JP6596498B2 - 流体試料中のナノ粒子を光学的に検出する方法および装置 - Google Patents
流体試料中のナノ粒子を光学的に検出する方法および装置 Download PDFInfo
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Description
試料を照射するために空間的インコヒーレントビームを放出する光源と、
顕微鏡対物レンズを備えた撮像光学システムと、
前記撮像光学システムによる前記顕微鏡対物レンズの物体焦点面と共役の検出面を備え、1ミリ秒より短い予め設定された期間中に、取得すべき前記試料の分析容積の連続した像であって、各像は、前記試料上に入射している前記照射ビームと前記分析容積内に存在する前記各ナノ粒子によって散乱したビームとの光学的干渉によって生じるものである連続した像を取得可能とする二次元光学検出器(40)と、
前記分析容積の各ナノ粒子について前記散乱ビームの前記強度を測定するために、連続した各前記像の平均を取得して、前記平均を各像から引くことを可能とする画像処理手段と、
を備える装置に関する。
前記試料を照射するための空間的インコヒーレント光ビームを放出することと、
顕微鏡対物レンズを備えた撮像光学システムを用いて、二次元光学検出器の検出面(41)上に、前記顕微鏡対物レンズの物体焦点面に近接して配置された前記試料の分析容積の像を形成することと、
前記二次元検出器を用いて前記試料の前記分析容積の連続した像であって、各像は、1ミリ秒より短い予め設定された期間中に前記照射ビームと前記分析容積内に存在する各前記ナノ粒子のそれぞれによって散乱された前記ビームとの各光学干渉によって生じるものである連続した像を取得することと、
前記分析容積の各ナノ粒子について前記散乱したビームの前記強度を測定すべく、連続した前記像の平均をとって前記平均を各像から引くために各前記像を処理することと、
を備える方法に関する。
ここでΦは、光源ビームと散乱ビームとの間での位相シフトである。
ここで、kBはボルツマン定数であり、ηは温度Tでその中にナノ粒子が浸漬されている流体の粘度である。
Claims (6)
- 流体試料の中で動いているナノ粒子を光透過式に検出する装置(100)であって、
試料を照射するために空間的インコヒーレントビームを放出する光源(10)と、
顕微鏡対物レンズ(31)を備えた撮像光学システム(30)と、
前記撮像光学システム(30)による前記顕微鏡対物レンズの物体焦点面と共役の検出面(41)を備え、1ミリ秒より短い予め設定された期間中に、取得すべき前記試料の分析容積の連続した像であって、各像は、前記試料上に入射している前記照射ビームと前記分析容積内に存在する前記各ナノ粒子によって散乱したビームとの光学的干渉によって生じるものである連続した像を取得可能とする二次元光学検出器(40)と、
前記分析容積の各ナノ粒子について前記散乱ビームの前記強度を測定するために、連続した各前記像の平均を取得して、前記平均を各像から引くことを可能とする画像処理手段(50)と、
を備える装置。 - 前記光源はパルス源であり、前記予め設定された期間の各光パルスの前記連続放出を可能にしており、前記装置は前記連続した像の取得のために前記二次元検出器と前記パルス光源とを同期させる手段をさらに備える請求項1に記載の装置。
- 使用している前記顕微鏡対物レンズの開口数は1以上である、請求項1または2に記載の装置。
- 流体試料(20)の中で動いているナノ粒子を光透過式に検出する方法であって、
前記試料(20)を照射するための空間的インコヒーレント光ビームを放出することと、
顕微鏡対物レンズ(31)を備えた撮像光学システム(30)を用いて、二次元光学検出器(40)の検出面(41)上に、前記顕微鏡対物レンズの物体焦点面に近接して配置された前記試料の分析容積の像を形成することと、
前記二次元検出器を用いて前記試料の前記分析容積の連続した像であって、各像は、1ミリ秒より短い予め設定された期間中に前記照射ビームと前記分析容積内に存在する各前記ナノ粒子のそれぞれによって散乱された前記ビームとの各光学干渉によって生じるものである連続した像を取得することと、
前記分析容積の各ナノ粒子について前記散乱したビームの前記強度を測定すべく、連続した前記像の平均をとって前記平均を各像から引くために各前記像を処理することと、
を備える方法。 - 前記光ビームを放出することは、
前記予め設定された期間中に連続して光パルスを放出することと、
前記光パルスの前記放出と同期して前記像を取得することと、
を備える請求項4に記載の方法。 - このような処理による前記連続した像を起点として前記ナノ粒子の前記軌跡を測定することをさらに備える請求項4または5に記載の方法。
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FR1459690A FR3027107B1 (fr) | 2014-10-09 | 2014-10-09 | Methode et dispositif de detection optique de nanoparticules dans un echantillon fluide |
PCT/EP2015/072425 WO2016055306A1 (fr) | 2014-10-09 | 2015-09-29 | Methode et dispositif de detection optique de nanoparticules dans un echantillon fluide |
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FR3027107B1 (fr) * | 2014-10-09 | 2019-09-13 | Espci Paristech | Methode et dispositif de detection optique de nanoparticules dans un echantillon fluide |
