JP6690143B2 - Detector and analyzer - Google Patents

Description

  The present invention relates to a detector used for measuring the concentration of a specific component contained in a sample solution and an analyzer using this detector.

  The light (absorbance) absorbed by a specific component is measured by irradiating a sample solution (sample solution) obtained by reacting a sample such as a biological sample with a reagent with light of a specific wavelength and measuring the amount of transmitted light (light amount). A technique for calculating and detecting the concentration of a specific component contained in a sample is known.

  In Patent Document 1, the respective lights emitted from a plurality of light sources that emit different wavelengths are respectively condensed through different condenser lenses, and the condensed respective lights are reflected by a corresponding half mirror to be the same. Techniques for collecting on the optical path and leading to a sample solution are disclosed.

JP, 9-236606, A

  In the technique disclosed in Patent Document 1, the condensed light is reflected by the corresponding half mirrors and collected on the same optical path, so that the light amount is reduced. Here, in order to secure the amount of light with which the sample solution is irradiated, it is necessary to increase the light output, which causes problems such as an increase in power consumption and an increase in the size of a device power supply unit for securing power.

  In view of the above facts, the present invention has an object to provide a detection device that can suppress a decrease in the amount of light when condensing light emitted from a plurality of light sources, and an analysis device that uses this detection device. To do.

The detection device according to the first aspect of the present invention includes a plurality of light emitting diodes that emit light of different wavelengths and a plurality of light beams emitted from the plurality of light emitting diodes arranged in parallel on one condenser lens or one optical axis. The first optical system that collects light through a plurality of condensing lenses arranged at the same time and guides it to a light-transmitting container in which the sample solution is stored; A second optical system that guides the different light beams to a plurality of light receiving elements, respectively.

In the detection device according to the first aspect of the present invention , the first optical system allows the respective lights emitted from the plurality of light emitting diodes (light sources) to be one condensing lens or a plurality of collecting lenses arranged in parallel on one optical axis. It is condensed through an optical lens and guided to a container in which the sample solution is stored. Therefore, the detection device has a plurality of light-emitting diodes as compared with, for example, a device in which light emitted from a plurality of light sources is reflected by a half mirror and is collected on the same optical path to be guided to a container in which a sample solution is stored. It is possible to suppress a decrease in the amount of light when collecting the respective lights emitted from the.

  Further, the light transmitted through the container is dispersed by the second optical system, and the dispersed light is guided to the plurality of light receiving elements as light having different wavelengths. These light receiving elements measure (detect) the amount of received light. When the specific component in the sample solution or the substance generated by conversion from the specific component absorbs the light of the specific wavelength, the amount of light measured by the light receiving element that receives the light of the specific wavelength decreases. Therefore, the concentration of the specific component contained in the sample can be calculated.

A detection device according to a second aspect of the present invention is the detection device according to the first aspect of the present invention , wherein the plurality of light emitting diodes are arranged in a matrix on a substrate.

In the detection device according to the second aspect of the present invention, since the plurality of light emitting diodes are arranged in a matrix on the substrate, for example, the emitted light is compared to the case where the plurality of light sources are arranged in a line on the substrate. The size (outer diameter) of the condenser lens that receives and condenses the light can be reduced. As a result, the size of the device can be reduced.

An analysis apparatus according to a third aspect of the present invention includes a base plate, a disk-shaped reaction table that is provided above the base plate and is rotatable with respect to the base plate about a rotation axis that extends in the vertical direction, and the reaction. A plurality of containers, which are provided on the outer peripheral portion of the table at intervals in the circumferential direction of the reaction table and have the light transmissive property for accommodating the sample solution , and the first aspect or the first aspect of the present invention installed on the base plate . A detection device according to two aspects , wherein the first optical system of the detection device is arranged closer to the rotation shaft than the container of the reaction table, and the second optical system of the detection device is the reaction device. The container is arranged in the peripheral portion of the table, and the container passes on the optical path from the first optical system to the second optical system.

