JP6660519B1 - Elastase inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an elastase inhibitor which can be used for foods and drinks, medicines and the like for improving the original function of skin tissue to prevent and / or improve skin troubles and promote beautiful skin. An elastase inhibitor and an oral composition comprising an enzymatic decomposition product, an extract, an extraction residue or sprout of a mature seed of okra (Abelmoschus esculentus). [Selection diagram] None

Description

The present invention relates to an elastase inhibitor characterized by comprising a processed product of okra ( Abelmoschus esculentus ) seeds and use thereof.

Animal and fish biological tissues generally hold epithelial tissues such as the epidermis of the skin and the surface of organs, muscular tissues that mainly constitute muscles, nervous tissues that span the whole body, and body structures. It is roughly divided into supporting connective tissue. Connective tissue is also called support tissue, and in a broad sense, bone, cartilage, blood, fat, and the like may be classified as connective tissue.

Of these, epithelial tissue and supporting tissue have been studied and developed for a long time in skin, bone, fat, etc. In recent years, for example, as a part of skin aging symptoms, skin tension and decrease in elasticity (wrinkles, The mechanism of generation of sagging and the like is being elucidated, and it is said that elastin (elastic fiber) is greatly involved in wrinkles and sagging of the skin.

Elastin is a type of fibrous high-molecular protein that exists in connective tissues.It binds collagen, which is a high-molecular protein, to each other in a lattice pattern, and contains mucopolysaccharides such as hyaluronic acid and chondroitin sulfate in the gaps. Various extracellular components form an extracellular matrix structure. This extracellular matrix structure supports cells and skin tissue, retains water in the intercellular space, maintains skin lubricity and flexibility, and protects skin tissue from external factors such as ultraviolet light, dry environment, mechanical irritation and damage, and microbial infection. It has a role to protect and others. It is known that these proteins and extracellular components are produced in vivo by fibroblasts (Non-Patent Document 1).

It is generally recognized that the elastin content in living tissue is about 78-80% in the ligament ligament, about 50% in the artery, about 20% in the lung, and about 2-5% in the dermis. One of the features of elastin is that, unlike collagen and hyaluronic acid, the amount increases with growth at almost 0% at the time of birth of a human, and reaches a peak in the mid-20s. It is said that the number will begin to decrease and will fall below 50% after the age of 40s. This suggests that elastin in living tissues may be degraded or denatured by metabolic changes with age, ultraviolet light, or other factors, and this may be due to the increased or excessive activity of elastin, an enzyme that degrades elastin. Guidance is assumed.

From such a viewpoint, it is disclosed that an aqueous component obtained from okra seeds is used as an active ingredient for promoting the proliferation of fibroblasts (Patent Document 1). In addition, various studies have been made to prevent the content of elastin and collagen in the dermis from being reduced, and various reports and proposals have been made. As an example, an active ingredient of a cosmetic that improves skin aging (wrinkles and elasticity) A retinol glycoside is disclosed (Patent Document 2). However, retinol derivatives are also known to have side effects due to excessive administration, which is not sufficient in terms of safety.

In addition, many attempts have been made to search for substances that inhibit the activity of elastase. So far, as a plant-derived component or an extract, an extract of sugarcane (Patent Document 3) and a phenylethanoid glycoside (echinakoside and acteoside) have been used. Extracts of Antelidae plants (Patent Literature 4), Resveratrols extracted from grape sprouts and vines (Patent Literature 5), Pomegranate flower powder and / or extract (Patent Literature 6), and extraction of Mannaketake Products (Patent Document 7), soybean protein hydrolysates (Patent Document 8), and the like.

It is described that these components and extracts are used in cosmetics and external preparations for skin, applied to the skin and used. However, when used in cosmetics or external preparations for the skin, there are questions and difficulties in the percutaneous absorption of the components and easy cleaning properties, and the desired effects as measures against skin aging symptoms are not sustained. Did not substantially improve the physiological function of In addition, when peptides are orally ingested, there is a risk of deterioration or degradation in the gastrointestinal tract, and few of them can exhibit effectiveness in practical use. Therefore, there is a need for an effective material that can improve the above-mentioned skin aging symptoms.

