JP6920089B2 - Production method of live bacterial product, live bacterial product and wastewater treatment method using it - Google Patents
Production method of live bacterial product, live bacterial product and wastewater treatment method using it Download PDFInfo
- Publication number
- JP6920089B2 JP6920089B2 JP2017070752A JP2017070752A JP6920089B2 JP 6920089 B2 JP6920089 B2 JP 6920089B2 JP 2017070752 A JP2017070752 A JP 2017070752A JP 2017070752 A JP2017070752 A JP 2017070752A JP 6920089 B2 JP6920089 B2 JP 6920089B2
- Authority
- JP
- Japan
- Prior art keywords
- bacterial preparation
- live bacterial
- acid
- oil
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001580 bacterial effect Effects 0.000 title claims description 78
- 238000004519 manufacturing process Methods 0.000 title claims description 25
- 238000004065 wastewater treatment Methods 0.000 title claims description 21
- 238000002360 preparation method Methods 0.000 claims description 85
- 239000007788 liquid Substances 0.000 claims description 64
- 239000002351 wastewater Substances 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 36
- 238000003860 storage Methods 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 241000235015 Yarrowia lipolytica Species 0.000 claims description 19
- 150000003839 salts Chemical class 0.000 claims description 19
- 229910052751 metal Chemical class 0.000 claims description 18
- 239000002184 metal Chemical class 0.000 claims description 17
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims description 15
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims description 15
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims description 15
- 239000005642 Oleic acid Substances 0.000 claims description 15
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims description 15
- 150000001413 amino acids Chemical class 0.000 claims description 15
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims description 15
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 15
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 13
- 102000014171 Milk Proteins Human genes 0.000 claims description 13
- 108010011756 Milk Proteins Proteins 0.000 claims description 13
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 13
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 13
- 235000021239 milk protein Nutrition 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 11
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
- 239000005909 Kieselgur Substances 0.000 claims description 7
- 239000004471 Glycine Substances 0.000 claims description 5
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 238000005469 granulation Methods 0.000 claims description 5
- 230000003179 granulation Effects 0.000 claims description 5
- 229910052749 magnesium Inorganic materials 0.000 claims description 5
- 229910052700 potassium Inorganic materials 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 3
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 description 72
- 239000002609 medium Substances 0.000 description 51
- 239000003921 oil Substances 0.000 description 49
- 235000019198 oils Nutrition 0.000 description 49
- 244000005700 microbiome Species 0.000 description 35
- 210000004027 cell Anatomy 0.000 description 34
- 239000004519 grease Substances 0.000 description 28
- 239000000203 mixture Substances 0.000 description 25
- 125000004432 carbon atom Chemical group C* 0.000 description 21
- 238000009472 formulation Methods 0.000 description 21
- 235000002639 sodium chloride Nutrition 0.000 description 19
- 235000021281 monounsaturated fatty acids Nutrition 0.000 description 18
- 230000004083 survival effect Effects 0.000 description 18
- 230000000694 effects Effects 0.000 description 16
- -1 metal oxide silicates Chemical class 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 14
- 229940024606 amino acid Drugs 0.000 description 14
- 239000000243 solution Substances 0.000 description 14
- 235000014113 dietary fatty acids Nutrition 0.000 description 13
- 229930195729 fatty acid Natural products 0.000 description 13
- 239000000194 fatty acid Substances 0.000 description 13
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 11
- 108090001060 Lipase Proteins 0.000 description 10
- 102000004882 Lipase Human genes 0.000 description 10
- 239000004367 Lipase Substances 0.000 description 10
- 150000004665 fatty acids Chemical class 0.000 description 10
- 235000019421 lipase Nutrition 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 9
- 239000003925 fat Substances 0.000 description 9
- 239000007921 spray Substances 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 8
- 239000002253 acid Substances 0.000 description 8
- 235000019197 fats Nutrition 0.000 description 8
- 230000000813 microbial effect Effects 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 241000235648 Pichia Species 0.000 description 7
- 241000235346 Schizosaccharomyces Species 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- YWWVWXASSLXJHU-AATRIKPKSA-N (9E)-tetradecenoic acid Chemical compound CCCC\C=C\CCCCCCCC(O)=O YWWVWXASSLXJHU-AATRIKPKSA-N 0.000 description 6
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 229930006000 Sucrose Natural products 0.000 description 6
- 241000235013 Yarrowia Species 0.000 description 6
- 239000011575 calcium Substances 0.000 description 6
- 229940041514 candida albicans extract Drugs 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 239000008101 lactose Substances 0.000 description 6
- 239000011777 magnesium Substances 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 235000020183 skimmed milk Nutrition 0.000 description 6
- 235000010344 sodium nitrate Nutrition 0.000 description 6
- 239000004317 sodium nitrate Substances 0.000 description 6
- 239000005720 sucrose Substances 0.000 description 6
- 230000035899 viability Effects 0.000 description 6
- 239000012138 yeast extract Substances 0.000 description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- 102000007544 Whey Proteins Human genes 0.000 description 5
- 108010046377 Whey Proteins Proteins 0.000 description 5
- 238000005273 aeration Methods 0.000 description 5
- 238000000354 decomposition reaction Methods 0.000 description 5
- 150000002016 disaccharides Chemical class 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical group [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000002518 antifoaming agent Substances 0.000 description 4
- 239000005018 casein Substances 0.000 description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 4
- 235000021240 caseins Nutrition 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- SECPZKHBENQXJG-FPLPWBNLSA-N palmitoleic acid Chemical compound CCCCCC\C=C/CCCCCCCC(O)=O SECPZKHBENQXJG-FPLPWBNLSA-N 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 235000021317 phosphate Nutrition 0.000 description 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
- YWWVWXASSLXJHU-UHFFFAOYSA-N 9E-tetradecenoic acid Natural products CCCCC=CCCCCCCCC(O)=O YWWVWXASSLXJHU-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 3
- WWZKQHOCKIZLMA-UHFFFAOYSA-N Caprylic acid Natural products CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- 241000235649 Kluyveromyces Species 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 241000228143 Penicillium Species 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 235000021355 Stearic acid Nutrition 0.000 description 3
- 241000223230 Trichosporon Species 0.000 description 3
- 239000005862 Whey Substances 0.000 description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 229940091250 magnesium supplement Drugs 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 3
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 3
- 238000005192 partition Methods 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229960003975 potassium Drugs 0.000 description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 3
- 239000003223 protective agent Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 150000004671 saturated fatty acids Chemical class 0.000 description 3
- 239000001488 sodium phosphate Substances 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000008117 stearic acid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- YUFFSWGQGVEMMI-JLNKQSITSA-N (7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCCCC(O)=O YUFFSWGQGVEMMI-JLNKQSITSA-N 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- 241000590020 Achromobacter Species 0.000 description 2
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 2
- 241000223600 Alternaria Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 235000021294 Docosapentaenoic acid Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- 241000223198 Humicola Species 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 241001149698 Lipomyces Species 0.000 description 2
- 108010048733 Lipozyme Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical group [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 235000019482 Palm oil Nutrition 0.000 description 2
- 235000021319 Palmitoleic acid Nutrition 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- 235000019484 Rapeseed oil Nutrition 0.000 description 2
- 241000235527 Rhizopus Species 0.000 description 2
- 241000223252 Rhodotorula Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000736131 Sphingomonas Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 241000223259 Trichoderma Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 235000003704 aspartic acid Nutrition 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 235000014121 butter Nutrition 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical group [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- SECPZKHBENQXJG-UHFFFAOYSA-N cis-palmitoleic acid Natural products CCCCCCC=CCCCCCCCC(O)=O SECPZKHBENQXJG-UHFFFAOYSA-N 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- XZTWHWHGBBCSMX-UHFFFAOYSA-J dimagnesium;phosphonato phosphate Chemical compound [Mg+2].[Mg+2].[O-]P([O-])(=O)OP([O-])([O-])=O XZTWHWHGBBCSMX-UHFFFAOYSA-J 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 150000004820 halides Chemical class 0.000 description 2
- KEMQGTRYUADPNZ-UHFFFAOYSA-N heptadecanoic acid Chemical compound CCCCCCCCCCCCCCCCC(O)=O KEMQGTRYUADPNZ-UHFFFAOYSA-N 0.000 description 2
- DCAYPVUWAIABOU-UHFFFAOYSA-N hexadecane Chemical compound CCCCCCCCCCCCCCCC DCAYPVUWAIABOU-UHFFFAOYSA-N 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 150000004677 hydrates Chemical class 0.000 description 2
- VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- YAQXGBBDJYBXKL-UHFFFAOYSA-N iron(2+);1,10-phenanthroline;dicyanide Chemical compound [Fe+2].N#[C-].N#[C-].C1=CN=C2C3=NC=CC=C3C=CC2=C1.C1=CN=C2C3=NC=CC=C3C=CC2=C1 YAQXGBBDJYBXKL-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- FCCDDURTIIUXBY-UHFFFAOYSA-N lipoamide Chemical compound NC(=O)CCCCC1CCSS1 FCCDDURTIIUXBY-UHFFFAOYSA-N 0.000 description 2
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical group [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- 229910000000 metal hydroxide Inorganic materials 0.000 description 2
- 229910044991 metal oxide Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000004006 olive oil Substances 0.000 description 2
- 235000008390 olive oil Nutrition 0.000 description 2
- 239000002540 palm oil Substances 0.000 description 2
- 239000000825 pharmaceutical preparation Substances 0.000 description 2
- 229940127557 pharmaceutical product Drugs 0.000 description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 235000011056 potassium acetate Nutrition 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000005180 public health Effects 0.000 description 2
- 238000010079 rubber tapping Methods 0.000 description 2
- 235000003441 saturated fatty acids Nutrition 0.000 description 2
- 239000008159 sesame oil Substances 0.000 description 2
- 235000011803 sesame oil Nutrition 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- 239000012453 solvate Substances 0.000 description 2
- 235000012424 soybean oil Nutrition 0.000 description 2
- 239000003549 soybean oil Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 235000012222 talc Nutrition 0.000 description 2
- SZHOJFHSIKHZHA-UHFFFAOYSA-N tridecanoic acid Chemical compound CCCCCCCCCCCCC(O)=O SZHOJFHSIKHZHA-UHFFFAOYSA-N 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 235000021119 whey protein Nutrition 0.000 description 2
- 239000007218 ym medium Substances 0.000 description 2
- BITHHVVYSMSWAG-KTKRTIGZSA-N (11Z)-icos-11-enoic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCC(O)=O BITHHVVYSMSWAG-KTKRTIGZSA-N 0.000 description 1
- XSXIVVZCUAHUJO-HZJYTTRNSA-N (11Z,14Z)-icosadienoic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCCCC(O)=O XSXIVVZCUAHUJO-HZJYTTRNSA-N 0.000 description 1
- GEHPRJRWZDWFBJ-FOCLMDBBSA-N (2E)-2-heptadecenoic acid Chemical compound CCCCCCCCCCCCCC\C=C\C(O)=O GEHPRJRWZDWFBJ-FOCLMDBBSA-N 0.000 description 1
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- SZQQHKQCCBDXCG-BAHYSTIISA-N (2e,4e,6e)-hexadeca-2,4,6-trienoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C(O)=O SZQQHKQCCBDXCG-BAHYSTIISA-N 0.000 description 1
- FPRKGXIOSIUDSE-SYACGTDESA-N (2z,4z,6z,8z)-docosa-2,4,6,8-tetraenoic acid Chemical compound CCCCCCCCCCCCC\C=C/C=C\C=C/C=C\C(O)=O FPRKGXIOSIUDSE-SYACGTDESA-N 0.000 description 1
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- HVGRZDASOHMCSK-UHFFFAOYSA-N (Z,Z)-13,16-docosadienoic acid Natural products CCCCCC=CCC=CCCCCCCCCCCCC(O)=O HVGRZDASOHMCSK-UHFFFAOYSA-N 0.000 description 1
- ULNRTPCFRBIMKL-GHVJWSGMSA-N (e)-2-tetracosenoic acid Chemical compound CCCCCCCCCCCCCCCCCCCCC\C=C\C(O)=O ULNRTPCFRBIMKL-GHVJWSGMSA-N 0.000 description 1
- ATNNLHXCRAAGJS-QZQOTICOSA-N (e)-docos-2-enoic acid Chemical compound CCCCCCCCCCCCCCCCCCC\C=C\C(O)=O ATNNLHXCRAAGJS-QZQOTICOSA-N 0.000 description 1
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 1
- GYSCBCSGKXNZRH-UHFFFAOYSA-N 1-benzothiophene-2-carboxamide Chemical compound C1=CC=C2SC(C(=O)N)=CC2=C1 GYSCBCSGKXNZRH-UHFFFAOYSA-N 0.000 description 1
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- LCPVQAHEFVXVKT-UHFFFAOYSA-N 2-(2,4-difluorophenoxy)pyridin-3-amine Chemical compound NC1=CC=CN=C1OC1=CC=C(F)C=C1F LCPVQAHEFVXVKT-UHFFFAOYSA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- WXBXVVIUZANZAU-UHFFFAOYSA-N 2E-decenoic acid Natural products CCCCCCCC=CC(O)=O WXBXVVIUZANZAU-UHFFFAOYSA-N 0.000 description 1
- FPAQLJHSZVFKES-UHFFFAOYSA-N 5-Eicosenoic acid Natural products CCCCCCCCCCCCCCC=CCCCC(O)=O FPAQLJHSZVFKES-UHFFFAOYSA-N 0.000 description 1
- 241000235389 Absidia Species 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 241000589291 Acinetobacter Species 0.000 description 1
- 241000607534 Aeromonas Species 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 241000422409 Alkalibacterium Species 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 241000223679 Beauveria Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical class OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000222511 Coprinus Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- GHVNFZFCNZKVNT-UHFFFAOYSA-N Decanoic acid Natural products CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 1
- 235000021292 Docosatetraenoic acid Nutrition 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 102000008192 Lactoglobulins Human genes 0.000 description 1
- 108010060630 Lactoglobulins Proteins 0.000 description 1
- 239000005639 Lauric acid Substances 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 101710098556 Lipase A Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 101710099648 Lysosomal acid lipase/cholesteryl ester hydrolase Proteins 0.000 description 1
