JP6350978B2 - High concentration orotic acid solution and method for producing orotic acid-containing beverage - Google Patents

Description

  The present invention relates to a method for producing a high-concentration orotic acid solution or a beverage containing orotic acid, and more specifically, a high-concentration orotic acid having excellent solubility and storage stability of orotic acid even if it contains a high concentration of orotic acid. The present invention relates to a solution and a method for producing an orotic acid-containing beverage using such a high-concentration orotic acid solution.

  Orotic acid is a heteroaromatic ring compound, also called orotic acid, orotic acid, uracil-6-carboxylic acid, and is a precursor of pyrimidine nucleotide synthesis that can be biosynthesized in many higher animals including humans. Milk, powdered milk, whey, etc. Present in dairy products. It is known that orotic acid has various actions effective for maintaining and promoting health, such as a uric acid level lowering action, endurance improvement (reduction of oxygen consumption), and nourishing tonic.

  In order to ingest orotic acid efficiently and simply, it is advantageous to blend it in beverages that are ingested daily such as mineral water and tea at a high concentration. However, orotic acid itself has a problem that its solubility in water is low, and the solubility is lowered by other components such as minerals. A solution containing orotic acid at a concentration of about 0.05% by mass or more. It was difficult to prepare. Further, even if orotic acid is once dissolved in water, it has a very low solubility, so that there is a problem that precipitation or precipitation is likely to occur during storage after dissolution. Therefore, the low solubility and storage stability of orotic acid as described above have been a major obstacle when blending orotic acid in beverages. Furthermore, although the solubility was improved by using a salt such as choline salt of orotic acid, the flavor was not good. Therefore, there has been a demand for an orotic acid-containing beverage in which orotic acid is easily dissolved even when orotic acid is blended at a high concentration, does not precipitate or precipitate during storage, and does not deteriorate in flavor.

  As a method for improving the solubility and storage stability of orotic acid, a method using a basic amino acid or a basic peptide has been proposed (see, for example, Patent Document 1), but basic amino acids such as lysine, arginine and histidine are used. It is also well known to exhibit bitterness (see, for example, Patent Document 2). For beverages that do not like the addition of basic amino acids and basic peptides that have a problem in taste, there is a need for another method that does not use these.

  Carnitine, on the other hand, is a vitamin-like substance involved in lipid metabolism in the body that plays an essential role in transporting fatty acids into the mitochondria, which is the place of combustion, when burning lipids in the body to produce energy. A derivative of an amino acid having a quaternary ammonium in the molecular structure and a betaine structure, and having a methylene group between the bond between the asymmetric carbon that arranges the hydroxyl group, the quaternary ammonium ion, and the carboxylate anion. Carnitine is a substance that does not fall under the definition of structurally defined amino acids as well as α-amino acids constituting proteins.

JP 2011-125282 A JP 2003-274896 A

  The problem of the present invention is that even if it contains orotic acid at a high concentration, it can be easily dissolved even if it contains a high concentration orotic acid solution with excellent solubility and storage stability, It is an object of the present invention to provide a method for producing an orotic acid-containing beverage which is dissolved in the solution, has no precipitation or precipitation during storage, and does not impair the original taste and flavor of the beverage.

  The present inventors previously added orotic acid and basic amino acids such as lysine to beverage ingredients, so that even when orotic acid is blended at a high concentration, orotic acid is easily dissolved and precipitated during storage. Has proposed orotic acid-containing beverages that are free from precipitation (see Patent Document 1), but basic amino acids such as lysine exhibit bitterness, and the basic amino acids and orotic acids are added and dissolved individually in the beverage ingredients. There was a limit to increasing the orotic acid concentration. Accordingly, various studies were made on a solubilizing agent capable of preparing a high concentration orotic acid solution of 5 to 15% by mass, and it was found that when carnitine was excessively added to orotic acid, a high concentration orotic acid solution could be prepared. . In addition, even when such a high concentration orotic acid solution is blended in a beverage ingredient, orotic acid can be easily dissolved, and there is no precipitation or precipitation during storage, and an orotic acid-containing beverage that does not impair the original taste and flavor of the beverage. The present invention has been completed by finding out that it can be obtained.

