JP6674817B2 - Wastewater treatment method and wastewater treatment kit - Google Patents

Description

  The present invention relates to a wastewater treatment method and a wastewater treatment kit.

  Wastewater from kitchens and food factories usually contains garbage and cooking oil. Solid matter such as garbage can be easily removed from the wastewater by providing a basket or the like at the drain, but it is not easy to remove liquid matter such as cooking oil. Therefore, in a facility such as a kitchen or a food factory that discharges wastewater mixed with a large amount of oil, a grease strap for accumulating the oil and separating and discarding the oil floating on the upper layer is provided.

  However, the oil accumulated in the grease trap may be solidified and remain on the water surface of the grease trap as scum (a lump of oil). At this time, the accumulated oil is oxidized and decomposed, which may cause odor and pests. Also, if the accumulated oil is left untreated, the oil removal capacity of the grease trap decreases, and the oil leaks to sewage or rivers. Therefore, when oil accumulates in the grease trap, it is necessary to request a specialized contractor to remove the oil by vacuum processing, high-pressure cleaning processing, or the like, which increases costs.

  Therefore, various methods for efficiently reducing oil content of microorganisms using microorganisms have been studied. For example, Patent Literature 1 discloses a method in which a lipase-secreting microorganism is added into a grease trap to decompose fats and oils into glycerol and fatty acids. However, when using microorganisms, it is necessary to perform aeration and stirring in order to grow the microorganisms.

  As a method that does not require stirring, Patent Literature 2 discloses a method in which microorganisms (yeast) having fat / oil decomposability are supported on a sponge as a porous carrier to decompose fat / oil in wastewater.

JP 2010-227849 A International Publication No. 2008/075678

  However, the method described in Patent Literature 2 has a problem that the oil decomposition rate by microorganisms is not sufficient.

  Therefore, the present invention has been made in view of the above circumstances, and an object of the present invention is to provide a method for improving the oil decomposition rate of oil-decomposing bacteria in a grease trap.

  The present inventors have intensively studied to solve the above problems. As a result, the present inventors have found that the above problem can be solved by a wastewater treatment method, which comprises contacting wastewater with an oil-decomposition improving material formed by coating an inorganic powder with a silicone compound, and an oil-decomposing bacterium. The invention has been completed.

  ADVANTAGE OF THE INVENTION According to this invention, the method of improving the oil-decomposition rate by an oil-decomposing bacterium in a grease trap is provided.

FIG. 1 schematically shows a mechanism of wastewater treatment using a grease trap.

  Hereinafter, embodiments of the present invention will be described. Note that the present invention is not limited to only the following embodiments. In addition, the dimensional ratios in the drawings are exaggerated for convenience of description, and may be different from the actual ratios.

  In this specification, “X to Y” indicating a range means “X or more and Y or less”, “weight” and “mass”, “weight%” and “mass%”, and “weight part” and “weight part”. Parts by mass "are treated as synonyms. Unless otherwise specified, the operation and measurement of physical properties are performed under the conditions of room temperature (20 to 25 ° C.) / Relative humidity of 40 to 50%.

<Wastewater treatment method>
According to one embodiment of the present invention, an oil-resolution improving material (hereinafter also referred to simply as “oil-resolution improving material”) in which inorganic powder is coated with a silicone compound, and an oil-decomposing bacterium are drained. A method for treating wastewater, comprising contacting is provided.

  According to the method for treating wastewater according to the present invention, it is possible to improve the oil decomposition rate by the oil decomposition bacteria in the grease trap. The estimated mechanism of the present invention will be described with reference to FIG. FIG. 1 schematically illustrates a mechanism of wastewater treatment by a grease trap 10.

  In the method according to the present invention, the oil-resolution improving material and the oil-decomposing bacteria may be added to the wastewater before being discharged to the grease trap 10, but typically, added to the wastewater in the wastewater treatment tank 1. Is done. However, the method according to the present invention is not particularly limited as long as the oil-resolution improving material and the oil-decomposing bacteria can be brought into contact with the wastewater.

  The installation form of the grease strap 10 is not particularly limited, such as an embedded type or a movable type. In the case of the buried type, for example, in a kitchen or a food processing plant, the grease strap 10 is buried so that the wastewater flowing out to the drainage channel is poured into the residue receiver 3. In the case of the movable type, for example, the grease strap 10 is installed so that the residue receiver 3 is located below the drainage groove of the sink.

  In FIG. 1, the drainage flows in the direction of the arrow. In addition, the charging of the drainage into the grease trap 10 may be a batch type or a continuous type. The oil-containing wastewater flows into the wastewater treatment tank 1 through the residue receiver 3. At this time, all or a part of the residue such as garbage is collected by the residue receiver 3, but most of the oil passes through the residue receiver 3 and flows into the wastewater treatment tank 1. The oil 6 flowing into the wastewater treatment tank 1 rises toward the water surface 5 and collects in a space partitioned by the partition plates 2a and 2c. Therefore, when the oil-resolution improving material and the oil-decomposing bacteria are not added to the wastewater, the oil 6 gradually aggregates in the space partitioned by the partition plates 2a and 2c to form scum.

  When the oil-resolution improving material and the oil-degrading bacterium according to the present invention are applied to the grease trap 10, the oil-resolution improving material is used in the wastewater treatment tank 1 (mainly in a space partitioned by the partition plates 2a and 2c). And the oil-decomposing bacteria come into contact with the oil 6 in the waste water. The oil component 6 floats toward the water surface 5 and gathers in a space partitioned by the partition plates 2a and 2c to form an oil layer. However, the oil component resolution improving material can be dispersed in the oil layer. In addition, since the inorganic powder is coated with the silicone compound, the surface of the inorganic powder has lipophilicity (water repellency). Therefore, aggregation of the oil component in the waste water can be suppressed, and the viscosity of the oil component can be reduced. For this reason, in the space partitioned by the partition plates 2a and 2c, the contact area between the oil-decomposing bacteria and the oil component 6 increases, and the reaction between the oil-decomposing bacteria and the oil component 6 (that is, the oil decomposition rate) can be improved. it can. Therefore, the oil component 6 can be efficiently decomposed. In addition, the oil-decomposing bacteria are adsorbed to the oil layer formed by the oil component 6 whose viscosity has been reduced, and therefore can remain in the oil layer without settling at the bottom of the wastewater treatment layer 1. Therefore, the oil decomposition rate by the oil decomposition bacteria can be further improved.

  The above mechanism is only an estimation and does not limit the technical scope of the present invention.

[Oil resolution improving material]
The oil content resolution improving material according to the present invention is obtained by coating an inorganic powder with a silicone compound.

  The average particle size (in terms of volume) of the oil content resolution improving material is typically 0.1 to 100 μm, preferably 1 to 50 μm, more preferably 1 to 20 μm, and further preferably 5 μm. It is super less than 20 μm. When the average particle diameter of the oil content resolution improving material is within the above range, the material exhibits high dispersibility in the oil layer formed by the oil component in the drainage water, so that the effect of the present invention can be further enhanced. The average particle size of the oil content resolution improving material can be measured by a particle size distribution meter such as a laser diffraction type particle size distribution measuring device.

