JP6700015B2 - Antifungal composition - Google Patents

Description

  The present invention relates to antifungal compositions containing silver halide nanoparticles.

  In recent years, in each field, there is a growing tendency to emphasize hygiene, and various antibacterial products have been developed, but the fact is that the development of products against fungi such as mold has not progressed so much. The reason is that bacteria are prokaryotic organisms that have no nucleus, whereas fungi are eukaryotic organisms that have the same nucleus as humans, so there are very few drugs with low cytotoxicity. Since it has a strong cell wall, it is difficult for the drug to reach the inside of the cell. In addition to superficial fungi that can be removed relatively easily by wiping, fungi can also be found in deep areas that are difficult to remove, such as black mold that grows in the rubber packing in the bathroom and ringworm that lives in the subcutaneous tissues. Superficial fungi are known, and the latter has not been fully developed.

  Therefore, as a method for solving these problems, a poorly soluble orthophosphoric acid double salt of two or more metals selected from the group consisting of magnesium, calcium, strontium, aluminum, manganese, nickel, copper, and zinc is used. An antibacterial/antifungal spray agent carrying 0.5 to 5.0% by weight of silver and (patent document 1), a remedy for athlete's foot with silver or copper supported on an inorganic porous material, and the like have been proposed (patented). Reference 2).

JP, 7-309706, A JP-A-6-009412

In Patent Documents 1 and 2, silver, which is known as an antibacterial agent, is used, but both have a problem that the nozzle is clogged when used as a propellant due to the large particle size, and the usage form is It was limited.
Therefore, the present invention provides an antifungal composition that can be used in more forms.

  In order to enable use in more forms, it is conceivable that the component acting as an antifungal agent has a nano size, but such a size tends to make the dispersion unstable. As a result of earnest research, the present inventor has found a composition having excellent dispersion stability even when nano-sized fine particles are used as an antifungal component. Furthermore, the present inventor has also found that the composition has excellent antifungal action, and has excellent durability and immediate effect.

  That is, the first invention is to provide nanoparticles comprising silver halide having a particle size of 1 nm or more and 100 nm or less, a halide ion of the same element as halogen constituting the silver halide, and at least one water-soluble polymer. And an organic acid having a carboxyl group and/or a salt thereof, and the molar ratio of silver to halide ion in the silver halide is 1:1 to 1:1000. It is a thing.

  A second invention is characterized in that, in the above-mentioned first invention, the antifungal composition further comprises at least one selected from the group consisting of a surfactant, a humectant, a solvent, and an antioxidant. It is an antifungal composition.

  According to the present invention, it is possible to provide an antifungal composition that can be used in various forms.

1 is a transmission electron microscope image of the antifungal composition obtained in Example 1.

  Hereinafter, one embodiment of the antifungal composition of the present invention will be described in detail. The antifungal composition of the present embodiment comprises nanoparticles of silver halide, a halide ion, a water-soluble polymer, an organic acid having a carboxyl group (hereinafter, also simply referred to as an organic acid) and/or a salt thereof. Including and

The antifungal composition of the present embodiment can be produced, for example, by mixing a silver salt, a halide, a water-soluble polymer, an organic acid and/or a salt thereof in a solution. By mixing the silver salt and the halide, the silver salt and the halide react with each other to produce silver halide. The ratio of each component is not particularly limited, but the ratio of silver salt and halide (so that the molar ratio of silver and halide ion contained in the produced silver halide is 1:1 to 1:1000 ( It is preferable to set the ratio of silver ions forming the silver salt and halide ions forming the halide.
The production method is not limited to the method, and for example, a halide ion, a water-soluble polymer, an organic acid and/or a salt thereof may be added to a composition containing silver halide nanoparticles that has already been obtained. It may be prepared as follows.

  The bactericidal property of the silver halide contained in the antifungal composition of the present embodiment has been conventionally known and used as various bactericidal compositions. In the present embodiment, among them, nanoparticles having a very small particle diameter of 1 nm or more and 100 nm or less can be used. Further, from the viewpoint of efficiently exhibiting antifungal properties even in a small amount, it is preferable that the silver halide nanoparticles have a small particle size. Also, from the viewpoint of facilitating penetration into the rigid cell wall of fungi, it is preferable that the silver halide nanoparticles have a small particle diameter. On the other hand, if the grain size is less than 1 nm, the stability of the silver halide as a substance is reduced. Therefore, in order to obtain good antifungal properties and good stability, the particle size of the silver halide nanoparticles is more preferably a single nanosize of less than 10 nm and 1 nm or more. The particle size can be controlled, for example, by adjusting the ratio of silver ions and halide ions when reacting the silver salt and the halide. Further, silver halide has low crystallinity, and an amorphous crystal with low crystallinity is synthesized by synthesizing at low temperature, and nanoparticles having good crystallinity can be obtained by synthesizing at high temperature. In this embodiment, any crystal can be used, and there is no particular limitation.