That is, the present invention is as follows.
(1) containing orotic acid in a range of 0.5 to 20% by mass and carnitine in a range of 0.6 to 50% by mass, and orotic acid and carnitine in a range of 1: 1.2 to 1:10 by mass ratio When the orotic acid concentration in the high-concentration orotic acid solution is 0.5 mass% or more and 1.0 mass% or less, carnitine is 1.2 times or more of the orotic acid in a mass ratio, and the orotic acid concentration is 1 When the concentration is higher than 0.0% by mass and within 2.0% by mass, carnitine is 1.5 times or more of the orotic acid in a mass ratio, and the orotic acid concentration is higher than 2.0% by mass. In the case of 0 mass% or less, carnitine is 2.0 times or more of the orotic acid in a mass ratio, and in the case where the orotic acid concentration is higher than 10.0 mass% and within 20.0 mass%, the carnitine is mass It is contained in the range of 2.5 times or more of the orotic acid in a ratio, High concentrations orotic acid solution having improved solubility and storage stability of the acid.
(2) The high-concentration orotic acid solution according to (1) above , wherein sterilization is performed by heat treatment.
(3) In the preparation of the high-concentration orotic acid solution according to (1) or (2), the orotic acid-containing beverage is added at a ratio of 0.05 to 10 parts by mass with respect to 100 parts by mass of the beverage. Manufacturing method.

  According to the present invention, even when orotic acid is contained at a high concentration, orotic acid is easily dissolved even if it is mixed with a high concentration orotic acid solution having excellent solubility and storage stability of orotic acid, or orotic acid at a high concentration. It is possible to provide an orotic acid-containing beverage that dissolves and does not precipitate or precipitate during storage and that does not impair the original taste and flavor of the beverage.

It is a photograph which shows the result investigated about whether the drink containing orotic acid and carnitine produces a Maillard reaction.

  The high-concentration orotic acid solution of the present invention contains 1.2 to 25 mass% orotic acid and 1.44 to 80 mass% carnitine, and 1: 1 mass ratio of orotic acid and carnitine. The solution is not particularly limited as long as it is a solution containing 2 to 1:10, and carnitine improves the solubility of orotic acid, improves the storage stability after dissolution, and has an effect of improving flavor.

  As a method for producing a high-concentration orotic acid solution according to the present invention, orotic acid and carnitine can be dissolved simultaneously to obtain a high-concentration orotic acid solution. However, orotic acid is dissolved in a high-concentration carnitine solution and high-concentration orotic acid solution is prepared. An acid solution is preferred. As the solvent, water such as distilled water or deionized water is usually used, but in some cases, a dilute alcohol aqueous solution can be used.

  In the present invention, orotic acid is uracil-6-carboxylic acid, and any of commercially available products, those obtained by chemical synthesis, those derived from microorganisms, those extracted from foods such as whey, etc. may be used. it can. Examples of orotic acids derived from microorganisms include orotic acids obtained by the production method described in US Pat. No. 5,013,656. The orotic acid in the present invention includes all orotic acid free forms (free forms), orotic acid hydrates, orotic acid derivatives, and pharmacologically acceptable salts thereof. The free form (free form) and hydrates thereof are preferred.

In the present invention, carnitine is 4-trimethylamino-3-hydroxybutyric acid, and any of commercially available products, those obtained by chemical synthesis, those derived from microorganisms, those extracted from milk, etc. can be used. Can do. Examples of carnitine obtained by chemical synthesis include carnitine obtained by the production method described in WO2008 / 056827. Carnitine has D-form and L-form as stereoisomers, and DL-form is known as a mixture. In the present invention, any of L-carnitine, D-carnitine, and a mixture of L-carnitine and D-carnitine can be used. In particular, L-carnitine or a mixture of L-carnitine and D-carnitine is used. Is preferred. The carnitine of the present invention includes carnitine salts such as hydrochloride, acetate and sulfate, but does not include carnitine derivatives such as carnitine acyl ester.

