JP6691558B2 - 改善された活性を持つ新規のｐ４５０−ｂｍ３バリアント - Google Patents

改善された活性を持つ新規のｐ４５０−ｂｍ３バリアント Download PDF

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JP6691558B2
JP6691558B2 JP2017567368A JP2017567368A JP6691558B2 JP 6691558 B2 JP6691558 B2 JP 6691558B2 JP 2017567368 A JP2017567368 A JP 2017567368A JP 2017567368 A JP2017567368 A JP 2017567368A JP 6691558 B2 JP6691558 B2 JP 6691558B2
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sequence
polypeptide
amino acid
host cell
polynucleotide
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JP2018520674A (ja
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ロバート オズボーン，
ロバート オズボーン，
ヴェスナ ミッチェル，
ヴェスナ ミッチェル，
キン ユ ナイン トゥエ，
キン ユ ナイン トゥエ，
シユン ザン，
シユン ザン，
エリカ エム． ミルチェク，
エリカ エム． ミルチェク，
ジェフリー シー． ムーア，
ジェフリー シー． ムーア，
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コデクシス， インコーポレイテッド
コデクシス， インコーポレイテッド
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14001—Unspecific monooxygenase (1.14.14.1)

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  • Health & Medical Sciences (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2017567368A 2015-07-07 2016-06-02 改善された活性を持つ新規のｐ４５０−ｂｍ３バリアント Active JP6691558B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562189281P 2015-07-07 2015-07-07
US62/189,281 2015-07-07
PCT/US2016/035399 WO2017007547A1 (en) 2015-07-07 2016-06-02 Novel p450-bm3 variants with improved activity

Publications (3)

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JP2018520674A JP2018520674A (ja) 2018-08-02
JP2018520674A5 JP2018520674A5 (https=) 2019-04-18
JP6691558B2 true JP6691558B2 (ja) 2020-04-28

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US (7) US9683220B2 (https=)
EP (1) EP3319987B1 (https=)
JP (1) JP6691558B2 (https=)
CN (1) CN108271374B (https=)
CA (1) CA2991195C (https=)
DK (1) DK3319987T3 (https=)
IL (1) IL256441B (https=)
WO (1) WO2017007547A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9708587B2 (en) * 2014-07-09 2017-07-18 Codexis, Inc. P450-BM3 variants with improved activity
US9683220B2 (en) * 2015-07-07 2017-06-20 Codexis, Inc. P450-BM3 variants with improved activity
ES2892972T3 (es) * 2015-11-02 2022-02-07 Univ Texas Métodos de activación de CD40 y bloqueo de punto de control inmunitario
US11591627B2 (en) 2018-03-21 2023-02-28 University Of Florida Research Foundation, Incorporated Modified bacteria for production of nitroaromatics
EP4622999A1 (en) * 2022-11-22 2025-10-01 Codexis, Inc. P450-bm3 variants with improved activity
CN120813367A (zh) * 2023-03-20 2025-10-17 科德克希思公司 具有改进的活性的p450-bm3变体

