JP6545676B2 - 改善された代謝能力を有する細菌 - Google Patents

改善された代謝能力を有する細菌 Download PDF

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JP6545676B2
JP6545676B2 JP2016530177A JP2016530177A JP6545676B2 JP 6545676 B2 JP6545676 B2 JP 6545676B2 JP 2016530177 A JP2016530177 A JP 2016530177A JP 2016530177 A JP2016530177 A JP 2016530177A JP 6545676 B2 JP6545676 B2 JP 6545676B2
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strain
modified
gene
parent
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JP2016536993A5 (cg-RX-API-DMAC7.html
JP2016536993A (ja
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フレデリック アール． ブラットナー，
フレデリック アール． ブラットナー，
デイビッド エー． フリッシュ，
デイビッド エー． フリッシュ，
ロバート イー． ノビー，
ロバート イー． ノビー，
テランス エム． ヘンカー，
テランス エム． ヘンカー，
エリック エー． ステフェン，
エリック エー． ステフェン，
ホセ エー． サンチェス，
ホセ エー． サンチェス，
クリストファー アール． ブラットナー，
クリストファー アール． ブラットナー，
ヒュンシク チョイ，
ヒュンシク チョイ，
ジォルジュ ポスファイ，
ジォルジュ ポスファイ，
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スカラブ ゲノミクス， エルエルシー
スカラブ ゲノミクス， エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016530177A 2013-11-14 2014-11-13 改善された代謝能力を有する細菌 Active JP6545676B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361904298P 2013-11-14 2013-11-14
US61/904,298 2013-11-14
PCT/US2014/065545 WO2015073720A1 (en) 2013-11-14 2014-11-13 Bacteria with improved metabolic capacity

Publications (3)

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JP2016536993A JP2016536993A (ja) 2016-12-01
JP2016536993A5 JP2016536993A5 (cg-RX-API-DMAC7.html) 2017-12-28
JP6545676B2 true JP6545676B2 (ja) 2019-07-17

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US (2) US9902965B2 (cg-RX-API-DMAC7.html)
EP (1) EP3068887B1 (cg-RX-API-DMAC7.html)
JP (1) JP6545676B2 (cg-RX-API-DMAC7.html)
WO (1) WO2015073720A1 (cg-RX-API-DMAC7.html)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016242996B2 (en) 2015-04-02 2021-02-18 Scarab Genomics, Llc Materials and methods for extended continuous flow fermentation of reduced genome bacteria
IL316539A (en) 2016-03-15 2024-12-01 Rnaissance Ag Llc Methods and compositions for the enhanced production of double-stranded RNA
MX2020002382A (es) 2017-09-05 2021-01-20 Scarab Genomics Llc Métodos y composiciones para mejorar la expresión de proteínas recombinantes.
CN108587992B (zh) * 2018-04-10 2022-03-15 安徽大学 一种高效分泌表达抗VEGF-Fab抗体片段的高产菌株及其构建方法
BR112021024175A2 (pt) * 2019-05-30 2022-04-12 Rnaissance Ag Llc Métodos e composições aprimorados para aumentar a produção de rna fita dupla
WO2021211862A1 (en) * 2020-04-15 2021-10-21 University of Alaska Anchorage Methods and compositions for the production of isobutene
US12168679B2 (en) 2020-11-10 2024-12-17 Northwestern University Modified bacteria for enhanced bioproduction and methods of using the same
CN120882735A (zh) 2023-01-25 2025-10-31 诺韦尔生物技术美国公司 用于产品制造的表达系统

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US5578464A (en) 1989-10-31 1996-11-26 Schering Corporation E. coli secretory strains
US5747662A (en) 1995-03-01 1998-05-05 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
US6022952A (en) 1998-04-01 2000-02-08 University Of Alberta Compositions and methods for protein secretion
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8119365B2 (en) 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
US7300776B2 (en) * 2004-04-26 2007-11-27 Ajinomoto Co., Inc. L-amino acid-producing bacterium and a method for producing L-amino acid
US20080009041A1 (en) * 2004-11-26 2008-01-10 Hiroshi Mizoguchi Industrially Useful Microorganism
EP1915445A2 (en) 2005-08-20 2008-04-30 Scarab Genomics, LLC Reduced genome e. coli
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
WO2007140816A1 (en) * 2006-06-09 2007-12-13 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
JP5778426B2 (ja) * 2008-02-22 2015-09-16 スカラブ ジェノミクス リミティド ライアビリティ カンパニー アミノ酸生合成のための組成物及び方法
EP2298880A4 (en) * 2008-03-18 2012-02-08 Kyowa Hakko Kirin Co Ltd INDUSTRIALLY USEFUL MICROORGANISM
KR20130101030A (ko) * 2010-08-27 2013-09-12 메타볼릭 익스플로러 변형된 미생물을 사용한 개선된 글리콜산 발효 생산
CA2850842C (en) 2011-10-20 2021-06-08 Scarab Genomics, Llc Reduced genome bacteria with improved genetic stability
CA2940316C (en) * 2014-03-03 2022-03-15 Scarab Genomics, Llc Enhanced production of recombinant crm197 in e. coli

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Publication number Publication date
JP2016536993A (ja) 2016-12-01
WO2015073720A1 (en) 2015-05-21
US9902965B2 (en) 2018-02-27
EP3068887B1 (en) 2019-10-30
US20180208933A1 (en) 2018-07-26
EP3068887A1 (en) 2016-09-21
EP3068887A4 (en) 2017-05-10
US20160298126A1 (en) 2016-10-13

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