  As described above, L-carnitine acts as a carrier that transports fatty acids into mitochondria in the fatty acid degradation system in the living body, and L-carnitine is a high-energy compound that is generated through the degradation of fat and lipolysis in the living body. As such, L-carnitine is known to have an effect of preventing obesity and an effect of increasing endurance during exercise. For this reason, obesity-preventing effects can also be expected for beverages containing carnitine.

  The orotic acid concentration in the high-concentration orotic acid solution of the present invention is preferably 1.5% by mass as the lower limit, more preferably 2% by mass, in terms of producing a high-concentration orotic acid-containing beverage. % Is particularly preferable, and the upper limit is preferably 25% by mass, more preferably 20% by mass, and particularly preferably 15% by mass. Thus, as the orotic acid concentration, for example, 1.5 to 25% by mass is preferable, 2 to 20% by mass is more preferable, and 2.5 to 15% by mass is particularly preferable. The carnitine concentration in the high-concentration orotic acid solution of the present invention is preferably 2 to 70% by mass, more preferably 10 to 60% by mass, and particularly preferably 20 to 40% by mass in terms of improving the solubility of orotic acid. preferable.

  The mass ratio of orotic acid and carnitine in the high-concentration orotic acid solution of the present invention is preferably 1: 1.5 to 1: 8, more preferably 1: 2 to 1: 5 in terms of improving the solubility of orotic acid. Preferably, 1: 3 to 1: 4 are particularly preferable. If the mass ratio is less than 1: 1.2, the solubility of orotic acid is poor and a high-concentration orotic acid solution cannot be obtained.

  When the high-concentration orotic acid solution of the present invention is used for beverage production, it is preferably sterilized in advance so that it can be added after diatomaceous earth filtration in the final step of beverage production, for example, beer. Examples thereof include heat treatment, aseptic filtration treatment and the like. However, since orotic acid and carnitine are stable to heat, heat treatment is preferable in terms of simplicity. Moreover, when using the high concentration orotic acid solution of this invention for manufacture of a drink, what does not contain the basic amino acid which causes bitterness is preferable.

  Especially as a manufacturing method of the orotic acid containing drink of this invention, if it is the method of adding the high concentration orotic acid solution of the said this invention to a drink in the ratio of 0.05-10 mass parts with respect to 100 mass parts of drinks. Although it does not restrict | limit, 0.1-5 mass parts is preferable with respect to 100 mass parts of drinks, and, as for the ratio of the high concentration orotic acid solution to add, 0.3-3 mass parts is more preferable, 0.5-2 mass parts Is particularly preferred.

  The orotic acid content in the orotic acid-containing beverage is 0.02 to 2% by mass, preferably 0.02 to 1% by mass, and more preferably 0.02 to 0.5% by mass. If the content of orotic acid is less than 0.02% by mass, it may be dissolved by orotic acid alone depending on the type of beverage. If it is more than 2% by mass, orotic acid may not be dissolved depending on the type of beverage. Or precipitation of orotic acid during storage. The content of carnitine in the orotic acid-containing beverage is 0.024 to 6% by mass, preferably 0.024 to 3% by mass, more preferably 0.024 to 2% by mass.

  The mass ratio of orotic acid and carnitine in the orotic acid-containing beverage is 1: 1.2 to 1:10, preferably 1: 1.5 to 1: 8, more preferably 1: 2 to 1: 5. Yes, particularly preferably 1: 3 to 1: 4. If the mass ratio is less than 1: 1.2, the effect of improving the solubility of orotic acid may not be obtained, and even if the mass ratio exceeds 1:10, the effect of further improving the solubility of orotic acid can be obtained. There is a risk that it will disappear, which is rather disadvantageous in terms of cost.

  The beverage in the present invention includes all of non-alcoholic beverages and alcoholic beverages. The beverage in the present invention includes health foods and functional foods in the form of beverages. Examples of the non-alcoholic beverage include mineral water, near water, sports drink, tea beverage, milk beverage, coffee beverage, fruit juice beverage, vegetable juice beverage, fruit juice and vegetable juice beverage, carbonated beverage, and the like. Can do. Furthermore, it can be used for beverages with a high solid content such as shakes and jelly, and beverages with high viscosity.