- 102100026001 Lysosomal acid lipase/cholesteryl ester hydrolase Human genes 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241000235575 Mortierella Species 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 101710096328 Phospholipase A2 Proteins 0.000 description 1
- 102100026918 Phospholipase A2 Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241000235402 Rhizomucor Species 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 235000019774 Rice Bran oil Nutrition 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 241000122971 Stenotrophomonas Species 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 235000019486 Sunflower oil Nutrition 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 description 1
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- UWHZIFQPPBDJPM-FPLPWBNLSA-M Vaccenic acid Natural products CCCCCC\C=C/CCCCCCCCCC([O-])=O UWHZIFQPPBDJPM-FPLPWBNLSA-M 0.000 description 1
- 235000021322 Vaccenic acid Nutrition 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000202221 Weissella Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- AZFNGPAYDKGCRB-XCPIVNJJSA-M [(1s,2s)-2-amino-1,2-diphenylethyl]-(4-methylphenyl)sulfonylazanide;chlororuthenium(1+);1-methyl-4-propan-2-ylbenzene Chemical compound [Ru+]Cl.CC(C)C1=CC=C(C)C=C1.C1=CC(C)=CC=C1S(=O)(=O)[N-][C@@H](C=1C=CC=CC=1)[C@@H](N)C1=CC=CC=C1 AZFNGPAYDKGCRB-XCPIVNJJSA-M 0.000 description 1
- 150000001242 acetic acid derivatives Chemical class 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- AHANXAKGNAKFSK-PDBXOOCHSA-N all-cis-icosa-11,14,17-trienoic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCCCC(O)=O AHANXAKGNAKFSK-PDBXOOCHSA-N 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 150000003862 amino acid derivatives Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- LFYJSSARVMHQJB-QIXNEVBVSA-N bakuchiol Chemical compound CC(C)=CCC[C@@](C)(C=C)\C=C\C1=CC=C(O)C=C1 LFYJSSARVMHQJB-QIXNEVBVSA-N 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- GONOPSZTUGRENK-UHFFFAOYSA-N benzyl(trichloro)silane Chemical compound Cl[Si](Cl)(Cl)CC1=CC=CC=C1 GONOPSZTUGRENK-UHFFFAOYSA-N 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 235000001046 cacaotero Nutrition 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 235000011092 calcium acetate Nutrition 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 description 1
- 239000001354 calcium citrate Substances 0.000 description 1
- QXDMQSPYEZFLGF-UHFFFAOYSA-L calcium oxalate Chemical compound [Ca+2].[O-]C(=O)C([O-])=O QXDMQSPYEZFLGF-UHFFFAOYSA-L 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- GBAOBIBJACZTNA-UHFFFAOYSA-L calcium sulfite Chemical group [Ca+2].[O-]S([O-])=O GBAOBIBJACZTNA-UHFFFAOYSA-L 0.000 description 1
- 235000010261 calcium sulphite Nutrition 0.000 description 1
- FAYYUXPSKDFLEC-UHFFFAOYSA-L calcium;dioxido-oxo-sulfanylidene-$l^{6}-sulfane Chemical group [Ca+2].[O-]S([O-])(=O)=S FAYYUXPSKDFLEC-UHFFFAOYSA-L 0.000 description 1
- 239000000828 canola oil Substances 0.000 description 1
- 235000019519 canola oil Nutrition 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000006229 carbon black Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001805 chlorine compounds Chemical class 0.000 description 1
- GWHCXVQVJPWHRF-UHFFFAOYSA-N cis-tetracosenoic acid Natural products CCCCCCCCC=CCCCCCCCCCCCCCC(O)=O GWHCXVQVJPWHRF-UHFFFAOYSA-N 0.000 description 1
- 229910052570 clay Inorganic materials 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- FGRVOLIFQGXPCT-UHFFFAOYSA-L dipotassium;dioxido-oxo-sulfanylidene-$l^{6}-sulfane Chemical compound [K+].[K+].[O-]S([O-])(=O)=S FGRVOLIFQGXPCT-UHFFFAOYSA-L 0.000 description 1
- IRXRGVFLQOSHOH-UHFFFAOYSA-L dipotassium;oxalate Chemical compound [K+].[K+].[O-]C(=O)C([O-])=O IRXRGVFLQOSHOH-UHFFFAOYSA-L 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- CVCXSNONTRFSEH-UHFFFAOYSA-N docosa-2,4-dienoic acid Chemical compound CCCCCCCCCCCCCCCCCC=CC=CC(O)=O CVCXSNONTRFSEH-UHFFFAOYSA-N 0.000 description 1
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 1
- 229940090949 docosahexaenoic acid Drugs 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- FBPFZTCFMRRESA-GUCUJZIJSA-N galactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-GUCUJZIJSA-N 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 description 1
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 1
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 1
- 229960002733 gamolenic acid Drugs 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- YEXZTHNGURCFSJ-UHFFFAOYSA-N henicosa-2,4,6,8,10-pentaenoic acid Chemical compound CCCCCCCCCCC=CC=CC=CC=CC=CC(O)=O YEXZTHNGURCFSJ-UHFFFAOYSA-N 0.000 description 1
- MNWFXJYAOYHMED-UHFFFAOYSA-N heptanoic acid Chemical compound CCCCCCC(O)=O MNWFXJYAOYHMED-UHFFFAOYSA-N 0.000 description 1
- OOJGMLFHAQOYIL-UHFFFAOYSA-N hexadeca-2,4-dienoic acid Chemical compound CCCCCCCCCCCC=CC=CC(O)=O OOJGMLFHAQOYIL-UHFFFAOYSA-N 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 150000003840 hydrochlorides Chemical class 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 1
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 1
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 1
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 1
- IQLUYYHUNSSHIY-UHFFFAOYSA-N icosa-2,4,6,8-tetraenoic acid Chemical compound CCCCCCCCCCCC=CC=CC=CC=CC(O)=O IQLUYYHUNSSHIY-UHFFFAOYSA-N 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 229940119170 jojoba wax Drugs 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000010806 kitchen waste Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000007561 laser diffraction method Methods 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical group [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011654 magnesium acetate Chemical group 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
- 239000001095 magnesium carbonate Substances 0.000 description 1
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 229960001708 magnesium carbonate Drugs 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000004337 magnesium citrate Chemical group 0.000 description 1
- 229960005336 magnesium citrate Drugs 0.000 description 1
- 235000002538 magnesium citrate Nutrition 0.000 description 1
- UHNWOJJPXCYKCG-UHFFFAOYSA-L magnesium oxalate Chemical group [Mg+2].[O-]C(=O)C([O-])=O UHNWOJJPXCYKCG-UHFFFAOYSA-L 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 229960003390 magnesium sulfate Drugs 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940062135 magnesium thiosulfate Drugs 0.000 description 1
- QQFLQYOOQVLGTQ-UHFFFAOYSA-L magnesium;dihydrogen phosphate Chemical compound [Mg+2].OP(O)([O-])=O.OP(O)([O-])=O QQFLQYOOQVLGTQ-UHFFFAOYSA-L 0.000 description 1
- AAJBNRZDTJPMTJ-UHFFFAOYSA-L magnesium;dinitrite Chemical group [Mg+2].[O-]N=O.[O-]N=O AAJBNRZDTJPMTJ-UHFFFAOYSA-L 0.000 description 1
- TZKHCTCLSRVZEY-UHFFFAOYSA-L magnesium;dioxido-oxo-sulfanylidene-$l^{6}-sulfane Chemical compound [Mg+2].[O-]S([O-])(=O)=S TZKHCTCLSRVZEY-UHFFFAOYSA-L 0.000 description 1
- JESHZQPNPCJVNG-UHFFFAOYSA-L magnesium;sulfite Chemical compound [Mg+2].[O-]S([O-])=O JESHZQPNPCJVNG-UHFFFAOYSA-L 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000013310 margarine Nutrition 0.000 description 1
- 239000003264 margarine Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 150000004692 metal hydroxides Chemical class 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- HOVAGTYPODGVJG-ZFYZTMLRSA-N methyl alpha-D-glucopyranoside Chemical compound CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HOVAGTYPODGVJG-ZFYZTMLRSA-N 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000021243 milk fat Nutrition 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N n-hexanoic acid Natural products CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 239000010461 other edible oil Substances 0.000 description 1
- 229940039748 oxalate Drugs 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000003346 palm kernel oil Substances 0.000 description 1
- 235000019865 palm kernel oil Nutrition 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- RAFRTSDUWORDLA-UHFFFAOYSA-N phenyl 3-chloropropanoate Chemical compound ClCCC(=O)OC1=CC=CC=C1 RAFRTSDUWORDLA-UHFFFAOYSA-N 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 235000007686 potassium Nutrition 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 229960002816 potassium chloride Drugs 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010289 potassium nitrite Nutrition 0.000 description 1
- 239000004304 potassium nitrite Substances 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- BHZRJJOHZFYXTO-UHFFFAOYSA-L potassium sulfite Chemical compound [K+].[K+].[O-]S([O-])=O BHZRJJOHZFYXTO-UHFFFAOYSA-L 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 235000019252 potassium sulphite Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 239000008262 pumice Substances 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000008165 rice bran oil Substances 0.000 description 1
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- NNNVXFKZMRGJPM-KHPPLWFESA-N sapienic acid Chemical compound CCCCCCCCC\C=C/CCCCC(O)=O NNNVXFKZMRGJPM-KHPPLWFESA-N 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000011083 sodium citrates Nutrition 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- CSMWJXBSXGUPGY-UHFFFAOYSA-L sodium dithionate Chemical compound [Na+].[Na+].[O-]S(=O)(=O)S([O-])(=O)=O CSMWJXBSXGUPGY-UHFFFAOYSA-L 0.000 description 1
- ZNCPFRVNHGOPAG-UHFFFAOYSA-L sodium oxalate Chemical compound [Na+].[Na+].[O-]C(=O)C([O-])=O ZNCPFRVNHGOPAG-UHFFFAOYSA-L 0.000 description 1
- 229940039790 sodium oxalate Drugs 0.000 description 1
- CHQMHPLRPQMAMX-UHFFFAOYSA-L sodium persulfate Substances [Na+].[Na+].[O-]S(=O)(=O)OOS([O-])(=O)=O CHQMHPLRPQMAMX-UHFFFAOYSA-L 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000002600 sunflower oil Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- JBQYATWDVHIOAR-UHFFFAOYSA-N tellanylidenegermanium Chemical compound [Te]=[Ge] JBQYATWDVHIOAR-UHFFFAOYSA-N 0.000 description 1
- TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-L thiosulfate(2-) Chemical compound [O-]S([S-])(=O)=O DHCDFWKWKRSZHF-UHFFFAOYSA-L 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- 235000019149 tocopherols Nutrition 0.000 description 1
- WXBXVVIUZANZAU-CMDGGOBGSA-N trans-2-decenoic acid Chemical compound CCCCCCC\C=C\C(O)=O WXBXVVIUZANZAU-CMDGGOBGSA-N 0.000 description 1
- HOGWBMWOBRRKCD-BUHFOSPRSA-N trans-2-pentadecenoic acid Chemical compound CCCCCCCCCCCC\C=C\C(O)=O HOGWBMWOBRRKCD-BUHFOSPRSA-N 0.000 description 1
- UWHZIFQPPBDJPM-BQYQJAHWSA-N trans-vaccenic acid Chemical compound CCCCCC\C=C\CCCCCCCCCC(O)=O UWHZIFQPPBDJPM-BQYQJAHWSA-N 0.000 description 1
- 235000013337 tricalcium citrate Nutrition 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- PLSARIKBYIPYPF-UHFFFAOYSA-H trimagnesium dicitrate Chemical group [Mg+2].[Mg+2].[Mg+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O PLSARIKBYIPYPF-UHFFFAOYSA-H 0.000 description 1
- 235000019798 tripotassium phosphate Nutrition 0.000 description 1
- 229910000404 tripotassium phosphate Inorganic materials 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229910000406 trisodium phosphate Inorganic materials 0.000 description 1
- 235000019801 trisodium phosphate Nutrition 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 238000007601 warm air drying Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000010698 whale oil Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- QUEDXNHFTDJVIY-UHFFFAOYSA-N γ-tocopherol Chemical class OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-UHFFFAOYSA-N 0.000 description 1
Images
Description
本発明は、生菌製剤の製造方法、ならびに生菌製剤およびこれを用いた排水処理方法に関する。 The present invention relates to a method for producing a live bacterial preparation, and a live bacterial preparation and a wastewater treatment method using the same.
生きた微生物を有効成分として含む製剤(生菌製剤)は、食品や医薬品分野のみならず、環境、農薬、公衆衛生など、産業界において広範囲で応用が検討されている。 Pharmaceuticals containing live microorganisms as active ingredients (live bacterial preparations) are being studied for a wide range of applications not only in the food and pharmaceutical fields, but also in the environment, pesticides, public health, and other industries.
生菌製剤の利用方法の一つとして、排水処理技術への応用が検討されており、一例としては、油分分解能を有する微生物のグリーストラップへの利用がある。厨房や食品工場などの施設において、油分を集積して上層部に浮上した油分を分離して廃棄するためのグリーストラップが敷設されている。グリーストラップに油分分解能を有する微生物を投入することにより、油分の分解・資化を行うことができる。これにより、固形化した油分がグリーストラップの水面にスカム(油の塊)として残留したり、グリーストラップの内壁面や配管内部に集積・付着して配管を閉塞したりすることを低コストで予防できる。このため、油分の酸化・腐敗による悪臭・害虫の発生の防止方法として期待されている。かような油分分解能を有する微生物の一つとして、特許文献1には、ヤロウィア・リポリティカ(Yarrowia lipolytica)に属し、pH3.5〜10.5の条件で、1%(w/v)の油分を20時間で50重量%以上低減する、微生物が開示されている。
As one of the methods of using the live bacterial preparation, its application to wastewater treatment technology is being studied, and one example is the use of a microorganism having oil resolution in a grease trap. In facilities such as kitchens and food factories, grease traps are installed to collect oil and separate and dispose of the oil that has floated on the upper layer. By putting a microorganism having an oil content resolution into the grease trap, the oil content can be decomposed and assimilated. This prevents solidified oil from remaining on the water surface of the grease trap as a scum (lump of oil) or accumulating and adhering to the inner wall surface of the grease trap or inside the pipe and blocking the pipe at low cost. can. Therefore, it is expected as a method for preventing the generation of foul odors and pests due to oxidation and putrefaction of oil. As one of the microorganisms having such oil content resolution,
生菌製剤に関する問題点として、製剤化工程や製品の保存中に微生物の生存率が低下するという技術的課題が存在していた。このような問題に対し、微生物農薬組成物中の芽胞を長期間安定して、生存した状態で保存する技術として、特許文献2には、バシルス・サチリス及び/又はバシルス・アミロリケファシエンスの生芽胞と金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される少なくとも1種以上を配合してなる微生物農薬組成物が記載されている。
As a problem with the live bacterial preparation, there is a technical problem that the survival rate of microorganisms is lowered during the formulation process and the storage of the product. In response to such problems, as a technique for preserving spores in a microbial pesticide composition in a stable and alive state for a long period of time,
芽胞を形成する微生物は、芽胞を利用することによって過酷な環境においても生存する能力に長けているため、優れた耐熱性や乾燥耐性などを示す。 Microorganisms that form spores excel in the ability to survive in harsh environments by using spores, and thus exhibit excellent heat resistance and drought resistance.
しかしながら、特許文献1に記載されるような芽胞を形成しない微生物(無芽胞菌)の場合、芽胞を形成する微生物に比べて耐熱性や乾燥耐性に優れていないため、その後の保存期間中、特に高温条件下での保存期間中に生存率が顕著に低下する場合が存在していた。このため、特許文献2に記載されるような芽胞を形成する微生物向けの製剤化技術では、依然として保存中における、特に高温下での無芽胞菌の生存率を十分に高くできない場合が存在していた。
However, microorganisms that do not form spores (spore-free bacteria) as described in
したがって、本発明は、上記事情を鑑みてなされたものであり、高温保存下における微生物の生存率が向上された、無芽胞菌を含む生菌製剤の製造方法を提供することを課題とする。 Therefore, the present invention has been made in view of the above circumstances, and an object of the present invention is to provide a method for producing a viable bacterial preparation containing spore-free bacteria, which has an improved survival rate of microorganisms under high-temperature storage.
本発明者らは、上記の問題を解決すべく鋭意研究を行った。その結果、炭素数14〜18のモノ不飽和脂肪酸を含む培地で培養した無芽胞菌および水を含む付着液を、多孔質担体に付着させることを含む、生菌製剤の製造方法とすることで上記課題が解決されることを見出し、本発明の完成に至った。 The present inventors have conducted diligent research to solve the above problems. As a result, it is possible to obtain a method for producing a viable bacterial preparation, which comprises adhering an adherent solution containing spore-free bacteria and water cultured in a medium containing monounsaturated fatty acids having 14 to 18 carbon atoms to a porous carrier. We have found that the above problems can be solved, and have completed the present invention.