(Inorganic powder)
The inorganic powder according to the present invention is not particularly limited, and examples thereof include silica (including silica gel, white carbon, aerosil, and amorphous silica), mica, talc, sericite, kaolin, clay, bentonite, activated carbon, and carbon. Black etc .; titanium oxide (anatase type, rutile type), zinc oxide, magnesium oxide, ferrous oxide, ferric oxide, aluminum oxide (alumina), chromium oxide, cobaltous oxide, cobalt trioxide, cobalt oxide Dicobalt, nickel oxide, nickel oxide, tungsten oxide, thorium oxide, molybdenum oxide, manganese dioxide, manganese trioxide, uranium oxide, thorium oxide, barium oxide, yttrium oxide, zirconium oxide, cuprous oxide, oxidation Cupric, stannous oxide, stannic oxide, lead monoxide, lead tetroxide, Oxides such as lead oxide, antimony trioxide, antimony pentoxide, niobium oxide, ruthenium oxide, barium titanate, silver oxide, germanium oxide; aluminum hydroxide, magnesium hydroxide, zirconium hydroxide, titanium hydroxide, chromium hydroxide Hydroxides such as aluminum chloride, titanium chloride, zirconium chloride, calcium fluoride; halides such as barium sulfate, magnesium sulfate, calcium sulfate, aluminum sulfate, titanium sulfate, strontium sulfate, zinc sulfide, cadmium sulfide, antimony sulfide; Sulfates and sulfides such as calcium sulfide, silver sulfide, germanium sulfide, cobalt sulfide, tin sulfide, lead sulfide, nickel sulfide, manganese sulfide, and zinc sulfide; phosphates such as calcium phosphate, hydroxyapatite, and aluminum phosphate; silicon nitride Nitride such as boron nitride, magnesium nitride, titanium nitride, aluminum nitride, iron nitride, vanadium nitride, zirconium nitride, tantalum nitride; molybdenum silicide, barium silicate, magnesium silicate, strontium silicate, aluminum silicate, zeolite, etc. Silicon compounds or silicates of the following; carbonates such as calcium carbonate and magnesium carbonate; silicon carbide, titanium carbide, tantalum carbide, zirconium carbide, tungsten carbide, molybdenum carbide, hafnium carbide, chromium, vanadium carbide, boron carbide, uranium carbide , Beryllium carbide, etc .; gold, silver, palladium, rhodium, iridium, rhenium, ruthenium, osmium, etc .; nickel, copper, zinc, tin, cobalt, iron, aluminum, molybdenum, manganese, tungsten, gallium , Indium, technetium, titanium, zirconium, cerium, tantalum, niobium, hafnium, etc .; aluminum-magnesium alloy, iron-carbon alloy, iron-copper alloy, iron-nickel-chromium alloy, silver-gold alloy, palladium-gold alloy , Silver-palladium alloy, copper-nickel alloy, nickel-cobalt alloy, nickel-magnesium alloy, tin-lead alloy and the like. These inorganic powders can be used alone or in combination of two or more.

  In a preferred embodiment of the present invention, the inorganic powder is silica, more preferably silica gel, from the viewpoint of affinity with the silicone compound.

  The average particle size of the inorganic powder is typically 0.1 to 100 μm, preferably 1 to 50 μm, and more preferably 1 to 25 μm. The average particle size means a volume-based average particle size. The average particle size of the inorganic powder can be measured with a particle size distribution meter such as a laser diffraction type particle size distribution measuring device.

From the viewpoint of increasing the contact area with the oil component, the inorganic powder is preferably a porous particle or a porous material. If Although inorganic powder is porous particles or porous, the specific surface area of the inorganic powder is preferably from 5~2000m ² / g, more preferably 10~800m ² / g. Further, the pore volume of the inorganic powder is preferably from 0.01 to 5.0 ml / g, and more preferably from 0.01 to 2.0 ml / g. The specific surface area can be measured by the BET method, and the pore volume can be measured by the mercury intrusion method.

  The shape of the inorganic powder is not particularly limited, and may be spherical, true spherical, elliptical spherical, irregular, crushed, cylindrical, pellet, square, needle, columnar, crushed, scale-like, leaf-like, or flake. Shape, plate shape, confetti, polygonal shape, etc.

(Silicone compound)
The silicone compound according to the present invention is not particularly limited as long as it can impart lipophilicity (water repellency) to the inorganic powder by coating the inorganic powder. In a preferred embodiment of the present invention, from the viewpoint of imparting lipophilicity to the inorganic powder, the silicone compound is selected from methyl hydrogen silicone oil, alkoxy-modified silicone oil, amino-modified silicone oil, epoxy-modified silicone oil, and polyether-modified silicone oil. And at least one selected from the group consisting of carboxyl-modified silicone oils. Specific examples of the silicone oil include triethoxycaprylylsilane, methyl hydrogen polysiloxane, dimethyl polysiloxane / methyl hydrogen polysiloxane copolymer, triethoxysilylethyl polydimethylsiloxyethyl dimethicone, and triethoxysilylethyl polydimethylsiloxy. Ethylhexyl dimethicone, acrylic silicone resin and the like can be mentioned.

  The method of coating the inorganic powder with a silicone compound is not particularly limited, a conventionally known method, a method of dispersing inorganic powder particles in a solvent such as isopropyl alcohol, and then adding a silicone compound, or a dry method. A method of mixing with a mixer or the like can be used. For example, the methods described in JP-A-2003-183027 and WO2007 / 077673 can be applied in the same manner or with appropriate modifications.

  As the oil-resolution improving material used in the method according to the present invention, a commercially available product can be used. For example, Sunsphere (registered trademark) H-51-ET, H-121-ET (manufactured by AGC S-I-Tech Co., Ltd.) Etc. can be used.

  In the method according to the present invention, one of the above-mentioned oil content resolution improving materials can be used alone, or two or more can be used in combination.

[Oil decomposing bacteria]
The oil-decomposing bacteria used in the method according to the present invention is not particularly limited as long as it can decompose oil. Examples of the oil-decomposing bacteria include bacteria, filamentous fungi, and yeast.

  Examples of bacteria include the genus Bacillus, the genus Lactobacillus, the genus Rhodococcus, the genus Enterococcus, the genus Sphingomonas, and the genus Burkholdosa monas. The genus, Staphylococcus, Rhizobium, Acinetobacter, Alcaligenes, Serratia, Tetrasphatrostronas, Tetrasphaeranos moss Etc.

  Examples of the filamentous fungi include the genus Aspergillus, the genus Penicillium, the genus Rhizopus, the genus Fusarium, and the genus Humicola.

  Examples of yeast include the genera Yarrowia, Candida, Pichia, Hansenula, Saccharomyces, Kluyveromyces, and Trichosporon. In a preferred embodiment of the present invention, the yeast is selected from the group consisting of the above-mentioned yeasts from the viewpoint of oil resolution.

  The yeasts used in the method according to the invention are more particularly Yarrowia lipolytica, Yarrowia sp., Candida famata, Candida bombicola candy candy. ), Candida cylindracea, Candida rugosa, Candida utilis, Candida intermedia, Candida intermediaa, Candida intermediaa, Candida intermediaa Candida Antarctica ( andida antarctica, Candida sp., Pichia farinosa, Pichia kluyveri, Pichia canadensis, Pichia francisa (Pichia haplophila), Pichia membrana efaciens (Pichia membranaefaciens), Pichia rhodananensis (Pichia rhodananensis), Hansenura anomala (Hansenura anuranu ran amu namba ran sam ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam nu ran sam han ran sam ran sam)) Kapusurata (Hansenura capsulata), Hansenula ciferrii (Hansenura ciferrii), Hansenula polymorpha (Hansenula polymorpha), Saccharomyces bayanus (Saccharomyces bayanus), Saccharomyces Fell Mentha tee (Saccharomyces fermentati), Saccharomyces Norubenshisu (Saccharomyces norbensis), Saccharomyces Obiformis (Saccharomyces oviformis), Kluyveromyces marxianus (Kluyveromyces marxianus), Kluyveromyces lactis (Kluyveromyces lactis) ), Trichosporon brassicae, Trichosporon capitatus, Trichosporon cutaneum, Trichosporon fermentor, Trichosporon fermentans, Trichosporon fermentans (Trichosporon pullulans), Trichosporon sp. ) Can be exemplified.