  The mineral water includes both foaming and non-foaming mineral water.

  The above tea drinks refer to drinks for decocting and drinking tea leaves (tea leaves), which are evergreen trees of the camellia family, or leaves or grains of plants other than tea trees. Fermented tea, semi-fermented tea, and non-fermented tea Any of these are included. Specific examples of such tea beverages include Japanese tea (for example, green tea, barley tea), black tea, herbal tea (for example, jasmine tea), Chinese tea (for example, Chinese green tea, oolong tea), houji tea, and the like.

  The above-mentioned milk beverages refer to beverages made mainly from raw milk, milk, etc. or foods made from these raw materials. In addition to those made from milk etc. itself, for example, nutrient-enriched milk, flavor-added milk, sugar decomposition Those made from processed milk such as milk are also included.

  Examples of the fruit used in the fruit juice-containing beverage or fruit juice and vegetable juice-containing beverage include apples, mandarin oranges, grapes, bananas, pears, peaches, mangoes, and the like. Moreover, as a vegetable used for a drink containing vegetable juice, fruit juice, and a drink containing vegetable juice, a tomato, a carrot, a celery, a pumpkin, a celery, a cucumber etc. can be mentioned, for example.

  Examples of the alcoholic beverage include whiskey, bourbon, spirits, liqueur, wine, fruit liquor, Japanese sake, Chinese liquor, shochu, beer, non-alcohol beer with an alcohol content of 1% or less, sparkling liquor, and high coffee.

  In the production of a beverage before the high-concentration orotic acid solution of the present invention is added, sugars, fragrances, fruit juices, food additives, and the like used in usual beverage formulation design can be added as appropriate. In the production of such beverages, production techniques known in the art can also be referred to, for example, “Revised New Version Soft Drinks” (Kokai Co., Ltd.) can be referred to.

  The container for filling the produced orotic acid-containing beverage may be any one that can fill the beverage, such as an aluminum can, a steel can, a PET bottle, and a paper container, and the capacity is not limited.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but these examples do not limit the present invention. In the following examples, orotic acid-free form was used as orotic acid, and L-carnitine-free form was used as carnitine. Moreover, all the concentrations (%) of orotic acid and carnitine mean mass%.

[Example 1]
The solubility (room temperature) of orotic acid by carnitine was investigated. The concentration of orotic acid is 0.5%, 1%, 2%, 5% and 10%, and the mass ratio of orotic acid and carnitine is 1: 1, 1: 1.1 for each concentration of orotic acid. , 1: 1.2, 1: 1.5 and 1: 2 were prepared, and the solubility of orotic acid by carnitine was examined. For example, 50 g of orotic acid was added to each beaker containing 500 mL of 10%, 11%, 12%, 15% and 20% solutions of carnitine, and the solubility at 10% orotic acid concentration was examined. The results are shown in Table 1. In Table 1, ◯ indicates dissolution, Δ indicates subtle precipitation, and X indicates precipitation.

  From Table 1, when the orotic acid concentration was 0.5%, there was no difference in solubility depending on the mixing ratio of orotic acid and carnitine, but when the orotic acid concentration was 1%, subtle precipitation occurred at a mixing ratio of 1: 1.1. It was found that the composition was dissolved at a mixing ratio of 1: 1.2. It was found that when the orotic acid concentration was 2%, fine precipitation occurred at a mixing ratio of 1: 1.2, and dissolution occurred at a mixing ratio of 1: 1.5. It was found that when the orotic acid concentrations were 5% and 10%, fine precipitation occurred at a blending ratio of 1: 1.5, and dissolution occurred at a blending ratio of 1: 2. As a result, in order to prepare a high concentration orotic acid solution of 2% to 10% at room temperature, carnitine is added to orotic acid so that the mass ratio of orotic acid and carnitine is at least 1: 1.2 or more. It turned out that it is necessary to mix | blend excessively.

