JP6693747B2 - 植物体中のたばこ特異的ニトロソアミンの低減 - Google Patents
植物体中のたばこ特異的ニトロソアミンの低減 Download PDFInfo
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
- A24B15/245—Nitrosamines
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Manufacture Of Tobacco Products (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP12198966 | 2012-12-21 | ||
EP12198966.9 | 2012-12-21 | ||
PCT/EP2013/077532 WO2014096283A2 (fr) | 2012-12-21 | 2013-12-19 | Réduction de nitrosamine spécifique du tabac dans les plantes |
Publications (3)
Publication Number | Publication Date |
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JP2016508032A JP2016508032A (ja) | 2016-03-17 |
JP2016508032A5 JP2016508032A5 (fr) | 2017-03-02 |
JP6693747B2 true JP6693747B2 (ja) | 2020-05-13 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2015548608A Active JP6693747B2 (ja) | 2012-12-21 | 2013-12-19 | 植物体中のたばこ特異的ニトロソアミンの低減 |
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US (1) | US10563215B2 (fr) |
EP (1) | EP2935588A2 (fr) |
JP (1) | JP6693747B2 (fr) |
KR (1) | KR102253223B1 (fr) |
CN (1) | CN105247053B (fr) |
AP (1) | AP2015008563A0 (fr) |
CA (1) | CA2894955C (fr) |
MX (2) | MX2015008084A (fr) |
PH (1) | PH12015501204A1 (fr) |
RU (1) | RU2735254C2 (fr) |
WO (1) | WO2014096283A2 (fr) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9980509B2 (en) | 2013-04-05 | 2018-05-29 | R.J. Reynolds Tobacco Company | Modification of bacterial profile of tobacco |
AP2016009665A0 (en) * | 2014-06-25 | 2016-12-31 | Philip Morris Products Sa | Modulation of nitrate content in plants |
GB201501941D0 (en) * | 2015-02-05 | 2015-03-25 | British American Tobacco Co | Method |
JP2018516550A (ja) * | 2015-05-05 | 2018-06-28 | ノース カロライナ ステート ユニバーシティNorth Carolina State University | タバコ中のタバコ特異的ニトロソアミンnnkを低減するための方法及び組成物 |
CN109715810B (zh) * | 2016-03-11 | 2023-07-14 | 奥驰亚客户服务有限公司 | 用于生产烟杈减少或消除的烟草植物和制品的组合物和方法 |
WO2019027861A1 (fr) | 2017-07-31 | 2019-02-07 | R. J. Reynolds Tobacco Company | Procédés et compositions pour l'édition de gènes à base de virus dans des plantes |
BR112020017500A2 (pt) | 2018-03-28 | 2020-12-22 | Philip Morris Products S.A. | Modulação do teor de aminoácidos em uma planta |
AR115023A1 (es) | 2018-03-28 | 2020-11-18 | Philip Morris Products Sa | Modulación del contenido de azúcar reductor en una planta |
US11976286B2 (en) | 2018-12-30 | 2024-05-07 | Philip Morris Products S.A. | Modulation of nitrate levels in plants via mutation of nitrate reductase |
BR112021024528A2 (pt) * | 2019-06-05 | 2022-01-18 | Reynolds Tobacco Co R | Fatores de transcrição bzip regulam a conversão de nicotina em nornicotina e reduzem os níveis de precursores específicos de tabaco (tsna) |
CN110499388B (zh) * | 2019-09-29 | 2023-03-24 | 云南省烟草农业科学研究院 | 鉴别烟草抗斑萎病位点rtsw等位基因类型的共显性标记引物组、鉴别方法及其应用 |
JP2022550383A (ja) | 2019-10-01 | 2022-12-01 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | 植物における還元糖含有量の調節(inv) |
CN114514321A (zh) | 2019-10-01 | 2022-05-17 | 菲利普莫里斯生产公司 | 调节植物中的糖和氨基酸含量(sultr3) |
CN111690661A (zh) * | 2020-06-01 | 2020-09-22 | 云南省烟草农业科学研究院 | 一种烟草NtbHLH13基因突变体及分子鉴定方法和应用 |
WO2023036691A1 (fr) | 2021-09-10 | 2023-03-16 | Philip Morris Products S.A. | Modulation des profils d'alcaloïdes chez nicotiana tabacum |
WO2023117661A1 (fr) | 2021-12-20 | 2023-06-29 | Philip Morris Products S.A. | Augmentation de l'anatabine dans le tabac par régulation de la méthyl-putrescine oxydase |
WO2023117701A1 (fr) | 2021-12-21 | 2023-06-29 | Philip Morris Products S.A. | Modulation de production de nicotine par modification de l'expression ou de la fonction de la nicotinamidase dans des plantes |
WO2023127723A1 (fr) * | 2021-12-27 | 2023-07-06 | 日本たばこ産業株式会社 | Plante de tabac |
WO2024079137A1 (fr) | 2022-10-13 | 2024-04-18 | Philip Morris Products S.A. | Augmentation de la biomasse de feuilles et de l'efficacité d'utilisation d'azote par régulation de ntp2 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5810020A (en) | 1993-09-07 | 1998-09-22 | Osmotek, Inc. | Process for removing nitrogen-containing anions and tobacco-specific nitrosamines from tobacco products |
US6202649B1 (en) | 1996-12-02 | 2001-03-20 | Regent Court Technologies | Method of treating tobacco to reduce nitrosamine content, and products produced thereby |
