JP6709778B2 - 無細胞ＤＮＡ（ｃｆＤＮＡ）の定量的遺伝子解析のための方法 - Google Patents

無細胞ＤＮＡ（ｃｆＤＮＡ）の定量的遺伝子解析のための方法 Download PDF

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JP6709778B2
JP6709778B2 JP2017510397A JP2017510397A JP6709778B2 JP 6709778 B2 JP6709778 B2 JP 6709778B2 JP 2017510397 A JP2017510397 A JP 2017510397A JP 2017510397 A JP2017510397 A JP 2017510397A JP 6709778 B2 JP6709778 B2 JP 6709778B2
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cfdna
dna
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JP2017525371A (ja
JP2017525371A5 (enExample
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クリストファー ケー． レイモンド，
クリストファー ケー． レイモンド，
リー ピー． リム，
リー ピー． リム，
クリストファー ディー． アーマー，
クリストファー ディー． アーマー，
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レゾリューション バイオサイエンス， インコーポレイテッド
レゾリューション バイオサイエンス， インコーポレイテッド
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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JP2017510397A 2014-08-22 2014-08-22 無細胞ＤＮＡ（ｃｆＤＮＡ）の定量的遺伝子解析のための方法 Active JP6709778B2 (ja)

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Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US10179937B2 (en) 2014-04-21 2019-01-15 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
WO2014071419A2 (en) 2012-11-05 2014-05-08 Foundation Medicine, Inc. Novel fusion molecules and uses thereof
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
TWI895765B (zh) 2014-07-18 2025-09-01 香港中文大學 Dna混合物中之組織甲基化模式分析
JP7441584B6 (ja) * 2014-08-22 2024-03-15 レゾリューション バイオサイエンス， インコーポレイテッド 無細胞ＤＮＡ（ｃｆＤＮＡ）の定量的遺伝子解析のための方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
AU2016353133B2 (en) * 2015-11-11 2022-12-08 Resolution Bioscience, Inc. High efficiency construction of dna libraries
WO2017161175A1 (en) * 2016-03-16 2017-09-21 Dana-Farber Cancer Institute, Inc. Methods for genome characterization
CN109477138A (zh) 2016-04-15 2019-03-15 纳特拉公司 肺癌检测方法
WO2017190067A1 (en) * 2016-04-28 2017-11-02 Impact Genomics, Inc. Methods of assessing and monitoring tumor load
EA201892491A1 (ru) * 2016-04-29 2019-06-28 Дзе Медикал Колледж Оф Висконсин, Инк. Пцр в реальном времени с мультиплексной оптимизированной амплификацией неправильно спаренных оснований (moma) для анализа злокачественных новообразований
CN106047998B (zh) * 2016-05-27 2019-11-12 深圳市海普洛斯生物科技有限公司 一种肺癌基因的检测方法及应用
EP3482202A4 (en) * 2016-07-11 2020-03-18 Arizona Board of Regents on behalf of Arizona State University USE OF SWEAT AS A BIOFLUID FOR THE ANALYSIS AND IDENTIFICATION OF DISEASES
AU2017315769B2 (en) * 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US20180142299A1 (en) * 2016-11-17 2018-05-24 SeraCare Life Sciences,Inc Methods for preparing dna reference materials and controls
KR20230062684A (ko) 2016-11-30 2023-05-09 더 차이니즈 유니버시티 오브 홍콩 소변 및 기타 샘플에서의 무세포 dna의 분석
WO2018237075A1 (en) 2017-06-20 2018-12-27 The Medical College Of Wisconsin, Inc. Assessing transplant complication risk with total cell-free dna
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
US12071661B2 (en) * 2018-02-12 2024-08-27 Roche Sequencing Solutions, Inc. Method of predicting response to therapy by assessing tumor genetic heterogeneity
US11767562B2 (en) 2018-03-08 2023-09-26 St. John's University Circulating serum cell-free DNA biomarkers and methods
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
CN108517567B (zh) * 2018-04-20 2020-08-11 江苏康为世纪生物科技有限公司 用于cfDNA建库的接头、引物组、试剂盒和建库方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN108676891B (zh) * 2018-07-12 2022-02-01 吉林大学 一种直肠腺癌易感性预测试剂盒及系统
US11926821B2 (en) * 2018-10-22 2024-03-12 The Chinese University Of Hong Kong Cell-free DNA quality
WO2020149301A1 (ja) * 2019-01-15 2020-07-23 一般社団法人生命科学教育研究所 Ｐｖａスポンジを用いるリキッドバイオプシーの調製方法
US11931674B2 (en) 2019-04-04 2024-03-19 Natera, Inc. Materials and methods for processing blood samples
CN112301103B (zh) * 2019-08-02 2023-04-11 北京贝瑞和康生物技术有限公司 一种非特异性扩增天然短片段核酸的方法和试剂盒
CN110819622A (zh) * 2019-10-09 2020-02-21 广州达正生物科技有限公司 一种快速提取石蜡组织rna的方法
CN111254194B (zh) * 2020-01-13 2021-09-07 东南大学 基于cfDNA的测序及数据分析的癌症相关生物标记及其在cfDNA样品分类中的应用
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US20230323455A1 (en) * 2020-07-24 2023-10-12 Cornell University Methods for assessing the severity and progression of sars-cov2 infections using cell-free dna
ES3045482T3 (en) * 2020-09-08 2025-11-28 Resolution Bioscience Inc Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis
EP4399330A4 (en) * 2021-09-10 2025-07-16 Found Medicine Inc GENE FUSIONS IN SARCOMA

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023769B2 (en) * 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
EP2426217A1 (en) * 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
US20130288915A1 (en) * 2012-04-27 2013-10-31 Htg Molecular Diagnostics, Inc. Compositions and methods for alk molecular testing
CA2886605A1 (en) * 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
WO2014071295A1 (en) * 2012-11-02 2014-05-08 Enzymatics Inc. Methods and kits for nucleic acid sample preparation for sequencing
CN111254500B (zh) * 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
EP2954054B1 (en) * 2013-02-08 2018-12-05 Qiagen GmbH Method for separating dna by size

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CN107002118A (zh) 2017-08-01
EP4410978A3 (en) 2024-10-30
SG11201701113WA (en) 2017-03-30
JP2017525371A (ja) 2017-09-07
EP3194612B1 (en) 2024-07-03
PL3194612T3 (pl) 2024-11-04
CN107002118B (zh) 2022-07-08
PT3194612T (pt) 2024-10-02
CN115029342B (zh) 2025-04-08
DK3194612T3 (da) 2024-10-14
CA2957657A1 (en) 2016-02-25
HUE068191T2 (hu) 2024-12-28
EP3194612A1 (en) 2017-07-26
EP4410978A2 (en) 2024-08-07
ES2984266T3 (es) 2024-10-29
WO2016028316A1 (en) 2016-02-25
CN115029342A (zh) 2022-09-09

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