JP6399558B2 - 軟骨細胞から多能性幹細胞を製造する方法 - Google Patents

軟骨細胞から多能性幹細胞を製造する方法 Download PDF

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JP6399558B2
JP6399558B2 JP2015539924A JP2015539924A JP6399558B2 JP 6399558 B2 JP6399558 B2 JP 6399558B2 JP 2015539924 A JP2015539924 A JP 2015539924A JP 2015539924 A JP2015539924 A JP 2015539924A JP 6399558 B2 JP6399558 B2 JP 6399558B2
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cells
population
cartilage
pluripotent stem
polypeptide
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JP2016502401A5 (enExample
JP2016502401A (ja
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ディリーマ,ダリル,ディー.
オリー,ツァイウェイ
コールウェル,クリフォード,ダブリュ.
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スクリップス ヘルス
スクリップス ヘルス
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JP2015539924A 2012-10-29 2013-10-29 軟骨細胞から多能性幹細胞を製造する方法 Active JP6399558B2 (ja)

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Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2900808B1 (en) 2012-09-28 2019-04-03 Scripps Health Methods of differentiating stem cells into chondrocytes
EP2912166B1 (en) 2012-10-29 2019-05-01 Scripps Health Methods of producing pluripotent stem cells from chondrocytes
US9974885B2 (en) 2012-10-29 2018-05-22 Scripps Health Methods of transplanting chondrocytes
US9668892B2 (en) 2013-03-11 2017-06-06 Endospan Ltd. Multi-component stent-graft system for aortic dissections
US10758574B2 (en) * 2016-04-28 2020-09-01 The Board Of Trustees Of The Leland Stanford Junior University Cartilage cells with a high regenerative potential and low immune response for use in cellular therapy
CN105833275B (zh) * 2016-04-28 2019-12-27 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种利用重编程效应治疗白血病的方法
CN106267363B (zh) * 2016-09-18 2019-06-14 辽宁聚高智造科技有限公司 一种人造骨
WO2020130147A1 (ja) 2018-12-21 2020-06-25 国立大学法人京都大学 ルブリシン局在軟骨様組織、その製造方法及びそれを含む関節軟骨損傷治療用組成物
WO2021100796A1 (ja) * 2019-11-20 2021-05-27 一丸ファルコス株式会社 軟骨を作製するための剤及び軟骨の作製方法
JP7774794B2 (ja) * 2021-06-17 2025-11-25 学校法人近畿大学 人工耳介および人工軟骨組織の骨格構造

