MX2020002382A - Métodos y composiciones para mejorar la expresión de proteínas recombinantes. - Google Patents

Métodos y composiciones para mejorar la expresión de proteínas recombinantes.

Info

Publication number
MX2020002382A
MX2020002382A MX2020002382A MX2020002382A MX2020002382A MX 2020002382 A MX2020002382 A MX 2020002382A MX 2020002382 A MX2020002382 A MX 2020002382A MX 2020002382 A MX2020002382 A MX 2020002382A MX 2020002382 A MX2020002382 A MX 2020002382A
Authority
MX
Mexico
Prior art keywords
recombinant proteins
methods
compositions
improved expression
improved
Prior art date
Application number
MX2020002382A
Other languages
English (en)
Inventor
John Brandon
Frederick R Blattner
Robert E Novy
David A Frisch
Charles Landry
Hyunsic Choi
Eric A Steffen
Original Assignee
Scarab Genomics Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scarab Genomics Llc filed Critical Scarab Genomics Llc
Publication of MX2020002382A publication Critical patent/MX2020002382A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En la presente descripción se proporcionan métodos para mejorar la producción de proteínas recombinantes dirigidas al periplasma en cepas huésped de E. coli. Además, se proporcionan cepas huésped de E. coli con capacidad mejorada para producir proteínas recombinantes.
MX2020002382A 2017-09-05 2018-09-04 Métodos y composiciones para mejorar la expresión de proteínas recombinantes. MX2020002382A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762554443P 2017-09-05 2017-09-05
PCT/US2018/049422 WO2019050872A1 (en) 2017-09-05 2018-09-04 METHODS AND COMPOSITIONS FOR ENHANCED EXPRESSION OF RECOMBINANT PROTEINS

Publications (1)

Publication Number Publication Date
MX2020002382A true MX2020002382A (es) 2021-01-20

Family

ID=65634332

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020002382A MX2020002382A (es) 2017-09-05 2018-09-04 Métodos y composiciones para mejorar la expresión de proteínas recombinantes.

Country Status (7)

Country Link
US (2) US11667944B2 (es)
EP (1) EP3679050B1 (es)
JP (2) JP7296386B2 (es)
AU (2) AU2018329671B2 (es)
CA (1) CA3074718A1 (es)
MX (1) MX2020002382A (es)
WO (1) WO2019050872A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2020002382A (es) * 2017-09-05 2021-01-20 Scarab Genomics Llc Métodos y composiciones para mejorar la expresión de proteínas recombinantes.

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8765408B2 (en) 2002-01-23 2014-07-01 Wisconsin Alumni Research Foundation Prophage element-free bacteria
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US8119365B2 (en) 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
EP1915445A2 (en) * 2005-08-20 2008-04-30 Scarab Genomics, LLC Reduced genome e. coli
BRPI0710210A2 (pt) 2006-03-30 2011-05-24 Glaxomithkline Biolog S A composição imunogênica, vacina, métodos para preparar a vacina, e para prevenir ou tratar infecção estafilocócica, uso da composição imunogênica, e, processo para conjugar oligassacarìdeo ou polissacarìdeo capsular
EP2298880A4 (en) * 2008-03-18 2012-02-08 Kyowa Hakko Kirin Co Ltd INDUSTRIALLY USEFUL MICROORGANISM
US20110189743A1 (en) 2009-10-06 2011-08-04 Bio Architecture Lab, Inc. Microbial systems for producing commodity chemicals
GB0917647D0 (en) 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system
US9109212B2 (en) 2012-07-05 2015-08-18 Jiangnan University Method for enhancing extracellular secretion of recombinant proteins in Escherichia coli by co-expressing Thermobifida fusca cutinase
JP6325544B2 (ja) 2012-09-17 2018-05-16 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 原核細胞のペリプラズムにおけるポリペプチドの産生のための方法
WO2015073720A1 (en) 2013-11-14 2015-05-21 Scarab Genomics, Llc Bacteria with improved metabolic capacity
EP3113800B1 (en) 2014-03-03 2024-06-19 Scarab Genomics, LLC Enhanced production of recombinant crm197 in e. coli
US10604736B2 (en) 2015-04-02 2020-03-31 Scarab Genomics Llc Materials and methods for extended continuous flow fermentation of reduced genome bacteria
MX2020002382A (es) * 2017-09-05 2021-01-20 Scarab Genomics Llc Métodos y composiciones para mejorar la expresión de proteínas recombinantes.

Also Published As

Publication number Publication date
EP3679050A4 (en) 2021-06-16
AU2018329671B2 (en) 2023-12-07
US11667944B2 (en) 2023-06-06
CA3074718A1 (en) 2019-03-14
EP3679050B1 (en) 2023-11-22
US20230287472A1 (en) 2023-09-14
US11970725B2 (en) 2024-04-30
AU2018329671A1 (en) 2020-03-12
EP3679050A1 (en) 2020-07-15
JP7296386B2 (ja) 2023-06-22
US20200318150A1 (en) 2020-10-08
JP2020532322A (ja) 2020-11-12
JP2023073442A (ja) 2023-05-25
WO2019050872A1 (en) 2019-03-14
WO2019050872A9 (en) 2019-04-18
AU2024200209B2 (en) 2024-06-06
AU2024200209A1 (en) 2024-02-01

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