JP6412866B2 - 有機ターゲットに結合するための反復断片を持たないクモ糸融合タンパク質の構造 - Google Patents

有機ターゲットに結合するための反復断片を持たないクモ糸融合タンパク質の構造 Download PDF

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JP6412866B2
JP6412866B2 JP2015509441A JP2015509441A JP6412866B2 JP 6412866 B2 JP6412866 B2 JP 6412866B2 JP 2015509441 A JP2015509441 A JP 2015509441A JP 2015509441 A JP2015509441 A JP 2015509441A JP 6412866 B2 JP6412866 B2 JP 6412866B2
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protein
fusion protein
amino acid
organic target
moiety
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JP2015515969A (ja
JP2015515969A5 (cg-RX-API-DMAC7.html
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ミュー ヘドハンマル，
ミュー ヘドハンマル，
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Spiber Technologies AB
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Spiber Technologies AB
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1271—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Micrococcaceae (F), e.g. Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T442/00—Fabric [woven, knitted, or nonwoven textile or cloth, etc.]
    • Y10T442/10—Scrim [e.g., open net or mesh, gauze, loose or open weave or knit, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2015509441A 2012-05-02 2013-05-02 有機ターゲットに結合するための反復断片を持たないクモ糸融合タンパク質の構造 Active JP6412866B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12166393 2012-05-02
EP12166393.4 2012-05-02
PCT/EP2013/059146 WO2013164405A1 (en) 2012-05-02 2013-05-02 Spider silk fusion protein structures without repetitive fragment for binding to an organic target

Publications (3)

Publication Number Publication Date
JP2015515969A JP2015515969A (ja) 2015-06-04
JP2015515969A5 JP2015515969A5 (cg-RX-API-DMAC7.html) 2016-06-23
JP6412866B2 true JP6412866B2 (ja) 2018-10-24

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JP2015509441A Active JP6412866B2 (ja) 2012-05-02 2013-05-02 有機ターゲットに結合するための反復断片を持たないクモ糸融合タンパク質の構造

Country Status (7)

Country Link
US (1) US9708376B2 (cg-RX-API-DMAC7.html)
EP (1) EP2844360B1 (cg-RX-API-DMAC7.html)
JP (1) JP6412866B2 (cg-RX-API-DMAC7.html)
CN (1) CN104394953B (cg-RX-API-DMAC7.html)
AU (1) AU2013255815B2 (cg-RX-API-DMAC7.html)
CA (1) CA2872056A1 (cg-RX-API-DMAC7.html)
WO (1) WO2013164405A1 (cg-RX-API-DMAC7.html)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101657298B1 (ko) * 2016-02-03 2016-09-13 (주)넥스젠바이오텍 세포 증식 효과가 증가한 인간 상피세포재생인자 융합단백질 및 이를 유효성분으로 함유하는 피부 주름 및 탄력 개선용 화장료 조성물
KR102790669B1 (ko) 2016-02-11 2025-04-04 시비스 머테리얼 사이언스 리미티드 합성 드래그라인 거미 실크를 포함하는 복합체
EP3263593A1 (en) * 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
US11597750B2 (en) * 2017-06-13 2023-03-07 Aalto University Foundation Sr Method for producing a condensed adhesive phase of silk fusion proteins
WO2020183465A1 (en) * 2019-03-11 2020-09-17 Seevix Material Sciences Ltd. Compositions comprising dragline spider silk
CN113924320A (zh) * 2019-04-02 2022-01-11 邦德威尔科技有限合伙公司 用于增强抗体纯化的功能化ubx蛋白材料
BR112022020937A2 (pt) 2020-04-23 2022-12-20 Seevix Mat Sciences Ltd Composições cosméticas compreendendo seda de aranha dragon sintética.

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6268169B1 (en) * 1993-06-15 2001-07-31 E. I. Du Pont De Nemours And Company Recombinantly produced spider silk
EP0704532B1 (en) 1994-06-10 2002-09-11 United States Surgical Corporation Recombinant chimeric proteins and methods of use thereof
JP2000125872A (ja) 1998-09-07 2000-05-09 Terumo Corp 三量体キメラタンパク質およびキメラタンパク質を含有するコラーゲンマトリックス
DE19932688B4 (de) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
US20050261479A1 (en) 2004-04-29 2005-11-24 Christian Hoffmann Method for purifying and recovering silk proteins using magnetic affinity separation
WO2006076711A2 (en) 2005-01-14 2006-07-20 Trustees Of Tufts College Fibrous protein fusions and use thereof in the formation of advanced organic/inorganic composite materials
RU2444527C2 (ru) * 2005-12-30 2012-03-10 Спибер Текнолоджиз Аб Рекомбинантный спидроиновый белок главной ампулярной железы, способный к полимеризации, рекомбинантный слитый белок, способный к управляемой полимеризации, полимер спидроинового белка главной ампулярной железы, композиция для управляемой полимеризации, рекомбинантная молекула нуклеиновой кислоты (варианты), способ получения растворимого слитого белка, способ получения полимера спидроинового белка главной ампулярной железы и способ культивирования эукариотических клеток
EP1852470A1 (en) 2006-05-03 2007-11-07 Technische Universität München Multilayer Silk Protein Films
EP2401298A1 (en) 2009-02-24 2012-01-04 Glaxo Group Limited Antigen-binding constructs
DK2421890T3 (en) * 2009-04-22 2015-11-23 Spiber Technologies Ab A process for the preparation of polymers of spider silk proteins
JP5726282B2 (ja) * 2010-03-18 2015-05-27 スパイバー テクノロジーズ アーベーＳｐｉｂｅｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ａｂ タンパク質及びポリペプチドの産生
CN102946914B (zh) * 2010-04-12 2015-06-03 思百博技术股份公司 方法及组合物

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Publication number Publication date
US20150087046A1 (en) 2015-03-26
CA2872056A1 (en) 2013-11-07
CN104394953B (zh) 2018-01-23
AU2013255815B2 (en) 2018-03-29
EP2844360A1 (en) 2015-03-11
JP2015515969A (ja) 2015-06-04
CN104394953A (zh) 2015-03-04
US9708376B2 (en) 2017-07-18
WO2013164405A1 (en) 2013-11-07
AU2013255815A1 (en) 2014-11-13
EP2844360B1 (en) 2018-12-19

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