  Among these yeasts, it is preferable to use yeast of the genus Yarrowia from the viewpoint of high oil content resolution. Examples of yeasts belonging to the genus Yarrowia include Yarrowia lipolytica ATCC 48436, Yarrowia lipolytica NBRC1548, Yarrowia lipolytica LM02-011 (Accession No. NITE P-01813), Yarrowia lipolytica NBRC0746, and Yarrowia lipo y aoRipa yRipo yRipo yi Ripori B ), Yarrowia sp. Such as Yarrow-01 YH-01, etc., but Yarrowia lipolytica is more preferable, Yarrowia lipolytica LM02-011 (Accession No. NITE P-01813) is preferable.

  The above oil-decomposing bacteria can be obtained from culture collections such as ATCC, NBRC, and DSMZ. The above-mentioned oil-decomposing bacteria can be used alone or in combination of two or more if they can coexist.

  The method of culturing the oil-decomposing bacteria used in the method according to the present invention may be any method as long as the oil-decomposing bacteria can grow and proliferate. For example, the medium used for cultivation of the oil-decomposing bacteria used in the method according to the present invention may be any of a solid or liquid medium, and a carbon source that can be used by the oil-decomposing bacteria, an appropriate amount of a nitrogen source, As long as the medium contains inorganic salts and other nutrients, either a synthetic medium or a natural medium may be used. Usually, the medium contains a carbon source, a nitrogen source and a mineral.

  The carbon source that can be used in the cultivation of the oil-decomposing bacteria used in the method according to the present invention is not particularly limited as long as the strain used can be assimilated. Specifically, considering the assimilation of oil-decomposing bacteria, glucose, fructose, cellobiose, raffinose, xylose, maltose, galactose, sorbose, glucosamine, ribose, arabinose, rhamnose, sucrose, trehalose, α-methyl-D -Glucoside, salicin, melibiose, lactose, melezitose, inulin, erythritol, glucitol, mannitol, galactitol, N-acetyl-D-glucosamine, starch, starch hydrolyzate, molasses, molasses, sugars such as molasses, wheat, rice, etc. Examples include natural products, alcohols such as glycerol, methanol and ethanol, organic acids such as acetic acid, lactic acid, succinic acid, gluconic acid, pyruvic acid and citric acid, and hydrocarbons such as hexadecane. The carbon source is appropriately selected in consideration of assimilation by the oil-decomposing bacteria to be cultured. Further, one or more of the above carbon sources can be selected and used.

  Further, as a nitrogen source that can be used in the culture of the oil-decomposing bacteria of the present invention, meat extract, fish meat extract, peptone, polypeptone, yeast extract, malt extract, soybean hydrolyzate, soybean powder, casein, milk casein, casamino acid, Various amino acids such as glycine, glutamic acid, and aspartic acid; organic nitrogen sources such as corn steep liquor; hydrolysates of other animals, plants, and microorganisms; ammonium salts such as ammonia, ammonium nitrate, ammonium sulfate, and ammonium chloride; and nitrates such as sodium nitrate And nitrites such as sodium nitrite, and inorganic nitrogen sources such as urea. The nitrogen source is appropriately selected in consideration of assimilation by the oil-decomposing bacteria to be cultured. Further, one or more of the above-mentioned nitrogen sources can be selected and used.

  Inorganic substances that can be used in the present invention include phosphates, hydrochlorides, sulfates, acetates, carbonates, bicarbonates, and chlorides such as magnesium, manganese, calcium, sodium, potassium, copper, iron, and zinc. And the like. The above-mentioned inorganic substance is appropriately selected in consideration of assimilation by the oil-decomposing bacteria to be cultured. In addition, one or more of the above inorganic substances can be selected and used.

  To efficiently decompose and assimilate the oil component to the oil-decomposing bacteria of the present invention or to maintain the oil-decomposing and assimilating ability of the oil-decomposing bacteria, an oil component may be added to the medium.

  As used herein, the term "oil" refers to edible or industrial fats and oils rich in glycerides such as triglycerides, diglycerides and monoglycerides, and fatty acids. Examples of the oil added to the medium include olive oil, canola oil, coconut oil, sesame oil, rice oil, rice bran oil, safflower oil, soybean oil, corn oil, rapeseed oil, palm oil, palm kernel oil, sunflower oil, cottonseed oil Edible oils such as coconut oil, peanut oil, beef tallow, lard, chicken oil, fish oil, whale oil, butter, margarine, fat spread, shortening; linseed oil, jatropha oil, tall oil, hamana oil, castor oil, jojoba oil, etc. Industrial fats and oils; butyric, hexanoic, heptanoic, octanoic, decanoic, lauric, tridecanoic, myristic, pentadecanoic, pentadecanoic, palmitic, heptadecanoic, stearic, arachidic, behenic, Lignoceric acid, etc., decenoic acid, myristoleic acid, pentadecenoic acid, palmitolein , Heptadecenoic acid, oleic acid, icosenoic acid, docosenoic acid, tetracosenoic acid, hexadecadienic acid, hexadecatrienoic acid, hexadecatetraenoic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, octadecatetraenoic acid And fatty acids such as icosadienoic acid, icosatrienoic acid, icosatetraenoic acid, arachidonic acid, icosapentaenoic acid, henicosapentaenoic acid, docosadienoic acid, docosatetraenoic acid, docosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid.

  The amount of the oil added to the medium is not particularly limited, and may be appropriately selected in consideration of the ability of the oil-decomposing bacteria to be cultured to decompose and assimilate the oil. Specifically, it is preferable to add oil at a concentration of 1 to 30 g, more preferably 5 to 15 g, in 1 L of medium. With such an addition amount, the oil-decomposing bacteria can maintain a high oil-decomposition / assimilation ability. The oil may be added alone or in the form of a mixture of two or more.

  The cultivation of the oil-decomposing bacteria of the present invention can be performed by a usual method. For example, cultivation is performed under aerobic conditions or anaerobic conditions depending on the type of oil-decomposing bacteria. In the former case, the culture of the oil-decomposing bacteria is carried out by shaking or aeration and stirring. The oil-decomposing bacteria may be cultured continuously or in batches. Culture conditions are appropriately selected depending on the composition of the medium and the culture method, and are not particularly limited as long as the oil-degrading bacteria used in the method according to the present invention can grow, and are appropriately determined depending on the type of the oil-degrading bacteria to be cultured. Can be selected. Usually, the culture temperature is preferably 15 to 50 ° C, more preferably 25 to 40 ° C. The pH of the medium suitable for culture is preferably 3 to 11, more preferably 5 to 8. Further, the culture time is not particularly limited, and varies depending on the type of the oil-decomposing bacteria to be cultured, the amount of the medium, the culture conditions, and the like. Usually, the culture time is preferably 16 to 48 hours, more preferably 20 to 30 hours.

  The oil-decomposing bacteria used in the method according to the present invention may be variously suspended in a culture solution, recovered as a solid from the culture solution, dried, immobilized on a carrier, etc. Can be brought into contact with wastewater in any form. The oil-decomposing bacteria suspended in the culture solution, recovered as a solid content from the culture solution, or dried are added to, for example, wastewater and brought into contact with the wastewater. The oil-degrading bacteria immobilized on the carrier may be added to the wastewater, but the carrier on which the oil-degrading bacteria are immobilized is placed in a grease trap, and the oil is degraded by passing the wastewater through the carrier. Bacteria can be brought into contact with wastewater.

  When an oil-decomposing bacterium collected as a solid from a culture solution is used, any method known to those skilled in the art can be used as a collecting method. For example, the culture solution of the oil-decomposing bacteria cultured by the above-described method can be obtained by solid-liquid separation by centrifugation, filtration, or the like, and collecting the solid content. If this solid is dried (for example, freeze-dried), a dried oil-decomposing bacterium can be obtained.

  The oil-degrading bacterium of the present invention may be used in a state of being immobilized on a carrier. The carrier for immobilizing the oil-decomposing bacteria is not particularly limited as long as it can immobilize the oil-decomposing bacteria, and the carrier generally used for immobilizing microorganisms may be the same or appropriately. Used with qualification. For example, a method of entrapping and fixing in a gel substance such as alginic acid, polyvinyl alcohol, gellan gum, agarose, cellulose, dextran, and a method of adsorbing and fixing on a surface of glass, activated carbon, polystyrene, polyethylene, polypropylene, wood, silica gel, etc. Can be used.

  The method for immobilizing the oil-decomposing bacteria on the carrier is also not particularly limited, and a general method for immobilizing microorganisms may be used in the same manner or appropriately modified. For example, an immobilization method by pouring a culture of oil-decomposing bacteria into a carrier, a fixing method by pouring the carrier under reduced pressure using an aspirator, and pouring a culture of oil-degrading bacteria into a carrier, and A method of pouring a culture solution into a mixture of a sterilized medium and a carrier, culturing with shaking, and naturally drying the carrier taken out of the mixture is mentioned.

  In a preferred embodiment of the present invention, the oil-degrading bacteria are used in the form of a live bacterial preparation from the viewpoint of the viability of the oil-degrading bacteria during storage. As the oil-decomposing bacteria, the above-mentioned oil-decomposing bacteria can be used. The oil-degrading bacterium is preferably a yeast selected from the group consisting of the genus Yarrowia, the genus Candida, the genus Pichia, the genus Hansenula, the genus Saccharomyces, the genus Kluyveromyces, and the genus Trichosporone from the viewpoint of the oil-degrading ability. When the yeast is used as an oil-decomposing bacterium, the viable cell preparation contains an oil-decomposing bacterium-containing layer formed on a porous carrier, and the oil-decomposing bacterium-containing layer is 1 part by weight of the oil-decomposed bacterium as a dry cell. Based on the bacterium, 0.8 to less than 1.5 parts by weight of trehalose, 0.01 to 5 parts by weight of milk protein, 0.03 to 10 parts by weight of amino acid, and 0.02 to 10 parts by weight of It contains metal salts. Trehalose is contained in the oil-decomposing bacterium-containing layer in an amount of at least 0.8 part by weight based on 1 part by weight of the oil-decomposing bacterium as a dry cell body, thereby improving the survival rate of yeast during formulation. In addition, when the amount is less than 1.5 parts by weight, a decrease in the survival rate of the yeast during the storage period of the live bacterial preparation can be prevented. From the viewpoint of a further preferable balance between the survival rate during the formulation of the oil-decomposing bacteria and the survival rate during the storage period, the trehalose content of the oil-degrading bacteria-containing layer is determined to be 1 part by weight of the oil-degrading bacteria in terms of dry cells. The amount is preferably more than 0.9 parts by weight and less than 1.5 parts by weight, more preferably 1 part by weight or more and 1.4 parts by weight or less.

  Note that disaccharides other than trehalose, for example, disaccharides other than trehalose such as sucrose, lactose, and maltose may be used alone or in combination of two or more, and used in combination with trehalose. In this case, the content of disaccharides other than trehalose is, for example, 0.01 parts by mass or more and less than 0.8 parts by mass, preferably 0.1 parts by mass, based on 1 part by weight of the dry bacterium-free spore-free spores. Not less than 0.5 part by mass.

  The milk protein in the oil-decomposing bacteria-containing layer is not particularly limited, and conventionally known materials containing various milk proteins can be used. Raw materials with a high milk fat content such as whole fat powdered milk can be used, but when producing a live-oil-fat-decomposing bacterial preparation such as used in a grease trap, it is preferable that the oil content in the preparation is small, so for example, Preferred examples include casein, sodium caseinate, whey, concentrated whey, whey protein concentrate (WPC), whey protein isolate (WPI), whey powder, skim milk, milk protein concentrate (MPC), skim milk powder, α -Lactalbumin, β-lactoglobulin and the like can be exemplified, and these can be used alone or in combination of two or more. The milk protein is contained in the oil-decomposing bacterium-containing layer in an amount of 0.01 to 5 parts by weight, preferably 0.02 to 1 part by weight, more preferably 0 to 1 part by weight per 1 part by weight of the oil-decomposing bacteria as dry cells. By containing 0.05 to 0.5 parts by weight, the survival rate of the oil-decomposing bacteria at the time of formulation can be improved.

  Amino acids in the oil-degrading bacteria-containing layer include, for example, glycine, alanine, valine, leucine, isoleucine, phenylalanine, glutamine, glutamic acid, asparagine, aspartic acid, cysteine, lysine, serine, threonine, methionine, tryptophan, histidine, arginine, proline, Examples include tyrosine and derivatives thereof. Examples of amino acid derivatives include salts (eg, potassium salts and sodium salts), esters, hydrates, and solvates of amino acids. The above amino acids can be used alone or in combination of two or more. The amino acid is contained in the oil-decomposing bacterium-containing layer in an amount of 0.03 to 10 parts by weight, preferably 0.1 to 5 parts by weight, more preferably 0.1 to 5 parts by weight, based on 1 part by weight of the dried oil-decomposing bacteria. By containing 1 to 3 parts by weight, the survival rate of the oil-decomposing bacteria at the time of formulation can be improved. When two or more amino acids are contained in the oil decomposition layer, the above numerical values indicate the total amount of two or more amino acids.

