CN113773994A - Efficient hydrolytic acidification microbial agent, preparation method and application - Google Patents
Efficient hydrolytic acidification microbial agent, preparation method and application Download PDFInfo
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- 230000004151 fermentation Effects 0.000 claims abstract description 4
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- 238000000034 method Methods 0.000 claims description 20
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- 239000002131 composite material Substances 0.000 claims description 4
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- 239000003223 protective agent Substances 0.000 claims description 4
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- 241000589220 Acetobacter Species 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
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- 241000235342 Saccharomycetes Species 0.000 claims description 3
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- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
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- 239000011780 sodium chloride Substances 0.000 claims description 2
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- 238000011161 development Methods 0.000 description 1
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- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
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- 150000004665 fatty acids Chemical class 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention discloses a high-efficiency hydrolytic acidification microbial agent, a preparation method and application, belonging to the technical field of environmental protection, and the high-efficiency hydrolytic acidification microbial agent mainly comprises the following components: the effective viable bacteria number of the microbial preparation prepared by the invention is 1 multiplied by 109～1×1011CFU/mL fermentation product. The prepared bacterial preparation is added into wastewater according to a certain amount, can be used in the front stage of a biochemical process for sewage treatment, and improves the biodegradability of sewage, so that a hydrolysis acidification technology can be used for replacing a physicochemical method for pretreatment, and the sewage is discharged after being treated.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and relates to a high-efficiency hydrolytic acidification microbial agent and a preparation method thereof.
Background
The hydrolysis acidification process in the sewage treatment process is one of important links of biological pretreatment, and plays an important role in each sewage treatment process. The hydrolysis acidification tank mainly has two basic functions: firstly, the biodegradability of sewage can be improved, and macromolecular organic matters are converted into micromolecules; secondly, the COD in the sewage can be removed, and partial organic matters are degraded to synthesize self cells. Compared with a separate aerobic process, the hydrolysis acidification process has the following advantages: suspended matters and organic matters in the sewage can be greatly removed, and the sludge amount of the subsequent aerobic treatment process is effectively reduced, so that the volume of equipment is reduced; creating stable water inlet conditions for aerobic treatment; low treatment cost, saving the operation cost of the whole process and the like.
However, in the installation and debugging stage of the current sewage treatment hydrolysis acidification process, the important work of the hydrolysis acidification tank is to culture sludge and perform artificial feeding nutrition culture according to needs, the feed water adopts sludge liquid pre-cultured by a front-stage sewage treatment plant, and the feed water is gradually fed according to the volume load of the sewage tank. Therefore, the following disadvantages exist: 1) the sludge culture period of the hydrolysis acidification tank is long, the debugging operation is complicated, and the operation is restricted by the professional degree of operators; 2) the treatment capability of the common hydrolysis acidification activated sludge is limited, and the requirements of the hydrolysis acidification process of the fine chemical wastewater with difficult treatment, high load and complex components can not be met.
Disclosure of Invention
The invention discloses a high-efficiency hydrolytic acidification microbial agent, a preparation method and an application method aiming at the problems in the prior art, can be used for the front stage of a biochemical process for sewage treatment, improves the biodegradability of sewage, and is used for solving the problem of difficulty in biotechnological treatment of fine chemical sewage.
The invention is realized by the following steps:
a preparation method of a high-efficiency hydrolytic acidification microbial agent is characterized by comprising the following steps:
respectively inoculating the obtained brevibacillus brevis, acetobacter, lysine bacillus, brevibacillus rosenbergii, serratia, saccharomycetes, bacillus licheniformis, pediococcus acidilactici and bacillus megaterium into an enrichment medium, and performing shake culture to a logarithmic phase;
step two, inoculating the seed liquid of each strain in the step one into a seed liquid culture medium according to the inoculation amount of 15% for amplification culture;
step three, fermenting the seed liquid obtained in the step two, and then mixing the fermented seed liquid with the fermented seed liquid according to the proportion of 1: 1: 1: 1: 1: 1: 1: 1: 1, mixing to form a compound microbial inoculum;
and step four, adding 20% of protective agent into the obtained composite microbial inoculum and preparing into dry powder microbial inoculum. (the formula of the protective agent comprises 35 percent of yeast powder, 35 percent of fish protein and 30 percent of trace elements by mass percentage)
Further, the preparation method of the enrichment medium in the first step is as follows: 3.0g/L of beef extract; peptone 10.0 g/L; NaCl 5.0 g/L; dissolving in deionized water, adjusting pH to 7.0, and sterilizing at 121 deg.C for 20 min.
Further, the preparation method of the liquid culture medium in the second step is as follows: 10g/L of yeast crude extract, 15g/L of peptone, 5g/L of glucose and NH4Cl 5g/L，K2HPO45g/L, dissolving in deionized water, adjusting pH to 7.0, and sterilizing at 121 deg.C for 20 min.
