JP6317770B2 - 高スループット精子選別用の装置及び方法 - Google Patents

高スループット精子選別用の装置及び方法 Download PDF

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JP6317770B2
JP6317770B2 JP2015561313A JP2015561313A JP6317770B2 JP 6317770 B2 JP6317770 B2 JP 6317770B2 JP 2015561313 A JP2015561313 A JP 2015561313A JP 2015561313 A JP2015561313 A JP 2015561313A JP 6317770 B2 JP6317770 B2 JP 6317770B2
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flow channel
sperm
channel
fluid
flow
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JP2016514955A (ja
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ジョナサン チャールズ シャープ，
ジョナサン チャールズ シャープ，
クリス ブキャナン，
クリス ブキャナン，
ネマニャ セドグラビッチ，
ネマニャ セドグラビッチ，
ブレア モラッド，
ブレア モラッド，
ドナルド フランシス ジュニア ペロー，
ドナルド フランシス ジュニア ペロー，
エリン コクサル，
エリン コクサル，
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イングラン， エルエルシー
イングラン， エルエルシー
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2015561313A 2013-03-14 2013-03-14 高スループット精子選別用の装置及び方法 Active JP6317770B2 (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2013/031706 WO2014142924A1 (fr) 2013-03-14 2013-03-14 Appareil et procédés pour tri de sperme à haut rendement

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JP2018067013A Division JP6621493B2 (ja) 2018-03-30 2018-03-30 高スループット精子選別用の装置及び方法

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JP2016514955A JP2016514955A (ja) 2016-05-26
JP6317770B2 true JP6317770B2 (ja) 2018-04-25

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EP (1) EP2969222A4 (fr)
JP (1) JP6317770B2 (fr)
KR (1) KR101920732B1 (fr)
CN (1) CN105073262B (fr)
BR (2) BR112015023155B1 (fr)
CA (1) CA2898740C (fr)
CL (1) CL2015002636A1 (fr)
IL (1) IL241159A0 (fr)
MX (1) MX2015012550A (fr)
NZ (1) NZ630559A (fr)
RU (1) RU2627379C2 (fr)
SG (1) SG11201505776YA (fr)
WO (1) WO2014142924A1 (fr)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012027366A2 (fr) 2010-08-23 2012-03-01 President And Fellows Of Harvard College Ondes acoustiques en microfluidique
CN105987870A (zh) * 2015-02-10 2016-10-05 博奥生物集团有限公司 一种流式细胞分选系统及其聚焦检测方法及其流体芯片
WO2016149639A1 (fr) * 2015-03-19 2016-09-22 The Board Of Trustees Of The Leland Stanford Junior University Dispositifs et procédés pour analyse et récupération à haut débit de biomolécules et de cellule unique dans une puce microfluidique
KR101954639B1 (ko) * 2015-06-25 2019-03-07 사이토놈/에스티, 엘엘씨 음향 조작을 이용하는 미세유체 장치 및 시스템
JP6657379B2 (ja) 2015-08-27 2020-03-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 弾性波による分離
JP6570765B2 (ja) * 2015-11-30 2019-09-04 ルクミクロ アーゲーＲｑｍｉｃｒｏ Ａｇ 試料から粒子を抽出するためのマイクロ流体デバイス、アセンブリおよび方法
CN108495922B (zh) * 2016-01-22 2022-10-28 小利兰·斯坦福大学托管委员会 一种用于从未处理的精液中选择性分选高活动和形态正常的精子的微流体装置
US10656070B2 (en) * 2016-03-30 2020-05-19 Siemens Healthcare Gmbh Aligning a non-spherical biological entity in a sample flow using ambient viscoelastic fluid flows
CN105831105B (zh) * 2016-04-12 2018-12-14 上海理工大学 微流体细胞处理芯片及其应用方法
CN109416314A (zh) * 2016-07-01 2019-03-01 生命技术公司 用于浓缩颗粒的方法、系统和装置
CN106807459B (zh) * 2016-12-13 2023-06-27 中国科学院苏州生物医学工程技术研究所 一种微流控芯片及其制备方法、应用
JP6919215B2 (ja) * 2017-02-17 2021-08-18 ソニーグループ株式会社 マイクロチップ及び微小粒子分取装置
CN107099444B (zh) * 2017-04-17 2024-04-09 隗慧林 精子分选芯片、精子检测设备及精子检测方法
GB2561587B (en) * 2017-04-19 2021-05-19 The Technology Partnership Plc Apparatus and method for sorting microfluidic particles
CN110945343B (zh) 2017-07-19 2023-01-13 英格朗公司 分析仪器和产生精子群体的方法
JP6871116B2 (ja) * 2017-09-15 2021-05-12 株式会社東芝 セルソータ
KR101966223B1 (ko) * 2017-09-29 2019-04-05 한국과학기술연구원 정자 관찰 구조체 및 이를 포함하는 정자 관찰 시스템
WO2019147289A1 (fr) 2018-01-29 2019-08-01 Hewlett-Packard Development Company, L.P. Catégorisation de particules
EP3772937A4 (fr) * 2018-04-09 2021-12-29 Inguran, LLC Noyaux de sperme améliorés et procédés d'obtention et d'utilisation de ces derniers
US10908065B2 (en) 2018-09-17 2021-02-02 Inguran, Llc Light collection from objects within a fluid column
JP7326787B2 (ja) * 2019-03-15 2023-08-16 株式会社リコー 選別装置
JP6661039B1 (ja) * 2019-03-28 2020-03-11 国立大学法人 東京大学 運動性細胞の選択装置
CN110302849B (zh) * 2019-05-31 2021-04-30 昆明理工大学 一种基于介电电泳原理分选液滴的微流控装置及方法
US11701658B2 (en) 2019-08-09 2023-07-18 President And Fellows Of Harvard College Systems and methods for microfluidic particle selection, encapsulation, and injection using surface acoustic waves
KR102378325B1 (ko) * 2019-11-22 2022-03-24 영남대학교 산학협력단 곡선 형태의 미세유체칩, 이의 제조방법 및 이를 이용한 정자를 선별하는 방법
WO2021145854A1 (fr) * 2020-01-13 2021-07-22 Abs Global, Inc. Puce microfluidique à gaine unique
CN111330657B (zh) * 2020-03-06 2021-12-31 上海材料研究所 一种基于相控阵超声波换能器的微流控装置
CN111323361B (zh) * 2020-03-17 2022-05-10 南通大学 一种快速分离精子头、精子尾和正常有活力精子的方法
CN111876327B (zh) * 2020-07-02 2022-12-02 清华大学 一种用于法医复杂样本精子快速分离的方法及其分离装置
KR102575032B1 (ko) * 2020-12-02 2023-09-07 한국전자통신연구원 가스 탐지 지능 학습 시스템 및 그의 동작 방법
IL305929A (en) * 2021-03-17 2023-11-01 Abs Global Inc Multidirectional light-based cell differentiation from a microfluidic chip
US11921026B2 (en) * 2021-04-16 2024-03-05 Cytonome/St, Llc Method and apparatus for an anti-sorting flow cytometer
CN114112826B (zh) * 2021-11-25 2024-04-19 天津大学 用于荧光微粒的声光互联微流控检测系统和检测方法
WO2024102007A1 (fr) * 2022-11-11 2024-05-16 Engender Technologies Limited Systèmes et procédés de tri et de classification de particules