Okra ( Abelmoschus esculentus ) is a plant belonging to the genus Troloa mallow of the family Malvaceae , and has a long history of growing in tropical to temperate regions of the world, cultivated as vegetables, and provided for food. Usually, fruits (pods) containing immature seeds of white or yellowish white are fed as fresh vegetables.

As an attempt to industrially use extracts and components obtained by processing okra, the above-mentioned Patent Document 1, a hyaluronic acid synthesis promoter containing an okra extract, and a cosmetic or food or beverage containing the agent (Patent Document 9) ), An antiaging skin external preparation containing an okra seed-derived oligopeptide and a specific plant extract (Patent Document 10). However, there is no mention of the relationship between okra seeds or processed products and elastin or elastase.

Michihiro Hattori, "Science of Skin Care", pp. 6-14 and pp. 15-83, Shokabo Co., Ltd., published February 25, 1997

JP, 2018-115143, A JP-A-10-158290 (Claim 1 etc.) JP 2012-201615 A, JP 2009-263279 A JP 2008-239576 A JP 2005-53873 A JP 2005-23021 A JP 2004-182687 A JP 2004-51533 A JP 2008-74757 A

In view of the current situation, the present invention provides an elastase for restoring the reduction of elastin in living tissues caused by external factors such as ultraviolet rays and active oxygen, a decrease in metabolic function of a living body, and the like, and improving skin aging symptoms and the like. An object of the present invention is to provide an activity inhibitor and an industrially usable composition.

In order to solve the above-mentioned problems, the present inventors have conducted intensive studies on a material that inhibits the activity of fibroblast-derived elastase and a method for processing the same, and found that a processed product of okra seeds is effective. As a result, the present invention has been completed.

That is, the main features of the present invention generally reside in the following points.
(1) An elastase inhibitor comprising a processed product of okra ( Abelmoschus esculentus ) seed.
Here, the seeds are preferably ripe seeds, the processed product is preferably an okra seed extract, the extraction residue, an enzymatically decomposed product or a sprout, and the extraction is performed using hot water extraction. The enzymatic degradation treatment is preferably hydrolysis using an alkaline protease and / or a neutral protease, and the elastase is preferably derived from skin fibroblasts.

(2) A method for inhibiting elastase activity, comprising orally ingesting the elastase inhibitor.

(3) An oral composition comprising the above elastase inhibitor.
Here, the oral composition is desirably in the form of a food or drink, and desirably for increasing elastin based on the elastase activity inhibitory action of skin tissue, and furthermore, aging of skin tissue. It is desirable that it is for improvement.

The hot water extract, a residue thereof, a protein hydrolyzate and sprouts according to the present invention, which is a processed product obtained from ripe seeds of okra, has a strong inhibitory effect even on a small amount against elastase derived from skin fibroblasts. Play. For this reason, the esterase inhibitor of the present invention containing the processed product promotes skin turnover, improves skin troubles and aging symptoms (wrinkles, spots, dullness, freckles, sagging, etc.), and contributes to promoting beautiful skin. It becomes possible. In addition, it can be expected to enhance elastin in the skin tissue and to help maintain skin health, for example, to promote regeneration of damaged skin sites.
Such effects are remarkably exhibited by ingesting or administering the elastase inhibitor of the present invention orally.
Therefore, the elastase inhibitor of the present invention can be effectively used for skin improvement, particularly in the fields of foods and drinks, pharmaceuticals, animal feeds, and the like, in the form of the agent or in a form mixed with various conventional products in the field. It is possible to do.

