JP6430253B2 - 相互作用を検出および測定するための核酸ベースのリンカー - Google Patents

相互作用を検出および測定するための核酸ベースのリンカー Download PDF

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JP6430253B2
JP6430253B2 JP2014540177A JP2014540177A JP6430253B2 JP 6430253 B2 JP6430253 B2 JP 6430253B2 JP 2014540177 A JP2014540177 A JP 2014540177A JP 2014540177 A JP2014540177 A JP 2014540177A JP 6430253 B2 JP6430253 B2 JP 6430253B2
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nucleic acid
binding
binding partner
oligonucleotide
oligonucleotides
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JP2014532430A (ja
JP2014532430A5 (https=
Inventor
フィリップ ウォン，ウェズリー
フィリップ ウォン，ウェズリー
アンダース ハルヴォルセン，ケネス
アンダース ハルヴォルセン，ケネス
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Boston Childrens Hospital
Harvard University
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2014540177A 2011-11-05 2012-11-05 相互作用を検出および測定するための核酸ベースのリンカー Active JP6430253B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161556194P 2011-11-05 2011-11-05
US61/556,194 2011-11-05
US201261666655P 2012-06-29 2012-06-29
US61/666,655 2012-06-29
PCT/US2012/063538 WO2013067489A1 (en) 2011-11-05 2012-11-05 Nucleic acid-based linkers for detecting and measuring interactions

Publications (3)

Publication Number Publication Date
JP2014532430A JP2014532430A (ja) 2014-12-08
JP2014532430A5 JP2014532430A5 (https=) 2015-12-24
JP6430253B2 true JP6430253B2 (ja) 2018-11-28

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JP2014540177A Active JP6430253B2 (ja) 2011-11-05 2012-11-05 相互作用を検出および測定するための核酸ベースのリンカー

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US (2) US9914958B2 (https=)
JP (1) JP6430253B2 (https=)
WO (1) WO2013067489A1 (https=)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013067489A1 (en) * 2011-11-05 2013-05-10 President And Fellows Of Harvard College Nucleic acid-based linkers for detecting and measuring interactions
US10876177B2 (en) 2013-07-10 2020-12-29 President And Fellows Of Harvard College Compositions and methods relating to nucleic acid-protein complexes
WO2015164602A2 (en) * 2014-04-23 2015-10-29 Children's Medical Center Corporation High-throughput structure determination using nucleic acid calipers
WO2016089588A1 (en) * 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
WO2016196824A1 (en) * 2015-06-02 2016-12-08 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
WO2017003950A2 (en) * 2015-06-27 2017-01-05 Children's Medical Center Corporation Compositions and methods for analyte detection using nanoswitches
EP3356547B1 (en) * 2015-10-01 2020-11-18 Dynamic Biosensors GmbH Method for detecting molecular interactions using an immobilized nucleic acid
WO2017139409A1 (en) * 2016-02-09 2017-08-17 Children's Medical Center Corporation Method for detection of analytes via polymer complexes
EP3420352B1 (en) 2016-02-25 2023-08-09 Children's Medical Center Corporation Spinning apparatus for measurement of characteristics relating to molecules
US11414694B2 (en) 2016-03-11 2022-08-16 Children's Medical Center Corporation Nucleic acid nanoswitch catenanes
WO2017165585A1 (en) 2016-03-23 2017-09-28 Children's Medical Center Corporation Systems and apparatus for detecting compounds in human biological samples
US12123869B2 (en) * 2016-03-23 2024-10-22 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
WO2017189794A1 (en) * 2016-04-27 2017-11-02 President And Fellows Of Harvard College Method of secure communication via nucleotide polymers
US10823699B2 (en) 2016-07-29 2020-11-03 Vital Biosciences, Inc. Gel electrophoresis diagnostic kit and methods of using the same
CN115948505B (zh) 2016-08-02 2025-07-25 哈佛学院院长及董事 交叉协同自组装体
US12252507B2 (en) 2016-12-05 2025-03-18 Children's Medical Center Corporation Nucleic acid nanoswitch construction methods
CA3048210A1 (en) 2016-12-23 2018-06-28 Vital Biosciences, Inc. Electrophoresis diagnostic methods and kits
WO2019100080A1 (en) 2017-11-20 2019-05-23 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
FR3075820B1 (fr) 2017-12-21 2022-12-30 Paris Sciences Lettres Quartier Latin Molecule d'adn double-brin pour la detection et la caracterisation des interactions moleculaires
US11561221B2 (en) * 2018-05-02 2023-01-24 Trustees Of Boston University Dynamic tracking of captured targets for enhanced digital biosensing
JP7537748B2 (ja) 2018-06-06 2024-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
JP7549587B2 (ja) * 2019-02-04 2024-09-11 アコヤ・バイオサイエンシズ・インコーポレイテッド 生物学的試料の選択的標識による分析物検出
US12571802B2 (en) 2020-02-29 2026-03-10 President And Fellows Of Harvard College Nanoswitch caliper trains for high-throughput, high-resolution structural analysis of biomolecules
CN111549098B (zh) * 2020-06-16 2024-10-29 南开大学 测量人端粒精准长度的单分子力学方法
CN111721504B (zh) * 2020-06-17 2022-02-18 中国科学院物理研究所 在小力下测算磁镊高度转换系数的方法及应用
US20230407374A1 (en) * 2020-11-09 2023-12-21 Children's Medical Center Corporation Systems and methods for high throughput screening of molecular interactions
CN119563035A (zh) 2022-04-01 2025-03-04 德皮克萨斯股份有限公司 筛选化合物的方法
WO2025041065A1 (en) * 2023-08-21 2025-02-27 Depixus SAS Device for determining molecular interactions