In the analysis apparatus of the third aspect of the present invention, the detection apparatus of the first aspect or the second aspect of the present invention is installed on the base plate. In addition, the first optical system of the detection device is arranged on the rotation axis side of the container of the reaction table, and the second optical system is arranged in the peripheral portion of the reaction table, so that light from the first optical system to the second optical system is arranged. It is configured such that a light-transmissive container containing the sample solution passes on the path. Accordingly, when the reaction table is rotated, the plurality of containers sequentially pass on the optical path from the first optical system to the second optical system, so that, for example, the container containing the sample solution is transferred from the first optical system to the second optical system each time. Compared with the configuration in which the light is conveyed on the optical path to the optical system, the light condensed by the first optical system can be efficiently applied to the sample solution contained in the plurality of containers. Thereby, the concentration of the specific component contained in the sample can be efficiently calculated.

An analysis apparatus according to a fourth aspect of the present invention is the analysis apparatus according to the third aspect of the present invention , wherein the first optical system has an optical path of light condensed through one or more condenser lenses of the base plate. It is an optical system that extends upward and is directed in a horizontal direction with respect to the base plate by a reflection mirror.

In the analysis device of the fourth aspect of the present invention, the optical path of the light condensed through one or a plurality of condenser lenses is extended toward the upper side of the base plate, and the reflection mirror (total reflection mirror) is arranged in the horizontal direction with respect to the base plate. The width of the device (the length along the horizontal direction) is narrower than that in the case where the optical path of the light condensed through one or more condenser lenses is extended in the horizontal direction. it can.

  ADVANTAGE OF THE INVENTION According to this invention, the detection apparatus which can suppress the fall of the light amount at the time of condensing each light emitted from a some light source, and the analyzer which uses this detection apparatus can be provided.

It is a top view showing a schematic structure of an analysis device concerning one embodiment of the present invention. It is sectional drawing (2-2 sectional view taken on the line of FIG. 1) of the detection apparatus which concerns on one Embodiment of this invention. FIG. 3 is a plan view (a plan view seen from the direction of arrow 3 in FIG. 2) showing an arrangement pattern of light emitting diodes used in the detection device. It is a flow chart for explaining the analysis procedure of the sample using the analysis device of the present invention.

  Hereinafter, a detection device 70 according to an embodiment of the present invention and an analysis device 10 using the detection device 70 will be described with reference to the drawings. In the present embodiment, an example will be described in which blood is used as a sample, a glycated hemoglobin (HbA1c) concentration is measured by an enzyme method, and a glucose concentration is measured by an electrode method.

[Overall schematic configuration of analyzer]
As shown in FIG. 1, the analyzer 10 mainly includes a sample rack area 12, a sample dispensing area 14, a reaction area 16, a reagent dispensing area 18, a detection area 20, and a washing area 22, And an electrode measurement area 24.

  A plurality of (five in the present embodiment) sample racks 26 are arranged in the sample rack area 12, and the sample rack 26 holds five sample containers 28 each containing a sample. Further, the sample racks 26 sequentially move one by one along the arrow A direction in FIG. The position of the moving sample rack 26 is detected by a position detection sensor (not shown). A barcode (not shown) is attached to each of the sample containers 28, and the sample can be identified by reading the barcode with a barcode reader or the like.

  The sample dispensing area 14 is provided with a sample dispensing device 30 having a sample dispensing pipette 36, and a pipette cleaning unit 32 for cleaning the tip of the sample dispensing pipette 36. The sample dispensing device 30 includes a rotating arm 34 having a sample dispensing pipette 36 attached to the tip thereof. The rotating arm 34 can be horizontally swung about a rotating shaft 34A and can be vertically moved up and down by a drive mechanism (not shown). By rotating the rotating arm 34, the sample pipette 36 moves between the sample container 28 in the sample rack area 12 and the reaction container 42 set in the reaction table 38 in the reaction area 16 described later. .

  Further, the sample dispensing pipette 36 includes a suction / exhaust device (not shown) that performs vacuum suction of the sample and discharge of the vacuum-sucked sample, and the sample suctioned from the sample container 28 can be dispensed into the reaction container 42. There is. Further, the sample dispensing pipette 36 communicates with a purified water tank (not shown) in which purified water is stored, and the aspirated sample and purified water are discharged into the reaction container 42 so that the sample is stored in the reaction container 42. Can be diluted.