  Hereinafter, the present invention will be described in detail. First, the elastase inhibitor of the present invention has an action of inhibiting elastase, which is an enzyme that degrades elastin present in living tissues, particularly dermal tissues of the skin, and comprises a processed product of okra seeds. It is characterized by the following.

  In general, there are varieties of okra whose pod shape is pentagonal, octagonal, round, and the like, and varieties whose color is green, purple, white and the like. The okra according to the present invention is not limited to these types, and any type can be used.

  In the present invention, it is desirable to use a ripe seed of the okra as a raw material. Ripe seeds are seeds that are harvested or spontaneously scattered from fruits (pods) in which plants have grown, and are dark green to dark brown with a diameter of about 5 mm and germinate when sown. Have the ability. On the other hand, immature seeds are contained in growing pods, are white to yellowish white and have a diameter of about 2 to 4 mm, and are usually edible with the pods. Mature seeds of okra are commercially available from a seed company for growing okra, and it is convenient to use this.

The okra seeds are desirably collected as a processed product described below, that is, an extract of the okra seeds, an extraction residue, an enzymatic hydrolyzate, or sprout.

The extract of okra seeds can be produced, for example, by the following processing.
The moulted or non-moulted seeds are coarsely or finely pulverized, and water is added to form a dispersion, which is then subjected to an extraction treatment at room temperature or under heating, standing, or appropriately stirring. Then, the extract was centrifuged and the insoluble matter was removed by treatment with conventional methods Filtration like, more preferably by concentration and drying or freeze-drying at room temperature or less cold, the present invention Such an extract can be produced.

  In the above-mentioned extraction step, when producing a hot water extract, the aqueous dispersion is set at about 30 to about 90 ° C, more preferably at about 40 to about 60 ° C, and for about 10 minutes to several days. Preferably, the extraction treatment is performed for about 30 minutes to several hours, and in the case of producing a low-temperature extract, the temperature is set to about 10 to about 30 ° C, more preferably about 15 to about 25 ° C, and about 30 minutes to several days, more preferably. May be immersed for several hours to one day to perform the extraction process.

  The extraction residue of okra seeds can be produced by concentrating and drying or freeze-drying the insoluble matter removed in the above-mentioned extraction step by a conventional method. In this case, the degree of drying is preferably set to a water content of 5% by mass or less in order to prevent contamination due to generation of mold and the like. The residue of the present invention is desirably a residue separated at the time of producing the aforementioned low-temperature extract (low-temperature extraction residue) in relation to the desired effects of the present invention.

The enzymatically decomposed product of okra seeds can be produced by processing as follows. That is, okra seeds are pulverized into an aqueous dispersion, and about 0.1 to about 5% by mass, more preferably about 0.5 to about 2% by mass of protein hydrolase per seed is added thereto. At room temperature or under heating (about 30 to about 90 ° C., more preferably about 40 to about 60 ° C.), the mixture is left standing or appropriately stirred for about 10 minutes to several days, more preferably 30 minutes to several hours. Decomposition processing is performed. After the treatment, by a conventional method enzyme heat deactivation, then separated by centrifugation or filtration over treated insolubles, preferably room temperature or less concentrated in a low temperature, it is prepared by drying or freeze-drying process be able to. In the present invention, instead of the aqueous dispersion, using an okra seed extract or extraction residue obtained by treating as described above dissolved or dispersed in water, similarly enzymatically treated Is also good.

  The protease may be of any type or type. In the present invention, an alkaline protease and / or a neutral protease are preferred, and an alkaline protease is more preferred. Here, the alkaline protease can be used as long as it has an action at a substrate pH of about 6.5 to 12, and the neutral protease is an action at a substrate pH of about 5 to 8. Anything is fine. Each protease may be used alone or in combination of two or more, and it is convenient to use a commercially available product as described below.