As described above, the silver halide nanoparticles can be obtained, for example, by reacting a silver salt with a halide.
Examples of silver salts include silver nitrate, silver nitrite, silver chlorate, silver perchlorate, silver acetate and silver sulfate, and halides include sodium chloride, potassium chloride, potassium iodide, lithium chloride, copper chloride ( II), silver chloride, chlorine fluoride and the like can be mentioned, and the combination thereof can be freely selected according to the silver halide to be actually used.

  In the preparation exemplified above as to the antifungal composition of the present embodiment, it is desirable that the halide is in a stoichiometric excess with respect to the silver salt with respect to the ratio of the silver salt and the halide. Specifically, the silver in the silver halide present in the antifungal composition of the present embodiment and the halide ion (the halide ion present as an ion in the composition without reacting with the silver ion) It is preferable to set the molar ratio of the silver ions forming the silver salt and the halide ions forming the halide so that the molar ratio thereof is 1:1 to 1:1000. Particularly, in the present embodiment, it is preferable that the difference in the molar ratio between the silver ion and the halide ion is larger because silver halide nanoparticles having a smaller particle size can be obtained.

When the ratio of halide ions is less than 1:1, all the halide ions react with silver ions in the preparation of the composition, and the electric double layer described below is not formed around the silver halide nanoparticles. As a result, the zeta potential of the silver halide nanoparticles decreases, the particles aggregate and settle, or the particles are bound to each other to increase in particle size and settle, resulting in dispersion of the silver halide nanoparticles. Stability decreases. On the other hand, when the ratio of halide ions is more than 1:1000, the halide ions become excessive and form a complex with silver.
In addition, the halide ion in the present specification means a fluoride ion (F-), a chloride ion (Cl-), a bromide ion (Br-), an iodide ion (I-), an astatine ion (At- ) And the like.
Further, when the step of mixing a solution of a silver salt and a solution of a halide is included in the preparation of the composition, the shorter the contact time between the two solutions is, the smaller the grain size of the silver halide nanoparticles is, so that the step is concerned. Is preferably performed in the shortest possible time.

  Here, the electric double layer formed around the silver halide nanoparticles in the present embodiment will be described. The halide exists as a halide ion in the solution, and reacts with the silver ion formed by dissolving the silver salt in water to form silver halide nanoparticles. The produced silver halide nanoparticles adsorb excess halide ions present in the solution and are negatively charged, and the adsorbed silver halide ions adsorb counter ions of the excess halide ions. As a result, an electric double layer is formed around the silver halide nanoparticles. The silver halide nanoparticles around which the electric double layer is formed have high zeta potential, and thus are stabilized in dispersion.

  In this embodiment, the dispersion-stabilized silver halide nanoparticles are protected by a water-soluble polymer as a dispersant. Further, the organic acid and/or its salt suppresses the aggregation of the silver halide nanoparticles. Thereby, the dispersion stability of the silver halide nanoparticles can be remarkably enhanced, so that the aggregation of the silver halide nanoparticles can be suppressed for a long period of time even if the particle size is several nm.

  The water-soluble polymer is not particularly limited as long as it enhances the dispersibility of silver halide nanoparticles, and examples thereof include water-soluble polymers such as hydroxypropyl cellulose, polyvinylpyrrolidone and polyvinyl alcohol. These water-soluble polymers have various molecular weights, and any of them can be used. However, as the molecular weight becomes higher, silver halide nanoparticles having a smaller particle size can be obtained. Water-soluble polymers are preferably used.

  The organic acid having a carboxyl group (-COOH) and/or its salt is not particularly limited, but citric acid, lactic acid, malic acid, tartaric acid, succinic acid, ascorbic acid, glycolic acid, and oxy-acids of these salts and the like. Carboxylic acid is preferably used, and citric acid is more preferably used.