  The reaction area 16 is provided with a reaction table 38 and a stirring unit 40 arranged around the reaction table 38. The reaction table 38 has a disk shape, and a plurality of (30 in the present embodiment) reaction containers 42 having light transmittance are provided on the outer peripheral portion at intervals along the circumferential direction. The reaction container 42 is a quadrangular prism-shaped cell having an open upper surface, and is formed of heat-resistant glass as an example in the present embodiment. Note that the present invention is not limited to this configuration, and the reaction container 42 may be formed of resin (for example, plastic) as long as it has a light-transmitting property.

  The reaction table 38 is arranged above the base plate 39 (see FIG. 2) and is rotatable with respect to the base plate 39 about the rotation shaft 38A. The rotary shaft 38A extends in the vertical direction and receives the driving force from a driving mechanism (not shown) to rotate integrally with the reaction table 38. At this time, the reaction container 42 also rotates together with the rotation of the reaction table 38. A temperature adjusting device (not shown) is provided in the reaction table 38, and the reaction container 42 is maintained (incubated) at a predetermined temperature (for example, 37 ° C.) by the temperature adjusting device.

  The stirring unit 40 includes a stirring rod 44 driven by a driving mechanism (not shown). By inserting the stirring rod 44 into the reaction container 42 and vibrating it, the sample in the reaction container 42 is stirred.

  In the reagent dispensing area 18, a reagent container housing portion 46, a reagent dispensing unit 50 having a first reagent dispensing pipette 62 and a second reagent dispensing pipette 64, a first reagent dispensing pipette 62 and a second reagent. A pipette cleaning unit 52 for cleaning the tip of the dispensing pipette 64 is provided.

  The reagent container storage portion 46 is provided with a plurality of recesses 46A, and the recesses 46A hold a plurality of reagent containers 54. In the present embodiment, R1 is used as the first reagent and R2 is used as the second reagent, and two R1 containers 54A containing R1 and two R2 containers 54B containing R2 are provided in the recesses 46A. Held in.

  The reagent dispensing unit 50 includes a first drive arm 58 and a second drive arm 60. A first reagent dispensing pipette 62 is attached to the first drive arm 58, and the first reagent dispensing pipette 62 is mounted on the R1 container 54A, the pipette washing section 52, and the reaction container 42 of the reaction table 38. It is possible to move between them. A second reagent dispensing pipette 64 is attached to the second drive arm 60. The second reagent dispensing pipette 64 is mounted on the R2 container 54B, the pipette washing section 52, and the reaction container 42 of the reaction table 38. The first reagent dispensing pipette 62 and the upper portion can be moved together.

  The first reagent dispensing pipette 62 and the second reagent dispensing pipette 64 are arranged in parallel along the moving direction of the reaction container 42 (the rotation direction of the reaction table 38). Further, the first reagent dispensing pipette 62 and the second reagent dispensing pipette 64 are equipped with a suction / discharge device (not shown) for vacuum suction of the reagent and discharge of the vacuum suctioned reagent. It is possible to dispense into the reaction container 42.

  A detection device 70 is provided in the detection area 20. As shown in FIG. 2, the detection device 70 includes an LED light source unit 65 including a plurality of light emitting diodes (hereinafter, referred to as “LEDs”) 76 that emit light having different wavelengths, and light having different wavelengths. And a light receiving section 66 having a plurality of light receiving elements 96 for receiving the light. The LED light source unit 65 is arranged below the reaction table 38, and the light receiving unit 66 is arranged around the reaction table 38. Light is emitted from the LED light source unit 65 toward the side surface of the reaction container 42, and the reaction container 42. The light transmitted through the sample solution L therein is received by the light receiving unit 66. The light receiving unit 66 measures the amount of light received (absorbance). Then, the glycated hemoglobin (HbA1c) concentration in the sample is calculated based on the absorbance measured by the light receiving unit 66.

  The cleaning area 22 is provided with a plurality of cleaning nozzles 22A that are driven in the vertical direction by a drive mechanism (not shown). The cleaning nozzle 22A communicates with a cleaning liquid tank (not shown) in which the cleaning liquid is stored, and discharges the cleaning liquid into the reaction container 42 to clean the inside of the reaction container 42 and then sucks the cleaning liquid.

  The electrode measuring area 24 is provided with a measuring device 24A having an electrode (not shown). The sample (blood) in the sample container 28 is dispensed into the measuring device 24A by the sample dispensing pipette 36 of the sample dispensing device 30. In the measuring device 24A, glucose in the dispensed blood reacts with glucose oxidase (glucose oxidase) in the presence of water and oxygen molecules to generate hydrogen peroxide, and the generated hydrogen peroxide is generated on the electrode surface. The glucose concentration can be calculated by measuring the amount of current when the glucose is decomposed.

[Configuration of detector]
Next, the configuration of the detection device 70 will be described with reference to FIGS. 2 and 3.

  As shown in FIG. 2, the detection device 70 includes an LED light source unit 65 and a light receiving unit 66. The LED light source unit 65 includes a housing 65A. The housing 65A is installed on the base plate 39. An opening 65B is formed at the upper part of the housing 65A so as to open radially outward of the reaction table 38. 1 and 2, the radial direction of the reaction table 38 is indicated by an arrow X. Here, the inner side in the radial direction of the reaction table 38 refers to the side directed toward the rotation axis 38A (see FIG. 1) along the radial direction, and the outer side in the radial direction of the reaction table 38 is the opposite side to the inner side in the radial direction, that is, , The side away from the rotation axis 38A in the radial direction.

  Further, the light receiving unit 66 includes a housing 66A. The housing 66A is installed in the peripheral portion of the reaction table 38. An opening 66B is formed in the upper part of the housing 66A so as to open inward in the radial direction of the reaction table 38.

  Here, the light guided by the first optical system 72, which will be described later, is applied to the sample solution L in the reaction container 42 through the opening 65B of the housing 65A, and the light transmitted through the sample solution L has the opening. The light enters from 66B into the housing 66A, is dispersed by the second optical system 74, is converted into light having different wavelengths by a plurality of bandpass filters 95 described later, and is guided to a plurality of light receiving elements 96. That is, the first optical system 72 (LED light source unit 65) and the second optical system 74 (light receiving unit 66) are arranged so that the reaction container 42 passes on the optical path OL1 from the first optical system 72 to the second optical system 74. And are arranged on the base plate 39.

  A plurality of (four in the present embodiment) LEDs 76 having different wavelengths are provided in the housing 65A of the LED light source unit 65. As shown in FIG. 3, among the four LEDs 76, the LED 76A is configured to emit light with a wavelength of 570 nm. The LED 76B is configured to emit light with a wavelength of 660 nm, the LED 76C is configured to emit light with a wavelength of 700 nm, and the LED 76D is configured to emit light with a wavelength of 800 nm. Although the wavelengths of the LEDs 76A to 76D are set as described above in the present embodiment, the present invention is not limited to this configuration. The wavelengths of the LEDs 76A to 76D may be adjusted appropriately. Further, in the present embodiment, four LEDs 76 having different wavelengths are used, but the present invention is not limited to this configuration. Five or more LEDs 76 having different wavelengths may be used, or two or three LEDs 76 having different wavelengths may be used.

  These light emitting diodes 76A to 76D are arranged in a matrix on a single substrate 77. The substrate 77 is attached to the bottom surface of the housing 65A using an attachment member (not shown).