本発明の製造方法によれば、生菌製剤が高温下にて保存された場合であっても無芽胞菌の生菌率が高いものとなる。 According to the production method of the present invention, the viable cell ratio of spore-free bacteria is high even when the viable bacterial preparation is stored at a high temperature.
本発明の一実施形態は、炭素数14〜18のモノ不飽和脂肪酸を含む培地で培養した無芽胞菌および水を含む付着液を、多孔質担体に付着させることを含む、生菌製剤の製造方法である。本発明によれば、高温保存下における無芽胞菌の生菌率が向上された、無芽胞菌を含む生菌製剤の製造方法を提供することができる。本発明により上記のような効果が得られる詳細なメカニズムは依然として明確ではないが、以下のように推測される。炭素数14〜18のモノ不飽和脂肪酸が無芽胞菌内に取り込まれることで、細胞内にある炭素間二重結合の生成を触媒する酵素の働きがフィードバック阻害される。これにより、細胞膜を構成する脂肪酸に占める飽和脂肪酸の割合が高まる。その結果、細胞膜の流動性が低下し、膜の強度が高まったため、高温保存下での生菌率が向上したものと考えられる。 One embodiment of the present invention comprises attaching a viable cell preparation to a porous carrier by adhering an adhering solution containing water and spore-free bacteria cultured in a medium containing monounsaturated fatty acids having 14 to 18 carbon atoms. The method. According to the present invention, it is possible to provide a method for producing a viable bacterial preparation containing spore-free bacteria, which has an improved viable cell ratio of spore-free bacteria under high-temperature storage. The detailed mechanism by which the above effects are obtained by the present invention is still unclear, but it is presumed as follows. Incorporation of monounsaturated fatty acids having 14 to 18 carbon atoms into non-spore-forming bacteria inhibits feedback of the action of enzymes that catalyze the formation of intracellular double bonds. This increases the proportion of saturated fatty acids in the fatty acids that make up the cell membrane. As a result, it is considered that the fluidity of the cell membrane decreased and the strength of the membrane increased, so that the viable cell rate under high temperature storage was improved.
以下、本発明の実施の形態を説明する。なお、本発明は、以下の実施の形態のみには限定されない。また、図面の寸法比率は、説明の都合上誇張されており、実際の比率とは異なる場合がある。 Hereinafter, embodiments of the present invention will be described. The present invention is not limited to the following embodiments. In addition, the dimensional ratios in the drawings are exaggerated for convenience of explanation and may differ from the actual ratios.
本明細書において、範囲を示す「X〜Y」は「X以上Y以下」を意味する。また、特記しない限り、操作および物性等の測定は室温(20〜25℃)/相対湿度40〜50%RHの条件で行う。 In the present specification, "X to Y" indicating a range means "X or more and Y or less". Unless otherwise specified, operations and physical properties are measured under the conditions of room temperature (20 to 25 ° C.) / relative humidity of 40 to 50% RH.
<生菌製剤の製造方法>
(1)無芽胞菌の培養
本実施形態では、無芽胞菌を炭素数14〜18のモノ不飽和脂肪酸を含む培地で培養する。
<Manufacturing method of live bacterial product>
(1) Culturing of non-spore-forming bacteria In this embodiment, the non-spore-forming bacteria are cultured in a medium containing monounsaturated fatty acids having 14 to 18 carbon atoms.
本発明においては種々の無芽胞菌(芽胞非形成菌)を用いることができる。本明細書における「無芽胞菌」には酵母、細菌、かび等が含まれ、例えば、ヤロウィア属(Yarrowia)、キャンディダ属(Candida)、ピキア属(Pichia)、ハンセヌラ属(Hansenura)、サッカロマイセス属(Saccharomyces)、クルイベロマイセス属(Kluyveromyces)、トリコスポロン属(Trichosporon)、リポマイセス属(Lipomyces)、ロードトルラ(Rhodotorula)属、シゾサッカロマイセス属(Schizosaccharomyces)、ラクトバシラス属(Lactobacillus)、エンテロコッカス属(Enter
ococcus)、ラクトコッカス属(Lactococcus)、ビフィドバクテリウム属(Bifidobacterium)、ストレプトコッカス属(Streptococcus)、エンテロバクター属(Enterobacter)、スフィンゴモナス属(Sphingomonas)、バークホルデリア(Burkholderia)属、ウェイセラ属(Weissella)、アシネトバクター属(Acinetobacter)、アクロモバクター属(Achromobacter)、アセトバクター属(Acetobacter)、アルカリゲネス属(Alcaligenes)、アルスロバクター属(Arthrobacter)、コリネバクテリウム属(Corynebacterium)、フラボバクテリウム属(Flavobacterium)、アルカニボラックス属(Alcanivorax)、アルカリバクテリウム属(Alkalibacterium)、ステノトロフォモナス属(Stenotrophomonas)、アスペルギルス属(Aspergillus)、ペニシリウム属(Penicillium)、ムコール属(Mucor)、リゾムコール属(Rhizomucor)、モルティエレラ属(Mortierella)、フザリウム属(Fusarium)、シュードモナス属(Pseudomonas)、リゾプス属(Rhizopus)、フミコラ属(Humicola)、アクレモニウム属(Acremonium)、アエロモナス属(Aeromonas)、ブレビバクテリウム属(Brevibacterium)、トリコデルマ属(Trichoderma)等に属する無芽胞菌が例示できるが、これらに限定されない。無芽胞菌は1種を単独で、または2種以上を混合して用いることができる。本発明においては、無芽胞菌として酵母が好適に用いられる。
In the present invention, various non-spore-forming bacteria (non-spore-forming bacteria) can be used. The "blastless bacteria" in the present specification include yeasts, bacteria, molds and the like, and include, for example, the genus Yarrowia, the genus Candida, the genus Pichia, the genus Hansenura, and the genus Schizosaccharomyces. (Saccharomyces), genus Kluyveromyces, genus Trichosporon, genus Lipomyces, genus Rhodotorula, genus Schizosaccharomyces, genus Schizosaccharomyces, genus Schizosaccharomyces
ococcus, Lactococcus, Bifidobacterium, Streptococcus, Enterobacter, Sphingomonas, Sphingomonas, Berkuhor Weissella), Acinetobacter, Achromobacter, Acetobacter, Alcaligenes, Artrobacter, Corynebacter, Corynebacter (Flavobactium), Arcanivorax, Alkalibacterium, Stenotrophomonas, Aspergillus, Penicillium, Penicillium (Rhizomucor), Mortierella, Fusarium, Pseudomonas, Rhizopus, Humicola, Acremonium, Acremonium Examples include, but are not limited to, non-spore-forming bacteria belonging to the genus Brevibacterium, the genus Trichoderma, and the like. As the spore-free bacterium, one kind may be used alone, or two or more kinds may be used in combination. In the present invention, yeast is preferably used as the spore-free bacterium.
後述の排水処理方法に生菌製剤を用いる場合には、無芽胞菌としては、ヤロウィア属(Yarrowia)、キャンディダ属(Candida)、ピキア属(Pichia)、ハンセヌラ属(Hansenura)、サッカロマイセス属(Saccharomyces)、クルイベロマイセス属(Kluyveromyces)、トリコスポロン属(Trichosporon)、リポマイセス属(Lipomyces)、ロードトルラ(Rhodotorula)属、シゾサッカロマイセス属(Schizosaccharomyces)、ラクトバシラス属(Lactobacillus)、エンテロコッカス属(Enterococcus)等の酵母が好ましく用いられ、油分分解活性の高さから、ヤロウィア・リポリティカ ATCC48436、ヤロウィア・リポリティカ NBRC1548、ヤロウィア・リポリティカ LM02−011(受託番号NITE P−01813)、ヤロウィア・リポリティカ NBRC0746、ヤロウィア・リポリティカ NBRC1209のようなヤロウィア・リポリティカ(Yarrowia lipolytica)、ヤロウィア YH−01のようなヤロウィア スピーシーズ(Yarrowia
sp.)等のヤロウィア属の酵母であることがより好ましく、ヤロウィア・リポリティカ(Yarrowia lipolytica)であることが好ましく、広範囲のpH領域で高い油分分解活性を有するという観点から、ヤロウィア・リポリティカ LM02−011(受託番号NITE P−01813)が特に好ましい。
When a viable bacterial preparation is used in the wastewater treatment method described later, the as germ-free bacteria include Yarrowia, Candida, Pichia, Hansenura, and Schizosaccharomyces. ), Kluyveromyces, Trichosporon, Lipomyces, Rhodotorula, Schizosaccharomyces, etc., Schizosaccharomyces, Lactobacils, Lactobacils Yeast is preferably used, and due to its high oil decomposition activity, Yarrowia lipolytica ATCC48436, Yarrowia lipolytica NBRC1548, Yarrowia lipolytica LM02-011 (accession number NITE P-01813), Yarrowia lipolytica NBRC0746, Yarrowia lipolytica NBRC0 Yarrowia lipolytica, Yarrowia spices like YH-01
sp. ) And other yeasts of the genus Yarrowia, preferably Yarrowia lipolytica, and from the viewpoint of having high oil decomposition activity in a wide pH range, Yarrowia lipolytica LM02-011 (consignment). Number NITE P-01813) is particularly preferred.
本実施形態では、炭素数14〜18のモノ不飽和脂肪酸を含む培地で無芽胞菌を培養する。炭素数14〜18のモノ不飽和脂肪酸(以下、単にモノ不飽和脂肪酸とも称する)は、二重結合を1つ有する炭素数14〜18の脂肪酸を指す。炭素数14〜18のモノ不飽和脂肪酸のうち、炭素数14〜18のcis型モノ不飽和脂肪酸であることが好ましい。炭素数14〜18のcis型モノ不飽和脂肪酸としては、例えば、炭素数14のcis−9−モノ不飽和脂肪酸であるミリストレイン酸、炭素数16のcis−6−モノ不飽和脂肪酸であるサピエン酸、炭素数16のcis−9−モノ不飽和脂肪酸であるパルミトレイン酸、炭素数18のcis−9−モノ不飽和脂肪酸であるオレイン酸、炭素数18のcis−11−モノ不飽和脂肪酸であるバクセン酸などが挙げられる。本発明の効果が一層奏されることから、モノ不飽和脂肪酸としては、炭素数14〜18のcis型−9−モノ不飽和脂肪酸であることが好ましく、具体的には、ミリストレイン酸、パルミトレイン酸、
およびオレイン酸であることが好ましく、オレイン酸であることがより好ましい。炭素数14〜18のモノ不飽和脂肪酸は、1種単独でも2種以上併用してもよい。
In this embodiment, spore-free bacteria are cultured in a medium containing monounsaturated fatty acids having 14 to 18 carbon atoms. A monounsaturated fatty acid having 14 to 18 carbon atoms (hereinafter, also simply referred to as a monounsaturated fatty acid) refers to a fatty acid having 14 to 18 carbon atoms having one double bond. Of the monounsaturated fatty acids having 14 to 18 carbon atoms, cis-type monounsaturated fatty acids having 14 to 18 carbon atoms are preferable. Examples of the cis-type monounsaturated fatty acid having 14 to 18 carbon atoms include myristoleic acid, which is a cis-9-mono-unsaturated fatty acid having 14 carbon atoms, and sapienic acid, which is a cis-6-mono-unsaturated fatty acid having 16 carbon atoms. Acids, palmitoleic acid, which is a cis-9-monounsaturated fatty acid with 16 carbon atoms, oleic acid, which is a cis-9-monounsaturated fatty acid with 18 carbon atoms, and cis-11-monounsaturated fatty acid, which has 18 carbon atoms. Examples include vaccenic acid. Since the effects of the present invention are further exhibited, the monounsaturated fatty acid is preferably a cis-type-9-monounsaturated fatty acid having 14 to 18 carbon atoms, and specifically, myristoleic acid and palmitoleic acid. acid,
And oleic acid, more preferably oleic acid. The monounsaturated fatty acids having 14 to 18 carbon atoms may be used alone or in combination of two or more.
培地中におけるモノ不飽和脂肪酸の含有量は、本発明の効果が一層奏されることから、液体培地の場合、10μg/mL以上であることが好ましく、15μg/mL以上であることがより好ましく、20μg/mL以上であることがさらに好ましい。培地中におけるモノ不飽和脂肪酸の含有量の上限は特に制限されるものではないが、効果が飽和し、添加に見合った効果が得られなくなることから、150μg/mL以下であることが好ましく、100μg/mL以下であることがより好ましい。 The content of the monounsaturated fatty acid in the medium is preferably 10 μg / mL or more, more preferably 15 μg / mL or more in the case of a liquid medium, because the effect of the present invention is further exhibited. It is more preferably 20 μg / mL or more. The upper limit of the content of monounsaturated fatty acids in the medium is not particularly limited, but it is preferably 150 μg / mL or less, preferably 100 μg, because the effect is saturated and the effect commensurate with the addition cannot be obtained. More preferably, it is / mL or less.
また、培地の他の成分としては、使用する微生物が資化しうる炭素源、適量の窒素源、無機塩及びその他の栄養素が挙げられる。 In addition, other components of the medium include carbon sources that can be assimilated by the microorganisms used, appropriate amounts of nitrogen sources, inorganic salts and other nutrients.
無芽胞菌の培養において使用できる炭素源としては、使用する菌株が資化できる炭素源であれば特に制限されない。具体的には、微生物の資化性を考慮して、グルコース、フラクトース、セロビオース、ラフィノース、キシロース、マルトース、ガラクトース、ソルボース、グルコサミン、リボース、アラビノース、ラムノース、スクロース、α−メチル−D−グルコシド、サリシン、メリビオース、ラクトース、メレジトース、イヌリン、エリスリトール、グルシトール、マンニトール、ガラクチトール、N−アセチル−D−グルコサミン、デンプン、デンプン加水分解物、糖蜜、廃糖蜜等の糖類、麦、米等の天然物、グリセロール、メタノール、エタノール等のアルコール類、酢酸、乳酸、コハク酸、グルコン酸、ピルピン酸、クエン酸等の有機酸類、ヘキサデカン等の炭化水素などが挙げられる。中でも、炭素源としては、グルコースであることが好ましい。上記炭素源は、培養する無芽胞菌による資化性を考慮して適宜選択される。また、上記炭素源は1種単独で用いてもよいし、2種以上併用してもよい。 The carbon source that can be used in the culture of spore-free bacteria is not particularly limited as long as the strain used can be assimilated. Specifically, considering the assimilation of microorganisms, glucose, fructose, cellobiose, raffinose, xylose, maltose, galactose, sorbose, glucosamine, ribose, arabinose, lambnorth, sucrose, α-methyl-D-glucoside, salicin , Melibiose, lactose, meregitos, inulin, erythritol, glucitol, mannitol, galactitol, N-acetyl-D-glucosamine, starch, starch hydrolysate, sugars such as sucrose and waste sugar, natural products such as wheat and rice, glycerol , Alcohols such as methanol and ethanol, organic acids such as acetic acid, lactic acid, sucrose, gluconic acid, pyruvic acid and citric acid, and hydrocarbons such as hexadecane. Of these, glucose is preferable as the carbon source. The carbon source is appropriately selected in consideration of the assimilation property of the spore-free bacterium to be cultured. Further, the carbon source may be used alone or in combination of two or more.