  Metal salts in the oil-decomposing bacteria-containing layer include, for example, sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), nickel (Ni), and zinc (Zn). ), Sulfates, sulfites, hyposulfites, persulfates, thiosulfates, carbonates, phosphates, pyrophosphate, hydrochlorides, nitrates, nitrites, acetates of metal elements such as copper (Cu) , Propionate, butyrate, citrate, oxalate, halides (eg, fluoride, chloride, bromide, iodide) and the like, but are not limited thereto. More specifically, metal salts include, for example, sodium sulfate, sodium sulfite, sodium hyposulfite, sodium thiosulfate, sodium carbonate, sodium persulfate, monosodium phosphate, disodium phosphate, trisodium phosphate, sodium acetate , Sodium nitrate, sodium nitrite, sodium acetate, sodium citrate, sodium oxalate, sodium chloride, potassium sulfate, potassium sulfite, potassium hyposulfite, potassium thiosulfate, potassium carbonate, potassium persulfate, monopotassium phosphate, phosphoric acid Dipotassium, tripotassium phosphate, potassium acetate, potassium nitrate, potassium nitrite, potassium acetate, potassium citrate, potassium oxalate, potassium chloride, magnesium sulfate, magnesium sulfite, magnesium thiosulfate, magnesium carbonate, primary lithium Magnesium oxide, dibasic magnesium phosphate, tertiary magnesium phosphate, magnesium pyrophosphate, magnesium nitrate, magnesium nitrite, magnesium acetate, magnesium citrate, magnesium oxalate, magnesium chloride, calcium sulfate, calcium sulfite, calcium thiosulfate, Examples thereof include calcium carbonate, calcium nitrate, calcium nitrite, calcium acetate, calcium citrate, calcium oxalate, and calcium chloride. The above metal salts can be used alone or in combination of two or more. The metal salt is contained in the oil-decomposing bacteria-containing layer, and as dry cells, 1 part by weight of the oil-decomposing bacteria, 0.02 to 10 parts by weight, preferably 0.05 to 1.5 parts by weight, more preferably By containing 0.1 to 1 part by weight, it is possible to prevent a decrease in the survival rate of the oil-decomposing bacteria during the storage period of the live bacterial preparation. When the oil-decomposing bacteria-containing layer contains two or more metal salts, the above numerical values indicate the total amount of two or more metal salts.

  From the viewpoint that the survival rate of the oil-degrading bacteria during the storage period is particularly excellent, the metal salt is an element selected from the group consisting of Na, K, Mg, and Ca, sulfate, carbonate, phosphate, or a salt thereof. Is preferable, and a sulfate of Mg and / or Ca is more preferable. The above “metal salt” includes hydrates and solvates of the metal salt.

  In the oil-decomposing bacteria-containing layer, in addition to the above-mentioned components, optionally, monosaccharides such as glucose and fructose; disaccharides other than trehalose such as sucrose, lactose and maltose; cyclodextrin, hydroxypropylcellulose, and hydroxypropylmethylcellulose , Carboxymethylcellulose, crystalline cellulose, polysaccharides such as corn starch; proteins such as soybean proteins; protein hydrolysates and peptides such as soybean peptides, gelatin, peptone, and tryptone; oils and fats such as soybean oil, rapeseed oil, palm oil, sesame oil, and olive oil ; Vitamins such as ascorbic acid and its salts, tocopherol; surfactants such as polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester; polyethylene glycol, glycerin, etc. It may be.

  The material of the porous carrier that can be used for the viable bacterial preparation is not particularly limited, but examples thereof include diatomaceous earth, perlite, vermiculite, talc, clay, zeolite, bentonite, kaolin, calcium carbonate, activated clay, and titanium dioxide. , Silica sand, pumice, activated carbon, carbon black, graphite, polylactic acid, polystyrene, polyvinyl chloride, and the like, but are not limited thereto. The material of the porous carrier is preferably diatomaceous earth from the viewpoint of the survival rate of the oil-decomposing bacteria at the time of formulation.

  The term “porous” in a porous carrier that can be used in the above-mentioned viable cell preparation refers to a state in which the surface of the carrier has a large number of small cell-like voids. Preferably, the bulk density (tapping bulk density) of the porous carrier is 0.01 to 2 g / ml. By using the porous carrier having the above bulk density, the survival rate of the oil-decomposing bacteria after the formulation step can be further improved. The bulk density of the porous carrier is more preferably 0.05 to 1.5 g / ml, and still more preferably 0.2 to 1 g / ml. The above bulk density is a value measured by a tapping bulk density measurement method.

  From the viewpoint of the survival rate of the oil-decomposing bacteria during formulation, the carrier is preferably in the form of particles having an average particle size (diameter, volume basis) of 1 to 300 μm, and the average particle size (diameter, volume basis). Is more preferably from 10 to 200 μm, more preferably from 30 to 150 μm. The particle size of the carrier is a value measured by a laser diffraction method.

  The viable bacterial preparation can be formulated by spraying a spray liquid onto a porous carrier that can be used in the viable bacterial preparation. The spray liquid can be prepared by adding the above oil-decomposing bacteria, trehalose, milk protein, amino acids, metal salts and other components as necessary to water, and stirring as necessary. As the water used for preparing the spray liquid, for example, tap water, distilled water, ion-exchanged water, pure water, ultrapure water, and the like may be used, but distilled water, ion-exchanged water, and pure water with little impurities may be used. Or ultrapure water is preferably used. A mixed solution in which a lower alcohol such as methanol, ethanol or propanol, or a polar solvent such as acetone is appropriately added to water may be used.

  Spraying of the spray liquid onto the porous carrier that can be used for the above-mentioned viable cell preparation can be performed using, for example, a fluidized bed granulator. By formulating by a fluidized bed granulation method, an oil-degrading bacteria-containing layer can be formed on a porous carrier at a relatively low temperature. For this reason, it is advantageous from the viewpoint of improving the survival rate at the time of formulation. In addition, by preparing a preparation by a fluidized-bed granulation method, the oil-degrading bacteria-containing layer can be advantageously formed uniformly on a porous carrier.

  The spray amount of the spray liquid on the porous carrier can be arbitrarily set, and is not particularly limited. However, for 1 part by weight of the porous carrier, the solid content of the spray liquid is, for example, 0.1 to 10% by weight. Parts, preferably 0.5 to 2 parts by weight.

  The spray liquid is usually sprayed onto the porous carrier at a rate of about 50 to 300 g / min.

  When the preparation is made by a fluidized-bed granulation method, the temperature of the hot air may be appropriately set in consideration of the heat resistance of the oil-decomposing bacteria, for example, the inlet temperature is 30 to 60 ° C, and preferably 35 to 60 ° C. 50 ° C. The temperature of the fluidized bed may be appropriately set in consideration of the heat resistance of the oil-decomposing bacteria, and is, for example, 30 to 60 ° C, and preferably 35 to 50 ° C. After the spraying, the porous carrier sprayed with the sprayed liquid may be dried as it is in a fluidized bed granulator, or may be dried separately by a dryer.

  The powdered live bacterial preparation may be partially or entirely coated with a known coating agent depending on the use. In addition, powders such as lubricants (eg, talc, mica, silica, magnesium stearate), desiccants (calcium oxide, silica gel), fillers, and the like may be mixed at an arbitrary ratio with a viable bacterial preparation to form a mixed preparation. . Further, the preparation may be pulverized or classified so as to have a desired particle size.

  From the viewpoint of the survival rate of the oil-decomposing bacteria during the storage period, the water content of the live bacterial preparation after formulation is preferably 3 to 8% by weight, and more preferably 4.5 to 7.5% by weight. More preferred.

[Processing method]
In the method according to the present invention, the amount of the oil-resolution improving material added to the wastewater can be arbitrarily set. The amount of the oil content resolution improving material added to the wastewater is not particularly limited, but is 0.001 to 5 g, preferably 0.01 to 0.5 g, per 1 g of the oil content contained in the wastewater. Alternatively, the amount is, for example, 0.001 to 5% (w / v), preferably 0.01 to 0.5% (w / v) with respect to the drainage in the grease trap. By adding 0.001% (w / v) or more to the wastewater, the oil content can be efficiently reduced. By setting the addition amount of the oil-resolution improving material to 5% (w / v) or less, it is possible to prevent the added oil-resolution improving material from excessively flowing out to the external environment.

