol]
The oily gel composition according to the present invention contains at least one of water and a polyhydric alcohol.

  The polyhydric alcohol used in the oily gel-like composition of the present invention is usually used in cosmetics, and any oily gel-like composition can be used without particular limitation as long as it can be prepared.

  Examples of such polyhydric alcohols include propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, ethylene glycol, diethylene glycol, polyethylene glycol, 1,3-propanediol, and 1,4-butanediol. 1,3-butanediol, sorbitol, maltitol, erythritol, pentaerythritol, glucose, saccharose, maltose, xylose, trehalose can be used.

  These may be used alone or in combination of two or more.

  Of these, glycerin, sorbitol, and propylene glycol are preferred, and glycerin is particularly preferred. Glycerin has the advantage of being suitable as a cosmetic material because glycerin itself has moisture retention and is highly safe.

  When water is contained in the oily gel composition of the present invention, the viscosity of the oily gel composition is lowered. Therefore, the viscosity of the oily gel composition can be adjusted by adjusting the content of water contained in the oily gel composition. Therefore, it is preferable to change the water content in accordance with the purpose of use of the oily gel composition.

  The total content of water and polyhydric alcohol in the oily gel composition of the present invention is preferably 1% by mass to 89.9% by mass of the total amount of the oily gel composition, and 5% by mass to 80% by mass. % Is more preferable, and 10 mass% to 70 mass% is further more preferable. When the total content of water and polyhydric alcohol is 1% by mass to 89.9% by mass, there is an advantage that an oily gel composition can be formed stably.

(Oil component)
The oily gel composition according to the present invention contains an oily component.

  The oil component is preferably liquid or pasty at 1 atm. 25 ° C., and particularly preferably liquid oil.

  Examples of such liquid oils include hydrocarbons, higher alcohol esters, higher fatty acid esters, triglycerides, silicone oils, higher alcohols, higher fatty acids, animal and vegetable oils, cholesterol fatty acid esters, and sterols. Sterol esters, polyphenols, and preferred are mineral oil, liquid paraffin, squalane, isopropyl palmitate, isopropyl myristate, isooctyl myristate, isotridecyl myristate, octadecyl myristate, octyldodecyl myristate, Stearyl cholesteryl ester, 2-ethylhexanoic acid triglyceride, cetyl 2-ethylhexanoate, sunflower oil, olive oil, jojoba oil, camellia oil, grape seed oil, avocado oil Dimethyl silicone oils such as macadamia nut oil, almond oil, rice germ oil, clove oil, orange oil, spruce oil, octamethyltrisiloxane, decamethyltetrasiloxane, methylpolysiloxane, highly polymerized methylpolysiloxane; octamethylcyclotetrasiloxane , Cyclic silicone oils such as decamethylcyclopentasiloxane, dodecamethylcyclohexacyclosan, methylcyclopolysiloxane, and methylpolycyclosiloxane; polyether-modified silicone oils, methylphenyl silicone oils such as methylphenylpolysiloxane, and the like.

  These may be used alone or in combination of two or more.

  The content of the oil component in the oily gel composition of the present invention is preferably 10 to 98.9 mass%, more preferably 20 to 95 mass%, and more preferably 30 mass% of the total amount of the oily gel composition. It is more preferable that it is% -90 mass%. When the oil component is in the above range, there is an advantage that an oily gel composition can be stably formed.

  In addition, the external appearance of the oil-based gel-like composition of this invention can be made transparent by adjusting the composition.

[Other additives]
The oil-based gel composition of the present invention may contain an ultraviolet absorber as long as the effects of the present invention are not impaired. The ultraviolet absorber is a substance that is usually used in sunscreen cosmetics and the like, and can reduce ultraviolet A wave, ultraviolet B wave, or both, and reduce the harmful effects of ultraviolet rays on the skin.