また、無芽胞菌の培養において使用できる窒素源としては、肉エキス、ペプトン、ポリペプトン、酵母エキス、麦芽エキス、大豆加水分解物、大豆粉末、カゼイン、ミルクカゼイン、カザミノ酸、グリシン、グルタミン酸、アスパラギン酸等の各種アミノ酸、コーンスティープリカー、その他の動物、植物、微生物の加水分解物等の有機窒素源;アンモニア、硝酸アンモニウム、硫酸アンモニウム、塩化アンモニウムなどのアンモニウム塩、硝酸ナトリウムなどの硝酸塩、亜硝酸ナトリウムなどの亜硝酸塩、尿素等の無機窒素源などが挙げられる。上記窒素源は、培養する無芽胞菌による資化性を考慮して適宜選択される。中でも、窒素源は、酵母エキス、麦芽エキス、および硝酸ナトリウムからなる群から選択される少なくとも1種であることがより好ましい。また、上記窒素源は1種単独で用いてもよいし、2種以上併用してもよい。 Nitrogen sources that can be used in the culture of blast-free bacteria include meat extract, peptone, polypeptone, yeast extract, malt extract, soybean hydrolyzate, soybean powder, casein, milk casein, cazamino acid, glycine, glutamate, and aspartic acid. Various amino acids such as corn steep liquor, organic nitrogen sources such as hydrolysates of other animals, plants, microorganisms; ammonium salts such as ammonia, ammonium nitrate, ammonium sulfate, ammonium chloride, nitrates such as sodium nitrate, sodium nitrite, etc. Examples thereof include inorganic nitrogen sources such as nitrite and urea. The nitrogen source is appropriately selected in consideration of the assimilation property of the spore-free bacterium to be cultured. Above all, the nitrogen source is more preferably at least one selected from the group consisting of yeast extract, malt extract, and sodium nitrate. Further, the nitrogen source may be used alone or in combination of two or more.
無芽胞菌の培養に使用できる無機物としては、マグネシウム、マンガン、カルシウム、ナトリウム、カリウム、銅、鉄及び亜鉛などの、リン酸塩、塩酸塩、硫酸塩、酢酸塩、炭酸塩、重炭酸塩、塩化物等のハロゲン化物などが挙げられる。上記無機物は、培養する無芽胞菌による資化性を考慮して適宜選択される。また、上記無機物を1種または2種以上選択して使用することができる。また、培養中に発泡が生じる場合には、公知の各種消泡剤を適宜培地中に添加することもできる。 Inorganic substances that can be used for culturing blast-free bacteria include phosphates, hydrochlorides, sulfates, acetates, carbonates, bicarbonates, such as magnesium, manganese, calcium, sodium, potassium, copper, iron and zinc. Halides such as chlorides can be mentioned. The above-mentioned inorganic substances are appropriately selected in consideration of the assimilation property of the spore-free bacteria to be cultured. Moreover, one kind or two or more kinds of the said inorganic substances can be selected and used. In addition, when foaming occurs during culturing, various known antifoaming agents can be appropriately added to the medium.
培地は、固体または液体培地のいずれでもよく、また、使用する無芽胞菌が資化しうる炭素源、適量の窒素源、無機塩及びその他の栄養素を含有する培地であれば、合成培地または天然培地のいずれでもよい。 The medium may be either a solid or liquid medium, and is a synthetic medium or a natural medium as long as it contains a carbon source, an appropriate amount of nitrogen source, an inorganic salt and other nutrients that can be assimilated by the non-spore-forming bacteria used. It may be any of.
無芽胞菌の培養条件は、当該無芽胞菌が生育・増殖できるものであれば、いずれのものであってよく、通常の方法によって行える。 The culture conditions for the spore-free bacterium may be any as long as the spore-free bacterium can grow and proliferate, and can be carried out by a usual method.
無芽胞菌の種類によって、好気的条件下または嫌気的条件下で、無芽胞菌を培養する。前者の場合には、振とう培養または通気攪拌などによって行われる。通気攪拌培養の場合、通気の量は、培養の容量と時間、および菌の初発濃度を考慮して、適宜決定することができる。例えば、通気は0.2〜2vvm(Volume per volume per minuts)程度で行われる。また、攪拌は50〜800rpm程度で行なうことができる。 Depending on the type of spore-free bacterium, the spore-free bacterium is cultured under aerobic or anaerobic conditions. In the former case, it is carried out by shaking culture or aeration stirring. In the case of aeration stirring culture, the amount of aeration can be appropriately determined in consideration of the volume and time of the culture and the initial concentration of the bacterium. For example, ventilation is performed at about 0.2 to 2 vvm (Volume per volume per minutes). Further, stirring can be performed at about 50 to 800 rpm.
また、無芽胞菌を培養をするにあたって、寒天培地等の固体培地に斜面培養したものを直接培養用の培地に植菌して、培養を行なってもよい(場合によっては、種培養と区別するために「本培養」とも記載する)が、無芽胞菌を予め液体培地で培養(種培養)したものを、さらに培養(本培養)してもよい。この際、種培養の培地についても、炭素数14〜18のモノ不飽和脂肪酸を含む培地を用いることが好ましい。 In addition, when culturing blast-free bacteria, those cultivated on a solid surface such as an agar medium on a slope may be directly inoculated into a medium for culturing and cultured (in some cases, it is distinguished from seed culture). For this reason, it is also referred to as "main culture"), but a non-blastic bacterium that has been previously cultured in a liquid medium (seed culture) may be further cultured (main culture). At this time, it is preferable to use a medium containing monounsaturated fatty acids having 14 to 18 carbon atoms as the medium for seed culture.
また、培養温度は、好ましくは15〜40℃、より好ましくは25〜35℃である。また、培養に適当な培地のpHは、好ましくは3〜11、より好ましくは3.5〜10.5であり、さらに好ましくは、4.0〜8.0である。特に、工業的に培養物を量産する場合には、培地中のpHを定期的に測定し、pH4.0〜8.0に維持するよう調整することが好ましい。培養時間は、無芽胞菌が十分に増殖するまで行えばよいが、例えば、本培養で通常0.5〜5日であり、好ましくは1〜5日である。 The culture temperature is preferably 15 to 40 ° C, more preferably 25 to 35 ° C. The pH of the medium suitable for culturing is preferably 3 to 11, more preferably 3.5 to 10.5, and even more preferably 4.0 to 8.0. In particular, in the case of industrial mass production of cultures, it is preferable to periodically measure the pH in the medium and adjust the pH to be maintained at 4.0 to 8.0. The culturing time may be continued until the spore-free bacterium grows sufficiently. For example, in the main culture, it is usually 0.5 to 5 days, preferably 1 to 5 days.
無芽胞菌を連続的にまたはバッチで培養することができる。 Spore-free bacteria can be cultured continuously or in batches.
なお、無芽胞菌を湿菌体として付着液に用いてもよいし、凍結乾燥やスプレードライ等により乾燥した乾燥菌体として付着液に用いてもよいが、後述する各微生物保護剤の添加量は、無芽胞菌を乾燥菌体に換算した量に対する値である。乾燥菌体に換算した量とは、菌体を乾燥させた後の重量を意味する。乾燥後の菌体重量を求める方法では、例えば、まず、酵母の培養物を遠心分離処理することにより、菌体を沈殿として回収する。回収した菌体を遠心分離操作により2回水洗した後、105℃で5時間乾燥させた後の重量を測定することにより、乾燥菌体重量を求めることができる。 The spore-free bacteria may be used as wet cells in the adhering solution, or may be used as dried cells dried by freeze-drying or spray-drying in the adhering solution, but the amount of each microbial protective agent added will be described later. Is a value with respect to the amount of non-spore-forming bacteria converted into dried cells. The amount converted into dried cells means the weight after drying the cells. In the method for determining the weight of cells after drying, for example, first, the yeast culture is centrifuged to collect the cells as a precipitate. The weight of the dried cells can be determined by measuring the weight of the collected cells after washing them twice with water by a centrifugation operation and then drying them at 105 ° C. for 5 hours.
培養物から無芽胞菌を回収する方法は、特に限定されるものではなく、培養物から無芽胞菌を回収する場合に通常行なわれている回収方法のいずれを用いてもよい。培養物から無芽胞菌を回収する方法として、例えば、培養物を遠心分離処理する方法等が挙げられる。 The method for recovering the spore-free bacterium from the culture is not particularly limited, and any of the recovery methods usually used when recovering the spore-free bacterium from the culture may be used. Examples of the method for recovering spore-free bacteria from the culture include a method for centrifuging the culture.
(2)付着液
本実施形態では、付着液が上記培地で培養された無芽胞菌および水を含む。付着液は多孔質担体に付着される液を指し、好適には噴霧液である。
(2) Adhesive liquid In the present embodiment, the adhering liquid contains spore-free bacteria and water cultured in the above medium. The adhering liquid refers to a liquid adhering to the porous carrier, and is preferably a spray liquid.
上記培地で培養された無芽胞菌を付着液として用いることで、得られる生菌製剤の高温での保存安定性が顕著に向上する。 By using the non-spore-forming bacterium cultured in the above medium as an adhering solution, the storage stability of the obtained viable bacterial preparation at high temperature is remarkably improved.
付着液が含む水は、水道水、蒸留水、イオン交換水、純水、超純水など、いずれを使用してもよいが、不純物の少ない蒸留水、イオン交換水、純水、または超純水を用いることが好ましい。メタノール、エタノール、プロパノール等の低級アルコールや、アセトンなどの極性溶媒を、本発明の目的効果が損なわれない程度において水に添加した混合液を用いてもよい。 The water contained in the adhering liquid may be tap water, distilled water, ion-exchanged water, pure water, ultrapure water, etc., but distilled water with few impurities, ion-exchanged water, pure water, or ultrapure water may be used. It is preferable to use water. A mixed solution in which a lower alcohol such as methanol, ethanol or propanol or a polar solvent such as acetone is added to water to the extent that the objective effect of the present invention is not impaired may be used.
付着液中の無芽胞菌の量は特に制限されないが、例えば、付着液中に乾燥菌体として1
〜20重量%となる量であり、好ましくは5〜15重量%となる量である。
The amount of non-spore-forming bacteria in the adhering liquid is not particularly limited, but for example, 1 as dried cells in the adhering liquid.
It is an amount of about 20% by weight, preferably 5 to 15% by weight.
付着液は好適には、トレハロース、乳タンパク質、アミノ酸、および金属塩を含む。付着液に存在するトレハロース、乳タンパク質、アミノ酸、および金属塩が無芽胞菌の安定化剤として製剤化工程における無芽胞菌に対するダメージを軽減するため、製剤化工程における生存率を高く維持することができる。本明細書において、トレハロース、乳タンパク質、アミノ酸、および金属塩をまとめて微生物保護剤とも称する。 The adhering fluid preferably comprises trehalose, milk protein, amino acids, and metal salts. Trehalose, milk protein, amino acids, and metal salts present in the adhering liquid act as stabilizers for spore-free bacteria to reduce damage to spore-forming bacteria in the formulation process, so that the survival rate in the formulation process can be maintained high. can. In the present specification, trehalose, milk protein, amino acids, and metal salts are also collectively referred to as a microbial protectant.
付着液中のトレハロースの量は、乾燥菌体として1重量部の無芽胞菌に対して、0.8重量部以上1.5重量部未満であることが好ましい。付着液中のトレハロースの量が上記範囲内にあることで、製造化工程直後および生菌製剤の保存期間中の微生物の生存率が向上する。微生物の製剤化工程後における生存率と保存期間中の生存率とのさらなる好適なバランスの観点から、付着液中のトレハロース含有量は、乾燥菌体換算で1重量部の無芽胞菌に対して、好ましくは0.9重量部を超えて1.5重量部未満であり、より好ましくは1重量部以上1.4重量部以下である。 The amount of trehalose in the adhering liquid is preferably 0.8 parts by weight or more and less than 1.5 parts by weight with respect to 1 part by weight of non-spore-forming bacteria as dried mycelia. When the amount of trehalose in the adhering liquid is within the above range, the viability of the microorganism is improved immediately after the production process and during the storage period of the live bacterial preparation. From the viewpoint of a more suitable balance between the survival rate after the microbial formulation step and the survival rate during the storage period, the trehalose content in the adhered solution is based on 1 part by weight of spore-free bacteria in terms of dry cells. It is preferably more than 0.9 parts by weight and less than 1.5 parts by weight, and more preferably 1 part by weight or more and 1.4 parts by weight or less.
なお、本発明においては、トレハロース以外の二糖類、たとえばスクロース、ラクトース、マルトース等のトレハロース以外の二糖類を、1種単独でまたは2種以上を混合して、トレハロースと併用してもよい。この場合、トレハロース以外の二糖類の含有量は、乾燥菌体として1重量部の無芽胞菌に対して、例えば0.01重量部以上0.8重量部未満であり、好ましくは0.1重量部以上0.5重量部以下である。 In the present invention, disaccharides other than trehalose, for example, disaccharides other than trehalose such as sucrose, lactose, and maltose may be used alone or in combination of two or more in combination with trehalose. In this case, the content of the disaccharide other than trehalose is, for example, 0.01 part by weight or more and less than 0.8 part by weight, preferably 0.1 part by weight, based on 1 part by weight of the non-spore-forming bacterium as a dried bacterial cell. Parts or more and 0.5 parts by weight or less.
乳タンパク質としては特に制限されず、従来公知の各種の乳タンパク質を含む材料を用いることができる。全脂粉乳のような乳脂肪分の多い原料を用いることもできるが、グリーストラップで使用するような油脂分解用生菌製剤を製造する場合は、製剤中の油分は少ない方が好ましいので、例えば、好ましい具体例としては、カゼイン、カゼインナトリウム、ホエイ、濃縮ホエイ、ホエイタンパク質濃縮物(WPC)、ホエイタンパク質分離物(WPI)、ホエイパウダー、スキムミルク、乳タンパク濃縮物(MPC)、脱脂粉乳、α−ラクトアルブミン、β−ラクトグロブリン等が例示でき、これらを1種単独でまたは2種以上を混合して用いることができる。乳タンパク質原料としてスキムミルクや脱脂粉乳のようなラクトースを含む材料を用いることで、二糖類としてラクトースを付着液に添加する原料としても使用できる。 The milk protein is not particularly limited, and conventionally known materials containing various milk proteins can be used. It is possible to use a raw material having a high milk fat content such as skim milk powder, but when producing a viable bacterial preparation for fat decomposition such as used in a grease trap, it is preferable that the oil content in the preparation is low, so for example. , Preferred specific examples include casein, casein sodium, whey, concentrated whey, whey protein concentrate (WPC), whey protein isolate (WPI), whey powder, skim milk, milk protein concentrate (MPC), skim milk powder, α. -Lactalbumin, β-lactoglobulin and the like can be exemplified, and these can be used alone or in combination of two or more. By using a material containing lactose such as skim milk or skim milk powder as a milk protein raw material, it can also be used as a raw material for adding lactose as a disaccharide to an adhering liquid.
付着液中の乳タンパク質の含有量は、本発明の所望の効果が達成される限りにおいて、特に制限されない。製剤化工程における微生物の生存率の観点から、好ましくは、付着液が、乾燥菌体として1重量部の前記無芽胞菌に対して、0.01〜5重量部の乳タンパク質を含み、より好ましくは0.02〜1重量部の乳タンパク質を含み、更に好ましくは0.05〜0.5重量部の乳タンパク質を含む。 The content of milk protein in the adhering liquid is not particularly limited as long as the desired effect of the present invention is achieved. From the viewpoint of the viability of the microorganism in the formulation step, the adhering liquid preferably contains 0.01 to 5 parts by weight of milk protein with respect to 1 part by weight of the non-spore-forming bacteria as dried cells, more preferably. Contains 0.02 to 1 parts by weight of milk protein, more preferably 0.05 to 0.5 parts by weight of milk protein.