Specific examples of the alkaline protease include the following. However, the present invention is not limited by these.
"Protin SD-AY10", "Protease P" Amano "3SD", "Proleather (registered trademark) FG-F" (all manufactured by Amano Enzyme Co., Ltd.), "Alcalase (registered trademark) 2.4 LFG" (Novozymes) "Orientase (registered trademark) 22BF" (manufactured by HBI Co., Ltd.), "Aloase (registered trademark) XA-10" (manufactured by Yakult Pharmaceutical Co., Ltd.), "Sumiteam (registered trademark) MP" (manufactured by Yakult Pharmaceutical Co., Ltd.) Nippon Chemical Industry Co., Ltd.), "Bioprase OP, SP-20FG, AL-15FG, 30G, APL-30 and 30L" (manufactured by Nagase ChemteX Corporation), "OPTIMASE (registered trademark) PR89L", " MALTIFECT (registered trademark) PR6L "(both manufactured by Danisco US)," Proteinase K "," Chymotrypsin "(both manufactured by Rosi Company, Ltd.), and the like.

The following can be exemplified as the neutral protease, but the present invention is not limited thereto.
"Flavourzyme (registered trademark)", "PROTAMEX (registered trademark) MG", "Neutrase" (all manufactured by Novozymes), "Punchase (registered trademark) P and MP", "Aloase (registered trademark) AP-10, NP -10 and NS "(all manufactured by Yakult Yakuhin Kogyo Co., Ltd.)," Nucleicin (registered trademark) "," Orientase (registered trademark) 10NL and 90N "(all manufactured by HBI Co., Ltd.)," Protin P " (Manufactured by Daiwa Kasei Co., Ltd.), "Protease A" Amano "G", "Papain W40", "Bromelain F" (manufactured by Amano Enzyme Co., Ltd.), "Sumiteam (registered trademark) LP and LPL" (new Nippon Kagaku Kogyo Co., Ltd.), "Purified Papain for food", "Denateam AP" (above, manufactured by Nagase ChemteX Corporation), "Trip Down "(manufactured by Roche), and the like.

The sprout of okra seeds is germinated by a known method, grown in a karyotype, harvested after cultivation until green leaves are formed and the stem length is about 5 to 15 cm, washed with water, dried or freeze-dried. It can be manufactured by doing.

The processed product obtained by the treatment as described above includes proteins, polysaccharide protein complexes, polysaccharides, etc. contained in okra seeds and / or peptides, amino acids, oligosaccharides, monosaccharides or oligosaccharides obtained by hydrolyzing them. It is speculated that the composition is an extremely complex composition containing various components such as a conjugate of a saccharide and a peptide or an amino acid.

  In the present invention, the processed product of the okra seed as it is, or in combination with a known excipient such as dextrin, cellulose, gelatin, purified water, liquid, powder, granule, capsule-like of the present invention An elastase inhibitor can be prepared. The content of processed okra seeds in this agent is about 20% by mass or more. If the content is less than 20% by mass, the desired effect of the present invention is hardly exhibited. 100 to about 20/0 to about 80 is desirable, and 100 to about 50/0 to about 50 is even more desirable.

  The elastase inhibitor of the present invention, by ingesting or administering it orally, suppresses elastase action in the skin tissue to prevent elastin reduction, thereby improving the above-mentioned troubles in the skin and recovering aging symptoms. It can be used as a beauty means for promoting beautiful skin.

  In a preferred embodiment, the elastase inhibitor of the present invention is formed into a solid (powder or granule), gel (jelly), paste or liquid (beverage or drink) product such as food and drink, pharmaceutical product, animal feed and the like. It is possible. In addition, known additives (surfactants, thickeners, antioxidants, coloring agents, fragrances, etc.) used for producing these products and known materials (fibroblasts) which do not contradict the spirit of the present invention. Cell growth promoting action, action of promoting production of extracellular components such as collagen and hyaluronic acid, skin aging preventing action, skin and skin promoting action, etc.) are appropriately used in combination with known animal and plant materials to form the above-mentioned various products by a conventional method. It is possible.