  Such ultraviolet absorbers include p-aminobenzoic acid, glyceryl-p-aminobenzoic acid, amyl-p-N, N-dimethylaminobenzoic acid, 2-ethylhexyl-pN, N-dimethylaminobenzoic acid. P-aminobenzoic acid derivatives such as methyl 2,4-diisopropylcinnamate, ethyl 2,4-diisopropylcinnamate, potassium p-methoxycinnamate, sodium p-methoxycinnamate, p-methoxycinnamate Cinnamic acid derivatives such as isopropyl acid, 2-ethylhexyl p-methoxycinnamate, 2-ethoxyethyl p-methoxycinnamate, ethyl p-ethoxycinnamate; 2,4-dihydroxybenzophenone, 2,2 ′, 4 , 4'-tetrahydroxybenzophenone, 2-hydroxy-4-methoxy-5-sulfobenzophenone sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone, 2,2′-dihydroxy-4,4′-dimethoxybenzophenone, 2,2′-dihydroxy-4,4′- Benzophenone derivatives such as dimethoxy-5-sulfobenzophenone sodium; salicylic acid derivatives such as 2-ethylhexyl salicylate, phenyl salicylate, salicylic acid-3,3,5-trimethylcyclohexyl; 2- (2′-hydroxy-5′-methoxyphenyl) Examples include benzotriazole and 4-tert-butyl-4′-methoxybenzoylmethane.

  Among these, those that are solid at room temperature can be used by dissolving or dispersing in liquid oil. The liquid or paste-like material at normal temperature can be used as a liquid oil itself, or can be used by mixing with other liquid oils. Among those that can themselves be used as liquid oils, 2-ethylhexyl p-methoxycinnamate or 2-ethylhexyl p-methoxycinnamate in 4-tert-butyl-4′-methoxybenzoylmethane is preferred. is there.

  Moreover, an antioxidant and a fragrance | flavor can also be mix | blended with the oil-based gel-like composition of this invention in the range which does not impair the effect of this invention. Those which are solid at room temperature can be used by dissolving or dispersing in liquid oil. The liquid or paste-like material at normal temperature can be used as a liquid oil itself, or can be used by mixing with other liquid oils. Antioxidants that can be used include, for example, tocopherol, tocopherol acetate, and vitamins A (for example, retinoic acid, retinoic acid ester, retinol, retinoid, etc.).

  Applications of the oily gel composition of the present invention preferably include cosmetics, for example, basic cosmetics such as creams, lotions, cleansing gels, cleansing creams, and makeup makeups such as foundations, eye shadows, lip colors, lip glosses, etc. Cosmetics for hair such as hair creams, styling gels and hair waxes; cleaning agents for shampoos, rinses, hand soaps, body soaps, facial cleansing foams, etc.

  When the oily gel composition is used for cosmetics, any component usually used in cosmetics can be blended within a range that does not impair the effects of the present invention.

  Examples of such components include hydrocarbons such as petrolatum and microcrystalline wax; esters such as octyldodecyl myristate and isopropyl myristate, glyceryl triisooctanoate and triglycerides such as olive oil; methylphenylpolysiloxane, methyl Silicone oils such as polysiloxane; higher alcohols such as cetanol and behenyl alcohol; fatty acids such as stearic acid and oleic acid; polyhydric alcohols such as glycerin, 1,3-butanediol, and propylene glycol; ethanol, isopropyl alcohol, etc. Lower alcohols: nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, thickeners, UV absorbers, antioxidants, emollients, emulsifiers, Solubilizing agents, anti-inflammatory agents, humectants, preservatives, pH adjusting agents, dyes, perfumes, powders such, and water.

  Preferred optional components to be added when the oily gel-like composition is used for cosmetics are nonionic surfactants, higher fatty acids, and higher alcohols. Of these, stearic acid and behenyl alcohol are preferred. These preferable contents are 0.01 mass%-10 mass% with respect to cosmetics whole quantity, More preferably, they are 0.1 mass%-5 mass%. The cosmetics thus obtained have little skin irritation and are extremely excellent as cleansing agents, moisturizers, creams and lotions.

[Preparation method of oily gel composition]
The oily gel composition of the present invention can be prepared, for example, by the following method. First, it can be prepared by dissolving the sodium salt of STL in polyhydric alcohol or water, and adding oily components little by little while stirring. When polyhydric alcohol and water are used in combination, water may be added after the oil component is added.

  The oily component may be added in a predetermined amount (split addition) or continuously (continuous addition). In the case of divided addition, an amount of 30% by mass or less, preferably 20% by mass or less, more preferably 10% by mass or less is added at a time with respect to the total amount of polyhydric alcohol and water already added. Stir to homogenize. Add the required amount by repeating it.

  In the case of continuous addition, the amount added per unit time is not particularly limited as long as the added oily component is not separated but is mixed uniformly.