Further, the microbial agent prepared by the preparation method of the high-efficiency hydrolytic acidification microbial agent is characterized in that effective viable bacteria of the microbial agentThe number of the grooves is 1 multiplied by 109～1×1011CFU/mL fermentation product.
The invention also discloses application of the microbial agent prepared by the preparation method of the efficient hydrolytic acidification microbial agent in sewage treatment, wherein the application temperature is in the range of 15-30 ℃; the pH value of the application is 5.5-7.0; residence time: 2.5-4.5h, oxidation-reduction potential EhLess than or equal to 100 percent, and DO (dissolved oxygen) less than or equal to 0.5 mg/L.
Further, 1-3g of efficient hydrolytic acidification microbial agent is added into every 1L of sewage, a facultative mode is adopted for a hydrolytic acidification unit, granular activated carbon is used as a filler, and polyurethane is added in an auxiliary mode.
The beneficial effects of the invention and the prior art are as follows:
the hydrolysis acidification treatment method is an early stage of anaerobic treatment, anaerobic fermentation is controlled in hydrolysis and acidification stages, solid substances are degraded into soluble substances, macromolecular substances are degraded into micromolecular substances, and complex organic substances are degraded into fatty acids mainly comprising acetic acid, propionic acid and butyric acid through the action of hydrolysis and acid-producing bacteria, so that the pH value of the wastewater is reduced, and the wastewater presents certain acidity. By changing the structural form of the organic matters, some organic matters which are difficult to biodegrade are converted into biodegradable organic matters, so that the biodegradability of the wastewater is improved, and favorable conditions are created for subsequent biological treatment.
Based on the mechanism, the disclosed efficient hydrolytic acidification microbial agent can be used in the front stage of a biochemical process for sewage treatment to degrade macromolecular substances into micromolecular substances and convert certain organic substances which are difficult to be degraded into biodegradable organic substances, so that the biodegradability of sewage is improved, and favorable conditions are created for subsequent biological treatment. Therefore, the hydrolysis acidification technology can replace the physicochemical method for pretreatment, so that the sewage is discharged in a qualified way, the damage of the sewage to the ecological environment of the receiving water body is reduced, and the ecological environment is protected; driving the innovation of the environmental water treatment technology and promoting the rapid development of the environmental protection technology.
According to the invention, a plurality of strains with hydrolytic acidification functions are mixed according to a certain proportion, so that the hydrolytic acidification performance of the compound microorganism can be obviously improved; the strain product adopts a special fermentation process and a special nutrition formula, the viable count of the product exceeds 10^9, and the product can be directly added and used in engineering; after the strain product is put into a hydrolysis acidification process, the biodegradability of the wastewater can be greatly improved, and conditions are created for the stable operation of the subsequent sewage treatment process.
Drawings
FIG. 1 is a diagram showing the effect of the microbial agent for efficiently hydrolyzing and acidifying according to the present invention.
Detailed Description
In order to make the objects, technical solutions and effects of the present invention clearer and clearer, the present invention is further described in detail by the following examples. It should be noted that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Specific examples are as follows:
the preparation method of the high-efficiency hydrolytic acidification microbial agent comprises the following steps:
step 1: respectively inoculating the obtained brevibacillus faecalis, acetobacter, bacillus lysinate, brevibacterium rosenbergii, serratia, saccharomycete, bacillus licheniformis, pediococcus acidilactici and bacillus megaterium in an enrichment medium, and performing shake culture to a logarithmic phase;
step 2: inoculating the seed liquid of each strain in the step one into a seed liquid culture medium according to the inoculation amount of 15% for amplification culture;
and step 3: fermenting the seed liquid obtained in the step 2 and mixing to form a composite microbial inoculum;
and 4, adding 20% of protective agent into the obtained composite microbial inoculum, and preparing into dry powder microbial inoculum. The application of the high-efficiency hydrolytic acidification microbial agent comprises the following sequence and steps:
the temperature range of application is 15-30 ℃; the pH of the applied sewage is 5.5-7.0; residence Time (HRT): 2.5-4.5 h; oxidation reduction potential (E)h) Less than or equal to 100; DO (dissolved oxygen) is less than or equal to 0.5 mg/l.
1-3g of the efficient hydrolytic acidification microbial agent is added into every 1L of sewage, a facultative mode is adopted for a hydrolytic acidification unit, granular activated carbon is used as a main filler, and polyurethane is added in an auxiliary manner.
Example 1
The experimental verification is carried out by selecting certain pharmaceutical production sewage, the sewage has high pollutant concentration, complex components and high toxicity, contains various microorganism inhibiting components such as surfactant, residual antibiotic and the like, and belongs to antibiotic wastewater with certain treatment difficulty. The experiment adopts a hydrolytic acidification-aerobic process, and the water quality indexes are as follows:
the sewage experiment is diluted according to a certain proportion and then enters a hydrolysis acidification process, strains are added according to 0.1-3 per mill, process parameters are controlled according to requirements, a facultative mode is adopted by a hydrolysis acidification unit, granular activated carbon is used as a main filler, polyurethane is added in an auxiliary mode, an aeration mode is adopted by an aerobic unit, the polyurethane is used as a filler, and sampling is carried out at regular time to measure related indexes.