  When preparing a dispersion liquid containing dispersion-stable silver halide nanoparticles in this embodiment, it is important to prevent aggregation of the silver halide nanoparticles. Aggregation of silver halide nanoparticles is greatly affected by the zeta potential of the particles. Therefore, use organic acid and/or its salt to adjust the pH of the dispersion to the acidic side to increase the zeta potential of silver halide nanoparticles. Therefore, aggregation can be suppressed. In the present embodiment, the ratio of the water-soluble polymer and the organic acid and/or its salt is not particularly limited and can be set as appropriate, but the amount of the organic acid and/or its salt added is such that the pH of the aqueous solution is 2.0 to The amount is preferably adjusted to 6.0. In order to control the particle size of the silver halide nanoparticles, it is important to control the supply of silver ions, which is the main component of the silver halide nanoparticles, from the solution. In other words, how to inhibit the particle growth of nanoparticles due to silver ions remaining in the composition that do not contribute to the reaction during the synthesis of silver halide nanoparticles and silver ions that have been eluted from the nanoparticles due to aging. Is important. Since the organic acid and/or its salt easily forms a complex with silver ions and is stabilized, it is possible to prevent the above-mentioned silver ions from participating in grain growth. Of these, oxycarboxylic acids, especially citric acid, tend to form a complex with silver ions and stabilize. Therefore, using an oxycarboxylic acid (more preferably citric acid) as a pH adjuster is more preferable in synthesizing silver halide nanoparticles having an extremely small particle size of several nm and excellent dispersion stability. It is suitable.

  The antifungal composition of the present embodiment obtained by, for example, mixing the above components is not particularly limited in its form, and for example, in addition to solution form, sol, gel, etc., the composition exhibits fluidity. It is possible to have an aspect having.

  The antifungal composition of the present embodiment may further contain at least one selected from the group consisting of a surfactant, a solvent, and an antioxidant. These components are usually added to the composition after mixing with a silver salt, a halide, a water-soluble polymer, an organic acid and/or a salt thereof in a solution to form silver halide nanoparticles. In addition to the above, the present invention is not limited to this, and may be added before, for example, silver halide nanoparticles are formed.

  As the surfactant, an ionic surfactant, a nonionic surfactant, and the like can be appropriately used, but a nonionic surfactant is preferable from the viewpoint of irritation. Examples of the nonionic surfactant, polyoxyalkyl ether, glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, propylene glycol fatty acid ester, polyglycerin condensed ricinoleic acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, Examples thereof include polyoxyethylene fatty acid ester and polyoxyethylene sorbitan fatty acid ester. When a surfactant is blended, the antifungal composition of the present embodiment in the form of fine micelles is evenly distributed on the skin surface, and further, nanoparticles of silver halide as an active ingredient are easily penetrated into epidermal cells. Therefore, it becomes easy to contact deep surface superficial fungi such as Trichophyton which inhabit the keratin and subcutaneous tissues.

  When the antifungal composition of the present embodiment is used as a remedy for athlete's foot etc., a moisturizer etc. may be added. As humectants, various keratinic intercellular lipids such as phytosphingosine; hyaluronates such as sodium hyaluronate and its acid derivatives; polypropylene glycol, polyethylene glycol, ethylhexanediol, hexylene glycol, glycerin, propylene glycol , Various kinds of polyols such as sorbitol, hexanediol, and capryl glycol. Among these, ceramides and phytosphingosine are preferable because they have an excellent effect of keeping the skin moist and maintain the texture of the skin. Among them, ceramides are preferable because they are substances particularly required for damaged skin.

  As the solvent, in addition to water, alcohols such as ethanol and isopropanol for improving wettability; glycol ethers such as diethylene glycol monomethyl ether, diethylene glycol butyl ether, diethylene glycol monoethyl ether, and diethylene glycol dibutyl ether; polyethylene glycol- Polyethylene glycols such as 300 and polyethylene glycol-400; glycols such as propylene glycol and glycerin; pyrrolidones such as 2-pyrrolidone and N-methyl-2-pyrrolidone; glycerol formal; dimethyl sulfoxide; dibutyl sebecate; polysorbate 80 Organic solvents such as polyoxyethylene sorbitan ester; and mixtures thereof may be added.

  Further, in order to prevent the deterioration of the antifungal composition of this embodiment, an antioxidant may be preferably used. Specifically, sodium bisulfite, sodium sulfite, sodium metabisulfite, sodium thiosulfate, sodium formaldehyde sulfoxylate, l-ascorbic acid, erythorbic acid, acetylcysteine, cysteine, monothioglycerol, thioglycolic acid, thiolactic acid. , Thiourea, dithiothreitol, dithioerythreitol, glutathione, ascorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, nordihydroguaiaretic acid, propyl gallate, α-tocopherol, and mixtures thereof. It can be selected appropriately.