  In addition, when other components are added, they may be added before the oily component is added, dissolved or dispersed in the oily component, added after the total amount of the oily component is added, Any method may be used even if it is added during the addition of the components. The polyhydric alcohol and water may be added all at first, or a part of the added amount may be used first and the rest added later.

  According to the present invention, a novel oily gel composition containing succinoyl trehalose lipid, which is a glycolipid type biosurfactant, as a surfactant can be provided. In the oily gel composition of the present invention, the viscosity of the oily gel composition can be adjusted by adjusting the content of water. Therefore, application to various uses is possible. The oily gel composition of the present invention is excellent in hard water resistance. That is, for example, when the oily gel composition of the present invention is used as a cosmetic and the cosmetic is removed from the skin, the oily component is not separated even when using water with high hardness, and the occurrence of precipitates is prevented. Can do. Specifically, an oily gel composition in which oily components cannot be separated even when water having a hardness of 50 ppm or more is used is provided.

[Emulsion composition and preparation method of emulsion composition]
The method for preparing an emulsified composition according to the present invention includes a step of mixing water with the oily gel composition. As the oily gel composition, it is preferable to use the oily gel composition described above. Thereby, the liquid emulsion composition containing the biosurfactant mentioned above as a surfactant, an aqueous component, and an oily component can be obtained. In addition, in this specification, the composition obtained by emulsification is intended with an emulsion composition, and may be called an emulsion.

  Moreover, the emulsion composition obtained by the preparation method concerning this invention contains the biosurfactant which forms the liquid crystal, an oil-based component, and an aqueous component. Specifically, an oily component and an aqueous component are dispersed in the liquid crystal structure of the biosurfactant. Alternatively, it can be said that the oily component particles and the aqueous component particles are trapped in the liquid crystal structure of the biosurfactant. This is because the preparation method according to the present invention uses an oily gel-like composition in which a biosurfactant forms a liquid crystal.

  Therefore, according to the preparation method concerning this invention, isolation | separation with an aqueous component and an oil-based component is suppressed, and the emulsion composition stable over the long term can be provided.

  In addition, according to the preparation method according to the present invention, a method of preparing without going through the state of the oily gel, that is, a method of mechanically stirring and preparing the oily component and the aqueous solution of the biosurfactant using a homogenizer. In comparison, it is possible to provide a more stable emulsion composition in which separation of the aqueous component and the oily component is suppressed.

  The step of mixing water includes adding water to the oily gel composition and stirring. The mixing amount of water is preferably 50% by mass to 100000% by mass, more preferably 75% by mass to 80000% by mass, and more preferably 100% by mass to 60000% with respect to the total mass of the oily gel composition. % Is more preferable. When the mixing amount of water is less than 50% by mass, the viscosity is high and an emulsified composition cannot be obtained, that is, it remains in a gel state.

  The total amount of water to be mixed may be added at once (collective addition), divided into a plurality of parts (additional addition), or continuously (continuous addition). In the case of divided addition, the amount added once is 100% by mass or less, preferably 50% by mass or less, more preferably 10% by mass or less, based on the total mass of water to be added. Stir to homogenize. Add the required amount by repeating it. Next, stirring is repeated until the mixed solution becomes uniform. The emulsion composition concerning this embodiment can be obtained by the above method.

  According to the method for preparing an emulsified composition according to the present invention, a state in which an aqueous component and an oily component are uniformly dispersed can be maintained over a long period of time, and a highly stable emulsified composition can be obtained. Moreover, according to the preparation method of this invention, even if it is a case where water with large hardness is used as water added to an oily gel-like composition, a highly stable emulsion composition can be provided.

  The present invention is not limited to the above-described embodiments, and various modifications can be made within the scope shown in the claims, and embodiments obtained by appropriately combining technical means disclosed in different embodiments. Is also included in the technical scope of the present invention.

  EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited at all by these Examples.

[Production of STL]
Rhodococcus erythropolis SD-74 strain was seed-cultured under the following conditions. The Rhodococcus erythropolis SD-74 strain used in this example was isolated as a vegetable oil-fat assimilating bacterium and was registered with the Patent Organism Depositary at the National Institute of Advanced Industrial Science and Technology as “Accession Number: FERM AP-21299”. It has been deposited.