US6586661B1 (en) * | 1997-06-12 | 2003-07-01 | North Carolina State University | Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid |
KR100458405B1 (ko) | 1997-06-20 | 2004-11-26 | 리전트 코트 테크놀로지스 | 니트로스아민 함량을 감소시키기 위한 담배의 처리 방법 및 이에 의해 제조된 제품 |
US7700834B2 (en) * | 2001-11-13 | 2010-04-20 | U.S. Smokless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
RU2191529C1 (ru) | 2001-12-21 | 2002-10-27 | Романенко Александр Павлович | Устройство для курения |
WO2004098323A1 (fr) * | 2003-05-06 | 2004-11-18 | Japan Tobacco Inc. | Procédé de production de tabac régénéré |
JP4160868B2 (ja) * | 2003-07-10 | 2008-10-08 | 日本たばこ産業株式会社 | 葉タバコ中のニトロソアミン含量の低減方法 |
AP2265A (en) * | 2003-08-19 | 2011-08-01 | 22Nd Century Ltd Llc | Reduced-exposure tobacco products. |
ATE530656T1 (de) | 2005-02-23 | 2011-11-15 | Univ North Carolina State | Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene. |
WO2008070274A2 (fr) | 2006-10-13 | 2008-06-12 | North Carolina State University | Modification de la teneur en alcaloïde du tabac par modification de gènes de cytochrome p450 spécifique |
RU2324737C1 (ru) * | 2006-10-18 | 2008-05-20 | Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) | Способ получения трансгенных растений табака с повышенным содержанием пролина |
EP2152891A4 (fr) | 2007-06-06 | 2010-09-22 | Monsanto Technology Llc | Gènes et leurs utilisations pour l'amélioration des plantes |
BRPI0820042B1 (pt) | 2007-11-12 | 2020-05-19 | North Carolina State University | método de obtenção de uma planta de tabaco, ou célula ou parte desta, tendo níveis reduzidos de nornicotina, produto de tabaco, método para fabricar um produto de tabaco, polinucleotídeo isolado, cassete de expressão, polipetpídeo isolado e método de obtenção de uma planta, ou parte de planta desta, do gênero nicotiana |
PL2231861T3 (pl) | 2007-12-13 | 2015-04-30 | Philip Morris Products Sa | Rośliny transgeniczne modyfikowane w celu zmniejszenia transportu kadmu, produkty pochodne i metody związane |
WO2009091518A2 (fr) * | 2008-01-15 | 2009-07-23 | Monsanto Technology, Llc | Nouvelles molécules d'acide nucléique et de protéine de maïs isolées et procédés d'utilisation de ces molécules pour produire une plante transgénique présentant des caractéristiques agronomiques améliorées |
CA2777802A1 (fr) * | 2009-10-22 | 2011-04-28 | Basf Plant Science Company Gmbh | Plantes presentant des traits lies au rendement ameliores et procede pour les obtenir |
EA033565B1 (ru) | 2010-01-15 | 2019-11-05 | Univ North Carolina State | Композиции и способы, предназначенные для минимизации синтеза норникотина в растениях табака |
US9528118B2 (en) | 2010-09-03 | 2016-12-27 | Philip Morris Products S.A. | Heavy metal reduction in planta |
-
2013
- 2013-12-19 WO PCT/EP2013/077532 patent/WO2014096283A2/fr active Application Filing
- 2013-12-19 US US14/653,924 patent/US10563215B2/en active Active
- 2013-12-19 AP AP2015008563A patent/AP2015008563A0/xx unknown
- 2013-12-19 KR KR1020157018188A patent/KR102253223B1/ko active IP Right Grant
- 2013-12-19 EP EP13817681.3A patent/EP2935588A2/fr active Pending
- 2013-12-19 MX MX2015008084A patent/MX2015008084A/es unknown
- 2013-12-19 JP JP2015548608A patent/JP6693747B2/ja active Active
- 2013-12-19 CN CN201380073174.4A patent/CN105247053B/zh active Active
- 2013-12-19 RU RU2015126909A patent/RU2735254C2/ru active
- 2013-12-19 CA CA2894955A patent/CA2894955C/fr active Active
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US20150315603A1 (en) | 2015-11-05 |
CA2894955A1 (fr) | 2014-06-26 |
CN105247053B (zh) | 2020-07-31 |
JP2016508032A (ja) | 2016-03-17 |
MX2015008084A (es) | 2016-03-31 |
RU2735254C2 (ru) | 2020-10-29 |
RU2015126909A (ru) | 2017-01-27 |
US10563215B2 (en) | 2020-02-18 |
KR20150096451A (ko) | 2015-08-24 |
CA2894955C (fr) | 2023-10-31 |
PH12015501204A1 (en) | 2015-08-17 |
CN105247053A (zh) | 2016-01-13 |
MX2019001598A (es) | 2019-07-01 |
EP2935588A2 (fr) | 2015-10-28 |
WO2014096283A3 (fr) | 2014-08-14 |
WO2014096283A2 (fr) | 2014-06-26 |
AP2015008563A0 (en) | 2015-06-30 |
KR102253223B1 (ko) | 2021-05-21 |
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