As shown in FIG. 2, a condensing optical system 78 is arranged above the light emitting diodes 76A to 76D in the housing 65A of the LED light source unit 65. The condensing optical system 78 is an optical system that condenses each light emitted from the light emitting diodes 76A to 76D, and makes the condensing lens 80 and the light condensed by the condensing lens 80 parallel light. One or a plurality of optical systems each including a plano-concave lens 82 as a set are arranged in parallel. Further, the respective lenses forming the condensing optical system 78 are arranged in parallel on one optical axis. In other words, the lenses forming the condensing optical system 78 are arranged so that their optical axes coincide with each other. In this embodiment, each lens is arranged so that the optical axis of the condensing optical system 78 extends in the vertical direction. Therefore, the optical path OL2 of the light condensed through the condensing optical system 78 extends upward in the base plate 39.
The condensing optical system 78 of the present embodiment is configured by vertically arranging two optical systems each including one condensing lens 80 and one plano-concave lens 82 as a set. Not limited. For example, the condensing optical system may be configured by arranging three or more optical systems, each including one condensing lens 80 and one plano-concave lens 82 as a set, vertically. Further, an optical system in which one condensing lens 80 and one plano-concave lens 82 are combined may be a condensing optical system. In the condensing optical system 78, it is preferable that the condensing lenses 80 and the plano-concave lenses 82 are provided in the same number.

  Further, in the condensing optical system 78 of the present embodiment, each light emitted from the LEDs 76A to 76D is condensed by the condensing lens 80A, and the condensed light is collimated by the plano-concave lens 82A. Then, the parallel light from the plano-concave lens 82A is further condensed by the condenser lens 80B and converted into parallel light by the plano-concave lens 82B. At this time, the diameter of the light passing through the plano-concave lens 82B becomes smaller than the diameter (light diameter) of the light passing through the plano-concave lens 82A. The light passing through the condensing optical system 78 is condensed and the diameter of the light is reduced. In other words, the optical axis cross section becomes smaller. In this embodiment, as shown in FIG. 2, one of the two condenser lenses 80 located on the LED side is referred to as a condenser lens 80A, and the other is referred to as a condenser lens 80B. Further, of the two plano-concave lenses 82, the one located on the LED side is referred to as a plano-concave lens 82A, and the other is referred to as a plano-concave lens 82B.

A reflection mirror 84 that reflects the light condensed by the condensing optical system 78 toward the opening 65B is disposed above the condensing optical system 78. The reflection mirror 84 bends the optical path of the light condensed by the condensing optical system 78 in the horizontal direction with respect to the base plate 39, and guides it to the sample solution L in the reaction container 42. The condensing optical system 78 and the reflecting mirror 84 form the first optical system 72 of the LED light source unit 65. The first optical system 72 of the present embodiment is an example of the first optical system of the present invention.
Further, the respective lenses and mirrors forming the first optical system 72 of the present embodiment are attached to the housing 65A by attaching members (not shown).

The second optical system 74 is arranged in the housing 66 </ b> A of the light receiving unit 66. The second optical system 74 passes through a condensing lens 86 that condenses the light transmitted through the sample solution L, a collimator lens 88 that collimates the light condensed by the condensing lens 86, and a collimator lens 88. And a spectroscopic optical system 92 for spectroscopically dividing the light reflected by the specular mirror 90 into a plurality of (four in the present embodiment). There is.
The lenses and the mirrors forming the second optical system 74 of the present embodiment are attached to the housing 66A by attaching members (not shown). Moreover, the reflection mirror in the present embodiment is a total reflection mirror.

  The spectroscopic optical system 92 reflects a part of the light reflected by the reflection mirror 90 and transmits the rest, and a half mirror 92A that reflects a part of the light reflected by the half mirror 92A and transmits the rest. A half mirror 92B that guides the light reflected by the half mirror 92B to the light receiving element 96B through the bandpass filter 95A, and a reflection mirror 92C that guides the light reflected by the half mirror 92B to the light receiving element 96B through the bandpass filter 95B. Further, the spectroscopic optical system 92 reflects a light that has passed through the half mirror 92A and a reflection mirror 92D that reflects a part of the light reflected by the reflection mirror 92D and allows the rest to pass through and a light receiving element through a bandpass filter 95C. The half mirror 92E that guides the light to the 96C and the reflection mirror 92F that guides the light reflected by the half mirror 92E to the light receiving element 96D through the bandpass filter 95D. Further, in the spectroscopic optical system 92 of the present embodiment, the light reflected by the reflection mirror 90 is substantially evenly dispersed by each combination of the half mirrors 92A, 92B, 92E and the reflection mirrors 92C, 92D, 92F. The light can be guided to the respective light receiving elements 96A to 96D through the filters 95A to 95D.