Next, the present invention will be described in detail with reference to examples, but the present invention is not limited thereto. In the following, parts and percentages are by mass unless otherwise specified.

Production Example 1
Ripe seeds of Okra from Ibusuki, Kagoshima Prefecture are coarsely pulverized with a mill, 300 mL of distilled water is added to 100 g of this, and the mixture is heated to 58 ° C. with stirring to prepare a dispersion (pH 7.5). One part of Orientase 22BF (manufactured by HBI Co., Ltd., alkaline protease) was added, and stirring was continued gently for 5 hours. Thereafter, the enzyme was deactivated (80 ° C. for 30 minutes), insolubles were removed by centrifugation, and lyophilized to obtain an enzyme digest (sample 1).

Comparative sample 1
As Comparative Sample 1, a commercially available product from litchi seed (trade name: “Litch Seed Extract-WSP”, manufactured by Oriza Yuka Co., Ltd.), which is a material known to have an elastase inhibitory action, was used.
Comparative sample 2
As Comparative Sample 2, a commercially available resveratrol-containing composition (trade name: “Resveratrol-ε”), which is a material known to have an elastase inhibitory effect, was used.

Comparative sample 3
As Comparative Sample 3, a commercially available button-bofu leaf powder (trade name: “Button-bohu leaf powder”, manufactured by BCH Co., Ltd.), which is a known plant-derived material, was used.

Comparative sample 4
As Comparative Sample 4, a commercially available sesame leaf powder (trade name: “sesame leaf powder”, a known material derived from a plant, manufactured by BCH Co., Ltd.) was used.

Comparative sample 5
As Comparative Sample 5, a commercially available product of okra seed powder, which is a known material derived from okra (seller: Matsumoto Trading Co., Ltd., trade name: “Myoxinol LS 9736”) was used.

Production Example 2
The mature seeds used in Production Example 1 were roughly pulverized, 800 mL of water was added to 100 g, and the mixture was heated at 80 to 90 ° C. for 60 minutes, cooled to room temperature, and subjected to microfiltration (filter paper) to collect a filtrate. 600 mL of water was again added to the filtration residue, and the same treatment was carried out to collect a filtrate. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to produce an extract (sample 2).

Production Example 3
The mature seeds used in Production Example 1 were coarsely pulverized, 800 mL of water was added to 100 g, and the resultant was immersed at 15 to 25 ° C. overnight, then subjected to microfiltration (filter paper) to collect a filtrate, concentrated under reduced pressure, and lyophilized. Then, the mixture was pulverized to produce an extract (sample 3).

Production Example 4
The residue after filtration described in Production Example 3 was freeze-dried and pulverized to produce an extraction residue (Sample 4).

Production Example 5
The same ripe seeds as in Production Example 1 were immersed in water at 0 ° C. to room temperature for 8 to 20 hours to remove water, sowed in a cultivation container, and stored in a dark room at room temperature to 30 ° C. and 75 to 90% humidity, Germination was carried out by spraying water appropriately. After germination, the sprouts were transferred to a house and exposed to sunlight at room temperature of 25 to 30 ° C. and sprinkled with water as appropriate to obtain a sprout of the type after 5 to 7 days. This was washed with water, freeze-dried and pulverized to produce a pulverized sprout (sample 5).

Comparative Production Example 1
The same treatment as in Production Example 1 was carried out except that the mature seeds were replaced with immature seeds, thereby obtaining an enzymatically degraded product (Comparative Sample 6).

Comparative Production Example 2
Same as Production Example 1, except that the ripe seed was replaced with a commercial product (product name: “Okra Powder”) derived from an edible portion of okra (a pod containing immature seed; the same applies hereinafter) derived from ale. To obtain an enzyme degradation product (Comparative Sample 7).

Comparative Production Example 3
An extract (Comparative Sample 8) was prepared in the same manner as in Production Example 2, except that the ripe seed was replaced with a commercial product derived from the edible portion of Okra (manufactured by Yale, trade name: “Okra Powder”). Obtained.

Test Example The effect of each sample on elastase activity was examined by the method described below.

Test Example 1: Elastase inhibitory action (1)
The above-mentioned sample 1 and comparative samples 1 to 3 were used as test samples for measuring elastase activity. Each test sample was dissolved in 100 mM Tris / hydrochloric acid buffer (pH 8.0) to prepare a test solution having a final concentration of 40.0 to 1000.0 μg / ml. At this time, if it was difficult to dissolve, DMSO was added within 10%.
25 μl of this test solution and a human skin fibroblast-derived elastase solution (here, the elastase solution was precultured in a 96-microplate so that human skin fibroblasts (manufactured by Kurabo Industries, Ltd.) became confluent. After culturing for 24 hours in a DMEM medium containing% FBS, the cells were washed twice with PBS (-), added with 0.1% triton X-100, and dissolved by sonication, and the lysate was centrifuged at 12,000 rpm for 5 minutes. Then, the supernatant was recovered, and the lyophilized powder was used as a crude enzyme powder, and dissolved in a buffer in each test.) 25 μl was mixed and pre-warmed at 37 ° C. for 5 minutes.
As a control, a mixed solution obtained by adding only 100 mM Tris / hydrochloric acid buffer and DMSO to the elastase solution was similarly treated.
Next, 50 μl of a solution containing N-succinyl-trialanyl-P-nitroanilide as a substrate (the substrate was dissolved in a minimum amount of DMSO, and 100 mM Tris / hydrochloric acid buffer (pH 8.0) was added to make 5 mM) was added. The reaction was started. After 2 hours, nitroaniline released by the action of elastase was measured as 405 nm wavelength light using a spectrophotometer (Microplate Reader SH9000 Lab, manufactured by Corona Electric Co., Ltd.), and a control sample to which no test sample was added was used. The elastase inhibition rate was calculated from the absorbance of the sample relative to the absorbance. The elastase inhibition rate was determined by the following formula.
Elastase inhibition rate (%) = {1− (control−sample) / (control−blank)} × 100

Table 1 shows the results. In the same table, the degree of elastase inhibition rate derived from fibroblasts was expressed as a relative value when the value of a control test performed at the same time was set to 0.
From the data shown in the table, when the ripe seed was treated with the enzyme (sample 1), an extremely high elastase inhibitory action was exhibited at any concentration of the test sample. In contrast, the lychee seed extract (Comparative Sample 1) and the resveratrol-containing composition (Comparative Sample 2) whose elastase inhibitory activity is already known have an increased inhibitory effect at a high concentration but an inhibitory effect at a low concentration. It was confirmed that the action was small, and that the plant-derived button botanical leaf powder (Comparative Sample 3) and the sesame leaf powder (Comparative Sample 4) had a weak inhibitory effect at any concentration.

Test Example 2: Elastase inhibitory action (part 2)
In this test example, the effects of processed products other than enzymatically degraded okra seeds and processed products other than ripe seeds on the elastase inhibitory action were examined. Here, the measurement of elastase inhibitory activity and the evaluation of the degree of the inhibitory effect were carried out in the same manner as in Test Example 1, but the final concentration of the test solution was set to 0.4 to 10.0 μg / ml. The test samples used were Sample 1, Samples 2 to 5, and Comparative Samples 5 to 8 .

Table 2 shows the results. In the same table, the degree of elastase inhibitory action (inhibition rate) was expressed as a relative value when the value of a control test performed at the same time was set to 0.
From the data in the table, the extract (samples 2 and 3), the extraction residue (sample 4), and the sprout (sample 5), which are the processed products of ripe seeds of okra, are as strong as the enzyme digest (sample 1). On the other hand, in the case of powder derived from okra seeds (Comparative sample 5), the elastase-inhibiting effect is inferior, and when the immature seed is enzymatically degraded (Comparative sample 6), the edible portion (pod containing immature seed) ) Was treated with enzyme (Comparative Sample 7) and extracted with hot water (Comparative Sample 8), it was confirmed that almost no elastase inhibitory action was observed.