  The antifungal composition thus obtained can be used in various forms and methods.

  For example, as mentioned above, when using it for black mold in a bathroom, etc., put it in a spray etc. and spray it directly on the mold, or spray it in a place where black mold has not yet grown, to prevent the mold. Can be demonstrated. This is because the antifungal composition of the present embodiment is configured to include silver halide nanoparticles, and thus the sprayed silver halide remains in place and the effect is maintained for a long time.

  As another example, when used as an external medicine for athlete's foot etc., it may be put in a spray etc. and directly steamed on the affected area, or it may be attached by impregnating it with gauze or a non-woven fabric, petrolatum, paraffin, silicone, plastic base. , Ointment may be mixed with a known base such as vegetable oil, beeswax, and lanolin. Furthermore, since the silver halide nanoparticles according to the present embodiment have antibacterial properties as well as bactericidal properties, they can be sprayed onto shoes with poor air permeability, such as boots and leather shoes, and insoles. By impregnating and using it, not only can athlete's foot be prevented, but also the odor generated when bacteria decompose sweat or the like can be prevented. At this time, the deodorant property can be improved by using it together with a deodorant such as a masking agent.

  Examples of fungi in which the antifungal composition of the present embodiment exhibits effects include, for example, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans, Microsporum canis, Microsporum gypseum, Trichophyton verrucosum, and other tinea fungi, Aspergillus niger, Aspergillus ochraceus, and Aspergillus ochraceus Of Aspergillus niger, Eugenicillium, Hailer, Penicilliopsis, Talaromyces, Trichocoma that grows in foods, etc., Eurotium tonophilum, Penicillium citrinum, Penicillium pinophilum, Rhizopus oryzae Examples include Aureobasidium pullulans, Trichoderma virens, Chaetomium globosum, Myrothecium verrucaria, but are not limited thereto.

As described above, according to this embodiment, it is possible to provide an antifungal composition that can be used in more forms. For example, since nanoparticles of an inorganic compound called silver halide are used as one of the active ingredients, problems such as nozzle clogging are less likely to occur when a spray agent or the like is used. In addition, it can be sprayed over a wide range at the time of use, and can remain in place for a long time even after spraying to maintain the effect.
Further, the antifungal composition of the present embodiment contains a halide ion of the same type as the halogen element constituting the above silver halide. The halide ion is adsorbed on the silver halide nanoparticle, and an electric double layer is formed around the silver halide nanoparticle by the adsorption of the halide ion, and the electric double layer is formed on the periphery. Protect silver halide nanoparticles with a water-soluble polymer. Furthermore, the organic acid and/or salt thereof contained in the antifungal composition of the present embodiment suppresses aggregation of silver halide nanoparticles. Therefore, according to this embodiment, an antifungal composition having excellent dispersibility of the contained silver halide nanoparticles can be obtained. Since the silver halide grains are dispersed in the form of nanoparticles, an antifungal effect can be effectively obtained even in a small amount.
Further, in the antifungal composition of the present embodiment, by containing an oxycarboxylic acid such as citric acid as the organic acid and/or salt, it is possible to further suppress reaggregation of the silver halide nanoparticles. The performance can be maintained for a longer period of time.

  Next, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these examples.

(Example 1)
To 320 mL of 0.8 M potassium iodide solution (purity KI 99% (Wako Pure Chemical Industries)), 1% by volume of polyvinylpyrrolidone (Across organics, molecular weight 3500) and citric acid (purity 98% (Wako Pure Chemical Industries, Ltd.) 0.02% was added and stirred until completely dissolved. In another container, 50 mL of 0.5 M silver nitrate solution (purity 99.8% (manufactured by Wako Pure Chemical Industries)) was prepared in a shielded container. The antifungal composition of Example 1 was obtained by setting the molar ratio of silver ion: iodide ion to be 1:10 and stirring and mixing the above two solutions instantaneously. Here, the particle size of the silver iodide nanoparticles contained in the obtained antifungal composition is measured by a zeta potential/particle diameter measurement system (Otsuka Electronics, laser Doppler method (dynamic/electrophoretic light scattering method)) As a result, the average particle diameter at this time was 4.2 nm. The average particle size referred to here is the volume average particle size.
Further, the silver iodide nanoparticle composition was observed with a transmission electron microscope (JEM-2100 manufactured by JEOL Ltd.). The photograph is shown in FIG.