In the method according to the present invention, the amount of bacteria to be added can be arbitrarily set. The amount of bacteria to be added to the wastewater is not particularly limited, but is 1 × 10 ⁴ to 1 × 10 ¹² CFU, preferably 1 × 10 ⁵ to 1 × 10 ^11, for 1 g of oil contained in the waste water. CFU. Alternatively, the amount may be, for example, 1 × 10 ⁶ to 1 × 10 ¹² CFU / L, more preferably 1 × 10 ⁷ to 1 × 10 ¹¹ CFU / L, with respect to the drainage in the grease trap. . When two or more types of oil-decomposing bacteria are used in combination, the total amount means the total amount. The oil-decomposing bacteria added to the wastewater may be pre-cultured. By pre-culturing, the amount of bacteria to be inoculated can be easily adjusted. Also, the amount of bacteria to be inoculated can be easily adjusted by using an oil-decomposing bacterium in the form of a live bacterial preparation.

  In discharging the wastewater to the external environment, the oil-decomposing bacteria are discharged to the outside of the grease trap together with the wastewater. Therefore, in the present invention, it is preferable to periodically add the oil-decomposing bacteria to the grease trap (drainage). The interval is not particularly limited, but, for example, it is preferable to add once every 3 hours, once every 16 hours, once every 24 hours, or once every 2 to 3 days. There is no particular limitation on the method of addition, and when the wastewater continuously flows into the grease trap, the wastewater may be mixed with the wastewater and added, or may be directly added to the wastewater in the grease trap. If oil-decomposing bacteria are added from a drain port of a kitchen sink or the like, the oil-decomposing bacteria can be introduced into the grease trap together with the drainage discharged by washing.

  The oil content resolution improving material in the wastewater may be added to the wastewater simultaneously with the oil-decomposing bacteria, or may be added separately. Preferably, the oil resolving agent is added to the wastewater once every one to twelve weeks, preferably once every two to eight weeks.

  Examples of the oil contained in the wastewater include the above-mentioned edible fats and oils, industrial fats and oils, and fatty acids. The oil content in the wastewater is not particularly limited. The oil content in the wastewater may be two or more.

  In the method according to the present invention, other components may be added to the wastewater in addition to the oil-resolution improving material and the oil-decomposing bacteria. Other components include, for example, lipase, pH adjuster and the like.

  In the method according to the present invention, a lipase may be added to the wastewater to assist the oil-decomposing bacteria in decomposing the oil. The lipase, for example, Pseudomonas (Pseudomonas), especially Pseudomonas cepacia (Pseudomonas cepacia), Pseudomonas stutzeri (Pseudomonas stutzeri), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Pseudomonas Alcaligenes (Pseudomonas alcaligenes), Pseudomonas fluorescens (Pseudomonas fluorescens), Pseudomonas flagi, Pseudomonas maltophilia, Pseudomonas mendocina a), Pseudomonas Mefichika lipolytica (Pseudomonas mephitica lipolytica), Pseudomonas Alcaligenes (Pseudomonas alcaligenes), Pseudomonas Purantarii (Pseudomonas plantari), Pseudomonas shoe de alkali monocytogenes (Pseudomonas pseudoalcaligenes), Pseudomonas putida (Pseudomonas putida) and Pseudomonas Wiesbaden Strains of Pseudomonas wisconsinensis, Aspergillus, especially Aspergillus niger and Aspergillus niger Strains of Aspergillus flavus, Bacillus, especially Bacillus stearothermophilus, Bacillus pucilus, Bacillus licilnibacillus bacillus bacillus bacillus bacillus bacillus bacillus bacillus bacillus bacillus Bilis flavus, strains of Aspergillus flavus, Bacillus, in particular Bacillus stearothermophilus, Bacillus pucilus, and Bacillus licheniformis (Bacillus bilici sulciformis). The strains of Penicillium, especially Penicillium cyclopium, Penicillium crustosum and Penicillium expansum, Rhizopus izopus), in particular Rhizopus japonicus (Rhizopus japonicus), Rhizopus oryzae (Rhizopus oryzae), Rhizopus delemar (Rhizopus delemar), a strain of Rhizopus Mikurosuporusu (Rhizopus microsporus) and Rhizopus Nodosasu (Rhizopus nodosus), Rhizomucor (Rhizomucor ), Especially strains of Rhizomucor miehei, strains of Mucor, strains of Paecilomyces, strains of Rhizoctonia (especially, strains of Rhizoctonia solias, Rhizonia abscis, Rhizonia absorpti) Strains of Absidiah blakesleena and Absidiah corimbifera, Achromobacter, especially Achromobacter iophaaus, Achromobacter iophagus, and Achromobacter iophaus aerophags. In particular, strains of Alternaria brassiciola, Aureobasidium, especially strains of Aureobasidium pullulans, strains of Beauveria cromobola, Beauvera cromobola cter), especially strains of Chromobacter viscosum, strains of Coprinus, especially Coprinus sinerius, Fusarium, especially Fusarium oxysporum, Fusarium oxysporum solani), strains of Fusarium solani pisi and Fusarium roseum culmorum, strains of Geotricum, especially strains of Geotricum penicillatum (Humikom ticum, Gumtric humicola) a), especially Humicola brevispora, Humicola brevis var. Himicola brevis var. strains of P. thermoidea and Humicola insolens; strains of Hyphozyma; ), Especially strains of Rhodosporidium toruloides, and / or Trichoderma, especially Trichoderma harzianum and Trichoderma reesei strains of Trichoderma reesei from Trichoderma reesei strains. Obtainable. Lipases obtained from yeasts of the genera Yarrowia, Candida, Pichia, Hansenula, Saccharomyces, Kluyveromyces, and / or Trichosporon can also be used.

  Commercially available lipases include lipase MY and lipase OF, lipase PL, lipase QLM (Meito Sangyo Co., Ltd.); lipase A “Amano (registered trademark)” 6, lipase M “Amano (registered trademark)” 10, lipase G “ Amano (registered trademark) 50, lipase F-AP15, lipase AY "Amano (registered trademark)" 30G, lipase R "Amano (registered trademark)" G and lipase T "Amano (registered trademark)" (Amano Enzyme Co., Ltd.); Sumiteam (registered trademark) NLS, Sumiteam (registered trademark) RLS (Shin Nippon Chemical Industry Co., Ltd.); and Lilipase (registered trademark) A-10D, Lilipase (registered trademark) AF-5 (Nagase ChemteX Corporation); Entilon AKG -2000, Entilon LP, Entilon LPG (Rakuto Kasei Kogyo Co., Ltd.); Lipo ase (R) 100T, Lipolase (TM) 100L, Platase20000L, Lipex (TM) 100T, Lipex (TM) 100L (manufactured by Novozymes) and the like.

  The amount of the lipase is not particularly limited as long as the lipase can react with the oil, but it is preferably used at 10 to 2,000 U per 1 g of the oil contained in the wastewater. More preferably, it is 50 to 1,500 U, and still more preferably 100 to 1,000 U. Alternatively, the amount may be preferably 1,000 to 100,000 U / L, more preferably 2,000 to 80,000 U / L, based on the capacity of the grease trap. The lipase activity unit (U) is the amount of enzyme that releases 1 μmol of fatty acid per minute under the conditions of 37 ° C. and pH 7.

  Examples of the pH adjuster include an acid and an alkali. When the pH of the wastewater is not within the range of the optimum pH of the oil-decomposing bacteria, it is preferable to adjust the pH to an optimum pH by adding an acid or an alkali. At this time, the acid or alkali is not particularly limited, but an acid such as hydrochloric acid, nitric acid, sulfuric acid, or acetic acid, or an alkali such as sodium hydroxide or potassium hydroxide is used. The content of the pH adjuster is not particularly limited, and may be an amount that achieves a desired pH.