US10641696B2 (en) * | 2015-09-18 | 2020-05-05 | New York University | Holographic detection and characterization of large impurity particles in precision slurries |
CN115373127A (zh) * | 2018-01-30 | 2022-11-22 | 瑞巴斯生物系统 | 用于检测目标上的颗粒的方法和系统 |
EP3575773A1 (en) * | 2018-05-28 | 2019-12-04 | Universität für Bodenkultur Wien | A method for determining a three-dimensional particle distribution in a medium |
WO2020054466A1 (ja) * | 2018-09-12 | 2020-03-19 | 国立研究開発法人産業技術総合研究所 | 微粒子観察装置及び微粒子観察方法 |
GB201819033D0 (en) | 2018-11-22 | 2019-01-09 | Cambridge Entpr Ltd | Particle characterization using optical microscopy |
CN109297874B (zh) * | 2018-11-30 | 2023-09-22 | 浙江大学 | 一种用于测量运动颗粒粒径的全息实时测量方法及装置 |
FR3093807B1 (fr) * | 2019-03-13 | 2021-04-16 | Myriade | Dispositif et procédé pour l’observation de microparticules et de nanoparticules. |
US10514331B1 (en) * | 2019-03-23 | 2019-12-24 | Horiba Instruments Incorporated | Method for determining the size of nanoparticles in a colloid |
JP2021076451A (ja) * | 2019-11-07 | 2021-05-20 | ソニー株式会社 | 検出光学系、検出装置、フローサイトメータ及びイメージングサイトメータ |
US10823662B1 (en) * | 2020-02-18 | 2020-11-03 | Horiba Instruments, Incorporated | Special purpose cuvette assembly and method for optical microscopy of nanoparticle colloids |
RU204569U1 (ru) * | 2021-03-23 | 2021-05-31 | Федеральное государственное автономное образовательное учреждение высшего образования "Национальный исследовательский университет "Московский институт электронной техники" | Анализатор траекторий наночастиц в объеме жидкости |
CN113340894B (zh) * | 2021-05-28 | 2023-04-07 | 上海睿钰生物科技有限公司 | 一种非透明微粒的检测方法 |
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FR3027107B1 (fr) * | 2014-10-09 | 2019-09-13 | Espci Paristech | Methode et dispositif de detection optique de nanoparticules dans un echantillon fluide |
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AU2015330241A1 (en) | 2017-05-18 |
EP3204755A1 (fr) | 2017-08-16 |
KR20170091088A (ko) | 2017-08-08 |
JP2017534886A (ja) | 2017-11-24 |
US20170307509A1 (en) | 2017-10-26 |
WO2016055306A1 (fr) | 2016-04-14 |
CA2964079C (en) | 2023-12-19 |
FR3027107B1 (fr) | 2019-09-13 |
CN107003228B (zh) | 2021-05-28 |
AU2015330241B2 (en) | 2021-02-25 |
KR102316439B1 (ko) | 2021-10-22 |
CA2964079A1 (en) | 2016-04-14 |
CN107003228A (zh) | 2017-08-01 |
FR3027107A1 (fr) | 2016-04-15 |
EP3204755B1 (fr) | 2023-06-07 |
US10222319B2 (en) | 2019-03-05 |
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