  The bandpass filter 95A is configured to pass the wavelength of light emitted from the LED 76A. The bandpass filter 95B is configured to pass the wavelength of the light emitted from the LED 76B, the bandpass filter 95C is configured to pass the wavelength of the light emitted from the LED 76C, and the bandpass filter 95D. Is configured to pass the wavelength of light emitted from the LED 76D.

  The light beams having different wavelengths that have passed through the bandpass filters 95A to 96D are received by the four light receiving elements 96A to 96D, respectively. The four light receiving elements 96A to 96D of the present embodiment have the same structure. Further, these light receiving elements 96A to 96D are arranged in a row on one substrate 97. The board 97 is attached to the housing 66A by an attachment member (not shown). As the light receiving element 96, for example, a photodiode can be used.

  Further, the four light receiving elements 96A to 96D are arranged in parallel in the vertical direction on the substrate 97 extending in the vertical direction. For this reason, the respective mirrors forming the spectroscopic optical system 92 are arranged vertically. With this configuration, the width of the housing 66A is narrowed.

[Analysis procedure by analyzer]
Next, the procedure for analyzing blood as a sample (sample) by the analyzer 10 according to the embodiment of the present invention will be described according to the flowchart shown in FIG.

  First, in step 100 (S100), as a standby operation, the sample rack 26 holding the sample container 28 containing the sample (blood) is placed in the sample rack area 12.

  In step 102 (S102), the pipette cleaning unit 32 cleans the sample dispensing pipette 36, the pipette cleaning unit 52 cleans the first reagent dispensing pipette 62 and the second reagent dispensing pipette 64, and the cleaning nozzle 22A. The inside of the reaction container 42 is washed by. Further, in step 104 (S104), purified water is dispensed into the reaction container 42, and the absorbance of the purified water is measured in the detection area 20.

  Next, in step 106 (S106), the first drive arm 58 is moved in the vertical direction, and the first reagent dispensing pipette 62 sucks R1 in the R1 container 54A arranged in the recess 46A. Then, the first drive arm 58 and the second drive arm 60 are moved horizontally to move the first reagent dispensing pipette 62 onto the reaction container 42 of the reaction table 38. Then, the first drive arm 58 lowers the first reagent dispensing pipette 62 to dispense the sucked R1 into the reaction container 42.

  Next, in step 108 (S108), the rotation arm 34 is driven to move the sample dispensing pipette 36 to the sample rack area 12, and the sample in the sample container 28 is sucked by the sample dispensing pipette 36, and at the same time, purification is performed. Aspirate purified water from the water tank. After the rotation arm 34 is driven to move the sample dispensing pipette 36 onto the reaction table 38, the sucked sample and purified water are placed in a reaction container 42 different from the reaction container 42 in step 106 (S106). After discharging, the specimen is diluted with purified water in the reaction container 42 to cause hemolysis. Then, the diluted sample is sucked by the sample pipette 36.

  Next, the reaction table 38 is rotated to move the reaction container 42 in which R1 is dispensed to just below the sample dispensing pipette 36. Then, in step 110 (S110), the diluted sample sucked by the sample dispensing pipette 36 is dispensed into the reaction container 42 in which R1 is dispensed. The sample to be aspirated and dispensed is identified by the barcode.

  Next, in step 112 (S112), the diluted sample and R1 in the reaction container 42 are stirred by the stirring rod 44, and in step 114 (S114), the reaction container 42 is kept at a predetermined temperature for a predetermined time (for example, 5 minutes). Hold (for example, 37 ° C.). As a result, the sample blood and R1 react (primary reaction). At this time, in the detection area 20, the total hemoglobin (Hb) concentration is calculated by measuring the absorbance of the reaction solution (primary reaction solution) obtained by the primary reaction a plurality of times (for example, 10 times every 30 seconds). .

