JP6534665B2 - 微細気泡発生装置を有する往復動撹拌装置 - Google Patents
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Description
(実施例1)
先ず、動物細胞を培養する培養液3に細胞保護剤(ダイゴGF21)を添加した場合において、既述のスパージャー10(細孔径dが1μmの多孔質体19)から生成する気泡13の粒度分布を測定した。この粒径は、レーザー回折散乱式粒度分布計を用いて、スパージャー10により気泡13を発生させた培養液3をこの粒度分布計内のフローセルに連続的に供給し、この培養液3にレーザー光を照射して当該レーザー光の回折や散乱を評価することによって測定した。
前記の実施例1と同様に、添加剤の添加量と生成する気泡13の粒径との相関関係ついて、添加する添加剤の種類及び添加量を変えて実験を行った。
先ず、既述のように培養液3の表面張力に応じて発生する気泡13の粒径が変化することから、粒径が200μm以下の微小な気泡13を発生させるために必要な培養液3の表面張力がどの程度なのか確認した。具体的には、添加剤としてダイゴGF21を用いると共に、この添加剤の添加量を種々変えた培養液3中において既述のスパージャー10によって気泡13を発生させ、この培養液3の表面張力と発生した気泡13の粒径とを測定した。その結果、図11に示すように、培養液3の表面張力と発生する気泡13の粒径とには直線的な相関関係が見られ、この関係は以下の(1)式により表されることが分かった。
y=28.98x−1292 ・・・(1)
この(1)式から、既述のように200μm以下の微小な粒径の気泡13を発生させるためには、培養液3の表面張力を51.5dyne/cm以下にする必要のあることが分かった。
そこで、以下の表1〜3に示す添加剤について、夫々の濃度を変えて添加した時の培養液3について表面張力を評価した。そして、このような微小な気泡13が生成すると考えられる場合(表面張力が51.5dyne/cm以下)を○とし、これよりも大きな粒径の場合(表面張力が51.5dyne/cmより大きい)には×とした。この結果を以下の表1〜3に示す。
次に、微生物を培養する培地(表面張力:48.6dyne/cm)において、気泡13の気泡径と多孔質体19の細孔径dとの対応関係を測定したところ、図12に示す結果が得られた。この結果に基づいて前記の対応関係を近似する1次式を算出したところ、
y=3.4x+17.5 ・・・(2)
が得られた(x:多孔質体19の細孔径、y:気泡13の粒径(50%径))。この時のR2値は1.0であり、従ってこの(2)式により、培養液3中の気泡13の粒径から多孔質体19の細孔径dを極めて高い精度で算出できることが分かる。そこで、前記の浮力の影響が極めて少ないと考えられる気泡13の粒径(200μm)に対応する多孔質体19の細孔径dを算出すると、50μmとなることが分かった。従って、例えば、細孔径dが50μm以下の多孔質体19を用いることにより、浮力の影響の極めて小さい微細な気泡13を得られることになる。
2 培養容器
2a 頂面部
3 培養液
4 駆動軸
5 開口部
6 支持部
7 往復駆動装置
8 撹拌翼
8a 長径
8b 短径
9 微細気泡発生装置
10 スパージャー
10a 内部領域
11 気体貯留部
12 気体供給流路
13 気泡
14 排気路
15 ニードルバルブ
16 圧力計
17 流量計
18 ボールバルブ
19 多孔質体
20 細孔
21 細胞
Claims (3)
- 被撹拌物が挿入される撹拌容器と、該撹拌容器内に設けられた、軸方向に上下に往復動する駆動軸と、該駆動軸に交叉するように連結固定した撹拌翼と、微細気泡発生装置とよりなり、
該微細気泡発生装置は、多孔質体よりなるスパージャーと、該スパージャーに気体を供給する気体供給手段とよりなり、
前記撹拌翼は、長径と短径よりなる、長方形又は楕円形又は長円形であり、
前記スパージャーは、前記駆動軸の軸方向に延びる管状であり、該スパージャーは、前記撹拌翼の長径の外方に設けられ、
前記気体供給手段により前記スパージャーに供給された気体が、前記多孔質体の細孔を通じて、前記被撹拌物内に気泡が発生されることを特徴とする微細気泡発生装置を有する往復動撹拌装置。 - 前記撹拌翼の振動は、5Hz以下であることを特徴とする請求項1に記載の微細気泡発生装置を有する往復動撹拌装置。
- 前記微細気泡発生装置から1mm以下の径の気泡が発生されるように、前記多孔質体の細孔の径が形成されていることを特徴とする請求項1または2に記載の微細気泡発生装置を有する往復動撹拌装置。
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