  The grease trap may be a form that continuously introduces fats or oils or fatty acids containing wastewater and continuously discharges treated wastewater, or after introducing fats or oils or fatty acids containing wastewater and treats them collectively, The wastewater after the treatment may be collectively discharged.

  In the method of the present invention, the temperature at which the oil-decomposing bacteria decomposes the oil, that is, the temperature inside the grease trap, can be appropriately selected because it differs depending on the oil-decomposing bacteria used. Further, the pH at which the oil-decomposing bacteria decomposes the oil, that is, the pH in the grease trap, can be appropriately selected because it differs depending on the oil-decomposing bacteria used. For example, in general, the temperature is preferably from 10 to 60C, more preferably from 20 to 50C, and even more preferably from 25 to 40C. The pH is preferably 3 to 10, more preferably 4 to 9, and even more preferably 5 to 8. Furthermore, if necessary, aeration may be performed on the wastewater by aeration or the like within a range that satisfies the wastewater standard. However, in the treatment method according to the present invention, the oil content in the wastewater can be efficiently removed without aeration or the like. It is excellent in that it can be decomposed.

<Wastewater treatment kit>
According to another aspect of the present invention, there is provided a wastewater treatment kit including an oil component resolution improving material obtained by coating an inorganic powder with a silicone compound, and an oil degrading bacterium. Such a kit can improve the oil decomposition rate even if aeration is performed within a range that satisfies the drainage standards.However, the treatment kit according to the present invention efficiently reduces the oil content in the wastewater without aeration. Can be decomposed. In the kit according to the present invention, the oil-resolution improving material comprising an inorganic powder coated with a silicone compound, the oil-degrading bacterium, and optionally contained components (lipase, pH adjuster, etc.) are contained in the same container. However, they may be arbitrarily stored in another container. Regarding the oil-resolution improving material and the oil-decomposing bacterium in which the inorganic powder contained in the wastewater treatment kit according to the present invention is coated with a silicone compound, the oil-degrading material and the oil-decomposing bacterium in the above-described wastewater treatment method are used. Same as described.

  The oil-decomposing bacteria contained in the wastewater treatment kit according to the present invention is preferably in the form of a viable cell preparation from the viewpoint of the viability of the oil-decomposing bacteria during storage. The viable bacterial preparation contained in the wastewater treatment kit according to the present invention is the same as the contents described in the above wastewater treatment method.

  Further, the wastewater treatment kit according to the present invention may further include the above-mentioned lipase, pH adjuster, and the like in an arbitrary ratio as long as the effects of the present invention are not impaired.

  In the kit according to the present invention, the container containing the oil-resolution improving material, the oil-decomposing bacteria, and optionally contained components (lipase, pH adjuster, and the like) is not particularly limited. For example, brown glass), metal, ceramic, plastic, and the like can be used. The kit according to the present invention may arbitrarily further include an instruction manual describing the amount of parts used in the kit, the frequency of addition to the grease trap, and the like.

The wastewater treatment kit according to the present invention is used by adding at least the oil-resolution improving material and the oil-decomposing bacteria to the wastewater simultaneously or separately. The amount added to the wastewater is not particularly limited, and is appropriately set according to the amount of oil in the wastewater and the amount of the wastewater. For example, the amount of the oil-resolution improving material added to the wastewater is 0.001 to 5 g, preferably 0.01 to 0.5 g, per 1 g of the oil contained in the wastewater. Alternatively, the amount is, for example, 0.001 to 5% (w / v), preferably 0.01 to 0.5% (w / v) with respect to the drainage in the grease trap. The amount of bacteria added to the wastewater is, for example, 1 × 10 ⁴ to 1 × 10 ¹² CFU, preferably 1 × 10 ⁵ to 1 × 10 ¹¹ CFU, per 1 g of oil contained in the waste water. Alternatively, it is, for example, 1 × 10 ⁶ to 1 × 10 ¹² CFU / L, more preferably 1 × 10 ⁷ to 1 × 10 ¹¹ CFU / L, for the drainage in the grease trap.

  When the oil-decomposing bacteria are used in the form of a viable cell preparation, the amount of the viable cell preparation to be added to the wastewater is not particularly limited, and the amount of oil in the wastewater, the amount of the wastewater, and the amount of oil contained in the viable cell preparation It is appropriately set according to the number of viable decomposing bacteria. In the viable cell preparation according to the present invention, the amount of the viable cell preparation to be added to the wastewater is, for example, 0.0001 g to 300 g, and preferably 0.001 g to 30 g, based on 1 g of oil contained in the wastewater. Alternatively, the amount is, for example, 0.001 g to 300 g / L, more preferably 0.01 to 30 g / L, with respect to the drainage in the grease trap.

  The oil-resolution improving material contained in the wastewater treatment kit of the present invention is added to the grease trap (wastewater) at intervals of, for example, once every 1 to 12 weeks, preferably once every 2 to 8 weeks. The oil-decomposing bacteria contained in the wastewater treatment kit of the present invention may be, for example, grease traps once every 3 hours, once every 16 hours, once every 24 hours, or once every 2 to 3 days. (Drainage). Since the oil-degrading material and the oil-decomposing bacteria contained in the wastewater treatment kit have different preferable addition frequencies, they are preferably housed in separate containers.

  The effects of the present invention will be described using the following examples and comparative examples. However, the technical scope of the present invention is not limited only to the following examples.

[Preparation of test medium]
Each component was dissolved in distilled water so as to have the following composition, and the pH was adjusted to 5.0 with hydrochloric acid to prepare a test medium.

[Preparation of oil content]
Rapeseed oil (manufactured by Wako Pure Chemical Industries, Ltd.) and soybean oil (manufactured by Wako Pure Chemical Industries, Ltd.) were mixed at a ratio of 1: 1 (w / w) to prepare an oil component.

[Preparation of live bacterial preparation]
Yarrowia lipolytica LM02-011 strain (March 6, 2014, deposited at the National Institute of Technology and Evaluation, Patent Microorganisms Depositary Center (2-5-8 Kazusa Kamatari, Kisarazu-shi, Chiba) The accession number is NITE P-01813.) Was inoculated into the following liquid medium and cultured in a jar fermenter at 30 ° C. for 48 hours (stirring speed: 300 rpm) to obtain a culture solution. .

Preparation method of liquid medium: final concentration of 0.18% (w / v) polypeptone, 0.12% (w / v) meat extract, 0.07% (w / v) NaHCO ₃ , 0.005% (w) /v)NaCl,0.002%(w/v)KCl,0.002%(w/v)CaCl ₂ · _{2H 2} O, a 0.003% (w / v)) MgSO 4 · 7H 2 O As described above. After adjusting the pH to 5 with hydrochloric acid, autoclave sterilization was used as a liquid medium.

The obtained culture was centrifuged (× 10,000 g) for 10 minutes to collect the cells. The resulting 11 wt% of yeast as dry cells against bacteria, trehalose 14 weight%, the skim milk 3% (1% by weight as a milk protein), glycine 3 wt%, MgSO ₄ · 7H ₂ O 1 wt%, was added to distilled water to 1 mass% of CaSO ₄ · 2H ₂ O, was prepared by mixing the spray solution. As milk protein, skim milk containing 35% by weight of protein and 52% by weight of lactose in solid content was used.