  Next, in step 116 (S116), the second drive arm 60 is moved in the vertical direction, and the second reagent dispensing pipette 64 sucks R2 in the R2 container 54B arranged in the recess 46A. Then, the first drive arm 58 and the second drive arm 60 are integrally moved in the horizontal direction, and the second reagent dispensing pipette 64 is moved onto the reaction container 42 of the reaction table 38.

  At the same time, the reaction table 38 is rotated to move the reaction container 42 containing the reaction liquid (primary reaction liquid) of the primary reaction between the diluted sample and R1 to directly below the second reagent dispensing pipette 64. Thereafter, the second drive arm 60 lowers the second reagent dispensing pipette 64 to dispense the sucked R2 into the reaction container 42 containing the primary reaction solution.

  Next, in step 118 (S118), the primary reaction liquid and R2 in the reaction container 42 are stirred by the stirring rod 44, and in step 120 (S120), the reaction container 42 is kept for a predetermined time (for example, 5 minutes) for a predetermined time. Hold at temperature (eg 37 ° C.). As a result, the sample blood and R2 react (secondary reaction). At this time, the glycated hemoglobin (HbA1c) concentration is determined by measuring the absorbance of the reaction solution (secondary reaction solution) obtained by the secondary reaction a plurality of times (for example, 10 times every 30 seconds) in the detection area 20. calculate.

  Then, in step 122 (S122), the inside of the reaction container 42 is cleaned by the cleaning nozzle 22A. From the total hemoglobin (Hb) concentration and the glycated hemoglobin (HbA1c) concentration calculated by the above procedure, the ratio of the glycated hemoglobin (HbA1c) in the total hemoglobin (Hb) can be calculated. In addition to the measurement of the glycated hemoglobin (HbA1c) concentration, the glucose concentration is measured by the measuring device 24A.

  In the detection device 70, the respective lights emitted from the LEDs 76 </ b> A to 76 </ b> D are condensed by the first optical system 72 through the condensing optical system 78 and guided to the reaction container 42 containing the sample solution L. Therefore, in the detection device 70, for example, the light emitted from the plurality of light sources is emitted from the LEDs 76A to 76D, respectively, as compared with the light reflected by the half mirrors and collected on the same optical path to be guided to the sample solution. It is possible to suppress a decrease in the amount of light when collecting the light.

  Further, the second optical system 74 disperses the light transmitted through the sample solution L, and the dispersed light is made into light having different wavelengths by the bandpass filters 95A to 95D and is guided to the respective light receiving elements 96A to 96D. These light receiving elements 96A to 96D measure (detect) the amount of received light. When the specific component in the sample solution L or the substance generated by being converted from the specific component absorbs the light of the specific wavelength, the amount of light measured by the light receiving element 96 that receives the light of the specific wavelength. Is decreased, the concentration of the specific component (eg, glycated hemoglobin (HbA1c)) contained in the sample can be calculated.

  Further, in the detection device 70, since the LEDs 76A to 76D are arranged in a matrix on the substrate 77, for example, as compared with one in which a plurality of light sources are arranged in a line, a collection device that receives and collects emitted light. The size (outer diameter) of the optical lens 80A can be reduced. As a result, the size of the housing 65A can be reduced.

  In the analyzer 10, the reaction container 42 is configured to pass on the optical path OL1 from the first optical system 72 to the second optical system 74. As a result, when the reaction table 38 is rotated, the plurality of reaction vessels 42 sequentially pass on the optical path OL1, so that, for example, the reaction vessel containing the sample solution is moved on the optical path from the first optical system to the second optical system each time. As compared with the configuration in which the sample solution L is transported to, the light condensed by the first optical system 72 can be efficiently applied to the sample solution L contained in the plurality of reaction vessels 42. This makes it possible to efficiently calculate the concentration of the specific component contained in the sample.

  Further, in the analyzer 10, the optical path OL2 of the light condensed by the condensing optical system 78 is extended above the base plate 39, and is made to face the base plate 39 in the horizontal direction by the reflection mirror 84. For example, the width (length along the horizontal direction) of the analyzer 10 can be narrowed as compared with the case where the optical path of the light condensed by the condensing optical system is extended horizontally with respect to the base plate 39.