By using the high-efficiency hydrolytic acidification microbial agent, the sewage is BOD after hydrolytic acidification5The COD is increased from 0.15 to 0.43, the biodegradability of the sewage is greatly improved, the COD of the inlet water is 3445-3577mg/L, the COD of the hydrolysis acidification outlet water is 2645-2806mg/L, the COD of the aerobic outlet water is 215-231mg/L, and the COD removal rate of the whole process section reaches 92%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that modifications can be made by those skilled in the art without departing from the principle of the present invention, and these modifications should also be construed as the protection scope of the present invention.
Claims (8)
1. A preparation method of a high-efficiency hydrolytic acidification microbial agent is characterized by comprising the following steps:
respectively inoculating the obtained brevibacillus brevis, acetobacter, lysine bacillus, brevibacillus rosenbergii, serratia, saccharomycetes, bacillus licheniformis, pediococcus acidilactici and bacillus megaterium into an enrichment medium, and performing shake culture to a logarithmic phase;
step two, inoculating the seed liquid of each strain in the step one into a seed liquid culture medium according to the inoculation amount of 15% for amplification culture;
step three, fermenting the seed liquid obtained in the step two, and then mixing the fermented seed liquid with the fermented seed liquid according to the mass ratio of 1: 1: 1: 1: 1: 1: 1: 1: 1, mixing to form a compound microbial inoculum;
and step four, adding 20% of protective agent into the obtained composite microbial inoculum, and preparing into a dry powder microbial inoculum, wherein the proportion is mass percent.
2. The method for preparing a highly effective hydrolytic acidification microbial inoculum according to claim 1, wherein the enrichment medium in the first step is prepared by the following steps: 3.0g/L of beef extract; peptone 10.0 g/L; NaCl 5.0 g/L; dissolving in deionized water, adjusting pH to 7.0, and sterilizing at 121 deg.C for 20 min.
3. The method for preparing a high efficiency hydrolytic acidification microbial inoculum according to claim 1, wherein the liquid culture medium in the second step is prepared by the following method: 10g/L of yeast crude extract, 15g/L of peptone, 5g/L of glucose, 5g/L of NH4Cl 5, K2HPO45g/L, dissolving in deionized water, adjusting pH to 7.0, and sterilizing at 121 deg.C for 20 min.
4. The microbial agent prepared by the preparation method of the efficient hydrolytic acidification microbial agent according to any one of claims 1 to 3.
5. The microbial agent prepared by the preparation method of the microbial agent for efficient hydrolytic acidification according to claim 4, wherein the number of effective viable bacteria of the microbial agent is 1 x 109～1×1011CFU/mL fermentation product.
6. The application of the microbial agent prepared by the preparation method of the high-efficiency hydrolytic acidification microbial agent as claimed in claim 4 in sewage treatment.
7. The application of the microbial agent prepared by the preparation method of the efficient hydrolytic acidification microbial agent in sewage treatment according to claim 6, wherein the application temperature is in the range of 15-30 ℃; the pH value of the application is 5.5-7.0; residence time: 2.5-4.5h, oxidation-reduction potential EhLess than or equal to 100 percent, and DO (dissolved oxygen) less than or equal to 0.5 mg/L.
8. The application of the microbial agent prepared by the preparation method of the efficient hydrolytic acidification microbial agent in sewage treatment according to claim 6, wherein 1-3g of the efficient hydrolytic acidification microbial agent is added into 1L of sewage, a facultative mode is adopted for a hydrolytic acidification unit, granular activated carbon is used as a filler, and polyurethane is added in an auxiliary manner.
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| CN115010270A (en) * | 2022-06-15 | 2022-09-06 | 愉悦家纺有限公司 | Treatment method and treatment system for cotton printing and dyeing wastewater |
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| CN101538538A (en) * | 2008-03-18 | 2009-09-23 | 上海环伟生物科技有限公司 | Composite microorganism viable bacteria preparation and preparation method and application of same |
| CN105349471A (en) * | 2015-12-15 | 2016-02-24 | 金龙 | Preparation method of microbial flora preparation and application thereof |
| JP2018170988A (en) * | 2017-03-31 | 2018-11-08 | シーシーアイホールディングス株式会社 | Method for producing viable bacterial preparation, and viable bacterial preparation, and wastewater treatment method using the same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115010270A (en) * | 2022-06-15 | 2022-09-06 | 愉悦家纺有限公司 | Treatment method and treatment system for cotton printing and dyeing wastewater |
| CN115010270B (en) * | 2022-06-15 | 2024-01-26 | 愉悦家纺有限公司 | Treatment method and treatment system for cotton printing and dyeing wastewater |
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Application publication date: 20211210 |