アミノ酸は、例えばグリシン、アラニン、バリン、ロイシン、イソロイシン、フェニルアラニン、グルタミン、グルタミン酸、アスパラギン、アスパラギン酸、システイン、リジン、セリン、スレオニン、メチオニン、トリプトファン、ヒスチジン、アルギン、プロリン、チロシン、およびこれらの誘導体が例示できる。付着液がアミノ酸を含むことにより、製剤化工程における微生物の生存率が向上する。アミノ酸の誘導体としては、アミノ酸の塩(例えば、カリウム塩、ナトリウム塩)、エステル体、水和物、溶媒和物等が例示できる。上記のアミノ酸は、1種を単独で、または2種以上を混合して用いることができる。上記のうち、製剤化工程における微生物の生存率の観点から、アミノ酸はグリシン、アラニン、およびこれらの誘導体からなる群から選択される1種以上であることが好ましく、グリシンおよびその誘導体から選択される1種以上であることが更に好ましい。 Amino acids include, for example, glycine, alanine, valine, leucine, isoleucine, phenylalanine, glutamine, glutamic acid, asparagine, aspartic acid, cysteine, lysine, serine, threonine, methionine, tryptophan, histidine, argin, proline, tyrosine, and derivatives thereof. It can be exemplified. Since the adhering liquid contains amino acids, the survival rate of microorganisms in the formulation process is improved. Examples of the amino acid derivative include amino acid salts (for example, potassium salt and sodium salt), esters, hydrates, solvates and the like. The above amino acids may be used alone or in admixture of two or more. Among the above, from the viewpoint of the survival rate of microorganisms in the formulation step, the amino acid is preferably one or more selected from the group consisting of glycine, alanine, and derivatives thereof, and is selected from glycine and its derivatives. It is more preferable that the number is one or more.
付着液中のアミノ酸の含有量は、本発明の所望の効果が達成される限りにおいて、特に制限されない。製剤化工程における微生物の生存率の観点から、好ましくは、付着液が、乾燥菌体として1重量部の前記無芽胞菌に対して、0.03〜10重量部のアミノ酸を含み、より好ましくは0.1〜5重量部のアミノ酸を含み、さらに好ましくは0.1〜3重量部のアミノ酸を含む。なお、付着液が2種以上のアミノ酸を含む場合、上記数値は、2種以上の合計量を示す。 The content of amino acids in the adhering liquid is not particularly limited as long as the desired effect of the present invention is achieved. From the viewpoint of the viability of the microorganism in the formulation step, the adhering liquid preferably contains 0.03 to 10 parts by weight of amino acids with respect to 1 part by weight of the non-spore-forming bacteria as dried cells, more preferably. It contains 0.1 to 5 parts by weight of amino acids, more preferably 0.1 to 3 parts by weight of amino acids. When the adhering liquid contains two or more kinds of amino acids, the above numerical value indicates the total amount of two or more kinds.
金属塩は、例えばナトリウム(Na)、カリウム(K)、マグネシウム(Mg)、カルシウム(Ca)、マンガン(Mn)、鉄(Fe)、ニッケル(Ni)、亜鉛(Zn)、銅(Cu)などの金属元素の、硫酸塩、亜硫酸塩、次亜硫酸塩、過硫酸塩、チオ硫酸塩、炭酸塩、リン酸塩、ピロリン酸、塩酸塩、硝酸塩、亜硝酸塩、酢酸塩、プロピオン酸塩、酪酸塩、クエン酸塩、シュウ酸塩、ハロゲン化物(たとえば、フッ化物、塩化物、臭化物、ヨウ化物)等が例示できるが、これらに限定されない。製剤化工程における付着液に金属塩を存在させることで、好適なトレハロースの含有量である0.8重量部以上1.5重量部未満としても、保存期間中における微生物の生存率の低下を防止し得る。金属塩は、より具体的には、例えば、硫酸ナトリウム、亜硫酸ナトリウム、次亜硫酸ナトリウム、チオ硫酸ナトリウム、炭酸ナトリウム、過硫酸ナトリウム、リン酸一ナトリウム、リン酸二ナトリウム、リン酸三ナトリウム、酢酸ナトリウム、硝酸ナトリウム、亜硝酸ナトリウム、酢酸ナトリウム、クエン酸ナトリウム、シュウ酸ナトリウム、塩化ナトリウム、硫酸カリウム、亜硫酸カリウム、次亜硫酸カリウム、チオ硫酸カリウム、炭酸カリウム、過硫酸カリウム、リン酸一カリウム、リン酸二カリウム、リン酸三カリウム、酢酸カリウム、硝酸カリウム、亜硝酸カリウム、酢酸カリウム、クエン酸カリウム、シュウ酸カリウム、塩化カリウム、硫酸マグネシウム、亜硫酸マグネシウム、チオ硫酸マグネシウム、炭酸マグネシウム、第一リン酸マグネシウム、第二リン酸マグネシウム、第三リン酸マグネシウム、ピロリン酸マグネシウム、硝酸マグネシウム、亜硝酸マグネシウム、酢酸マグネシウム、クエン酸マグネシウム、シュウ酸マグネシウム、塩化マグネシウム、硫酸カルシウム、亜硫酸カルシウム、チオ硫酸カルシウム、炭酸カルシウム、硝酸カルシウム、亜硝酸カルシウム、酢酸カルシウム、クエン酸カルシウム、シュウ酸カルシウム、塩化カルシウム等が例示できる。上記の金属塩は、1種を単独で、または2種以上を混合して用いることができる。 Examples of the metal salt include sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), nickel (Ni), zinc (Zn), copper (Cu) and the like. Metal elements of, sulfate, sulfite, hyposulfate, persulfate, thiosulfate, carbonate, phosphate, pyrophosphate, hydrochloride, nitrate, nitrite, acetate, propionate, butyrate , Citrate, oxalate, halides (eg, fluoride, chloride, bromide, iodide) and the like, but are not limited thereto. By allowing the metal salt to be present in the adhering liquid in the formulation step, even if the suitable trehalose content is 0.8 parts by weight or more and less than 1.5 parts by weight, the decrease in the survival rate of microorganisms during the storage period is prevented. Can be done. More specifically, the metal salt is, for example, sodium sulfate, sodium sulfite, sodium hyposulfate, sodium thiosulfate, sodium carbonate, sodium persulfate, monosodium phosphate, disodium phosphate, trisodium phosphate, sodium acetate. , Sodium nitrate, sodium nitrite, sodium acetate, sodium citrate, sodium oxalate, sodium chloride, potassium sulfate, potassium sulfite, potassium hyposulfate, potassium thiosulfate, potassium carbonate, potassium persulfate, monopotassium phosphate, phosphoric acid Dipotassium, tripotassium phosphate, potassium acetate, potassium nitrate, potassium nitrite, potassium acetate, potassium citrate, potassium oxalate, potassium chloride, magnesium sulfate, magnesium sulfite, magnesium thiosulfate, magnesium carbonate, magnesium monophosphate, first Magnesium diphosphate, magnesium tertiary phosphate, magnesium pyrophosphate, magnesium nitrate, magnesium nitrite, magnesium acetate, magnesium citrate, magnesium oxalate, magnesium chloride, calcium sulfate, calcium sulfite, calcium thiosulfate, calcium carbonate, nitrate Examples thereof include calcium, calcium nitrite, calcium acetate, calcium citrate, calcium oxalate, and calcium chloride. The above metal salts may be used alone or in admixture of two or more.
保存期間中における微生物の生存率が特に優れるという観点から、金属塩が、Na、K、MgおよびCaからなる群から選択される元素の、硫酸塩、炭酸塩、リン酸塩、またはそれらの組み合わせであることが好ましく、Mgおよび/またはCaの硫酸塩であることがより好ましい。なお、上記の「金属塩」には、金属塩の水和物や溶媒和物も含まれる。 Sulfates, carbonates, phosphates, or combinations thereof, in which the metal salt is an element selected from the group consisting of Na, K, Mg and Ca, from the viewpoint of particularly excellent microbial viability during the storage period. Is preferable, and it is more preferable that it is a sulfate of Mg and / or Ca. The above-mentioned "metal salt" also includes hydrates and solvates of metal salts.
付着液中の金属塩の含有量は、本発明の所望の効果が達成される限りにおいて、特に制限されない。保存期間中における微生物の生存率の観点から、好ましくは、付着液が、乾燥菌体として1重量部の前記無芽胞菌に対して、0.02〜10重量部の金属塩を含み、より好ましくは0.05〜1.5重量部の金属塩を含み、更に好ましくは0.1〜1重量部の金属塩を含む。なお、付着液が2種以上の金属塩を含む場合、上記数値は、2種以上の合計量を示す。 The content of the metal salt in the adhering liquid is not particularly limited as long as the desired effect of the present invention is achieved. From the viewpoint of the viability of the microorganism during the storage period, the adhering liquid preferably contains 0.02 to 10 parts by weight of a metal salt with respect to 1 part by weight of the non-spore-forming bacterium as a dry bacterial cell, more preferably. Contains 0.05 to 1.5 parts by weight of the metal salt, more preferably 0.1 to 1 part by weight of the metal salt. When the adhering liquid contains two or more kinds of metal salts, the above numerical value indicates the total amount of two or more kinds.
付着液には、上記の各成分のほか、本発明の目的効果が阻害されない範囲において、任意に、例えばグルコース、フルクトース等の単糖類;スクロース、ラクトース、マルトース等のトレハロース以外の二糖類;シクロデキストリン、ヒドロキシプロピルセルロース、ヒドロキシプロピルメチルセルロース、カルボキシメチルセルロース、結晶セルロース、コーンスターチ等の多糖類;大豆タンパク等のタンパク質;大豆ペプチド、ゼラチン、ペプトン、トリプトン等のタンパク加水分解物やペプチド;大豆油、菜種油、パーム油、ゴマ油、オリーブ油等の油脂;アスコルビン酸やその塩、トコフェロール等のビタミン類
;ポリグリセリン脂肪酸エステル、ショ糖脂肪酸エステル、ソルビタン脂肪酸エステル等の界面活性剤;ポリエチエレングリコール、グリセリンなどを添加してもよい。
In addition to the above components, the adhering solution may optionally contain monosaccharides such as glucose and fructose; disaccharides other than trehalose such as sucrose, lactose and maltose; cyclodextrin, as long as the intended effects of the present invention are not impaired. , Hydroxypropyl cellulose, hydroxypropylmethyl cellulose, carboxymethyl cellulose, crystalline cellulose, corn starch and other polysaccharides; soybean protein and other proteins; soybean peptides, gelatin, peptone, trypton and other protein hydrolysates and peptides; soybean oil, rapeseed oil, palm Fats and oils such as oil, sesame oil, olive oil; vitamins such as ascorbic acid and its salts, tocopherols; surfactants such as polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester; May be good.
付着液の固形分量は、多孔質担体への付着が可能であれば特に制限されないが、生産性等の観点から、付着液全体中の例えば3〜70重量%であり、好ましくは20〜50重量%である。付着液の調製方法も特に制限されず、上記した付着液に含まれる各成分を水に添加し、必要に応じて撹拌して調製する。 The solid content of the adhering liquid is not particularly limited as long as it can be adhered to the porous carrier, but from the viewpoint of productivity and the like, it is, for example, 3 to 70% by weight, preferably 20 to 50% by weight of the entire adhering liquid. %. The method for preparing the adhering liquid is also not particularly limited, and each component contained in the above-mentioned adhering liquid is added to water, and if necessary, the adhering liquid is prepared by stirring.
(3)多孔質担体
本実施形態では無芽胞菌の担体として多孔質担体を用い、これに直接無芽胞菌を付着させている。これにより、製剤化後及び保存中の含水率が微生物にとって最適な値となるため、微生物の生存率が向上するものと考えられる。
(3) Porous Carrier In the present embodiment, a porous carrier is used as a carrier for spore-free bacteria, and the spore-free bacteria are directly attached to the porous carrier. As a result, the water content after formulation and during storage becomes the optimum value for the microorganism, and it is considered that the survival rate of the microorganism is improved.
本発明においては、有機または無機の多孔質担体を広く利用することができる。多孔質担体に上記のような無芽胞菌を含む付着液を付着させると、多孔質担体を用いない場合や、多孔質以外の担体に付着させた場合と比較して、製剤化工程後の微生物の生存率を向上させることができる。ここで、付着させるとは、付着液を多孔質担体に噴霧する形態;付着液を多孔質担体に浸漬する形態などが挙げられる。好適には、無芽胞菌および水を含む噴霧液を、多孔質担体に噴霧する。 In the present invention, an organic or inorganic porous carrier can be widely used. When an adhering solution containing spore-free bacteria as described above is attached to the porous carrier, the microorganisms after the formulation step are compared with the case where the porous carrier is not used or when it is attached to a carrier other than the porous carrier. Survival rate can be improved. Here, the term “adherence” includes a form in which the adhering liquid is sprayed on the porous carrier; a form in which the adhering liquid is immersed in the porous carrier, and the like. Preferably, a spray solution containing spore-free bacteria and water is sprayed onto the porous carrier.
本発明において用いられうる多孔質担体の材料としては、特に制限されるものではないが、例えば、珪藻土、パーライト、バーミキュライト、タルク、クレー、ゼオライト、ベントナイト、カオリン、炭酸カルシウム、活性白土、二酸化チタン、珪砂、軽石、活性炭、カーボンブラック、グラファイト、ポリ乳酸、ポリスチレン、ポリ塩化ビニル等が例示できるが、これらに限定されない。製剤化工程後の微生物の生存率の観点から、多孔質担体としては、珪藻土、および活性炭からなる群から選択されることが好ましく、珪藻土であることがより好ましい。 The material of the porous carrier that can be used in the present invention is not particularly limited, but for example, diatomaceous earth, perlite, vermiculite, talc, clay, zeolite, bentonite, kaolin, calcium carbonate, activated clay, titanium dioxide, etc. Examples thereof include, but are not limited to, diatomaceous earth, pumice stone, activated carbon, carbon black, graphite, polylactic acid, polystyrene, and polyvinyl chloride. From the viewpoint of the survival rate of microorganisms after the formulation step, the porous carrier is preferably selected from the group consisting of diatomaceous earth and activated carbon, and more preferably diatomaceous earth.
なお、本明細書において「多孔質」とは、担体の表面に多数の小さな気泡状の空隙を有する状態をいう。好ましくは、多孔質担体の嵩密度(タッピング嵩密度)が0.01〜2g/mlである。上記のような嵩密度の多孔質担体を用いることにより、製剤化工程後の微生物の生存率をより一層向上できる。多孔質担体の嵩密度は、より好ましくは0.05〜1.5g/mlであり、更に好ましくは0.2〜1g/mlである。なお、上記の嵩密度は、タッピング嵩密度測定法により測定した値である。 In addition, in this specification, "porous" means a state which has a large number of small bubble-like voids on the surface of a carrier. Preferably, the bulk density (tapping bulk density) of the porous carrier is 0.01 to 2 g / ml. By using the bulky porous carrier as described above, the survival rate of microorganisms after the formulation step can be further improved. The bulk density of the porous carrier is more preferably 0.05 to 1.5 g / ml, still more preferably 0.2 to 1 g / ml. The above bulk density is a value measured by the tapping bulk density measuring method.
