JP6611400B2 - 間葉系幹細胞又は培養上清を含むシノビオリン発現阻害剤 - Google Patents
間葉系幹細胞又は培養上清を含むシノビオリン発現阻害剤 Download PDFInfo
- Publication number
- JP6611400B2 JP6611400B2 JP2018547809A JP2018547809A JP6611400B2 JP 6611400 B2 JP6611400 B2 JP 6611400B2 JP 2018547809 A JP2018547809 A JP 2018547809A JP 2018547809 A JP2018547809 A JP 2018547809A JP 6611400 B2 JP6611400 B2 JP 6611400B2
- Authority
- JP
- Japan
- Prior art keywords
- mesenchymal stem
- synoviolin
- stem cells
- culture supernatant
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/104—Aminoacyltransferases (2.3.2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/02—Aminoacyltransferases (2.3.2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Hematology (AREA)
- Epidemiology (AREA)
- Physics & Mathematics (AREA)
- Rheumatology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Description
リウマチ以外にも,肺,肝臓などにおいてシノビオリンがコラーゲンの過剰な分泌を促し,結果として繊維化を引き起こすことも明らかとなっている(非特許文献3,4)。また,シノビオリンが抗がん遺伝子p53の分解に関わることから,シノビオリンが発がんを引き起こす可能性も示唆されている(非特許文献5)。
まず,脂肪由来の間葉系幹細胞を,バイオミメティクスシンパシ−ズ社製の間葉系幹細胞用無血清培地(sf−DOT培地)で3日間培養し,培養上清を得た。
本実施例では,脂肪由来,臍帯由来及び羊膜由来の間葉系幹細胞培養上清が,リウマチ患者に由来する滑膜細胞のシノビオリン発現を抑制する活性を測定した。まず,脂肪由来の間葉系幹細胞を,sf−DOT培地で3日間培養し,培養上清を得た。リウマチ患者より採取した滑膜細胞を,6ウェル培養プレ−トに1x105/wellで播種した。
DBA/1マウス(体重は25グラム程度)を用いたコラーゲン誘発関節炎モデルに対するヒト脂肪由来間葉系幹細胞の影響を調べた。DBA/1マウスの尾根部皮内に,アジュバンドと混合したウシII型コラーゲンを投与し,感作を行った。初回コラーゲン感作2週間後に,再度のコラーゲン感作を行い,関節炎を作製した。間葉系幹細胞は,初回のコラーゲン感作を行う2週間前から,1週間に2回の頻度で継続的に9週間にわたって,1x106細胞を背部の皮内に投与した。観察期間中は,後肢の厚みをノギスで測定した。
本実施例では,脂肪由来,臍帯由来及び羊膜由来の間葉系幹細胞培養上清が,一般的に研究に使用される細胞株のシノビオリン発現を抑制する活性を測定した。まず,脂肪由来の間葉系幹細胞を,sf−DOT培地で3日間培養し,培養上清を得た。A549,HEK293およびHeLa細胞を,6ウェル培養プレ−トに1x105/wellで播種した。
Claims (2)
- 間葉系幹細胞又は間葉系幹細胞の培養上清を有効成分として含み,シノビオリン遺伝子の発現又はシノビオリンタンパク質の発現を阻害するシノビオリン発現阻害剤。
- 間葉系幹細胞を培養し,間葉系幹細胞又は間葉系幹細胞の培養上清を得る工程と,
前記工程で得られた間葉系幹細胞又は間葉系幹細胞の培養上清を用いて,シノビオリン遺伝子の発現又はシノビオリンタンパク質の発現を阻害するシノビオリン発現阻害剤を調整する工程と,
を含む,シノビオリン発現阻害剤の製造方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016213580 | 2016-10-31 | ||
JP2016213580 | 2016-10-31 | ||
PCT/JP2017/039013 WO2018079753A1 (ja) | 2016-10-31 | 2017-10-27 | 間葉系幹細胞又は培養上清を含むシノビオリン発現阻害剤 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2018079753A1 JPWO2018079753A1 (ja) | 2019-10-10 |
JP6611400B2 true JP6611400B2 (ja) | 2019-11-27 |
Family
ID=62025086
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2018547809A Active JP6611400B2 (ja) | 2016-10-31 | 2017-10-27 | 間葉系幹細胞又は培養上清を含むシノビオリン発現阻害剤 |
Country Status (5)
Country | Link |
---|---|
US (1) | US20190255117A1 (ja) |
EP (1) | EP3533801A4 (ja) |
JP (1) | JP6611400B2 (ja) |
CN (1) | CN110022888A (ja) |
WO (1) | WO2018079753A1 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022004823A1 (ja) * | 2020-06-30 | 2022-01-06 | 国立大学法人高知大学 | 臍帯血単核球細胞の培養上清を含む脳神経障害治療剤 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5139294B2 (ja) | 1973-03-23 | 1976-10-27 | ||
JPS5262712A (en) | 1975-11-20 | 1977-05-24 | Agency Of Ind Science & Technol | Axial flow blower |
AU2002216399B9 (en) | 2000-12-22 | 2005-12-01 | St. Marianna University School Of Medicine | Synovial cell protein |
WO2005089800A1 (ja) * | 2004-03-17 | 2005-09-29 | Locomogene, Inc. | hsHRD3を含む医薬組成物 |
JPWO2006137514A1 (ja) * | 2005-06-23 | 2009-01-22 | 学校法人 聖マリアンナ医科大学 | シノビオリンの発現もしくは機能阻害物質を有効成分とする癌治療剤、および癌治療剤のスクリーニング方法 |
JP2009155204A (ja) | 2005-12-20 | 2009-07-16 | Locomogene Inc | アレルギー性疾患用医薬組成物 |
KR101107835B1 (ko) | 2006-01-13 | 2012-02-09 | 가부시키가이샤 투셀 | 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도 |
JP2009055866A (ja) * | 2007-08-31 | 2009-03-19 | Univ Kinki | 滑膜組織由来の幹細胞および細胞株ならびにその濃縮培養方法 |
CN101412985B (zh) * | 2007-10-15 | 2012-06-13 | 华东理工大学 | 用于骨髓间充质干细胞体外培养和扩增的无血清培养基 |