Test Example 3: Human Monitor Test 40 adult female volunteers (30 to 55 years old, average age:
(44.5 years old) were divided into 20 groups, and one group received sample 1 (100 mg) and the other group received placebo (dextrin 100 mg) orally twice a day. Lasted a week. Changes in the skin before and after ingestion were evaluated using a questionnaire survey on the presence or absence of improvement using items such as skin dryness, spots, wrinkles, astringent, freezing, sagging, spine lines, makeup paste, and pore openings. .

As a result, in the sample 1 group, the skin improved as compared to before the ingestion: 17 people, did not change: 2 people, one person who had deteriorated, and in the placebo group, the skin improved: 2 people, did not change : 14 people, 4 people who got worse. The 17 individuals with changed skin had two or more choices of improvement, especially in terms of wrinkles, swelling, sagging and laugh lines. Also selected were improvements in skin dryness and pore opening that were not found in the placebo group.
The results suggest that the mature seeds according to the present invention are useful for improving skin by inhibiting elastase.

Trial Production Example 1: Hard Capsule Formulation Sample 1 was supplied to a capsule filling machine, and a gelatin-coated hard capsule formulation having a content of 100 mg per particle was trial-produced by a conventional method. When a hard capsule preparation (comprising the ripe okra seeds of Production Example 1) was orally ingested in a monitor test, a finding useful for improving the skin was obtained. Therefore, the tablet can be used as a dietary supplement that can be taken orally for improving the skin, a pharmaceutical product, or an animal feed.

Trial Production Example 2: Tablet The following raw materials were tableted by a conventional method to produce a trial tablet. Here, any one of the aforementioned samples 1 to 5 was used as the ripe okra seed. All of these tablets are stable and easy to take, and can be used as dietary supplements and pharmaceuticals.
(Ingredients) (Mass (mg) per tablet)
1. Okra ripe seed 100
2. Dextrin 100
3. Potato starch 49
4. Microcrystalline cellulose 20
5. Synthetic aluminum silicate 30
6. Calcium stearate 1

Trial Production Example 3: Dietary Supplements The sample 1 was 100 mg, leucine 400 mg, isoleucine 250 mg, valine 200 mg, and arginine 300 mg. This dietary supplement can be used not only for humans but also for pet foods.

Prototype example 4: Food (noodles)
10 g of the above-mentioned sample 1, 350 g of buckwheat flour and 150 g of strong flour were sieved while mixing these, and 150 ml of water was mixed to produce a buckwheat trial. This did not have a sense of incongruity in flavor and texture compared to the commercial product.

Prototyping Example 5: Soft drinks and drinks 500 mg or 100 mg of the sample 1 or 2 was added to 100 mL of commercially available soft drinks and drinks, and mixed well to prepare a drink. This beverage was comparable in flavor and storage stability to the original soft drink and drink. It can be used as a soft drink or drink.

Processed products obtained from mature seeds of okra have the action of inhibiting elastase activity derived from fibroblasts.By ingesting or administering them orally, the original physiological function of the skin is restored, and skin troubles are restored. It can be effectively used as foods and drinks, medicines, animal feeds, etc. that are useful for improving skin quality, promoting beautiful skin, and promptly recovering skin damage.

Claims (4)

An oral composition for inhibiting elastase derived from skin fibroblasts, comprising a processed product of ripe seeds of okra (Abelmoschus esculentus). The composition according to claim 1, wherein the processed product is an extract of okra seeds, an extraction residue, an enzymatic degradation product, or a sprout. 3. The composition according to claim 2, wherein the extract is a hot water extract. The composition according to claim 2, wherein the enzymatic degradation product is a hydrolysis product of an alkaline protease and / or a neutral protease.