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
FI964808A0 (fi) 1994-06-03 1996-12-02 Novo Nordisk Biotech Inc Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
US5483345A (en) 1994-09-06 1996-01-09 Mrs Technology, Inc. Alignment system for use in lithography utilizing a spherical reflector having a centered etched-on projection object
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
GB2306485B (en) * 1995-11-01 1998-12-09 British Gas Plc Enzyme mutant
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
KR100570935B1 (ko) 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
US6537746B2 (en) 1997-12-08 2003-03-25 Maxygen, Inc. Method for creating polynucleotide and polypeptide sequences
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
ES2341217T3 (es) 1999-01-19 2010-06-17 Maxygen, Inc. Recombinacion de acidos nucleicos mediada por oligonucleotidos.
AU3391900A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Encryption of traits using split gene sequences
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
EP1272967A2 (en) 2000-03-30 2003-01-08 Maxygen, Inc. In silico cross-over site selection
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
JP5319865B2 (ja) 2002-03-01 2013-10-16 コデクシス メイフラワー ホールディングス， エルエルシー 機能的生体分子を同定する方法、システム、およびソフトウェア
EP1488335A4 (en) 2002-03-09 2006-11-15 Maxygen Inc OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION
EP1639091B1 (en) * 2003-06-17 2012-12-05 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
GB0403992D0 (en) * 2004-02-23 2004-03-31 Isis Innovation Oxidation by hydrogen peroxide
CN1273585C (zh) * 2004-11-29 2006-09-06 浙江大学 细胞色素 p450bm－3 单加氧酶变体基因及其用途
JP2009514969A (ja) * 2005-11-09 2009-04-09 コンビナトアールエックス インコーポレーティッド 医学的状態を治療するための方法、組成物、およびキット
PT2068938E (pt) * 2006-09-22 2011-03-23 Novartis Ag Optimização do tratamento da leucemia filadélfia positiva com o inibidor de tirosina quinase abl imatinib
JP2010504978A (ja) * 2006-09-29 2010-02-18 アイデニクス ファーマシューティカルズ，インコーポレーテッド Ｈｉｖ阻害剤としての鏡像異性的に純粋なホスホインドール
US8101399B2 (en) 2006-12-12 2012-01-24 The Regents Of The University Of California Artemisinic epoxide and methods for producing same
US20080268517A1 (en) * 2007-02-08 2008-10-30 The California Institute Of Technology Stable, functional chimeric cytochrome p450 holoenzymes
US8802401B2 (en) * 2007-06-18 2014-08-12 The California Institute Of Technology Methods and compositions for preparation of selectively protected carbohydrates
GB0719620D0 (en) * 2007-10-08 2007-11-14 Isis Innovation Mutant Enzymes
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
DK3023494T3 (en) 2008-06-13 2019-02-25 Codexis Inc PROCEDURE FOR SYNTHESIS OF POLYNUCLEOTIDE VARIETIES
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
EP2426198A1 (en) * 2010-09-03 2012-03-07 B.R.A.I.N. Biotechnology Research And Information Network AG Cytochrome P450 monooxygenase variants
WO2012109586A2 (en) * 2011-02-11 2012-08-16 University Of Rochester Methods and systems for evaluating and predicting the reactivity of monooxygenase enzymes
WO2013076258A2 (en) * 2011-11-23 2013-05-30 Dsm Ip Assets B.V. P450 bm3 mutants and their use for regio- and stereoselective hydroxylation of alpha-and beta-ionone
CN102757944A (zh) * 2012-07-24 2012-10-31 浙江大学宁波理工学院 细胞色素p450bm-3(d168l/e435t)变体酶及其编码基因和用途
JP2015534464A (ja) * 2012-10-09 2015-12-03 カリフォルニア インスティチュート オブ テクノロジー ヘム酵素によって触媒されるインビボおよびインビトロオレフィンシクロプロパン化
US9708587B2 (en) * 2014-07-09 2017-07-18 Codexis, Inc. P450-BM3 variants with improved activity
US9683220B2 (en) * 2015-07-07 2017-06-20 Codexis, Inc. P450-BM3 variants with improved activity

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Publication number Publication date
US20170247667A1 (en) 2017-08-31
CA2991195A1 (en) 2017-01-12
US10450550B2 (en) 2019-10-22
DK3319987T3 (da) 2021-06-21
US20190024059A1 (en) 2019-01-24
EP3319987A4 (en) 2018-12-19
JP2018520674A (ja) 2018-08-02
US20230250403A1 (en) 2023-08-10
US10113153B2 (en) 2018-10-30
EP3319987B1 (en) 2021-05-05
US10982197B2 (en) 2021-04-20
US20200002685A1 (en) 2020-01-02
WO2017007547A1 (en) 2017-01-12
US11591578B2 (en) 2023-02-28
US20200291365A1 (en) 2020-09-17
IL256441A (en) 2018-02-28
EP3319987A1 (en) 2018-05-16
IL256441B (en) 2020-02-27
CA2991195C (en) 2021-06-29
US20210214694A1 (en) 2021-07-15
CN108271374A (zh) 2018-07-10
US20170009213A1 (en) 2017-01-12
CN108271374B (zh) 2021-09-21
US9683220B2 (en) 2017-06-20
US10704030B2 (en) 2020-07-07

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