(Example 2)
1.0 wt% of the antifungal composition obtained in Example 1, 0.5 wt% of potassium iodide (purity KI 99% (manufactured by Wako Pure Chemical Industries)), citric acid monohydrate (purity 98% (manufactured by Wako Pure Chemical Industries, Ltd. )) 1.0 wt%, polyvinylpyrrolidone (Across organics, molecular weight 3500) 0.1 wt%, the rest is mixed as pure water, and the antifungal composition has a lower ratio of silver iodide to iodide ion as compared to Example 1. Was prepared as Example 2. The molar ratio of silver ion:iodide ion of this composition (derived from silver ion during solution mixing of Example 1: iodide ion during solution mixing of Example 1 and potassium iodide newly added in Example 2) The sum of iodide ions) was 1:56. The average particle size of the contained silver iodide nanoparticles was 3.8 nm.

(Example 3)
1.0 wt% of the antifungal composition obtained in Example 1, potassium iodide (purity KI 99% (manufactured by Wako Pure Chemical Industries)) 1 wt%, citric acid monohydrate (purity 98% (manufactured by Wako Pure Chemical Industries, Ltd.) ) 0.1 wt%, polyvinylpyrrolidone (manufactured by Across organics, molecular weight 3500) 0.1 wt%, and the rest was mixed as pure water to prepare an antifungal composition having a lower ratio of silver iodide to iodide ion as compared with Example 1. It was created as Example 3. Molar ratio of silver ion:iodide ion of this composition (derived from silver ion during solution mixing of Example 1: iodide ion during solution mixing of Example 1 and potassium iodide newly added in Example 3) The sum of iodide ions) was 1:100. The average particle size of the contained silver iodide nanoparticles was 2.5 nm.

(Example 4)
0.33 wt% of the antifungal composition obtained in Example 1, 3 wt% of potassium iodide (purity KI 99% (manufactured by Wako Pure Chemical Industries)), citric acid monohydrate (purity 98% (manufactured by Wako Pure Chemical Industries, Ltd.) ) 0.5 wt%, polyvinylpyrrolidone (manufactured by Across organics, molecular weight 3500) 0.1 wt%, and the rest was mixed as pure water to prepare an antifungal composition having a lower ratio of silver iodide to iodide ion as compared with Example 1. It was created as Example 4. Molar ratio of silver ion: iodide ion of this composition (derived from silver ion during solution mixing of Example 1: iodide ion during solution mixing of Example 1 and potassium iodide newly added in Example 4) Sum of iodide ions) was 1:812. The average particle size of the contained silver iodide nanoparticles was 1.3 nm.

(Comparative Example 1)
Potassium iodide (purity KI 99% (Wako Pure Chemical Industries)) 3 wt%, citric acid monohydrate (purity 98% (Wako Pure Chemical Industries)) 0.1 wt%, polyvinylpyrrolidone (Across organics, molecular weight 3500) 0.1 A solution containing no silver iodide was prepared with wt% and the rest being pure water.

(Comparative example 2)
0.5 wt% potassium iodide (purity KI 99% (Wako Pure Chemical Industries)), citric acid monohydrate (purity 98% (Wako Pure Chemical Industries)) 1.0 wt%, polyvinylpyrrolidone (Across organics, molecular weight 3500) A solution containing no silver iodide was prepared by using 0.1 wt% and pure water as the rest.

(Comparative example 3)
Potassium iodide (Purity KI 99% (Wako Pure Chemical Industries)) 1.0 wt%, citric acid monohydrate (Purity 98% (Wako Pure Chemical Industries)) 0.1 wt%, polyvinylpyrrolidone (Across organics, molecular weight 3500) A solution containing no silver iodide was prepared by using 0.1 wt% and pure water as the rest.

(Comparative example 4)
Potassium iodide (Purity KI 99% (Wako Pure Chemical Industries)) 3.0 wt%, citric acid monohydrate (Purity 98% (Wako Pure Chemical Industries)) 0.5 wt%, polyvinylpyrrolidone (Across organics, molecular weight 3500) A solution containing no silver iodide was prepared by using 0.1 wt% and pure water as the rest.

(Comparative Example 5)
0.1 wt% of the antifungal composition obtained in Example 1, 1.4 wt% of potassium iodide (purity KI 99% (manufactured by Wako Pure Chemical Industries)), citric acid monohydrate (purity 98% (manufactured by Wako Pure Chemical Industries, Ltd.) )) 0.1 wt% and the rest was pure water to prepare a composition having a low proportion of silver iodide. Molar ratio of silver ion:iodide ion of this composition (silver ion during solution mixing of Example 1: iodide ion during solution mixing of Example 1 and potassium iodide newly added in Comparative Example 5) The sum of iodide ions) was 1:1250. The presence of silver iodide nanoparticles could not be confirmed.