100 ml FPY medium in a 500 ml Sakaguchi flask (fructose 2%, polypeptone 0.5%, yeast extract 0.5%, NaNO ₃ 0.1%, KH ₂ PO ₄ 0.1%, MgSO ₄ -7H ₂ O 0. 02%) was inoculated with the bacterial colonies formed on the plate, and cultured with shaking at 30 ° C. for 38 hours.

Per modified MedD medium _{(1L, KH 2 PO 4 5.44g} , K 2 HPO 4 10.45g, KNO 3 3g, MgSO 4 -7H 2 O0.1g, K 2 SO 4 35g, yeast extract 3g, palm oil 100ml The total amount of the culture solution after seed culture was inoculated into 6000 ml, and the main culture was started under the following conditions. Using a 10 L jar culture tank, culture was performed at a culture temperature of 30 ° C. while stirring at 500 rpm. The pH of the medium was set to 7.0 and the pH of the medium was maintained by automatically adding 5N KOH.

  Sampling was performed about once every two days from the medium during the main culture, and the STL concentration in the culture solution was quantified as shown below.

  The sampled culture solution was centrifuged at 15000 rpm for 10 minutes, and the aqueous layer was extracted with care so that the upper oil component was not mixed. After the extracted liquid was appropriately diluted, the trehalose concentration was quantified by the following anthrone sulfate method. First, 5 ml of anthrone reagent (adjusted at the time of measurement by dissolving anthrone in 75% sulfuric acid at a concentration of 0.2%) was added to 1 ml of the diluted sample and stirred. The stirred solution was reacted in boiling water for 10 minutes, quenched with ice for 5 minutes, and allowed to stand at room temperature for 20 minutes.

  The absorbance at a wavelength of 620 nm was measured for the obtained reaction solution. A standard obtained by diluting 4 mM trehalose 20 times was used. Since STL contains one molecule of trehalose, the STL concentration was quantified as the trehalose concentration. The mass concentration of STL was calculated by setting the molecular weight of STL to 840. After 120 hours from the start of cultivation, 600 ml of palm oil was added, and after 200 hours, 300 ml of palm oil was further added. After 285 hours of culture, the STL concentration of the culture solution reached 37 g / L.

  860 ml of the culture solution after 285 hours of culture was centrifuged at 6000 rpm for 30 minutes to remove cells and residual substrate in the solution, and then 40 ml of 6N HCl was added to adjust the pH of the solution to 2.98. Thereby, a white gel-like precipitate was deposited in the solution. As a result of removing the liquid layer by centrifuging this solution at 6000 rpm for 30 minutes, a precipitate having a wet weight of 182 g was obtained.

  186 g of ethyl acetate was added to the resulting precipitate and stirred thoroughly. The solution separated into the aqueous layer and the ethyl acetate layer was separated using a separatory funnel, and the upper ethyl acetate layer was recovered. Ethyl acetate was removed from the collected ethyl acetate solution using an evaporator. After suspending the solid substance obtained by removing ethyl acetate with an equal amount of hexane, the process of centrifuging the suspension to remove hexane was repeated three times. The liquid obtained by removing hexane was dried with an evaporator to obtain 12.6 g of a white STL solid.

  STL obtained by the above production method was dissolved in methanol to prepare an STL solution having a concentration of 5% by mass. The absorption spectrum in the visible light region of the STL solution was measured using a spectrophotometer (UV-2550, Shimadzu Corporation). As a result, the STL solution did not exceed 0.05 over the wavelength range of 400 nm to 700 nm.

  The obtained STL was mixed with water so as to be a 1% by mass aqueous solution, and neutralized by adding sodium hydroxide while stirring with a stirrer to dissolve the STL. The aqueous solution finally adjusted to pH 7 was lyophilized to prepare a sodium salt of STL.

[Oil-based gel composition]
The oily gel-like compositions shown in Examples 1 to 6 were prepared using STL sodium salt purified by the above-described method (hereinafter referred to as STL sodium). All preparations were performed at room temperature. In the following examples, “%” means “% by mass”.

(Example 1: Glycerin-squalanger)
In Example 1, an oily gel composition in which the concentration of each component was 79% squalane, 20% glycerin, and 1% STL sodium was prepared. First, STL sodium was dissolved in glycerin, and squalane was added in portions of 10% by mass of the already added glycerin while stirring and stirred until uniform to prepare an oily gel composition.