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5399347A (en) 1987-06-24 1995-03-21 Autoimmune, Inc. Method of treating rheumatoid arthritis with type II collagen
US5645851A (en) 1994-02-28 1997-07-08 Moore; Eugene R. Product for alleviating the symptons of arthritis in mammals
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
GB9503492D0 (en) 1995-02-22 1995-04-12 Ed Geistlich S Hne A G F R Che Chemical product
US6025327A (en) 1997-08-08 2000-02-15 Biocell Technology, Llc Hydrolyzed collagen type II and use thereof
US6511958B1 (en) 1997-08-14 2003-01-28 Sulzer Biologics, Inc. Compositions for regeneration and repair of cartilage lesions
US20030026786A1 (en) 1999-06-04 2003-02-06 Mark F. Pittenger Chondrogenic differentiation of human mesenchymal stem cells
EP1254670A1 (en) 2001-05-03 2002-11-06 Stichting Katholieke Universiteit Type II collagen matrices for use in cartilage engineering
US6780841B2 (en) 2001-11-13 2004-08-24 Biocell Technology, Llc Hyaluronic acid and chondroitin sulfate based hydrolyzed collagen type II and method of making same
CA2412012C (en) 2001-11-20 2011-08-02 Ed. Geistlich Soehne Ag Fuer Chemische Industrie Resorbable extracellular matrix containing collagen i and collagen ii for reconstruction of cartilage
US6852331B2 (en) 2002-02-11 2005-02-08 Taipei Biotechnology Ltd., Inc. Fabrication of a cartilage implant
ES2265199B1 (es) 2003-06-12 2008-02-01 Cellerix, S.L. Celulas madre adultas multipotentes procedentes de condrocitos desdiferenciados y sus aplicaciones.
WO2006015209A2 (en) 2004-07-29 2006-02-09 Stem Cell Innovations, Inc. Differentiation of stem cells
CN101103101B (zh) 2004-09-24 2012-10-10 成血管细胞系统公司 增强间充质前体细胞(mpc)的增生和/或存活的方法
TR201900968T4 (tr) 2004-09-24 2019-02-21 Mesoblast Inc Multipotansiyel genleşmiş mezenkimal prekürsör hücre soyu (memp) ve bunların kullanımı.
US20060189840A1 (en) 2005-02-18 2006-08-24 Acorn Cardiovascular, Inc. Transmyocardial delivery of cardiac wall tension relief
ES2329636B2 (es) * 2006-02-17 2010-07-26 Universitat De Valencia, Estudi General (Participa Con El 70%) Uso del factor pedf para inducir la auto-renovacion de celulas madre.
FR2900155B1 (fr) 2006-04-21 2008-06-27 Diana Naturals Sa Hydrolisat de cartilage aviaire, procede d'obtention et utilisations
CN101721349B (zh) 2008-10-16 2011-07-20 常州百瑞吉生物医药有限公司 可注射原位交联水凝胶及其制备方法和用途
KR101091028B1 (ko) 2009-07-02 2011-12-09 아주대학교산학협력단 체내 주입형 하이드로젤 및 이의 생의학적 용도
JP5636174B2 (ja) 2009-07-10 2014-12-03 学校法人近畿大学 間葉系細胞または軟骨細胞の製法ならびに発癌性の抑制方法
MX389666B (es) * 2009-10-16 2025-03-19 Scripps Research Inst Induccion de celulas pluripotentes.
JP6263329B2 (ja) 2009-11-24 2018-01-17 ザ ユニバーシティ オブ コネチカット ヒト胚性および人工多能性幹細胞の分化
KR100990436B1 (ko) 2009-11-30 2010-10-29 동국대학교 산학협력단 중간엽 줄기세포를 DKK-1 또는 sFRP-1을 이용하여 연골세포로 분화시키는 방법
EP2529009A4 (en) 2010-01-28 2013-11-13 Univ Queensland METHOD OF STEM CELL DIFFERENTIATION
CA2800498C (en) 2010-03-31 2021-11-16 The Scripps Research Institute Reprogramming cells
EP2556147B1 (en) 2010-04-08 2018-10-03 The University Court Of The University of Edinburgh Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof
CN101810855B (zh) 2010-05-20 2012-06-13 广州创尔生物技术有限公司 一种ⅱ型胶原关节软骨修复液及其制备方法
CA2843050A1 (en) 2010-07-26 2012-02-02 The University Of Manchester Targeted differentiation of stem cells
CN101934092A (zh) 2010-08-27 2011-01-05 中国人民解放军第三军医大学第一附属医院 用于软骨再生修复的注射型软骨仿生基质材料及使用方法
WO2012048093A2 (en) 2010-10-08 2012-04-12 Osiris Therapeutics, Inc. Enhanced msc preparations
KR101340458B1 (ko) 2010-11-02 2013-12-11 서울대학교산학협력단 하이드로겔을 포함하는 연골 이식용 조성물
US8663675B2 (en) 2010-11-18 2014-03-04 Jalaledin Ghanavi Injectable matrix having a polymer and a stem cell niche composed of cup-shaped nanoparticles containing growth factors or physiological agents for organ reconstruction
JP2014509517A (ja) 2011-03-18 2014-04-21 アンセルム(アンスチチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル) 軟骨細胞分化培地および細胞の軟骨細胞分化を誘導するための方法
EP2900808B1 (en) 2012-09-28 2019-04-03 Scripps Health Methods of differentiating stem cells into chondrocytes
EP2912166B1 (en) * 2012-10-29 2019-05-01 Scripps Health Methods of producing pluripotent stem cells from chondrocytes
US9974885B2 (en) 2012-10-29 2018-05-22 Scripps Health Methods of transplanting chondrocytes
JP6473686B2 (ja) * 2013-03-25 2019-02-20 公益財団法人神戸医療産業都市推進機構 細胞の選別方法

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JP6898657B2 (ja) 2021-07-07
US20150275181A1 (en) 2015-10-01
EP2912166A4 (en) 2016-06-01
JP2023068668A (ja) 2023-05-17
EP2912166B1 (en) 2019-05-01
WO2014070797A1 (en) 2014-05-08
JP2018183141A (ja) 2018-11-22
EP2912166A1 (en) 2015-09-02
US10385318B2 (en) 2019-08-20
JP2021153591A (ja) 2021-10-07
JP2016502401A (ja) 2016-01-28
US20180155694A1 (en) 2018-06-07
US9914911B2 (en) 2018-03-13

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