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69028526T2 (de) * 1989-05-10 1997-02-06 The United States of America, represented by the Secretary, U.S. Department of Commerce, Washington, D.C. Verfahren zur vorwahl des geschlechts der nachkommenschaft
US6877528B2 (en) * 2002-04-17 2005-04-12 Cytonome, Inc. Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US6808075B2 (en) * 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
US20040043506A1 (en) 2002-08-30 2004-03-04 Horst Haussecker Cascaded hydrodynamic focusing in microfluidic channels
EP3511693B1 (fr) * 2003-03-28 2022-08-24 Inguran, LLC Dispositif pour détecter le point de détachement d'un système de production de gouttelettes
JP4533382B2 (ja) * 2003-08-28 2010-09-01 セルラ・インコーポレイテッド マイクロ流体分析およびソーティング用の一体化された構造物
WO2005042137A2 (fr) * 2003-10-30 2005-05-12 Cytonome, Inc. Structure de flux de gaine hydrodynamique multicouche
US20080272034A1 (en) * 2004-08-16 2008-11-06 Searete Llc, Separation of particles from a fluid by wave action
US7355696B2 (en) * 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
US7397232B2 (en) * 2005-10-21 2008-07-08 The University Of Akron Coulter counter having a plurality of channels
GB2464105A (en) * 2008-10-01 2010-04-07 Thorn Security A Particle Detector
JP5700189B2 (ja) * 2009-06-05 2015-04-15 公益財団法人かずさＤｎａ研究所 三次元シースフロー形成構造及び微粒子集束方法
CN103398936A (zh) * 2009-07-07 2013-11-20 索尼公司 微流体装置
WO2011097032A1 (fr) * 2010-02-05 2011-08-11 Cytonome/St, Llc Système d'analyse de particules à multiples canaux d'écoulement
US10481069B2 (en) * 2011-01-03 2019-11-19 Cytonome/St, Llc Method and apparatus for monitoring and optimizing microfluidic particle sorting
CA2826544C (fr) * 2011-02-04 2020-06-30 Cytonome/St, Llc Appareil et procede de triage de particules
CN108889345B (zh) * 2011-05-20 2020-10-09 布里格姆及妇女医院股份有限公司 游动细胞的分析和分选

Also Published As

Publication number Publication date
CA2898740C (fr) 2018-09-11
JP2016514955A (ja) 2016-05-26
CN105073262A (zh) 2015-11-18
WO2014142924A1 (fr) 2014-09-18
CN105073262B (zh) 2017-08-25
BR122016005348B1 (pt) 2023-01-24
SG11201505776YA (en) 2015-08-28
RU2627379C2 (ru) 2017-08-08
RU2015144002A (ru) 2017-04-20
CA2898740A1 (fr) 2014-09-18
BR112015023155A2 (pt) 2017-07-18
EP2969222A1 (fr) 2016-01-20
BR122016005348A2 (pt) 2018-06-19
IL241159A0 (en) 2015-11-30
KR20150130280A (ko) 2015-11-23
BR112015023155B1 (pt) 2022-09-20
MX2015012550A (es) 2016-02-10
EP2969222A4 (fr) 2016-11-16
NZ630559A (en) 2017-12-22
KR101920732B1 (ko) 2018-11-22
CL2015002636A1 (es) 2016-03-04

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