  300 g of diatomaceous earth (average particle size: 75 μm, bulk density: 0.33 g / ml, Radiolite (registered trademark) $ 3000, Showa Chemical Industry Co., Ltd.) in a fluidized bed granulator (FA-LAB-1, Powrex Co., Ltd.) Was set. The diatomaceous earth was sprayed with 1077 g of the above spray liquid at a rate of 143 g / min, and dried while the contents were fluidized by blowing hot air at 40 ° C. After the spraying, subsequently, warm air at 40 ° C. was blown in the fluidized bed granulator for 40 minutes to dry the granulated product. Thus, a live bacterial preparation was obtained. The water content of the viable cell preparation was 5.5% by weight.

(Calculation of viable cell count in viable cell preparation)
The number of viable bacteria per 1 g of the viable cell preparation was calculated by the following method. In Examples and Comparative Examples, the viable cell count calculated before performing the Examples and Comparative Examples (within 48 hours) was used. The viable bacterial preparation was stored at 23 ° C. in a light-shielded glass vial.

  The viable bacterial preparation was serially diluted by stirring at 25 ° C. for 1 minute in 100-fold volume of distilled water, and the resulting diluted solution was added to an agar medium (composition: agar medium such that the final concentration was 20% by weight in the above liquid medium). Was added to the surface. After culturing the bacteria at 30 ° C. for 48 hours, the number of colonies formed on the agar medium was counted. From the number of colonies, the number of viable bacteria per 1 g of the preparation was calculated.

[Comparative Example 1]
The oil decomposition rate was determined by the following method.
(1) 200 mL of the test medium prepared above was dispensed into a 500 mL tall beaker.
(2) 1 g of the oil component prepared above was added to the tall beaker of (1).
(3) The tall beaker of (2) was autoclaved at 121 ° C. for 20 minutes and allowed to cool until the test medium and the oil content reached room temperature.
(4) 40 mg (oil-decomposing bacteria: 1.2 × 10 ⁸ CFU) of the prepared viable cell preparation was added to the tall beaker of (3).
(5) After adding the viable cell preparation, the tall beaker of (4) was allowed to stand in a constant temperature bath at 30 ° C. for 16 hours under static conditions.
(6) A 100 mL portion of the aqueous layer was taken from the tall beaker of (5), and 100 mL of a separately prepared new test medium (autoclaved at 121 ° C. for 20 minutes) was added to the tall beaker. The separated aqueous layer was discarded.
(7) After adding the test medium, the tall beaker of (6) was allowed to stand in a thermostat at 30 ° C. for 8 hours under static conditions.
(8) After performing (6) again, 1 g of oil and 40 mg of the above-prepared viable cell preparation (oil-decomposing bacteria: 1.2 × 10 ⁸ CFU) were added to the same tall beaker.
(9) After adding the oil component and the viable cell preparation, the tall beaker of (8) was allowed to stand in a constant temperature bath at 30 ° C. for 16 hours under static conditions.
(10) After standing, a normal hexane extract was prepared according to JIS K0102: 2013 (industrial wastewater test method). Using the normal hexane extract as the residual amount of the oil component, the oil decomposition ratio was determined from the total amount (2 g) of the added oil component and the residual amount of the oil component (the amount of the normal hexane extract (g)) by the following formula 1.

[Comparative Example 2]
Comparative Example 1 Same as (Comparative Example 1) except that in Example 4 (4) and (8), 10 mg of Shirasu balloon (Winlight WB601, manufactured by Axies Chemical Co., Ltd., average particle size: 180 μm) was further added and added to the tall beaker by 10 mg. To determine the oil decomposition rate.

[Example 1]
In (4) and (8) of Comparative Example 1, Sunsphere (registered trademark) H-51-ET (manufactured by AGC S-I-Tech Co., Ltd., average particle size: 5 μm; triethoxysilylethyl polydimethylsiloxyethylhexyl dimethicone) was added to the tall beaker. ) Was determined in the same manner as in Comparative Example 1, except that 10 mg of the oil-decomposed product was further added and added in 10 mg increments.

[Example 2]
Instead of Sunsphere (registered trademark) H-51-ET, Sunsphere (registered trademark) H-121-ET (manufactured by AGC S-ITEC, average particle size: 12 μm; silica gel coated with triethoxysilylethyl polydimethylsiloxyethylhexyl dimethicone) The oil decomposition ratio was determined in the same manner as in Example 1 except that the above-mentioned composition was used.

[Comparative Example 3]
Oil decomposition was carried out in the same manner as in Comparative Example 1 except that in (4) and (8) of Comparative Example 1, Sunsphere (registered trademark) H-121-ET was added and added in an amount of 10 mg each in place of the viable cell preparation. The rate was determined.

  Table 2 below shows the oil decomposition rates of Examples 1 and 2 and Comparative Examples 1 to 3.

  As is clear from the results shown in Table 2, the method of treating wastewater according to the present invention (Examples 1 and 2) shows that the oil-decomposing bacteria have an excellent oil-decomposition rate over Comparative Examples 1 and 2. I understand. Further, in Comparative Example 3 using only the oil-resolution improving material, since the oil was not decomposed, it can be seen that the oil-degrading rate can be improved by the synergistic effect of the oil-resolution improving material and the oil-degrading bacteria. .

1 wastewater treatment tank,
2a, 2b, 2c partition plate,
3 Receiving residue
4 trap tubes,
5 Water surface,
6 oil,
10 Grease strap.

Claims (7)

Possess the oil resolution enhancement material inorganic powder is coated with a silicone compound, the contacting and oil degrading bacteria, the drainage,
The oil-degrading bacterium is in the form of a live bacterial preparation,
The viable bacterial preparation comprises an oil-decomposing bacterium-containing layer formed on a porous carrier,
The oil-decomposing bacteria-containing layer, as dry cells, 1 part by weight of the oil-decomposing bacteria, 0.8 parts by weight or more and less than 1.5 parts by weight of trehalose, 0.01-5 parts by weight of milk protein, A method for treating wastewater, comprising 0.03 to 10 parts by weight of an amino acid and 0.02 to 10 parts by weight of a metal salt .   The method for treating waste water according to claim 1, wherein the average particle diameter of the oil content resolution improving material is 0.1 to 100 m.   The method for treating wastewater according to claim 1 or 2, wherein the inorganic powder is silica gel.   The silicone compound is at least one selected from the group consisting of methyl hydrogen silicone oil, alkoxy-modified silicone oil, amino-modified silicone oil, epoxy-modified silicone oil, polyether-modified silicone oil, and carboxyl-modified silicone oil. Item 4. The method for treating wastewater according to any one of Items 1 to 3.   The oil-degrading bacterium is a yeast selected from the group consisting of Yarrowia, Candida, Pichia, Hansenula, Saccharomyces, Kluyveromyces, and Trichosporon. 2. The method for treating wastewater according to claim 1. The method for treating wastewater according to claim 5, wherein the oil-degrading bacterium is Yarrowia lipolytica. And oil increased resolution material inorganic powder is coated with a silicone compound, and oil degrading bacteria, only including,
The oil-degrading bacterium is in the form of a live bacterial preparation,
The viable bacterial preparation comprises an oil-decomposing bacterium-containing layer formed on a porous carrier,
The oil-decomposing bacteria-containing layer, as dry cells, 1 part by weight of the oil-decomposing bacteria, 0.8 parts by weight or more and less than 1.5 parts by weight of trehalose, 0.01-5 parts by weight of milk protein, A wastewater treatment kit comprising 0.03 to 10 parts by weight of an amino acid and 0.02 to 10 parts by weight of a metal salt .