  The obtained oily gel composition was translucent.

(Example 2: Glycerin-liquid paraffin gel)
In Example 2, an oily gel composition in which the concentration of each component was 79% liquid paraffin, 20% glycerin, and 1% STL sodium was prepared. First, STL sodium was dissolved in glycerin, and liquid paraffin was added in portions of 10% by mass of the already added glycerin while stirring, and stirred until uniform to prepare an oily gel composition.

  The obtained oily gel composition was transparent.

(Example 3: ethylene glycol-squalane gel)
In Example 3, an oily gel composition in which the concentration of each component was 67% squalane, 32% ethylene glycol, and 1% STL sodium was prepared. First, STL sodium was dissolved in ethylene glycol, and squalane was added in portions of 10% by mass of the already added ethylene glycol while stirring, and stirred until uniform to prepare an oily gel composition.

  The obtained oily gel composition was translucent.

(Example 4: Glycerin-silicone oil gel)
In Example 4, an oily gel-like composition was prepared in which the concentration of each component was 79% silicone oil (KF-968, Shin-Etsu Silicone), 20% glycerin, and 1% STL sodium. First, STL sodium was dissolved in glycerin, and 10% by mass of the already added glycerin was added in portions while stirring, and stirred until uniform to prepare an oily gel composition.

  The resulting oily gel composition was white.

(Example 5: Glycerin-water-squalanger)
In Example 5, an oily gel composition in which the concentration of each component was 77% squalane, 19% glycerin, 3% water, and 1% STL sodium was prepared. First, STL sodium is dissolved in glycerin, and squalane is added in portions of 10% by mass of the already added glycerin while stirring, stirring until uniform, and further adding water and stirring until uniform. An oily gel composition was prepared.

  The obtained oily gel composition was transparent.

(Example 6: water-squalanger)
In Example 6, an oily gel-like composition in which the concentration of each component was 75% squalane, 20% water, and 5% STL sodium was prepared. First, STL sodium was dissolved in water, and squalane was added in portions of 10% by mass of already added water while stirring, and stirred until uniform to prepare an oily gel composition.

  The obtained oily gel composition was translucent.

(Example 7: Glycerin-water-silicone oil gel)
In Example 7, an oily gel in which the concentration of each component is 65.3% silicone oil (KF-968, Shin-Etsu Silicone), 16.5% glycerin, 17.4% water, and 0.8% STL sodium. A composition was prepared. First, STL sodium is dissolved in glycerin, and 10% by mass of the already added glycerin is added in portions while stirring, stirring until uniform, and further adding water and stirring until uniform Then, an oily gel composition was prepared.

  The obtained oily gel composition was transparent.

[Viscosity of oily gel composition]
The viscosity of the oily gel compositions of Examples 1 and 4 was measured using RDAII (Rheometric). As a measurement condition, the shear rate is 30 (1 / s).

  Under the above conditions, the viscosity of the oily gel composition of Example 1 is 19.3 (Pa · s), and the viscosity of the oily gel composition of Example 4 is 66.0 (Pa · s). Met.

[Method for preparing emulsified composition]
Next, an emulsified composition was prepared using the oily gel composition in Example 4. Specifically, water equal in weight to the total weight of the oily gel composition according to Example 4 was added. The addition of water was performed by dividing the total amount of water to be added into 10% by mass while stirring the oily gel composition. Thus, the prepared emulsion composition (emulsion) was milky white and was a uniform emulsion. The uniformity of the emulsion was evaluated based on whether or not separation of the oily component and the aqueous component was observed when visually observed.

[Hard water resistance of oily gel composition and stability of emulsion composition]
Next, using the oily gel composition in Example 1 above, the hard water resistance of the oily gel composition and the stability of the emulsion composition were tested by the following methods. In this experiment, the stability of the emulsified composition refers to the ability to maintain a uniformly dispersed state after once forming an emulsion.

50 mg of the oily gel composition according to Example 1 was poured into a sample bottle, and 4950 mg of water having a hardness of 10 ppm, 50 ppm, 100 ppm, 250 ppm, or 500 ppm was added, respectively, and a test tube mixer (HM-10, Iuchi) ) For 1 minute. After mixing, the state of the liquid was visually observed, and the uniformity of the emulsion composition (emulsion) and the presence or absence of precipitates were evaluated. As water of each hardness, an aqueous solution prepared by dissolving CaCl ₂ -2H ₂ O in pure water and adjusting to 10 ppm, 50 ppm, 100 ppm, 250 ppm, and 500 ppm in terms of CaCO ₃ was used.