製剤化工程後の微生物の生存率の観点から、担体は、平均粒径(直径、体積基準)が1〜300μmの粒子状であることが好ましく、平均粒径(直径、体積基準)が10〜200μmの粒子状であることがより好ましく、30〜150μmであることが更に好ましい。なお、担体の粒径は、レーザー回折法にて測定した値である。 From the viewpoint of the viability of microorganisms after the formulation step, the carrier is preferably in the form of particles having an average particle size (diameter, volume standard) of 1 to 300 μm, and an average particle size (diameter, volume standard) of 10 to 10. It is more preferably in the form of particles of 200 μm, and further preferably in the form of particles of 30 to 150 μm. The particle size of the carrier is a value measured by a laser diffraction method.
上記の多孔質担体に対して、上述した無芽胞菌と場合により微生物保護剤とを含む付着液を付着させて製剤化する。 The above-mentioned porous carrier is formulated by adhering an adhering liquid containing the above-mentioned non-spore-forming bacteria and, in some cases, a microbial protective agent.
好適な形態である多孔質担体への噴霧液の噴霧は、流動層造粒機を用いて行うことが好ましい。すなわち、本発明の一実施形態では、生菌製剤の製剤化が流動層造粒法によって行われる。流動層造粒法により製剤化することにより、無芽胞菌と微生物保護剤とを含む無芽胞菌含有層を比較的低温で多孔質担体上に形成することができる。このため、製剤化工程における生存率の向上の観点から有利である。また、流動層造粒法により製剤化することにより、上記の無芽胞菌含有層を均一に多孔質担体上に形成することができる、という点においても有利である。 Spraying the spray liquid onto the porous carrier, which is a suitable form, is preferably performed using a fluidized bed granulator. That is, in one embodiment of the present invention, the live bacterial preparation is formulated by the fluidized bed granulation method. By formulating by the fluidized bed granulation method, a spore-free bacterium-containing layer containing spore-free bacteria and a microbial protective agent can be formed on the porous carrier at a relatively low temperature. Therefore, it is advantageous from the viewpoint of improving the survival rate in the formulation process. It is also advantageous in that the above-mentioned spore-free bacterium-containing layer can be uniformly formed on the porous carrier by formulating it by the fluidized bed granulation method.
多孔質担体に対する噴霧液の噴霧量は任意に設定でき、特に制限されるものではないが、1重量部の多孔質担体に対して、噴霧液の固形分として、例えば、0.1〜10重量部となる割合であり、好ましくは0.5〜5重量部となる割合である。 The amount of the spray liquid sprayed on the porous carrier can be arbitrarily set and is not particularly limited, but the solid content of the spray liquid is, for example, 0.1 to 10 weights based on 1 part by weight of the porous carrier. It is a ratio of parts, preferably 0.5 to 5 parts by weight.
噴霧液は、通常は50〜300g/分程度の速度で多孔質担体へ噴霧される。 The spray liquid is usually sprayed onto the porous carrier at a rate of about 50 to 300 g / min.
流動層造粒法により製剤化する場合は、温風の温度は微生物の耐熱性等を考慮して適宜設定すればよいが、例えば、入口温度30〜60℃であり、好ましくは35〜50℃である。流動層の温度も微生物の耐熱性等を考慮して適宜設定すればよいが、例えば、温度30〜60℃であり、好ましくは35〜50℃である。噴霧後、噴霧液を噴霧した多孔質担体を流動層造粒機内でそのまま乾燥してもよいし、別途乾燥機で乾燥してもよい。 When the product is formulated by the fluidized bed granulation method, the temperature of the warm air may be appropriately set in consideration of the heat resistance of microorganisms and the like. For example, the inlet temperature is 30 to 60 ° C, preferably 35 to 50 ° C. Is. The temperature of the fluidized bed may be appropriately set in consideration of the heat resistance of microorganisms and the like. For example, the temperature is 30 to 60 ° C, preferably 35 to 50 ° C. After spraying, the porous carrier sprayed with the spray liquid may be dried as it is in the fluidized bed granulator, or may be dried separately in the dryer.
粉末化した生菌製剤は、用途に応じて、公知のコーティング剤で表面の一部または全部を被覆してもよい。また、滑沢剤(例えば、タルク、マイカ、シリカ、ステアリン酸マグネシウム)、乾燥剤(酸化カルシウム、シリカゲル)、フィラーなどの粉末を、任意の割合で生菌製剤と混合し、混合製剤としてもよい。また、所望の粒度となるように製剤を粉砕したり、分級をしたりしてもよい。 The powdered live bacterial preparation may be partially or wholly coated with a known coating agent, depending on the intended use. Further, powders such as a lubricant (for example, talc, mica, silica, magnesium stearate), a desiccant (calcium oxide, silica gel), a filler and the like may be mixed with a viable bacterial preparation at an arbitrary ratio to form a mixed preparation. .. In addition, the formulation may be crushed or classified so as to have a desired particle size.
保存期間中の微生物の生存率の観点から、製剤化後の生菌製剤の水分含量は、3〜8重量%であることが好ましく、4.5〜7.5重量%であることがより好ましい。 From the viewpoint of the survival rate of microorganisms during the storage period, the water content of the live bacterial preparation after formulation is preferably 3 to 8% by weight, more preferably 4.5 to 7.5% by weight. ..
<排水処理方法>
本発明の第二の実施形態は、第一実施形態の製造方法で得られた生菌製剤を、油分を含む排水に接触させる工程を含み、生菌製剤中の無芽胞菌として、ヤロウィア・リポリティカ(Yarrowia lipolytica)を含む、排水処理方法に関する。ヤロウィア属の酵母は油分分解活性が高い油分分解性微生物であるため、排水処理用の生菌製剤に含まれる無芽胞菌として適している。以下、図1を参酌しながら、本側面に係る排水処理方法についてより詳細に説明する。なお、本発明の排水処理方法が、図1に限定されるものではない。
<Wastewater treatment method>
The second embodiment of the present invention includes a step of bringing the live bacterial preparation obtained by the production method of the first embodiment into contact with waste water containing oil, and as a spore-free bacterium in the live bacterial preparation, Yarrowia lipolytica. (Yarrowia lipolytica), including wastewater treatment methods. Since yeast of the genus Yarrowia is an oil-degradable microorganism having high oil-decomposing activity, it is suitable as a spore-free bacterium contained in a live bacterial preparation for wastewater treatment. Hereinafter, the wastewater treatment method according to this aspect will be described in more detail with reference to FIG. The wastewater treatment method of the present invention is not limited to FIG.
図1は、グリーストラップ10による排水処理(廃水処理)の仕組みを模式的に表している。本発明に係る方法において、上記の生菌製剤は、グリーストラップ10に排出する前の排水にあらかじめ添加されていても良いが、典型的には、排水処理槽1中の排水へ添加される。但し、本発明に係る排水処理方法は、上記生菌製剤と油分含有排水とを接触させることができる限り特に限定されない。
FIG. 1 schematically shows the mechanism of wastewater treatment (wastewater treatment) by the
グリーストラップ10は、埋設式、可動式など、設置形態は特に制限されない。埋設式の場合、例えば厨房や食品加工場において、排水路に流出した排水が残渣受け3に注ぎ込まれるように、グリーストラップ10を埋設する。可動式の場合、例えば、シンクの排水溝の下部に残渣受け3が位置するようにグリーストラップ10を設置する。
The installation form of the
図1において、排水は、矢印の方向へ流れる。なお、グリーストラップ10への排水の投入は、回分式であっても連続式であっても良い。油分含有排水は、残渣受け3を通じて排水処理槽1へと流れ込む。このとき、生ゴミ等の残渣の全部または一部は残渣受け3で捕集されるが、大部分の油分は残渣受け3を通過して排水処理槽1へと流入する。排水処理槽1へ流入した油分6は水面5へ向かって浮上し、仕切り板2aと2cとで仕切られた空間に集まる。従って、油分分解性微生物を含む生菌製剤を排水に加えない場合、仕切り板2aと2cとで仕切られた空間で油分6が次第に凝集し、スカムを形成することとなる。
In FIG. 1, the drainage flows in the direction of the arrow. The drainage into the
生菌製剤をグリーストラップ10に適用した場合、排水処理槽1にて(主として、仕切り板2aと2cとで仕切られた空間にて)、油分を含む排水と生菌製剤(油分分解性微生物)とが接触することとなる。生菌製剤に含まれるヤロウィア・リポリティカは油分の分解活性が高く、資化性を有するため、油分6の凝集を抑制し、スカムが形成されることを有効に防止し得る。特に、ヤロウィア・リポリティカ LM02−011株は、広範なpH領域(例えば、pH3.0〜11.0)においても高い油分分解活性を備える。これにより、排水のpHに依存せず、油分がトラップ管4を通じて外部環境へ流出することを防止し、環境保全の観点からも利点がある。
When the live bacterium preparation is applied to the
本実施形態に係る方法において使用される生菌製剤は、例えば、排水中へ添加され、排水と接触させられる。生菌製剤を容器に収容してグリーストラップ内に設置し、容器に排水を通液させることにより生菌製剤と排水とを接触させることもできる。これにより、排水と共に油分分解性微生物が流出して菌数が低下することを防止し得る。 The live bacterial preparation used in the method according to the present embodiment is added into, for example, wastewater and brought into contact with the wastewater. It is also possible to bring the live bacterial product into contact with the waste water by storing the live bacterial product in a container and installing it in a grease trap and allowing the drainage to pass through the container. As a result, it is possible to prevent the oil-decomposable microorganisms from flowing out together with the wastewater and the number of bacteria from decreasing.
本実施形態にかかる方法において、排水に生菌製剤を添加して接触させる場合、添加する菌量は任意に設定できる。排水に添加する生菌製剤の量は、特に制限されるものではないが、排水に含まれる油分1gに対して、製剤中の生菌数として例えば1×104〜1×1012CFUであり、好ましくは1×105〜1×1011CFUである。あるいは、排水に含まれる油分1gに対して、添加される生菌製剤の量は、例えば0.1mg〜100gであり、好ましくは1mg〜5gである。または、グリーストラップ内の排水に対して、製剤中の生菌数として例えば1×106〜1×1012CFU/L、より好ましくは1×107〜1×1011CFU/Lとなるような量を添加してもよい。あるいは、グリーストラップ内の排水に対して、添加される生菌製剤の量は、例えば10mg〜1000g/Lであり、好ましくは0.5g〜50g/Lである。 In the method according to the present embodiment, when the live bacterial preparation is added to the wastewater and brought into contact with the wastewater, the amount of the bacterial to be added can be arbitrarily set. The amount of the viable bacterial preparation to be added to the wastewater is not particularly limited, but the viable cell count in the preparation is, for example, 1 × 10 4 to 1 × 10 12 CFU per 1 g of oil contained in the wastewater. , Preferably 1 × 10 5 to 1 × 10 11 CFU. Alternatively, the amount of the live bacterial preparation added with respect to 1 g of the oil contained in the waste water is, for example, 0.1 mg to 100 g, preferably 1 mg to 5 g. Alternatively, the viable cell count in the formulation is, for example, 1 × 10 6 to 1 × 10 12 CFU / L, more preferably 1 × 10 7 to 1 × 10 11 CFU / L with respect to the waste water in the grease trap. May be added in an amount. Alternatively, the amount of the live bacterial preparation added with respect to the waste water in the grease trap is, for example, 10 mg to 1000 g / L, preferably 0.5 g to 50 g / L.
排水を外部環境へ排出する際、生菌製剤が排水と共にグリーストラップ外へと排出される場合があるので、本実施形態においては、グリーストラップ(排水)に、定期的に生菌製剤を添加してもよい。添加する間隔は特に制限されないが、例えば、1回/3時間、1回/24時間、または2〜3日に1回の間隔で添加するのが好ましい。添加する方法は特に制限されず、排水が連続的にグリーストラップに流入する場合には、排水に混在させて添加してもよいし、グリーストラップ内の排水に直接、添加してもよい。厨房のシンクなどの排水口から生菌製剤を添加すれば、洗浄により排出される排水とともに、生菌製剤をグリーストラップ内に導入することができる。 When the wastewater is discharged to the external environment, the live bacterial preparation may be discharged to the outside of the grease trap together with the wastewater. Therefore, in the present embodiment, the live bacterial preparation is periodically added to the grease trap (drainage). You may. The interval of addition is not particularly limited, but it is preferable to add at intervals of, for example, once / three hours, once / 24 hours, or once every 2 to 3 days. The method of addition is not particularly limited, and when the wastewater continuously flows into the grease trap, it may be added mixed with the wastewater or may be added directly to the wastewater in the grease trap. If the live-bacteria preparation is added from a drain outlet such as a kitchen sink, the live-bacteria preparation can be introduced into the grease trap together with the wastewater discharged by washing.
本明細書において「油分」とは、トリグリセリド、ジグリセリドおよびモノグリセリドのようなアシルグリセロールを50重量%以上含む(例えば、65重量%以上、好ましくは80重量%以上、より好ましくは90重量%以上(上限100重量%)のグリセリド類を含む)食用または工業用油脂、ならびに脂肪酸を指す。油脂は、ステロール、リン脂質等のアシルグリセロール以外の脂質を含むものであっても良い。 As used herein, the term "oil" contains 50% by weight or more of acylglycerol such as triglyceride, diglyceride and monoglyceride (for example, 65% by weight or more, preferably 80% by weight or more, more preferably 90% by weight or more (upper limit). Refers to edible or industrial fats and oils (including 100% by weight) of glycerides, as well as fatty acids. The fats and oils may contain lipids other than acylglycerol such as sterols and phospholipids.
油脂としては、各種の動植物性油脂が含まれ、例えば、オリーブ油、キャノーラ油、ココナッツ油、ごま油、米油、米ぬか油、サフラワー油、大豆油、トウモロコシ油、菜種油、パーム油、パーム核油、ひまわり油、綿実油、やし油、落花生油、カカオ脂、牛脂、ラード、鶏油、魚油、鯨油、バター、マーガリン、ファットスプレッド、ショートニング等の食用油脂;およびアマニ油、ジャトロファ油、トール油、ハマナ油、ひまし油、ホホバ油等の工業用油脂;が例示できるが、好ましくはグリーストラップが設置されることが多いレストラン等で頻繁に排出される食用油脂である。 Oils and fats include various animal and vegetable oils and fats, for example, olive oil, canola oil, coconut oil, sesame oil, rice oil, rice bran oil, saflower oil, soybean oil, corn oil, rapeseed oil, palm oil, palm kernel oil, etc. Sunflower oil, cottonseed oil, palm oil, peanut oil, cacao butter, beef oil, lard, chicken oil, fish oil, whale oil, butter, margarine, fat spread, shortening and other edible oils; Industrial fats and oils such as oil, castor oil, and jojoba oil; can be exemplified, but preferably edible fats and oils that are frequently discharged in restaurants and the like where grease traps are often installed.