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9114525D0 (en) 1991-07-05 1991-08-21 Delnatte Sabine Homogeneous nucleic acids probe based tests and substances
JPH09500378A (ja) 1993-07-02 1997-01-14 リンクス セラピューティクス，インコーポレイティド 枝分れされそして複雑に結合された高分子構造体の集中的合成
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
DE69719057T2 (de) * 1996-07-12 2003-12-04 Tm Technologies Inc., Woburn Verfahren zur signalverstärkung
EP1034040A2 (en) 1997-11-25 2000-09-13 Mosaic Technologies Devices and methods for detecting target molecules in biological samples
US20020177144A1 (en) 1997-12-30 2002-11-28 Jose Remacle Detection and/or quantification method of a target molecule by a binding with a capture molecule fixed on the surface of a disc
US6143504A (en) * 1998-10-27 2000-11-07 Arch Development Corporation Methods and compositions for the diagnosis of fragile X syndrome
GB9827912D0 (en) * 1998-12-19 1999-02-10 Univ Manchester Detecting nucleic acids
JP3080951B1 (ja) * 1999-03-04 2000-08-28 株式会社分子バイオホトニクス研究所 ウイルスを検出する方法
EP2184369A1 (en) 2001-07-11 2010-05-12 Applied Biosystems, LLC Method for normalisation in electrophoresis
US20030143549A1 (en) 2002-01-29 2003-07-31 Eastman Kodak Company Method for DNA sequencing and gene identification
US7294513B2 (en) 2002-07-24 2007-11-13 Wyatt Technology Corporation Method and apparatus for characterizing solutions of small particles
AU2003263937B2 (en) * 2002-08-19 2010-04-01 The President And Fellows Of Harvard College Evolving new molecular function
AU2002952797A0 (en) 2002-11-20 2002-12-05 Bio-Molecular Holdings Pty Limited Centrifugal device and method using same
EP2365095A1 (en) * 2003-02-26 2011-09-14 Callida Genomics, Inc. Random array DNA analysis by hybridization
WO2006041194A1 (ja) 2004-10-15 2006-04-20 Japan Science And Technology Agency ｍＲＮＡ－ピューロマイシン－タンパク質連結体作製用リンカー
US20070026423A1 (en) * 2005-03-16 2007-02-01 Thomas Koehler Method and test kit for the detection of target nucleic acids
KR20080028886A (ko) * 2005-05-26 2008-04-02 앙상블 디스커버리 코포레이션 핵산-템플레이팅된 화학 반응에 의한 생검출
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
US20100035247A1 (en) 2005-11-04 2010-02-11 U.S. Genomics, Inc. Heterogeneous Assay of Analytes in Solution Using Polymers
EP1963531B1 (en) * 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2008054834A2 (en) * 2006-02-03 2008-05-08 Columbia University Binary deoxyribozyme probes for nucleic acid analysis
US8685894B2 (en) * 2006-05-25 2014-04-01 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University Modified nucleic acid nanoarrays and uses therefor
US20090286694A1 (en) 2006-08-21 2009-11-19 Gafur Zainiev Nucleic acid array with releaseable nucleic acid probes
JP2008259453A (ja) * 2007-04-12 2008-10-30 Toyobo Co Ltd 核酸の検出方法
US8043810B2 (en) 2007-05-02 2011-10-25 Eagle Eye Research, Inc. Analyte detection using autocatalytic chain reactions
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
CN102271712B (zh) 2008-10-31 2015-11-25 通用医疗公司 用于将物质递送至生物靶标的组合物和方法
US9354189B2 (en) 2008-12-02 2016-05-31 President And Fellows Of Harvard College Apparatus for measurement of spinning forces relating to molecules
US8491454B2 (en) 2008-12-02 2013-07-23 President And Fellows Of Harvard College Spinning force apparatus
EP2452195A4 (en) 2009-07-07 2012-12-05 Agency Science Tech & Res METHODS OF IDENTIFYING A PAIR OF LIAISON PARTNERS
WO2011047087A2 (en) * 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
US9138418B2 (en) 2009-12-09 2015-09-22 William Marsh Rice University Therapeutic compositions and methods for delivery of active agents cleavably linked to nanoparticles
WO2012044387A2 (en) 2010-07-06 2012-04-05 T2 Biosystems, Inc. Methods and compositions for detection of analytes
WO2013067489A1 (en) * 2011-11-05 2013-05-10 President And Fellows Of Harvard College Nucleic acid-based linkers for detecting and measuring interactions
US9182334B2 (en) 2012-02-24 2015-11-10 Agilent Technologies, Inc. Method of determining thermodynamic and kinetic parameters from measured off rates
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
WO2013177046A1 (en) 2012-05-21 2013-11-28 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
WO2014011800A1 (en) 2012-07-10 2014-01-16 Pivot Bio, Inc. Methods for multipart, modular and scarless assembly of dna molecules
US10876177B2 (en) 2013-07-10 2020-12-29 President And Fellows Of Harvard College Compositions and methods relating to nucleic acid-protein complexes
US9333463B2 (en) 2013-07-26 2016-05-10 General Electric Company Devices and systems for elution of biomolecules
US9322051B2 (en) 2013-10-07 2016-04-26 General Electric Company Probing of biological samples
US20150361422A1 (en) 2014-06-16 2015-12-17 Agilent Technologies, Inc. High throughput gene assembly in droplets
WO2016089588A1 (en) 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
WO2017003950A2 (en) 2015-06-27 2017-01-05 Children's Medical Center Corporation Compositions and methods for analyte detection using nanoswitches

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Publication number Publication date
US20180291434A1 (en) 2018-10-11
JP2014532430A (ja) 2014-12-08
US20140255939A1 (en) 2014-09-11
WO2013067489A1 (en) 2013-05-10
WO2013067489A9 (en) 2013-09-12
US9914958B2 (en) 2018-03-13

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