  In addition, an oily gel-like composition containing a surfactant surfactin sodium salt (hereinafter referred to as surfactin sodium) was prepared (Comparative Example 1), and the oil-based gel composition according to Comparative Example 1 was also resistant. Hard water test was performed. In the oily gel composition of Comparative Example 1, surfactin sodium was dissolved in glycerin so that the concentration of each component was 79% squalane, 20% glycerin, and 1% surfactin sodium, and squalane was already added. 10% by mass of the glycerin was added in portions while stirring, and stirred until uniform to prepare.

  Table 1 shows the results in Example 1 and Comparative Example 1.

  As shown in Table 1, by using the oily gel composition of Example 1, even in water having a hardness of 500 ppm, a uniform emulsion composition (emulsion) is obtained, the oil is not separated, and the precipitates are also observed. I couldn't. That is, according to the present Example, even if it was a case where hardness was high, the emulsion composition excellent in stability was able to be provided. On the other hand, when the oily gel-like composition in Comparative Example 1 was used, the oily component was separated in water having a hardness of 50 ppm, and a precipitate that appeared to be a calcium salt of surfactin appeared. The oily component and the precipitate were entangled to form a scum.

  Surfactin has low water resistance and is known to form insoluble salts when it contains divalent metal ions such as calcium ions and magnesium ions at a concentration of about 10 ppm. Therefore, as expected from the results shown in Table 1, cosmetics using an oil-based thickening gel-like composition containing surfactin have a problem that an insoluble salt precipitates when used with water having high hardness. Can occur.

  The hardness of tap water in Japan varies from region to region, but is between 0 ppm and 180 ppm, and on average is 50 ppm to 60 ppm. Furthermore, many European waters have high hardness, and there are regions of 300 ppm or more. Therefore, the above problem can occur in many areas.

  When the cleansing gel using the oily gel-like composition is washed away with water, when the oily gel-like composition of the present invention is used, it can be removed from the skin without being sticky just by washing with water. However, when the oily gel composition of Comparative Example 1 is used, when washing with water, the oily component is separated from the gel, which may make it difficult to remove from the skin.

  The present invention provides an oily gel-like composition suitable for cosmetics such as cleansing cosmetics, moisturizing cosmetics, and emulsifiers because it is white, translucent, or transparent and can contain a large amount of oily components. The present invention also provides an emulsified composition suitable for cosmetics such as creams and lotions.

Claims (13)

  An oily gel-like composition comprising a biosurfactant having a glycolipid structure as a surfactant and comprising at least one of water and a polyhydric alcohol, and an oily component.   The oily gel-like composition according to claim 1, comprising 0.01% by mass to 50% by mass of the biosurfactant having the glycolipid structure.   3. The oily gel composition according to claim 1, wherein the water and the polyhydric alcohol are contained in an amount of 1% by mass to 89.9% by mass.   The oily gel composition according to any one of claims 1 to 3, wherein the oily component is contained in an amount of 10% by mass to 98.9% by mass.   5. The oily gel composition according to any one of claims 1 to 4, wherein the polyhydric alcohol contains at least one of glycerin and ethylene glycol.   The oily gel composition according to any one of claims 1 to 5, wherein the oily component contains at least one of squalane, liquid paraffin, and silicone oil.   The oily gel-like composition according to any one of claims 1 to 6, wherein the biosurfactant having a glycolipid structure is succinoyl trehalose lipid.   The oily gel composition according to claim 7, wherein the succinoyl trehalose lipid is a salt with at least one of an alkali metal and an alkaline earth metal.   The oily gel composition according to claim 7 or 8, wherein the succinoyl trehalose lipid is a sodium salt.   The oily gel-like composition according to any one of claims 1 to 9, wherein the biosurfactant forms a liquid crystal.   A method for preparing an emulsified composition, comprising mixing the oily gel composition according to any one of claims 1 to 10 with water.   The method for preparing an emulsified composition according to claim 11, wherein 50 mass% or more and 100000 mass% or less of water is mixed with respect to the total mass of the oily gel composition.   An emulsified composition obtained by the preparation method according to claim 11 or 12.