  In addition, the solubility of orotic acid at high concentrations of 5%, 10% and 15% was examined again. A high-concentration carnitine solution (50%) is prepared in advance, and a predetermined amount of orotic acid and water are weighed so that the final concentrations of orotic acid are 5%, 10%, and 15%, respectively. Added and stirred to dissolve. Orotic acid 5% + carnitine 15% (1: 3), orotic acid 10% + carnitine 30% (1: 3), orotic acid 15% + carnitine 37.5% (1: 2.5), all dissolved. . Next, as a control, the solubility of orotic acid in which carnitine was replaced with lysine was examined. A high-concentration lysine solution (20%) is prepared in advance, and a predetermined amount of orotic acid and water are weighed so that the final concentrations of orotic acid are 3% and 5%, respectively, and added to the high-concentration lysine solution. And dissolved. Orotic acid 3% + lysine 9% (1: 3) dissolved, but orotic acid 5% + lysine 15% (1: 3) precipitated and did not dissolve at all.

  Next, the upper limit of dissolution of orotic acid was examined. The solubility of 20% orotic acid (orotic acid 20 g, carnitine 50 g, water 30 g) and 25% (orotic acid 25 g, carnitine 50 g, water 25 g) was examined. When stirring and dissolving while heating at 60 ° C., the orotic acid concentration of 20% was completely dissolved, but a little undissolved was observed at 25%. Thus, it was found that the upper limit of dissolution of orotic acid in the case of 50% carnitine was about 25%.

[Example 2]
The Maillard reaction is known as a reaction in which a carbonyl group of a reducing sugar and an amino group of a protein or amino acid react non-enzymatically to produce a brown pigment. It was examined whether beverages containing orotic acid and carnitine cause a Maillard reaction. As a control, a beverage containing orotic acid and lysine in which carnitine was replaced with lysine was used. A model beverage containing 0.5% orotic acid, 1% carnitine and 3% fructose glucose liquid sugar and a control beverage containing 0.5% orotic acid, 1% lysine and 3% fructose glucose liquid sugar were prepared. When each of these model beverages 100 g and control beverage 100 g was boiled for 20 minutes, as shown in FIG. 1, there was no change in the model beverage containing carnitine, but significant browning was observed in the control beverage containing lysine. . In this way, since Maillard reaction did not occur in carnitine, carnitine, which is not an amino acid, was used rather than basic amino acid in producing a high-concentration orotic acid solution that had been heat-treated by adding it to a beverage. It has been shown to be advantageous.

[Example 3]
A high-concentration orotic acid solution dissolved in carnitine or lysine is added to a commercially available sports drink. A drink having a final lysine concentration of 0.3% was prepared. The flavor was evaluated by three skilled panelists. The average value of 3 people was calculated based on 5 points of deliciousness, 3 points of deliciousness, 2 points to be able to drink somehow, and 1 point that was not delicious. On the other hand, in the case of orotic acid + lysine, only a low evaluation of 1.67 was obtained.

  The present invention is useful in the field of the beverage industry.

Claims (3)

High containing orotic acid in the range of 0.5 to 20% by mass and carnitine in the range of 0.6 to 50% by mass and containing orotic acid and carnitine in the range of 1: 1.2 to 1:10 by mass When the orotic acid concentration is 0.5 mass% or more and 1.0 mass% or less in the concentrated orotic acid solution , carnitine is 1.2 times or more of the orotic acid in a mass ratio, and the orotic acid concentration is 1.0 mass. at higher concentrations than%, if less than 2.0 wt%, carnitine mass ratio above 1.5 times the said orotic acid, at a higher concentration than orotic acid concentration of 2.0 wt%, 10.0 wt% for less, carnitine mass ratio of 2.0 times or more of the orotate, at higher concentrations than orotic acid concentration 10.0 wt%, in the case of less than 20.0 wt%, the carnitine in a weight ratio It is contained in the range of 2.5 times or more of orotic acid, A high concentration orotic acid solution with improved solubility and storage stability . The high-concentration orotic acid solution according to claim 1 , which has been sterilized by heat treatment. The high-concentration orotic acid solution according to claim 1 or 2 is added at a ratio of 0.05 to 10 parts by mass with respect to 100 parts by mass of the beverage during preparation of the beverage. Method.