  In the above-described embodiment, the light emitting diodes 76A to 76D are arranged in a matrix on one substrate 77, but the present invention is not limited to this structure. For example, the light emitting diodes 76A to 76D may be arranged in a line on a single substrate 77.

  Further, in the above-described embodiment, the LED light source unit 65 including the first optical system 72 is arranged below the reaction table 38 and on the rotary shaft 38A side, and the light receiving unit 66 including the second optical system 74 is arranged in the reaction table 38. Although the configuration is arranged in the periphery, the present invention is not limited to this configuration, and the arrangement positions of the LED light source unit 65 and the light receiving unit 66 may be reversed.

  Although one embodiment of the present invention has been described above, the present invention is not limited to the above, and various modifications other than the above can be carried out without departing from the spirit of the invention. Of course.

10 Analyzer 38 Reaction Table 38A Rotating Shaft 39 Base Plate 42 Reaction Vessel (Container)
70 Detection Device 72 First Optical System 74 Second Optical System 76 Light Emitting Diode 77 Substrate 80 Condensing Lens 84 Reflecting Mirror 96 Light Receiving Element L Specimen Solution (Sample Solution)
OL1 optical path OL2 optical path

Claims (5)

Four light-emitting diodes that emit light of different wavelengths,
The light emitted from each of the four light-emitting diodes is condensed through one condenser lens or a plurality of condenser lenses arranged in parallel on one optical axis, and the sample solution is contained in the light transmission A first optical system leading to a container having
The light transmitted through the container is divided into the same number as the number of the light emitting diodes, and the divided light is led to four light receiving elements of the same number as the number of the light emitting diodes as light having different wavelengths through a bandpass filter. A second optical system,
Equipped with
The second optical system includes a first reflection mirror that reflects the light transmitted through the container downward, and a first half that horizontally reflects the light reflected downward by the first reflection mirror and transmits the light downward. A mirror, a second reflecting mirror that reflects the light transmitted through the first half mirror in the horizontal direction, and a second half mirror that transmits the light reflected by the first half mirror in the horizontal direction and reflects the light downward. A third reflection mirror that horizontally reflects the light reflected by the second half mirror, and a third half mirror that transmits the light reflected by the second reflection mirror in the horizontal direction and reflects the light downward. A fourth reflecting mirror that horizontally reflects the light reflected by the third half mirror,
The four light receiving elements receive a first light receiving element that receives the light transmitted through the second half mirror through a first band pass filter and a light that is reflected by the third reflecting mirror through a second band pass filter. A second light receiving element, a third light receiving element for receiving the light transmitted through the third half mirror through a third bandpass filter, and a third light receiving element for receiving the light reflected by the fourth reflecting mirror through a fourth bandpass filter 4 light-receiving elements ; The first optical system is a collection formed by arranging two or more optical systems in which one condensing lens and one plano-concave lens for collimating the light condensed by the condensing lens as one set are arranged vertically. The detection device according to claim 1, further comprising an optical optical system. The detection device according to claim 1 , wherein the four light emitting diodes are arranged in a matrix on a substrate. Base plate,
A disc-shaped reaction table provided above the base plate and rotatable with respect to the base plate about a rotation axis extending in the vertical direction;
A plurality of containers, which are provided in the outer peripheral portion of the reaction table at intervals in the circumferential direction of the reaction table and have the light transmissive property, in which the sample solution is stored,
A detection device according to any one of claims 1 to 3 installed on the base plate,
Equipped with
The first optical system of the detection device is arranged on the rotation shaft side of the container of the reaction table,
The second optical system of the detection device is arranged in a peripheral portion of the reaction table,
An analyzer in which the container passes on an optical path from the first optical system to the second optical system. The first optical system is an optical system in which an optical path of light condensed through one or a plurality of the condensing lenses extends upward of the base plate, and is directed in a horizontal direction with respect to the base plate by a reflection mirror. The analyzer according to claim 4 .

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