(Moldproof test method)
The fungus used was Trichophyton mentagrophytes, which was cultured in a PDA slant medium. 10 ml of sterilized water containing a wetting agent (0.005% sodium dioctyl sodium sulfosuccinate solution) was placed in a test tube in which Trichophyton was cultured, and spores were scraped off with a platinum loop. The obtained solution was filtered with sterile gauze and adjusted with sterilized water containing a wetting agent so that the number of spores was 10 ⁶ cells/ml to prepare a spore suspension. A 48-well multiplate was charged with 0.5 ml of each composition of Examples and Comparative Examples and 0.5 ml of GP medium. Further, 0.1 ml of the prepared spore suspension was added, and the cells were cultured at 28° C. for 1 week and then evaluated. As a control, 0.1 ml of spore suspension was added to 1 ml of GP medium, and culture and determination were performed in the same manner. The judgment was made based on the presence or absence of the development of the fungus by the naked eye. The results are shown in Table 1.

  From the above results, growth of Trichophyton was not observed in all Examples, whereas growth of Trichophyton was observed in Comparative Example 1 in which silver iodide as an active ingredient was not contained. From this, it was confirmed that the antifungal composition of the present invention has high antifungal properties.

(Moldproof test method)
As the fungi, black mold (Aspergillus niger), blue mold (Penicillium pinophilum), and black mold (Rhizopus oryzae) cultured in a PDA slant medium were used. 10 ml of sterilized water containing a wetting agent (0.005% sodium dioctyl sulfosuccinate aqueous solution) was placed in a test tube in which each bacterium was cultured, and spores were scraped off with a platinum loop. The obtained liquid was filtered with sterile gauze and adjusted with sterilized water containing a wetting agent so that the spores would be 10 ⁶ cells/ml, to prepare a spore suspension. A 48-well multiplate was charged with 0.5 ml of each composition of Example 4 and Comparative Example 4 and 0.5 ml of GP medium. Further, 0.1 ml of the prepared spore suspension was added, and the cells were cultured at 28° C. for 1 week and then evaluated. As a control, 0.1 ml of spore suspension was added to 1 ml of GP medium, and culture and determination were performed in the same manner. The judgment was made based on the presence or absence of the development of the fungus by the naked eye. The results are shown in Table 2.

  As can be understood from Table 1 and Table 2, in all of the examples used in the test, no growth was observed in any of the fungi, whereas in Comparative Examples not containing silver iodide as an active ingredient. Fungal growth was observed. From this, it was confirmed that the antifungal composition of the present invention has high antifungal properties.

(Moldproof test method (quantitative test))
As the fungus, Trichophyton mentagrophytes, Cladosporium cladosporioides, Penicillium pinophilum, and Rhizopus oryzae cultivated in a PDA slant medium were used. 10 ml of sterilized water containing a wetting agent (0.005% sodium dioctyl sulfosuccinate aqueous solution) was placed in a test tube in which each bacterium was cultured, and spores were scraped off with a platinum loop. The obtained liquid was filtered with sterile gauze and adjusted with sterilized water containing a wetting agent so that the spores would be 10 ⁶ cells/ml, to prepare a spore suspension. 0.1 ml of the spore suspension was mixed with 1.0 ml of each composition of Examples and Control, and stirred for a certain period of time. After each time, a 10-step dilution series was prepared with SCDLP medium. The viable cell count was measured by the plate culture method using PDA medium. The results are shown in Table 3.

  From the above results, it was confirmed that the number of viable bacteria was below the detection limit in 30 minutes for all fungi. From this, it was also confirmed that the antifungal composition of the present invention has a fungicidal effect.

Claims (2)

Nanoparticles composed of silver halide having a particle size of 1 nm or more and less than 10 nm,
A halide ion of the same element as the halogen constituting the silver halide,
At least one water-soluble polymer,
An organic acid having a carboxyl group and/or a salt thereof,
The molar ratio of silver and the halide ions in the silver halide is 1: 10 to 1: Ri 1000 der,
An antifungal composition characterized in that an electric double layer is formed around the silver halide nanoparticles .   The antifungal composition according to claim 1, further comprising at least one selected from the group consisting of a surfactant, a humectant, a solvent, and an antioxidant.