脂肪酸としては、特に限定されるものではないが、例えば、酪酸、ヘキサン酸、ヘプタ
ン酸、オクタン酸、デカン酸、ラウリン酸、トリデカン酸、ミリスチン酸、ペンタデカン酸、ペンタデカン酸、パルミチン酸、ヘプタデカン酸、ステアリン酸、アラキジン酸、ベヘン酸、リグノセリン酸等の飽和脂肪酸;デセン酸、ミリストレイン酸、ペンタデセン酸、パルミトレイン酸、ヘプタデセン酸、オレイン酸、イコセン酸、ドコセン酸、テトラコセン酸、ヘキサデカジエン酸、ヘキサデカトリエン酸、ヘキサデカテトラエン酸、リノール酸、α−リノレン酸、γ−リノレン酸、オクタデカテトラエン酸、イコサジエン酸、イコサトリエン酸、イコサテトラエン酸、アラキドン酸、イコサペンタエン酸、ヘンイコサペンタエン酸、ドコサジエン酸、ドコサテトラエン酸、ドコサペンタエン酸、ドコサペンタエン酸、ドコサヘキサエン酸等の不飽和脂肪酸;が挙げられる。脂肪酸は、食用または工業用油脂が分解されて生じたものであっても良い。
The fatty acid is not particularly limited, but for example, butyric acid, hexanoic acid, heptanic acid, octanoic acid, decanoic acid, lauric acid, tridecanoic acid, myristic acid, pentadecanoic acid, pentadecanoic acid, palmitic acid, heptadecanoic acid, Saturated fatty acids such as stearic acid, arachidic acid, bechenic acid, lignoseric acid; decenoic acid, myristoleic acid, pentadecenoic acid, palmitreic acid, heptadecenoic acid, oleic acid, icosenoic acid, docosenoic acid, tetracosenoic acid, hexadecadienic acid, hexa Decatrienoic acid, hexadecatetetraenoic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, octadecatetetraenoic acid, icosadienoic acid, icosatrienoic acid, icosatetraenoic acid, arachidonic acid, icosapentaenoic acid, henicosapentaenoic acid, docosadienoic acid , Docosatetraenoic acid, docosapentaenoic acid, docosapentaenoic acid, unsaturated fatty acids such as docosahexaenoic acid; The fatty acid may be produced by decomposing edible or industrial fats and oils.
排水中の油分の含有量は、特に制限されない。排水中に含まれる油分は、2種類以上であっても良い。 The content of oil in the wastewater is not particularly limited. The amount of oil contained in the wastewater may be two or more.
本実施形態の方法において、第一実施形態の製造方法で得られる生菌製剤に加えて、油分をより効率的に減少させる観点から、他の成分を排水に添加してもよい。他の成分としては、例えば、リパーゼやホスホリパーゼ等の油分分解性酵素、pH調整剤などが挙げられる。 In the method of the present embodiment, in addition to the live bacterial preparation obtained by the production method of the first embodiment, other components may be added to the waste water from the viewpoint of reducing the oil content more efficiently. Examples of other components include oil-degrading enzymes such as lipase and phospholipase, and pH adjusters.
油分分解性酵素としては、たとえば、シュードモナス(Pseudomonas)属、アスペルギルス(Aspergillus)属、バチルス(Bacillus)属、ペニシリウム(Penicillium)属、リゾプス(Rhizopus)属、リゾムコール(Rhizomucor)属、ムコール(Mucor)属、ペシロマイセス(Paecilomyces)属、リゾクトニア(Rhizoctonia)属、アブシディア(Absidia)属、アクロモバクター(Achromobacter)属、エロモナス(Aeromonas)属、アルテルナリア(Alternaria)属、アウレオバシジウム(Aureobasidium)属、ボーベリア(Beauveria)属、クロモバクター(Chromobacter)属、コプリヌス(Coprinus)属、フザリウム(Fusarium)属、ゲオトリクム(Geotricum)属、フミコラ(Humicola)属、ハイホジーマ(Hyphozyma)属、ラクトバチルス(Lactobacillus)属、メタリジウム(Metarhizium)属、ロドスポリジウム(Rhodosporidium)属、トリコデルマ(Trichoderma)属、ヤロウィア(Yarrowia)属、キャンディダ(Candida)属、ピキア(Pichia)属、ハンセヌラ(Hansenula)属、サッカロマイセス(Saccharomyces)属、クルイベロマイセス(Kluyveromyces)属、および/またはトリコスポロン(Trichosporon)属から得ることができる。 Examples of oil-degrading enzymes include the genus Pseudomonas, the genus Aspergillus, the genus Bacillus, the genus Penicillium, the genus Rhizopus, and the genus Rhizomu. , Pecilomyces, Rhizoctonia, Absidia, Achromobacter, Aeromonas, Alternaria, Alternaria, Auternaria Beauveria, Chromobacter, Coprinus, Fusarium, Geotricum, Humicola, Hyphozyl, Hyphozyl, Hyphozium Genus Metalhizium, genus Rhodospolidium, genus Trichoderma, genus Yarrowia, genus Candida, genus Pichia, genus Pichia, genus Hansenula, genus Hansenula, genus Hansenula It can be obtained from the genus Kluyveromyces and / or the genus Trichosporon.
市販の油分分解性酵素としては、リパーゼMY、リパーゼOF、リパーゼPL、リパーゼQLM(名糖産業株式会社);リパーゼA「アマノ(登録商標)」6、リパーゼDF「アマノ(登録商標)」15、リパーゼG「アマノ(登録商標)」50、リパーゼAY「アマノ(登録商標)」30SD、リパーゼR「アマノ(登録商標)」、リパーゼMER「アマノ(登録商標)」、ニューラーゼ(登録商標)F(アマノエンザイム株式会社);スミチーム(登録商標)NLS、スミチーム(登録商標)RLS(新日本化学工業株式会社);リリパーゼ(登録商標)A−10D、リリパーゼ(登録商標)AF−5、PLA2ナガセ(ナガセケムテックス株式会社);エンチロンAKG−2000、エンチロンLP、エンチロンLPG(洛東化成工業株式会社);Lipolase(登録商標)100T、Lipolase(登録商標)100L、Palatase20000L、Lipex(登録商標)100T、Lipex(登録商標)100L、Lipozyme(登録商標)RMIM、Lipozyme(登録商標)TLIM、Novozyme(登録商標)435FG(ノボザイムズ社製);ピカンターゼA、ピカンターゼR800(ディー・エス・エ
ムジャパン社製)等が挙げられる。これらを2種以上組み合わせて用いることもできる。
Commercially available oil-degrading enzymes include Lipase MY, Lipase OF, Lipase PL, Lipase QLM (Meishu Sangyo Co., Ltd.); Lipase A "Amano (registered trademark)" 6, Lipase DF "Amano (registered trademark)" 15, Lipase G "Amano (registered trademark)" 50, Lipase AY "Amano (registered trademark)" 30SD, Lipase R "Amano (registered trademark)", Lipase MER "Amano (registered trademark)", Neurose (registered trademark) F ( Amano Enzyme Co., Ltd.); Sumiteam (registered trademark) NLS, Sumiteam (registered trademark) RLS (Shin Nihon Kagaku Kogyo Co., Ltd.); Lilipase (registered trademark) A-10D, Lilipase (registered trademark) AF-5, PLA2 Nagase (Nagase) Chemtex Co., Ltd.); Entilon AKG-2000, Entilon LP, Entilon LPG (Rakuto Kasei Kogyo Co., Ltd.); Examples thereof include 100L of registered trademark, Lipozyme (registered trademark) RMIM, Lipozyme (registered trademark) TLIM, Novozime (registered trademark) 435FG (manufactured by Novozymes); Picantase A, Picantase R800 (manufactured by DSM Japan), and the like. .. It is also possible to use two or more of these in combination.
油分分解性酵素の量は、酵素が油分と反応できれば特に制限されないが、排水に含まれる油分1gに対して、10〜2,000Uで用いることが好ましい。より好ましくは50〜1,500U、さらに好ましくは100〜1,000Uである。または、グリーストラップの容量に対して、好ましくは1,000〜100,000U/L、より好ましくは2,000〜80,000U/Lとなるような量であってもよい。なお、油分分解性酵素の活性単位(U)は、37℃、pH7の条件で1分間に1μモルの脂肪酸を遊離する酵素量である。
The amount of the oil-degrading enzyme is not particularly limited as long as the enzyme can react with the oil, but it is preferably used at 10 to 2,000 U with respect to 1 g of the oil contained in the waste water. It is more preferably 50 to 1,500 U, still more preferably 100 to 1,000 U. Alternatively, the amount may be preferably 1,000 to 100,000 U / L, more preferably 2,000 to 80,000 U / L with respect to the capacity of the grease trap. The active unit (U) of the oil-degrading enzyme is the amount of the enzyme that liberates 1 μmol of fatty acid per minute under the conditions of 37 ° C. and
グリーストラップは、油分含有排水を連続的に導入し、処理後の排水を連続的に排出する形態であってもよいし、油分含有排水を導入し、一括して処理した後に、処理後の排水を一括して排出する形態であってもよい。 The grease trap may be in the form of continuously introducing the oil-containing wastewater and continuously discharging the treated wastewater, or after introducing the oil-containing wastewater and collectively treating the wastewater, the treated wastewater. May be in the form of discharging all at once.
また、本実施形態の方法において、生菌製剤と油分とを接触させる際の温度、すなわちグリーストラップ内の排水の温度としては、任意に設定することができる。また、生菌製剤と油分とを接触させる際のpH、すなわちグリーストラップ内の排水のpHとしても、任意に設定することができる。一般的には、温度は、例えば10〜60℃であり、20〜50℃が好ましく、25〜40℃がより好ましい。pHは例えば3〜10であり、pH4〜9が好ましく、pH5〜8がさらに好ましい。さらに、必要に応じて、排水基準を満たす範囲で、曝気等により排水にエアレーションを行っても良い。
Further, in the method of the present embodiment, the temperature at which the viable bacterial preparation and the oil component are brought into contact with each other, that is, the temperature of the drainage in the grease trap can be arbitrarily set. Further, the pH at the time of contacting the viable bacterial preparation with the oil content, that is, the pH of the waste water in the grease trap can be arbitrarily set. Generally, the temperature is, for example, 10 to 60 ° C, preferably 20 to 50 ° C, more preferably 25 to 40 ° C. The pH is, for example, 3 to 10, preferably
<生菌製剤の保存安定方法>
本発明の第三実施形態は、無芽胞菌および水を含む付着液を多孔質担体に付着させてなる生菌製剤の保存安定性向上方法であって、無芽胞菌を炭素数14〜18のモノ不飽和脂肪酸を含む培地で培養して得る、生菌製剤の保存安定性の向上方法である。無芽胞菌を炭素数14〜18のモノ不飽和脂肪酸を含む培地で培養することは、第一実施形態について説明したものと同様である。
<Preservation and stabilization method of live bacterial product>
The third embodiment of the present invention is a method for improving the storage stability of a live bacterial preparation obtained by adhering an adherent liquid containing spore-free bacteria and water to a porous carrier, wherein the spore-free bacterium has 14 to 18 carbon atoms. This is a method for improving the storage stability of a live bacterial preparation obtained by culturing in a medium containing a monounsaturated fatty acid. Culturing the spore-free bacteria in a medium containing monounsaturated fatty acids having 14 to 18 carbon atoms is similar to that described in the first embodiment.
炭素数14〜18のモノ不飽和脂肪酸を含む培地で培養した無芽胞菌を多孔質担体に付着させてなる生菌製剤により、40℃または50℃での保存安定性が顕著に向上する。例えば、製造直後の生菌数を100%とした場合、生菌製剤を50℃、7日間保存後の生菌率が50%以上、好ましくは80%以上となる。なお、ここでの生菌率は下記実施例に記載の方法により得られた値を採用する。 The viable bacterial preparation obtained by adhering a non-spore-forming bacterium cultured in a medium containing a monounsaturated fatty acid having 14 to 18 carbon atoms to a porous carrier significantly improves storage stability at 40 ° C. or 50 ° C. For example, assuming that the viable cell count immediately after production is 100%, the viable cell ratio after storing the viable bacterial preparation at 50 ° C. for 7 days is 50% or more, preferably 80% or more. As the viable cell rate here, the value obtained by the method described in the following Examples is adopted.
本発明の効果を、以下の実施例および比較例を用いて説明する。ただし、本発明の技術的範囲が以下の実施例のみに制限されるわけではない。 The effects of the present invention will be described with reference to the following examples and comparative examples. However, the technical scope of the present invention is not limited to the following examples.
(実施例1)
(1)液体培地の調製
終濃度が1.1重量%グルコース、0.3重量%硝酸ナトリウム、0.3重量%酵母エキス、0.4重量%麦芽エキス、0.002重量%オレイン酸(20μg/mL)、0.03重量%消泡剤(アデカネートB−317F:ADEKA社製)となるように純水に溶解したものを液体培地とした(培地pH4.7)。
(Example 1)
(1) Preparation of liquid medium The final concentration is 1.1% by weight glucose, 0.3% by weight sodium nitrate, 0.3% by weight yeast extract, 0.4% by weight malt extract, 0.002% by weight oleic acid (20 μg). / ML), 0.03 wt% antifoaming agent (Adecanate B-317F: manufactured by ADEKA) was dissolved in pure water to prepare a liquid medium (medium pH 4.7).
(2)種培養
坂口フラスコに上記の培地を各100mL入れ、オートクレーブ滅菌した。ヤロウィア・リポリティカ(Yarrowia lipolytica)LM02−011株(平成
26年3月6日付で、独立行政法人製品評価技術基盤機構 特許微生物寄託センター(千葉県木更津市かずさ鎌足2−5−8)に寄託されており、その受託番号は、NITE P−01813である。)を白金耳を用いて液体培地に接種して、ジャーファーメンターにて30℃で48時間(撹拌速度:300rpm)培養し、培養液を得た。
(2)
(3)本培養
1Lビーカーに液体培地を0.5L入れ、滅菌した。種培養を行った培養液100μLを液体培地に添加し、培養した(温度:30℃、通気量:1.6vvm、攪拌速度:140rpm、培養時間72h)。
(3) Main culture 0.5 L of liquid medium was placed in a 1 L beaker and sterilized. 100 μL of the culture solution obtained by seed culture was added to a liquid medium and cultured (temperature: 30 ° C., aeration rate: 1.6 vvm, stirring speed: 140 rpm, culture time 72 h).
(4)懸濁液の調製
得られた培養液を10分間遠心分離(×10,000g)し、菌体を回収した。得られた菌体に対して下記表1の比率で各成分を蒸留水に添加し、混合して噴霧液を調製した。なお、乳タンパク質としては、固形分中にタンパク質を35重量%、乳糖を52重量%の割合で含むスキムミルクを用いた。
(4) Preparation of suspension The obtained culture solution was centrifuged (× 10,000 g) for 10 minutes, and the cells were collected. Each component was added to distilled water at the ratio shown in Table 1 below with respect to the obtained bacterial cells, and mixed to prepare a spray solution. As the milk protein, skim milk containing 35% by weight of protein and 52% by weight of lactose in the solid content was used.
流動層造粒機(FA−LAB−1、株式会社パウレック社)に300gの珪藻土(平均粒径75μm、嵩密度0.33g/ml、ラヂオライト(登録商標)♯3000、昭和化学工業株式会社)をセットした。珪藻土に対して、1077gの上記噴霧液を143g/分の速度で噴霧するとともに、40℃の温風を送風して内容物を流動させた状態で乾燥させた。噴霧後、引き続いて流動層造粒機内で40℃の温風を40分間送風して、造粒物を乾燥させた。これにより、生菌製剤を得た。生菌製剤の水分含量は5.5重量%であった。 300 g of diatomaceous earth (average particle size 75 μm, bulk density 0.33 g / ml, radiolite (registered trademark) # 3000, Showa Kagaku Kogyo Co., Ltd.) in a fluidized bed granulator (FA-LAB-1, Paulec Co., Ltd.) Was set. 1077 g of the above spray liquid was sprayed on the diatomaceous earth at a rate of 143 g / min, and warm air at 40 ° C. was blown to dry the contents in a flowing state. After spraying, warm air at 40 ° C. was subsequently blown in the fluidized bed granulator for 40 minutes to dry the granulated product. As a result, a live bacterial preparation was obtained. The water content of the live bacterial preparation was 5.5% by weight.
(比較例1)
下記液体培地を用いたこと以外は、実施例1と同様にして生菌製剤を得た。
(Comparative Example 1)
A live bacterial preparation was obtained in the same manner as in Example 1 except that the following liquid medium was used.