CN102791276B (zh) * | 2010-03-10 | 2015-03-04 | 智再如股份有限公司 | 含有间充质干细胞的细胞制品及其制造方法 |
JP5993113B2 (ja) | 2011-01-31 | 2016-09-14 | 株式会社 バイオミメティクスシンパシーズ | アルツハイマー病治療に向けたヒト脂肪組織由来間葉系幹細胞 |
KR102260122B1 (ko) * | 2014-03-11 | 2021-06-03 | 훗카이도 코리츠 다이가쿠 호진 삿포르 이카 다이가쿠 | 간엽줄기세포 활성화제, 활성화된 간엽줄기세포 및 그 제조 방법 |
CN105754937A (zh) * | 2016-03-31 | 2016-07-13 | 北京大学第三医院 | 一种促进软骨分化的细胞共培养体系及其制备方法 |
-
2017
- 2017-10-27 US US16/346,112 patent/US20190255117A1/en not_active Abandoned
- 2017-10-27 EP EP17865765.6A patent/EP3533801A4/en not_active Withdrawn
- 2017-10-27 JP JP2018547809A patent/JP6611400B2/ja active Active
- 2017-10-27 WO PCT/JP2017/039013 patent/WO2018079753A1/ja unknown
- 2017-10-27 CN CN201780074254.XA patent/CN110022888A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
CN110022888A (zh) | 2019-07-16 |
WO2018079753A1 (ja) | 2018-05-03 |
JPWO2018079753A1 (ja) | 2019-10-10 |
US20190255117A1 (en) | 2019-08-22 |
EP3533801A4 (en) | 2020-06-17 |
EP3533801A1 (en) | 2019-09-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK2194121T3 (en) | CELL GROWTH PROCESS AND PHARMACEUTICAL COMPOSITION FOR tissue repair and regeneration | |
US20200377859A1 (en) | Umbilical cord mesenchymal stem cells (mscs) and culture method and application thereof | |
KR101788885B1 (ko) | 세포 요법에 사용하기 위한 방법 및 조성물 | |
US20190276803A1 (en) | Method of culturing immune cells, kit for thereof, immune cell cultured medium obtained by same method, cosmetic composition and pharmaceutical composition comprising thereof | |
US20150274792A1 (en) | Novel Method for Treating Spinal Cord Injury Using HMGB1 Fragment | |
Fan et al. | Cell therapy of a knee osteoarthritis rat model using precartilaginous stem cells. | |
JP6611400B2 (ja) | 間葉系幹細胞又は培養上清を含むシノビオリン発現阻害剤 | |
JP2017093431A (ja) | 脂肪由来間葉系幹細胞、脂肪由来間葉系幹細胞の培養方法、及び治療薬 | |
CN111904980B (zh) | 间充质干细胞与在治疗急性肺损伤、急性呼吸窘迫症或肺纤维化中的用途 | |
JP2017536849A (ja) | 幹細胞材料およびその製造方法 | |
KR20190074720A (ko) | 골수이식 후 생착 촉진용 조성물 | |
KR20210002205A (ko) | 면역세포 배양액 함유 조성물의 제조방법 및 기능성 화장품 조성물 | |
KR20190138244A (ko) | 편도 유래 중간엽 줄기세포의 조정 배지를 포함하는 이식편대숙주 질환 예방 혹은 치료용 조성물 | |
JP5301994B2 (ja) | Lak細胞の増殖方法 | |
CN111566204A (zh) | 培养细胞的制造方法、脊髓损伤疾病的治疗剂的制造方法 | |
CN108853129B (zh) | 升麻素苷在制备髓源性抑制细胞抑制药物中的应用 | |
JP2020137518A (ja) | 添加剤 | |
US10751371B2 (en) | Use of allogeneic interstitial vessel-layer cell and allogeneic mesenchymal progenitor cell for preventing or treating osteoarthritis | |
US20200155608A1 (en) | Therapeutic Treatments Via Intravenous Infusion Of Mesenchymal Stem Cells | |
WO2014075634A1 (zh) | 间质血管层细胞和间充质祖细胞在预防或治疗骨性关节炎中的应用 | |
WO2018066531A1 (ja) | 抗体産生力を上げる培養上清製剤 | |
WO2014141219A1 (en) | Umbilical cord blood derived stem cell transplantation for the treatment of neural disorder | |
JP7044429B1 (ja) | 腫瘍を縮小、又は消失させるための組成物 | |
WO2023143519A1 (zh) | 一种细胞修复蛋白提取物及其制备方法和其应用 | |
CN110840914B (zh) | 细胞治疗剂用于缓解或改善血管病变的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AA64 | Notification of invalidation of claim of internal priority (with term) |
Free format text: JAPANESE INTERMEDIATE CODE: A241764 Effective date: 20190716 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190722 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190816 |
|
A871 | Explanation of circumstances concerning accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A871 Effective date: 20190828 |
|
A975 | Report on accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A971005 Effective date: 20190911 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20191023 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20191028 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6611400 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |