JP6403465B2 - 細菌の固定化方法、細菌固定化担体、及び細菌の保存方法 - Google Patents
細菌の固定化方法、細菌固定化担体、及び細菌の保存方法 Download PDFInfo
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- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
<鉄酸化細菌の固定化>
(1)鉄酸化細菌の菌体の調製
アシディチオバチルスフェロオキシダンス(Licanantay DSM 17318株)を、培養液(9K液体培地)に接種し、30℃において1日間振とう培養した。菌体を遠心分離(10000rpm×10分)で回収した後、硫酸でpH=3.0に調整した0.1Mのβ−アラニン緩衝液を加え、3回洗浄し、得られた湿潤状態のペレット状の洗浄菌体を得た。
0.2質量%アルギン酸ナトリウム(和光純薬社製)水溶液に、0.1〜0.3乾燥質量%になるように、上記ペレット状の菌体を分散させた(図1(a)参照)。更に、鉄微粒子(平均粒子径0.5μm)をアルギン酸ナトリウムの質量に対して1質量%になるように分散させ、分散液を作製した。分散液を自然乾燥(温度30℃、湿度65%RH、1.0気圧)で乾燥させ、水分2.0質量%の細菌固定化担体を得た。図2に得られた細菌固定化担体の走査型電子顕微鏡写真を示す。図2の白矢印が鉄酸化細菌を指し、黒矢印が鉄微粒子を指す。
<菌体添加量が鉄酸化細菌の鉄酸化能力に及ぼす影響>
(1)細菌固定化担体の作製
菌体添加量を0.02〜0.7乾燥質量%になるように菌体を分散させた他は、実施例1と同様に細菌固定化菌体を作製した。
上記で調製した細菌固定化菌体を粉砕し、1mmol/10mlのFeSO4溶液に各0.1mg/mlになるように添加し、Fe2+イオン濃度を経時的に測定し、鉄酸化能力を比較した。結果を図3に示す。
Claims (7)
- アルギン酸ナトリウム水溶液に、硫黄酸化細菌及び/又は鉄酸化細菌を0.01〜1乾燥質量%になるように分散させ、分散液を得る工程、及び
前記分散液を自然乾燥し、細菌固定化担体を得る工程
を含み、
前記分散液を得る工程において、前記細菌と共に磁性体微粒子を分散させる工程を含む細菌の固定化方法。 - 前記アルギン酸ナトリウム水溶液が、0.1〜1.0質量%のアルギン酸ナトリウム水溶液である請求項1に記載の方法。
- 前記自然乾燥工程が、4〜30℃の温度、0〜70%RHの相対湿度の環境下で常圧乾燥又は減圧乾燥する工程である請求項1又は2に記載の方法。
- 前記自然乾燥工程が、前記細菌固定化担体の水分が、0.05〜5.0質量%になるまで乾燥する工程である請求項1〜3のいずれか1項に記載の方法。
- 前記磁性体微粒子が、鉄微粒子、Fe3O4微粒子、ニッケル微粒子、及びコバルト微粒子からなる群から選択される磁性体微粒子である請求項1〜4のいずれか1項に記載の方法。
- 前記磁性体微粒子を、アルギン酸ナトリウムの質量に対して、0.05〜1質量%で分散させる請求項1〜5のいずれか1項に記載の方法。
- 請求項1〜6のいずれか1項に記載の固定化方法により得られた細菌固定化担体を、冷蔵保存することを特徴とする細菌の保存方法。
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