(1)液体培地の調製
終濃度が1.1重量%グルコース、0.3重量%硝酸ナトリウム、0.3重量%酵母エキス、0.4重量%麦芽エキス、0.03重量%消泡剤(アデカネート(登録商標)B−317F:ADEKA社製)となるように純水に溶解したものを液体培地とした(培地pH4.7)。
(1) Preparation of liquid medium The final concentration is 1.1% by weight glucose, 0.3% by weight sodium nitrate, 0.3% by weight yeast extract, 0.4% by weight malt extract, 0.03% by weight antifoaming agent ( A liquid medium was prepared by dissolving it in pure water so as to be adecaneate (registered trademark) B-317F: manufactured by ADEKA (medium pH 4.7).
(比較例2)
下記液体培地を用いたこと以外は、実施例1と同様にして生菌製剤を得た。
(Comparative Example 2)
A live bacterial preparation was obtained in the same manner as in Example 1 except that the following liquid medium was used.
(1)液体培地の調製
終濃度が1.1重量%グルコース、0.3重量%硝酸ナトリウム、0.3重量%酵母エキス、0.4重量%麦芽エキス、0.02重量%ステアリン酸(20μg/L)、0.03重量%消泡剤(アデカネートB−317F:ADEKA社製)となるように純水に溶解したものを液体培地とした(培地pH4.7)。
(1) Preparation of liquid medium The final concentration is 1.1% by weight glucose, 0.3% by weight sodium nitrate, 0.3% by weight yeast extract, 0.4% by weight malt extract, 0.02% by weight stearic acid (20 μg). / L), 0.03% by weight antifoaming agent (Adecanate B-317F: manufactured by ADEKA) was dissolved in pure water to prepare a liquid medium (medium pH 4.7).
(評価:高温での保存安定性)
作製後の生菌製剤を、遮光30mLガラスバイアル瓶内に封入し、40℃および50℃の恒温槽に静置した。保存から3日後、5日後、および7日後における生菌製剤1g当たりの生菌数を以下の手法により求めた。
(Evaluation: Storage stability at high temperature)
The prepared live bacterial preparation was sealed in a light-shielded 30 mL glass vial and allowed to stand in a constant temperature bath at 40 ° C. and 50 ° C. The viable cell count per 1 g of the viable bacterial preparation at 3 days, 5 days, and 7 days after storage was determined by the following method.
実施例、比較例において製剤化直後(温風乾燥から60分以内)における生菌製剤中の生菌数を求めた。すなわち、生菌製剤を、100倍量の蒸留水中(25℃)で1分間撹拌して段階希釈し、得られた希釈液を寒天培地(組成:YM培地に、終濃度2重量%となるように寒天を添加したもの)の表面に塗布した。菌を30℃で48時間培養した後、寒天培地上に形成されたコロニー数を計測した。コロニー数から、製剤1g当たりの生菌数を算出した。なお、YM培地の組成は、0.3重量%酵母エキス、0.4重量%麦芽エキス、0.5重量%ペプトン、1.0重量%グルコース(各濃度は終濃度)である。 In Examples and Comparative Examples, the number of viable bacteria in the viable bacterial preparation immediately after formulation (within 60 minutes from warm air drying) was determined. That is, the viable cell preparation is serially diluted by stirring in 100 times the amount of distilled water (25 ° C.) for 1 minute, and the obtained diluted solution is added to an agar medium (composition: YM medium to a final concentration of 2% by weight). Was applied to the surface of (added agar). After culturing the bacteria at 30 ° C. for 48 hours, the number of colonies formed on the agar medium was counted. From the number of colonies, the number of viable bacteria per 1 g of the pharmaceutical product was calculated. The composition of the YM medium is 0.3% by weight yeast extract, 0.4% by weight malt extract, 0.5% by weight peptone, and 1.0% by weight glucose (each concentration is the final concentration).
各サンプルを40℃、および50℃に保存し、3日後、5日後および7日後について同様にコロニー数から、製剤1g当たりの生菌数を算出した。 Each sample was stored at 40 ° C. and 50 ° C., and the viable cell count per 1 g of the pharmaceutical product was calculated from the number of colonies in the same manner after 3 days, 5 days and 7 days.
製剤化直後の生菌数100%に対する各状態での生菌数を表2および図2に示す。 Table 2 and FIG. 2 show the viable cell counts in each state with respect to 100% viable cell count immediately after formulation.
図2に示すように、実施例で得られた生菌製剤は、製剤化工程における生菌率に優れていた。また、図2に示すように、実施例で得られた生菌製剤は40℃、または50℃での高温保存安定性に優れるものであった。特に50℃という高温環境下であっても7日後に50%以上が生存していたことは顕著に保存安定性に優れることを示す。一方、オレイン酸を含まない比較例1、オレイン酸の代わりに飽和脂肪酸であるステアリン酸を用いた比較例2は、40℃での保存安定性が5日目には両者とも80%未満であり、また50℃での保存安定性は、5日目には50%以下、7日目には20%未満となった。したがって、培地中にオレイン酸を添加することで、高温保存下での保存安定性が顕著に向上することが示された。 As shown in FIG. 2, the viable bacterial preparation obtained in the example was excellent in the viable cell ratio in the formulation step. Further, as shown in FIG. 2, the viable bacterial preparation obtained in the examples was excellent in high temperature storage stability at 40 ° C. or 50 ° C. In particular, the fact that 50% or more survived after 7 days even in a high temperature environment of 50 ° C. indicates that the storage stability is remarkably excellent. On the other hand, in Comparative Example 1 containing no oleic acid and Comparative Example 2 in which stearic acid, which is a saturated fatty acid, was used instead of oleic acid, the storage stability at 40 ° C. was less than 80% on the 5th day. The storage stability at 50 ° C. was 50% or less on the 5th day and less than 20% on the 7th day. Therefore, it was shown that the addition of oleic acid in the medium significantly improved the storage stability under high temperature storage.
<オレイン酸濃度の違いによる評価>
(実施例2)
実施例1の液体培地の調製において、オレイン酸濃度を20μg/mLから10μg/mLに変更したこと以外は、実施例1と同様にして液体培地を得た。
<Evaluation based on the difference in oleic acid concentration>
(Example 2)
A liquid medium was obtained in the same manner as in Example 1 except that the oleic acid concentration was changed from 20 μg / mL to 10 μg / mL in the preparation of the liquid medium of Example 1.
得られた液体培地を用いたこと以外は、実施例1と同様にして生菌製剤を得た。 A live bacterial preparation was obtained in the same manner as in Example 1 except that the obtained liquid medium was used.
(実施例3)
実施例1の液体培地の調製において、オレイン酸濃度を20μg/mLから40μg/mLに変更したこと以外は、実施例1と同様にして液体培地を得た。
(Example 3)
A liquid medium was obtained in the same manner as in Example 1 except that the oleic acid concentration was changed from 20 μg / mL to 40 μg / mL in the preparation of the liquid medium of Example 1.
得られた液体培地を用いたこと以外は、実施例1と同様にして生菌製剤を得た。 A live bacterial preparation was obtained in the same manner as in Example 1 except that the obtained liquid medium was used.
(実施例4)
実施例1の液体培地の調製において、オレイン酸濃度を20μg/mLから80μg/mLに変更したこと以外は、実施例1と同様にして液体培地を得た。
(Example 4)
A liquid medium was obtained in the same manner as in Example 1 except that the oleic acid concentration was changed from 20 μg / mL to 80 μg / mL in the preparation of the liquid medium of Example 1.
得られた液体培地を用いたこと以外は、実施例1と同様にして生菌製剤を得た。 A live bacterial preparation was obtained in the same manner as in Example 1 except that the obtained liquid medium was used.
上記実施例2〜4で得られた生菌製剤について、上記実施例1と同様にして50℃での保存安定性を評価した。結果を下記表3に示す。 The live bacterial preparations obtained in Examples 2 to 4 were evaluated for storage stability at 50 ° C. in the same manner as in Example 1. The results are shown in Table 3 below.
上記結果より、いずれの実施例も、50℃という高温環境下であっても5日後に75%以上、7日後に50%以上が生存していたことから、高温での保存安定性に優れることが示された。特に添加濃度が20μg/mL以上になると、50℃、7日後の生存率が80%以上と、顕著に高くなり、高温での保存安定性に一層優れることが示された。 From the above results, all of the examples were excellent in storage stability at high temperature because 75% or more survived after 5 days and 50% or more survived after 7 days even in a high temperature environment of 50 ° C. It has been shown. In particular, when the addition concentration was 20 μg / mL or more, the survival rate after 7 days at 50 ° C. was remarkably high at 80% or more, indicating that the storage stability at high temperature was further excellent.
本発明に係る生菌製剤の製造方法を利用することで、環境、食品、医薬品、農薬、公衆衛生等の分野において利用可能な生菌製剤を高い生存率で提供できる。好ましくは、本発明に係る生菌製剤の製造方法は、水質環境改善用生菌製剤、特に排水処理用生菌製剤の製造において好適に利用され得る。 By utilizing the method for producing a live bacterial preparation according to the present invention, it is possible to provide a live bacterial preparation that can be used in the fields of environment, food, pharmaceuticals, pesticides, public health, etc. with a high survival rate. Preferably, the method for producing a live bacterial preparation according to the present invention can be suitably used in the production of a live bacterial preparation for improving the water quality environment, particularly a live bacterial preparation for wastewater treatment.
1 排水処理槽、
2a、2b、2c 仕切り板、
3 残渣受け、
4 トラップ管、
5 水面、
6 油分、
10 グリーストラップ。
1 Wastewater treatment tank,
2a, 2b, 2c dividers,
3 Residue receiving,
4 Trap tube,
5 water surface,
6 oil,
10 Grease trap.
Claims (10)
前記ヤロウィア・リポリティカ(Yarrowia lipolytica)をオレイン酸を含む培地で培養して得る、生菌製剤の保存安定性の向上方法。 A method for improving the storage stability of a viable bacterial preparation, which is formed by adhering an adhering liquid containing Yarrowia lipolytica and water to a porous carrier.
A method for improving the storage stability of a live bacterial preparation, which is obtained by culturing the Yarrowia lipolytica in a medium containing oleic acid.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2017070752A JP6920089B2 (en) | 2017-03-31 | 2017-03-31 | Production method of live bacterial product, live bacterial product and wastewater treatment method using it |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2017070752A JP6920089B2 (en) | 2017-03-31 | 2017-03-31 | Production method of live bacterial product, live bacterial product and wastewater treatment method using it |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2018170988A JP2018170988A (en) | 2018-11-08 |
JP6920089B2 true JP6920089B2 (en) | 2021-08-18 |
Family
ID=64106442
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2017070752A Active JP6920089B2 (en) | 2017-03-31 | 2017-03-31 | Production method of live bacterial product, live bacterial product and wastewater treatment method using it |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP6920089B2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113773994A (en) * | 2021-09-23 | 2021-12-10 | 江苏蓝必盛化工环保股份有限公司 | Efficient hydrolytic acidification microbial agent, preparation method and application |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE59605660D1 (en) * | 1995-12-19 | 2000-08-31 | Biorem Ag Baar | SYSTEM FOR THE REMOVAL OF FATS AND OILS |
DE29724816U1 (en) * | 1997-01-09 | 2004-07-22 | Société des Produits Nestlé S.A. | Cereal product containing probiotics |
JP4161395B2 (en) * | 1998-01-16 | 2008-10-08 | クミアイ化学工業株式会社 | Fusarium spp. |
JPH11290078A (en) * | 1998-04-09 | 1999-10-26 | Kansai Kagaku Kikai Seisaku Kk | Yeast having lipase on cell surface and its utilization |
AU1417901A (en) * | 1999-11-26 | 2001-06-04 | Kansai Chemical Engineering Co., Ltd. | Process for producing fatty acid lower alcohol ester |
JP3824312B2 (en) * | 2000-05-02 | 2006-09-20 | ビオフェルミン製薬株式会社 | Dried bacterial cells by spray drying |
JP2004345999A (en) * | 2003-05-21 | 2004-12-09 | Taiko Pharmaceutical Co Ltd | Bacterial cell preparation composition |
JP2005325077A (en) * | 2004-05-17 | 2005-11-24 | Mitsui Chemicals Inc | Pseudomonas bacterium-immobilized product and method for immobilizing the pseudomonas bacterium, and method for preparing plant aboveground part disease control agent comprising the immobilized product |
JP5193626B2 (en) * | 2008-02-20 | 2013-05-08 | 日本曹達株式会社 | Preservation method of microbial pesticide preparation |
JP5302653B2 (en) * | 2008-12-18 | 2013-10-02 | 北興化学工業株式会社 | Microbial pesticide formulation with improved storage stability |
JP5685783B2 (en) * | 2012-01-19 | 2015-03-18 | 国立大学法人名古屋大学 | Novel Yarrowia microorganism, oil decomposing agent and oil decomposing / removing method using the same |
JP2013192490A (en) * | 2012-03-19 | 2013-09-30 | Research Institute Of Environment Agriculture & Fisheries Osaka Prefecture | Method for producing dried powder of microorganism |
-
2017
- 2017-03-31 JP JP2017070752A patent/JP6920089B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2018170988A (en) | 2018-11-08 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6674817B2 (en) | Wastewater treatment method and wastewater treatment kit | |
Abbasiliasi et al. | Fermentation factors influencing the production of bacteriocins by lactic acid bacteria: a review | |
US11352651B2 (en) | Method of isolating lipids from a lipids containing biomass | |
Maldonado et al. | A review on Geotrichum lipases: production, purification, immobilization and applications | |
JP6099054B2 (en) | Newly decomposed microorganisms of oils and fats and fatty acids | |
WO2018122057A1 (en) | Method of isolating lipids from a lipids containing biomass | |
JP2017176067A (en) | Production method of live bacterial preparation, live bacterial preparation, and wastewater treatment method using same | |
JP6920089B2 (en) | Production method of live bacterial product, live bacterial product and wastewater treatment method using it | |
JP2017136033A (en) | Oil content-decomposing microorganism | |
JP6343838B2 (en) | Wastewater treatment method and wastewater treatment kit | |
JP2017136032A (en) | Oil content-decomposing microorganism | |
TWI754146B (en) | Novel oil-decomposing microorganisms | |
US20230046934A1 (en) | Use of a defoamer for maintaining dispersed morphology in submerged fungal fermentation | |
Keum et al. | Effects of nutrients on quorum signals and secondary metabolite productions of Burkholderia sp. O33 | |
JP2018170996A (en) | Culture medium for yeast culture, and yeast culture method, and proliferation method using the same | |
JP7041010B2 (en) | Newly decomposing microorganisms of fats and oils | |
Gündüz | Lactic acid production by Lactobacillus casei NRRL B-441 immobilized in chitosan stabilized Ca-alginate beads | |
JP7109305B2 (en) | New oil-degrading microorganisms | |
JP7264699B2 (en) | Oil decomposition agent and oil decomposition method | |
JP7260369B2 (en) | New oil-degrading microorganisms | |
Tipre et al. | Production and characterization of lipase from Staphylococcus sp. SDMlip | |
JP6989987B1 (en) | Continuous input of microbial preparations for wastewater treatment containing oils and fats | |
Mohamed et al. | Fungal Lipids | |
JP2022180013A (en) | yeast medium | |
Muthazhagan et al. | Production and partial characterization of lipase by Bacillus sp. isolated from vellar estuary sediment |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A712 Effective date: 20180426 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20191105 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200901 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20200831 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20201030 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20210323 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20210513 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210720 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210726 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6920089 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |